A study was undertaken to assess the suitable drying methods for retention of quality parameters of drumstick (Moringa oleifera Lam.) leaf powder. The experiment was laid out in Factorial CRD involving three methods of drying (T1: Sun drying, T2: Shade drying and T3: Cabinet tray dryer) with three pre-treatments (B1: Unblanched, B2: Blanched with plain water and B3: Blanched followed by KMS dip) replicated three times. All the pre-treatments had significant effect on biochemical characteristics of drumstick leaves. Among the pre-treatments, unblanched leaves (B1) retained higher nutrient contents compared to other pre-treatments. The results ofthe investigation revealed that among the three different drying methods, shade dried sample was found to retain better nutritional properties. Significantly maximum values for moisture (11.18 %), ascorbic acid (156.27 mg/100g), vitamin-A (22.71 mg/100g), iron (16.54 mg/100g), oxalate (378.66 mg/100g), antioxidant activity (77.11%) and phenol (140.04 mg/100g) were recorded in shade dried sample. The interaction effect between pre-treatment and drying methods showed variation in results. However, the treatment combination T1B1 (Unblanched sun dried) was found to retain high protein (26.43 g/100g), magnesium (318.70 mg/100g) and potassium (1378.79 mg/100g) whereas T2B1 (unblanched shade dried) showed higher ascorbic acid (179.47 mg/100g), saponin (3.66 %), oxalate (541.47 mg/100g) and antioxidant (80.33 %) than rest of the treatment combinations.
{"title":"Effect of dehydration methods on quality parameters of drumstick (Moringa oleifera Lam.) leaf powder","authors":"Sahinur Ahmed, Langthasa S","doi":"10.24154/jhs.v17i1.1292","DOIUrl":"https://doi.org/10.24154/jhs.v17i1.1292","url":null,"abstract":"A study was undertaken to assess the suitable drying methods for retention of quality parameters of drumstick (Moringa oleifera Lam.) leaf powder. The experiment was laid out in Factorial CRD involving three methods of drying (T1: Sun drying, T2: Shade drying and T3: Cabinet tray dryer) with three pre-treatments (B1: Unblanched, B2: Blanched with plain water and B3: Blanched followed by KMS dip) replicated three times. All the pre-treatments had significant effect on biochemical characteristics of drumstick leaves. Among the pre-treatments, unblanched leaves (B1) retained higher nutrient contents compared to other pre-treatments. The results ofthe investigation revealed that among the three different drying methods, shade dried sample was found to retain better nutritional properties. Significantly maximum values for moisture (11.18 %), ascorbic acid (156.27 mg/100g), vitamin-A (22.71 mg/100g), iron (16.54 mg/100g), oxalate (378.66 mg/100g), antioxidant activity (77.11%) and phenol (140.04 mg/100g) were recorded in shade dried sample. The interaction effect between pre-treatment and drying methods showed variation in results. However, the treatment combination T1B1 (Unblanched sun dried) was found to retain high protein (26.43 g/100g), magnesium (318.70 mg/100g) and potassium (1378.79 mg/100g) whereas T2B1 (unblanched shade dried) showed higher ascorbic acid (179.47 mg/100g), saponin (3.66 %), oxalate (541.47 mg/100g) and antioxidant (80.33 %) than rest of the treatment combinations.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":"30 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83139858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meticulous analysis on intertwined interaction among the soil nutrients and microbial communities brings fruitful outcomes on horticulture. This study focuses on identifying well compatible bacterial genera in enhancing soil primary macro-nutrients for sustainable vegetable crops cultivation. The biochemical tests were executed for identification of bacterial genera. Eventually, mathematical models among available NPK nutrients (Nitrogen, Phosphorus and Potassium) and identified bacterial genera were derived to determine most suited bacterial genus for nutrients inhibition. This Study reveals the present nutrient’s status of soils at Anaimalai block covering 12,424 ha of Coimbatore district in Tamil Nadu. Seven utilitarian bacterial genera were identified which inhibit the plant nutrients. Among them, Azotobacter, Arthrobacter and Achromobacter actively inhibit available NPK in the soil. Present study of correlating soil nutrients with bacterial components enriches successful conservation of biosphere through adopting these innovative technologies in horticulture.
{"title":"Macronutrients and their associated bacterial genera in the soils of Anaimalai block in Tamil Nadu for sustainable vegetable crops cultivation","authors":"D. V, K. Sudalaimuthu","doi":"10.24154/jhs.v17i1.1198","DOIUrl":"https://doi.org/10.24154/jhs.v17i1.1198","url":null,"abstract":"Meticulous analysis on intertwined interaction among the soil nutrients and microbial communities brings fruitful outcomes on horticulture. This study focuses on identifying well compatible bacterial genera in enhancing soil primary macro-nutrients for sustainable vegetable crops cultivation. The biochemical tests were executed for identification of bacterial genera. Eventually, mathematical models among available NPK nutrients (Nitrogen, Phosphorus and Potassium) and identified bacterial genera were derived to determine most suited bacterial genus for nutrients inhibition. This Study reveals the present nutrient’s status of soils at Anaimalai block covering 12,424 ha of Coimbatore district in Tamil Nadu. Seven utilitarian bacterial genera were identified which inhibit the plant nutrients. Among them, Azotobacter, Arthrobacter and Achromobacter actively inhibit available NPK in the soil. Present study of correlating soil nutrients with bacterial components enriches successful conservation of biosphere through adopting these innovative technologies in horticulture.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":"48 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80830162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To determine the heat unit requirement and assess its subsequent effects on flowering and fruiting characteristics, a field experiment was conducted during 2018-19 with seven cultivars of litchi viz., Calcuttia, Elaichi, Bedana, Bombai, China, Shahi and Muzaffarpur in randomized block design. Bedana showed better result in terms of maximum fruit weight (17.88g), lowest seed content (10.84%), maximum fruit diameter (3.01 cm), maximum fruit volume (18.70 ml), highest TSS (15.870 Brix), total sugar (15.96%), reducing sugar (12.61%), and ascorbic acid (29.47 mg/100g) content.
{"title":"Heat unit requirement and performances of litchi under Sub-Himalayan terai region of West Bengal","authors":"N. Bhowmick, Saidiksha Subba","doi":"10.24154/jhs.v17i1.1202","DOIUrl":"https://doi.org/10.24154/jhs.v17i1.1202","url":null,"abstract":"To determine the heat unit requirement and assess its subsequent effects on flowering and fruiting characteristics, a field experiment was conducted during 2018-19 with seven cultivars of litchi viz., Calcuttia, Elaichi, Bedana, Bombai, China, Shahi and Muzaffarpur in randomized block design. Bedana showed better result in terms of maximum fruit weight (17.88g), lowest seed content (10.84%), maximum fruit diameter (3.01 cm), maximum fruit volume (18.70 ml), highest TSS (15.870 Brix), total sugar (15.96%), reducing sugar (12.61%), and ascorbic acid (29.47 mg/100g) content.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":"103 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84905461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The mango is considered as ‘king of fruits’ in India due to its delicious taste and nutritional status. Extension of fruit shelf life is a prime importance for availability of fresh fruit in market for longer duration and distance transportation. India is the largest producer and a prominent exporter of mango in the world.In this context, the study was conducted to evaluate the effect of preharvest spray of different chemicals and plant growth regulators (PGRs) on mango var. ‘Amrapali’ for shelf life and its quality. As ‘Amrapali’ has regular bearer with very good flavor and taste with a late maturing character, selected for shelf life studies. The fruits of mango weresprayed with chemicals viz. CaCl2 1%, CaCl2 2%, Ca(NO3)2 1%, Ca(NO3)2 2%, KNO3 1%, KNO3 2%, GA3 25 mg/l, GA3 50 mg/l, Ethrel 0.1 ml/l and Ethrel 0.2 ml/l prior to harvest. After harvesting, fruits were stored under ambient storage condition. Among all the treatments, GA3 25 mg/l treatment recorded significantly highest fruit length, fruit diameter, fruit volume and fruit weight at harvest and at fully ripe stage. Application of CaCl22% resulted in significantly minimum physiological loss in weight consistently from 2nd day to 16th day of storage besides significantly highest shelf life and quality. Hence, this intervention can contribute in preserving physical and chemical quality attributes for maximum acceptance by consumers.
{"title":"Effect of various pre-harvest treatments on shelf life and morphological characteristics of fruits of mango (Mangifera indica L.) var. ‘Amrapali’","authors":"P. Vishwakarma, M. Masu, Sumit Singh","doi":"10.24154/jhs.v17i1.909","DOIUrl":"https://doi.org/10.24154/jhs.v17i1.909","url":null,"abstract":"The mango is considered as ‘king of fruits’ in India due to its delicious taste and nutritional status. Extension of fruit shelf life is a prime importance for availability of fresh fruit in market for longer duration and distance transportation. India is the largest producer and a prominent exporter of mango in the world.In this context, the study was conducted to evaluate the effect of preharvest spray of different chemicals and plant growth regulators (PGRs) on mango var. ‘Amrapali’ for shelf life and its quality. As ‘Amrapali’ has regular bearer with very good flavor and taste with a late maturing character, selected for shelf life studies. The fruits of mango weresprayed with chemicals viz. CaCl2 1%, CaCl2 2%, Ca(NO3)2 1%, Ca(NO3)2 2%, KNO3 1%, KNO3 2%, GA3 25 mg/l, GA3 50 mg/l, Ethrel 0.1 ml/l and Ethrel 0.2 ml/l prior to harvest. After harvesting, fruits were stored under ambient storage condition. Among all the treatments, GA3 25 mg/l treatment recorded significantly highest fruit length, fruit diameter, fruit volume and fruit weight at harvest and at fully ripe stage. Application of CaCl22% resulted in significantly minimum physiological loss in weight consistently from 2nd day to 16th day of storage besides significantly highest shelf life and quality. Hence, this intervention can contribute in preserving physical and chemical quality attributes for maximum acceptance by consumers.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":"103 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78093645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Effect of rootstock on yield and quality of cucumber scion was studied at Department of Vegetable Science, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala during February-May, 2021. Parthenocarpic and non-parthenocarpic cucumber scions were grafted onto five different cultivated cucurbit species i.e. pumpkin, bottle gourd, oriental pickling melon, culinary melon and ash gourd. Significant variations were observed for all the traits under this study. The highest vine length (4.37 m) was observed in Heera scion grafted onto Lagenaria siceraria rootstock followed by Heera scion grafted onto Cucurbita moschata rootstock (4.13 m). The diameter of rootstock hypocotyl was higher in case of KPCH-1 grafted onto bottle gourd (1.48 mm) and Heera grafted onto bottle gourd (1.43 mm). KPCH-1 grafted on bottle gourd (29.33 days) and culinary melon (31 days) rootstocks showed early female flower initiation. The greater number of fruits was observed in graft combination of KPCH- 1 and bottle gourd (32) followed by parthenocarpic grafts with pumpkin (30.33) and ash gourd (30.33) rootstocks. A greater fruit weight was observed in graft combination of Heera and bottle gourd (7.51 kg) followed by Heera grafted onto pumpkin (7.38 kg). Results of this experiment suggest that these graft combinations can be employed in sustainable vegetable cultivation.
{"title":"Performance of parthenocarpic and non-parthenocarpic grafts of cucumber","authors":"Pooja P GOWDA, R. M., S. S","doi":"10.24154/jhs.v17i1.1188","DOIUrl":"https://doi.org/10.24154/jhs.v17i1.1188","url":null,"abstract":"Effect of rootstock on yield and quality of cucumber scion was studied at Department of Vegetable Science, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala during February-May, 2021. Parthenocarpic and non-parthenocarpic cucumber scions were grafted onto five different cultivated cucurbit species i.e. pumpkin, bottle gourd, oriental pickling melon, culinary melon and ash gourd. Significant variations were observed for all the traits under this study. The highest vine length (4.37 m) was observed in Heera scion grafted onto Lagenaria siceraria rootstock followed by Heera scion grafted onto Cucurbita moschata rootstock (4.13 m). The diameter of rootstock hypocotyl was higher in case of KPCH-1 grafted onto bottle gourd (1.48 mm) and Heera grafted onto bottle gourd (1.43 mm). KPCH-1 grafted on bottle gourd (29.33 days) and culinary melon (31 days) rootstocks showed early female flower initiation. The greater number of fruits was observed in graft combination of KPCH- 1 and bottle gourd (32) followed by parthenocarpic grafts with pumpkin (30.33) and ash gourd (30.33) rootstocks. A greater fruit weight was observed in graft combination of Heera and bottle gourd (7.51 kg) followed by Heera grafted onto pumpkin (7.38 kg). Results of this experiment suggest that these graft combinations can be employed in sustainable vegetable cultivation.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":"37 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86412365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Akshitha HJ, Prasath D, Umesha K, Mohammed Faisal P, Venkataravanappa V
Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to RAPD primers SSR primers were efficient in distinguishing the genotypes. A total of 86 and 23 polymorphic bands were observed with RAPD and SSR primers, respectively. Percentage polymorphism observed between RAPD and SSR primers was 97.40 % and 56.54 %. Grouping of genotypes by using combined data of RAPD and SSR primers indicated that irrespective of their place of collection or geographical origin, 30 genotypes were clustered into different groups which showed that, each individual genotype is having wider variability or it might be due to the genetic similarity existing among them.
{"title":"Molecular characterization of ginger genotypes using RAPD and SSR markers","authors":"Akshitha HJ, Prasath D, Umesha K, Mohammed Faisal P, Venkataravanappa V","doi":"10.24154/jhs.v17i1.1052","DOIUrl":"https://doi.org/10.24154/jhs.v17i1.1052","url":null,"abstract":"Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to RAPD primers SSR primers were efficient in distinguishing the genotypes. A total of 86 and 23 polymorphic bands were observed with RAPD and SSR primers, respectively. Percentage polymorphism observed between RAPD and SSR primers was 97.40 % and 56.54 %. Grouping of genotypes by using combined data of RAPD and SSR primers indicated that irrespective of their place of collection or geographical origin, 30 genotypes were clustered into different groups which showed that, each individual genotype is having wider variability or it might be due to the genetic similarity existing among them.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":"71 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74354799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amulya R N, Nagarajappa Adivappar, Shivakumar B S, Satish K M
Bael (Aegle marmelos (L) Correa) is an important underutilized fruit crop of India. A total of 25 bael trees were selected from 356 bael trees of Sakharayapattana in Chikkamagalur district, Karnataka, India based on the fruit morphological traits (fruit weight, pulp weight, skull thickness, seed weight per fruit, No. of seeds per fruit, No. of locules per fruit, No. of seeds per locule, pulp wt. : seed wt.). These 25 trees were evaluated for phenotypic and genotypic variations using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. RAPD and ISSR markers showed significant polymorphism among the trees. Jaccard’s genetic similarity value of RAPD and ISSR was found in the range of 0.00–0.95 and 0.06–0.56, respectively suggesting a moderate level of genetic diversity. The present study revealed that molecular markers can be successfully utilized for determining genetic diversity and relationship of bael trees for further varietal improvement.
{"title":"Studies on genetic variability and relationship of bael (Aegle marmelos (L) Correa) using morphological and molecular markers","authors":"Amulya R N, Nagarajappa Adivappar, Shivakumar B S, Satish K M","doi":"10.24154/jhs.v17i1.846","DOIUrl":"https://doi.org/10.24154/jhs.v17i1.846","url":null,"abstract":"Bael (Aegle marmelos (L) Correa) is an important underutilized fruit crop of India. A total of 25 bael trees were selected from 356 bael trees of Sakharayapattana in Chikkamagalur district, Karnataka, India based on the fruit morphological traits (fruit weight, pulp weight, skull thickness, seed weight per fruit, No. of seeds per fruit, No. of locules per fruit, No. of seeds per locule, pulp wt. : seed wt.). These 25 trees were evaluated for phenotypic and genotypic variations using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. RAPD and ISSR markers showed significant polymorphism among the trees. Jaccard’s genetic similarity value of RAPD and ISSR was found in the range of 0.00–0.95 and 0.06–0.56, respectively suggesting a moderate level of genetic diversity. The present study revealed that molecular markers can be successfully utilized for determining genetic diversity and relationship of bael trees for further varietal improvement.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":"15 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82308819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Two male sterile mutants IIHRRG-12MS (long fruited) and IIHRRG-28MS (medium long fruited) were identified from the ridge gourd germplasm IIHR-12 and IIHR-28 respectively at ICAR-IIHR, Bengaluru. These two male-sterile (ms) sources were characterized by the production of rudimentary male flowers in the racemes in contrast to the bright yellow flowers with fertile pollen and healthy anthers in male fertile, monoecious plants. Using these ms lines the inheritance of male sterility was worked out, which is cytoplasmic genic male sterility (CGMS) type, with single dominant gene either in homozygous or heterozygous condition restoring male fertility in the presence of sterile cytoplasm. In order to develop F1 hybrids using male sterility, several male sterile and maintainer lines were developed in different genetic back grounds such as green/dark green fruit colour and short/medium long/long fruit length.
{"title":"Characterization, inheritance of male sterility and development of male sterile and maintainer lines in ridge gourd (Luffa acutangula (Roxb.) L.)","authors":"V. B, Rajasekharan P.E","doi":"10.24154/jhs.v17i1.1007","DOIUrl":"https://doi.org/10.24154/jhs.v17i1.1007","url":null,"abstract":"Two male sterile mutants IIHRRG-12MS (long fruited) and IIHRRG-28MS (medium long fruited) were identified from the ridge gourd germplasm IIHR-12 and IIHR-28 respectively at ICAR-IIHR, Bengaluru. These two male-sterile (ms) sources were characterized by the production of rudimentary male flowers in the racemes in contrast to the bright yellow flowers with fertile pollen and healthy anthers in male fertile, monoecious plants. Using these ms lines the inheritance of male sterility was worked out, which is cytoplasmic genic male sterility (CGMS) type, with single dominant gene either in homozygous or heterozygous condition restoring male fertility in the presence of sterile cytoplasm. In order to develop F1 hybrids using male sterility, several male sterile and maintainer lines were developed in different genetic back grounds such as green/dark green fruit colour and short/medium long/long fruit length.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":"6 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78872982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jasmine occupies predominant position among the flower crops in India in terms of area, production and productivity. The demand for jasmine flowers is growing day by day owing to its wide range of uses and there is a pressing need for improving the crop by exploring strategies to evolve diverse genotypes. The present study focuses on the hybridization of Jasminum spp with the objective of introgression of desirable traits that would aid in creation of wider genetic variability. Pollination is the basis in any hybridization programme. The main aim of this research study was to determine the suitable pollination methods among self, open and cross pollination and to assess the effect of the pollination methods on the fruit set and fruit characteristics. The results of the study revealed that the overall response of J. auriculatum was found effective with maximum fruit set percentage. J. auriculatum cv Parimullai yielded the highest fruit set of 76.43% under open pollination and the least fruit set rate of 2.14% under self-pollination. Among the possible cross combination involving J. auriculatum and J. grandiflorum cultivars as seed parents with various pollen parents, J. flexile showed considerable results. Cross combination of J. auriculatum x J. flexile recorded maximum fruit set revealing best cross compatibility while crosses involving J. sambac resulted in no fruit set indicating the prevalence of fertilization barriers that hinder hybridization.
{"title":"Impact of pollination strategies on fruit set and fruit growth attributes in jasmine","authors":"Usha, Ganga M, Rajamani K, M. S, G. R","doi":"10.24154/jhs.v17i1.1376","DOIUrl":"https://doi.org/10.24154/jhs.v17i1.1376","url":null,"abstract":"Jasmine occupies predominant position among the flower crops in India in terms of area, production and productivity. The demand for jasmine flowers is growing day by day owing to its wide range of uses and there is a pressing need for improving the crop by exploring strategies to evolve diverse genotypes. The present study focuses on the hybridization of Jasminum spp with the objective of introgression of desirable traits that would aid in creation of wider genetic variability. Pollination is the basis in any hybridization programme. The main aim of this research study was to determine the suitable pollination methods among self, open and cross pollination and to assess the effect of the pollination methods on the fruit set and fruit characteristics. The results of the study revealed that the overall response of J. auriculatum was found effective with maximum fruit set percentage. J. auriculatum cv Parimullai yielded the highest fruit set of 76.43% under open pollination and the least fruit set rate of 2.14% under self-pollination. Among the possible cross combination involving J. auriculatum and J. grandiflorum cultivars as seed parents with various pollen parents, J. flexile showed considerable results. Cross combination of J. auriculatum x J. flexile recorded maximum fruit set revealing best cross compatibility while crosses involving J. sambac resulted in no fruit set indicating the prevalence of fertilization barriers that hinder hybridization.","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":"84 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85069698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A promising method for preparing metaphase spread for counting the number of chromosomes from the emerging shoot tissue is described in this report. In the present study, we adopted enzymatic digestion of shoot tips to analyse the chromosome number. The chromosomes in metaphase stage of cell division are highly condensed and easy to count in routine cytological technique. Even the morphological features like position of centromere can be seen in metaphase. In prophase it may not be clear as the chromosomes are getting ready for cell division. In enzymatic digestion even the prophase chromosomes are visible, which can be counted. Hence enzymatic digestion technique is more efficient in citrus as compared to acid digestion method as the citrus crop is a perennial crop with small-sized chromosomes. Furthermore, the sample collection in the field was easy and actively growing vegetative flush was available throughout the year. This technique was attempted in the tissue culture lab of ICAR- CCRI in various in vito and in vivo ploidy induction experiments in Citrus sinensis Osbeck (Sweet orange cv. mosambi), C. reticulata Blanco (Nagpur mandarin) and C. jambhiri Lush (Rough lemon), for confirmation of diploidy (2n=2x=18), triploidy (2n=3x=27), tetraploid (2n=4x=36), hexaploid (2n=6x=54).
本报告描述了一种有前途的方法，用于从新兴的芽组织中准备中期扩散以计数染色体的数量。在本研究中，我们采用茎尖酶切法分析染色体数目。细胞分裂中期的染色体高度浓缩，在常规细胞学技术中易于计数。中期甚至出现着丝粒位置等形态特征。在前期，当染色体准备进行细胞分裂时，这可能不太清楚。在酶解过程中，甚至可以看到可以计数的前期染色体。由于柑橘属多年生作物，染色体较小，因此酶解技术比酸解技术更有效。此外，在田间取样容易，并且全年都有积极生长的植物冲洗。本技术在ICAR- CCRI组织培养实验室进行了柑桔体内和体外倍体诱导实验。mosambi)， C. reticulata Blanco(那格浦尔柑橘)和C. jambhiri Lush(粗柠檬)，以确认二倍体(2n=2x=18)，三倍体(2n=3x=27)，四倍体(2n=4x=36)，六倍体(2n=6x=54)。
{"title":"Ploidy analysis among Citrus mutants using leaf meristematic tissue","authors":"Vijayakumari Narukulla, Y. Lahane, R. A","doi":"10.24154/jhs.v17i1.1186","DOIUrl":"https://doi.org/10.24154/jhs.v17i1.1186","url":null,"abstract":"A promising method for preparing metaphase spread for counting the number of chromosomes from the emerging shoot tissue is described in this report. In the present study, we adopted enzymatic digestion of shoot tips to analyse the chromosome number. The chromosomes in metaphase stage of cell division are highly condensed and easy to count in routine cytological technique. Even the morphological features like position of centromere can be seen in metaphase. In prophase it may not be clear as the chromosomes are getting ready for cell division. In enzymatic digestion even the prophase chromosomes are visible, which can be counted. Hence enzymatic digestion technique is more efficient in citrus as compared to acid digestion method as the citrus crop is a perennial crop with small-sized chromosomes. Furthermore, the sample collection in the field was easy and actively growing vegetative flush was available throughout the year. This technique was attempted in the tissue culture lab of ICAR- CCRI in various in vito and in vivo ploidy induction experiments in Citrus sinensis Osbeck (Sweet orange cv. mosambi), C. reticulata Blanco (Nagpur mandarin) and C. jambhiri Lush (Rough lemon), for confirmation of diploidy (2n=2x=18), triploidy (2n=3x=27), tetraploid (2n=4x=36), hexaploid (2n=6x=54).","PeriodicalId":36766,"journal":{"name":"Journal of Horticultural Sciences","volume":"10 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81646790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：