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CARBON MATERIALS FROM WASTE BIOMASS AS ANTI-BACTERIAL AIR FILTERS 从废弃生物质中提取碳材料作为抗菌空气过滤器
Pub Date : 2023-06-18 DOI: 10.32006/eeep.2023.1.6267
Georgi L. Georgiev, V. Hubenov, I. Stoycheva, Rumyana T. Eneva, B. Petrova, U. Szeluga, S. Pusz, Marieta Belcheva, B. Tsyntsarski
Carbon materials from almond shells were developed for the adsorption of microorganisms from air. Samples were processed by means of a one-step process – high-temperature hydro-pyrolysis. Studies were carried out in a wide temperature range of 600-900 °C. As a result, carbonates with predominant micro- and mesopores were obtained. The samples were impregnated with zinc, silver, iron and copper 5% by weight. The obtained samples were characterized by XDR, BET, scanning electron spectroscopy and elemental analysis. The final products are distinguished by a moderate surface and the presence of nanosized metal particles. The antibacterial properties of the activated carbon composites were examined using standard methodology under dynamic contact conditions and Escherichia coli K12 as test microorganism. All tested composite materials exhibit strong antibacterial properties after 48 h of contact with microbial cells. Thus the application of these materials in filtering system will be possible solution for successful reduction of microbial cell number. It is assumed that a similar effect can be achieved in an air environment.
以杏仁壳为原料研制了吸附空气中微生物的碳材料。样品采用高温加氢热解一步法处理。研究在600-900°C的宽温度范围内进行。得到了微孔和中孔占优势的碳酸盐岩。样品被锌、银、铁和铜按重量的5%浸渍。用XDR、BET、扫描电子能谱和元素分析对所得样品进行了表征。最终产品的特点是温和的表面和纳米尺寸的金属颗粒的存在。采用标准方法,以大肠杆菌K12为试验微生物,在动态接触条件下考察了活性炭复合材料的抗菌性能。所有测试的复合材料在与微生物细胞接触48小时后都表现出很强的抗菌性能。因此,这些材料在过滤系统中的应用将是成功减少微生物细胞数量的可能解决方案。假定在空气环境中也能达到类似的效果。
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引用次数: 0
GROSS ALPHA AND BETA ACTIVITY IN MINERAL WATER FROM SOUTHWEST BULGARIA 保加利亚西南部的矿泉水中总的α和β活性
Pub Date : 2023-06-18 DOI: 10.32006/eeep.2023.1.7782
E. Geleva, Svetoslav Dimitrov, N. Goutev, Verzhinia Variyska, D. Tonev
The natural radioactivity levels of mineral water from certain most frequently used sources in Southwest Bulgaria were determined. The mineral water was investigated with regard to gross alpha and beta activity to determine whether the activity concentrations are below levels at which no further action is required. The measurements were made by Liquid Scintillation Spectrometer. The gross alpha and beta activity varied from ≤ 0.003 Bq/L to 0.671 Bq/L and from 0.054 and to 0.375 Bq/L, respectively. The values obtained in this study were compared with data reported by other authors in different countries and with reference values accepted for drinking water.The annual effective doses were calculated for all investigated waters for adult inhabitants assuming yearly consumption of 730 litres. The results show that the annual effective dose of ingestion of these water samples excluding one, are below the individual dose criterion of 100 µSv/y according to the World Health Organization recommendations.
对保加利亚西南部某些最常用水源的矿泉水的天然放射性水平进行了测定。对矿泉水的总α和β活性进行了调查,以确定活性浓度是否低于无需采取进一步行动的水平。采用液体闪烁光谱仪进行测量。总α和β活性变化范围分别为≤0.003 ~ 0.671 Bq/L和0.054 ~ 0.375 Bq/L。本研究获得的数值与不同国家其他作者报告的数据以及饮用水公认的参考值进行了比较。假定成年居民年消费量为730升,计算了所有调查水域的年有效剂量。结果表明,除一个水样外,这些水样的年有效摄入剂量均低于世界卫生组织建议的100µSv/y的个人剂量标准。
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引用次数: 0
BIOCIDAL ACTION OF ANOLYTE 阳极液的杀菌作用
Pub Date : 2023-06-18 DOI: 10.32006/eeep.2023.1.6876
S. Karadzhov, T. Popova, I. Ignatov, L. Dimitrova, I. Nikolova, V. Kussovski, H. Naidenski, G. Gluhchev
The anolyte, obtained during electrochemical activation of low mineralized water solutions possesses a strong biocidal action due to its physical-chemical properties. This study presents a brief report for the effect of anolyte application to samples of different kind of microorganisms. An idea of the physical-chemical processes taking place in the anode chamber of an electrolyzer is present. The changes in the parameters pH, ORP and the presence of active chlorine during the time and at different storage conditions of the anolyte were examined. The experiments showed their stability and preservation of biocidal activity for a period longer than 9 months. Experimental results from investigations of the biocidal effect of anolyte carried out in different laboratories are present.
在低矿化度水溶液的电化学活化过程中得到的阳极液,由于其物理化学性质,具有很强的杀菌作用。本文简要介绍了阳极液在不同微生物样品中的应用效果。介绍了在电解槽阳极室中发生的物理化学过程。考察了阳极液在不同贮存条件下pH、ORP、活性氯含量等参数的变化。实验结果表明,其稳定性和杀菌活性的保存时间超过9个月。本文介绍了在不同实验室进行的阳极液杀菌效果研究的实验结果。
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引用次数: 0
MICROBIAL BIOREMOVAL OF DIVALENT TOXIC METALS 二价有毒金属的微生物去除
Pub Date : 2023-06-18 DOI: 10.32006/eeep.2023.1.2735
I. Bida, O. Havryliuk, V. Hovorukha, G. Gladka, O. Tashyrev
The problems of polymetallic wastewater treatment from mining enterprises as well as the accumulation of organic waste are acute worldwide. The application of any existing methods of wastewater purification is ineffective and impossible due to the huge volumes and high concentrations of metals. Similarly, modern methods are ineffective for the treatment of huge amounts of organic waste. Therefore, there is a necessity to develop novel environmental biotechnologies providing the simultaneous degradation of organic waste and detoxification of toxic metals. The purpose of the work was to theoretically substantiate and experimentally confirm the possibility of toxic divalent cations removal using dissimilatory sulfate reduction via anaerobic fermentation of ecologically hazardous model organic waste. Colorimetric and potentiometric methods were used for pH and redox potential measurement; volumetric and chromatographic methods – to control volume and composition of synthesized gas; permanganate method – to determine the concentration of dissolved organic carbon (DOC); photocolorimetric method via the qualitative reaction with Nessler’s reagent was used to determine the concentration of ammonium ions. The Co2+ and Ni2+ content in medium was determined by a colorimetric method with 4-(2-pyridylazo)resorcinol (PAR). Fermentation parameters were calculated with the use of mathematical and statistical ones. Modified Postgate B medium with different sources of carbon and energy (potatoes, alanine, and meat) was used for cultivation of dissimilatory sulfate reducing bacteria. The anaerobic microbiome obtained from the sludge of methane tanks showed high efficiency to remove Co2+ and Ni2+ from the liquid medium. The highest efficiency (100% in 9 days) was observed when alanine was used as a source of carbon and energy. The slowest metal precipitation process occurred using meat (20 days). Also, the use of a protein substrate did not provide the expected alkalinization of the medium, which could significantly accelerate the process of metal precipitation. The precipitation of cobalt and nickel cations during the hydrogen fermentation of potato starch was complicated by acidification of the medium, but it was equally effective when the pH was adjusted. The proposed approach, the slow dissimilatory sulfate reduction, due to the sparingly soluble calcium sulfate as electron acceptor, can be used as a basis for the development of new biotechnologies for the treatment of wastewater contaminated with divalent heavy metals with the simultaneous treatment of ecologically hazardous compounds.
矿山企业多金属废水的处理和有机废物的积累问题在世界范围内都很突出。由于大量和高浓度的金属,任何现有的废水净化方法的应用都是无效和不可能的。同样,现代方法对于处理大量的有机废物也是无效的。因此,有必要开发新的环境生物技术,同时提供有机废物的降解和有毒金属的解毒。本研究的目的是在理论上证实和实验上证实利用厌氧发酵对生态有害的模型有机废物进行异化硫酸盐还原去除有毒二价阳离子的可能性。pH和氧化还原电位的测定采用比色法和电位法;体积法和色谱法。控制合成气体的体积和组成高锰酸盐法-测定溶解有机碳(DOC)浓度;采用奈斯勒试剂定性反应的光比色法测定了铵离子的浓度。用4-(2-吡啶偶氮)间苯二酚(PAR)比色法测定培养基中Co2+和Ni2+的含量。利用数理统计法计算发酵参数。采用不同碳源和能量源(马铃薯、丙氨酸和肉类)的改良Postgate B培养基培养异化硫酸盐还原菌。从甲烷罐污泥中获得的厌氧微生物群对液体介质中Co2+和Ni2+的去除效果良好。以丙氨酸为碳源和能量源时,效率最高(第9天达到100%)。使用肉类的金属析出过程最慢(20 d)。此外,蛋白质底物的使用并没有提供预期的碱化培养基,这可能会显著加速金属沉淀的过程。培养基酸化使马铃薯淀粉氢发酵过程中钴和镍阳离子的沉淀变得复杂,但调节pH值同样有效。所提出的方法,缓慢的异化硫酸盐还原,由于少溶硫酸钙作为电子受体,可以作为开发新的生物技术的基础,用于处理二价重金属污染的废水,同时处理生态有害化合物。
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引用次数: 0
BIOKINETIC CHARACTERIZATION OF FERROPLASMA ACIDIPHILUM 嗜酸铁原体的生物动力学特性
Pub Date : 2023-06-18 DOI: 10.32006/2023.1.0516
J. Daoud, D. Karamanev
Abstract. Recently a mixed culture dominated by the iron-oxidizing microorganisms Leptospirillum and Ferroplasma has been used in a large-scale microbial fuel cell for electrical power generation. There are many factors that affect the kinetics of iron oxidation by the mixotroph Ferroplasma acidiphilum. This study investigated the effects of pH, temperature, and substrate and yeast extract concentrations in order to arrive at kinetically favorable operating conditions with minimal jarosite precipitation. Furthermore, F. acidiphilum was cultured with Leptospirillum sp. in order to determine its viability as a species capable of limiting the organic by-products of chemolithotrophic microbial growth. Bacterial characterization in the culture was accomplished using fluorescent in situ hybridization (FISH). It was found that the ferrous iron oxidation was most favorable at yeast extract concentration of 0.02% (w/v), pH of 1.6, temperature of 35 oC and an initial ferrous iron concentration of 1 g/L yielding a maximum specific growth rate of 0.0351-0.042 h-1. Moreover, F. acidiphilum displayed a symbiotic relationship with its chemolithotrophic counterpart, Leptospirillum sp. in that they were able to utilize the metabolic organic products of the chemolithotroph and limit the organic concentration to ~20 ppm total organic carbon (TOC), well below the threshold concentration of 250 ppm for chemolithotroph activity.
摘要最近,以铁氧化微生物钩端螺旋体和铁原体为主的混合培养已被用于大规模的发电微生物燃料电池。影响嗜酸铁原体氧化铁动力学的因素很多。本研究考察了pH、温度、底物和酵母提取物浓度的影响,以达到动力学上有利的操作条件,使黄钾铁矾沉淀最少。此外,嗜酸F.与钩端螺旋体一起培养,以确定其作为一种能够限制趋化石营养微生物生长的有机副产物的生存能力。利用荧光原位杂交(FISH)完成了培养中的细菌鉴定。结果表明,酵母浸膏浓度为0.02% (w/v)、pH为1.6、温度为35℃、初始亚铁浓度为1 g/L时,亚铁氧化效果最佳,最大比生长速率为0.0351 ~ 0.042 h-1。此外,嗜酸F.与它的化化营养对生物细螺旋体(Leptospirillum sp.)表现出共生关系,它们能够利用化化营养菌的代谢有机产物,并将有机浓度限制在~ 20ppm的总有机碳(TOC),远低于化化营养菌活性的阈值浓度250ppm。
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引用次数: 0
CELLULOLYTIC MICROORGANISMS: AEROBIC, MICROAEROPHILIC, ANAEROBIC BACTERIA AND MICROBIAL CONSORTIA (Part II) 纤维素分解微生物:好氧、嗜微氧、厌氧细菌和微生物群落(第二部分)
Pub Date : 2023-06-18 DOI: 10.32006/eeep.2023.1.3653
Y. Gocheva, Lyudmila L Dimitrova, V. Hubenov, L. Kabaivanova, P. Angelov, I. Simeonov, H. Najdenski
In nature, cellulose, lignocellulose and lignin are major sources of plant biomass therefore their recycling is indispensable for the carbon cycle. The synergistic action of a variety of microorganisms is needed for recycling lignocellulosic materials. The capacities of microorganisms to assimilate complex carbohydrates, such as cellulose,hemicellulose and lignin, depend on the ability to produce the enzymes that work synergically. Populations growing in compost piles consist mainly of bacteria (including actinobacteria) and fungi. Polymers such as hemicellulose, cellulose, and lignin are only degraded once the more easily degradable compounds have been consumed. Afterwards, the lignocellulosic materials are partly transformed into humus. In the present review, numerous studies on the isolation of cellulose-degrading bacteria and fungi, their identification, enzymatic activities, and their ability to grow in thepresence of lignocellulose and components of these industrial waste streams (phenolic compounds, sulfides, and dyes are analyzed and discussed. This is of particular interest to design future studies to isolate those bacteria that can specifically degrade cellulose matrix and more recalcitrant components such as lignin and aromatic lignin degradation products. Cultivation and characterization of microorganisms alone is not adequate without preservation techniques that do not alter the morphology, physiology or genetics of pure strains. Careful preservation is imperative for future research, teaching and industrial applications.
在自然界中,纤维素、木质纤维素和木质素是植物生物量的主要来源,因此它们的循环利用对于碳循环是必不可少的。回收木质纤维素材料需要多种微生物的协同作用。微生物吸收复杂碳水化合物(如纤维素、半纤维素和木质素)的能力取决于其产生协同作用的酶的能力。在堆肥堆中生长的种群主要由细菌(包括放线菌)和真菌组成。聚合物如半纤维素、纤维素和木质素只有在更容易降解的化合物被消耗后才会被降解。然后,部分木质纤维素物质转化为腐殖质。本文对纤维素降解细菌和真菌的分离、鉴定、酶活性以及它们在木质纤维素和这些工业废水成分(酚类化合物、硫化物和染料)存在下的生长能力进行了分析和讨论。这对于设计未来的研究以分离那些能够特异性降解纤维素基质和更顽固的成分(如木质素和芳香木质素降解产物)的细菌具有特别的意义。如果没有不改变纯菌株的形态、生理或遗传学的保存技术,单独培养和鉴定微生物是不够的。仔细保存对未来的研究、教学和工业应用至关重要。
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引用次数: 0
METHODOLOGY FOR A KINEMATIC MODEL OF SEISMIC EARLY WARNING SYSTEM ABOUT CRITICAL INFRASTRUCTURE 关键基础设施地震预警系统运动学模型的方法学
Pub Date : 2023-06-18 DOI: 10.32006/eeep.2023.1.5461
B. Ranguelov, Garo Mardirosian
The destructive earthquakes generated huge losses and human victims. As for the moment the time of impending strong seismic event is unpredictable, during the last years – new global innovation is under development – the seismic early warning systems (SEWS). All of them signalize the population and/or governmental bodies and after the earthquake starts just providing information that the seismic event is fact, to expect stronger shakings and some very fast measures to be performed – for example NPP’s reactors stop, as well as superfast trains, chemical production, gas and oil pipeline disconnection, etc. In lucky cases some people can react (especially in the areas of low buildings) and to evacuate effective in the time domain of few seconds, thus saving their lives. Such system is in operation in Japan since 2007 and some other countries also develop their own SEWS. The present investigation is targeted to the methodology development of so called kinematic SEWS. They are using a fundamental property of the body seismic waves P and S. As it is well known from the theory, the P-waves are faster and less destructive than S-waves. This property gives the possibility to use the detection of the P waves as important signal that after a while (the time domain depends on the distance between the seismic source and the investigated site) the slower (1.41 times) more destructive S waves will arrive and struck the infrastructure. Our previous experience of the developed SEWS (for example for Baku and Venice) gives the possibility to upgrade the modeling methodology extending to the formalized algorithm and accuracy estimations, especially for the critical infrastructure (for example NPP).
破坏性的地震造成了巨大的损失和人员伤亡。至于即将到来的强烈地震事件的时间是无法预测的,在过去的几年里——新的全球创新正在开发中——地震预警系统(SEWS)。所有这些都是向民众和/或政府机构发出信号,在地震开始后,只是提供地震事件是事实的信息,期待更强的震动,并采取一些非常快速的措施——例如核电站的反应堆停止,以及超高速列车,化学生产,天然气和石油管道断开等。在幸运的情况下,一些人可以做出反应(特别是在低层建筑区域),并在几秒钟的时间内有效撤离,从而挽救了他们的生命。自2007年以来,这种系统在日本开始运行,其他一些国家也在开发自己的SEWS。目前的调查是针对所谓的运动学SEWS的方法论发展。他们利用了人体地震波P和s的基本特性。正如从理论中众所周知的那样,P波比s波更快,破坏性更小。这一特性使探测到的纵波成为一种重要信号成为可能,一段时间后(时域取决于震源和调查地点之间的距离),更慢(1.41倍)更具破坏性的纵波将到达并袭击基础设施。我们以前开发SEWS的经验(例如巴库和威尼斯)提供了升级建模方法的可能性,扩展到形式化算法和精度估计,特别是对于关键基础设施(例如NPP)。
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引用次数: 0
MINERAL WATERS IN BULGARIA 保加利亚的矿泉水
Pub Date : 2023-06-18 DOI: 10.32006/eeep.2023.1.1726
A. Sadovski
The present study aims to review the sources of mineral water in Bulgaria, as well as the various aspects of their use as an energy source, benefit for human health and the national economy. Several literary sources on the subject are cited. Statistical analysis was performed and data were summarized about their physical and chemical characteristics, and especially to find distribution by temperature. The production of cheap thermal energy from the geothermal plants contribute to the reduction of the environment pollution, which is of great importance for the country. The extremely good bio-climatic resources in the country provide a base for the prospective development of the balneological branch. The barriers to geothermal development in the country are shown. It is concluded that the importance of geothermal energy from mineral waters in Bulgaria is underestimated.
本研究的目的是审查保加利亚的矿泉水来源,以及将矿泉水作为一种能源,对人类健康和国民经济有益的各个方面。引用了关于这个主题的几个文学来源。通过统计分析,总结了它们的理化特性,特别是温度分布。地热发电厂生产的廉价热能有助于减少环境污染,这对国家具有重要意义。我国得天独厚的生物气候资源为该学科的未来发展提供了基础。显示了该国地热开发的障碍。结论是,保加利亚矿泉水地热能的重要性被低估了。
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引用次数: 1
THEORETICAL COMPARATIVE STUDIES OF THE BIOGAZ YIELDS FROM ONE-STAGE AND TWO-STAGE ANAEROBIC DIGESTION PROCESSES 一段和两段厌氧消化过程沼气产量的理论比较研究
Pub Date : 2022-11-20 DOI: 10.32006/eeep.2022.2.4455
Ning Pan, Haoping Wang, Yang Tian, E. Chorukova, I. Simeonov, N. Christov
This paper deals with the theoretical comparison of biogas and energy yields of one-stage anaerobic digestion processes (OSAD) with biomethane production and two-stage anaerobic digestion processes (TSAD) producing biohydrogen and biomethane. The comparative study of the biogas yield from OSAD and TSAD systems is performed on the base on mathematical models obtained in our previous papers. The possible maximal yields of biohydrogen and biomethane are calculated by the static characteristics and extremum points of both systems. Simulation results suggest that in comparison to OSAD, the increase in biogas (biohydrogen and biomethgane) production of TSAD can reach to 75.18%. The energy produced from two-stage anaerobic digestion processes is 1.32 to 1.486 times greater than those from one-stage anaerobic digestion processes (depending of the inlet organics concentrations), which means TSAD is a better choice considering biogas and energy production.
本文对产生生物甲烷的一段厌氧消化工艺(OSAD)和产生生物氢和生物甲烷的两段厌氧消化工艺(TSAD)的沼气和产能进行了理论比较。对OSAD系统和TSAD系统的沼气产量进行了比较研究,并基于我们之前论文中得到的数学模型。根据两种体系的静态特性和极值点,计算出生物氢和生物甲烷可能的最大产率。模拟结果表明,与OSAD相比,TSAD的沼气(生物氢和生物甲烷)产量可提高75.18%。两段厌氧消化过程产生的能量是一段厌氧消化过程产生的能量的1.32 ~ 1.486倍(取决于进口有机物浓度),这意味着考虑沼气和能源生产,TSAD是更好的选择。
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引用次数: 0
REGIMES FOR CLOSE-TO-NATURE MANAGEMENT OF THE HIGH-MOUNTAIN PASTURES ON THE TERRITORY OF BELASITSA NATURE PARK belasitsa自然公园境内高山牧场的接近自然管理制度
Pub Date : 2022-11-20 DOI: 10.32006/eeep.2022.2.6872
S. Savev
The high mountain and subalpine treeless part of the territory of Belasitsa Nature Park is occupied by herbaceous and shrub communities. Almost the entire part of the mountain roof is represented by a natural habitat 4060 "Alpine and boreal ericoid communities", with Siberian juniper being the dominant species in the communities. The current state of the vegetation resulted from the lack of economic activities in the treeless area in the last 25 years. Surveys to date have revealed significant change resulting in a reduction in the total area of grassland habitats as a result of colonization with ericoid scrub. Considering the trend of natural succession processes, the management in this area should focus to the restoration and maintenance of grass vegetation except in places with species of high conservation value. In this case, grazing can be a natural tool slowing down this undesired succession. The grassland communities in the highlands of our mountains are semi-natural and this tool could prevent them from becoming entirely juniper scrub, which would result in the loss of remaining natural habitats of conservation value. Research data show that in the past, the load of livestock to the pastures in the area was less than their capacity, i.e. pastures in the park have not been overcrowded. This fact indicates that more intense use of the pastures in the future will not provoke a danger of disturbing plant diversity. Habitat 4060 in Belasitsa occupies an area of 1023.97 ha, or 98% of the total 1037.48 ha of treeless high mountain area, with the remaining 13.51 ha being pastures of conservation importance. The grasslands of conservation importance are represented by three types of natural habitats, which, as a result of juniper succession, are highly fragmented, represented by several separate small areas of several hectares each. Two areas for pastures have been designated along the land borders between Samuilovo and Kolarovo. It is recommended that grazing be done on a rotational basis in the two areas.
Belasitsa自然公园境内的高山和亚高山无树部分被草本和灌木群落所占据。几乎整个山顶都是一个自然栖息地4060“高山和北方ericoid群落”,其中西伯利亚杜松是群落中的优势物种。目前的植被状况是由于过去25年来无树地区缺乏经济活动造成的。迄今为止的调查显示,由于灌丛植物的殖民化,草地栖息地总面积发生了重大变化,导致面积减少。考虑到自然演替过程的趋势,除具有较高保护价值的物种外,该区的管理应侧重于草地植被的恢复和维护。在这种情况下,放牧是一种自然的工具,可以减缓这种不受欢迎的演替。我们山区高地的草地群落是半自然的,这个工具可以防止它们完全变成杜松丛,这将导致剩余的具有保护价值的自然栖息地的丧失。研究数据表明,过去,该地区牧场的牲畜负荷小于其容量,即公园内的牧场没有过度拥挤。这一事实表明,未来更密集地利用牧场不会引起干扰植物多样性的危险。Belasitsa的栖息地4060占地1023.97公顷,占无树高山总面积1037.48公顷的98%,其余13.51公顷为具有重要保护意义的牧场。具有重要保护意义的草地由三种类型的自然栖息地代表,由于杜松的演替,这些栖息地高度分散,由几个独立的小区域代表,每个小区域几公顷。沿着萨穆伊洛沃和科拉罗沃之间的陆地边界划定了两个牧场。建议在这两个地区轮流放牧。
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引用次数: 0
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Ecological Engineering and Environment Protection
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