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ON THE EXTREMUM-SEEKING CONTROL DESIGN AND APPLICATION FOR ANAEROBIC DIGESTION PROCESSES 厌氧消化过程极值寻优控制设计与应用
Pub Date : 2019-09-30 DOI: 10.32006/eeep.2019.2.2328
Maobo Hu, Haoping Wang, Yang Tian, N. Christov, I. Simeonov
The paper deals with the optimization of nonlinear systems by using Extremum Seeking Control (ESC) without any prior knowledge of the system model. An Extend Kalman Filter based Extremum Seeking Control (EKF based ESC) is proposed, which can make the amplitude of perturbation signal variable and ensure convergence to zero, i.e. without steady-state oscillation. The proposed ESC algorithm makes also possible to obtain more accurate gradient estimate and more rapid ESC convergence. The proposed EKF based ESC algorithm is applied to a fifth-order model of anaerobic digestion process and its performances are compared with the performances of the classical ESC algorithm. Key Words: ESC, EKF, steady state oscillation, anaerobic digestion processes
本文研究了在不知道系统模型先验知识的情况下,利用极值寻求控制(ESC)对非线性系统进行优化。提出了一种基于扩展卡尔曼滤波的极值寻求控制(EKF based ESC),该控制可以使扰动信号的幅值可变,并保证收敛到零,即无稳态振荡。提出的ESC算法还可以获得更精确的梯度估计和更快的ESC收敛速度。将提出的基于EKF的ESC算法应用于厌氧消化过程的五阶模型,并与经典ESC算法的性能进行了比较。关键词:ESC, EKF,稳态振荡,厌氧消化过程
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引用次数: 4
GERMINATION OF TANACETUM CINERARIIFOLIUM SEEDS ON DIFFERENT SOIL TYPES 不同土壤类型柽桐种子萌发的研究
Pub Date : 2019-09-30 DOI: 10.32006/eeep.2019.2.6774
Vladimir Ilinkin
Tanacetum cinerariifolium (Trevir.) Sch. Bip. is a species of great economic importance because it contains Pyrethrins – natural insecticides with the potential to be the ideal means for pest control. Soil samples from different soil types (Skeletic Fluvisols, Rendzic Leptosol, Luvic Chernozem, Chromic Luvisols, Dystric-Eutric Cambisol, Pellic Vertisols) have been studied in order to determine the physicochemical soil indicators and the regressions between the soil characteristics and seed germination. Four hundred seeds (4х100) were planted in each soil type (in pots) under controlled conditions. Reliable statistically significant regressions, both negative (bulk density, sand fraction, available phosphorus) and positive (total porosity, silt and clay fractions, pH(H2O), CaCO3, Total Kjeldahl Nitrogen, Soil Organic Mater) with regard to seed germination have been established. The seeds planted in Rendzic Leptosol have shown the highest germination percentage, and the ones planted in Skeletic Fluvisols have shown the lowest germination percentage.Key words: Pyrethrum cinerariifolium Trev., Chrysanthemum cinerariifolium Bocc, seed germination, soil.
银杏(Tanacetum cinerariifolium)毕普原理图。是一种具有重要经济价值的物种,因为它含有拟除虫菊酯——一种天然杀虫剂,有可能成为控制害虫的理想手段。为了确定土壤理化指标和土壤特征与种子萌发的回归关系,研究了不同土壤类型(骨溶型、蓝壤型、露钙土型、铬溶型、难溶型、壤壤型)的土壤样品。在受控条件下,在每种土壤类型(盆栽)中种植400颗种子(4х100)。建立了与种子萌发相关的负回归(容重、沙粒分数、有效磷)和正回归(总孔隙度、粉土和粘土分数、pH(H2O)、CaCO3、总凯氏定氮、土壤有机质)。在Rendzic Leptosol中播种的种子萌发率最高,在skeleton Fluvisols中播种的种子萌发率最低。关键词:除虫菊;菊花,种子萌发,土壤。
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引用次数: 2
INFLUENCE OF MICRO-ENVIRONMENT ON YEAST POPULATION DYNAMICS 微环境对酵母种群动态的影响
Pub Date : 2019-09-15 DOI: 10.32006/eeep.2019.2.0513
J. Trosset, Sami Tliba, A. E. Ati, H. Friha, E. Mogensen, Jawher Kahouli, Robin V. Lacombe, Ouerdia Arkoun, S. Diop, R. Ledesma-Amaro, I. Boussaada
Abundance or scarcity of external nutrients is a metabolic trigger, especially for highly proliferative cells such as bacteria, yeasts, parasites or tumors. In presence of oxygen cells usually adopt efficient metabolism in order to maximize energy production yield in poor diet. If nutrient resource increases, a metabolic shift from efficient metabolism (respiration) to inefficient metabolism (fermentation) is reflecting a minimal cost principle of living systems to optimize fitness. This is known as the Crabtree/Warburg effect. Identifying a model that describes the population dynamics of cells and the input growth condition are the goals of this study. Proof of principle has been constructed using a battery of growth experiments on Crabtree-positive yeasts–Saccharomyces under various conditions of glucose in aerobic and micro-aerobic conditions. General cell growth model estimating metabolic shift has been constructed based on an Auto Regressive approach.Keywords: Yeast, Population dynamics, Modeling, Identification
外部营养物质的丰富或缺乏是代谢的触发因素,特别是对于细菌、酵母、寄生虫或肿瘤等高度增殖的细胞。在有氧气存在的情况下,细胞通常采用高效代谢,以便在不良饮食条件下最大限度地提高能量产量。如果营养资源增加,代谢从高效代谢(呼吸)到低效代谢(发酵)的转变反映了生命系统优化适应度的最小成本原则。这就是众所周知的克拉布特里/沃伯格效应。确定一个描述细胞种群动态和输入生长条件的模型是本研究的目标。在好氧和微氧条件下,对crabtree阳性酵母-酵母菌进行了一系列生长实验,建立了原理证明。基于自回归方法建立了估计代谢移位的一般细胞生长模型。关键词:酵母,种群动态,建模,鉴定
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引用次数: 0
CURRENT SITUATION AND SUSTAINABLE DEVELOPMENT OF WATER RESOURCES IN SENEGAL 塞内加尔水资源现状与可持续发展
Pub Date : 2019-03-31 DOI: 10.32006/EEEP.2019.1.0516
C. Faye, E. Gomis, S. Dièye
Water is essential for human life, the socio-economic improvement of the state and the protection of its natural environment. Senegal has a rich and diversified hydrological potential, most of whose surface water reserves are located in the basins of the Senegal and Gambia rivers and in groundwater. Unfortunately, water resources can be threatened by anthropogenic actions of various origins and by the adverse effects of climate change. This article aims to analyze the current state of water resources, water problems and prospects for the sustainable development of water resources in Senegal. Information is collected from secondary sources and available statistics (books and the Internet). The results show the importance of Senegal's water resources potential (in terms of surface and groundwater), water resources that are often severely deteriorated due to pollution, agricultural activities and the rised water demand from the population. This degradation is likely to worsen with population growth, development and climate change. For example, the Senegalese government has been conducting a water control policy for several decades aimed at providing the various sectors with water in sufficient quantity and of appropriate quality according to custom to accelerate development balanced. There are several policies and actions for the formulation of rules and regulations on the general use of water. To be in line with the sustainable development goals (SDG), including SDG 6, Senegal is committed to the sustainable management of water resources to ensure universal and equitable access to safe and affordable drinking water for all population by 2030.
水对人类生活、改善国家的社会经济和保护自然环境至关重要。塞内加尔具有丰富多样的水文潜力,其大部分地表水储量位于塞内加尔和冈比亚河流域和地下水中。不幸的是,水资源可能受到各种原因的人为活动和气候变化的不利影响的威胁。本文旨在分析塞内加尔的水资源现状、水资源问题以及水资源可持续发展的前景。信息收集自二手来源和可用的统计数据(书籍和互联网)。结果显示了塞内加尔水资源潜力的重要性(就地表水和地下水而言),由于污染、农业活动和人口对水的需求增加,水资源往往严重恶化。这种退化可能会随着人口增长、发展和气候变化而恶化。例如,塞内加尔政府几十年来一直在执行一项水控制政策,目的是根据习惯向各部门提供足够数量和适当质量的水,以加速平衡发展。有几项政策和行动用于制订关于一般用水的规则和条例。为实现包括可持续发展目标6在内的可持续发展目标,塞内加尔致力于可持续地管理水资源,确保到2030年所有人口普遍和公平地获得安全和负担得起的饮用水。
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引用次数: 2
BIODEGRADATION OF CRUDE OIL AND LIGNIN-MODIFYING ENZYME ACTIVITY OF WHITE ROT BASIDIOMYCETES 原油生物降解及白腐担子菌木质素修饰酶活性研究
Pub Date : 2019-03-31 DOI: 10.32006/EEEP.2019.1.2636
Tina Jokharidze, E. Kachlishvili, V. Elisashvili
In the present study, the ability of eighteen white-rot basidiomycetes was evaluated for crude oil biodegradation. Cerrena unicolor strains, Panus tigrinus 433, P. lecometei 903, Pleurotus ostreatus 70, Trametes maxima 403, and T. versicolor 159 showed especially abundant mycelial growth on the surface of agar covered with droplets of crude oil. In the submerged cultivation in the glucose (3 g/L) containing medium, complete decolorization of indicator Resazurin was observed during two weeks in the presence of Bjerkandera adusta 139, C. unicolor 303, Coriolopsis gallica 142, P. ostreatus 70, P. pulmonarius 148, and T. versicolor 159. When artificially oil-polluted soil was inoculated with fungal mycelium pre-grown on a mixture of wheat straw and mandarin peels the maximum degradation rate (65%) was obtained when C. unicolor 305 was incubated in the 1% oil-containing soil for 28 days. At the same cultivation conditions, P. ostreatus 2175 eliminated 43.9% of initial oil when its concentration in the soil was increased to 2%. In the lignocellulose-containing soil, neither glucose nor yeast extract enhanced oil degradation, but wetting of soil with the distilled water to maintain its humidity favored oil elimination. The tested WRB secreted lignin-modifying enzymes in the presence of petroleum hydrocarbons; the higher was the concentration of lignocellulosic substrate in the soil the higher was the fungi enzyme activity. However, the data received did not show any direct relationship between the fungi enzyme activity and the degree of oil elimination.Key words: mycoremediation, crude oil, basidiomycetes, cultivation conditions, lignin-modifying enzymes
研究了18种白腐担子菌对原油的生物降解能力。单色Cerrena、Panus tigrinus 433、P. lecometei 903、Pleurotus ostreatus 70、Trametes maxima 403和T. versicolor 159在被原油液滴覆盖的琼脂表面上菌丝生长特别丰富。在含葡萄糖(3 g/L)的培养基中,两周内观察到指示物Resazurin在Bjerkandera adusta 139、C. unicolor 303、Coriolopsis gallica 142、P. ostreatus 70、P. pulmonarius 148和T. versicolor 159的存在下完全脱色。用麦秸与柑桔皮混合培养的真菌菌丝体接种人工油污染土壤,在1%含油土壤中培养28 d,单色C. 305降解率最高(65%)。在相同的栽培条件下,当其在土壤中的浓度增加到2%时,P. ostreatus 2175消除了43.9%的初始油脂。在含木质纤维素的土壤中,葡萄糖和酵母提取物都不能促进油脂的降解,但用蒸馏水湿润土壤以保持其湿度有利于油脂的去除。在石油烃存在下,所测WRB分泌木质素修饰酶;土壤中木质纤维素底物浓度越高,真菌酶活性越高。然而,收到的数据并没有显示真菌酶活性与油脂消除程度之间的任何直接关系。关键词:真菌修复,原油,担子菌,培养条件,木质素修饰酶
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引用次数: 0
CLASSICAL AND NEW ASPECTS IN DEGRADATION OF AROMATIC XENOBIOTICS 芳香异种生物降解的经典与新进展
Pub Date : 2019-03-31 DOI: 10.32006/EEEP.2019.1.3753
K. E. Vasileva, I Tsvetomila Parvanova-Mancheva, N Venko Beschkov
Organic chemical mixtures are prevalent in waste waters from industrial and municipal sources as well as in contaminated groundwater. Phenols are pollutants found in wastewaters from oil refineries, chemical plants, explosives, resins and coke manufacture, coal conversion, pesticide and textile industries. The main contaminants of refinery wastewater include phenols, polycyclic aromatic hydrocarbons (PAHs) as well as heavy metals. Among these toxic pollutants, phenols are considered to be the most hazardous ones, and they are certainly the most difficult to remove. Phenolic compounds are toxic at relatively low concentration. Because of these low concentrations the most suitable methods for their removal are the microbial ones. The present work is a review of biodegradation of phenol. Degradation of phenol occurs as a result of the activity of a large number of microorganisms including bacteria, fungi and actinomycetes. There are reports on ma33ny microorganisms capable of degrading phenol through the action of variety of enzymes. These enzymes may include oxygenases, hydroxylases, peroxidases, tyrosinases, oxidases etc. Batch experiments were carried out in a different bioreactors. Biodegradation of organic chemicals by microbes using pure cultures can produce toxic intermediates. This problem may be overcome by the use of mixed cultures that have a wider spectrum of metabolite degradation properties. In this revew we described also some new technology for biodegradation of phenol like: different immobilization, FISH (Fluorescence in-situ hybridization) analysis, nanobiotechnologies and etc.Key words: phenol, biodegradation, microorganisms, enzymes, immobilization, FISH analysis, nanobiotechnologies
有机化学混合物普遍存在于工业和市政废水以及受污染的地下水中。苯酚是在炼油厂、化工厂、炸药、树脂和焦炭制造、煤炭转化、农药和纺织工业的废水中发现的污染物。炼化废水的主要污染物有酚类、多环芳烃(PAHs)和重金属。在这些有毒污染物中,酚类被认为是最有害的,当然也是最难去除的。酚类化合物在较低浓度下是有毒的。由于它们的浓度很低,所以最适合的去除方法是微生物法。本文对苯酚的生物降解进行了综述。苯酚的降解是大量微生物(包括细菌、真菌和放线菌)活动的结果。有报道称,许多微生物能够通过多种酶的作用降解苯酚。这些酶包括加氧酶、羟化酶、过氧化物酶、酪氨酸酶、氧化酶等。在不同的生物反应器中进行了批量实验。微生物使用纯培养物对有机化学品进行生物降解可产生有毒中间体。这个问题可以通过使用具有更广泛的代谢物降解特性的混合培养来克服。本文还介绍了苯酚生物降解的一些新技术,如不同固定化、FISH(荧光原位杂交)分析、纳米生物技术等。关键词:苯酚,生物降解,微生物,酶,固定化,FISH分析,纳米生物技术
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引用次数: 0
LABORATORY BIODEGRADATION OF POTENTIAL CELLULOSE WASTES GENERATED DURING LONG-TERM MANNED SPACE MISSIONS 长期载人航天任务中产生的潜在纤维素废物的实验室生物降解
Pub Date : 2019-03-31 DOI: 10.32006/eeep.2019.1.7178
H. Najdenski, V. Ilyin, P. Angelov, V. Hubenov, D. Korshunov, V. Kussovski, L. Dimitrova, I. Simeonov
Anaerobic microbial biodegradation of cellulose containing wastes generated during the long-term manned space missions is a key technological approach for resolving the problem of wastes accumulated onboard of the spacecraft. Herewith are presented data on the possibilities of structurally stable multispecies microbial consortia originating from methanogenic bioreactors to decompose different cellulose substrates – filter paper, medical gauze and vegetable mix as potential cellulose wastes during space missions. The rates of biodegradation processes carried out at mesophilic and thermophilic conditions are not only measured and compared but are providing new opportunities for development of technology for microbial biodegradation of cellulose-rich organic wastes. Moreover, the studies have shown that paper and gauze hydrolysis with the help of cellulolytic bacterial communities can be an effective component of utilization of cosmonaut hygiene items. Biodegradation of plant wastes by community of anaerobic bacteria is promising and applicable tool also under terrestrial conditions. Light microscopy of bacterial communities reveals the presence of Gram-positive spore forming bacilli (short and long forms, bipolar and dividing cells) and typical clostridia forms.
长期载人航天飞行过程中产生的含纤维素废弃物的厌氧微生物降解是解决航天器废弃物积累问题的关键技术途径。以下是关于产甲烷生物反应器产生的结构稳定的多物种微生物联合体在空间任务期间分解不同纤维素基质——滤纸、医用纱布和蔬菜混合物——作为潜在纤维素废物的可能性的数据。在中温和亲热条件下进行的生物降解过程的速率不仅被测量和比较,而且为开发富含纤维素的有机废物的微生物生物降解技术提供了新的机会。此外,研究表明,在纤维素水解菌群的帮助下,纸和纱布的水解可以成为宇航员卫生用品利用的有效组成部分。在陆地条件下,利用厌氧菌群对植物废弃物进行生物降解是一种很有前途的可行方法。光镜下细菌群落显示革兰氏阳性芽孢形成杆菌(短型和长型,双极性和分裂细胞)和典型梭状芽孢杆菌的存在。
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引用次数: 2
INFLUENCE OF THE MAGNETIC FIELD ON THE LIVING ORGANISMS AND HUMANS 磁场对生物和人类的影响
Pub Date : 2019-03-31 DOI: 10.32006/EEEP.2019.1.1725
S. Naydenov, Sonia Petkova, P. Getsov, G. Mardirossian
The question of the influence of magnetism on biological objects for years has been a debate with many contradictory opinions. The article provides a brief overview of publications and various and contradictory views on the influence of magnetic fields on living organisms and humans. An explanation is sought for findings made in various scientific studies, as well as an answer to the question: Can a permanent magnetic field be useful for our health and under what conditions?Key words: magnetic field, health, diagnostic, physiotherapy, physiological response
多年来,磁性对生物物体的影响问题一直是一个有许多相互矛盾意见的争论。本文简要概述了有关磁场对生物体和人类影响的各种出版物和相互矛盾的观点。对各种科学研究的发现进行解释,并回答这个问题:永久磁场对我们的健康有用吗?在什么条件下有用?关键词:磁场,健康,诊断,物理治疗,生理反应
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引用次数: 0
AN EXPERIMENTAL STUDY FOR THE CHARACTERIZATION OF BIOGAS PRODUCTION FROM COW DUNG AND DROPPINGS 牛粪产沼气特性的实验研究
Pub Date : 2019-03-20 DOI: 10.32006/eeep.2019.1.5462
Younoussa Moussa Baldé, Cellou Kanté, S. Diop, S. Tebbani
The present work is an account of an ongoing work on biogas production from animal wastes at LEREA (Laboratoire d’enseignement et de recherche en énergétique appliquée) in Mamou, Guinea. The work consists of biogas production from anaerobic digestion and co-digestion of cow dung and droppings. We focus in this report on the determination of the physico-chemical characteristics of the experimental setup. We have carried out three experiments of anaerobic digestion each one lasting 45 days at mesophilic temperature (temperature was maintained in the range 27°C - 28°C). Biogas - 28.4 liters have been obtained from droppings, 22.6 liters from cow dung and 38.7 liters from co-digestion of the previous two wastes. The following physico-chemical characteristcs were observed for cow dung: humidity 43%, dry matter 20.83%, organic matter 57%, density 625kg/m3, carbon content 31%, nitrogen content 1.46%, nitrogen-carbon ratio 21/30. For droppings we measured: humidity 35%, dry matter 65%, organic matter 62%, density 250 kg/m3, carbon content 36%, nitrogen level 1.83%. This characterization was carried out on a sample of 3 g of each type of substrate. These results agree with those of the literature that we were able to compare with.Keywords: anaerobic digestion; anaerobic co-digestion; physico-chimical characterization; cow dung weste; droppings weste; methanation; animal waste
目前的工作是在几内亚马穆的leea ( )进行的关于利用动物粪便生产沼气的研究工作。这项工作包括通过厌氧消化和牛粪和粪便的共消化产生沼气。在本报告中,我们着重于确定实验装置的物理化学特性。我们进行了三次厌氧消化实验,每次持续45天,温度保持在27°C - 28°C范围内。沼气——从粪便中获得28.4升,从牛粪中获得22.6升,从前两种废物的共消化中获得38.7升。牛粪的理化特征为:湿度43%,干物质20.83%,有机质57%,密度625kg/m3,碳含量31%,氮含量1.46%,氮碳比21/30。测定粪便湿度35%,干物质65%,有机物62%,密度250 kg/m3,碳含量36%,氮含量1.83%。这种表征是在每种衬底的3g样品上进行的。这些结果与我们能够比较的文献一致。关键词:厌氧消化;厌氧co-digestion;physico-chimical表征;西边是牛粪;粪便weste;甲烷化;动物粪便
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引用次数: 2
PARAMETERS OPTIMIZATION FOR INCREASED INTRACELLULAR INULINASE ACTIVITY OF A YEAST STRAIN 提高酵母菌胞内菊粉酶活性的参数优化
Pub Date : 2019-03-20 DOI: 10.32006/eeep.2019.1.6270
L. Kabaivanova, Adriana Goushterova, M. Brazkova, P. Grozdanov, E. Chorukova, A. Krastanov
This study reveals the selection of a yeast strain, possessing inulinase activity and finding the optimal conditions of cultivation. Intra- and extracellular activity assay was performed after cultivation on media, containing inulin as a sole source of carbon. Optimization of the cultivation conditions was carried out for establishing the favorable conditions for biosynthesis of inulinase. Modifying the physicochemical and nutritional parameters of a cultivation process lead to major improvement of the enzyme activity. Highest intra- and extracellular inulinase activity was registered when 1.5% inulin was used, 5 % inoculum, temperature 28°C, pH=6.5 and agitation of 200rpm. The selected strain Kluyveromyces sp. C showed higher values for the intracellular inulinase activity, making it suitable for immobilization and further use.Key words: Kluyveromyces sp., inulinase activity, parameters optimization
本研究筛选出了一株具有菊粉酶活性的酵母菌株,并找到了最佳培养条件。在含菊粉作为唯一碳源的培养基上培养后进行细胞内和细胞外活性测定。对培养条件进行了优化,为菊粉酶的生物合成创造了有利条件。修改培养过程的理化和营养参数可显著提高酶的活性。当菊粉浓度为1.5%,接种量为5%,温度28℃,pH=6.5,搅拌转速为200rpm时,细胞内和细胞外菊粉酶活性最高。所选菌株Kluyveromyces sp. C具有较高的胞内菊粉酶活性,适合固定化和进一步利用。关键词:克鲁维菌;菊粉酶活性;参数优化
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引用次数: 0
期刊
Ecological Engineering and Environment Protection
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