Pub Date : 2023-06-30DOI: 10.5423/rpd.2023.29.2.184
Wan-Gyu Kim, Gyo-Bin Lee, H. Shim, W. Cho
In May 2020, we surveyed disease occurrence on vegetables grown in Seosan area, Korea. During the disease survey, white rot symptoms were observed in Korean wild chive (Allium monanthum) plants growing in fields. The symptoms occurred mainly in the seed bulb-producing fields of the crop. The above ground parts of the diseased plants displayed premature yellowing and dying of older leaves and stunting of the plants. The bulbs and roots of the diseased plants turned black and rotted. The disease occurred in a range of 1–60% in four of the eight fields surveyed. Three isolates of Sclerotium sp. were obtained from the bulb lesions of diseased plants. All isolates were identified as Stromatinia cepivora based on the morphological characteristics and phylogenetic analysis. Pathogenicity of the isolates on Korean wild chive was confirmed by artificial inoculation test. The lesions induced by the inoculation test were similar to those observed in the investigated fields. This is the first report of S. cepivora causing white rot in Korean wild chive.
{"title":"White Rot of Korean Wild Chive Caused by Stromatinia cepivora","authors":"Wan-Gyu Kim, Gyo-Bin Lee, H. Shim, W. Cho","doi":"10.5423/rpd.2023.29.2.184","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.2.184","url":null,"abstract":"In May 2020, we surveyed disease occurrence on vegetables grown in Seosan area, Korea. During the disease survey, white rot symptoms were observed in Korean wild chive (Allium monanthum) plants growing in fields. The symptoms occurred mainly in the seed bulb-producing fields of the crop. The above ground parts of the diseased plants displayed premature yellowing and dying of older leaves and stunting of the plants. The bulbs and roots of the diseased plants turned black and rotted. The disease occurred in a range of 1–60% in four of the eight fields surveyed. Three isolates of Sclerotium sp. were obtained from the bulb lesions of diseased plants. All isolates were identified as Stromatinia cepivora based on the morphological characteristics and phylogenetic analysis. Pathogenicity of the isolates on Korean wild chive was confirmed by artificial inoculation test. The lesions induced by the inoculation test were similar to those observed in the investigated fields. This is the first report of S. cepivora causing white rot in Korean wild chive.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43594695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.5423/rpd.2023.29.2.200
HyeRan Moon, S. Yoo, M. Sang
Climate change has occurred frequently, and it lead to a severe decrease in marketability and productivity. Onion has various functional elements related to antioxidant, antibacterial, anticancer and immune enhancement, the productivity is closely affected by climate conditions. In this study, to environmentally-friendly produce onions even though drought condition, effect of Bacillus aryabhattai H19-1 on the yield, marketability, and functional component of onions was evaluated in a field. When strain H19-1 was drenched, onion production and marketability significantly increased, and the contents of ascorbic acid and carotenoid of harvested onion bulbs also increased. Therefore, B. aryabhattai H19-1 is expected to be effective in enhancing onion productivity by mitigating drought stress.
{"title":"Bacillus aryabhattai H19-1-Mediated Mitigation of Drought Stress in Onion Bulb Development and Maturation","authors":"HyeRan Moon, S. Yoo, M. Sang","doi":"10.5423/rpd.2023.29.2.200","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.2.200","url":null,"abstract":"Climate change has occurred frequently, and it lead to a severe decrease in marketability and productivity. Onion has various functional elements related to antioxidant, antibacterial, anticancer and immune enhancement, the productivity is closely affected by climate conditions. In this study, to environmentally-friendly produce onions even though drought condition, effect of Bacillus aryabhattai H19-1 on the yield, marketability, and functional component of onions was evaluated in a field. When strain H19-1 was drenched, onion production and marketability significantly increased, and the contents of ascorbic acid and carotenoid of harvested onion bulbs also increased. Therefore, B. aryabhattai H19-1 is expected to be effective in enhancing onion productivity by mitigating drought stress.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41855927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.5423/rpd.2023.29.2.193
Nam‐Yeon Kim, R. Jeong
The apple stem grooving virus (ASGV) is one of the most harmful latent viruses infecting pear orchards worldwide. To examine the genetic diversity of ASGV in Korean pear orchards, the complete coat protein (CP) gene of five ASGV isolates collected from various regions were identified. The five Korean ASGV isolates showed 88–96% nucleotide identity with the 11 isolates worldwide occurring elsewhere in the world. Phylogenetic analysis of five isolates, as well as the previously sequenced isolates, indicated that the ASGV clusters had no correlation with the host or geographical regions of origin. Recombination analysis showed that one of the five Korean isolates is a recombinant, with a recombination site in the CP gene region (nt 532–708). This study is the first report of natural recombination within the CP gene of ASGV isolates from pears grown in Korea.
{"title":"Phylogenetic and Recombination Analysis of Apple Stem Grooving Virus Isolates from Pears in Korea","authors":"Nam‐Yeon Kim, R. Jeong","doi":"10.5423/rpd.2023.29.2.193","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.2.193","url":null,"abstract":"The apple stem grooving virus (ASGV) is one of the most harmful latent viruses infecting pear orchards worldwide. To examine the genetic diversity of ASGV in Korean pear orchards, the complete coat protein (CP) gene of five ASGV isolates collected from various regions were identified. The five Korean ASGV isolates showed 88–96% nucleotide identity with the 11 isolates worldwide occurring elsewhere in the world. Phylogenetic analysis of five isolates, as well as the previously sequenced isolates, indicated that the ASGV clusters had no correlation with the host or geographical regions of origin. Recombination analysis showed that one of the five Korean isolates is a recombinant, with a recombination site in the CP gene region (nt 532–708). This study is the first report of natural recombination within the CP gene of ASGV isolates from pears grown in Korea.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43967563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.5423/rpd.2023.29.2.101
Eu Ddeum Choi, Janghoon Song, Ho-Jin Seo
Pear scab, caused by Venturia nashicola, is one of the most devastating diseases of Asian pears in Korea, Japan, China, and Taiwan. To manage this disease, growers mainly relied on chemical control. However, continuous use of chemical causes not only environmental contaminant but also the emergence of resistance to pathogens, so a more sustainable management plan is needed. Therefore, it is necessary to understand the life cycle and infection characteristics of V. nashicola and to set an active control strategy according to meteorological conditions rather than, as in the past, calendar-based control or continuous use of a specific fungicide system. Various results of the related research results were reviewed to summarize the race, infection characteristics, and control system of V. nashicola, a pear scab, and to discuss plans for a more effective control system.
{"title":"Characteristics and Control of Pear Scab (Venturia nashicola): A Review","authors":"Eu Ddeum Choi, Janghoon Song, Ho-Jin Seo","doi":"10.5423/rpd.2023.29.2.101","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.2.101","url":null,"abstract":"Pear scab, caused by <i>Venturia nashicola</i>, is one of the most devastating diseases of Asian pears in Korea, Japan, China, and Taiwan. To manage this disease, growers mainly relied on chemical control. However, continuous use of chemical causes not only environmental contaminant but also the emergence of resistance to pathogens, so a more sustainable management plan is needed. Therefore, it is necessary to understand the life cycle and infection characteristics of <i>V. nashicola</i> and to set an active control strategy according to meteorological conditions rather than, as in the past, calendar-based control or continuous use of a specific fungicide system. Various results of the related research results were reviewed to summarize the race, infection characteristics, and control system of <i>V. nashicola</i>, a pear scab, and to discuss plans for a more effective control system.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136064790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.5423/rpd.2023.29.2.188
Jiwon Kim, M. Sang
Abiotic and biotic stresses have been a serious threat to crop growth and productivity in the agricultural system. In this study, four strains (HS1, H30-3, KJ40, and BC42), which have biological activities related to disease suppression or alleviation of salinity and drought stresses, were tested for broad-spectrum biocontrol activity against anthracnose caused by Colletotrichum orbiculare, a bacterial fruit blotch caused by Acidovorax citrulli, and Fusarium wilt caused by Fusarium oxysporum in cucumber plants. As a result of test, when the four strains were drenched into the soil, anthracnose in cucumber leaves significantly decrease; strain KJ40 suppressed disease incidence by A. citrulli; strain BC42 significantly reduced bacterial fruit blotch and Fusarium wilt compared to control. Therefore, strain KJ40 could be a biocontrol candidate for controlling anthracnose through induced systemic resistance and the disease caused by A. citrulli as well as alleviating drought stress; strain BC42 has broad-spectrum biocontrol activity against anthracnose, Fusarium wilt, and bacterial fruit blotch.
{"title":"Biocontrol Activities of Peribacillus butanolivorans KJ40, Bacillus zanthoxyli HS1, B. siamensis H30-3 and Pseudomonas sp. BC42 on Anthracnose, Bacterial Fruit Blotch and Fusarium Wilt of Cucumber Plants","authors":"Jiwon Kim, M. Sang","doi":"10.5423/rpd.2023.29.2.188","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.2.188","url":null,"abstract":"Abiotic and biotic stresses have been a serious threat to crop growth and productivity in the agricultural system. In this study, four strains (HS1, H30-3, KJ40, and BC42), which have biological activities related to disease suppression or alleviation of salinity and drought stresses, were tested for broad-spectrum biocontrol activity against anthracnose caused by Colletotrichum orbiculare, a bacterial fruit blotch caused by Acidovorax citrulli, and Fusarium wilt caused by Fusarium oxysporum in cucumber plants. As a result of test, when the four strains were drenched into the soil, anthracnose in cucumber leaves significantly decrease; strain KJ40 suppressed disease incidence by A. citrulli; strain BC42 significantly reduced bacterial fruit blotch and Fusarium wilt compared to control. Therefore, strain KJ40 could be a biocontrol candidate for controlling anthracnose through induced systemic resistance and the disease caused by A. citrulli as well as alleviating drought stress; strain BC42 has broad-spectrum biocontrol activity against anthracnose, Fusarium wilt, and bacterial fruit blotch.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46506477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.5423/rpd.2023.29.2.174
Da-Ran Kim, Youn-Sig Kwak
The limited effectiveness of current plant disease management treatments necessitates the development of new methods for controlling diseases using beneficial microbes. Demanding sustainable agriculture is increasingly highlighted as a biocontrol approach, particularly Streptomyces species known to produce a variety of antibiotic compounds and secondary metabolites. The Streptomyces globisporus SP6C4 strain and Streptomyces sp. S8 have been reported as potent antifungal agents and are gaining attention for improving crop growth in sustainable agriculture. In this study, we investigated the use of Streptomyces species formulations to enhance bacterial growth with nitrogen sources. Specifically, the addition of L-glutamic acid and L-cysteine resulted in earlier sporulation and bacterial growth in Streptomyces strains, respectively. This approach could expand the range of fermentation techniques in agriculture and be useful for controlling plant growth-promoting bacteria.
{"title":"Optimization of Culture and Sporulation for Two Plant Beneficial Streptomyces Strains","authors":"Da-Ran Kim, Youn-Sig Kwak","doi":"10.5423/rpd.2023.29.2.174","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.2.174","url":null,"abstract":"The limited effectiveness of current plant disease management treatments necessitates the development of new methods for controlling diseases using beneficial microbes. Demanding sustainable agriculture is increasingly highlighted as a biocontrol approach, particularly Streptomyces species known to produce a variety of antibiotic compounds and secondary metabolites. The Streptomyces globisporus SP6C4 strain and Streptomyces sp. S8 have been reported as potent antifungal agents and are gaining attention for improving crop growth in sustainable agriculture. In this study, we investigated the use of Streptomyces species formulations to enhance bacterial growth with nitrogen sources. Specifically, the addition of L-glutamic acid and L-cysteine resulted in earlier sporulation and bacterial growth in Streptomyces strains, respectively. This approach could expand the range of fermentation techniques in agriculture and be useful for controlling plant growth-promoting bacteria.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46529917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.5423/rpd.2023.29.2.168
Ji-Yeon Kim, K. Baek, Sun-Young Lee
Agricultural antibiotics are widely used to inhibit the growth of phytopathogenic bacteria involved in plant diseases. However, continuous antibiotic overuse in crop production may lead to the development of antibiotic resistance in phytopathogenic bacteria. This study was conducted to evaluate the resistance to three different agricultural antibiotics (oxytetracycline+streptomycin, streptomycin, and validamycin A) in 91 strains of phytopathogenic bacteria including Pectobacterium carotovorum, Pseudomonas syringae pv. actinidiae, Clavibacter michiganensis subsp. michiganensis, C. michiganensis subsp. capsici, and Xanthomonas arboricola pv. pruni. Bacterial growth in the presence of various concentrations of validamycin A was also assessed spectrophotometrically by analyzing the optical density. All strains did not grow when the cells were exposed to oxytetracycline+streptomycin or 100× of streptomycin. However, among the 91 strains, 4% and 2% strains showed bacterial growth at the concentrations of 1× and 10× of streptomycin, respectively. Furthermore, 97%, 93%, and 73% strains were resistant to the 1×, 10×, and 100× of validamycin A, respectively, and especially, P. carotovorum contained the highest resistance to the validamycin A. Minimum bactericidal concentration values of validamycin A did not correlate with the patterns of agricultural antibiotic resistance. Further studies are needed to understand the incidence and development of antibiotic resistance in phytopathogenic bacteria.
{"title":"Evaluation of Resistance of Phytopathogenic Bacteria to Agricultural Antibiotics","authors":"Ji-Yeon Kim, K. Baek, Sun-Young Lee","doi":"10.5423/rpd.2023.29.2.168","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.2.168","url":null,"abstract":"Agricultural antibiotics are widely used to inhibit the growth of phytopathogenic bacteria involved in plant diseases. However, continuous antibiotic overuse in crop production may lead to the development of antibiotic resistance in phytopathogenic bacteria. This study was conducted to evaluate the resistance to three different agricultural antibiotics (oxytetracycline+streptomycin, streptomycin, and validamycin A) in 91 strains of phytopathogenic bacteria including Pectobacterium carotovorum, Pseudomonas syringae pv. actinidiae, Clavibacter michiganensis subsp. michiganensis, C. michiganensis subsp. capsici, and Xanthomonas arboricola pv. pruni. Bacterial growth in the presence of various concentrations of validamycin A was also assessed spectrophotometrically by analyzing the optical density. All strains did not grow when the cells were exposed to oxytetracycline+streptomycin or 100× of streptomycin. However, among the 91 strains, 4% and 2% strains showed bacterial growth at the concentrations of 1× and 10× of streptomycin, respectively. Furthermore, 97%, 93%, and 73% strains were resistant to the 1×, 10×, and 100× of validamycin A, respectively, and especially, P. carotovorum contained the highest resistance to the validamycin A. Minimum bactericidal concentration values of validamycin A did not correlate with the patterns of agricultural antibiotic resistance. Further studies are needed to understand the incidence and development of antibiotic resistance in phytopathogenic bacteria.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46844984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.5423/rpd.2023.29.2.137
Mxolisi Mtyobile, Silindile Miya
Exserohilum turcicum is a fungus that causes northern corn leaf blight (NCLB) and has deleterious effects on maize production globally. Therefore, it is prudent to mitigate the effects of NCLB using genetic diversity. The objective of this research was to assess the severity of NCLB disease on the growth and yield of various maize genotypes. A randomized complete block design field experiment, replicated three times, was conducted to evaluate the effect of E. turcicum on 10 maize hybrids. Percent disease index, plant height, and leaf area were recorded at the silk stage. Cob weight, grain fresh weight, and grain yields were determined at harvest maturity. All measured parameters were significantly different (P<0.05) between the maize hybrids. Of the 10 genotypes, four (PAN 4R-528R, PAN 4R-728BR, PAN 3R-724BR, and P1788BR) were susceptible, five (DKC74-74BR, PAN 5R-582R, PAN 5R-890R, PAN 5R-854R, and PAN 5R-590R) were moderately susceptible, and one (DKC80-40BR) was moderately resistant. DKC80-40BR exhibited greater cob weight, while DKC74-74BR was superior in all other plant growth and yield components. Interestingly, although not significant (P>0.05) and high, maize growth and yield parameters had negative correlations with disease incidence, except for grain fresh weight. Therefore, DKC80-40BR may be selected for cultivation in areas prone to NCLB to reduce maize susceptibility to the disease, while DKC74-74BR may improve crop performance. These hybrids could be considered as potential sources of resistance or tolerance to NCLB for further validation by plant breeders.
{"title":"Exserohilum turcicum (Northern Corn Leaf Blight) Severity on Maize Hybrids and the Associated Crop Performance in O.R. Tambo District, Eastern Cape, South Africa","authors":"Mxolisi Mtyobile, Silindile Miya","doi":"10.5423/rpd.2023.29.2.137","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.2.137","url":null,"abstract":"Exserohilum turcicum is a fungus that causes northern corn leaf blight (NCLB) and has deleterious effects on maize production globally. Therefore, it is prudent to mitigate the effects of NCLB using genetic diversity. The objective of this research was to assess the severity of NCLB disease on the growth and yield of various maize genotypes. A randomized complete block design field experiment, replicated three times, was conducted to evaluate the effect of E. turcicum on 10 maize hybrids. Percent disease index, plant height, and leaf area were recorded at the silk stage. Cob weight, grain fresh weight, and grain yields were determined at harvest maturity. All measured parameters were significantly different (P<0.05) between the maize hybrids. Of the 10 genotypes, four (PAN 4R-528R, PAN 4R-728BR, PAN 3R-724BR, and P1788BR) were susceptible, five (DKC74-74BR, PAN 5R-582R, PAN 5R-890R, PAN 5R-854R, and PAN 5R-590R) were moderately susceptible, and one (DKC80-40BR) was moderately resistant. DKC80-40BR exhibited greater cob weight, while DKC74-74BR was superior in all other plant growth and yield components. Interestingly, although not significant (P>0.05) and high, maize growth and yield parameters had negative correlations with disease incidence, except for grain fresh weight. Therefore, DKC80-40BR may be selected for cultivation in areas prone to NCLB to reduce maize susceptibility to the disease, while DKC74-74BR may improve crop performance. These hybrids could be considered as potential sources of resistance or tolerance to NCLB for further validation by plant breeders.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45103167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.5423/rpd.2023.29.2.108
Myung-Hwi Kim, Seok-Yeong Jang, Ji-soo Choi, H. Byun, H. Kwak, Su-Heon Lee, Jang-Kyun Seo
With the development of advanced virus diagnostic technologies, numerous viruses, including novel viruses, have been identified explosively from various biological samples around the world over the last decade. For plant viruses, approximately 376 novel viruses have been reported in the last three years. Information on the occurrence and host ranges of plant viral diseases in a particular country or region is very important for diagnosis, quarantine, and control of the viral diseases. Recently, based on active research on the diagnosis and identification of plant viruses, a significant number of newly occurring viruses and new viral host plants have been reported in South Korea. This review paper provides integrated information on plant viruses and viral host plants newly reported in South Korea between 2020 and 2022 to help diagnose, control, and quarantine plant virus diseases in crop fields.
{"title":"Plant Viruses and Viral Host Plants Newly Reported in South Korea in 2020–2022","authors":"Myung-Hwi Kim, Seok-Yeong Jang, Ji-soo Choi, H. Byun, H. Kwak, Su-Heon Lee, Jang-Kyun Seo","doi":"10.5423/rpd.2023.29.2.108","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.2.108","url":null,"abstract":"With the development of advanced virus diagnostic technologies, numerous viruses, including novel viruses, have been identified explosively from various biological samples around the world over the last decade. For plant viruses, approximately 376 novel viruses have been reported in the last three years. Information on the occurrence and host ranges of plant viral diseases in a particular country or region is very important for diagnosis, quarantine, and control of the viral diseases. Recently, based on active research on the diagnosis and identification of plant viruses, a significant number of newly occurring viruses and new viral host plants have been reported in South Korea. This review paper provides integrated information on plant viruses and viral host plants newly reported in South Korea between 2020 and 2022 to help diagnose, control, and quarantine plant virus diseases in crop fields.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45153097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.5423/rpd.2023.29.2.130
Zar Zar Soe, Y. Shin, Hyun Su Kang, Mi Jin Kim, Y. Jeun
Citrus melanoses caused by Diaporthe citri, has been one of the serious diseases in many citrus orchards of Jeju Island. To protect melanose in citrus farms, a fast and exact diagnosis method is necessary. In this study, diseased leaves and dieback twigs were collected from a total of 49 farms within March to April in 2022. A total of 465 fungal isolates were obtained from a total of 358 isolated plant samples. Among these fungal isolates, 40 representatives of D. citrii isolates which were isolated from 22 twigs and 18 leaves on 23 farms were found based on cultural characteristics on potato dextrose agar and conidial morphology. Additionally, the molecular assay was carried out and compared with those by morphological diagnosis. All isolates were identified as D. citrii by analyzing the sequences at the internal transcribed spacer (ITS) rDNA region using primers of ITS1/ITS4 or at β-tubulin using primer Btdcitri-F/R. Therefore, based on the present study, where the results of morphological identification of conidial type were consistent with DNA sequence analysis of certain gene, choosing a suitable method for a fast diagnosis of citrus melanose was suggested.
{"title":"Rapid Identification of Diaporthe citri by Gene Sequence Analysis","authors":"Zar Zar Soe, Y. Shin, Hyun Su Kang, Mi Jin Kim, Y. Jeun","doi":"10.5423/rpd.2023.29.2.130","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.2.130","url":null,"abstract":"Citrus melanoses caused by Diaporthe citri, has been one of the serious diseases in many citrus orchards of Jeju Island. To protect melanose in citrus farms, a fast and exact diagnosis method is necessary. In this study, diseased leaves and dieback twigs were collected from a total of 49 farms within March to April in 2022. A total of 465 fungal isolates were obtained from a total of 358 isolated plant samples. Among these fungal isolates, 40 representatives of D. citrii isolates which were isolated from 22 twigs and 18 leaves on 23 farms were found based on cultural characteristics on potato dextrose agar and conidial morphology. Additionally, the molecular assay was carried out and compared with those by morphological diagnosis. All isolates were identified as D. citrii by analyzing the sequences at the internal transcribed spacer (ITS) rDNA region using primers of ITS1/ITS4 or at β-tubulin using primer Btdcitri-F/R. Therefore, based on the present study, where the results of morphological identification of conidial type were consistent with DNA sequence analysis of certain gene, choosing a suitable method for a fast diagnosis of citrus melanose was suggested.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47792455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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