Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.251
Sena Yoon, Jiyoung Min, Heung Tae Kim
In order to examine the pathogenicity of Alternaria dauci, the causal agent of carrot leaf blight, it is necessary to standardize sporulation conditions to ensure the optimal quantity and quality of spore inoculum. Therefore, in this study, the effects of the growth medium, aeration treatment, and UV treatment with 12-hr photoperiod on the sporulation of A. dauci KACC42997 were investigated. A. dauci KACC42997 was pre-cultured for 7 days in a potato dextrose agar medium at 25oC in the dark condition. When the pre-culture, after removing aerial mycelia, was re-incubated for 5 days, with simultaneous aeration treatment and 12-hr cycle UV treatment at 20oC, the largest number of spores was produced. One hundred seventy isolates of A. dauci were isolated from major carrot growing regions such as Pyeongchang, Gumi and Jeju and tested for sporulation under the conditions established in this study. Except for 20 strains, all strains produced spores. Statistically significant differences in the extent of sporulation were found among local populations of A. dauci isolates, but no difference was observed in their pathogenicity on carrots.
{"title":"Factors Affecting Spore Formation of Carrot Leaf Blight Caused by Alternaria dauci In Vitro","authors":"Sena Yoon, Jiyoung Min, Heung Tae Kim","doi":"10.5423/rpd.2023.29.3.251","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.251","url":null,"abstract":"In order to examine the pathogenicity of <i>Alternaria dauci</i>, the causal agent of carrot leaf blight, it is necessary to standardize sporulation conditions to ensure the optimal quantity and quality of spore inoculum. Therefore, in this study, the effects of the growth medium, aeration treatment, and UV treatment with 12-hr photoperiod on the sporulation of <i>A. dauci</i> KACC42997 were investigated. <i>A. dauci</i> KACC42997 was pre-cultured for 7 days in a potato dextrose agar medium at 25oC in the dark condition. When the pre-culture, after removing aerial mycelia, was re-incubated for 5 days, with simultaneous aeration treatment and 12-hr cycle UV treatment at 20oC, the largest number of spores was produced. One hundred seventy isolates of <i>A. dauci</i> were isolated from major carrot growing regions such as Pyeongchang, Gumi and Jeju and tested for sporulation under the conditions established in this study. Except for 20 strains, all strains produced spores. Statistically significant differences in the extent of sporulation were found among local populations of <i>A. dauci</i> isolates, but no difference was observed in their pathogenicity on carrots.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.321
Lingmin Jiang, Ho Le Han, Yuxin Peng, Doeun Jeon, Donghyun Cho, Cha Young Kim, Jiyoung Lee
The bacterium Neobacillus endophyticus BRMEA1T, isolated from the medicinal plant Selaginella involvens, known as its thermotolerant can grow at 50°C. To explore the genetic basis for its heat tolerance response and its potential for producing valuable natural compounds, the genomes of two thermotolerant and four mesophilic strains in the genus Neobacillus were analyzed using a bioinformatic software platform. The whole genome was annotated using RAST SEED and OrthVenn2, with a focus on identifying potential heattolerance- related genes. N. endophyticus BRMEA1T was found to possess more stress response genes compared to other mesophilic members of the genus, and it was the only strain that had genes for the synthesis of osmoregulated periplasmic glucans. This study sheds light on the potential value of N. endophyticus BRMEA1T, as it reveals the mechanism of heat resistance and the application of secondary metabolites produced by this bacterium through whole-genome sequencing and comparative analysis.
{"title":"Genome-Based Insights into the Thermotolerant Adaptations of Neobacillus endophyticus BRMEA1T","authors":"Lingmin Jiang, Ho Le Han, Yuxin Peng, Doeun Jeon, Donghyun Cho, Cha Young Kim, Jiyoung Lee","doi":"10.5423/rpd.2023.29.3.321","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.321","url":null,"abstract":"The bacterium <i>Neobacillus endophyticus</i> BRMEA1<sup>T</sup>, isolated from the medicinal plant <i>Selaginella involvens</i>, known as its thermotolerant can grow at 50°C. To explore the genetic basis for its heat tolerance response and its potential for producing valuable natural compounds, the genomes of two thermotolerant and four mesophilic strains in the genus <i>Neobacillus</i> were analyzed using a bioinformatic software platform. The whole genome was annotated using RAST SEED and OrthVenn2, with a focus on identifying potential heattolerance- related genes. <i>N. endophyticus</i> BRMEA1<sup>T</sup> was found to possess more stress response genes compared to other mesophilic members of the genus, and it was the only strain that had genes for the synthesis of osmoregulated periplasmic glucans. This study sheds light on the potential value of <i>N. endophyticus</i> BRMEA1<sup>T</sup>, as it reveals the mechanism of heat resistance and the application of secondary metabolites produced by this bacterium through whole-genome sequencing and comparative analysis.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.220
Pandiyan Muthuramalingam, Rajendran Jeyasri, Yeonju Park, Seongho Lee, Jae Hoon Jeong, Yunji Shin, Jinwook Kim, Sangmin Jung, Hyunsuk Shin
The phenomena of global warming has led to an increase in the average air temperature in temperate climates. Springtime frost damage is becoming more common, and after a period of dormancy, damage to buds, blooms, and developing fruits is greater significant than damage from low winter temperatures. Peaches are a crucial crop among moderate fruits. Spring frost damage in peaches can have a negative effect on crop growth, yield, and quality. It is noteworthy that these plants have evolved defenses against spring frost damage while being exposed to a variety of low temperatures in the early spring. In this current review, recent research advancements on spring frost damage avoidance in peaches were deliberated. Additionally, adaptive mechanisms of peach, such as deacclimation and reacclimation, were emphasized. Moreover, the emerging advancements using various omics approaches revealed the peach physiology and molecular mechanisms comprehensively. Furthermore, the use of chemical products and understanding the spring frost mechanisms through the use of environmental chamber temperature stimulation and infrared thermography studies were also discussed. This review is essential groundwork and paves the way to derive and design future research for agronomists and horticulturalists to overcome the challenges of spring frost damage avoidance and crop management in these circumstances.
{"title":"Emerging Research Advancements to Overcome the Peach Spring Frost","authors":"Pandiyan Muthuramalingam, Rajendran Jeyasri, Yeonju Park, Seongho Lee, Jae Hoon Jeong, Yunji Shin, Jinwook Kim, Sangmin Jung, Hyunsuk Shin","doi":"10.5423/rpd.2023.29.3.220","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.220","url":null,"abstract":"The phenomena of global warming has led to an increase in the average air temperature in temperate climates. Springtime frost damage is becoming more common, and after a period of dormancy, damage to buds, blooms, and developing fruits is greater significant than damage from low winter temperatures. Peaches are a crucial crop among moderate fruits. Spring frost damage in peaches can have a negative effect on crop growth, yield, and quality. It is noteworthy that these plants have evolved defenses against spring frost damage while being exposed to a variety of low temperatures in the early spring. In this current review, recent research advancements on spring frost damage avoidance in peaches were deliberated. Additionally, adaptive mechanisms of peach, such as deacclimation and reacclimation, were emphasized. Moreover, the emerging advancements using various omics approaches revealed the peach physiology and molecular mechanisms comprehensively. Furthermore, the use of chemical products and understanding the spring frost mechanisms through the use of environmental chamber temperature stimulation and infrared thermography studies were also discussed. This review is essential groundwork and paves the way to derive and design future research for agronomists and horticulturalists to overcome the challenges of spring frost damage avoidance and crop management in these circumstances.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.258
Soo Min Lee, Eun Ju Jo, Hun Kim, Gyung Ja Choi
Fusarium wilt, caused by Fusarium oxysporum f. sp. niveum (Fon) is a serious disease in watermelon cultivation. Most of commercialized watermelon cultivars to Fusarium wilt are susceptible in Korea. Fon isolates were divided into four races (races 0, 1, 2, and 3), based on pathogenicity in four watermelon differentials including ‘Sugar baby’, ‘Charleston gray’, ‘Calhoun gray’ and ‘PI-296341-FR’. We obtained 7 isolates of Fon and tested to determine race of the fungal strains. Fon KACC 40902 and Fon HA were race 0 and Fon NW1, Fon NW2, Fon CW and Fon KACC 40901 were race 1. And Fon KACC 40905 was race 2, but race 3 isolate of Fon was not founded. We also tested virulence of seven Fon isolates on three-susceptible cultivars of watermelon. The isolates showed different virulence on the cultivars. In addition, to study the resistance characteristics of watermelon to Fon, we selected three moderately or highly resistant cultivars of watermelon and occurrence of Fusarium wilt in seedlings of the cultivars by seven Fon isolates was investigated. Among them, ‘Calhoun gray’ is highly resistant to six Fon isolates except Fon KACC 40905. On the other two cultivars, disease severity of Fusarium wilt caused by each isolate was positively correlated with the virulence of the Fon isolates. The results suggest that resistance of the watermelon cultivars to Fon isolates is likely affected by the virulence of the pathogen.
{"title":"Resistance Characteristics of Watermelon Cultivars to Fusarium oxysporum f. sp. niveum","authors":"Soo Min Lee, Eun Ju Jo, Hun Kim, Gyung Ja Choi","doi":"10.5423/rpd.2023.29.3.258","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.258","url":null,"abstract":"Fusarium wilt, caused by <i>Fusarium oxysporum</i> f. sp. <i>niveum</i> (Fon) is a serious disease in watermelon cultivation. Most of commercialized watermelon cultivars to Fusarium wilt are susceptible in Korea. Fon isolates were divided into four races (races 0, 1, 2, and 3), based on pathogenicity in four watermelon differentials including ‘Sugar baby’, ‘Charleston gray’, ‘Calhoun gray’ and ‘PI-296341-<i>FR</i>’. We obtained 7 isolates of Fon and tested to determine race of the fungal strains. Fon KACC 40902 and Fon HA were race 0 and Fon NW1, Fon NW2, Fon CW and Fon KACC 40901 were race 1. And Fon KACC 40905 was race 2, but race 3 isolate of Fon was not founded. We also tested virulence of seven Fon isolates on three-susceptible cultivars of watermelon. The isolates showed different virulence on the cultivars. In addition, to study the resistance characteristics of watermelon to Fon, we selected three moderately or highly resistant cultivars of watermelon and occurrence of Fusarium wilt in seedlings of the cultivars by seven Fon isolates was investigated. Among them, ‘Calhoun gray’ is highly resistant to six Fon isolates except Fon KACC 40905. On the other two cultivars, disease severity of Fusarium wilt caused by each isolate was positively correlated with the virulence of the Fon isolates. The results suggest that resistance of the watermelon cultivars to Fon isolates is likely affected by the virulence of the pathogen.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.234
Ji Hye Woo, Mikyeong Kim, Kun-Woo Lee, Sang-sup Han, Byeongjin Cha
In early spring, water-soaked lesions appeared on the petals and leaves of gaenari (Forsythia koreana), and the tissues were necrotic and dry. Cankers appeared on the infected branches around late spring and the above part of a branch withered and died. However, it was very rare that the base of the cankered-branch died. The identical fungi were isolated from the lesions on various tissues, and they grew with white colonies on potato dextrose agar medium. The fungus grew most actively at 23oC and produced many sclerotia of various sizes. In a pathogenicity assay in which mycelial and sclerotial suspensions were inoculated on each organ of forsythia, it was found that the pathogen infects the flower only, but not the leaves or branches. Symptoms on the flowers spread to the next leaves and branches over time and the infected branches were eventually withered. To identify the isolates, DNA sequences of four phylogenetic markers including ITS, LSU, Tub2, and CAL were analyzed and all isolates were identified as a species in the genus Septotinia. This is not only the first report of gaenari (forsythia) shoot blight caused by the fungus Septotinia sp., but also the first report on the genus Septotinia as a plant pathogen in Korea.
{"title":"First Report on the Shoot Blight Disease of Gaenari (Forsythia koreana) Caused by Septotinia sp. in Korea","authors":"Ji Hye Woo, Mikyeong Kim, Kun-Woo Lee, Sang-sup Han, Byeongjin Cha","doi":"10.5423/rpd.2023.29.3.234","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.234","url":null,"abstract":"In early spring, water-soaked lesions appeared on the petals and leaves of gaenari (<i>Forsythia koreana</i>), and the tissues were necrotic and dry. Cankers appeared on the infected branches around late spring and the above part of a branch withered and died. However, it was very rare that the base of the cankered-branch died. The identical fungi were isolated from the lesions on various tissues, and they grew with white colonies on potato dextrose agar medium. The fungus grew most actively at 23oC and produced many sclerotia of various sizes. In a pathogenicity assay in which mycelial and sclerotial suspensions were inoculated on each organ of forsythia, it was found that the pathogen infects the flower only, but not the leaves or branches. Symptoms on the flowers spread to the next leaves and branches over time and the infected branches were eventually withered. To identify the isolates, DNA sequences of four phylogenetic markers including ITS, LSU, Tub2, and CAL were analyzed and all isolates were identified as a species in the genus Septotinia. This is not only the first report of gaenari (forsythia) shoot blight caused by the fungus Septotinia sp., but also the first report on the genus <i>Septotinia</i> as a plant pathogen in Korea.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.295
Wan-Gyu Kim, Gyo-Bin Lee, Soon-Yeong Hong, Weon-Dae Cho
During a disease survey conducted in April 2022, we observed severe gray mold symptoms on inflorescences of mango trees (Mangifera indica) grown in a vinyl greenhouse in Jeju, Korea. The symptoms occurred on the flowers and peduncles, on which a lot of gray molds were formed. The incidence of gray mold on the inflorescences in the vinyl greenhouse ranged from 10% to 40%. Three fungal isolates were obtained from the lesions and identified as Botrytis cinerea based on their morphological characteristics and phylogenetic analysis. All isolates of B. cinerea were tested for their pathogenicity to inflorescences of mango trees through artificial inoculation. The pathogenicity of the isolates was confirmed on the flowers and peduncles. The symptoms induced by the isolates were similar to those on the inflorescences of mango trees observed in the disease survey. This is the first report of B. cinerea causing gray mold in mango in Korea.
{"title":"Occurrence of Gray Mold in Mango Caused by Botrytis cinerea in Korea","authors":"Wan-Gyu Kim, Gyo-Bin Lee, Soon-Yeong Hong, Weon-Dae Cho","doi":"10.5423/rpd.2023.29.3.295","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.295","url":null,"abstract":"During a disease survey conducted in April 2022, we observed severe gray mold symptoms on inflorescences of mango trees (<i>Mangifera indica</i>) grown in a vinyl greenhouse in Jeju, Korea. The symptoms occurred on the flowers and peduncles, on which a lot of gray molds were formed. The incidence of gray mold on the inflorescences in the vinyl greenhouse ranged from 10% to 40%. Three fungal isolates were obtained from the lesions and identified as <i>Botrytis cinerea</i> based on their morphological characteristics and phylogenetic analysis. All isolates of <i>B. cinerea</i> were tested for their pathogenicity to inflorescences of mango trees through artificial inoculation. The pathogenicity of the isolates was confirmed on the flowers and peduncles. The symptoms induced by the isolates were similar to those on the inflorescences of mango trees observed in the disease survey. This is the first report of <i>B. cinerea</i> causing gray mold in mango in Korea.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.243
Jung Wook Yang, Eun Young Kim, Jin Kyo Jung, In Jeong Kang, Yul Ho Kim, Boyng Joo Kim, Un Ho Yang, Sunggi Heu, Hyunjung Chung
To explore the distribution and the resistance reaction of rice blast pathogens that may occur in North Korea, rice blast pathogens in the North Korean border regions of Dandong and Yeon-gil in China and the North Korean border region of Cheorwon in South Korea were analyzed. In addition, comparative analysis was conducted with rice blast pathogen in Suwon and Jeonju, inland regions of South Korea. Resistance reactions above average were observed in monogenic rice lines (IRBLzt-T, IRBL9-W, IRBL20-IR24, and IRBLta-CP1) in Jeonju, Suwon, and Cheorwon from 2018 to 2020. In Dandong and Yeon-gil, the monogenic lines IRBLz5-CA, IRBL12-M, and IRBL19-A consistently showed resistance reactions for three years. Notably, IRBL19-A exhibited strong resistance. Race distribution analysis in South Korea indicated a shift from KI to KJ dominance from 2018 to 2020, while in the North Korean border regions of Dandong and Yeon-gil, the KI race was dominant in 2021 and 2022. The race distribution of rice blast pathogens in China's North Korean border regions differed significantly from that in South Korea.
{"title":"Analysis of the Distribution of Rice Blast Pathogens in High-Altitude North Korea Border Areas and Domestic Rice Cultivars","authors":"Jung Wook Yang, Eun Young Kim, Jin Kyo Jung, In Jeong Kang, Yul Ho Kim, Boyng Joo Kim, Un Ho Yang, Sunggi Heu, Hyunjung Chung","doi":"10.5423/rpd.2023.29.3.243","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.243","url":null,"abstract":"To explore the distribution and the resistance reaction of rice blast pathogens that may occur in North Korea, rice blast pathogens in the North Korean border regions of Dandong and Yeon-gil in China and the North Korean border region of Cheorwon in South Korea were analyzed. In addition, comparative analysis was conducted with rice blast pathogen in Suwon and Jeonju, inland regions of South Korea. Resistance reactions above average were observed in monogenic rice lines (IRBLzt-T, IRBL9-W, IRBL20-IR24, and IRBLta-CP1) in Jeonju, Suwon, and Cheorwon from 2018 to 2020. In Dandong and Yeon-gil, the monogenic lines IRBLz5-CA, IRBL12-M, and IRBL19-A consistently showed resistance reactions for three years. Notably, IRBL19-A exhibited strong resistance. Race distribution analysis in South Korea indicated a shift from KI to KJ dominance from 2018 to 2020, while in the North Korean border regions of Dandong and Yeon-gil, the KI race was dominant in 2021 and 2022. The race distribution of rice blast pathogens in China's North Korean border regions differed significantly from that in South Korea.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136277978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.310
Kyoung-Taek Park, Leonid N. Ten, Chang-Gi Back, Soo-Min Hong, Seung-Yeol Lee, Jeung-Sul Han, Hee-Young Jung
In May 2021, characteristic soft rot symptoms, including soft, watery, slimy, black rot, wilting, and leaf collapse, were observed on melon plants (Cucumis melo) in Gokseong, Jeollanam-do, Korea. A bacterial strain, designated KNUB-06-21, was isolated from infected plant samples, taxonomically classified, and phylogenetically analyzed using 16S rRNA and housekeeping gene sequencing. Strain KNUB-06-21 was also examined for compound utilization using the API ID 32 GN system and strain KNUB-06-21 was identified as Pectobacterium brasiliense. Subsequent melon stem inoculation studies using strain KNUB-06-21 showed soft rot symptoms similar to field plants. Re-isolated strains shared phenotypic and molecular characteristics with the original P. brasiliense KNUB-06-21 strain. To our knowledge, ours is the first report of P. brasiliense causing melon soft rot disease in Korea.
2021年5月，在全罗南道谷城的甜瓜(<i>Cucumis melo</i>)上发现了软腐病的特征性症状，包括软腐病、水腐病、粘稠病、黑腐病、萎蔫病、叶萎病等。从受感染的植物样本中分离出一株菌株，命名为KNUB-06-21，进行了分类，并利用16S rRNA和管家基因测序进行了系统发育分析。利用API ID 32 GN系统对菌株KNUB-06-21进行复合利用鉴定，鉴定菌株KNUB-06-21为巴西乳杆菌。随后使用菌株KNUB-06-21进行的甜瓜茎接种研究显示，软腐病症状与田间植株相似。重新分离的菌株与原菌株具有相同的表型和分子特征。brasiliense< / i>KNUB-06-21压力。据我们所知，我们的报告是第一次报告。brasiliense< / i>在韩国引起甜瓜软腐病。
{"title":"First Report of Melon Soft Rot Disease Caused by Pectobacterium brasiliense in Korea","authors":"Kyoung-Taek Park, Leonid N. Ten, Chang-Gi Back, Soo-Min Hong, Seung-Yeol Lee, Jeung-Sul Han, Hee-Young Jung","doi":"10.5423/rpd.2023.29.3.310","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.310","url":null,"abstract":"In May 2021, characteristic soft rot symptoms, including soft, watery, slimy, black rot, wilting, and leaf collapse, were observed on melon plants (<i>Cucumis melo</i>) in Gokseong, Jeollanam-do, Korea. A bacterial strain, designated KNUB-06-21, was isolated from infected plant samples, taxonomically classified, and phylogenetically analyzed using 16S rRNA and housekeeping gene sequencing. Strain KNUB-06-21 was also examined for compound utilization using the API ID 32 GN system and strain KNUB-06-21 was identified as Pectobacterium brasiliense. Subsequent melon stem inoculation studies using strain KNUB-06-21 showed soft rot symptoms similar to field plants. Re-isolated strains shared phenotypic and molecular characteristics with the original <i>P. brasiliense</i> KNUB-06-21 strain. To our knowledge, ours is the first report of <i>P. brasiliense</i> causing melon soft rot disease in Korea.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.268
Inkyu Lee, Young Sun Baek, Youn Su Lee
This study was conducted to confirm the utilization of Pleurospermum camtschaticum root extract as an organic agricultural material. Antioxidant activity of P. camtschaticum root extract, closely related to antibacterial activity, increased in a dose-dependent manner. In mycelial growth inhibitory activity, 100% P. camtschaticum root extract supressed over 70% for Colletotrichum coccodes and over 68% for Colletotrichum dematium. In the pepper fruit anthracnose development test, the size of the lesion decreased in a dose-dependent manner, which showed the same tendency as the previous results in inhibitory activity on mycelial growth. In the pepper seed germination and red pepper growth promotion test of P. camtschaticum root extract, oposite results was confirmed. The lower the concentration, the more the seed germination and growth promotion effects were shown. The phenol content of pepper leaves was also measured after pepper growth promotion test have been completed. The phenol content related to antibacterial activity increased in all treated groups compared to the untreated group. Therefore, the results of this study showed the possibility of development as an organic material.
{"title":"Determination of Antifungal Activity on Pepper Anthracnose and Plant Growth Promoting Activity of Pleurospermum camtschaticum Root Extract","authors":"Inkyu Lee, Young Sun Baek, Youn Su Lee","doi":"10.5423/rpd.2023.29.3.268","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.268","url":null,"abstract":"This study was conducted to confirm the utilization of <i>Pleurospermum camtschaticum</i> root extract as an organic agricultural material. Antioxidant activity of <i>P. camtschaticum</i> root extract, closely related to antibacterial activity, increased in a dose-dependent manner. In mycelial growth inhibitory activity, 100% <i>P. camtschaticum</i> root extract supressed over 70% for <i>Colletotrichum</i> coccodes and over 68% for <i>Colletotrichum dematium</i>. In the pepper fruit anthracnose development test, the size of the lesion decreased in a dose-dependent manner, which showed the same tendency as the previous results in inhibitory activity on mycelial growth. In the pepper seed germination and red pepper growth promotion test of <i>P. camtschaticum</i> root extract, oposite results was confirmed. The lower the concentration, the more the seed germination and growth promotion effects were shown. The phenol content of pepper leaves was also measured after pepper growth promotion test have been completed. The phenol content related to antibacterial activity increased in all treated groups compared to the untreated group. Therefore, the results of this study showed the possibility of development as an organic material.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.5423/rpd.2023.29.3.205
Myeong Hyeon Nam, Je Hyeok Yoo, Tugsang Yun, Hakhun Kim, Hong Gi Kim
Anthracnose caused by Colletotrichum spp. is a major disease of strawberry plants worldwide. The anthracnose of strawberries in Korea has occurred in most cultivated strawberry varieties since its first report in 1990. Although many studies and efforts have been made to control the disease, anthracnose continues to affect strawberry yields in Korea. In this review, we introduced various management methods to control anthracnose in strawberries, such as identifying pathogens and environmental factors involved in this disease, understanding the importance of latent infection, and developing diagnostic techniques and control methods. Through this review, we propose that researchers can elucidate a comprehensive and practical approach to control anthracnose by understanding this disease and developing strategies to prevent its spread, ultimately benefiting strawberry farmers.
{"title":"Anthracnose of Strawberry: Etiological and Ecological Characteristics, and Management","authors":"Myeong Hyeon Nam, Je Hyeok Yoo, Tugsang Yun, Hakhun Kim, Hong Gi Kim","doi":"10.5423/rpd.2023.29.3.205","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.3.205","url":null,"abstract":"Anthracnose caused by <i>Colletotrichum</i> spp. is a major disease of strawberry plants worldwide. The anthracnose of strawberries in Korea has occurred in most cultivated strawberry varieties since its first report in 1990. Although many studies and efforts have been made to control the disease, anthracnose continues to affect strawberry yields in Korea. In this review, we introduced various management methods to control anthracnose in strawberries, such as identifying pathogens and environmental factors involved in this disease, understanding the importance of latent infection, and developing diagnostic techniques and control methods. Through this review, we propose that researchers can elucidate a comprehensive and practical approach to control anthracnose by understanding this disease and developing strategies to prevent its spread, ultimately benefiting strawberry farmers.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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