Pub Date : 2023-12-31DOI: 10.5423/rpd.2023.29.4.405
Hyun Su Kang, Mi-Jin Kim, Y. Shin, Y. Jeun
Bacterial blight of carrot caused by Xanthomonas hortorum pv. carotae (Xhc) is one of the serious diseases of carrot, of which control measures has not been still established in the domestic farm. In this study, in order to select effective sterilizer for bacterial blight of carrots, three antibiotics such as streptomycin, oxolinic acid, kasugamycin, two copper compounds like copper hydroxide and copper sulfate basic and three rhizobacteria Burkholderia gladioli MRL408-3, Pseudomonas fluorescens TRH415-2 and Bacillus cereus KRY505-3 were selected to investigate their direct antibacterial effects using artificial media, aiming to identify effective pesticides against Xhc. Among them, treated medium with antibiotics such as streptomycin, oxolinic acid, and the antagonistic rhizobacteria MRL408-3 were formed inhibition zone. The agrochemicals and the rhizobacteria MRL408-3, which showed antibacterial effects on carrot leaves, pre-treated on the carrot leaves and then inoculated with Xhc. High control effects were shown on the carrot leaves pre-treated with both streptomycin and oxolinic acid. Scanning electron microscopy images of the carrot leaf surfaces showed that the population of bacteria decreased significantly on leaves pre-treated with streptomycin and oxolinic acid. From these results, it can be inferred that antibiotics like streptomycin and oxolinic acid exhibit superior control effects compared to other agents. This study provides valuable insights towards establishing an effective control system for bacterial blight of carrot.
{"title":"Effective Control Strategy against Bacterial Blight on Carrot","authors":"Hyun Su Kang, Mi-Jin Kim, Y. Shin, Y. Jeun","doi":"10.5423/rpd.2023.29.4.405","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.4.405","url":null,"abstract":"Bacterial blight of carrot caused by Xanthomonas hortorum pv. carotae (Xhc) is one of the serious diseases of carrot, of which control measures has not been still established in the domestic farm. In this study, in order to select effective sterilizer for bacterial blight of carrots, three antibiotics such as streptomycin, oxolinic acid, kasugamycin, two copper compounds like copper hydroxide and copper sulfate basic and three rhizobacteria Burkholderia gladioli MRL408-3, Pseudomonas fluorescens TRH415-2 and Bacillus cereus KRY505-3 were selected to investigate their direct antibacterial effects using artificial media, aiming to identify effective pesticides against Xhc. Among them, treated medium with antibiotics such as streptomycin, oxolinic acid, and the antagonistic rhizobacteria MRL408-3 were formed inhibition zone. The agrochemicals and the rhizobacteria MRL408-3, which showed antibacterial effects on carrot leaves, pre-treated on the carrot leaves and then inoculated with Xhc. High control effects were shown on the carrot leaves pre-treated with both streptomycin and oxolinic acid. Scanning electron microscopy images of the carrot leaf surfaces showed that the population of bacteria decreased significantly on leaves pre-treated with streptomycin and oxolinic acid. From these results, it can be inferred that antibiotics like streptomycin and oxolinic acid exhibit superior control effects compared to other agents. This study provides valuable insights towards establishing an effective control system for bacterial blight of carrot.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139135576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-31DOI: 10.5423/rpd.2023.29.4.399
Sungho Woo, Yung Mi Lee, Dockyu Kim
Agricultural activities and the number of farms in the subarctic regions have been increasing annually after the coronavirus disease 2019 pandemic to achieve food self-sufficiency. Potatoes are vulnerable to soft rot bacteria at all stages of production, storage, and transportation. A novel bacterium, Pseudomonas sp. N3-W, isolated from Alaska tundra soil, grows at 5–25°C and produces extracellular protease(s). N3-W caused necrotic spots (hypersensitivity) in hot pepper leaves and soft rot disease (pathogenicity) in potato tubers. The psychrotolerant N3-W caused significant soft rot symptoms on potatoes at a broad temperature range (5°C, 15°C, and 25°C). In contrast, mesophilic Pectobacterium carotovorum KACC 16999 induced severe rotting symptoms in potatoes at their optimal growth temperature of 15°C and 25°C. However, it barely produced symptoms at 5°C, which is the appropriate storage and transportation temperature for potatoes. The results of pathogenicity testing imply that psychrotolerant soft rot pathogens from polar regions may cause severe soft rot not only during the crop growing season but also during storage and transportation. Our study indicates the possibility of new plant pathogen emergence and transmission due to the expansion of crop cultivation areas caused by permafrost thawing in response to recent polar warming.
{"title":"Potato Soft Rot Caused by Psychrotolerant Pseudomonas sp. from Subarctic Tundra Soil","authors":"Sungho Woo, Yung Mi Lee, Dockyu Kim","doi":"10.5423/rpd.2023.29.4.399","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.4.399","url":null,"abstract":"Agricultural activities and the number of farms in the subarctic regions have been increasing annually after the coronavirus disease 2019 pandemic to achieve food self-sufficiency. Potatoes are vulnerable to soft rot bacteria at all stages of production, storage, and transportation. A novel bacterium, Pseudomonas sp. N3-W, isolated from Alaska tundra soil, grows at 5–25°C and produces extracellular protease(s). N3-W caused necrotic spots (hypersensitivity) in hot pepper leaves and soft rot disease (pathogenicity) in potato tubers. The psychrotolerant N3-W caused significant soft rot symptoms on potatoes at a broad temperature range (5°C, 15°C, and 25°C). In contrast, mesophilic Pectobacterium carotovorum KACC 16999 induced severe rotting symptoms in potatoes at their optimal growth temperature of 15°C and 25°C. However, it barely produced symptoms at 5°C, which is the appropriate storage and transportation temperature for potatoes. The results of pathogenicity testing imply that psychrotolerant soft rot pathogens from polar regions may cause severe soft rot not only during the crop growing season but also during storage and transportation. Our study indicates the possibility of new plant pathogen emergence and transmission due to the expansion of crop cultivation areas caused by permafrost thawing in response to recent polar warming.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139131114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-31DOI: 10.5423/rpd.2023.29.4.331
Seon-Hee Kim, Jaehyuk Choi, Young-Joon Choi, Byeong-Yong Park, Su-Heon Lee, Gyoung Hee Kim, Hyun Gi Kong, Donggun Kim, Soonok Kim, Youngho Kim, C. Back, Hee-Seong Byun, Jang Kyun Seo, Jun Myoung Yu, Ju-Yeon Yoon, Dong-Hyeon Lee, Seung-Yeol Lee, Seungmo Lim, Yongho Jeon, Jaeyong Chun, Insoo Choi, Insoo Choi, Hyo-Won Choi, Jin Sung Hong, Seung-Beom Hong
More than a year has passed after the 6th edition of ‘List of Plant Diseases in Korea (LPDK)’ was published in April 2022. The 6.1st edition (2023) of List of Plant Diseases in Korea was made by correcting errors found in the 6th edition of list and adding new diseases reported after the 6th edition. There were 397 corrections from the 6th edition, most of which were simple spelling errors or minor issues. However, 12 diseases were deleted due to duplication or unclear literature proof, and 2 diseases had their diseases’ common names changed. We added 158 diseases that were reported before 2021 but not included in the 6th edition, or reported after the 6th edition. After all, 146 diseases were added to the 6,534 diseases in the 6th edition, resulting in a total of 6,680 diseases in the 6.1st edition. Thirty host taxa were also added, increasing the number from 1,390 in the 6th edition to 1,420 in the 6.1st edition. Pathogens were also added to 62 taxa, from 2,400 in the 6th edition, bringing the total to 2,462 taxa in the 6.1st edition. Ultimately, the 6.1st edition (2023) of ‘The List of Plant Diseases in Korea’ contains 6,680 diseases caused by pathogens of 2,462 taxa on 1,420 hosts. The 6.1st edition is not printed as a book, but is provided through the online ‘List of Plant Diseases in Korea’ (https://genebank.rda.go.kr/kplantdisease.do).
{"title":"Introduction of List of Plant Diseases in Korea 6.1st Edtion (2023 Revised Version)","authors":"Seon-Hee Kim, Jaehyuk Choi, Young-Joon Choi, Byeong-Yong Park, Su-Heon Lee, Gyoung Hee Kim, Hyun Gi Kong, Donggun Kim, Soonok Kim, Youngho Kim, C. Back, Hee-Seong Byun, Jang Kyun Seo, Jun Myoung Yu, Ju-Yeon Yoon, Dong-Hyeon Lee, Seung-Yeol Lee, Seungmo Lim, Yongho Jeon, Jaeyong Chun, Insoo Choi, Insoo Choi, Hyo-Won Choi, Jin Sung Hong, Seung-Beom Hong","doi":"10.5423/rpd.2023.29.4.331","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.4.331","url":null,"abstract":"More than a year has passed after the 6th edition of ‘List of Plant Diseases in Korea (LPDK)’ was published in April 2022. The 6.1st edition (2023) of List of Plant Diseases in Korea was made by correcting errors found in the 6th edition of list and adding new diseases reported after the 6th edition. There were 397 corrections from the 6th edition, most of which were simple spelling errors or minor issues. However, 12 diseases were deleted due to duplication or unclear literature proof, and 2 diseases had their diseases’ common names changed. We added 158 diseases that were reported before 2021 but not included in the 6th edition, or reported after the 6th edition. After all, 146 diseases were added to the 6,534 diseases in the 6th edition, resulting in a total of 6,680 diseases in the 6.1st edition. Thirty host taxa were also added, increasing the number from 1,390 in the 6th edition to 1,420 in the 6.1st edition. Pathogens were also added to 62 taxa, from 2,400 in the 6th edition, bringing the total to 2,462 taxa in the 6.1st edition. Ultimately, the 6.1st edition (2023) of ‘The List of Plant Diseases in Korea’ contains 6,680 diseases caused by pathogens of 2,462 taxa on 1,420 hosts. The 6.1st edition is not printed as a book, but is provided through the online ‘List of Plant Diseases in Korea’ (https://genebank.rda.go.kr/kplantdisease.do).","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139135105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-31DOI: 10.5423/rpd.2023.29.4.425
Gyoung Hee Kim, E. Choi
Kiwifruit industry has been threatened by the emergence of kiwifruit vine decline syndrome causing plant death within one or two years from symptom appearance. The main symptoms of this syndrome are root cortex breakdown, leaf necrosis, phylloptosis, fruit skin wrinkling, and twig wilting. Kiwifruit vine decline syndrome occurred on both Actinidia chinensis var. chinensis and A. chinensis var. deliciosa in mid-summer after rainy season. Kiwifruit vine decline syndrome was turned out to be severely occurred in wettable clay soils affected by waterlogging or poor aeration. No pathogens were directly correlated with the syndrome. Kiwifruit vine decline syndrome could be expected to be efficiently prevented controlled using Bounty 71 rootstock tolerant to water stress such as waterlogging.
{"title":"Occurrence of Kiwifruit Vine Decline Syndrome and Its Prevention Using Rootstock Tolerant to Waterlogging","authors":"Gyoung Hee Kim, E. Choi","doi":"10.5423/rpd.2023.29.4.425","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.4.425","url":null,"abstract":"Kiwifruit industry has been threatened by the emergence of kiwifruit vine decline syndrome causing plant death within one or two years from symptom appearance. The main symptoms of this syndrome are root cortex breakdown, leaf necrosis, phylloptosis, fruit skin wrinkling, and twig wilting. Kiwifruit vine decline syndrome occurred on both Actinidia chinensis var. chinensis and A. chinensis var. deliciosa in mid-summer after rainy season. Kiwifruit vine decline syndrome was turned out to be severely occurred in wettable clay soils affected by waterlogging or poor aeration. No pathogens were directly correlated with the syndrome. Kiwifruit vine decline syndrome could be expected to be efficiently prevented controlled using Bounty 71 rootstock tolerant to water stress such as waterlogging.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139135402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-31DOI: 10.5423/rpd.2023.29.4.414
Ji-Min Choi, Ji-Hye Song, Joonseob Ahn, Dea-Wook Kim, Kwang-Yeol Yang
In 2023, a number of seeds suspected to be discolored black oat seeds on oat farms in Gangjin were observed and examined for fungal infection. The species Alternaria alternata was predominant, accounting for 84% of all fungal infections. The appearance and quality of seeds harvested in 2022 were compared with seeds harvested in 2023 from the same field. The lightness value was lower in the seeds harvested in 2023, while the electrical conductivity was higher in the seeds harvested in 2023. The content of avenanthramide was found to be 10 times higher in the 2023 seeds than in those harvested in 2022. The accumulated precipitation in Gangjin in May 2023 was 230 times higher than that in May 2022, and the average relative humidity was high. These conditions created an environment suitable for infection of A. alternata, which were thought to have caused discolored black oat seeds.
{"title":"Detection of Alternaria alternata Associated with Discolored Black Oat Seeds in Korea","authors":"Ji-Min Choi, Ji-Hye Song, Joonseob Ahn, Dea-Wook Kim, Kwang-Yeol Yang","doi":"10.5423/rpd.2023.29.4.414","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.4.414","url":null,"abstract":"In 2023, a number of seeds suspected to be discolored black oat seeds on oat farms in Gangjin were observed and examined for fungal infection. The species Alternaria alternata was predominant, accounting for 84% of all fungal infections. The appearance and quality of seeds harvested in 2022 were compared with seeds harvested in 2023 from the same field. The lightness value was lower in the seeds harvested in 2023, while the electrical conductivity was higher in the seeds harvested in 2023. The content of avenanthramide was found to be 10 times higher in the 2023 seeds than in those harvested in 2022. The accumulated precipitation in Gangjin in May 2023 was 230 times higher than that in May 2022, and the average relative humidity was high. These conditions created an environment suitable for infection of A. alternata, which were thought to have caused discolored black oat seeds.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139135561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.316
Jun Woo Cho, Eunjung Roh, Yong Hwan Lee, Seong Hwan Kim
Recently, the domestic plant disease control policy for fire blight has been implemented partial control in addition to burial control. In this study, an on-site management survey was conducted targeting orchards that implemented partial disease control from 2019 to 2020 in order to find efficient implementation methods for partial disease control. As a result of an investigation into 22 pear and apple orchards in Cheonan and Chungju, 7 orchards were buried. The upper part of the cut infected plants was burned at 16 orchards and covered with plastic vinyl after lime treatment at 6 orchards. The lower stumps of cut infected plants were burned at 7 orchards and covered with plastic vinyl after lime treatment at 15 orchards. There were two orchards where suckers appeared on the stumps even though covers were applied. There was no infection by Erwinia amylovora in the suckers. The conservation condition of lime treatment was good, but warning signs were absent at 6 orchards. Most orchards treated the stumps and surrounding areas with glyphosate-isopropylamine herbicide. The effect of partial control was judged to be safe.
{"title":"Investigation on the Management Status of Pear and Apple Orchards Where Fire Blight Disease Was Partially Controlled in Korea","authors":"Jun Woo Cho, Eunjung Roh, Yong Hwan Lee, Seong Hwan Kim","doi":"10.5423/rpd.2023.29.3.316","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.316","url":null,"abstract":"Recently, the domestic plant disease control policy for fire blight has been implemented partial control in addition to burial control. In this study, an on-site management survey was conducted targeting orchards that implemented partial disease control from 2019 to 2020 in order to find efficient implementation methods for partial disease control. As a result of an investigation into 22 pear and apple orchards in Cheonan and Chungju, 7 orchards were buried. The upper part of the cut infected plants was burned at 16 orchards and covered with plastic vinyl after lime treatment at 6 orchards. The lower stumps of cut infected plants were burned at 7 orchards and covered with plastic vinyl after lime treatment at 15 orchards. There were two orchards where suckers appeared on the stumps even though covers were applied. There was no infection by Erwinia amylovora in the suckers. The conservation condition of lime treatment was good, but warning signs were absent at 6 orchards. Most orchards treated the stumps and surrounding areas with glyphosate-isopropylamine herbicide. The effect of partial control was judged to be safe.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.286
Seong Hyeon Yoon, Su Bin Lee, Eseul Baek, Ho-Jong Ju, Ju-Yeon Yoon
Biological and molecular characterization of a Korean isolate of Orthotospovirus chrysanthinecrocaulis (formerly known as chrysanthemum stem necrosis virus, CSNV) isolated from was determined using host range and sequence analysis in this study. Twenty-three species of indicator plants inoculated mechanically CSNV-Kr was investigated for determination of host range. CSNV-Kr induced various local and systemic symptoms in the inoculated plant species. CSNV-Kr could not infect three plant species and induced symptomless in systemic leaves in cultivars, though the plant samples reacted positively with the antiserum to CSNV by double-antibody sandwich–enzyme-linked immunosorbent assay. The complete genome sequence of CSNV-Kr was determined. The L RNA of CSNV-Kr consists of 8,959 nucleotides (nt) and encodes a putative RNA-dependent RNA polymerase. The M RNA of CSNV-Kr consists of 4,835 nt and encodes the movement protein (NSm) and the glycoprotein precursor (Gn/Gc protein). The S RNA of CNSV-Kr consists of 2,836 nt and encodes NSs protein and N protein. The Gn/Gc and N sequence of CSNV-Kr were compared with those of previously published CSNV isolates originating from different countries at nucleotide and amino acid levels. The Gn/GC sequence of CSNV-Kr shared 98.8−99.5% identity with CSNV isolated from other countries and the N sequence of CSNV-Kr shared 98.8−99.6% identity. No particular region of variability could be found in either grouping of viruses. All of the CSNV isolates did not show any relationship according to geographical origins and isolation hosts, suggesting no distinct segregation of the CSNV isolates.
{"title":"Biological and Molecular Characterization of a Korean Isolate of Orthotospovirus chrysanthinecrocaulis (Formerly Chrysanthemum Stem Necrosis Virus) Isolated from Chrysanthemum morifolium","authors":"Seong Hyeon Yoon, Su Bin Lee, Eseul Baek, Ho-Jong Ju, Ju-Yeon Yoon","doi":"10.5423/rpd.2023.29.3.286","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.286","url":null,"abstract":"Biological and molecular characterization of a Korean isolate of <i>Orthotospovirus chrysanthinecrocaulis</i> (formerly known as chrysanthemum stem necrosis virus, CSNV) isolated from <i.Chrysanthemum morifolium</i> was determined using host range and sequence analysis in this study. Twenty-three species of indicator plants inoculated mechanically CSNV-Kr was investigated for determination of host range. CSNV-Kr induced various local and systemic symptoms in the inoculated plant species. CSNV-Kr could not infect three plant species and induced symptomless in systemic leaves in <i.Nicotiana tabacum</i> cultivars, though the plant samples reacted positively with the antiserum to CSNV by double-antibody sandwich–enzyme-linked immunosorbent assay. The complete genome sequence of CSNV-Kr was determined. The L RNA of CSNV-Kr consists of 8,959 nucleotides (nt) and encodes a putative RNA-dependent RNA polymerase. The M RNA of CSNV-Kr consists of 4,835 nt and encodes the movement protein (NSm) and the glycoprotein precursor (Gn/Gc protein). The S RNA of CNSV-Kr consists of 2,836 nt and encodes NSs protein and N protein. The Gn/Gc and N sequence of CSNV-Kr were compared with those of previously published CSNV isolates originating from different countries at nucleotide and amino acid levels. The Gn/GC sequence of CSNV-Kr shared 98.8−99.5% identity with CSNV isolated from other countries and the N sequence of CSNV-Kr shared 98.8−99.6% identity. No particular region of variability could be found in either grouping of viruses. All of the CSNV isolates did not show any relationship according to geographical origins and isolation hosts, suggesting no distinct segregation of the CSNV isolates.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.304
Soo-Min Hong, Kyoung-Taek Park, Leonid N. Ten, Chang-Gi Back, In-Kyu Kang, Seung-Yeol Lee, Hee-Young Jung
Wilted and water-soaked lesion symptoms were observed on cucumbers in greenhouses located in Daejeon, Chungcheongnam-do, Korea, in June 2021. A bacterial strain, designated KNUB-04-21, was isolated from the cucumbers, which was subsequently identified as Pectobacterium brasiliense through a phylogenetic analysis based on sequences of the 16S rRNA region, dnaX, leuS, and recA genes. The biochemical characteristics of KNUB-04-21 were also similar to those of P. brasiliense through investigation using the API ID 32 GN system. The pathogenicity of KNUB-04-21 was confirmed by inoculating it into healthy cucumber plants. The reisolated strains were also found to be same to the original strain. To our knowledge, this is the first report of P. brasiliense being identified as the causative agent of cucumber soft rot in Korea.
2021年6月，忠清南道大田大棚的黄瓜出现了萎蔫和水浸的症状。从黄瓜中分离到一株菌株，命名为KNUB-04-21，随后鉴定为巴西乳杆菌。通过基于16S rRNA区域序列的系统发育分析，<i>dnaX, leuS</i>和<i>recA</i>基因。KNUB-04-21的生化特性也与<i>P相似。brasiliense< / i>通过调查使用API ID 32gn系统。将KNUB-04-21接种于健康黄瓜植株，证实其致病性。重新分离的菌株也发现与原菌株相同。据我们所知，这是第一次报告<i>P。brasiliense< / i>在韩国被鉴定为黄瓜软腐病的病原。
{"title":"First Report of Soft Rot Caused by Pectobacterium brasiliense on Cucumber in Korea","authors":"Soo-Min Hong, Kyoung-Taek Park, Leonid N. Ten, Chang-Gi Back, In-Kyu Kang, Seung-Yeol Lee, Hee-Young Jung","doi":"10.5423/rpd.2023.29.3.304","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.304","url":null,"abstract":"Wilted and water-soaked lesion symptoms were observed on cucumbers in greenhouses located in Daejeon, Chungcheongnam-do, Korea, in June 2021. A bacterial strain, designated KNUB-04-21, was isolated from the cucumbers, which was subsequently identified as <i>Pectobacterium brasiliense</i> through a phylogenetic analysis based on sequences of the 16S rRNA region, <i>dnaX, leuS</i>, and <i>recA</i> genes. The biochemical characteristics of KNUB-04-21 were also similar to those of <i>P. brasiliense</i> through investigation using the API ID 32 GN system. The pathogenicity of KNUB-04-21 was confirmed by inoculating it into healthy cucumber plants. The reisolated strains were also found to be same to the original strain. To our knowledge, this is the first report of <i>P. brasiliense</i> being identified as the causative agent of cucumber soft rot in Korea.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.276
Hong-Kyu Lee, Seongju Han, Sangmin Bak, Minseok Kim, Jean Geung Min, Hak ju Kim, Dong Hyun Kang, Minhui Kim, Wonyoung Jeong, Seungbin Baek, Minjoo Yang, Taegun Lim, Chanhoon An, Tae-Dong Kim, Chung Youl Park, Jae Sun Moon, Su-Heon Lee
This work investigated the viral infection in jujube plants in Korea. A total of 61 samples with the symptoms of putative viral infection were collected from experimental fields and orchards. Thereafter, the samples were subjected to metatranscriptome analysis, Reverse transcription polymerase chain reaction analysis, and nucleotide sequence analysis. These analyses identified the presence of two DNA viruses, jujube-associated badnavirus (JuBV), jujube mosaic-associated virus (JuMaV), and one RNA virus, jujube yellow mottle-associated virus (JYMaV). All samples collected were confirmed to be infected by at least one of the three viruses, with most showed multiple infections. The detection rates of JuBV, JYMaV, and JuMaV were 100%, 90.2%, and 8.2%, respectively. Only three combinations of viral infections were found: 9.8% of samples showed single infection of JuBV, 82.0% showed double infection of JuBV+JYMaV, and 8.2% showed triple infection of JuBV+JYMaV+JuMaV. Sequence analysis of the three viruses showed very high homology with respective virus isolates reported in China. This study is predicted to provide fundamental data to produce virus-free jujube seedlings and represents the first report of JuBV and JuMaV infection in Korea.
{"title":"Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea","authors":"Hong-Kyu Lee, Seongju Han, Sangmin Bak, Minseok Kim, Jean Geung Min, Hak ju Kim, Dong Hyun Kang, Minhui Kim, Wonyoung Jeong, Seungbin Baek, Minjoo Yang, Taegun Lim, Chanhoon An, Tae-Dong Kim, Chung Youl Park, Jae Sun Moon, Su-Heon Lee","doi":"10.5423/rpd.2023.29.3.276","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.276","url":null,"abstract":"This work investigated the viral infection in jujube plants in Korea. A total of 61 samples with the symptoms of putative viral infection were collected from experimental fields and orchards. Thereafter, the samples were subjected to metatranscriptome analysis, Reverse transcription polymerase chain reaction analysis, and nucleotide sequence analysis. These analyses identified the presence of two DNA viruses, jujube-associated badnavirus (JuBV), jujube mosaic-associated virus (JuMaV), and one RNA virus, jujube yellow mottle-associated virus (JYMaV). All samples collected were confirmed to be infected by at least one of the three viruses, with most showed multiple infections. The detection rates of JuBV, JYMaV, and JuMaV were 100%, 90.2%, and 8.2%, respectively. Only three combinations of viral infections were found: 9.8% of samples showed single infection of JuBV, 82.0% showed double infection of JuBV+JYMaV, and 8.2% showed triple infection of JuBV+JYMaV+JuMaV. Sequence analysis of the three viruses showed very high homology with respective virus isolates reported in China. This study is predicted to provide fundamental data to produce virus-free jujube seedlings and represents the first report of JuBV and JuMaV infection in Korea.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rust symptoms on Meliosma myriantha trees have been noticed during disease surveys in Korea since 2010, with a high disease incidence frequently surpassing 90%. The causal fungus of the rust disease was identified as Neophysopella vitis based on the morphological investigation and molecular sequence analysis of the internal transcribed spacer (ITS) and large subunit (LSU) rDNA regions. This is the first report of rust disease caused by N. vitis on M. myriantha in Korea. A pathogenicity assay proved that M. myriantha serves as the aecial host of N. vitis as spermogonia and aeciospores were produced, which can infect the two uredinial hosts, Boston ivy (Parthenocissus tricuspidata) and Virginia creeper (Parthenocissus quinquefolia).
{"title":"Identification and Pathogenicity of Neophysopella vitis Causing Rust Disease on Meliosma myriantha in Korea","authors":"Dong Hwan Na, Jae Sung Lee, Young-Joon Choi, Ji-Hyun Park, Hyeon-Dong Shin","doi":"10.5423/rpd.2023.29.3.299","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.299","url":null,"abstract":"Rust symptoms on <i>Meliosma myriantha</i> trees have been noticed during disease surveys in Korea since 2010, with a high disease incidence frequently surpassing 90%. The causal fungus of the rust disease was identified as <i>Neophysopella vitis</i> based on the morphological investigation and molecular sequence analysis of the internal transcribed spacer (ITS) and large subunit (LSU) rDNA regions. This is the first report of rust disease caused by <i>N. vitis</i> on <i>M. myriantha</i> in Korea. A pathogenicity assay proved that <i>M. myriantha</i> serves as the aecial host of <i>N. vitis</i> as spermogonia and aeciospores were produced, which can infect the two uredinial hosts, Boston ivy (<i>Parthenocissus tricuspidata</i>) and Virginia creeper (<i>Parthenocissus quinquefolia</i>).","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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