Pub Date : 2018-04-21DOI: 10.37591/RRJOVST.V4I3.572
M. Mondal, K. Baruah
Non-esterified fatty acid (NEFA) is one of the most important blood metabolites contributing significantly during body growth, cyclicity, pregnancy and lactation, and it plays more vital role particularly during transition phase of the biological processes like pregnancy to lactation transition period. Determination of blood concentrations of NEFA is, therefore, important. The measurement technique or assay for measurement of blood NEFA should be simple, reliable, accurate, rapid and cheap. Here, the authors developed a sensitive and fast microtiterplate colorimetry-based method for estimation of NEFA in bovine plasma. The method is simple to perform and gives accurate, precise results in 100 µL samples. The developed method requires at least five times less quantity of plasma and reagents than that of already existing methods. Furthermore, ability of the method for taking reading of all the samples together on microtiterplate in a reader enable us to save a lot of time. Hence, the developed method may be adopted for estimation of NEFA in bovine plasma. Keywords: NEFA, bovine, microtiterplate, plasma, assay Cite this Article Mondal M, Baruah KK. Development of A Rapid Microtiterplate Based Colorimetric Method for Estimation of Non-Esterified Fatty Acids in Bovine Plasma. Research & Reviews: Journal of Veterinary Science and Technology . 2015; 4(3): 29–34p.
{"title":"Development of A Rapid Microtiterplate Based Colorimetric Method for Estimation of Non-esterified Fatty Acids in Bovine Plasma","authors":"M. Mondal, K. Baruah","doi":"10.37591/RRJOVST.V4I3.572","DOIUrl":"https://doi.org/10.37591/RRJOVST.V4I3.572","url":null,"abstract":"Non-esterified fatty acid (NEFA) is one of the most important blood metabolites contributing significantly during body growth, cyclicity, pregnancy and lactation, and it plays more vital role particularly during transition phase of the biological processes like pregnancy to lactation transition period. Determination of blood concentrations of NEFA is, therefore, important. The measurement technique or assay for measurement of blood NEFA should be simple, reliable, accurate, rapid and cheap. Here, the authors developed a sensitive and fast microtiterplate colorimetry-based method for estimation of NEFA in bovine plasma. The method is simple to perform and gives accurate, precise results in 100 µL samples. The developed method requires at least five times less quantity of plasma and reagents than that of already existing methods. Furthermore, ability of the method for taking reading of all the samples together on microtiterplate in a reader enable us to save a lot of time. Hence, the developed method may be adopted for estimation of NEFA in bovine plasma. Keywords: NEFA, bovine, microtiterplate, plasma, assay Cite this Article Mondal M, Baruah KK. Development of A Rapid Microtiterplate Based Colorimetric Method for Estimation of Non-Esterified Fatty Acids in Bovine Plasma. Research & Reviews: Journal of Veterinary Science and Technology . 2015; 4(3): 29–34p.","PeriodicalId":36826,"journal":{"name":"Iranian Journal of Veterinary Science and Technology","volume":"34 1","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"2018-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75867043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-21DOI: 10.37591/RRJOVST.V6I2.563
V. Prasad, P. Kumar, M. Sreenu
Pyometra is a frequently diagnosed bacterial infection of the uterus in intact, sexually mature bitches, leading to the accumulation of purulent material in the uterine lumen. Approximately one third of the anoestrous bitches can be diagnosed with pyometra. Closed-cervix pyometra is particularly dangerous, because septicaemia and toxaemia may develop rapidly and if left untreated can result in death of the patient. Due to the insidious nature of the disease and its sometimes equivocal clinical signs, patients are often presented in poor condition for anaesthesia and surgery. Keywords: Pyometra, uterus, bitches Cite this Article Prasad VD, Kumar PR, Sreenu M. Pyometra in Bitches: A Review of Literature. Research & Reviews: Journal of Veterinary Science and Technology. 2017; 6(2): 12–20p.
{"title":"Pyometra in Bitches: A Review of Literature","authors":"V. Prasad, P. Kumar, M. Sreenu","doi":"10.37591/RRJOVST.V6I2.563","DOIUrl":"https://doi.org/10.37591/RRJOVST.V6I2.563","url":null,"abstract":"Pyometra is a frequently diagnosed bacterial infection of the uterus in intact, sexually mature bitches, leading to the accumulation of purulent material in the uterine lumen. Approximately one third of the anoestrous bitches can be diagnosed with pyometra. Closed-cervix pyometra is particularly dangerous, because septicaemia and toxaemia may develop rapidly and if left untreated can result in death of the patient. Due to the insidious nature of the disease and its sometimes equivocal clinical signs, patients are often presented in poor condition for anaesthesia and surgery. Keywords: Pyometra, uterus, bitches Cite this Article Prasad VD, Kumar PR, Sreenu M. Pyometra in Bitches: A Review of Literature. Research & Reviews: Journal of Veterinary Science and Technology. 2017; 6(2): 12–20p.","PeriodicalId":36826,"journal":{"name":"Iranian Journal of Veterinary Science and Technology","volume":"1 1","pages":"12-20"},"PeriodicalIF":0.0,"publicationDate":"2018-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89701477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-21DOI: 10.37591/RRJOVST.V4I3.568
P. Birteeb, saac O Tetteh, A. Salifu
The study was carried out on 24 neonatal large white piglets at the National Livestock Breeding Station, Pong-Tamale to assess their performance when weaned at different ages and to determine the relationship between linear body measurements and the body weight of piglets in weight prediction. The piglets were randomly weaned at three selected weaning ages (4, 6 and 8 weeks) but reared under uniform conditions. Initial body weight (BW), initial body length (BL), initial chest girth (CG) and initial height at withers (HW); were measured. Weekly measurements of the parameters were taken for 20 weeks. By the 20th week, piglets weaned at 8 weeks were superior (P 0.05) were recorded in the body length and the height at withers of the selected weaned ages. The chest girth was the best predictor of body weight, yielding highest prediction accuracies of 92.3% (at 5 weeks), 76.9% (at 10 weeks), 86.1% (at 15 weeks) and 87.5% (at 20 weeks). Conclusively, large white piglets grew better when weaned at 8 weeks, and chest girth could be reliably used to predict body weight in large white grower pigs. Keywords : Swine, body measurements, linear, livestock, prediction Cite this Article Birteeb Peter T, Tetteh Isaac O, Salifu Abdul-Rahaman S. Growth Perfor-mance and Weight Estimation of Large White Piglets Weaned at Different Ages. Research & Reviews: Journal of Veterinary Science and Technology . 2015; 4(3): 15–23p.
本研究以24头新生大白仔猪为试验对象，评估其不同日龄断奶时的生产性能，并确定体重预测中线性体重测量与仔猪体重之间的关系。仔猪在3个断奶日龄(4、6和8周)随机断奶，但饲养条件相同。初始体重(BW)、初始体长(BL)、初始胸围(CG)和初始肩高(HW);被测量。每周测量参数，持续20周。到第20周时，8周龄断奶仔猪的体长和舍马高度均优于其他断奶仔猪(P < 0.05)。胸围是体重的最佳预测指标，预测准确率最高，为92.3%(5周)、76.9%(10周)、86.1%(15周)和87.5%(20周)。综上所述，大白猪在8周断奶时生长较好，胸围可以可靠地预测大白猪的体重。关键词:猪，体测量，线性，家畜，预测引用本文Birteeb Peter T, Tetteh Isaac O, Salifu Abdul-Rahaman s不同日龄断奶大白仔猪生长性能及体重估算研究与评述:兽医科学与技术杂志。2015;4 (3): 15-23p。
{"title":"Growth Performance and Weight Estimation of Large White Piglets Weaned at Different Ages","authors":"P. Birteeb, saac O Tetteh, A. Salifu","doi":"10.37591/RRJOVST.V4I3.568","DOIUrl":"https://doi.org/10.37591/RRJOVST.V4I3.568","url":null,"abstract":"The study was carried out on 24 neonatal large white piglets at the National Livestock Breeding Station, Pong-Tamale to assess their performance when weaned at different ages and to determine the relationship between linear body measurements and the body weight of piglets in weight prediction. The piglets were randomly weaned at three selected weaning ages (4, 6 and 8 weeks) but reared under uniform conditions. Initial body weight (BW), initial body length (BL), initial chest girth (CG) and initial height at withers (HW); were measured. Weekly measurements of the parameters were taken for 20 weeks. By the 20th week, piglets weaned at 8 weeks were superior (P 0.05) were recorded in the body length and the height at withers of the selected weaned ages. The chest girth was the best predictor of body weight, yielding highest prediction accuracies of 92.3% (at 5 weeks), 76.9% (at 10 weeks), 86.1% (at 15 weeks) and 87.5% (at 20 weeks). Conclusively, large white piglets grew better when weaned at 8 weeks, and chest girth could be reliably used to predict body weight in large white grower pigs. Keywords : Swine, body measurements, linear, livestock, prediction Cite this Article Birteeb Peter T, Tetteh Isaac O, Salifu Abdul-Rahaman S. Growth Perfor-mance and Weight Estimation of Large White Piglets Weaned at Different Ages. Research & Reviews: Journal of Veterinary Science and Technology . 2015; 4(3): 15–23p.","PeriodicalId":36826,"journal":{"name":"Iranian Journal of Veterinary Science and Technology","volume":"32 1","pages":"15-23"},"PeriodicalIF":0.0,"publicationDate":"2018-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78475304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-21DOI: 10.37591/RRJOVST.V5I1.519
N. Kharayat, G. Chaudhary, R. Katiyar, B. Balmurugan, M. Patel, S. Uniyal, M. Raza, G. Mishra
Abstract Artificial insemination (AI) involves the deposition of semen into female reproductive tract manually. It starts from the collection of the semen from the male and its evaluation in terms of motility, viability and concentration followed by its deposition into female reproductive tract. Sexual maturity in both male and female bird occurs at 18 weeks of age. One ejaculate of a male can cover up 20 female birds by using AI. Dose of semen is 100–200 million spermatozoa/insemination in 50 microliter volume. Poultry semen shows poor response for cryopreservation so AI is done as soon as semen is collected. Further research is needed regarding poultry sperm biology, cellular and molecular basis of oviductal spermatozoa transport, selection, and storage to make AI more efficient in the birds. Keywords: Artificial insemination, semen, cryopreservation Cite this Article Kharayat NS, Chaudhary GR, Katiyar R et al. Significance of Artificial Insemination in Poultry. Research & Reviews: Journal of Veterinary Science and Technology. 2016; 5(1): 15–19p
摘要人工授精是指人工将精液注入女性生殖道。它从收集男性精液开始，并根据其活力，活力和浓度进行评估，然后将其沉积到女性生殖道。雄鸟和雌鸟的性成熟发生在18周龄。一只雄鸟的一次射精可以用人工智能覆盖20只雌鸟。精液剂量为50微升体积1 - 2亿个精子/人工授精。家禽精液对低温保存反应较差，因此采集精液后立即进行人工受精。需要进一步研究家禽精子生物学，输卵管精子运输、选择和储存的细胞和分子基础，以提高鸟类人工智能的效率。关键词:人工授精，精液，冷冻保存家禽人工授精的意义。研究综述:Journal of Veterinary Science and Technology. 2016;5 (1): 15-19p
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Pub Date : 2018-04-21DOI: 10.37591/rrjovst.v5i2.529
S. Uniyal, A. Mishra, K. Aswin, J. K. Sahoo, V. Munde, G. Mishra
Abstract Methane (CH4) and other gases like carbon dioxide (CO2), nitrous oxide (N2O), are green house gases (GHGs) that aggravates the effects of solar and thermal radiation on surface and atmospheric temperature. CH4 is the second largest GHG having 21 times more heat generation potential than CO2. Global livestock agriculture was responsible for 18% of the anthropogenic GHG emissions annually. CH4 production in the rumen accounts for 2–12 % loss of gross energy and consequently influence performance of ruminants. So, reducing ruminant CH4 emissions is an important objective for ensuring the sustainability of ruminant-based livestock farming. CH4 is formed in the rumen by methanogens (part of the domain Archaea), mainly from H2 and CO2. Within the rumen microbial food web, methanogens perform the beneficial task of removing H2, that allows reduced cofactors to be reoxidized and recycled, thereby enhancing the breakdown and fermentation of plant material. Therefore, rumen CH4 mitigation strategies need to consider alternative routes of H2 utilization. Nitrates and sulphates are potent inhibitors of CH4 in many anaerobic system including rumen; these salts have greater affinity for H2 as compared to CO2 resulting in net reduction in CH4 production. Experimental evidence suggest that nitrate and sulphate inhibit CH4 production both in vitro and in vivo without any adverse effect on rumen fermentation but the drawback is that nitrate is toxic to the animals; so supplementation at lower level helps the animal to acclimatize and reduces the chances of toxicity Keywords: Methane (CH4), sulphates, hydrogen, methanogens, fermentation Cite this Article Uniyal S, Mishra AK, Aswin K, et al. Use of Nitrates and Sulphates as Hydrogen Sink in Reducing Enteric Methane Production. Research & Reviews: Journal of Veterinary Science and Technology. 2016; 5(2): 5–8p.
甲烷(CH4)和二氧化碳(CO2)、氧化亚氮(N2O)等气体是温室气体(ghg)，它们加剧了太阳和热辐射对地表和大气温度的影响。甲烷是第二大温室气体，其产生热量的潜力是二氧化碳的21倍。全球畜牧业每年为18%的人为温室气体排放负责。瘤胃CH4的产生占总能量损失的2 - 12%，影响反刍动物的生产性能。因此，减少反刍动物甲烷排放是确保以反刍动物为基础的畜牧业可持续发展的重要目标。CH4在瘤胃中由产甲烷菌(古菌域的一部分)生成，主要由H2和CO2生成。在瘤胃微生物食物网中，产甲烷菌执行去除H2的有益任务，使还原的辅因子被再氧化和循环，从而促进植物物质的分解和发酵。因此，瘤胃CH4缓解策略需要考虑H2利用的替代途径。硝酸盐和硫酸盐在包括瘤胃在内的许多厌氧系统中是CH4的有效抑制剂;与二氧化碳相比，这些盐对H2有更大的亲和力，导致CH4产量的净减少。实验表明，硝酸盐和硫酸盐对体外和体内CH4的产生均有抑制作用，对瘤胃发酵无不良影响，但硝酸盐对动物有毒性;因此，较低水平的补充有助于动物适应环境并减少毒性的机会关键词:甲烷(CH4)，硫酸盐，氢气，产甲烷菌，发酵引用本文unial S, Mishra AK, Aswin K，等。硝酸盐和硫酸盐作为氢汇在减少肠道甲烷生成中的应用。研究综述:Journal of Veterinary Science and Technology. 2016;5 (2): 5-8p。
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Pub Date : 2018-04-21DOI: 10.37591/RRJOVST.V4I3.573
M. Mondal, M. Karunakaran, M. Ghosh, T. K. Dutta
Declined fertility of the high-yielding dairy cows reared under intensive condition of management is of great concern not only for India but for the whole world. The bovine estrous cycle, particularly the estrus, is tightly controlled by the complex interplay of various organs and hormones. Mathematical modeling of the bovine estrous cycle could help in understanding the dynamics of this complex biological system. The authors, therefore, developed a mechanistic mathematical model for the estrous cycle of Indian crossbred cows that exclusively includes the key hormones that interact to control these processes. The authors used the data generated by them for approximately 163 estrous cycles having 1793 readings related to different hormones like LH, FSH, progesterone, estrogen, etc., under the control of the hypothalamo-pituitary-gonadal (ovarian) axis. The developed model was capable enough to generate successive estrous cycles of 21-day length. The model contains seven differential equations and 34 parameters. By simulating the developed model, a set of equations and parameters that were obtained were found to describe vividly the system consistent with empirical knowledge. In conclusion, the authors for the first time developed a mechanistic mathematical model of the estrous cycle for the Indian crossbred cows and it can be used for predicting hormonal inter milieu of the complex biological system without spending much time and money towards laboratory estimation. The developed model may also be used as a basis for more elaborate models that can predict the effects of external manipulations and genetic differences in Indian bovines. Keywords: Indian crossbred cow, hormones, differential equations, systems biology Cite this Article Mondal M, Karunakaran M, Ghosh MK et al. Development of an Endocrine Profiles Based Mathematical Model for The Estrous Cycle in Indian Crossbred Cows. Research & Reviews: Journal of Veterinary Science and Technology . 2015; 4(3): 35–38p.
在集约化管理条件下饲养的高产奶牛的生育力下降不仅对印度而且对整个世界都是一个很大的问题。牛的发情周期，特别是发情，受到各种器官和激素复杂的相互作用的严格控制。牛发情周期的数学建模有助于理解这一复杂生物系统的动力学。因此，作者为印度杂交奶牛的发情周期建立了一个机械数学模型，该模型只包括相互作用控制这些过程的关键激素。在下丘脑-垂体-性腺(卵巢)轴的控制下，作者使用了他们产生的大约163个发情周期的数据，其中有1793个与LH, FSH，黄体酮，雌激素等不同激素相关的读数。所建立的模型足以产生21天的连续发情周期。该模型包含7个微分方程和34个参数。通过对所建立的模型进行模拟，发现所得到的一组方程和参数能够生动地描述系统，与经验知识相一致。总之，作者首次建立了印度杂交奶牛发情周期的机械数学模型，该模型可用于预测复杂生物系统的激素环境，而无需花费大量时间和金钱进行实验室估计。开发的模型还可以作为更复杂的模型的基础，以预测外部操作和印度牛的遗传差异的影响。关键词:印度杂交牛，激素，微分方程，系统生物学引用本文Mondal M, Karunakaran M, Ghosh MK等基于内分泌谱的印度杂交奶牛发情周期数学模型的建立研究与评述:兽医科学与技术杂志。2015;4 (3): 35-38p。
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Pub Date : 2018-04-21DOI: 10.4172/2157-7579.1000535
E. Mulatu, A. Feyisa
Lumpy skin disease, which is caused by lumpy skin disease virus, is among the major health problems affecting the livestock industry of most African countries. Skin lesions are the major sources of infection; although the virus is evacuated via different body secretions and excretions including semen. Thus, susceptible hosts contract the virus principally by mechanical means from hematophagous arthropods, including biting flies, mosquitoes and ticks. Transstadial and transovarial persistence in varies species of ticks is also possible. Following infection, characteristic lumpy skin disease lesions may explode from 7 to 14 days post infection under experimental conditions whereas in natural cases it takes 2 to 5 weeks. Lumpy skin disease is manifested by distinguishing firm, circumscribed, few (mild forms) to multiple (severe forms) skin nodules, which sometimes involve mucous membranes of respiratory system, urogenital system and other internal organs. Subsequently, milk production lessen, abortion, temporary or permanent sterility, damage to hide and deaths will occur which further contribute to a momentous economic loss in cattle producing countries. Therefore, large-scale vaccination combined with other appropriate control measures are the most effective way of limiting the spread and economic impact due to lumpy skin disease. This review is designed with the aim of providing, latest information on the biology of lumpy skin disease virus, mechanism of spread, clinical and pathological features of lumpy skin disease.
{"title":"Review: Lumpy Skin Disease","authors":"E. Mulatu, A. Feyisa","doi":"10.4172/2157-7579.1000535","DOIUrl":"https://doi.org/10.4172/2157-7579.1000535","url":null,"abstract":"Lumpy skin disease, which is caused by lumpy skin disease virus, is among the major health problems affecting the livestock industry of most African countries. Skin lesions are the major sources of infection; although the virus is evacuated via different body secretions and excretions including semen. Thus, susceptible hosts contract the virus principally by mechanical means from hematophagous arthropods, including biting flies, mosquitoes and ticks. Transstadial and transovarial persistence in varies species of ticks is also possible. Following infection, characteristic lumpy skin disease lesions may explode from 7 to 14 days post infection under experimental conditions whereas in natural cases it takes 2 to 5 weeks. Lumpy skin disease is manifested by distinguishing firm, circumscribed, few (mild forms) to multiple (severe forms) skin nodules, which sometimes involve mucous membranes of respiratory system, urogenital system and other internal organs. Subsequently, milk production lessen, abortion, temporary or permanent sterility, damage to hide and deaths will occur which further contribute to a momentous economic loss in cattle producing countries. Therefore, large-scale vaccination combined with other appropriate control measures are the most effective way of limiting the spread and economic impact due to lumpy skin disease. This review is designed with the aim of providing, latest information on the biology of lumpy skin disease virus, mechanism of spread, clinical and pathological features of lumpy skin disease.","PeriodicalId":36826,"journal":{"name":"Iranian Journal of Veterinary Science and Technology","volume":"138 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2018-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77456296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-21DOI: 10.37591/RRJOVST.V4I3.571
Sanjiv Kumar, R. Tiwary
he work was conducted on an adult German shepherd bitch having a growing lump in the mammary gland. Tentatively it appeared to be a case of benign tumour of first left thoracic mammary gland. Sample was collected for cytopathological and histopathological examination of the affected tissue. FNAC was done by Papanicoloau’s stain while routine paraffin embedding technique was employed for histopathological examination, which revealed it to be a case of mammary gland fibroadenoma which was then surgically removed successfully with full recovery. Keywords: Mammary gland fibroadenoma, benign tumour, FNAC Cite this Article Sanjiv Kumar, Ramesh Tiwary. Mammary Gland Fibroadenoma in a Bitch. Research & Reviews: Journal of Veterinary Science and Technology . 2015; 4(3): 27–28p.
{"title":"Mammary Gland Fibroadenoma in a Bitch","authors":"Sanjiv Kumar, R. Tiwary","doi":"10.37591/RRJOVST.V4I3.571","DOIUrl":"https://doi.org/10.37591/RRJOVST.V4I3.571","url":null,"abstract":"he work was conducted on an adult German shepherd bitch having a growing lump in the mammary gland. Tentatively it appeared to be a case of benign tumour of first left thoracic mammary gland. Sample was collected for cytopathological and histopathological examination of the affected tissue. FNAC was done by Papanicoloau’s stain while routine paraffin embedding technique was employed for histopathological examination, which revealed it to be a case of mammary gland fibroadenoma which was then surgically removed successfully with full recovery. Keywords: Mammary gland fibroadenoma, benign tumour, FNAC Cite this Article Sanjiv Kumar, Ramesh Tiwary. Mammary Gland Fibroadenoma in a Bitch. Research & Reviews: Journal of Veterinary Science and Technology . 2015; 4(3): 27–28p.","PeriodicalId":36826,"journal":{"name":"Iranian Journal of Veterinary Science and Technology","volume":"355 1","pages":"27-28"},"PeriodicalIF":0.0,"publicationDate":"2018-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76418006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-21DOI: 10.37591/RRJOVST.V4I3.569
P. Krishnamoorthy, M. Satyanarayana, B. Shome, Suguna Rao, H. Rahman
In this study, haemato-biochemical changes in mouse mastitis by inoculating Staphylococcus epidermidis, S. chromogenes, S. haemolyticus and S. Aureus isolated from bovine milk was studied. Blood was collected by intracardiac route at 6, 12, 24, 48, 72 and 96 h. Haematological changes noticed were: white blood cells (WBC) decreased in S. haemolyticus and S. chromogenes at 48 h; S. haemolyticus and S. aureus at 96 h. S. haemolyticus showed increased lymphocyte at 24 and 48 h and in S. aureus at 48 h. Neutrophil was decreased in S. haemolyticus and S. aureus group. Glucose increased at 6 h with three Coagulase Negative Staphylococcus (CNS) species and S. aureus. Glucose and total protein increased in S. chromogenes infected mice. Globulin levels showed decrease in S. epidermidis and S. aureus. Albumin globulin ratio showed decreasing trend in S. chromogenes and S. haemolyticus inoculated mice. Aspartate aminotransferase (AST) level increased at 6 and 12 h in all infected groups. Alanine aminotransferase increased at 12 and 48 h in S. chromogenes and S. aureus respectively. Lactate dehydrogenase (LDH) increased at 6 h in all the infected mice. Thus, three CNS species induced haemato-biochemical changes in haemoglobin, WBC, neutrophil, glucose, total protein, AST and LDH which may be used as indicators for diagnosis of CNS species mastitis in mice. Keywords: Staphylococcus species, mouse mastitis, haemato-biochemical changes Cite this Article Krishnamoorthy P, Satyanarayana ML, Shome BR, et al. Haemato-Biochemical Changes in Staphylococcal Species induced Mastitis in Mouse Model + . Research & Reviews: Journal of Veterinary Science and Technology . 2015; 4(3): 8–14p.
{"title":"Haemato-Biochemical Changes in Staphylococcal Species Induced Mastitis in Mouse Model","authors":"P. Krishnamoorthy, M. Satyanarayana, B. Shome, Suguna Rao, H. Rahman","doi":"10.37591/RRJOVST.V4I3.569","DOIUrl":"https://doi.org/10.37591/RRJOVST.V4I3.569","url":null,"abstract":"In this study, haemato-biochemical changes in mouse mastitis by inoculating Staphylococcus epidermidis, S. chromogenes, S. haemolyticus and S. Aureus isolated from bovine milk was studied. Blood was collected by intracardiac route at 6, 12, 24, 48, 72 and 96 h. Haematological changes noticed were: white blood cells (WBC) decreased in S. haemolyticus and S. chromogenes at 48 h; S. haemolyticus and S. aureus at 96 h. S. haemolyticus showed increased lymphocyte at 24 and 48 h and in S. aureus at 48 h. Neutrophil was decreased in S. haemolyticus and S. aureus group. Glucose increased at 6 h with three Coagulase Negative Staphylococcus (CNS) species and S. aureus. Glucose and total protein increased in S. chromogenes infected mice. Globulin levels showed decrease in S. epidermidis and S. aureus. Albumin globulin ratio showed decreasing trend in S. chromogenes and S. haemolyticus inoculated mice. Aspartate aminotransferase (AST) level increased at 6 and 12 h in all infected groups. Alanine aminotransferase increased at 12 and 48 h in S. chromogenes and S. aureus respectively. Lactate dehydrogenase (LDH) increased at 6 h in all the infected mice. Thus, three CNS species induced haemato-biochemical changes in haemoglobin, WBC, neutrophil, glucose, total protein, AST and LDH which may be used as indicators for diagnosis of CNS species mastitis in mice. Keywords: Staphylococcus species, mouse mastitis, haemato-biochemical changes Cite this Article Krishnamoorthy P, Satyanarayana ML, Shome BR, et al. Haemato-Biochemical Changes in Staphylococcal Species induced Mastitis in Mouse Model + . Research & Reviews: Journal of Veterinary Science and Technology . 2015; 4(3): 8–14p.","PeriodicalId":36826,"journal":{"name":"Iranian Journal of Veterinary Science and Technology","volume":"13 1","pages":"8-14"},"PeriodicalIF":0.0,"publicationDate":"2018-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82472469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-21DOI: 10.37591/RRJOVST.V4I2.546
Ajeet Kumar, Anil Gattani, Gurminder Singh, R. Tiwary, S. Samantaray
The study was conducted on fifteen dogs (n = 8 demodicosis, n = 7 healthy) brought to Veterinary Clinics, Bihar Veterinary College Clinics to determine Antioxidant Status and Lipid Peroxidation in erythrocyte of Demodex canis infested dog. Activity of catalase (CAT) and superoxide dismutase (SOD) and Thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) concentration were estimated in erythrocytes of dogs. Activity of CAT, SOD and TBARS concentration were higher in infected dogs than healthy dogs while and GSH concentration was higher in healthy dog than infected dogs. The results of the present study suggest that Demodex canis infestation cause oxidative stress in erythrocyte of dogs. Keywords: Demodex, SOD, GSH, Antioxidant, CAT Cite this Article Ajeet Kumar, Anil Gattani, Singh GD., et al. Antioxidant Status and Lipid Peroxidation in Erythrocyte of Dog Infested with Demodex canis. Research & Reviews: Journal of Veterinary Science and Technology (RRJoVST). 2015; 4(2): 1–3p.
{"title":"Antioxidant Status and Lipid Peroxidation in Erythrocyte of Dog Infested with Demodex canis","authors":"Ajeet Kumar, Anil Gattani, Gurminder Singh, R. Tiwary, S. Samantaray","doi":"10.37591/RRJOVST.V4I2.546","DOIUrl":"https://doi.org/10.37591/RRJOVST.V4I2.546","url":null,"abstract":"The study was conducted on fifteen dogs (n = 8 demodicosis, n = 7 healthy) brought to Veterinary Clinics, Bihar Veterinary College Clinics to determine Antioxidant Status and Lipid Peroxidation in erythrocyte of Demodex canis infested dog. Activity of catalase (CAT) and superoxide dismutase (SOD) and Thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) concentration were estimated in erythrocytes of dogs. Activity of CAT, SOD and TBARS concentration were higher in infected dogs than healthy dogs while and GSH concentration was higher in healthy dog than infected dogs. The results of the present study suggest that Demodex canis infestation cause oxidative stress in erythrocyte of dogs. Keywords: Demodex, SOD, GSH, Antioxidant, CAT Cite this Article Ajeet Kumar, Anil Gattani, Singh GD., et al. Antioxidant Status and Lipid Peroxidation in Erythrocyte of Dog Infested with Demodex canis. Research & Reviews: Journal of Veterinary Science and Technology (RRJoVST). 2015; 4(2): 1–3p.","PeriodicalId":36826,"journal":{"name":"Iranian Journal of Veterinary Science and Technology","volume":"6 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2018-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82526274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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