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Detection of Ciprofloxacin resistance genes in Escherichia coli isolated from dogs with urinary tract infections 犬尿路感染大肠杆菌中环丙沙星耐药基因的检测
Q4 Veterinary Pub Date : 2020-09-01 DOI: 10.22067/VETERINARY.V12I1.86316
M. Mustapha, P. Goel
This research was performed on uropathogenic Escherichia coli (E. coli) isolates and established the genes of resistance to ciprofloxacin between the isolates. A total of one hundred and three urine samples were tested for uropathogenic E. coli which were obtained from dogs with urinary tract infections (UTIs) using cultural isolation, antimicrobial susceptibility test, and polymerase chain reaction (PCR). The results revealed that genes associated with ciprofloxacin resistance are 24.3% positive for E. coli. The E. coli isolates were resistant to both ciprofloxacin and ampicillin (100%), highly susceptible to chloramphenicol (84.0%), and less susceptible to gentamycin (44.0%) and amikacin (40.0%). The PCR tests showed the presence of the ParC (in 25 samples; 100%), GyrA (in 25 samples; 100%), and GyrB (in 4 samples; 16.0%) genes. The findings of the present study showed an upsetting rate of ciprofloxacin and ampicillin resistance among the E. coli isolates from dogs with UTIs.
本研究对尿路致病性大肠杆菌(E.coli)分离株进行了研究,建立了分离株间对环丙沙星的抗性基因。使用培养分离、抗菌药物敏感性测试和聚合酶链式反应(PCR)对103份尿液样本进行了尿路致病性大肠杆菌检测,这些大肠杆菌是从患有尿路感染(UTIs)的狗身上获得的。结果表明,与环丙沙星耐药性相关的基因对大肠杆菌的阳性率为24.3%。大肠杆菌对环丙沙星和氨苄青霉素均具有耐药性(100%),对氯霉素高度敏感(84.0%),对庆大霉素和阿米卡星不太敏感(40.0%)。本研究的结果显示,在患有尿路感染的狗的大肠杆菌分离株中,环丙沙星和氨苄青霉素的耐药性降低。
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引用次数: 0
The Effects of administration of different parts of banana (Musa cavendish) fruit extracts and peel powder on the oxidative/antioxidative characteristics and some mineral concentrations in neonatal dairy calves 不同部位香蕉(Musa cavendish)果实提取物和果皮粉对新生奶牛氧化/抗氧化特性和某些矿物质浓度的影响
Q4 Veterinary Pub Date : 2020-09-01 DOI: 10.22067/VETERINARY.V12I1.85599
Nafiseh Keivani Rad, M. Mohri, H. Seifi, A. Haghparast
The present study examined the effects of overripe banana (Musa cavendish) pulp and green banana peel extract and powder on oxidants/antioxidants parameters and some minerals in Holstein dairy calves. Forty newborn calves were randomly divided into four groups of 10 (control, group one, group two and group three). The groups were homogenous for the parity of the dams and the time of birth.Within 12-48 hours of birth, calves were assigned to their treatment groups. In the control group, animals received no banana meal. In group one, calves were supplemented with 2 g (dry matter)/kg body weight/day of overripe banana pulp extract for five days. The calves in group two were supplemented with 1 g (dry matter) of overripe banana pulp extract /kg body weight/day and 1 g (dry matter) of green banana peel extract/kg body weight/day for five days. The animals in group three were supplemented with 2 g/kg body weight/day of green banana peel powder for five days. Blood samples were taken on day 0 (at birth) and on days 7, 15 and 30 through the jugular vein. Age (the time of sampling) had a significant effect on the values of phosphorous, potassium, iron, copper, FRAP and activity of GPx enzyme (p < 0.05). Significant group and sampling time interaction was seen for the FRAP concentrations (p < 0.05). In conclusion, banana supplementation in the Holstein dairy calf’s diet at the concentration and duration that was reported in the present study had beneficial effect on the values of FRAP.
本研究考察了过熟香蕉(Musa cavendish)果肉、青香蕉皮提取物和粉末对荷斯坦小牛氧化剂/抗氧化剂参数和某些矿物质的影响。将40头新生小牛随机分为4组,每组10头(对照组、第一组、第二组和第三组)。这两组在母鼠的性别和出生时间上是同质的。在出生后的12-48小时内,小牛被分配到各自的治疗组。在对照组中,动物不接受香蕉餐。在第一组中,小牛每天补充2克(干物质)/公斤体重的过熟香蕉浆提取物,为期五天。第二组中的小牛用1g(干物质)过熟香蕉浆提取物/kg体重/天和1g(干燥物质)青香蕉皮提取物/kg体重-天补充5天。第三组动物每天补充2g/kg体重的绿色香蕉皮粉,持续5天。在第0天(出生时)和第7、15和30天通过颈静脉采集血样。年龄(采样时间)对磷、钾、铁、铜、FRAP值和GPx酶活性有显著影响(p<0.05),以本研究中报道的浓度和持续时间在荷斯坦奶牛日粮中补充香蕉对FRAP值有有益影响。
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引用次数: 1
The effects of bedding materials on learning and memory performance and texture preference in rats 床上用品对大鼠学习记忆能力和质地偏好的影响
Q4 Veterinary Pub Date : 2020-09-01 DOI: 10.22067/VETERINARY.V12I1.86943
M. Abbasnejad, R. Kooshki, S. Esmaeili-Mahani, Abbas Tajabadi, R. Naderi
The present study was designed to investigate the effect of different available bedding materials on learning and memory performance, bedding texture preference as well as intra-cage ammonia concentration in rats. The animals were housed on different bedding types for two weeks. Bedding materials were produced in the same sizes from poplar, walnut, pistachio, apricot, almond woods and alfalfa steam and live. Spatial and passive avoidance learning and memory were assessed by Morris water maze (MWM) and shuttle box tasks. A modifying six-arm radial maze was used to assess bedding texture preference by rats. For each bedding groups, average ammonia level (ppm) over a week was calculated. The data indicated that the rats that had walnut and almond chips show better learning and memory performance in both MWM and shuttle box tests than other groups. The weakest learning and memory performances were observed in rats exposed to alfalfa bedding. In texture preference test, the rats spent more time in walnut and almond arms, and less time in alfalfa. Besides, the total water and food intake as well as the number of visit to alfalfa arm were decreased as compared to other arms. Moreover, in alfalfa bedding cage, average intra-cage ammonia level was utmost. Overall, current bedding materials may contain diverse biochemically effective compounds or individual micro edges which alter learning and memory performances of rats.
本研究旨在探讨不同床上用品对大鼠学习记忆性能、床上用品质地偏好及笼内氨浓度的影响。这些动物被安置在不同类型的床上两周。用杨木、核桃木、开心果木、杏木、杏木和紫花苜蓿蒸煮和活木制作相同尺寸的床上材料。采用Morris水迷宫(MWM)和穿梭箱任务评估空间和被动回避学习记忆。采用改进的六臂径向迷宫评价大鼠对床上用品质地的偏好。对于每个床上用品组,计算一周内的平均氨水平(ppm)。数据表明,吃核桃和杏仁片的大鼠在MWM和穿梭箱测试中都比其他组表现出更好的学习和记忆表现。紫花苜蓿垫料处理的大鼠学习记忆能力最差。在纹理偏好测试中,大鼠在核桃和杏仁手臂上花的时间更多,在苜蓿手臂上花的时间更少。此外,紫花苜蓿组的总饮水量和总摄取量以及访视次数均低于其他组。在紫花苜蓿床上笼中,平均笼内氨氮水平最高。总的来说,目前的床上材料可能含有多种生物化学有效化合物或单个微边缘,这些物质会改变大鼠的学习和记忆性能。
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引用次数: 0
Biological effects of agricultural bio-materials on some blood and tissue factors in Balb/c mice 农业生物材料对Balb/c小鼠某些血液和组织因子的生物学效应
Q4 Veterinary Pub Date : 2020-09-01 DOI: 10.22067/VETERINARY.V12I1.83273
M. Rostaminia, D. Habibi, S. Shahbazi, B. Sani, A. Pazoki
Pseudomonas infections are an important cause of morbidity and mortality and saprophytic fungi are now increasingly being recognized as serious pathogens in immunocompromised patients.To investigate the effect of using bio-materials on mammalian tissues,two experiments were designed;the first one was feeding of Balb/c mice with irrigated lettuce with bio-fungicide (mutant and wild)and bio-fertilizers prepared with Pseudomonas (p) fluorescens, p. putida, p. aeruginosa, and the second was the usage of drinking water containing (Trichoderma (T) spores (mutant and wild) or P.fluorescens, P.putida, P.aeruginosa suspensions). Then, blood factors and inflammation of tissues (liver, kidney, spleen and large intestine) in all mice were analyzed after two months. Blood samples were taken from the mice to examine some of the hematological (RBC, MCV, MCH, MCHC) (data not shown) and biochemical (AST, ALT, ALP) factors, and also observed under a microscope. The study of tumor marker carcinoembryonic antigen (CEA) in all treatments showed that the strains in these bio-fertilizers did not stimulate carcinogenic indices.The results from the other blood factors were normal for all strains (data not shown).Only P.putida showed no adverse effect on the increase in alkaline phosphatase (ALP).The results also showed that the effect of bio-fungicide on mammalian tissues (spleen and large intestine) was normal. But a small number of mild liver necrosis was seen in the treatment groups with wild Trichoderma, and moderate necrosis in the the liver tissue after treatment with mutant Trichoderma isolates.More investigation should be made to determine the impact of these biotic factors on the mammalian tissues before commercialization.
假单胞菌感染是发病率和死亡率的重要原因,腐生真菌现在越来越被认为是免疫功能低下患者的严重病原体。为了研究使用生物材料对哺乳动物组织的影响,设计了两个实验;第一种是用含有生物杀菌剂(突变体和野生)的灌溉莴苣和用荧光假单胞菌、恶臭假单胞菌和铜绿假单胞菌制备的生物肥料喂养Balb/c小鼠,第二种是使用含有(木霉(T)孢子(突变体和野化)或荧光假单胞杆菌、恶臭假单孢菌、铜绿假单胞杆菌悬浮液)的饮用水。然后,在两个月后分析所有小鼠的血液因素和组织(肝、肾、脾和大肠)的炎症。从小鼠身上采集血样以检查一些血液学(RBC、MCV、MCH、MCHC)(数据未显示)和生化(AST、ALT、ALP)因子,并在显微镜下观察。对所有处理中的肿瘤标志物癌胚抗原(CEA)的研究表明,这些生物肥料中的菌株不会刺激致癌指数。其他血液因素的结果对所有菌株都是正常的(数据未显示)。只有P.putida对碱性磷酸酶(ALP)的增加没有不良影响。结果还表明,生物杀菌剂对哺乳动物组织(脾脏和大肠)的影响是正常的。但野生木霉治疗组出现少量轻度肝坏死,突变木霉分离株治疗后肝组织出现中度坏死。在商业化之前,应该进行更多的研究来确定这些生物因素对哺乳动物组织的影响。
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引用次数: 1
The association between erythropoietin, vitamin D3 and parathormone levels and kidney lesions in buffaloes (Bubalus bubalis). 促红细胞生成素、维生素D3和甲状旁激素水平与水牛肾脏病变的关系。
Q4 Veterinary Pub Date : 2020-09-01 DOI: 10.22067/VETERINARY.V12I1.85313
Zana Shahabi, M. Jalali, B. Mohamadian, M. H. Hajikolaei, M. Jalali
Many diseases, including renal disorders in ruminants, appear in subclinical form and lead to reduced production without causing clinical signs. In this research, blood and tissue samples of kidney were taken from 102 buffaloes from Ahvaz industrial abattoir and sent to the laboratory for biochemical and histopathological analysis. After preparing the pathological sections from kidney specimens, conventional and specific staining and microscopic examination, 41 samples had no kidney lesion was considered as control and 61 specimens as the lesion group. The samples with renal lesions were divided into 4 subgroups including acute tubulointerstitial nephritis, chronic tubulointerstitial nephritis, Urinary tubular inflammation, and Congestion. The samples were also divided and compared to males and females. In blood samples, serum biochemical tests including parathyroid hormone, erythropoietin, and vitamin D3 were measured by using ELISA-specific commercial kits. After statistical analysis, the results of vitamin D3 in the studied groups did not show any significant difference. However, erythropoietin and parathormone values in buffaloes with renal lesions were significantly lower and higher than those in control animals respectively. The results of this study showed that most of the kidney lesions in the studied buffaloes including the mentioned above have led to a decrease in the production of erythropoietin hormone following the occurrence of these lesions and its functional effects on the kidneys. Naturally, the effects of this hormone deficiency are expected to be reflected in the hemogram and eventually, anemia resulting from it may affect overall body health.
许多疾病,包括反刍动物的肾脏疾病,以亚临床形式出现,导致产量减少,而不会引起临床症状。本研究从阿瓦士工业屠宰场的102头水牛身上采集血液和肾脏组织样本,送至实验室进行生化和组织病理学分析。肾标本病理切片制备、常规及特异性染色及镜检后,41例未见肾脏病变为对照组,61例为病变组。将肾脏病变标本分为急性肾小管间质性肾炎、慢性肾小管间质性肾炎、尿小管炎症和充血4个亚组。这些样本也被分为男性和女性。在血液样本中,血清生化测试包括甲状旁腺激素、促红细胞生成素和维生素D3使用elisa特异性商业试剂盒。经统计分析,各组维生素D3含量无显著差异。然而,肾脏病变水牛的促红细胞生成素和甲状旁激素值分别显著低于和高于对照动物。本研究结果表明,包括上述在内的大多数所研究的水牛肾脏病变,在这些病变发生后,红细胞生成素激素的产生及其对肾脏的功能影响都有所减少。当然,这种激素缺乏的影响预计会在血象中反映出来,最终,由此导致的贫血可能会影响全身健康。
{"title":"The association between erythropoietin, vitamin D3 and parathormone levels and kidney lesions in buffaloes (Bubalus bubalis).","authors":"Zana Shahabi, M. Jalali, B. Mohamadian, M. H. Hajikolaei, M. Jalali","doi":"10.22067/VETERINARY.V12I1.85313","DOIUrl":"https://doi.org/10.22067/VETERINARY.V12I1.85313","url":null,"abstract":"Many diseases, including renal disorders in ruminants, appear in subclinical form and lead to reduced production without causing clinical signs. In this research, blood and tissue samples of kidney were taken from 102 buffaloes from Ahvaz industrial abattoir and sent to the laboratory for biochemical and histopathological analysis. After preparing the pathological sections from kidney specimens, conventional and specific staining and microscopic examination, 41 samples had no kidney lesion was considered as control and 61 specimens as the lesion group. The samples with renal lesions were divided into 4 subgroups including acute tubulointerstitial nephritis, chronic tubulointerstitial nephritis, Urinary tubular inflammation, and Congestion. The samples were also divided and compared to males and females. In blood samples, serum biochemical tests including parathyroid hormone, erythropoietin, and vitamin D3 were measured by using ELISA-specific commercial kits. After statistical analysis, the results of vitamin D3 in the studied groups did not show any significant difference. However, erythropoietin and parathormone values in buffaloes with renal lesions were significantly lower and higher than those in control animals respectively. The results of this study showed that most of the kidney lesions in the studied buffaloes including the mentioned above have led to a decrease in the production of erythropoietin hormone following the occurrence of these lesions and its functional effects on the kidneys. Naturally, the effects of this hormone deficiency are expected to be reflected in the hemogram and eventually, anemia resulting from it may affect overall body health.","PeriodicalId":36826,"journal":{"name":"Iranian Journal of Veterinary Science and Technology","volume":"12 1","pages":"29-36"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44018513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-quorum sensing and antibacterial activities of Satureja sahendica hydroalcoholic extract against avian isolate of Salmonella Typhimurium 红花水醇提取物对鼠伤寒沙门菌禽分离物的抗群体感应及抑菌活性
Q4 Veterinary Pub Date : 2020-09-01 DOI: 10.22067/VETERINARY.V12I1.85433
Razieh Sharchi, J. Shayegh, S. Hosseinzadeh
Quorum sensing (QS) is a cell density dependent mechanism used by many pathogenic bacteria for regulating virulence gene expression. Inhibition or interruption of QS by herbal remedies has been suggested as a new strategy for fighting against antibiotic resistant bacteria. The aim of this study was to evaluate the antibacterial activity of Satureja sahendica hydroalcoholic extract (SSHE) against Salmonella Typhimurium (S. Typhimurium) isolates, and to assess the impact of sub-inhibitory concentrations of this extract on the QS-associated gene (sdiA) expression. Using the Soxhlet method, hydroalcoholic extract of S. sahendica leaves was prepared and antimicrobial effects of the SSHE were determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methodS. The reverse transcription quantitative PCR (RT-qPCR) assay was used to analyze the expression of sdiA in 20 S. Typhimurium isolates from poultry flocks in response to the treatment of sub-inhibitory concentrations of SSHE at 60-min time point. The MIC values of SSHE against S. Typhimurium isolates were ranged from 0.29-4.68 mg/ml-1 and MBC values were ranging from 75-150 mg/ml-1. The results also indicated that the expression of sdiA gene was reduced in S. Typhimurium isolates triggered by the treatment of SSHE comparatively with those from the control cultureS. Findings suggest that SSHE possess the antibacterial and anti-QS activity and can be used to control the expression of virulence genes in pathogenic bacteria, such as S. Typhimurium.
群体感应(QS)是许多病原菌用来调节毒力基因表达的一种细胞密度依赖性机制。草药抑制或阻断QS已被认为是对抗抗生素耐药性细菌的一种新策略。本研究的目的是评估沙参水醇提取物(SSHE)对鼠伤寒沙门氏菌(S.Typhimurium)分离株的抗菌活性,并评估该提取物的亚抑制浓度对QS相关基因(sdiA)表达的影响。采用Soxhlet法,制备了沙参叶的水醇提取物,并用最小抑菌浓度(MIC)和最小杀菌浓度(MBC)法测定了SSHE的抗菌效果。使用逆转录定量PCR(RT-qPCR)分析来自家禽群的20个鼠伤寒沙门氏菌分离株中sdiA的表达,以响应在60分钟时间点亚抑制浓度的SSHE的处理。SSHE对鼠伤寒沙门氏菌分离株的MIC值范围为0.29-4.68 mg/ml-1,MBC值范围为75-150 mg/ml-1。结果还表明,与对照培养物相比,由SSHE处理引发的鼠伤寒沙门氏菌分离物中sdiA基因的表达降低。研究结果表明,SSHE具有抗菌和抗QS活性,可用于控制致病菌(如鼠伤寒杆菌)毒力基因的表达。
{"title":"Anti-quorum sensing and antibacterial activities of Satureja sahendica hydroalcoholic extract against avian isolate of Salmonella Typhimurium","authors":"Razieh Sharchi, J. Shayegh, S. Hosseinzadeh","doi":"10.22067/VETERINARY.V12I1.85433","DOIUrl":"https://doi.org/10.22067/VETERINARY.V12I1.85433","url":null,"abstract":"Quorum sensing (QS) is a cell density dependent mechanism used by many pathogenic bacteria for regulating virulence gene expression. Inhibition or interruption of QS by herbal remedies has been suggested as a new strategy for fighting against antibiotic resistant bacteria. The aim of this study was to evaluate the antibacterial activity of Satureja sahendica hydroalcoholic extract (SSHE) against Salmonella Typhimurium (S. Typhimurium) isolates, and to assess the impact of sub-inhibitory concentrations of this extract on the QS-associated gene (sdiA) expression. Using the Soxhlet method, hydroalcoholic extract of S. sahendica leaves was prepared and antimicrobial effects of the SSHE were determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methodS. The reverse transcription quantitative PCR (RT-qPCR) assay was used to analyze the expression of sdiA in 20 S. Typhimurium isolates from poultry flocks in response to the treatment of sub-inhibitory concentrations of SSHE at 60-min time point. The MIC values of SSHE against S. Typhimurium isolates were ranged from 0.29-4.68 mg/ml-1 and MBC values were ranging from 75-150 mg/ml-1. The results also indicated that the expression of sdiA gene was reduced in S. Typhimurium isolates triggered by the treatment of SSHE comparatively with those from the control cultureS. Findings suggest that SSHE possess the antibacterial and anti-QS activity and can be used to control the expression of virulence genes in pathogenic bacteria, such as S. Typhimurium.","PeriodicalId":36826,"journal":{"name":"Iranian Journal of Veterinary Science and Technology","volume":"12 1","pages":"71-79"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46729320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Effect of four local anesthetics (tetracaine, bupivacaine, lidocaine and proparacaine) on intraocular pressure in rabbits- Comparison of an applanation and a rebound tonometer 四种局部麻醉剂(丁卡因、布比卡因、利多卡因和丙卡因)对兔眼压的影响——压平眼压计和回弹眼压计的比较
Q4 Veterinary Pub Date : 2020-04-12 DOI: 10.22067/VETERINARY.V12I1.82719
Arman Emrarian, A. A. Sarchahi
The type of device used, the type of local anesthetic agents, and the animal species may affect the intraocular pressure(IOP). Therefore, in order to determine these issues, the effects of four local anesthetics were investigated in 10 adult rabbits by ICare TA01i and Tono-Pen Vet tonometers. In the right eye of half of the rabbits and in the left eye of the other half of the rabbits, one drop of tetracaine was instilled. The IOP in each rabbit was measured using two tonometers, ICare and Tono-Pen Vet, before and each 5 minutes until 40 minutes later. The effects of other drugs were also studied at least with one-week interval. Based on the results of ICare tonometer, tetracaine significantly reduced the IOP immediately and 25 minutes after instillation. IOP changes after instillation of bupivacaine, lidocaine and proparacaine were not significant at any time compared to baseline values (p > 0.05). Based on the results of Tono-Pen Vet tonometer, all drugs reduced the IOP immediately after use; however, the effects of bupivacaine and lidocaine on IOP were much lower than that of tetracaine and proparacaine. The average duration of corneal anesthesia were 20, 15.5, 7.5 and 21 minutes for tetracaine, bupivacaine, lidocaine, and proparacaine, respectively. It is concluded that IOP reduction by local anesthetics when Tono-Pen Vet is used is much greater than the ICare tonometer measurements. Also, the reduction of IOP with each of the devices when tetracaine or proparacaine is used is greater than when bupivacaine or lidocaine is used.
使用的器械类型、局部麻醉剂类型和动物种类都可能影响眼内压(IOP)。因此,为了确定这些问题,我们采用ICare TA01i和Tono-Pen Vet眼压计对10只成年家兔进行了4种局麻药的影响研究。在一半兔的右眼和另一半兔的左眼各滴入一滴丁卡因。使用ICare和Tono-Pen Vet两种眼压计测量每只兔的眼压,每5分钟测量一次,直到40分钟后。其他药物的效果也至少间隔一周进行研究。根据ICare血压计的结果,丁卡因在注射后立即和25分钟显著降低IOP。布比卡因、利多卡因和丙帕卡因在任何时间内的IOP变化与基线值相比均无显著性差异(p < 0.05)。根据Tono-Pen Vet眼压计的结果,所有药物在使用后立即降低IOP;但布比卡因和利多卡因对IOP的影响远低于丁卡因和丙帕卡因。丁卡因、布比卡因、利多卡因和丙帕卡因的平均角膜麻醉时间分别为20、15.5、7.5和21分钟。我们得出结论,当使用Tono-Pen Vet时,局麻药的IOP降低幅度远大于ICare眼压计测量值。此外,当使用丁卡因或丙帕卡因时,每一种装置对IOP的降低都大于使用布比卡因或利多卡因时。
{"title":"Effect of four local anesthetics (tetracaine, bupivacaine, lidocaine and proparacaine) on intraocular pressure in rabbits- Comparison of an applanation and a rebound tonometer","authors":"Arman Emrarian, A. A. Sarchahi","doi":"10.22067/VETERINARY.V12I1.82719","DOIUrl":"https://doi.org/10.22067/VETERINARY.V12I1.82719","url":null,"abstract":"The type of device used, the type of local anesthetic agents, and the animal species may affect the intraocular pressure(IOP). Therefore, in order to determine these issues, the effects of four local anesthetics were investigated in 10 adult rabbits by ICare TA01i and Tono-Pen Vet tonometers. In the right eye of half of the rabbits and in the left eye of the other half of the rabbits, one drop of tetracaine was instilled. The IOP in each rabbit was measured using two tonometers, ICare and Tono-Pen Vet, before and each 5 minutes until 40 minutes later. The effects of other drugs were also studied at least with one-week interval. Based on the results of ICare tonometer, tetracaine significantly reduced the IOP immediately and 25 minutes after instillation. IOP changes after instillation of bupivacaine, lidocaine and proparacaine were not significant at any time compared to baseline values (p > 0.05). Based on the results of Tono-Pen Vet tonometer, all drugs reduced the IOP immediately after use; however, the effects of bupivacaine and lidocaine on IOP were much lower than that of tetracaine and proparacaine. The average duration of corneal anesthesia were 20, 15.5, 7.5 and 21 minutes for tetracaine, bupivacaine, lidocaine, and proparacaine, respectively. It is concluded that IOP reduction by local anesthetics when Tono-Pen Vet is used is much greater than the ICare tonometer measurements. Also, the reduction of IOP with each of the devices when tetracaine or proparacaine is used is greater than when bupivacaine or lidocaine is used.","PeriodicalId":36826,"journal":{"name":"Iranian Journal of Veterinary Science and Technology","volume":"12 1","pages":"47-54"},"PeriodicalIF":0.0,"publicationDate":"2020-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44686494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Management of Corneal Foreign Body in a Dog 1例犬角膜异物的处理
Q4 Veterinary Pub Date : 2020-01-09 DOI: 10.37591/RRJOVST.V8I3.2494
P. Kumar, V. Tejaswani
A nine-year-old male dog presented with blepharospasm and epiphora in the right eye was diagnosed to have a penetrating corneal foreign body. As the dog was non-cooperative, it was decided to remove the foreign body under general anesthesia. The ocular cul-de-sac was irrigated with 0.5% povidone iodine solution under general anesthesia and the foreign body was removed carefully using a fine needle tip. The cornea was checked for its intactness at the site of injury and found a small penetrating wound. Postoperative medication was given for seven postoperative days. The dog was able to close its eyelids partially by first operative day and the symptoms such as blepharospasm and epiphora were subsided by second postoperative day. Complete recovery was noticed by seventh postoperative day and no postoperative complications were recorded. Keywords: Blepharospasm, corneal foreign body, epiphora, surgical management, thorn Cite this Article V. Tejaswani, P. Ravi Kumar . Management of Corneal Foreign Body in a Dog Research & Reviews: Journal of Veterinary Science and Technology. 2019; 8( 3 ): 1 2 p.
一只九岁的公狗在右眼表现为眼睑痉挛和外显,被诊断为有穿透性角膜异物。由于狗不配合,我们决定在全身麻醉下取出异物。全麻下用0.5%聚维酮碘溶液冲洗眼囊死角,并用细针尖小心取出异物。检查受伤部位的角膜是否完好,发现一个小的穿透性伤口。术后用药7天。术后第一天能部分闭上眼睑,术后第2天眼睑痉挛、上睑下垂等症状消退。术后第7天完全恢复,无术后并发症记录。关键词:眼睑痉挛,角膜异物,上睑下垂,手术处理,刺犬角膜异物的处理研究与评述:中华兽医科学技术杂志。2019;8(3): 1 2 p。
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引用次数: 0
Management of Crop Foreign Body in a Juvenile Chicken (Gallus gallus domesticus) 雏鸡作物异物的处理
Q4 Veterinary Pub Date : 2020-01-09 DOI: 10.37591/RRJOVST.V8I3.2488
P. Kumar, V. Tejaswani
A one-month-old domestic chick (Gallus gallus domesticus) presented to the hospital with symptom of anorexia for the past one day was diagnosed to have a crop foreign body and decided for surgical correction. Under local analgesia, the crop was opened and the foreign body was removed. The ingluviotomy wound was closed by Cushing’s suture with size 3-0 Polyglactin 910 and skin wound by simple interrupted sutures using size 2-0 braided silk. The foreign body removed was a plastic tube of 8 cm length. Postoperatively, the bird was given antibiotics for three days. Regular dressing of the wound was done till the ninth postoperative day. Uneventful recovery was noticed with no postoperative complications. Keywords: Chick, crop foreign body, local analgesia, ingluviotomy, Cushing’s suture Cite this Article V. Tejaswani, P. Ravi Kumar. Management of Crop Foreign Body in a Juvenile Chicken (Gallus gallus Research & Reviews: Journal of Veterinary Science and Technology. 2019; 8(3): 6 8 p.
一只1个月大的家鸡(Gallus Gallus domesticus)在过去一天以厌食症状来到医院,被诊断为有农作物异物,决定进行手术矫正。局部镇痛下,切开异物,取出异物。开腹切口采用3-0号聚乳酸蛋白910库欣缝合,皮肤切口采用2-0号编织丝简单间断缝合。取出的异物是一根8厘米长的塑料管。术后,这只鸟服用了三天的抗生素。术后第9天常规包扎创面。术后无并发症,恢复平稳。关键词:鸡,作物异物,局部镇痛,肠切开,库欣缝合雏鸡(Gallus Gallus)作物异物的处理研究与评论:兽医科学与技术杂志。2019;8(3): 6。
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引用次数: 0
Bovine Mastitis and its Treatment Strategies 牛乳腺炎及其治疗策略
Q4 Veterinary Pub Date : 2019-03-07 DOI: 10.37591/RRJOVST.V7I3.1540
Rojita Yengkhom, Mokshta Gupta, E. Lokesha, B. Handique, L. Singh
Mastitis remains a major challenge to the dairy industry in terms of economic losses occurring throughout the world. Bovine mastitis is the endemic, persistent and inflammatory reaction of the mammary gland and/or udder tissue of milch animals particularly medium to high yielding dairy cattle, usually in and around peri-parturient period caused by various infectious or non-infectious etiological agents. It is a global problem as it affects animal health, milk production as well economics of the country. The clinical signs and diagnosis may vary depending on the source, mode of the transmission and forms of the disease. Several methods with varying results exist for the management of different forms of bovine mastitis. The objective of this present review is to describe the relevant aspects of physiology, clinical signs, diagnosis, and treatment strategies of bovine mastitis. Keywords : Bovine, mastitis, mammary gland, health Cite this Article Yengkhom R, Gupta M, Lokesha E, et al. Bovine Mastitis and Its Treatment Strategies. Research & Reviews: Journal of Veterinary Science and Technology. 2018; 7(3): 11–16p.
乳腺炎仍然是一个主要的挑战,乳品行业的经济损失发生在世界各地。牛乳腺炎是一种地方性的、持续性的炎症反应,发生在哺乳动物,特别是中高产奶牛的乳腺和/或乳房组织,通常发生在围产期和前后,由各种传染性或非传染性病原体引起。这是一个全球性问题,因为它影响动物健康、牛奶生产以及国家经济。临床症状和诊断可能因疾病的来源、传播方式和形式而异。几种方法与不同的结果存在管理不同形式的牛乳腺炎。本综述的目的是描述有关方面的生理学,临床体征,诊断和治疗策略的牛乳腺炎。关键词:牛,乳腺炎,乳腺,健康Yengkhom R, Gupta M, Lokesha E等牛乳腺炎及其治疗策略。研究综述:《兽医科技杂志》2018;7 (3): 11-16p。
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引用次数: 2
期刊
Iranian Journal of Veterinary Science and Technology
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