In recent years, molecular techniques for the aetiological diagnosis of infective endocarditis (IE) have attained relevance; however, they are not implemented in all laboratories. Our objective is to evaluate the usefulness of broad-spectrum polymerase chain reaction (PCR) technique followed by sequencing (universal PCR) on valve tissue as part of the IE diagnostic practice and to compare the results with classical methods.
Universal PCR showed a higher sensitivity than valve culture (80.8 vs 27.1%, P < .001) and allowed the identification of the causative agent or the confirmation of the aetiological diagnosis in case of discordance in 7.7% of patients. Regarding conventional diagnosis, blood culture stands out as the reference technique and Gram staining due to its high positive predictive value. The highest sensitivity and specificity was obtained considering classical and molecular microbiology together. The anatomopathological study presented a sensitivity of 83.9% and made it possible to assess the results of the microbiological diagnosis when there were discrepancies.
Universal PCR proved to be a valuable technique that completes the classical diagnosis. In an entity as complex as IE, it is necessary to use all the diagnostic tools at our disposal. All areas of laboratory diagnosis are complementary and not exclusive and should be assessed together.
This study aims to describe relationship between physical performance (PP) and health-related quality of life in patients with heart failure (HF).
This study used a cross-sectional design for data collection. Minnesota Living with Heart Failure Questionnaire (MLHFQ) was used as a measurement tool, while the Five Times Sit-to-Stand Test (FTSST) was used to assess PP. The data were processed by Pearson correlation coefficient, t-test, one-way ANOVAs, and Hierarchical multiple regression.
A total of 180 patients with HF participated in this study, with the mean age of respondents being 59.98 (11.86) years old. Among these respondents, 60% were male, with a mean PP of 9.56 (6.94) s and a mean MLHFQ of 43.14 (20.74). The results showed that MLHFQ had a significant correlation with HF medication (r = .16, P < .05), health status (r = .24, P < .01), FTSST (r = .40, P < .01), and MLHFQ was significantly associated with New York Heart Association (NYHA) Classification (F = 8.358, P < .001). There were three variables identified as predictors of MLHFQ, namely health status (β = −2.22), NYHA Class III (β = 1.27), and FTSST (β = 3.03), and were predicted to account for 31.1% of the variance in MLHFQ.
Efforts to increase PP from patients with HF can be an asset to improve health-related quality of life. Furthermore, health status and NYHA classifications are factors that can significantly affect health-related quality of life of patients with HF.
The care of patients with cardiovascular disease by specialist in cardiology has shown better results in different spectrums. However, the presence of an on-call cardiologist is still rare in non-tertiary hospitals.
STEMI was recorded consecutively between 2006 and 2016 in a second level center. We analyzed and compared two periods: regional heart attack care network without a cardiology guard (2006-2011) and a second period (2012-2016) with both measures working.
1524 patients were included, 47.2% after the start of cardiology shift. We observed an increase in primary angioplasty (34.3% to 75.7%, P < .001) as well as a reduction in door-to-balloon time of 64 minutes (220 [IQR, 167-290] to 156 [IQR, 130-197], P < .001). This translates into a shorter hospital stay as well as a reduction in complications during hospitalization, mainly recurrent ischemia. Although there is a trend towards lower hospital mortality, mortality during follow-up did not change with the cardiology guard.
The presence of a cardiologist on call increases the number of patients reperfused, and shortens the time until it. This translates into a shorter hospital stay, as well as lower short-term mortality. Our results indicate that the creation of the cardiological guard brings additional benefit to the care network for patients with STEMI.
ClinicalTrials.gov identifier: NCT02501070.