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Molecular detection of mouse hepatitis virus in laboratory mouse colonies. 小鼠肝炎病毒在实验室小鼠菌落中的分子检测。
Q4 Veterinary Pub Date : 2019-02-01 DOI: 10.22067/VETERINARY.V11I2.77631
R. Fallahi, F. Abedini, G. Shokri
The animal health monitoring is required to issue health certificates. The viral hepatitis virus is one of the most important infectious agents in mice breeding colonies. This research used RT-PCR to identify contaminations to mouse hepatitis virus. 18 out of 29 specimens were found to be infected, a prevalence of 62%. PCR product was purified and sequenced. Phylogenetic analysis revealed that the identified strain in this study was closely related to a strain reported from France. In the conventional system, contamination with different infectious agents is inevitable, thus it is better to replace the contaminated colonies with clean animals.
动物健康监测需要出具健康证明。病毒性肝炎病毒是小鼠繁殖群体中最重要的传染病之一。本研究采用RT-PCR方法鉴定小鼠肝炎病毒污染。29个标本中有18个被发现感染,患病率为62%。对PCR产物进行纯化和测序。系统发育分析表明,本研究鉴定的菌株与法国报道的一株菌株亲缘关系密切。在常规系统中,不同传染因子的污染是不可避免的,因此最好用干净的动物代替被污染的菌落。
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引用次数: 1
Severe subcutaneous, muscular and visceral coenurosis in a goat 山羊严重的皮下、肌肉和内脏神经症
Q4 Veterinary Pub Date : 2019-02-01 DOI: 10.22067/VETERINARY.V11I2.80397
S. Azizi, Mehdi Amirmohammadi, R. Kheirandish, S. R. Nourollahi-Fard
Coenurosis (gid or sturdy) is a zoonotic disease that is caused by Taenia multiceps metacestode. It is common in small ruminants. The cysts in sheep are more cerebral, while are noncerebral in goats. Coenurosis decreases production, and results in the death of the affected animals and in the disposal of the organs or even carcasses in severe infection. The present study describes severe subcutaneous coenuri associated with contamination in other tissues including the skeletal muscles and visceral organs. A remarkable clinical observation was the aggregation of cysts in variable sizes in the subcutaneous tissue of whole body. Subcutaneous tissue is not a common site for cyst formation. Coenurosis was confirmed based on the morphological characteristics of the cysts including the clusters of protoscolices and rostellar hooks.
犬尿症是一种人畜共患疾病,由多节带绦虫引起。它在小型反刍动物中很常见。绵羊的囊肿更多是大脑性的,而山羊的囊肿则是非大脑性的。犬尿症会降低产量,导致受影响动物死亡,并在严重感染时处理器官甚至尸体。本研究描述了与其他组织(包括骨骼肌和内脏器官)污染相关的严重皮下犬尿症。一个显著的临床观察是全身皮下组织中大小不等的囊肿聚集。皮下组织不是囊肿形成的常见部位。根据囊肿的形态学特征,包括原头节和花钩簇,证实了犬尿症。
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引用次数: 1
Study on Challenges and Opportunities of Beekeeping in Gimbi District,West Wollega Zone, Ethiopia 埃塞俄比亚西沃勒加区Gimbi地区养蜂业的挑战与机遇研究
Q4 Veterinary Pub Date : 2018-11-28 DOI: 10.4172/2157-7579.1000569
Amanuel A. Bekuma, T. Zeryehun
The study was carried out in Gimbi district, west Wollega zone, Ethiopia to assess beekeeping constraints and exploit the existing opportunities for beekeeping. The study used interview, survey and field observation as method of looking for answers for research questions. Purposive and simple random sampling techniques were used to select study sites and household respondents. 156 total household respondents were interviewed using semistructured questionnaires during the study period and SPSS software version 23 was used to analyze the gathered data. 5.42 ± 0.18, 10.23 ± 0.36 and 15.69 ± 0.59 kg were the average honey yield annually from traditional, intermediate and improved hive, respectively. Quality of honey (60.9%), distance from the market place (18.59%) and consumer’s preference (12.82%) were among the most factors governing the price of honey. Even though availability of bee forage and water, presence of immense number of bee density, high demand for local honey from honey traders and consumers and government attention to this sub-sector were encountered as opportunities for beekeeping development, improper application of agro-chemical inputs (1st), high cost of modern hive and equipment (2nd), diseases (3rd) and pests and predators (4th) were the challenges. Characterization of honeybees of the area, diseases and pest and predators needs further study.
该研究在埃塞俄比亚西沃勒加区Gimbi区进行,以评估养蜂业的制约因素并利用现有的养蜂业机会。本研究采用访谈、调查、实地观察等方法寻找研究问题的答案。采用有目的和简单的随机抽样技术选择研究地点和家庭受访者。在研究期间,采用半结构式问卷对156户家庭受访者进行了访谈,并使用SPSS 23版软件对收集到的数据进行分析。传统蜂箱、中间蜂箱和改良蜂箱的年平均产蜜量分别为5.42±0.18、10.23±0.36和15.69±0.59 kg。蜂蜜的质量(60.9%)、与市场的距离(18.59%)和消费者的偏好(12.82%)是影响蜂蜜价格的主要因素。尽管蜜蜂饲料和水的可用性、蜜蜂密度的巨大、蜂蜜贸易商和消费者对当地蜂蜜的高需求以及政府对这一细分行业的关注都是养蜂业发展的机遇,但农用化学品投入的不当使用(第一)、现代蜂箱和设备的高成本(第二)、疾病(第三)、害虫和捕食者(第四)是挑战。该地区的蜜蜂特征、病虫害和捕食者需要进一步研究。
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引用次数: 3
Preparation and in vitro evaluation of chitosan-based films for the sustained delivery of enrofloxacin 壳聚糖基恩诺沙星持续给药膜的制备及体外评价
Q4 Veterinary Pub Date : 2018-10-01 DOI: 10.22067/VETERINARY.V10I1.70288
A. Rassouli, S. K. Falahatipour, Y. H. Ardakani, H. A. Javar, Katayoun Kiani, T. Z. Salehi
The implantable drug products are developed mainly to sustain the drug release. This study was conducted to formulate and evaluate cross-linked films of chitosan/β-glycerophosphate (β-GP) for the sustained delivery of enrofloxacin (ENR). Two types of formulations, single-layer (F1 and F2) and triple-layer (F3 and F4) films, were prepared. In vitro drug release, kinetic modelling, Fourier transform infrared spectroscopy (FTIR) spectra, morphological and microbiological studies were performed. Drug release from F1 and F2 continued up to 5 hours but from F3 and F4, it was extended over 96 and 168 hours, respectively. The cumulative drug release for F1, F2, F3 and F4 were 72.6, 70.1, 90.5 and 82.4%, respectively. The inhibition zones of bacterial growth by using positive controls and single layer films were significantly greater than those of triple-layer films (p < 0.05), indicating sustained drug release pattern of the multi-layer films. These findings suggest that the triple-layer chitosan/β-GP films could be effective to deliver ENR for a long period.
植入式药物产品的开发主要是为了维持药物的释放。本研究旨在制备和评价壳聚糖/β-甘油磷酸(β-GP)交联膜用于恩诺沙星(ENR)的持续递送。制备了两种类型的制剂,单层(F1和F2)和三层(F3和F4)膜。进行了体外药物释放、动力学建模、傅立叶变换红外光谱、形态学和微生物学研究。F1和F2的药物释放持续达5小时,但F3和F4的药物释放分别延长了96和168小时。F1、F2、F3和F4的累积药物释放率分别为72.6%、70.1%、90.5%和82.4%。使用阳性对照和单层膜对细菌生长的抑制区显著大于三层膜(p<0.05),表明多层膜具有持续的药物释放模式。这些发现表明,三层壳聚糖/β-GP膜可以长期有效地递送ENR。
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引用次数: 0
Effect of Zataria multiflora essential oil on rooster semen during storage at 4°C 多花Zataria精油对4°C保存公鸡精液的影响
Q4 Veterinary Pub Date : 2018-09-26 DOI: 10.22067/veterinary.v10i1.72126
Fahimeh Geraminezhad, M. R. Mehr
Experiment was conducted to determine effect of Zataria multiflora boiss essential oil on stored spermatozoa. Semen collection was performed by using 15 mature roosters twice a week at four times. In each session, ejaculates were pooled and split into seven parts. The amounts of 0 (EO0), 50 (EO50), 100 (EO100), 200 (EO200), 400 (EO400), 600 (EO600) and 1000 (EO1000) ng/ml Zataria multiflora boiss essential oil were added to each part. Samples were chilled to 4°C and maintained for 72 h. Sperm assessment was performed at 0, 24, 48 and 72 h. Lipid peroxidation was evaluated after 48 h. Results showed that there was no interaction between Zataria multiflora essential oil and incubation time on membrane integrity, sperm motility and viability (p > 0.05). The highest sperm progressive motility (80.43%), viability (86.31%) and functional membrane integrity (85.81%) was observed in EO200 (p < 0.05). The lowest sperm motility (61.31%) and viability (73.31%) was observed in EO1000 (p < 0.05). The concentrations of malondialdehyde was lowest in EO200 (0.17 nM/ml, p < 0.05). Therefore, addition of 200 ng/ml Zataria multiflora boiss essential oil to semen improved longevity of rooster spermatozoa at 4°C.
通过实验研究何首乌挥发油对储存精子的影响。采集15只成熟公鸡的精液,每周2次,分4次采集。在每次实验中,射精被分成七部分。各部位分别加入0 (EO0)、50 (EO50)、100 (EO100)、200 (EO200)、400 (EO400)、600 (EO600)、1000 (EO1000) ng/ml的复方扎连精油。冷冻至4℃,保存72 h,分别在0、24、48和72 h进行精子评价,48 h后进行脂质过氧化评价。结果表明,多花扎扎草精油与孵育时间对膜完整性、精子活力和活力无交互作用(p < 0.05)。EO200组精子进动率(80.43%)、活力(86.31%)和功能膜完整性(85.81%)最高(p < 0.05)。EO1000组精子活力最低(61.31%),活力最低(73.31%)(p < 0.05)。EO200中丙二醛浓度最低(0.17 nM/ml, p < 0.05)。因此,在4℃条件下,在精液中添加200 ng/ml多花扎莉精油可以提高公鸡精子的寿命。
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引用次数: 2
In vitro evaluation of acaricidal activity of aqueous ozone against Dermanyssus gallina 水臭氧对鸡皮螨的体外杀螨活性评价
Q4 Veterinary Pub Date : 2018-09-26 DOI: 10.22067/veterinary.v10i1.70015
Hamid Dehghan Banadakei, A. Moshaverinia, M. Khodaparast, G. Kalidari
The aim of this study was to investigate the in vitro acaricidal effect of aqueous ozone against Dermanyssus gallinae (Acari: Dermanyssidae) under laboratory conditions. For this purpose, aqueous ozone at concentrations of 1, 2, 3, 4, 5 and 10 ppm were prepared, and five replicate experiments were carried out using 400 µl of each concentration sprayed on every treatment batch of mites (plus a distilled water control). The mortality rate of mites in treatment and control groups were assessed 24 h post exposure. The mortality rate obtained by concentrations of 4, 5, and 10 ppm were significantly different from the control group (p < 0.05). The highest mortality rate (63.99%) was observed at concentration of 10 ppm. In this study, aqueous ozone showed a dose-dependent acaricidal potency against D. gallinae.
本研究的目的是在实验室条件下,研究水臭氧对鸡皮螨(革螨科)的体外杀螨效果。为此,制备了浓度为1、2、3、4、5和10 ppm的水溶液臭氧,并在每个处理批次的螨虫上喷洒每种浓度的400µl(加上蒸馏水对照),进行了5次重复实验。观察处理组和对照组接触24 h后螨类的死亡率。4、5和10 ppm浓度组的死亡率与对照组有显著差异(p < 0.05)。浓度为10 ppm时死亡率最高,为63.99%。在本研究中,水溶液臭氧显示出剂量依赖性的杀螨力。
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引用次数: 0
The hematological profile changes in Saanen goat kids from birth to 3 months of age 从出生到3个月大的萨南山羊的血液学特征的变化
Q4 Veterinary Pub Date : 2018-09-26 DOI: 10.22067/veterinary.v10i1.66272
S. abdolvahabi, M. Zaeemi, M. Mohri, A. Naserian
This study performed to determine hematological parameters in Saanen goats’ kids from birth until 3 months of age. The whole blood specimens were collected from 20 clinically healthy goat kids (15 females and 5 males). The blood specimens were obtained at 24-48h after birth and 10 ± 2, 28 ± 2, 56 ± 2 and 84 ± 2 days. Hematological variables were determined based on reference laboratory methods. Results showed significant age related changes for most factors (p < 0.05) except for MCHC and the number of monocytes, eosinophils, and band neutrophils. There were significant differences with first sampling time for all parameters (p < 0.01) except for the number of monocytes, eosinophils and band neutrophils. The results of the present study ould be used as reference values for proper interpretation of laboratory results.
本研究测定了萨宁山羊从出生到3个月的血液学参数。采集临床健康山羊幼崽20只(母山羊15只,公山羊5只)全血标本。分别于出生后24 ~ 48h、10±2、28±2、56±2、84±2 d采血。根据参考实验室方法测定血液学变量。结果显示,除MCHC、单核细胞、嗜酸性粒细胞和带状中性粒细胞数量外,其他因素均与年龄相关(p < 0.05)。除单核细胞、嗜酸性粒细胞和带状中性粒细胞数量外,其他参数与首次采样时间差异均有统计学意义(p < 0.01)。本研究结果可作为正确解释实验室结果的参考值。
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引用次数: 4
Occurrence, hematologic and serum biochemical characteristics of neonatal isoerythrolysis in Arabian horses of Iran 伊朗阿拉伯马新生儿异红细胞溶解的发生、血液学和血清生化特征
Q4 Veterinary Pub Date : 2018-09-26 DOI: 10.22067/veterinary.v10i1.71821
S. Jalali, M. Razi-Jalali, A. Ghadrdan-Mashhadi, Maryam Motamed-Zargar
Neonatal isoerythrolysis is a major cause of anemia in newborn foals. However, there are no documented data regarding the occurrence of neonatal isoerythrolysis in Arabian horses of Iran, which are mostly raised in Khouzestan province. Hence, this study was carried out to investigate the occurrence of neonatal isoerythrolysis in Arabian horses of Khouzestan and assess the hematologic and serum biochemical profile of affected foals. A total of 20 neonatal foals, under one week of age, and their dams were involved in this study. Clinical examinations revealed no abnormality except in one foal with icteric mucous membranes, lethargy, tachycardia, tachypnea, hypothermia and hemoglobinuria which led to death. The diagnosis of neonatal isoerythrolysis was made based on erythrocyte agglutination in cross-match test between the mare serum and foal erythrocytes with the titer of 1:128, while other studied cases were assumed negative according to the test results. In the laboratory assessment, the foal with hemolytic anemia showed a major decline in hematocrit, hemoglobin concentration and erythrocyte count along with considerable leukocytosis and neutrophilia. Serum total and direct bilirubin concentrations in the NI case was about ten times higher than in the rest of the foals. This study revealed that neonatal isoerythrolysis can occur in Arabian foals of Khouzestan and is associated with severe anemia and icterus which may lead to death. These findings can be beneficial in the establishment of preventive measures in Arabian horse breeding industry in the region, as well as improving therapeutic methods.
新生儿等红细胞溶解是新生儿小马驹贫血的主要原因。然而,没有关于伊朗阿拉伯马新生儿等红细胞裂解发生的记录数据,这些马大多在Khouzestan省饲养。因此,本研究旨在调查Khouzestan阿拉伯马新生儿等红细胞溶解的发生情况,并评估受影响小马驹的血液学和血清生化特征。共有20只一周以下的新生小马驹及其母马参与了这项研究。临床检查显示,除了一匹小马驹出现黄疸粘膜、嗜睡、心动过速、呼吸急促、体温过低和血红蛋白尿导致死亡外,没有任何异常。新生儿等红细胞溶解的诊断是基于母马血清和小马驹红细胞滴度为1:128的交叉配型试验中的红细胞凝集,而其他研究病例根据试验结果假定为阴性。在实验室评估中,患有溶血性贫血的小马驹表现出红细胞比容、血红蛋白浓度和红细胞计数的显著下降,同时伴有大量白细胞增多和中性粒细胞增多。NI病例的血清总胆红素和直接胆红素浓度大约是其他小马驹的十倍。这项研究表明,Khouzestan的阿拉伯小马驹可能发生新生儿等红细胞溶解,并与可能导致死亡的严重贫血和黄疸有关。这些发现有助于该地区阿拉伯马养殖业制定预防措施,以及改进治疗方法。
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引用次数: 0
Molecular epidemiology of Campylobacter Fetus in aborted fetuses of Baluchi sheep in Sistan region 锡斯坦地区俾路支羊流产胎儿弯曲杆菌的分子流行病学研究
Q4 Veterinary Pub Date : 2018-09-26 DOI: 10.22067/veterinary.v10i1.69012
Ebrahim Hossein Abadi, D. Saadati, M. Najimi, M. Hassanpour
Campylobacter is one of the main bacterial causes of ewe abortion throughout the world. Campylobacter infections are now considered as zoonoses. The objective of this study was an investigation of Campylobacter fetus prevalence among aborted ovine fetuses in the Sistan region (north of Sistan and Baluchestan province). In the present study, spleen and abomasum content samples were obtained from 78 aborted lambs of Baluchi sheep. The samples were examined for campylobacter contamination using PCR method. The overall prevalence of campylobacter infection was 7.7%. The prevalence of infection in fetuses aged three months and under were significantly higher than that in fetuses older than three months. The result of this study showed that C. fetus should be considered as one of the infectious causes of abortion among sheep flocks in Sistan region.
弯曲杆菌是导致世界各地母羊流产的主要细菌之一。弯曲杆菌感染现在被认为是人畜共患疾病。本研究的目的是调查锡斯坦地区(锡斯坦和俾路支省北部)流产绵羊胎儿中弯曲杆菌的胎儿患病率。在本研究中,从78只巴鲁奇绵羊流产的羔羊身上采集了脾脏和皱胃含量的样本。使用聚合酶链式反应方法对样品进行弯曲杆菌污染检测。弯曲杆菌感染的总患病率为7.7%。三个月及以下胎儿的感染率显著高于三个月以上胎儿。本研究结果表明,C.胎儿应被认为是泗滩地区绵羊群流产的传染原因之一。
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引用次数: 2
Cardiotoxicity of Plants in Iran: a Review 伊朗植物的心脏毒性研究进展
Q4 Veterinary Pub Date : 2018-09-26 DOI: 10.22067/VETERINARY.V10I1.72262
M. Aslani
In this review an attempt has been made to review the cardiotoxic effects of poisonous plants which are found in Iran. Among various plant species growing in different regions of Iran, a large number of plants contain chemical compounds which are toxic to animals and humans. Among those poisonous plants, cardiotoxic plants are important due to the acute nature of their toxicity and frequent lethal livestock and human intoxications. Cardiotoxic plants of Apocynaceae family, Nerium oleander, N. indicum and Thevetia peruviana contain cardiac glycosides including oleandrin, oleandroside and thevetin A-C. Plants of Taxaceae family, Taxus baccata and T. brevifolia contain taxine alkaloids, including taxine A and B. The toxic effects of cardiac glycosides are primarily attributed to inhibition of plasmalemmal Na+/ K+-ATPase which results into the accumulation of intracellular Ca2+ and, depending to its severity, inotropic or arrhythmic effects are seen. Taxine B, the prominent alkaloid in Taxus spp. block sodium and calcium channels preferentially in cardiac myocytes, thus causing conduction abnormalities. Various cardiac arrhythmias in acute cases of poisoning with aforementioned plants result in acute heart failure and death. Post mortem findings are non-diagnostic and toxicological analysis of gastrointestinal tract content or body fluids is used for detection of cardiac glycosides and taxines.
在这篇综述中,试图回顾在伊朗发现的有毒植物的心脏毒性作用。在伊朗不同地区生长的各种植物中,大量植物含有对动物和人类有毒的化合物。在这些有毒植物中,心毒性植物是重要的,因为它们的毒性是急性的,并且经常致命的牲畜和人类中毒。Cardiotoxic夹竹桃科植物的家庭,夹竹桃夹竹桃:indicum和Thevetia peruviana包含心脏苷包括oleandrin oleandroside和黄花夹竹桃苷a - c。红豆杉科植物,烟叶红豆杉和短叶红豆杉含有豆杉碱生物碱,包括豆杉A和豆杉b。心脏糖苷的毒性作用主要归因于抑制细胞质Na+/ K+- atp酶,导致细胞内Ca2+的积累,并且根据其严重程度,可以看到肌力或心律失常的作用。Taxine B是红豆杉中重要的生物碱,它优先阻断心肌细胞中的钠通道和钙通道,从而导致传导异常。上述植物急性中毒后,各种心律失常可导致急性心力衰竭和死亡。尸检结果不能用于诊断,胃肠内容物或体液的毒理学分析用于检测心脏糖苷和的士。
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引用次数: 1
期刊
Iranian Journal of Veterinary Science and Technology
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