Pub Date : 2023-02-27DOI: 10.1080/10496475.2023.2184897
J. B. Adetunji, Abigail O. Agbolade, A. Iyoha, O. Adewale, Adeniyi S. Adefegha, Waliyat O. Salaudeen
ABSTRACT Background: This study investigated the Bryophyllum pinnatum live plant fractions’ effects on the activities of angiotensin-converting-enzyme (ACE), arginase, blood pressure, and biochemical parameters in sodium fluoride (NaF)-induced hypertensive rats. The results revealed that diastolic blood pressure (DBP), systolic blood pressure (SBP), mean arterial pressure (MAP), heart rate (HR), and arginase activity were reduced with increased nitric oxide level compared to hypertensive rats. The aqueous fraction (AQF), n-hexane fraction (NHF), and ethyl acetate fraction (EAF) boosted superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) levels with reduction in malondialdehyde (MDA) of hypertensive-treated rats. NHF portends the capability to inhibit ACE and arginase activities and enhanced the antioxidant biomarkers.
{"title":"Bryophyllum pinnatum Inhibits Angiotensin Converting Enzyme and Arginase Activities in Sodium Flouride Induced Hypertensive Rats","authors":"J. B. Adetunji, Abigail O. Agbolade, A. Iyoha, O. Adewale, Adeniyi S. Adefegha, Waliyat O. Salaudeen","doi":"10.1080/10496475.2023.2184897","DOIUrl":"https://doi.org/10.1080/10496475.2023.2184897","url":null,"abstract":"ABSTRACT Background: This study investigated the Bryophyllum pinnatum live plant fractions’ effects on the activities of angiotensin-converting-enzyme (ACE), arginase, blood pressure, and biochemical parameters in sodium fluoride (NaF)-induced hypertensive rats. The results revealed that diastolic blood pressure (DBP), systolic blood pressure (SBP), mean arterial pressure (MAP), heart rate (HR), and arginase activity were reduced with increased nitric oxide level compared to hypertensive rats. The aqueous fraction (AQF), n-hexane fraction (NHF), and ethyl acetate fraction (EAF) boosted superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) levels with reduction in malondialdehyde (MDA) of hypertensive-treated rats. NHF portends the capability to inhibit ACE and arginase activities and enhanced the antioxidant biomarkers.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"29 1","pages":"375 - 391"},"PeriodicalIF":0.0,"publicationDate":"2023-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44118527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-21DOI: 10.1080/10496475.2023.2182860
Ani Afriyanti, N. D. Yuliana, Yane Regiyana, H. Kusumaningrum
ABSTRACT This study determined the antibacterial activity of 12 minor Zingiberaceae spices and to identify the active compounds in the most active spices using chromatographic fingerprinting and multivariate data analysis. Curcuma purpurascens had the highest antibacterial activity against Staphylococcus aureus and Escherichia coli. HPLC chromatogram and antibacterial data of hexane, chloroform, ethyl acetate, methanol, and water extracts of C. purpurascens were linked using Orthogonal Projection to Latent Structure. Retention time of 16.5–18.0 min correlated with antibacterial activity and is abundantly found in methanol fraction. UHPLC-HRMS analysis of methanol fraction showed that the peak was attributed to demethoxycurcumin.
{"title":"Identification of Antibacterial Components in Indonesian Minor Spices Using Chromatographic Fingerprinting and Multivariate Data Analysis","authors":"Ani Afriyanti, N. D. Yuliana, Yane Regiyana, H. Kusumaningrum","doi":"10.1080/10496475.2023.2182860","DOIUrl":"https://doi.org/10.1080/10496475.2023.2182860","url":null,"abstract":"ABSTRACT This study determined the antibacterial activity of 12 minor Zingiberaceae spices and to identify the active compounds in the most active spices using chromatographic fingerprinting and multivariate data analysis. Curcuma purpurascens had the highest antibacterial activity against Staphylococcus aureus and Escherichia coli. HPLC chromatogram and antibacterial data of hexane, chloroform, ethyl acetate, methanol, and water extracts of C. purpurascens were linked using Orthogonal Projection to Latent Structure. Retention time of 16.5–18.0 min correlated with antibacterial activity and is abundantly found in methanol fraction. UHPLC-HRMS analysis of methanol fraction showed that the peak was attributed to demethoxycurcumin.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"29 1","pages":"356 - 374"},"PeriodicalIF":0.0,"publicationDate":"2023-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45836188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-20DOI: 10.1080/10496475.2023.2180698
O. N. Antosyuk, E. Bolotnik, D. V. Obvitsev
ABSTRACT The protective properties of the extract of Monarda fistulosa var. menthifolia were tested when used together with the antitumor drug etoposide on Drosophila melanogaster. 1%, 10% extracts of M. fistulosa var. menthifolia were tested, separately and together with etoposide at 800 µg kg−1 of nutrient medium. The genotoxic effect was determined using the SMART method. Cytotoxicity was determined by assessing regulated cell death (RCD). While the extract of M. fistulosa var. menthifolia at 1% (20 mg mL−1) did not show genotoxicity, and had antigenotoxicity, reducing the genetic activity of the anticancer drug etoposide, at 10% (200 mg mL−1) it showed geno- and cytotoxicity.
摘要:本文研究了薄荷蒙纳达提取物与抗肿瘤药物依托opo苷联合使用对黑腹果蝇的保护作用。在800µg kg - 1的营养培养基中，分别检测1%、10%薄荷提取物，并与依托泊苷一起检测。采用SMART法测定遗传毒性效应。通过评估调节细胞死亡(RCD)来确定细胞毒性。在1% (20 mg mL−1)浓度下，薄荷叶提取物无遗传毒性，具有抗遗传毒性，降低了抗癌药物etopo苷的遗传活性;在10% (200 mg mL−1)浓度下，薄荷叶提取物表现出基因毒性和细胞毒性。
{"title":"Testing the Protective Properties of the Extract of Monarda Fistulosa Var. Menthifolia L. Regarding the Effect of the Anticancer Drug Etoposide on the Example of Drosophila Melanogaster","authors":"O. N. Antosyuk, E. Bolotnik, D. V. Obvitsev","doi":"10.1080/10496475.2023.2180698","DOIUrl":"https://doi.org/10.1080/10496475.2023.2180698","url":null,"abstract":"ABSTRACT The protective properties of the extract of Monarda fistulosa var. menthifolia were tested when used together with the antitumor drug etoposide on Drosophila melanogaster. 1%, 10% extracts of M. fistulosa var. menthifolia were tested, separately and together with etoposide at 800 µg kg−1 of nutrient medium. The genotoxic effect was determined using the SMART method. Cytotoxicity was determined by assessing regulated cell death (RCD). While the extract of M. fistulosa var. menthifolia at 1% (20 mg mL−1) did not show genotoxicity, and had antigenotoxicity, reducing the genetic activity of the anticancer drug etoposide, at 10% (200 mg mL−1) it showed geno- and cytotoxicity. ","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"29 1","pages":"308 - 317"},"PeriodicalIF":0.0,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47554203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-05DOI: 10.1080/10496475.2022.2163734
Mary Zacharia Charwi, N. Mogha, J. K. Muluwa, K. Bostoen
ABSTRACT This study documented indigenous knowledge and use of medicinal plants among the Kuria communities in Mara Region, Tanzania. Ethnobotanical data were collected in collaboration with 20 traditional healers (THs), by jungle-walk-and-identify, field guides and observation, semi-structured interviews, focus group discussions and scientific identification of plants. Kuria medicinal plant healers reported 100 medicinal plants from 34 families. Asteraceae (15%) prevailed, followed by Fabaceae (13%) and Lamiaceae (12%). Herbs constituted the largest fraction (41%), followed by shrubs (27%), trees (24%), grasses (4%), climbers (4%), and ferns (1%). Leaves were the most used plant part. Healers listed about 53 diseases treated with plants.
{"title":"Indigenous Knowledge and Use of Medicinal Plants Among the Kuria Communities in the Tarime and Serengeti Districts of Mara Region, Tanzania","authors":"Mary Zacharia Charwi, N. Mogha, J. K. Muluwa, K. Bostoen","doi":"10.1080/10496475.2022.2163734","DOIUrl":"https://doi.org/10.1080/10496475.2022.2163734","url":null,"abstract":"ABSTRACT This study documented indigenous knowledge and use of medicinal plants among the Kuria communities in Mara Region, Tanzania. Ethnobotanical data were collected in collaboration with 20 traditional healers (THs), by jungle-walk-and-identify, field guides and observation, semi-structured interviews, focus group discussions and scientific identification of plants. Kuria medicinal plant healers reported 100 medicinal plants from 34 families. Asteraceae (15%) prevailed, followed by Fabaceae (13%) and Lamiaceae (12%). Herbs constituted the largest fraction (41%), followed by shrubs (27%), trees (24%), grasses (4%), climbers (4%), and ferns (1%). Leaves were the most used plant part. Healers listed about 53 diseases treated with plants.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"29 1","pages":"288 - 307"},"PeriodicalIF":0.0,"publicationDate":"2023-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47033899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-02DOI: 10.1080/10496475.2022.2087819
M. Salawu, Muhammed Iduze, A. Ayuba, H. Oloyede
ABSTRACT The protective effect of oral co-administration of Laurus nobilis on Escherichia coli endotoxin-exposed rabbits was studied. Twenty-five (25) rabbits were randomized into five groups containing five rabbits each – Group A, 500 Endotoxin Unit (EU) kg−1 bw, Group B, 500 EU kg−1 bw + 6.6 mg kg−1 bw extract, Group C, 500 EU kg−1 bw + 3.3 mg kg−1 bw extract, Group D, 500 EU kg−1 bw + Ascorbic acid (100 mg kg−1 bw) and Group E (0 EU kg−1 bw). The rabbits were pre-administered with the bay leaf extract in the first 7 days, then endotoxin oral administration was started and repeated daily for 21 d. At the end of days 7 and 21, assays for the serum superoxide dismutase, glutathione S-transferase, reduced glutathione, catalase, and malondialdehyde were done. The animals were sacrificed to collect kidneys and liver. Histopathological analysis of the liver and kidneys showed that exposure to 500 EU kg−1 bw endotoxin for 21 d + bay leaf extract at 6.6 and 3.3 mg kg−1 bw increased specific activities of superoxide dismutase, glutathione S-transferase, and reduced glutathione and catalase compared to the negative control. There was a decrease in the concentration of malondialdehyde in groups treated with the aqueous extract of bay leaf.
摘要研究了月桂口服联合给药对大肠杆菌内毒素暴露家兔的保护作用。二十五（25）只兔子被随机分为五组，每组五只兔子——A组，500内毒素单位（EU）kg−1 bw，B组，500 EU kg−1 bw+6.6 mg kg−1 bw提取物，C组，500 EUkg−1 BV+3.3 mg kg−一bw提取物，D组，500欧盟kg−1 GW+抗坏血酸（100 mg kg−1bw）和E组（0 EU kg−1BW）。在前7天给兔子预先给予月桂叶提取物，然后开始内毒素口服给药，每天重复给药21天。在第7天和第21天结束时，进行血清超氧化物歧化酶、谷胱甘肽S-转移酶、还原型谷胱甘肽、过氧化氢酶和丙二醛的测定。处死动物以收集肾脏和肝脏。肝脏和肾脏的组织病理学分析显示，与阴性对照相比，暴露于500 EU kg−1 bw内毒素的21 d+月桂叶提取物，剂量分别为6.6和3.3 mg kg−1 bw，可增加超氧化物歧化酶、谷胱甘肽S-转移酶、还原型谷胱甘肽和过氧化氢酶的比活性。在用月桂叶水提取物处理的组中丙二醛浓度降低。
{"title":"Protective Effects of Aqueous Extract of Bay Leaf (Laurus Nobilis) on Endotoxin-Induced Oxidative Stress in Rabbits","authors":"M. Salawu, Muhammed Iduze, A. Ayuba, H. Oloyede","doi":"10.1080/10496475.2022.2087819","DOIUrl":"https://doi.org/10.1080/10496475.2022.2087819","url":null,"abstract":"ABSTRACT The protective effect of oral co-administration of Laurus nobilis on Escherichia coli endotoxin-exposed rabbits was studied. Twenty-five (25) rabbits were randomized into five groups containing five rabbits each – Group A, 500 Endotoxin Unit (EU) kg−1 bw, Group B, 500 EU kg−1 bw + 6.6 mg kg−1 bw extract, Group C, 500 EU kg−1 bw + 3.3 mg kg−1 bw extract, Group D, 500 EU kg−1 bw + Ascorbic acid (100 mg kg−1 bw) and Group E (0 EU kg−1 bw). The rabbits were pre-administered with the bay leaf extract in the first 7 days, then endotoxin oral administration was started and repeated daily for 21 d. At the end of days 7 and 21, assays for the serum superoxide dismutase, glutathione S-transferase, reduced glutathione, catalase, and malondialdehyde were done. The animals were sacrificed to collect kidneys and liver. Histopathological analysis of the liver and kidneys showed that exposure to 500 EU kg−1 bw endotoxin for 21 d + bay leaf extract at 6.6 and 3.3 mg kg−1 bw increased specific activities of superoxide dismutase, glutathione S-transferase, and reduced glutathione and catalase compared to the negative control. There was a decrease in the concentration of malondialdehyde in groups treated with the aqueous extract of bay leaf.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"29 1","pages":"52 - 62"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45437650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-02DOI: 10.1080/10496475.2022.2086656
Amal Dhivahar S, Berlin Grace V M, David Wilson D, S. S.
ABSTRACT Flaxseed essential oil extracted by hydrodistillation was analyzed for its anticancer effects on A549 lung cancer cells. Phytochemicals were analyzed using Fourier-transform infrared spectroscopy (FTIR) and Gas Chromatography-Mass Spectrometry (GC-MS). The cytotoxicity effect was analyzed using Trypan blue, MTT, Clonogenic, DNA fragmentation, and wound scratch assays. Seventeen compounds with anticancer potency were identified. The cytotoxicity increased with increasing concentrations and inhibited migration of the cancer cells. Smeared DNA in gel and increased OD values compared to intact control DNA were also possible because of the phytochemicals in the essential oils.
{"title":"The Phytochemicals of Linum usitatissimum Seed Essential Oil Extracted by Hydrodistillation Induced Apoptosis and Suppressed Cell Migration in A549 Lung Cancer Cells","authors":"Amal Dhivahar S, Berlin Grace V M, David Wilson D, S. S.","doi":"10.1080/10496475.2022.2086656","DOIUrl":"https://doi.org/10.1080/10496475.2022.2086656","url":null,"abstract":"ABSTRACT Flaxseed essential oil extracted by hydrodistillation was analyzed for its anticancer effects on A549 lung cancer cells. Phytochemicals were analyzed using Fourier-transform infrared spectroscopy (FTIR) and Gas Chromatography-Mass Spectrometry (GC-MS). The cytotoxicity effect was analyzed using Trypan blue, MTT, Clonogenic, DNA fragmentation, and wound scratch assays. Seventeen compounds with anticancer potency were identified. The cytotoxicity increased with increasing concentrations and inhibited migration of the cancer cells. Smeared DNA in gel and increased OD values compared to intact control DNA were also possible because of the phytochemicals in the essential oils.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"29 1","pages":"39 - 51"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47223222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-11DOI: 10.1080/10496475.2022.2145592
Meranee Kidruangphokin, N. Suphrom, S. Boonphong
ABSTRACT The crude ethanolic extracts of different parts (root, stem, leaves and flower) of Lysiphyllum strychnifolium were tested for α-glucosidase inhibitory and antioxidant activities. Since all extracts showed good inhibitory activity, active constituents were isolated. Thirteen known compounds, including three dihydroflavanones, three dihydrochalcones, two stilbenes, flavan-3-ol, flavonol, cyclitol, phenolic ethyl ester and phenolic acid were isolated, and their α-glucosidase inhibitory effect and antioxidant activity were evaluated. Phloretin 4’-O-(6”-O-galloyl)-β-D-glucoside from the leaves extract showed potent α-glucosidase inhibitory activity (IC50 = 0.0162 ± 0.0014 mM) and potent antioxidant activity (IC50 = 19.26 ± 0.95 μM), each more potent than the positive controls. The compounds were investigated for their structure–activity relationship (SAR) in both activities. The number and position of hydroxy groups seemed to play a crucial role in increasing α-glucosidase inhibitory and antioxidant activities.
{"title":"α-Glucosidase Inhibitory and Antioxidant Activities of Ethanolic Extracts of Different Parts of Lysiphyllum strychnifolium and Their Constituents","authors":"Meranee Kidruangphokin, N. Suphrom, S. Boonphong","doi":"10.1080/10496475.2022.2145592","DOIUrl":"https://doi.org/10.1080/10496475.2022.2145592","url":null,"abstract":"ABSTRACT The crude ethanolic extracts of different parts (root, stem, leaves and flower) of Lysiphyllum strychnifolium were tested for α-glucosidase inhibitory and antioxidant activities. Since all extracts showed good inhibitory activity, active constituents were isolated. Thirteen known compounds, including three dihydroflavanones, three dihydrochalcones, two stilbenes, flavan-3-ol, flavonol, cyclitol, phenolic ethyl ester and phenolic acid were isolated, and their α-glucosidase inhibitory effect and antioxidant activity were evaluated. Phloretin 4’-O-(6”-O-galloyl)-β-D-glucoside from the leaves extract showed potent α-glucosidase inhibitory activity (IC50 = 0.0162 ± 0.0014 mM) and potent antioxidant activity (IC50 = 19.26 ± 0.95 μM), each more potent than the positive controls. The compounds were investigated for their structure–activity relationship (SAR) in both activities. The number and position of hydroxy groups seemed to play a crucial role in increasing α-glucosidase inhibitory and antioxidant activities.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"29 1","pages":"274 - 287"},"PeriodicalIF":0.0,"publicationDate":"2022-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49508994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-07DOI: 10.1080/10496475.2022.2143463
Shajaat Hussain, Nisha Kapoor, R. Mahajan
ABSTRACT A rapid and efficient micropropagation protocol was developed for Curcuma zedoaria, using shoot buds as explants. Explants established on basal MS medium were transferred to 12 different medium compositions with different combinations and concentrations of plant growth hormones 6-benzylaminopurine (BAP), kinetin (Kn), and thidiazuron (TDZ). The best shoot multiplication was recorded in MS medium supplemented with BAP (2.0 mg L−1) and TDZ (1.5 mg L−1), resulting in 5.3 ± 0.24 shoots of per explant and shoot length of 6.12 ± 0.19 cm. For the best in vitro root induction, the aseptic shoots excised from the culture flasks and inoculated individually on MS medium supplemented with 1.5 mg L−1 indole-3-butyric acid (IBA) resulted in 74.2% root growth and 8.82 ± 0.31 roots per explant. Although callus induction was observed in MS containing 2,4-dichlorophenoxyacetic acid (2, 4-D) and 1-naphthaleneacetic acid (NAA), the highest callus proliferation was observed with MS and NAA (2.0 mg L−1). Micropropagated plants established on soil showed 83% survival on acclimatization to the field conditions.
{"title":"High-frequency Shoot Multiplication and In Vitro Plantlet Regeneration from Shoot Bud Explants in Curcuma zedoaria Roscoe","authors":"Shajaat Hussain, Nisha Kapoor, R. Mahajan","doi":"10.1080/10496475.2022.2143463","DOIUrl":"https://doi.org/10.1080/10496475.2022.2143463","url":null,"abstract":"ABSTRACT A rapid and efficient micropropagation protocol was developed for Curcuma zedoaria, using shoot buds as explants. Explants established on basal MS medium were transferred to 12 different medium compositions with different combinations and concentrations of plant growth hormones 6-benzylaminopurine (BAP), kinetin (Kn), and thidiazuron (TDZ). The best shoot multiplication was recorded in MS medium supplemented with BAP (2.0 mg L−1) and TDZ (1.5 mg L−1), resulting in 5.3 ± 0.24 shoots of per explant and shoot length of 6.12 ± 0.19 cm. For the best in vitro root induction, the aseptic shoots excised from the culture flasks and inoculated individually on MS medium supplemented with 1.5 mg L−1 indole-3-butyric acid (IBA) resulted in 74.2% root growth and 8.82 ± 0.31 roots per explant. Although callus induction was observed in MS containing 2,4-dichlorophenoxyacetic acid (2, 4-D) and 1-naphthaleneacetic acid (NAA), the highest callus proliferation was observed with MS and NAA (2.0 mg L−1). Micropropagated plants established on soil showed 83% survival on acclimatization to the field conditions.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"29 1","pages":"262 - 273"},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47011161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-30DOI: 10.1080/10496475.2022.2140738
J. Mani, Joel B. Johnson, S. Bhattarai, Tieneke Trotter, M. Naiker
ABSTRACT The phytochemistry and anticancer properties of nine Nigella sativa genotypes grown in central Queensland, Australia, were determined and compared. The genotype AVTKS6 recorded the highest seed yield (2195 kg ha−1) and was associated with the highest plant density (112 plants m−2), whereas AVTKS8 had the highest harvest index (0.42). The seed yield was positively corrected with the plant density at harvest and capsules/plant at harvest, but not with the harvest index. All nine genotypes of N. sativa tested showed antioxidant potential (532–805 mg TXE 100 g−1) and total phenolic content (TPC) and ranged from 794 mg GAE 100 g−1 – 1126 mg GAE 100 g−1. The thymoquinone (TQ) content of nigella seeds varied between the genotypes, ranging from 0.896 (AVTKS4) – 1.728% w/w (AVTKS8). Seed yield was not correlated with TQ and TPC concentration, however, a negative correction between the seed yield and TAC was evident. Water extracts of the genotypes showed higher cytotoxic activity against the cell lines tested more prominently against HeLa cells (44–57% cell viability) compared to the MeOH extracts. Cytotoxic activity of MeOH extracts against HeLa cells were not different from the negative control and no activity was evident against the HT29 and PH5CH8 cell lines. However, PH5CH8 cells exposed to water extracts of AVTKS1, 3, 4, 5, 6, 7, and 8 showed only about 39–50% cell viability after 48 h of treatment, indicating that while the water extracts had beneficial cytotoxic activity against HeLa and HT29 cancer cell lines, it may also have toxic effects against healthy human cells. Principal component analysis of chemical and bioassays of the genotypes suggested that the AVTKS5 genotype was closely linked with desirable traits, highlighting it as an ideal cultivar.
{"title":"Phytochemistry and Therapeutical Potential of New Nigella Sativa Genotypes from Australia","authors":"J. Mani, Joel B. Johnson, S. Bhattarai, Tieneke Trotter, M. Naiker","doi":"10.1080/10496475.2022.2140738","DOIUrl":"https://doi.org/10.1080/10496475.2022.2140738","url":null,"abstract":"ABSTRACT The phytochemistry and anticancer properties of nine Nigella sativa genotypes grown in central Queensland, Australia, were determined and compared. The genotype AVTKS6 recorded the highest seed yield (2195 kg ha−1) and was associated with the highest plant density (112 plants m−2), whereas AVTKS8 had the highest harvest index (0.42). The seed yield was positively corrected with the plant density at harvest and capsules/plant at harvest, but not with the harvest index. All nine genotypes of N. sativa tested showed antioxidant potential (532–805 mg TXE 100 g−1) and total phenolic content (TPC) and ranged from 794 mg GAE 100 g−1 – 1126 mg GAE 100 g−1. The thymoquinone (TQ) content of nigella seeds varied between the genotypes, ranging from 0.896 (AVTKS4) – 1.728% w/w (AVTKS8). Seed yield was not correlated with TQ and TPC concentration, however, a negative correction between the seed yield and TAC was evident. Water extracts of the genotypes showed higher cytotoxic activity against the cell lines tested more prominently against HeLa cells (44–57% cell viability) compared to the MeOH extracts. Cytotoxic activity of MeOH extracts against HeLa cells were not different from the negative control and no activity was evident against the HT29 and PH5CH8 cell lines. However, PH5CH8 cells exposed to water extracts of AVTKS1, 3, 4, 5, 6, 7, and 8 showed only about 39–50% cell viability after 48 h of treatment, indicating that while the water extracts had beneficial cytotoxic activity against HeLa and HT29 cancer cell lines, it may also have toxic effects against healthy human cells. Principal component analysis of chemical and bioassays of the genotypes suggested that the AVTKS5 genotype was closely linked with desirable traits, highlighting it as an ideal cultivar.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"29 1","pages":"229 - 249"},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46840457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-20DOI: 10.1080/10496475.2022.2135661
M. P. Sobantu, B. Okeleye, V. Okudoh, M. Meyer, Yapo Guillaume Aboua
ABSTRACT The antioxidant mechanism of action in the process of cytotoxicity, and pro-apoptotic activity of Hibiscus sabdariffa water extract (HSWE) was assessed against non-cancerous (MCF12A) and breast cancer (MCF7) cells grown in DMEM-F12 and DMEM, respectively. The treated cells revealed a polyphenolic and anthocyanin content of 17.7 ± 0.4 mg g−1 and 24.8 ± 0.5 mg g,−1 respectively. The oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assay values were 1506 ± 30.1 µmoleTE g−1 and 428.9 ± 3.8 µmoleAAE g−1 at 1.0 mg mL−1, respectively, indicating a high antioxidant capacity. The HSWE induced greater apoptosis than doxorubicin (62.5 ± 2.6%), as more cytotoxic effect was noted against MCF7 with 70.4 ± 2.4% than MCF12A (15.3 ± 1.4%) cells at 0.4 mg mL−1. The HSWE depolarized mitochondrial MCF7 by 42.5 ± 0.5% compared to 4.6 ± 0.3% against MCF12A at 0.3 mg mL−1. It altered the intracellular redox status of MCF7 cells by depleting antioxidant enzymes with high levels of lipid peroxidation and reactive oxygen species [ROS] in MCF7 than in MCF12A. HSWE selectively induced apoptosis on MCF7 cells via ROS generation, lipid peroxidation, and mitochondrial dysfunction pathway.
摘要研究了芙蓉水提物(HSWE)对DMEM- f12和DMEM中生长的非癌细胞(MCF12A)和乳腺癌细胞(MCF7)的抗氧化作用机制和促凋亡活性。处理后的细胞多酚和花青素含量分别为17.7±0.4 mg g - 1和24.8±0.5 mg g - 1。在1.0 mg mL−1时，氧自由基吸收能力(ORAC)和铁还原抗氧化能力(FRAP)测定值分别为1506±30.1µmoleTE g−1和428.9±3.8µmoleAAE g−1，表明其具有较高的抗氧化能力。HSWE诱导的细胞凋亡高于阿霉素(62.5±2.6%)，对MCF7的细胞毒作用为70.4±2.4%，高于MCF12A(15.3±1.4%)。在0.3 mg mL−1时，HSWE对MCF12A去极化率为4.6±0.3%，而MCF7去极化率为42.5±0.5%。它通过消耗抗氧化酶来改变MCF7细胞的细胞内氧化还原状态，MCF7细胞的脂质过氧化和活性氧[ROS]水平高于MCF12A。HSWE通过ROS生成、脂质过氧化和线粒体功能障碍等途径选择性诱导MCF7细胞凋亡。
{"title":"In Vitro Antioxidant Mechanism of Action of Hibiscus Sabdariffa in the Induction of Apoptosis against Breast Cancer","authors":"M. P. Sobantu, B. Okeleye, V. Okudoh, M. Meyer, Yapo Guillaume Aboua","doi":"10.1080/10496475.2022.2135661","DOIUrl":"https://doi.org/10.1080/10496475.2022.2135661","url":null,"abstract":"ABSTRACT The antioxidant mechanism of action in the process of cytotoxicity, and pro-apoptotic activity of Hibiscus sabdariffa water extract (HSWE) was assessed against non-cancerous (MCF12A) and breast cancer (MCF7) cells grown in DMEM-F12 and DMEM, respectively. The treated cells revealed a polyphenolic and anthocyanin content of 17.7 ± 0.4 mg g−1 and 24.8 ± 0.5 mg g,−1 respectively. The oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assay values were 1506 ± 30.1 µmoleTE g−1 and 428.9 ± 3.8 µmoleAAE g−1 at 1.0 mg mL−1, respectively, indicating a high antioxidant capacity. The HSWE induced greater apoptosis than doxorubicin (62.5 ± 2.6%), as more cytotoxic effect was noted against MCF7 with 70.4 ± 2.4% than MCF12A (15.3 ± 1.4%) cells at 0.4 mg mL−1. The HSWE depolarized mitochondrial MCF7 by 42.5 ± 0.5% compared to 4.6 ± 0.3% against MCF12A at 0.3 mg mL−1. It altered the intracellular redox status of MCF7 cells by depleting antioxidant enzymes with high levels of lipid peroxidation and reactive oxygen species [ROS] in MCF7 than in MCF12A. HSWE selectively induced apoptosis on MCF7 cells via ROS generation, lipid peroxidation, and mitochondrial dysfunction pathway.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":"29 1","pages":"213 - 228"},"PeriodicalIF":0.0,"publicationDate":"2022-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45761131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：