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Relationship between fructusamine, glucose, total protein, and albumin concentrations of serum in late pregnancy and early lactation of dairy Saanen goat 奶山羊妊娠晚期和泌乳早期血清果糖胺、葡萄糖、总蛋白和白蛋白浓度的关系
Q4 Veterinary Pub Date : 2018-09-12 DOI: 10.22067/VETERINARY.V10I1.64963
R. Pourmohammad, M. Mohri, H. Seifi
The changes in serum fructosamine concentration of dairy Saanen goats during late pregnancy and early lactation and its relationship to serum glucose, total protein, and albumin concentrations was investigated. Eleven Saanen goats were selected for the study. Blood samples were collected by the jugular vein in 30, 15 and 7 days before the expected time of parturition (D-30, D-15, and D-7, respectively) and also 12 hours and 3, 7, 13, and 42 days post-partum (H+12, D+3, D+7, D+13, and D+42, respectively). The serum concentrations of fructosamine, glucose, albumin, and total protein were measured. The maximum concentration of fructosamine was at 12 hours post-partum and decreased thereafter. Serum concentration of glucose significantly increased from D-15 up to 12 hours post-partum and then decreased and stayed at the same level. The serum concentrations of albumin and total protein significantly increased during post-partum period. There were significant correlations between fructosamine and glucose concentrations at post-partum period and overall time of the study. Linear regression analysis between each sampling amount of glucose and three consecutive fructosamine concentrations revealed significant positive correlation between glucose of each sampling time with the fructosamine of first sampling time thereafter. According to the correlation between glucose and fructosamine amounts during the study, especially post-partum period in Saanen goat, fructosamine measurement could be used as a useful indicator of energy economy and probably stress in Sannen goat; but controversy between reports in ruminants needs further studies for better understanding of fructosamine as energy biomarker in ruminants.
研究了萨能山羊妊娠晚期和泌乳早期血清果糖胺浓度的变化及其与血糖、总蛋白和白蛋白浓度的关系。选择了11只萨能山羊进行研究。在预期分娩时间(分别为D-30、D-15和D-7)前30、15和7天,以及产后12小时和3、7、13和42天(分别为H+12、D+3、D+7、D+13和D+42),通过颈静脉采集血样。测定血清果糖胺、葡萄糖、白蛋白和总蛋白的浓度。果糖胺的最高浓度出现在产后12小时,此后有所下降。从D-15到产后12小时,血清葡萄糖浓度显著升高,然后下降并保持在相同水平。血清白蛋白和总蛋白浓度在产后显著升高。果糖胺和产后血糖浓度以及研究的总时间之间存在显著相关性。每次葡萄糖采样量与连续三次果糖胺浓度之间的线性回归分析显示,每次采样时间的葡萄糖与此后第一次采样时间的果糖胺之间存在显著的正相关。根据研究期间,特别是萨能山羊产后期间葡萄糖和果糖胺含量之间的相关性,果糖胺测量可以作为萨能山羊能量经济性和可能的压力的有用指标;但关于反刍动物的报道之间存在争议,需要进一步研究以更好地理解果糖胺作为反刍动物能量生物标志物。
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引用次数: 1
Molecular characterization of some virulence genes of Salmonella enterica serotype sandow and saintpaul isolated from environment of dairy farms at Assiut Province, Egypt 埃及Assiut省奶牛场环境中分离的肠炎沙门氏菌sandow和saintpaul血清型部分毒力基因的分子特征
Q4 Veterinary Pub Date : 2018-07-19 DOI: 10.4172/2157-7579.1000559
E. Sedeek, S. Sedeek, S. Sotohy
Dairy farm environment could become contaminated with Salmonella following outbreaks of illness, colonization of animals or by general contamination. The present study investigated a total number of 95 samples collected from 3 dairy farms in 3 different localities at Assiut Province including air (25), water (35) and manure (35) samples for detection of Salmonella species by bacteriological isolation, morphological, biochemical and serological identification followed by molecular characterization for the presence of 6 virulence genes; pefA (700 bp), mgtC (677 bp), stn (617 bp), sopB (517 bp), invA (284 bp) and avrA (422 bp) in Salmonella isolates. Bacteriological examination revealed the isolation of 3 (3.2%) Salmonella isolates from totally examined samples, which were identified serologically as 1 (2.9%) Salmonella enterica serotype Sandow isolated from water and 2 Salmonella enterica serotype Saintpaul from air and manure (one from each with 2.9% of each. Molecular characterization of the 3 Salmonella isolates revealed that all 6 tested virulence genes; except sopB (517 bp); presented in Salmonella enterica serotype Sandow isolated from water, while all 6 tested virulence genes found in Salmonella enterica serotype Saintpaul isolated from manure and found that invA (284 bp) was the only virulence gene located in Salmonella enterica serotype Saintpaul isolated from air. This study highlighted on the potential sources for Salmonella contamination moreover the epidemiology of salmonellosis in dairy farms that necessitate following of strict healthful measures to reduce the risk of Salmonella infection that still constitutes a significant world zoonosis particularly through contamination of dairy farm environments that contribute to extend the recycling of Salmonella that considered the most vital sources of animal and human infection with Salmonella.
在疾病爆发、动物定植或一般污染后,奶牛场环境可能被沙门氏菌污染。本研究从阿西尤特省3个不同地区的3个奶牛场采集95份样本,包括空气(25份)、水(35份)和粪便(35份)样本,通过细菌学分离、形态学、生化和血清学鉴定,并对6个毒力基因进行分子鉴定,对沙门氏菌种类进行检测;沙门氏菌分离物中pefA (700 bp)、mgtC (677 bp)、stn (617 bp)、sopB (517 bp)、invA (284 bp)和avrA (422 bp)的表达量。细菌学检查共检出沙门氏菌3株(3.2%),其中从水中分离出桑多沙门氏菌1株(2.9%),从空气和粪便分离出圣保罗沙门氏菌2株(各1株,各2.9%)。3株沙门氏菌的分子鉴定结果显示,6个毒力基因均检测到;除了sopB (517 bp);从粪便中分离出的6个大肠沙门氏菌毒力基因均存在,空气中分离出的大肠沙门氏菌毒力基因中只存在invA (284bp)。这项研究强调了沙门氏菌污染的潜在来源,以及奶牛场沙门氏菌病的流行病学,需要遵循严格的健康措施来降低沙门氏菌感染的风险,沙门氏菌感染仍然是世界上重要的人畜共患病,特别是通过污染奶牛场环境,有助于扩大沙门氏菌的回收利用,沙门氏菌被认为是动物和人类感染沙门氏菌的最重要来源。
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引用次数: 2
Effect of lemon (Citrus lemon) pumace powder supplementation on growth performance, lipid peroxidation and protein oxidation biomarkers in some tissues of common carp (Cyprinus carpio) 添加柠檬(Citrus lemon)浮石粉对鲤鱼生长性能、部分组织脂质过氧化和蛋白质氧化生物标志物的影响
Q4 Veterinary Pub Date : 2018-07-15 DOI: 10.22067/veterinary.v1-2i10-11.73469
ُSara Safaeian Laein, A. Salari, D. Shahsavani, H. Baghishani
The aim of this study was to evaluate the antioxidant potential of lemon pumace powder (peel and pulp), as an inexpensive and valuable source of nutrient in diet of common carp. Fish were divided randomly into four groups of 30 each. Group 1 fish were fed with basic diet, serving as the control. Fish in group 2 and 3 and 4 were fed the basic diet supplemented with 1.5%, 3% and 5% lemon pumace powder, respectively. Results showed that growth performance including final weight, weight gain (WG), feed conversion ratio (FCR) and specific growth rate (SGR) increased significantly as compared to control. Malondialdehyde (MDA) values of muscle increased significantly as compared to control in all treatment groups and the decreeing effect of lemon pumace powder on malondialdehyde (MDA) values of kidney and liver was only significant in group 4, when compared with the control group (P<0.05). Protein carbonyl contents were decreased significantly in kidney and liver in group 3 and 4 as compared to control group and Protein carbonyl of muscle decreased significantly as compared to control in all treatment groups. FRAP values of liver increased significantly only in group 3 as compare to control and FRAP values of kidney and muscle increased significantly only in group 4 as compared to control (P<0.05). These data suggest that supplementation of 5% lemon pumace powder to be more effective than lower levels in strengthening the antioxidant system against oxidative stress.
本研究的目的是评估柠檬粉(果皮和果肉)的抗氧化潜力,柠檬粉是鲤鱼日粮中一种廉价而有价值的营养来源。鱼被随机分为四组,每组30只。第1组采用基础日粮喂养,作为对照。第2组、第3组和第4组的鱼分别饲喂添加1.5%、3%和5%柠檬粉的基础日粮。结果表明,与对照相比,生长性能(包括终重、增重(WG)、饲料转化率(FCR)和比生长率(SGR))显著提高。各治疗组肌肉丙二醛(MDA)值均较对照组显著升高,而柠檬粉对肾脏和肝脏丙二醛(丙二醛)值的降低作用仅在第4组显著,与对照组相比(P<0.05),第3组和第4组肾脏和肝脏中的蛋白质羰基含量均比对照组显著降低,肌肉中的蛋白质羰基比对照组明显降低。与对照组相比,只有第3组的肝脏FRAP值显著增加,只有第4组的肾脏和肌肉FRAP值明显增加(P<0.05)。这些数据表明,在增强抗氧化系统对抗氧化应激方面,补充5%柠檬粉比低水平柠檬粉更有效。
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引用次数: 5
Biomechanical study in vitro on the use of self-designed external fixator in diaphyseal III metacarpal fractures in horses 自行设计外固定架治疗马骨干III型掌骨骨折的体外生物力学研究
Q4 Veterinary Pub Date : 2018-05-30 DOI: 10.4172/2157-7579-C1-036
B. Turek, A. Potyński, C. Wajler, T. Szara, M. Czopowicz, O. Drewnowska
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引用次数: 0
Current and future delivery models for the provision of OV services in the meat industry in Great Britain 英国肉类行业提供OV服务的当前和未来交付模式
Q4 Veterinary Pub Date : 2018-05-30 DOI: 10.4172/2157-7579-C1-035
pLewis G Grantp
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引用次数: 0
Hematological Profile of Sheep Experimentally Infested with Bovicola ovis 绵羊羊乳弧菌实验感染的血液学特征
Q4 Veterinary Pub Date : 2018-04-23 DOI: 10.4172/2157-7579.1000536
A. Tadie, G. Terefe, Yonas Gizaw
Changes in some haemotological parameters (erythrocyte count, hematocrit values and leukocyte counts) in animals with lice infestation had been reported previously. In this regards, information on the effect of Bovicola ovis (B. ovis) infestation on hematological values under experimental condition in Ethiopian sheep is not available. A total of 12 animals divided into two groups of six animals each were used in the experiment. Animals in one group were infested with 500 lice (Bovicola ovis) per sheep collected from different donor animals and the other group served as a non-infested control. Each group was observed for a period of eight consecutive weeks. The present findings demonstrated that the mean parasite burden/10 cm2 of skin was 16 in infested group whereas the control group did not have any lice. Packed cell volume was not significantly different between the two groups and values were in the normal range. On the other hand total RBC count was significantly lower for the infested group (P<0.05). Comparison of the leukocyte counts between the two groups indicated that the total count and eosinophil levels were significantly elevated in the infested group (P<0.05). In conclusion, B. ovis infestation results not only skin damage but also causes systemic changes that may significantly alter the physiological and health status of infested animals.
一些血液学参数的变化(红细胞计数、红细胞压积值和白细胞计数)在虱子感染的动物中已有报道。在这方面,没有关于在实验条件下埃塞俄比亚羊的Bovicola ovis (B. ovis)侵染对血液学值影响的信息。12只动物被分成两组,每组6只。一组动物每只从不同供体动物收集的羊感染500只虱子,另一组作为未感染的对照。每组连续观察8周。结果表明,感染组皮肤平均寄生虫负荷为16只/10 cm2,而对照组皮肤无虱子。两组间堆积细胞体积无显著差异,均在正常范围内。另一方面,侵染组红细胞总数显著低于侵染组(P<0.05)。两组白细胞计数比较,侵染组白细胞总数和嗜酸性粒细胞水平显著升高(P<0.05)。综上所述,卵圆杆菌侵染不仅会造成皮肤损伤,还会引起全身的改变,从而显著改变被侵染动物的生理和健康状况。
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引用次数: 2
Management of Burns in Companion Animals: A Report of Three Cases 伴侣动物烧伤的处理:附三例报告
Q4 Veterinary Pub Date : 2018-04-21 DOI: 10.37591/rrjovst.v5i3.555
V. Prasad, P. Kumar, M. Sreenu, N. Harikrishna
Abstract Three cases of burns in a dog, monkey and an emu bird were reported. The dog had a deep partial thickness burn, on the anterolateral aspect of right hind limb and a superficial burn on the tip of the glans penis. The baby monkey had a deep and full thickness burn on the abaxial surface of the left palm. The emu bird had a partial thickness burn. They were treated successfully with fluid therapy, antibiotics, opiates and silver sulphadiazine ointment. Keywords: Burns, antiseptic ointment, partial thickness burns, full thickness burns Cite this Article Devi Prasad V, Ravi Kumar P, Makkena Sreenu et al. Management of Burns in Companion Animals: A Report of Three Cases. Research & Reviews: Journal of Veterinary Science and Technology. 2016; 5(3): 21–24p.
摘要报告了3例犬、猴和鸸鹋鸟烧伤病例。犬右后肢前外侧有深度部分厚度烧伤,阴茎头尖端有浅表烧伤。这只小猴的左手掌背面有一处深而厚的烧伤。鸸鹋鸟有部分厚度烧伤。他们通过液体疗法、抗生素、阿片类药物和磺胺嘧啶银软膏成功治疗。关键词:烧伤,抗菌软膏,局部烧伤,全层烧伤伴侣动物烧伤的处理:附三例报告。研究综述:Journal of Veterinary Science and Technology. 2016;5 (3): 21-24p。
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引用次数: 2
Dystocia in Nellore Brown Ewe Due to Breech Presentation of Twin Lamb 双羔臀位致内洛褐母羊难产
Q4 Veterinary Pub Date : 2018-04-21 DOI: 10.37591/RRJOVST.V4I1.536
K. Jyothi, Y. P. Reddy, K. S. Gunaranjan, M. Rao
A Nellore Brown ewe in its first parity was presented to the clinic with a complaint of abdominal straining. Previous day it delivered a live male lamb. Abdominal palpation revealed presence of another lamb. Per vaginal examination revealed breech presentation of lamb. Both rear legs brought into birth canal and a dead female lamb removed with gentle traction. Prolonged dystocia may lead to death of lamb and economical losses, if presented early the lamb losses can be lowered. Keywords: Breech presentation, traction, dystocia, nellore brown, fetal Cite this Article K. Jyothi, Y.V. Pridhvidhar Reddy, K. Sai Gunaranjan, et al. Dystocia in Nellore Brown Ewe Due to Breech Presentation of Twin Lamb. Research & Reviews: Journal of Veterinary Science and Technology. 2015; 4(1): 14–16p.
一个内洛尔布朗母羊在它的第一个胎是提出了腹部紧张的投诉到诊所。前一天,它接生了一只活公羊羔。腹部触诊发现有另一只羔羊。阴道检查显示羔羊臀位。两条后腿被带进产道,一只死去的母羊羔被轻轻地牵引走了。长时间难产可导致羔羊死亡和经济损失,及早发现可降低羔羊损失。关键词:臀位,牵引,难产,内褐,胎儿引用本文K. Jyothi, Y.V. Pridhvidhar Reddy, K. Sai Gunaranjan等双羔臀位致内洛褐母羊难产。研究综述:《兽医科技》杂志2015;4 (1): 14-16p。
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引用次数: 0
Application of Nutrigenomics for Enhancement of Body Growth in Ruminants 营养基因组学在促进反刍动物身体生长中的应用
Q4 Veterinary Pub Date : 2018-04-21 DOI: 10.37591/RRJOVST.V5I1.514
M. Mondal, M. Ghosh
the same diet may perform differently and it is due to differences in ability of nutrients to interact within the biological systems affecting molecular pathways resulting in differences in body weight gain, production or reproduction performances. For better understanding of the above processes, nutrigenomics and endocrinomics; two emerging fields that are of great help to discover the differential expression and function of genes occur with different diets, have come up. Nutrigenomics is dealt with the effects of each and every bioactive chemical in foods and supplements that alter gene expression in an animal. On the other hand, nutrients alter the hormonal inter milieu of the animal thereby ‘turn on’ or ‘turn off’ of a particular set of pathways affecting ‘switching on’ or switching off’ of a defined set of genes (Endocrinomics). Hence, there is always a crosstalk between nutrigenomics and endocrino-genomics. This paper highlights the importance of nutrigenomics and endocrinomics, and their interactions during the process of growth in domestic animals in general and bovine species in particular. Keywords: Endocrine genomics, transcriptome, microarray, nutritional genomics, diet-gene interaction Cite this Article Mondal M., Ghosh M.K. Application of nutrigenomics for enhancement of body growth in ruminants. Research & Reviews: Journal of Veterinary Science and Technology. 2016; 5(1): 1–6p.
同样的饮食可能表现不同,这是由于营养物质在影响分子途径的生物系统内相互作用的能力不同,从而导致体重增加、生产或繁殖性能的差异。为了更好地理解上述过程,营养基因组学和内分泌学;出现了两个新兴领域,它们对发现不同饮食中基因的差异表达和功能有很大帮助。营养基因组学研究的是食物和补充剂中每一种生物活性化学物质对动物基因表达的影响。另一方面,营养物质会改变动物体内的激素环境,从而“开启”或“关闭”一组特定的通路,影响一组特定基因的“开启”或“关闭”(内分泌学)。因此,营养基因组学和内分泌基因组学之间总是存在着一种串扰。本文强调了营养基因组学和内分泌组学的重要性,以及它们在家畜生长过程中的相互作用,特别是牛。关键词:内分泌基因组学,转录组,微阵列,营养基因组学,饮食-基因相互作用引用本文Mondal M, Ghosh M.K.营养基因组学在促进反刍动物身体生长中的应用。研究综述:Journal of Veterinary Science and Technology. 2016;5 (1): 1-6p。
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引用次数: 5
Designing Mithun Specific Probes Towards Development of Microarray for Mithun (Bos frontalis) 面向Mithun (Bos frontalis)微阵列的Mithun特异性探针设计
Q4 Veterinary Pub Date : 2018-04-21 DOI: 10.37591/rrjovst.v4i3.567
M. Mondal, M. Karunakaran, K. K. Baruah
cDNA microarrays are powerful tools to analyze the expression pattern of thousands of genes simultaneously in response to treatments, during tissue differentiation or related to differences in traits. To get accurate results, the probes used in the microarray are to be specific to the species for which the array is being conducted. Non-availability of suitable microarray specific to mithun (Bos frontalis), a rare ruminant of Southeast Asia, limits its use to study the expression patterns of genes globally. Therefore, the authors designed the probes and developed a mithun-specific microarray for the first time. Transcripts obtained from bovine genome array in mithun tissues and from BLAST search were used to design probes and subsequent development of mithun-specific microarray. A total of 3,35,576 numbers of transcripts obtained from the next generation sequencing (through microarray using bovine genome array chips) of mithun tissues including several hundreds of unknown genes and others obtained for BLAST search (11,865) were used for designing the probes towards development of mithun-specific microarrays. A total of 3,47,441 probes were designed and length of each probe was 60 bp. The developed array has total features of 4,20,288, of which 4,16,505 features are readily available in the developed array. Besides, there are 3783 numbers of controls in the array to obtain high performance. In conclusion, the authors developed mithun-specific microarray using probes that are specific to this species for the first time. This microarray for mithun contains fully defined set of genes suitable for detailed analysis of expression of many genes involved in this species. The developed microarray will be of great use to analyze the molecular mechanisms controlling cell proliferation, differentiation in response to different treatments or variation in economic traits such as marbling and disease resistance in mithun. Keywords: Mithun, microarray, cDNA, transcripts, expression, genes Cite this Article Mondal M, Karunakaran M, Baruah KK. Designing mithun specific probes towards development of microarray for mithun (Bos frontalis). Research & Reviews: Journal of Veterinary Science and Technology. 2015; 4(3): 1–7p.
cDNA微阵列是一种强大的工具,可以同时分析数千个基因对治疗、组织分化或性状差异的表达模式。为了得到准确的结果,微阵列中使用的探针必须是特定于该阵列正在进行的物种的。东南亚稀有反刍动物mithun (Bos frontalis)缺乏合适的基因芯片,限制了其在全球范围内研究基因表达模式的应用。因此,作者首次设计了探针并开发了一种微阵列。从牛micthun组织基因组阵列和BLAST搜索中获得的转录本用于设计探针和随后开发micthun特异性微阵列。从下一代测序(使用牛基因组阵列芯片)中获得的mithun组织转录本总数为3,35,576个,其中包括数百个未知基因和BLAST搜索获得的其他基因(11,865个),用于设计用于开发mithun特异性微阵列的探针。共设计探针3,47,441个,每个探针长度为60 bp。所开发的阵列具有4,20,288个特征,其中4,16,505个特征在所开发的阵列中随时可用。此外,阵列中有3783个控件,以获得高性能。总之,作者首次使用针对该物种的探针开发了针对该物种的微阵列。该微阵列包含完整定义的基因集,适合于详细分析该物种中涉及的许多基因的表达。所开发的微阵列将用于分析水稻在不同处理下细胞增殖、分化的分子机制,以及大理石花纹和抗病等经济性状的变化。关键词:Mithun,芯片,cDNA,转录本,表达,基因引用本文Mondal M, Karunakaran M, Baruah KK设计微管专用探针,开发微阵列用于微管(frontalis)。研究综述:《兽医科技》杂志2015;4 (3): 1-7p。
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引用次数: 0
期刊
Iranian Journal of Veterinary Science and Technology
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