In present work, three press-hardened steel (PHS) sheets were designed for three typical manufacturing processes: cold-rolling (CR) process with about 1.5 km, thin slab continuous casting and rolling (TSCR) process with about 300 m, and Castrip process with about 50 m. Despite the similar constituents of mixed ferrite and pearlite, there were different microstructure characteristics and chemical distributions of these three PHS sheets. The press-hardened steel by Castrip process contained ultrafine pearlite lamella of about 198 nm, accompanying with high-density dislocations (∼1014 /m2). It was coarse pearlite lamella of 503 nm for press-hardened steel by TSCR process, while it was spheroidized pearlite with the average cementite particle size of 464 nm for press-hardened steel by CR process. The dislocation densities were ∼1013 and ∼1012 /m2 for press-hardened steels by TSCR and CR process, respectively. Subsequently, three press-hardened steel sheets were reheated to simulate the hot stamping process. From thermodynamics, press-hardened steel sheet by Castrip process could induce the earliest reversed austenite transformation due to more C and Mn. Kinetically, the high-density dislocations and ultrafine-lamella pearlite together created the fastest rate for reversed austenite transformation. Meanwhile, the mechanical properties of Castrip sheet could firstly reach 1500 MPa grade under the short-time heating condition (below 2 min). Furthermore, compared to the traditional one (900–950 °C for 3–5 min), the optimized hot stamping process of 930 °C × 2 min were performed on Castrip sheet by industrial plat die quenching process and real hot stamping part, which still reached 1500 MPa grade.