Sumalatha A, Chandana Basalapura Rangegowda, Labdhi Dedhia, Lakshmana Reddy D C, Arivalagan M, Vijaya Bhaskar, Tejaswini Prakash
The area under marigold cultivation is increasing over the years and so is the demand for marigold seeds. To meet the increasing demand, hybrid varieties are preferred as they produce higher yields, for which the right parental selection is of major concern. Male sterility being the prerequisite for economical hybrid seed production of marigold, we have attempted to strategize the selection of male sterile seed parent and fertile pollen parent for yield and yield-related traits. The study was undertaken across multiple forms of male sterile lines morphologically varying in apetaloid and petaloid types, therefore use of BLUP and GCA was evaluated as a criterion to select the parents for the hybridization program. Results suggested apetaloid male sterile lines as better seed parents for days to bud initiation, while, petaloid male sterile lines can be selected for the improvement of shelf life and flower diameter. Results from BLUP and GCA were in agreement with each other for the traits studied. However, BLUP-based comparison of different lines is less tedious as it eliminates the laborious procedure of developing multiple hybrids and evaluating them to study the combining ability effects.
{"title":"Comparative analysis of BLUP and GCA for parental selection in marigold (Tagetes erecta L.) for hybrid development","authors":"Sumalatha A, Chandana Basalapura Rangegowda, Labdhi Dedhia, Lakshmana Reddy D C, Arivalagan M, Vijaya Bhaskar, Tejaswini Prakash","doi":"10.24154/jhs.v18i2.2106","DOIUrl":"https://doi.org/10.24154/jhs.v18i2.2106","url":null,"abstract":"The area under marigold cultivation is increasing over the years and so is the demand for marigold seeds. To meet the increasing demand, hybrid varieties are preferred as they produce higher yields, for which the right parental selection is of major concern. Male sterility being the prerequisite for economical hybrid seed production of marigold, we have attempted to strategize the selection of male sterile seed parent and fertile pollen parent for yield and yield-related traits. The study was undertaken across multiple forms of male sterile lines morphologically varying in apetaloid and petaloid types, therefore use of BLUP and GCA was evaluated as a criterion to select the parents for the hybridization program. Results suggested apetaloid male sterile lines as better seed parents for days to bud initiation, while, petaloid male sterile lines can be selected for the improvement of shelf life and flower diameter. Results from BLUP and GCA were in agreement with each other for the traits studied. However, BLUP-based comparison of different lines is less tedious as it eliminates the laborious procedure of developing multiple hybrids and evaluating them to study the combining ability effects.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140505536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Western Ghats are known for wild mangoes known for their distinctive flavours, tastes, and scents. The exploration of wild mangoes of coastal districts of Karnataka was undertaken. A total of 45 mango accessions were assessed for morphological characters (leaf and fruit) using numerical approach. The 14 traits including leaf blade length, leaf blade width, petiole length, fruit length, diameter, weight, and breadth, pulp, TSS, peel, and fruit thickness were analyzed. Fruit weight (g), stone weight (g), pulp (%), peel (%) and leaf blade length (cm) showed most diversity. The Moodbidri accession had most fruit weight (109.55 g), whereas, the Dakshina Kannada district’s Moodbidri accession had the lightest stone weight (12.72 g). It is the first documentation of the local mango germplasm variability in coastal Karnataka.
{"title":"Qualitative and quantitative assessment of wild genotypes of mango (Mangifera indica L.) in coastal districts of Karnataka, India","authors":"Ruchika Saliyan, Rama Bhat P, Ravishankar K V","doi":"10.24154/jhs.v18i2.1769","DOIUrl":"https://doi.org/10.24154/jhs.v18i2.1769","url":null,"abstract":"Western Ghats are known for wild mangoes known for their distinctive flavours, tastes, and scents. The exploration of wild mangoes of coastal districts of Karnataka was undertaken. A total of 45 mango accessions were assessed for morphological characters (leaf and fruit) using numerical approach. The 14 traits including leaf blade length, leaf blade width, petiole length, fruit length, diameter, weight, and breadth, pulp, TSS, peel, and fruit thickness were analyzed. Fruit weight (g), stone weight (g), pulp (%), peel (%) and leaf blade length (cm) showed most diversity. The Moodbidri accession had most fruit weight (109.55 g), whereas, the Dakshina Kannada district’s Moodbidri accession had the lightest stone weight (12.72 g). It is the first documentation of the local mango germplasm variability in coastal Karnataka.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140505428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A field study conducted with soil and foliar application of ZnSO4 and borax at different phenological stages of guava cv. Shewta in sandy loam soil, revealed that highest sustainable yield index (0.80) was recorded with two sprays of 0.4% ZnSO4 + 0.2% borax during fruit growth at one month interval, followed by 0.63 with three sprays of 0.4% ZnSO4 + 0.2% borax before flowering, fruit set and during fruit growth. Highest TSS (13.13°Brix), acidity (0.652%) and ascorbic acid (262.21 mg/100 g) was recorded in two sprays of 0.4% ZnSO4 + 0.2% borax at fruit growth in one month interval. Significant differences in Zn and B content in guava fruit pulp was recorded. It was noted that Guava fruit pulp had Zn content of 13.7 mg kg-1 in control tree fruits while 14.6 to 16.5 mg kg-1 in treated trees. Moreover, guava fruit pulp enriched with B (14.3 to 17.3 mg kg-1) in treated tree fruits as compared to 12.4 mg kg-1 in control trees. Micronutrient contents in leaf tissues showed significant difference in Zn and B concentration, whereas, Fe, Mn and Cu contents were statistically non-significant. The index suggested for attaining the sustainability and to economize the nutrient application, technology package consisting of two sprays of 0.4% ZnSO4 and 0.2% borax during fruit growth at one month interval should be adopted at growers’ field.
{"title":"Yield sustainability through micronutrient management in Guava","authors":"Tarun Adak, Kumar K, A. Ganeshamurthy, Pandey G","doi":"10.24154/jhs.v18i2.1860","DOIUrl":"https://doi.org/10.24154/jhs.v18i2.1860","url":null,"abstract":"A field study conducted with soil and foliar application of ZnSO4 and borax at different phenological stages of guava cv. Shewta in sandy loam soil, revealed that highest sustainable yield index (0.80) was recorded with two sprays of 0.4% ZnSO4 + 0.2% borax during fruit growth at one month interval, followed by 0.63 with three sprays of 0.4% ZnSO4 + 0.2% borax before flowering, fruit set and during fruit growth. Highest TSS (13.13°Brix), acidity (0.652%) and ascorbic acid (262.21 mg/100 g) was recorded in two sprays of 0.4% ZnSO4 + 0.2% borax at fruit growth in one month interval. Significant differences in Zn and B content in guava fruit pulp was recorded. It was noted that Guava fruit pulp had Zn content of 13.7 mg kg-1 in control tree fruits while 14.6 to 16.5 mg kg-1 in treated trees. Moreover, guava fruit pulp enriched with B (14.3 to 17.3 mg kg-1) in treated tree fruits as compared to 12.4 mg kg-1 in control trees. Micronutrient contents in leaf tissues showed significant difference in Zn and B concentration, whereas, Fe, Mn and Cu contents were statistically non-significant. The index suggested for attaining the sustainability and to economize the nutrient application, technology package consisting of two sprays of 0.4% ZnSO4 and 0.2% borax during fruit growth at one month interval should be adopted at growers’ field.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140506447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rishitha Lebaka, Benjamin Tuner, Shad Nelson, A. Anoruo
An experiment was conducted in the greenhouse to study growth, development, yield and water-use by Anahein pepper grown in a potting mixture supplemented with MiracleGro® (9:4:12) and milled alfalfa (3:1:2) as sources of nutrient. The study was comprised of 5 treatments, control (C), 3 gL-1 MiracleGro® (MG), 9 g alfalfa mill supplement (SA-1), 18 g (SA-2), and 27 g (SA-3), and replicated 6 times. Ten physiological and morphological parameters were used to evaluate growth, development and yield of the Anaheim pepper, and two parameters used to evaluate the water holding capacity of the potting mixture. The results indicate that the potting medium supplemented with alfalfa mill required significantly less water to support growth and development of the species. Also, growth, development and yield of Anaheim pepper was significantly higher in the organic supplements at SA-2 and SA-3.
{"title":"Studies on production of Anaheim pepper in greenhouse media supplemented with organic and inorganic nutrient sources, and water conservation","authors":"Rishitha Lebaka, Benjamin Tuner, Shad Nelson, A. Anoruo","doi":"10.24154/jhs.v18i2.2005","DOIUrl":"https://doi.org/10.24154/jhs.v18i2.2005","url":null,"abstract":"An experiment was conducted in the greenhouse to study growth, development, yield and water-use by Anahein pepper grown in a potting mixture supplemented with MiracleGro® (9:4:12) and milled alfalfa (3:1:2) as sources of nutrient. The study was comprised of 5 treatments, control (C), 3 gL-1 MiracleGro® (MG), 9 g alfalfa mill supplement (SA-1), 18 g (SA-2), and 27 g (SA-3), and replicated 6 times. Ten physiological and morphological parameters were used to evaluate growth, development and yield of the Anaheim pepper, and two parameters used to evaluate the water holding capacity of the potting mixture. The results indicate that the potting medium supplemented with alfalfa mill required significantly less water to support growth and development of the species. Also, growth, development and yield of Anaheim pepper was significantly higher in the organic supplements at SA-2 and SA-3.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140506647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mahananda Patil, T. Bharathi, T. Usharani, Rajiv Kumar, B. S. Kulkarni
A study was carried out to standardize the sterilization protocol for different explants (terminal stem scale,immature flower bud and tepal segment) and to select the suitable explant for the direct organogenesis of tuberose cv. Arka Vaibhav. The highest survival per cent (100) and uncontaminated cultures (0.00) of terminal stem scale explant was observed in pre-treatment with overnight soaking of terminal stem scale in the solution comprising carbendazim (0.1%), chlorothalonil (0.05%) and myristyl trimethyl ammonium bromide (cetrimide) (0.05%) and subsequently surface sterilization with 70% ethanol (1 min), 4% sodium hypochlorite (10 min) followed by 0.1% HgCl2 (15 min). The explant immature flower bud recorded the highest survival per cent (100) and maximum aseptic cultures in the treatment T1 comprised of 1.0 drop Tween-20 + 70% ethanol (30 sec) and 1% sodium hypochlorite (3 min). Pre-treatment of tepal segment explant in 0.1% carbendazim (30 min) solution followed by surface sterilization with combination of 1.0 drop Tween-20 + 70% ethanol (30 sec) followed by 1% sodium hypochlorite (3 min) registered 91.66% of survival with the minimum contamination (10%) in the treatment. Among the three explants used, the terminal stem scale was found suitable for direct organogenesis with early greenness (5.72 days) and highly responsive to shoot induction (100%) in MS medium supplemented with 4 mg/L BAP + 0.1mg/L IAA. Other two explants viz., immature flower bud and tepal segment failed to respond for direct organogenesis by shoot induction instead produced profuse callus.
{"title":"Standardization of sterilization protocol for explants and its suitability for direct organogenesis in tuberose cv. Arka Vaibhav","authors":"Mahananda Patil, T. Bharathi, T. Usharani, Rajiv Kumar, B. S. Kulkarni","doi":"10.24154/jhs.v18i1.2160","DOIUrl":"https://doi.org/10.24154/jhs.v18i1.2160","url":null,"abstract":"A study was carried out to standardize the sterilization protocol for different explants (terminal stem scale,immature flower bud and tepal segment) and to select the suitable explant for the direct organogenesis of tuberose cv. Arka Vaibhav. The highest survival per cent (100) and uncontaminated cultures (0.00) of terminal stem scale explant was observed in pre-treatment with overnight soaking of terminal stem scale in the solution comprising carbendazim (0.1%), chlorothalonil (0.05%) and myristyl trimethyl ammonium bromide (cetrimide) (0.05%) and subsequently surface sterilization with 70% ethanol (1 min), 4% sodium hypochlorite (10 min) followed by 0.1% HgCl2 (15 min). The explant immature flower bud recorded the highest survival per cent (100) and maximum aseptic cultures in the treatment T1 comprised of 1.0 drop Tween-20 + 70% ethanol (30 sec) and 1% sodium hypochlorite (3 min). Pre-treatment of tepal segment explant in 0.1% carbendazim (30 min) solution followed by surface sterilization with combination of 1.0 drop Tween-20 + 70% ethanol (30 sec) followed by 1% sodium hypochlorite (3 min) registered 91.66% of survival with the minimum contamination (10%) in the treatment. Among the three explants used, the terminal stem scale was found suitable for direct organogenesis with early greenness (5.72 days) and highly responsive to shoot induction (100%) in MS medium supplemented with 4 mg/L BAP + 0.1mg/L IAA. Other two explants viz., immature flower bud and tepal segment failed to respond for direct organogenesis by shoot induction instead produced profuse callus.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72380308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Megha Arun, C. Vasugi, M. Krishna Reddy, G. Narabenchi, R. Rajeshwari, K. Ravishankar, Linta Vincent
Papaya is one of the most important fruit crops of tropical and subtropical regions of the world including India. Though India stands first in production in the world, the productivity is low as compared to other countries due to high incidence of papaya ring spot virus (PRSV-P) attack. As all the cultivated varieties under genus Carica are susceptible to PRSV, investigations were carried out to evaluate fifteen intergeneric hybrid progenies of Arka Prabhath x V. cauliflora and eighty-five progenies of Arka Prabhath x V. cundinamarcencis for morphological, fruit and yield traits coupled with PRSV- P tolerance. Out of fifteen, seven progenies of ‘Arka Prabhath’ x V. cauliflora viz., IGHF1S4-1, IGHF1S4-12, IGHF1S4-13, IGHF1S4-14, IGHF1S4-15, IGHF1S4-17, IGHF1S4-18 and out of eighty-five, six progenies of ‘Arka Prabhath’ x V. cundinamarcensis viz., IGHF1S1-17, IGHF1S1-19, IGHF1S6-20, IGHF1S2-14, IGHF1S5-12 and IGHF1S5-14 recorded desirable traits such as days to first fruiting (240 to 250 days), bearing height (48 to 74 cm), plant height (175 to 200 cm), trunk circumference (37 to 48 cm), fruit weight (1133.67 to 2202.00 g), pulp thickness (2.45 to 4.05 cm), TSS (11.50 to 13.80 ºBrix), fruits/tree (40 to 58) and yield (45.00 to 78.20 kg/tree) coupled with PRSV tolerance with disease score1 (only a few tiny chlorotic spots on leaves). These progenies were selected and forwarded for next generation (F2). The hybridity was also confirmed using SSR marker (mCpCIR59).
木瓜是包括印度在内的世界热带和亚热带地区最重要的水果作物之一。虽然印度产量居世界首位，但由于番木瓜环斑病毒(PRSV-P)的高发病率，与其他国家相比，产量较低。由于Carica属栽培品种均对PRSV易感，对15个Arka prabhax V. cauliflora属间杂交后代和85个Arka prabhax V. cundinamarcensis属间杂交后代进行了形态、果实和产量性状与PRSV- P耐病性的比较研究。在15个子代中，7个‘Arka prabhax V. cauliflora即IGHF1S4-1, IGHF1S4-12, IGHF1S4-13, IGHF1S4-14, IGHF1S4-15, IGHF1S4-17, IGHF1S4-18;在85个子代中，6个‘Arka prabhax V. cundinamarcensis即IGHF1S1-17, IGHF1S1-19, IGHF1S6-20, IGHF1S2-14, IGHF1S5-12和IGHF1S5-14记录了理想的性状，如初结实天数(240至250天)，果实高度(48至74厘米)，株高(175至200厘米)，树干周长(37至48厘米)。果实重(1133.67 ~ 2202.00 g)、果肉厚度(2.45 ~ 4.05 cm)、TSS(11.50 ~ 13.80ºBrix)、果实/树数(40 ~ 58)和产量(45.00 ~ 78.20 kg/树)，以及抗PRSV能力和病分(叶片上只有几个小的褪绿斑点)。这些后代被选择并传递给下一代(F2)。利用SSR标记(mCpCIR59)对杂种性进行了验证。
{"title":"Evaluation of intergeneric F1 hybrid progenies of papaya (Arka Prabhath x Vasconcellea cauliflora and Arka Prabhath x Vasconcellea cundinamarcencis) for morphological, fruit and yield traits coupled with PRSV tolerance","authors":"Megha Arun, C. Vasugi, M. Krishna Reddy, G. Narabenchi, R. Rajeshwari, K. Ravishankar, Linta Vincent","doi":"10.24154/jhs.v18i1.2142","DOIUrl":"https://doi.org/10.24154/jhs.v18i1.2142","url":null,"abstract":"Papaya is one of the most important fruit crops of tropical and subtropical regions of the world including India. Though India stands first in production in the world, the productivity is low as compared to other countries due to high incidence of papaya ring spot virus (PRSV-P) attack. As all the cultivated varieties under genus Carica are susceptible to PRSV, investigations were carried out to evaluate fifteen intergeneric hybrid progenies of Arka Prabhath x V. cauliflora and eighty-five progenies of Arka Prabhath x V. cundinamarcencis for morphological, fruit and yield traits coupled with PRSV- P tolerance. Out of fifteen, seven progenies of ‘Arka Prabhath’ x V. cauliflora viz., IGHF1S4-1, IGHF1S4-12, IGHF1S4-13, IGHF1S4-14, IGHF1S4-15, IGHF1S4-17, IGHF1S4-18 and out of eighty-five, six progenies of ‘Arka Prabhath’ x V. cundinamarcensis viz., IGHF1S1-17, IGHF1S1-19, IGHF1S6-20, IGHF1S2-14, IGHF1S5-12 and IGHF1S5-14 recorded desirable traits such as days to first fruiting (240 to 250 days), bearing height (48 to 74 cm), plant height (175 to 200 cm), trunk circumference (37 to 48 cm), fruit weight (1133.67 to 2202.00 g), pulp thickness (2.45 to 4.05 cm), TSS (11.50 to 13.80 ºBrix), fruits/tree (40 to 58) and yield (45.00 to 78.20 kg/tree) coupled with PRSV tolerance with disease score1 (only a few tiny chlorotic spots on leaves). These progenies were selected and forwarded for next generation (F2). The hybridity was also confirmed using SSR marker (mCpCIR59).","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73487314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Survey was conducted at fortnightly intervals to assess the intensity of damage caused by the invasive whiteflies in coconut in the southern districts of Tamil Nadu viz., Thoothukudi, Tirunelveli, Tenkasi and Kanyakumari from December 2020 to August 2021. Among the four districts, Kanyakumari recorded the highest whitefly incidence (56.30%), whereas, Tenkasi showed the lowest infestation (48.83%). Two whitefly species viz., rugose spiralling whitefly, Aleurodicus rugioperculatus Martin and bondars nesting whitefly (BNW), Paraleyrodes bondari Peracchi were observed in all the surveyed districts. The rugose spiralling whitefly nymphs and adult populations were found to be highest in Kanyakumari (49.46 nymphs/leaflet; 36.99 adults/leaflet) and lowest in Tenkasi (32.76 nymphs/leaflet; 26.71 adults/leaflet). Similarly, the population of bondars nesting whitefly nymphs and adults were highest in Kanyakumari (35.31 nymphs/leaflet; 34.84 adults/leaflet), whereas, the lowest nymphal population was observed in Tenkasi (22.79 nymphs/leaflet) and adult population in Thoothukudi (24.19 adults/leaflet). In morphometric analysis, length and breadth of egg (0.24 ± 0.03 mm and 0.13± 0.02 mm), nymphal (0.83 ± 0.08 mm and 0.38 ± 0.04 mm), pupal (1.08 ± 0.09 mm and 0.70 ± 0.09 mm), adult (female: 2.59 ± 0.09mm, 1.71 ± 0.14 mm; male: 2.27 ± 0.21 mm, 1.30 ± 0.05 mm) was recorded for A. rugioperculatus and egg (0.15 ± 0.02 mm and 0.08 ± 0.01 mm), nymphal (0.46 ± 0.02 mm and 0.36 ± 0.02 mm), pupal (0.59 ± 0.16 mm and 0.41 ± 0.09 mm), adult (1.09 ± 0.08 mm and 0.73 ± 0.07 mm) for P. bondari.
{"title":"Seasonal incidence, population dynamics and morphometric traits of exotic coconut whiteflies in southern Tamil Nadu","authors":"S. Suriya, G. Preetha, N. Balakrishnan, J. Sheela","doi":"10.24154/jhs.v18i1.2167","DOIUrl":"https://doi.org/10.24154/jhs.v18i1.2167","url":null,"abstract":"Survey was conducted at fortnightly intervals to assess the intensity of damage caused by the invasive whiteflies in coconut in the southern districts of Tamil Nadu viz., Thoothukudi, Tirunelveli, Tenkasi and Kanyakumari from December 2020 to August 2021. Among the four districts, Kanyakumari recorded the highest whitefly incidence (56.30%), whereas, Tenkasi showed the lowest infestation (48.83%). Two whitefly species viz., rugose spiralling whitefly, Aleurodicus rugioperculatus Martin and bondars nesting whitefly (BNW), Paraleyrodes bondari Peracchi were observed in all the surveyed districts. The rugose spiralling whitefly nymphs and adult populations were found to be highest in Kanyakumari (49.46 nymphs/leaflet; 36.99 adults/leaflet) and lowest in Tenkasi (32.76 nymphs/leaflet; 26.71 adults/leaflet). Similarly, the population of bondars nesting whitefly nymphs and adults were highest in Kanyakumari (35.31 nymphs/leaflet; 34.84 adults/leaflet), whereas, the lowest nymphal population was observed in Tenkasi (22.79 nymphs/leaflet) and adult population in Thoothukudi (24.19 adults/leaflet). In morphometric analysis, length and breadth of egg (0.24 ± 0.03 mm and 0.13± 0.02 mm), nymphal (0.83 ± 0.08 mm and 0.38 ± 0.04 mm), pupal (1.08 ± 0.09 mm and 0.70 ± 0.09 mm), adult (female: 2.59 ± 0.09mm, 1.71 ± 0.14 mm; male: 2.27 ± 0.21 mm, 1.30 ± 0.05 mm) was recorded for A. rugioperculatus and egg (0.15 ± 0.02 mm and 0.08 ± 0.01 mm), nymphal (0.46 ± 0.02 mm and 0.36 ± 0.02 mm), pupal (0.59 ± 0.16 mm and 0.41 ± 0.09 mm), adult (1.09 ± 0.08 mm and 0.73 ± 0.07 mm) for P. bondari.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75724028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was conducted to establish an efficient in vitro plantlet regeneration protocol using the ex vitro leaves as explants for Haworthia retusa. Leaf tissues were cultured on liquid full-strength Murashige and Skoog (MS) medium supplemented with 2.0 mg/L indole 3-butyric acids (IBA) for callus induction, followed by sub-cultured to solid medium for callus proliferation. Callus was then transferred to a fresh medium supplemented with 6-benzyl amino adenine (BA) for shoot development. The result showed that the maximum rate of shoot regeneration (100%), number of shoots per explant (43), and shoot height (9.4 mm) were recorded on the solid MS medium supplemented with 1.0 mg/L BA and 30 g/L sucrose. IBA improved rooting, whereas, NAA (naphthaleneacetic acid) causes calli to form at the base of the shoots. The half-strength MS medium supplemented with 0.5 mg/L IBA provided the best rooting response for the shoot. This medium formulation resulted in the highest rooting rate (100%) and the highest mean root number (5 roots/explant). The result of the present study would be helpful for the mass propagation of commercially important H. retusa.
{"title":"Efficient in vitro plantlets regeneration from leaf explant of Haworthia retusa, an important ornamental succulent","authors":"Thi Trinh Huong, Tuan Trong Tran","doi":"10.24154/jhs.v18i1.2161","DOIUrl":"https://doi.org/10.24154/jhs.v18i1.2161","url":null,"abstract":"This study was conducted to establish an efficient in vitro plantlet regeneration protocol using the ex vitro leaves as explants for Haworthia retusa. Leaf tissues were cultured on liquid full-strength Murashige and Skoog (MS) medium supplemented with 2.0 mg/L indole 3-butyric acids (IBA) for callus induction, followed by sub-cultured to solid medium for callus proliferation. Callus was then transferred to a fresh medium supplemented with 6-benzyl amino adenine (BA) for shoot development. The result showed that the maximum rate of shoot regeneration (100%), number of shoots per explant (43), and shoot height (9.4 mm) were recorded on the solid MS medium supplemented with 1.0 mg/L BA and 30 g/L sucrose. IBA improved rooting, whereas, NAA (naphthaleneacetic acid) causes calli to form at the base of the shoots. The half-strength MS medium supplemented with 0.5 mg/L IBA provided the best rooting response for the shoot. This medium formulation resulted in the highest rooting rate (100%) and the highest mean root number (5 roots/explant). The result of the present study would be helpful for the mass propagation of commercially important H. retusa.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74445242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant extracts have been used as reducing and stabilising agents to synthesise various metal-based nanoparticles due to their cost-effective and eco-friendly nature. In the present work, a green and environment-friendly method is adopted for synthesising silver nanoparticles (Ag NPs) using a biowaste of dragon fruit (Hylocereus spp.) peel aqueous extract at 80ºC in an alkaline condition. The Ag NPs were characterised through various analytical and microscopic techniques. The UV-Vis spectra of Ag NPs showed a characteristic peak between 400 - 410 nm. Transmission and scanning electron microscopic studies confirmed spherical monodispersed particles with an average size of 7 nm. Energy-dispersive X-ray spectroscopy (EDX) confirmed the presence of silver and silver chloride among the principal elements. The X-ray powder diffraction (XRD) spectra showed the crystalline nature of synthesised silver and silver chloride nanoparticles. The synthesised nanoparticles showed potential antifungal activity against Colletotrichum truncatum spores in both in vitro conidial germination and spread plate assays. The efficacy of the synthesised NPs confirmed that these NPs could be used as potential antifungal agents against C. truncatum to control anthracnose in chilli.
{"title":"Dragon fruit peel extract mediated green synthesis of silver nanoparticles and their antifungal activity against Colletotrichum truncatum causing anthracnose in chilli","authors":"Shivakumar Gowda, S. Sriram","doi":"10.24154/jhs.v18i1.2165","DOIUrl":"https://doi.org/10.24154/jhs.v18i1.2165","url":null,"abstract":"Plant extracts have been used as reducing and stabilising agents to synthesise various metal-based nanoparticles due to their cost-effective and eco-friendly nature. In the present work, a green and environment-friendly method is adopted for synthesising silver nanoparticles (Ag NPs) using a biowaste of dragon fruit (Hylocereus spp.) peel aqueous extract at 80ºC in an alkaline condition. The Ag NPs were characterised through various analytical and microscopic techniques. The UV-Vis spectra of Ag NPs showed a characteristic peak between 400 - 410 nm. Transmission and scanning electron microscopic studies confirmed spherical monodispersed particles with an average size of 7 nm. Energy-dispersive X-ray spectroscopy (EDX) confirmed the presence of silver and silver chloride among the principal elements. The X-ray powder diffraction (XRD) spectra showed the crystalline nature of synthesised silver and silver chloride nanoparticles. The synthesised nanoparticles showed potential antifungal activity against Colletotrichum truncatum spores in both in vitro conidial germination and spread plate assays. The efficacy of the synthesised NPs confirmed that these NPs could be used as potential antifungal agents against C. truncatum to control anthracnose in chilli.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86124020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Callus induction was attempted from the four explants viz. root, cotyledon, epicotyl, and leaf segments excised from in vitro raised seedlings of C. jambhiri. Among various MS media supplementations with growth regulators and carbohydrates, the maximum (95.50%) and the earliest (8.30 days) callogenesis was obtained in epicotyl segments, when cultured on MS medium supplemented with NAA (10.0 mgl-1) + BAP (1.0 mgl-1) + sucrose (8%). The modified MS (macro and micro-nutrients reduced to half) fortified with BAP (5.0 mgl-1) + GA3 (3.0 mgl-1) recorded maximum shoot regeneration (43.10%) from callus, with an average of 5.30 shoots per callus after 35.50 days of culturing. However, prolonged exposure to GA3 resulted in thin elongated shoots and leaves. The age of the callus substantially influenced the plant regeneration frequency. The potency of the callus to regenerate decreased significantly with an increase in the age of the callus. Shoot regeneration was recorded maximum (43.43%) in 60 days old calli, followed by 90 days old (30.48%) calli, whereas it was minimum (10.46%) in 150 days old calli. The maximum (79.50%) shoot proliferation was recorded in MS medium supplemented with BAP (1.0 mgl-1) + Kin (0.5 mgl-1) with an average of 5.06 shoots per culture. The MS medium fortified with NAA (1.0 mgl-1) + IBA (1.0 mgl-1) induced maximum (77.33%) rooting, with an average of 3.19 roots per shoot after 13.4 days of culturing. Rooted plants were hardened and survived the best (83.6%) on the potting mixture consisting of cocopeat + vermiculite + perlite (2:1:1).
{"title":"Optimization of explants, media, plant growth regulators and carbohydrates on callus induction and plant regeneration in Citrus jambhiri Lush.","authors":"G. S. Sidhu, P. Malhotra, H. S. Rattanpal","doi":"10.24154/jhs.v18i1.2159","DOIUrl":"https://doi.org/10.24154/jhs.v18i1.2159","url":null,"abstract":"Callus induction was attempted from the four explants viz. root, cotyledon, epicotyl, and leaf segments excised from in vitro raised seedlings of C. jambhiri. Among various MS media supplementations with growth regulators and carbohydrates, the maximum (95.50%) and the earliest (8.30 days) callogenesis was obtained in epicotyl segments, when cultured on MS medium supplemented with NAA (10.0 mgl-1) + BAP (1.0 mgl-1) + sucrose (8%). The modified MS (macro and micro-nutrients reduced to half) fortified with BAP (5.0 mgl-1) + GA3 (3.0 mgl-1) recorded maximum shoot regeneration (43.10%) from callus, with an average of 5.30 shoots per callus after 35.50 days of culturing. However, prolonged exposure to GA3 resulted in thin elongated shoots and leaves. The age of the callus substantially influenced the plant regeneration frequency. The potency of the callus to regenerate decreased significantly with an increase in the age of the callus. Shoot regeneration was recorded maximum (43.43%) in 60 days old calli, followed by 90 days old (30.48%) calli, whereas it was minimum (10.46%) in 150 days old calli. The maximum (79.50%) shoot proliferation was recorded in MS medium supplemented with BAP (1.0 mgl-1) + Kin (0.5 mgl-1) with an average of 5.06 shoots per culture. The MS medium fortified with NAA (1.0 mgl-1) + IBA (1.0 mgl-1) induced maximum (77.33%) rooting, with an average of 3.19 roots per shoot after 13.4 days of culturing. Rooted plants were hardened and survived the best (83.6%) on the potting mixture consisting of cocopeat + vermiculite + perlite (2:1:1).","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89867357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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