Cells and Development最新文献

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Mucociliary cell type compositions - bridging the gap between genes and emergent tissue functions 粘液纤毛细胞类型组成-弥合基因和紧急组织功能之间的差距。

IF 2 4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Cells and Development

Pub Date : 2025-12-01 DOI: 10.1016/j.cdev.2025.204019

Peter Walentek

When multiple cell types are brought together to form a tissue-specific collective, the combination of cell functions and cell-cell interactions leads to novel behaviors and properties beyond the simple addition of individual features, often referred to as emergent tissue functions. During evolution, functional adaptations in organs are significantly influenced by changes in cell type compositions, and in diseases, aberrations in cell type compositions result in impaired organ functions. Investigating the mechanisms that regulate cell type compositions could elucidate an important organizational meta-level that bridges gene functions and cellular features de facto facilitating the emergence of collective cell behaviors and novel tissue functions. Due to their unique evolutionary positioning and diverse functions, mucociliary epithelia could provide an optimal system to unravel principle mechanisms of adaptations in cell type compositions that facilitate the evolution of new or optimization of existing tissue functions, and could reveal novel entry points to counteract human diseases. An integrative investigation of signaling, transcriptional, epigenetic and morphogenetic mechanisms across a broad range of mucociliary tissues with different specialized cells and cell type compositions can help us to connect gene functions and contributions to self-organized behaviors in cell collectives determining emergent tissue functions. Taking such route moving forward has the potential to unravel novel principles in mucociliary self-organization and to reveal broadly applicable principles underlying the generation and modification of emergent tissue functions across species and organ systems.

当多种细胞类型聚集在一起形成一个组织特异性集体时，细胞功能和细胞间相互作用的结合导致了新的行为和特性，而不仅仅是单个特征的简单添加，通常被称为突发性组织功能。在进化过程中，器官的功能适应受到细胞类型组成变化的显著影响，在疾病中，细胞类型组成的畸变导致器官功能受损。研究调节细胞类型组成的机制可以阐明一个重要的组织元水平，它连接基因功能和细胞特征，促进集体细胞行为和新组织功能的出现。由于其独特的进化定位和多样的功能，粘液纤毛上皮可以提供一个最佳系统来揭示细胞类型组成的适应原理机制，促进新的或优化现有组织功能的进化，并可能揭示对抗人类疾病的新切入点。通过对具有不同特化细胞和细胞类型组成的粘膜纤毛组织的信号传导、转录、表观遗传和形态发生机制的综合研究，可以帮助我们将基因功能与细胞集体中决定紧急组织功能的自组织行为联系起来。沿着这条路线向前发展，有可能揭示粘膜纤毛自组织的新原理，并揭示跨物种和器官系统产生和修改紧急组织功能的广泛适用原理。

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引用次数: 0

Self-organization underlies developmental robustness in plants 自组织是植物发育稳健性的基础。

IF 2 4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Cells and Development

Pub Date : 2025-12-01 DOI: 10.1016/j.cdev.2024.203936

Shuyao Kong , Mingyuan Zhu , Adrienne H.K. Roeder

Development is a self-organized process that builds on cells and their interactions. Cells are heterogeneous in gene expression, growth, and division; yet how development is robust despite such heterogeneity is a fascinating question. Here, we review recent progress on this topic, highlighting how developmental robustness is achieved through self-organization. We will first discuss sources of heterogeneity, including stochastic gene expression, heterogeneity in growth rate and direction, and heterogeneity in division rate and precision. We then discuss cellular mechanisms that buffer against such noise, including Paf1C- and miRNA-mediated denoising, spatiotemporal growth averaging and compensation, mechanisms to improve cell division precision, and coordination of growth rate and developmental timing between different parts of an organ. We also discuss cases where such heterogeneity is not buffered but utilized for development. Finally, we highlight potential directions for future studies of noise and developmental robustness.

发育是一个建立在细胞及其相互作用基础上的自组织过程。细胞在基因表达、生长和分裂方面存在异质性；然而，尽管存在这种异质性，发育如何保持稳健是一个引人入胜的问题。在此，我们将回顾这一课题的最新进展，重点介绍如何通过自组织实现发育的稳健性。我们将首先讨论异质性的来源，包括随机基因表达、生长速度和方向的异质性以及分裂速度和精确度的异质性。然后，我们将讨论缓冲这些噪音的细胞机制，包括由 Paf1C 和 miRNA 介导的去噪、时空生长平均化和补偿、提高细胞分裂精确度的机制，以及协调器官不同部位之间的生长速度和发育时间。我们还讨论了这种异质性不是被缓冲而是被用于发育的情况。最后，我们强调了未来研究噪声和发育稳健性的潜在方向。

引用次数: 0

Dorsoventral patterning beyond the gastrulation stage: Interpretation of early dorsoventral cues and modular development mediated by zic1/zic4 原肠胚期后的背腹侧模式：zic1/zic4介导的早期背腹侧线索和模块化发育的解释。

IF 2 4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Cells and Development

Pub Date : 2025-12-01 DOI: 10.1016/j.cdev.2025.204012

Toru Kawanishi , Hiroyuki Takeda

Dorsoventral (DV) patterning is fundamental to vertebrate development, organizing the entire body across different germ layers. Although early DV axis formation, centered on the Spemann-Mangold organizer through the BMP activity gradient, has been extensively studied, the mechanisms shaping DV traits during later development remain largely unexplored. In this review, we highlight recent findings, especially from studies involving the Double anal fin (Da) spontaneous mutant of the small teleost medaka (Oryzias latipes), focusing on the roles of zic1 and zic4 (zic1/zic4) in regulating late DV patterning. These genes establish the dorsal domain of the trunk by converting the initial BMP gradient into distinct on/off spatial compartments within somites and their derivatives, acting as selector genes that define dorsal-specific traits, including myotome structure, body shape, and dorsal fin development. We also discuss how the zic-mediated dorsal domain is established and maintained from embryogenesis through adulthood. Furthermore, we provide evidence that zic-dependent action on the dorsal characteristics is dosage-dependent. We propose that the zic1/zic4-mediated DV patterning mechanism may represent a conserved regulatory framework that has been adapted to support the diverse body plans observed across vertebrate species.

背腹（DV）模式是脊椎动物发育的基础，横跨不同的胚层组织整个身体。尽管以Spemann-Mangold组织者为中心，通过BMP活性梯度形成的早期DV轴形成已被广泛研究，但在后期发育过程中形成DV特征的机制仍未被探索。在这篇综述中，我们重点介绍了最近的研究结果，特别是涉及小硬骨鱼（Oryzias latipes）双肛鳍（Da）自发突变体的研究，重点介绍了zic1和zic4 （zic1/zic4）在调节晚期DV模式中的作用。这些基因通过将初始BMP梯度转化为体及其衍生物中不同的开/关空间区来建立躯干的背域，并作为选择基因定义背特异性特征，包括肌组结构、体型和背鳍发育。我们还讨论了锌介导的背结构域是如何从胚胎发育到成年建立和维持的。此外，我们提供的证据表明，锌对背侧特征的依赖作用是剂量依赖的。我们提出，zic1/zic4介导的DV模式机制可能代表了一个保守的调节框架，该框架已被适应以支持在脊椎动物物种中观察到的多种身体计划。

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引用次数: 0

Coordinating tissue development to build body and brain: Lessons from the gastrula organiser and its derivatives 协调组织发育以构建身体和大脑：来自原肠腺组织者及其衍生物的教训。

IF 2 4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Cells and Development

Pub Date : 2025-12-01 DOI: 10.1016/j.cdev.2025.204042

Alexandra Neaverson , Benjamin Steventon

The Spemann-Mangold organiser has been a central paradigm in developmental biology for over a century, primarily studied through its ability to induce secondary axes in transplantation experiments. However, how the organiser and its derivatives act together to generate a well-proportioned body axis is still unknown. Here, we review evidence across four major vertebrate models (Xenopus, zebrafish, chick, and mouse) to evaluate the organiser's stage-specific requirements in neural development and body axis formation. We present a consensus view that initial neural specification and anterior-posterior patterning occur before morphological organiser formation, driven by planar signals from pre-organiser cells and species-specific contributions from extraembryonic tissues. We also discuss how the organiser-derived anterior axial mesendoderm (prechordal plate and head process) primarily acts to maintain anterior identity and ensure proper tissue proportions through continued signalling. The posterior organiser derivative, the notochord, remains essential for dorsal-ventral neural patterning, but also coordinates posterior body elongation through mechanical coupling with adjacent tissues, and the pace of axial mesoderm extension serves as a key coordinator of proportional body axis development. Future studies will improve our understanding of how organiser derivatives orchestrate the mechanical and molecular coordination of multiple tissues during axis formation.

一个多世纪以来，Spemann-Mangold组织者一直是发育生物学的中心范式，主要通过其在移植实验中诱导次级轴的能力进行研究。然而，组织者及其衍生物如何共同作用以产生匀称的身体轴仍是未知的。在这里，我们回顾了四种主要脊椎动物模型（爪蟾、斑马鱼、小鸡和老鼠）的证据，以评估组织者在神经发育和身体轴形成方面的阶段特异性要求。我们提出了一个共识的观点，即最初的神经规范和前后模式发生在形态组织者形成之前，由来自前组织者细胞的平面信号和来自胚胎外组织的物种特异性贡献驱动。我们还讨论了组织者衍生的前轴中胚层（脊索前板和头突）如何主要通过持续的信号传导维持前轴身份并确保适当的组织比例。后组织者衍生物脊索对背-腹侧神经模式仍然至关重要，但也通过与邻近组织的机械耦合协调后体延伸，轴向中胚层延伸的速度是比例体轴发育的关键协调者。未来的研究将提高我们对组织者衍生物在轴形成过程中如何协调多个组织的机械和分子协调的理解。

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引用次数: 0

Homeotic transformation of tail to limbs: A novel morphogenesis in the framework of self-organization and reprogramming of cell fate during appendage regeneration 尾巴到四肢的同源转化：在附属物再生过程中自组织和细胞命运重编程框架下的一种新的形态发生。

IF 2 4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Cells and Development

Pub Date : 2025-12-01 DOI: 10.1016/j.cdev.2024.203987

Jutshina Hota , Swetamudra Pattnaik , Gunanidhi Sahoo , Priyambada Mohanty-Hejmadi , Pravati Kumari Mahapatra

Homeotic transformation of tail to hindlimbs in anuran tadpoles is a manifestation of the reprogramming of positional information in the event of tail regeneration. Such discovery of homeosis is of particular interest considering its occurrence in a vertebrate under the influence of a morphogen which represents a self-organizing system in the context of developmental and regenerative studies. This article reviews homeotic transformation of tail to hindlimbs including pelvic girdles induced by retinoic acid (RA) /vitamin A palmitate during tail regeneration under the scope of self-organization and the role of blastema as an organizer. Next, we present a timeline of various findings in this context.

无尾蝌蚪尾巴向后肢的同位化是尾巴再生过程中位置信息重编程的一种表现。在发育和再生研究的背景下，这种同型异构体的发现是特别有趣的，因为它发生在一种受形态原影响的脊椎动物中，形态原代表着一种自组织系统。本文综述了维甲酸(RA) /棕榈酸维生素A在自组织范围内诱导尾巴向包括骨盆带在内的后肢的同源转化以及胚基作为组织者的作用。接下来，我们将介绍在此背景下的各种发现的时间表。

引用次数: 0

Organizer activity in the mouse embryo 小鼠胚胎中的组织者活动。

IF 2 4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Cells and Development

Pub Date : 2025-12-01 DOI: 10.1016/j.cdev.2025.204001

Jenny Kretzschmar , Katharine Goodwin , Katie McDole

The discovery of the embryonic organizer by Hilde Mangold and Hans Spemann in 1924 was one of the most ground-breaking achievements in the 1900 century for developmental biologists and beyond. Ever since the organizer was first described in newts, developmental biologists have been trying to uncover similar structures in other organisms. While the Spemann-Mangold organizer as an axis-inducing centre is evolutionary conserved in vertebrates, similar organizing centres have yet to be observed in mammals. In this review, we will provide a brief historical overview of the discovery of the mouse gastrula organizer and discuss its potential as an organizer throughout early post-implantation mouse development. We discuss cell migrations through the mouse organizer region and morphogenesis of organizer cells and tissues. Finally, we examine the evidence arguing for and against the existence of a head organizer in mice, and the role of the anterior visceral endoderm and the prechordal plate in organizing head structures.

希尔德·曼戈尔德和汉斯·斯佩曼在1924年发现了胚胎组织者，这是20世纪对发育生物学家及以后的人来说最具开创性的成就之一。自从在蝾螈中首次描述了组织者以来，发育生物学家一直试图在其他生物体中发现类似的结构。虽然Spemann-Mangold组织者作为轴诱导中心在脊椎动物中是进化保守的，但在哺乳动物中尚未观察到类似的组织中心。在这篇综述中，我们将简要介绍小鼠原肠组织体的发现历史，并讨论其在早期植入后小鼠发育过程中作为组织体的潜力。我们讨论了细胞迁移通过小鼠组织者区域和组织者细胞和组织的形态发生。最后，我们研究了支持和反对小鼠头部组织存在的证据，以及前内脏内胚层和脊索前板在组织头部结构中的作用。

引用次数: 0

Butterfly pupal wing tissue with an eyespot organizer 带眼斑组织的蝴蝶蛹翅膀组织。

IF 2 4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Cells and Development

Pub Date : 2025-12-01 DOI: 10.1016/j.cdev.2024.203992

Joji M. Otaki , Atsuko Tanaka , Euichi Hirose

Butterfly wing eyespots are developmentally determined at the early pupal stage, when prospective eyespot focal cells underneath the pupal cuticle focal spot function as eyespot organizers in the pupal wing tissue. Here, we performed light microscopy and transmission electron microscopy (TEM) to describe cellular structures of pupal wing tissue with an eyespot organizer immediately after pupation using the Blue Pansy butterfly Junonia orithya. The pupal forewing dorsal epidermis was a pseudostratified monolayer of vertically elongated epidermal cells. The apical portion of the cells adhered laterally to one another, but their medial and basal portions were thinner than the apical portion and were tilted to enclose cells at the center, forming a cellular cluster. The cellular cluster at the organizer was relatively large laterally and vertically. The apical portion of the cells and its corresponding cuticle at the organizer were thicker than those in the surroundings. The innermost cuticle layer was being synthesized, indicating high cuticle synthesis and secretion activity of the cells. At the medial and basal portions of the dorsal epidermis, there were many intracellular and extracellular vacuole-like globules, most likely containing extracellular matrix molecules. Some of the basal processes from epidermal cells extended to form protrusions of the basement membrane, which was often attended by hemocytes. These results suggest that the butterfly eyespot organizer is composed of a single or a few cellular clusters that secrete more cuticle than surrounding clusters, supporting the pupal cuticle hypothesis that cuticle formation is critical for eyespot color pattern determination in butterflies.

蝴蝶翅膀上的眼斑是在蛹早期发育决定的，蛹表皮下的眼斑细胞在蛹翅组织中起着眼斑组织者的作用。在这里，我们使用光学显微镜和透射电子显微镜（TEM）来描述蛹化蛹后立即用眼斑组织者的翅膀组织的细胞结构，使用蓝三色蝶Junonia算法。蛹前翅背表皮是由垂直伸长的表皮细胞组成的假层状单层。细胞的根尖部相互横向粘附，但其内侧和基部比根尖部薄，并在中心倾斜包围细胞，形成细胞簇。在组织者处的细胞簇在横向和纵向上相对较大。细胞器顶端的细胞及其角质层比周围的细胞更厚。最内层角质层正在合成，表明细胞的角质层合成和分泌活性较高。在背表皮的内侧和基部，有许多细胞内和细胞外的液泡状小球，很可能含有细胞外基质分子。一些来自表皮细胞的基突延伸形成基底膜的突起，通常有血细胞参与。这些结果表明，蝴蝶的眼斑组织者是由单个或几个细胞簇组成的，这些细胞簇比周围的细胞簇分泌更多的角质层，这支持了蛹角质层的假设，即角质层的形成对蝴蝶的眼斑颜色图案的决定至关重要。

{"title":"Butterfly pupal wing tissue with an eyespot organizer","authors":"Joji M. Otaki , Atsuko Tanaka , Euichi Hirose","doi":"10.1016/j.cdev.2024.203992","DOIUrl":"10.1016/j.cdev.2024.203992","url":null,"abstract":"<div><div>Butterfly wing eyespots are developmentally determined at the early pupal stage, when prospective eyespot focal cells underneath the pupal cuticle focal spot function as eyespot organizers in the pupal wing tissue. Here, we performed light microscopy and transmission electron microscopy (TEM) to describe cellular structures of pupal wing tissue with an eyespot organizer immediately after pupation using the Blue Pansy butterfly <em>Junonia orithya</em>. The pupal forewing dorsal epidermis was a pseudostratified monolayer of vertically elongated epidermal cells. The apical portion of the cells adhered laterally to one another, but their medial and basal portions were thinner than the apical portion and were tilted to enclose cells at the center, forming a cellular cluster. The cellular cluster at the organizer was relatively large laterally and vertically. The apical portion of the cells and its corresponding cuticle at the organizer were thicker than those in the surroundings. The innermost cuticle layer was being synthesized, indicating high cuticle synthesis and secretion activity of the cells. At the medial and basal portions of the dorsal epidermis, there were many intracellular and extracellular vacuole-like globules, most likely containing extracellular matrix molecules. Some of the basal processes from epidermal cells extended to form protrusions of the basement membrane, which was often attended by hemocytes. These results suggest that the butterfly eyespot organizer is composed of a single or a few cellular clusters that secrete more cuticle than surrounding clusters, supporting the pupal cuticle hypothesis that cuticle formation is critical for eyespot color pattern determination in butterflies.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"184 ","pages":"Article 203992"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-organization of an organizer: Whole-body regeneration from reaggregated cells in cnidarians 组织者的自组织：刺胞动物中重新聚集的细胞的全身再生。

IF 2 4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Cells and Development

Pub Date : 2025-12-01 DOI: 10.1016/j.cdev.2025.204024

Sanjay Narayanaswamy, Ulrich Technau

Cnidarians like the freshwater polyp Hydra and the sea anemone Nematostella, are famous for their enormous capacity to regenerate missing head or feet upon bisection. Classical transplantation experiments have demonstrated that the hypostome, the oral tip of the freshwater polyp Hydra, acts as an axial organizer. Likewise, transplantation of the blastopore lip of an early Nematostella gastrula stage embryo to an aboral position leads to ectopic head formation. Following molecular analyses have shown that Wnt signaling is the key component of this organizer activity. Moreover, when dissociated and reaggregated head (and foot) organizer centres are re-established by self-organization. Similarly, “gastruloids”, i.e. aggregates of dissociated early gastrula stage embryos, are able to self-organize. Here, we review the past and recent molecular and theoretical work in the field to explain this phenomenon. While Turing-type reaction-diffusion models involving morphogens like Wnt dominated the field for many years, recent work emphasized the importance of biophysical cues in symmetry breaking and establishment of the organizers in aggregates. The comparison with Nematostella aggregates suggests that the principles of self-organization in cnidarians is not universal.

淡水多足类水螅和海葵等无脊椎动物因其巨大的再生能力而闻名，缺失的头或足在断裂后可以再生。经典的移植实验证明，淡水多足类水螅的口部顶端（hypostome）具有轴向组织器的作用。同样，将早期 Nematostella gastrula 阶段胚胎的胚泡唇移植到口部位置可导致异位头部形成。随后的分子分析表明，Wnt 信号是这种组织器活动的关键组成部分。此外，当头（和足）组织者中心解离并重新聚集时，它们会通过自组织重新建立。同样，"胃小体"，即解离的早期胃小体阶段胚胎的聚集体，也能够自组织。在此，我们回顾了该领域过去和近期的分子和理论工作，以解释这一现象。多年来，涉及 Wnt 等形态诱导因子的图灵型反应-扩散模型一直主导着这一领域，而最近的工作则强调了生物物理线索在打破对称性和建立聚集体组织者中的重要性。与线虫聚集体的比较表明，刺胞动物的自组织原理并不具有普遍性。

{"title":"Self-organization of an organizer: Whole-body regeneration from reaggregated cells in cnidarians","authors":"Sanjay Narayanaswamy, Ulrich Technau","doi":"10.1016/j.cdev.2025.204024","DOIUrl":"10.1016/j.cdev.2025.204024","url":null,"abstract":"<div><div>Cnidarians like the freshwater polyp <em>Hydra</em> and the sea anemone <em>Nematostella</em>, are famous for their enormous capacity to regenerate missing head or feet upon bisection. Classical transplantation experiments have demonstrated that the hypostome, the oral tip of the freshwater polyp <em>Hydra</em>, acts as an axial organizer. Likewise, transplantation of the blastopore lip of an early <em>Nematostella</em> gastrula stage embryo to an aboral position leads to ectopic head formation. Following molecular analyses have shown that Wnt signaling is the key component of this organizer activity. Moreover, when dissociated and reaggregated head (and foot) organizer centres are re-established by self-organization. Similarly, “gastruloids”, <em>i.e.</em> aggregates of dissociated early gastrula stage embryos, are able to self-organize. Here, we review the past and recent molecular and theoretical work in the field to explain this phenomenon. While Turing-type reaction-diffusion models involving morphogens like Wnt dominated the field for many years, recent work emphasized the importance of biophysical cues in symmetry breaking and establishment of the organizers in aggregates. The comparison with <em>Nematostella</em> aggregates suggests that the principles of self-organization in cnidarians is not universal.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"184 ","pages":"Article 204024"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional rosettes in epithelial formation 上皮形成中的三维莲座。

IF 2 4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Cells and Development

Pub Date : 2025-12-01 DOI: 10.1016/j.cdev.2025.204022

Lila Neahring, Jennifer A. Zallen

Epithelia are ubiquitous tissues with essential structural, signaling, and barrier functions. How cells transition from individual to collective behaviors as they build and remodel epithelia throughout development is a fundamental question in developmental biology. Recent studies show that three-dimensional multicellular rosettes are key intermediates that provide a solution to the challenge of building tissue-scale epithelia by coordinating local interactions in small groups of cells. These radially polarized rosette structures facilitate epithelial formation by providing a protected environment for cells to acquire apical-basal polarity, establish cell adhesion, and coordinate intercellular signaling. Once formed, rosettes can dynamically expand, move, coalesce, and interact with surrounding tissues to generate a wide range of structures with specialized functions, including epithelial sheets, tubes, cavities, and branched networks. In this review, we describe the mechanisms that regulate rosette assembly and dynamics, and discuss how rosettes serve as versatile intermediates in epithelial morphogenesis. In addition, we present open questions about the molecular, cellular, and biophysical mechanisms that drive rosette behaviors, and discuss the implications of this widely used mode of epithelial formation for understanding embryonic development and human disease.

上皮是普遍存在的组织，具有重要的结构、信号和屏障功能。在整个发育过程中，单个细胞如何共同作用来组装和重塑上皮是发育生物学的一个基本问题。最近的研究表明，三维多细胞玫瑰花是关键的中间体，通过协调细胞群内的局部相互作用，促进上皮性质的合作获取，为构建新上皮提供了解决方案。在一些物种的新生上皮中观察到莲座，由细胞群组成，细胞连接和顶基极性围绕中心管腔呈放射状组织。一旦形成，莲座可以动态扩展、移动、合并，并与周围组织相互作用，产生广泛的上皮结构，包括片状、管状、空腔和分支网络。在这篇综述中，我们描述了调节玫瑰结组装和动力学的细胞和分子机制，并讨论了玫瑰结如何作为多功能中间体产生各种上皮形态。此外，我们提出了关于启动和组织玫瑰结行为的机制的开放性问题，并讨论了这种上皮形成的集体模式对理解胚胎发育和人类疾病的意义。

{"title":"Three-dimensional rosettes in epithelial formation","authors":"Lila Neahring, Jennifer A. Zallen","doi":"10.1016/j.cdev.2025.204022","DOIUrl":"10.1016/j.cdev.2025.204022","url":null,"abstract":"<div><div>Epithelia are ubiquitous tissues with essential structural, signaling, and barrier functions. How cells transition from individual to collective behaviors as they build and remodel epithelia throughout development is a fundamental question in developmental biology. Recent studies show that three-dimensional multicellular rosettes are key intermediates that provide a solution to the challenge of building tissue-scale epithelia by coordinating local interactions in small groups of cells. These radially polarized rosette structures facilitate epithelial formation by providing a protected environment for cells to acquire apical-basal polarity, establish cell adhesion, and coordinate intercellular signaling. Once formed, rosettes can dynamically expand, move, coalesce, and interact with surrounding tissues to generate a wide range of structures with specialized functions, including epithelial sheets, tubes, cavities, and branched networks. In this review, we describe the mechanisms that regulate rosette assembly and dynamics, and discuss how rosettes serve as versatile intermediates in epithelial morphogenesis. In addition, we present open questions about the molecular, cellular, and biophysical mechanisms that drive rosette behaviors, and discuss the implications of this widely used mode of epithelial formation for understanding embryonic development and human disease.</div></div>","PeriodicalId":36123,"journal":{"name":"Cells and Development","volume":"184 ","pages":"Article 204022"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Celebrating a century of the Spemann-mangold experiment: Self-organisation in biology 庆祝Spemann-mangold实验一百周年：生物学中的自组织。

IF 2 4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Cells and Development

Pub Date : 2025-12-01 DOI: 10.1016/j.cdev.2025.204061

Roberto Mayor

引用次数: 0

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