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ANTISENSE OLIGONUCLEOTIDES AND RADIONUCLIDES – PERSPECTIVES 反义寡核苷酸和放射性核素-观点
Pub Date : 2021-06-10 DOI: 10.32006/eeep.2021.1.1724
Katya B. Popova, M. Mincheva, M. Yavahchova, N. Goutev, D. Tonev
Over the past decade, antisense technology has successfully established itself as an entirely innovative platform for research and creation of new therapies. Significant advances in the design of antisense oligonucleotides, as well as a deeper understanding of their mechanisms of action, have led to their successful clinical application in many RNA-targeted therapies. In addition, their potential for in vivo imaging by radiolabeling has been identified. Here are discussed the prospects for the use of antisense oligonucleotides in nuclear medicine and highlighted some of the advantages and disadvantages of labelling them with radionuclides.
在过去的十年里,反义技术已经成功地建立了自己作为一个完全创新的研究和创造新疗法的平台。反义寡核苷酸设计的重大进展,以及对其作用机制的更深入了解,导致它们在许多rna靶向治疗中成功的临床应用。此外,它们通过放射性标记在体内成像的潜力已被确定。本文讨论了反义寡核苷酸在核医学中的应用前景,并重点介绍了用放射性核素标记它们的一些优点和缺点。
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引用次数: 0
INVESTIGATING THE HISTORICAL CORRELATION BETWEEN ATMOSPHERIC CARBON DIOXIDE CONCENTRATION AND GLOBAL TEMPERATURE CHANGE 研究大气二氧化碳浓度与全球温度变化之间的历史相关性
Pub Date : 2021-06-10 DOI: 10.32006/eeep.2021.1.0516
Kneev Sharma, D. Karamanev
Understanding the fundamental relationship between atmospheric carbon dioxide concentration and temperature rise is essential for tackling the problem of climate change that faces us today. Misconceptions regarding the relationship are widespread due to media and political influences. This investigation aims to address the popular misconception that CO2 concentration directly and naturally leads to global temperature rise. While anthropogenic CO2 emissions seem to affect the rising global atmospheric temperature with a confidence of 95%, it falters when the historical relationship using ice core data is studied. This investigation uses two statistical approaches to determine an accurate range and direction for this important relationship. Through a combined approach, it was found that historically CO2 concentration in the last 650 000 years lags global temperature rise by 1020-1080 years with a maximum correlation coefficient of 0.8371-0.8372. This result is important for the investigation of climate change.
了解大气中二氧化碳浓度与气温上升之间的基本关系,对于解决我们今天面临的气候变化问题至关重要。由于媒体和政治的影响,对两国关系的误解普遍存在。这项调查的目的是解决普遍的误解,即二氧化碳浓度直接和自然地导致全球气温上升。虽然人为二氧化碳排放似乎以95%的置信度影响全球大气温度的上升,但当使用冰芯数据研究历史关系时,这种影响就不那么明显了。这项调查使用两种统计方法来确定这一重要关系的准确范围和方向。综合分析发现,近65万年CO2浓度滞后全球升温1020 ~ 1080年,最大相关系数为0.8371 ~ 0.8372。这一结果对研究气候变化具有重要意义。
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引用次数: 0
CURRENT AND FUTURE WAYS TO CLOSED LIFE SUPPORT SYSTEMS: VIRTUAL MELISSA CONFERENCE, GHENT (B) (3-5 /11/2020). A REVIEW 封闭生命支持系统的当前和未来方法:虚拟梅丽莎会议,根特(b)(3-5 /11/2020)。回顾
Pub Date : 2021-06-10 DOI: 10.32006/eeep.2021.1.2535
C. Lasseur, M. Mergeay
The 3-days international virtual MELiSSA conference (November 3 to 5, 2020) about the closed life support systems was organized by the University of Ghent and IDDUP in collaboration with ESA/ESTEC (European Space Agency) and MELiSSA Foundation, and welcomed around 150 papers. The first day addressed the welcomed contributions of the 5 major space agencies, the plants & photosynthetic microorganisms used for life support in space, the simulation of planetary ecosystems as well as MELiSSA inspired circular economy and education. The second day focused on Ground demonstration and analogue testing, Photobioreactors and photosynthetic microorganisms characterization, Modelling and Systems design, Organic wastes process and Metabolome where a real breakthrough was observed as well as in human microbiome in closed environments. The last day addressed: 1) the complex problematics of urine as a major source of nutrients, 2) Terrestrial synergies, and 3) some Physical, Chemical and Microbial contaminants linked to space research.
2020年11月3日至5日,由根特大学和IDDUP与ESA/ESTEC(欧洲航天局)和MELiSSA基金会合作,组织了为期3天的关于封闭生命支持系统的国际虚拟MELiSSA会议,共发表了约150篇论文。第一天讨论了5个主要空间机构的受欢迎的贡献,用于空间生命维持的植物和光合微生物,行星生态系统的模拟以及MELiSSA启发的循环经济和教育。第二天的重点是地面演示和模拟测试,光生物反应器和光合微生物表征,建模和系统设计,有机废物处理和代谢组,在封闭环境中观察到真正的突破,以及人类微生物组。最后一天讨论了:1)尿液作为主要营养来源的复杂问题,2)陆地协同作用,以及3)与空间研究有关的一些物理、化学和微生物污染物。
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引用次数: 5
INFLUENCE OF CLIMATE CHANGE ON THE SPREAD OF INVASIVE ALIEN PLANT SPECIES IN THE TERRITORY OF THE LOZENSKA MOUNTAIN 气候变化对洛真斯卡山区外来入侵植物物种传播的影响
Pub Date : 2021-06-10 DOI: 10.32006/eeep.2021.1.7882
Plamen Glogov, Dimitar Dimitrov
The paper presents a comparative analysis of the data on the main climatic components for three periods - 1921-1945; 1965-1975; 2000-2015 and their connection with the distribution of Invasive alien plant species (IAS) on the territory of Lozenska Mountain. Among the most important features of the mountain’s climate that favorably affect the spread of IAS are the two precipitation maximums and minimums and the period of drought. In response to climate and other environmental changes, IAS on the territory of the mountain are spread in two ways - by forming monodominant communities in extreme habitats or by integrating their populations into the local communities and gradually increasing their presence in them, accompanied by changing ecological conditions of the habitat. Four potential invasive alien plant species (PIAS) have been identified: Opuntia fragilis (Nutt.) Haw., Opuntia engelmannii Salm-Dyck ex Engelmann, Lupinus polyphylus Lindl., Impatiens baulfourii Hook.f., to which population monitoring and control measures need to be implemented.
本文对1921—1945年三个时期的主要气候成分资料进行了比较分析;1965 - 1975;2000-2015年洛赞斯卡山外来入侵植物的分布及其与外来入侵植物的关系有利于IAS传播的山区气候最重要的特征是两个降水最大值和最小值以及干旱期。为了应对气候和其他环境变化,山区境内的外来入侵物种以两种方式传播——在极端栖息地形成单一的群落,或者随着栖息地生态条件的变化,将其种群融入当地社区并逐渐增加其存在。4种潜在的外来入侵植物(PIAS)已被确定:山楂。, Opuntia engelmannii Salm-Dyck ex Engelmann, Lupinus polyphylus Lindl。英帕提斯·鲍福瑞·胡克。,需要实施人口监测和控制措施。
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引用次数: 2
GASEOUS FUEL OBTAINING VIA FERMENTATION OF ORGANIC LANDFILL WASTE 通过有机垃圾填埋场垃圾发酵获得的气体燃料
Pub Date : 2021-06-10 DOI: 10.32006/eeep.2021.1.3648
V. Hovorukha, O. Havryliuk, G. Gladka, Bida Iryna, Y. Danko, O. Shabliy, O. Tashyrev
Fossil carbon-containing fuel is currently one of the most common in industry and economy. The rapid depletion of reserves of this fuel makes it necessary to search for the alternative one. Landfills are a place where methane is spontaneously synthesized due to the decay of organic waste. Controlled and regulated fermentation of the landfill organics can provide biomethane as well as environmental bioremediation. The aim of the work was to study the patterns of methane fermentation of multi component organic waste and optimize the process to increase the efficiency of biomethane synthesis and waste decomposition. Colorimetric and potentiometric methods were used for pH and Eh measurement. Volumetric and chromatographic methods were applied to control volume and composition of synthesized gas. Fermentation parameters were calculated with the use of mathematical and statistical ones. The achievement of high efficiency of methane fermentation of organic waste due to the process regulation was shown. The modeling of unregulated fermentation of organic waste in landfills showed low efficiency of the process. It took 69 days. Weight of waste decreased only 5 times. Hydrogen yield was 5 L/kg of waste. Methane was not synthesized. The regular mass transfer, regulation of the process and waste grinding showed the greatest efficiency. Weight of waste decreased 20 times during only 14 days. Hydrogen yield was 27 L/kg, methane yield was 12 L/kg of waste. Thus, the absence of regulation caused long term decay of waste. The high efficiency is achieved due to regulation of the fermentation process. The results will serve as a basis for the development of industrial biotechnology for the utilization of organic waste to reduce the volume of existing landfills and produce methane energy. This will further allow bioremediation of contaminated areas, obtaining an alternative to fossil fuel biomethane.
含碳化石燃料是目前工业和经济中最常用的燃料之一。这种燃料储量的迅速枯竭使得有必要寻找替代燃料。垃圾填埋场是由于有机废物的腐烂而自发合成甲烷的地方。垃圾填埋场有机物的可控发酵不仅可以提供生物甲烷,还可以对环境进行生物修复。本研究旨在研究多组分有机废弃物的甲烷发酵规律,并对其进行工艺优化,以提高生物甲烷合成和废弃物分解效率。pH和Eh的测定采用比色法和电位法。采用体积法和色谱法控制合成气的体积和组成。利用数理统计法计算发酵参数。通过工艺调控,实现了有机废弃物甲烷高效发酵。通过对垃圾填埋场有机废弃物无调节发酵过程的模拟,发现该过程效率较低。整个过程花了69天。废物重量只减少了5倍。产氢量为5 L/kg废弃物。甲烷不是合成的。有规律的传质、有规律的工艺、有规律的废磨效率最高。仅在14天内,废物重量就减少了20倍。产氢量为27 L/kg,甲烷量为12 L/kg。因此,缺乏监管导致了废物的长期衰变。由于发酵过程的调节,实现了高效率。研究结果将作为开发利用有机废物的工业生物技术的基础,以减少现有垃圾填埋场的体积并产生甲烷能源。这将进一步允许污染地区的生物修复,获得化石燃料生物甲烷的替代品。
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引用次数: 0
GROWTH MODELS IN MICROBIAL ECOSYSTEMS - RESOURCE OR DENSITY DEPENDENCE? 微生物生态系统的生长模式——资源依赖还是密度依赖?
Pub Date : 2021-06-10 DOI: 10.32006/eeep.2021.1.6167
J. Harmand
This paper aims at discussing the two main modeling schemes that are used to describe dynamically the growth of microbial ecosystems, that are resource and density-dependent growth functions, respectively. Monod has been the first to hypothesize that this growth is, before all, an increasing saturated function of the main limiting substrate concentration. Contois assumed that the growth is not only a function of the substrate but also of the biomass density-itself, and thus the name « density-dependent ». In re examining their respective experiments (species used, conditions of experiments, mode of reactor functioning, measurement techniques), we try to understand the engines for a density-dependent phenomenon to appear. In particular, we refer to recent experiments where it was shown that density-dependent appeared as soon as the biomass structures into flocs or in the presence of filamentous bacteria even at relatively low concentrations. Based on this historical review of data, it is shown that density-dependent kinetics is not systematically a question of biomass density but rather related to its structure within the medium and to the mobility of microbial cells.
本文旨在讨论用于动态描述微生物生态系统生长的两种主要建模方案,即资源依赖和密度依赖的生长函数。莫诺德是第一个假设这种增长首先是主要限制底物浓度的饱和函数。Contois假设生长不仅是基质的函数,也是生物量密度本身的函数,因此被称为“密度依赖”。在重新检查他们各自的实验(使用的物种、实验条件、反应堆功能模式、测量技术)时,我们试图理解密度依赖现象出现的原因。特别是,我们参考了最近的实验,实验表明,即使在相对较低的浓度下,一旦生物质结构成絮凝体或丝状细菌存在,密度依赖性就会出现。基于这一历史回顾的数据,它表明,密度依赖动力学不是一个系统的生物量密度问题,而是与其在培养基中的结构和微生物细胞的流动性有关。
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引用次数: 0
ANAEROBIC DIGESTION OF WHEAT STRAW AND MICOBIOLOGICAL ASSESMENT OF THE RESULTED DIGESTATE 麦秸厌氧消化及其消化产物的微生物学评价
Pub Date : 2021-06-10 DOI: 10.32006/eeep.2021.1.4960
H. Najdenski, L. Dimitrova, Veselin Akivanov, V. Hubenov, Snejanka Mihailova, P. Grozdanov, M. Iliev, V. Kussovski, L. Kabaivanova, I. Simeonov
A study on the anaerobic digestion of wheat straw in a pilot scale bioreactor with an organic load of 2, 5, 7, 10 and 20 g/l and a process duration of 18 to 80 days was performed. The pilot bioreactor used has a computerized system for control and monitoring of various operational parameters – temperature, pH, biogas composition, etc. Total solids, total organics and volatile fatty acids were measured by standard methods and gas chromatography. Daily biogas yield and its main components (CH4, CO2, H2S) were analysed too. During the anaerobic digestion, different species of microorganisms have been isolated from the genera Bacillus, Pseudomonas, Enterococcus and Aeromonas, as well as the species Terribacillus halophilus. With a known pathogenic potential are described Pseudomonas sp., Enterococcus sp. and Aeromonas sp. Studies on the antimicrobial resistance of all isolated strains show resistance to ampicillin, amoxicillin, bacitracin, ceftriaxone, gentamicin and vancomycin. The cellulose degrading activity of some of the bacterial isolates, their pathogenic potential and antimicrobial resistance are discussed in detail in the light of the data on the mechanisms of proven resistance.
在有机负荷为2、5、7、10和20 g/l的中试生物反应器中进行了麦秸厌氧消化试验,试验时间为18 ~ 80 d。所使用的中试生物反应器有一个计算机系统,用于控制和监测各种操作参数-温度,pH值,沼气成分等。用标准方法和气相色谱法测定总固体、总有机物和挥发性脂肪酸。分析了日沼气产量及其主要成分(CH4、CO2、H2S)。在厌氧消化过程中,分离出了芽孢杆菌属、假单胞菌属、肠球菌属、气单胞菌属以及嗜盐杆菌属等不同种类的微生物。具有已知致病性的有假单胞菌、肠球菌和气单胞菌。对所有分离菌株的耐药性研究显示,它们对氨苄西林、阿莫西林、杆菌肽、头孢曲松、庆大霉素和万古霉素具有耐药性。根据已证实的耐药机制资料,详细讨论了部分菌株的纤维素降解活性、致病潜力和耐药性。
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引用次数: 1
REMOVAL OF HEAVY METAL Cr (II), Ni (II), Cu (II), Zn (II), Cd (II), Pb (II) IONS FROM AQUEOUS SOLUTION BY MENTHA PIPERITA EXTRACT 薄荷提取物去除水溶液中重金属Cr (II)、Ni (II)、Cu (II)、Zn (II)、Cd (II)、Pb (II)离子
Pub Date : 2020-10-15 DOI: 10.32006/eeep.2020.2.1520
E. Yucel, M. Yucel
In this study, the usage of the peppermint (Mentha piperita) for extracting the metal ions [Mg (II), Cr (II), Ni (II), Cu (II), Zn (II), Cd (II), Pb (II)] that exist at water was investigated. In order to analyze the stability properties, Langmuir, Freundlich, Temkin and Dubinin-Radushkevich isotherms were used at removing the metal ions and the highest correlation coefficients (R2) were obtained at Langmuir isotherm. Therefore, it is seen that the Langmuir model is more proper than the Freundlich model. However, it was found that the correlation coefficients of removing Ni and Cd is higher at Freundlich model than Langmuir and low at Dubinin-Radushkevich isotherm. It is established that the biosorption amount increase depends on the increase of biosorbent and it can be achieved high efficiency (95%) even with small amount (0.6 mg, peppermint extract) at lead ions. It is also determined that the peppermint extracted that is used at this study shows high biosorption capacity for metal ions and can be used for immobilization of metals from polluted areas.
本文研究了薄荷提取水中存在的金属离子[Mg (II)、Cr (II)、Ni (II)、Cu (II)、Zn (II)、Cd (II)、Pb (II)]。为了分析其稳定性,采用Langmuir、Freundlich、Temkin和Dubinin-Radushkevich等温线来去除金属离子,在Langmuir等温线处获得了最高的相关系数(R2)。由此可见,Langmuir模型比Freundlich模型更为合适。但在Freundlich模型下去除Ni和Cd的相关系数比Langmuir模型高,而在Dubinin-Radushkevich等温线上去除Ni和Cd的相关系数较低。结果表明,生物吸附剂用量的增加对生物吸附剂的吸附量有较大的影响,在铅离子条件下,即使添加少量(0.6 mg,薄荷提取物)生物吸附剂也能达到较高的吸附效率(95%)。研究还表明,本研究中使用的薄荷提取物对金属离子具有较高的生物吸附能力,可用于固定污染地区的金属。
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引用次数: 0
INVESTIGATION OF NATURAL RADIOACTIVITY OF MINERAL WATERS IN SOUTHERN BULGARIA 保加利亚南部矿泉水天然放射性调查
Pub Date : 2020-10-15 DOI: 10.32006/eeep.2020.2.2127
E. Geleva, H. Protohristov, N. Goutev, Vladimir Bashev, S. Genchev, D. Tonev
The activity of naturally occurring radionuclides in mineral waters from certain most frequently used sources in Rhodope Mountains region, Southern Bulgaria has been measured with high precision by means of nuclear and radiochemical methods. The survey is pointed at radium-226 (226Ra), lead-210 (210Pb) and natural uranium (nat. U), which are most important from the point of view of public health in Bulgaria, because of their high toxicity and radiotoxicity in drinking water. Seventeen water sources were under investigation. The activity concentrations of 226Ra and 210Pb varied from 13 to168 mBq/L and from ≤ 1.8 to 104 mBq/L respectively. The concentration of nat. U in the mineral water was in the range ≤ 3 to 21 µg/L.The annual effective doses were calculated for all investigated waters for adult inhabitants assuming yearly consumption of 730 litres.The results have shown that all values of the annual effective dose of ingestion of mineral waters were below the individual dose criterion of 100 µSv/y reported by World Health Organization (WHO).The obtained new results are used to assess the radiation status of the investigated waters. They will support timely and adequate measures to reduce the harmful impact of ionizing radiation on the population in cases of increased radioactivity
利用核化学和放射化学方法高精度地测量了保加利亚南部罗多佩山区某些最常用来源的矿泉水中天然存在的放射性核素的活度。调查针对的是镭-226 (226Ra)、铅-210 (210Pb)和天然铀(天然铀),从保加利亚公共卫生的角度来看,这三种物质最为重要,因为它们在饮用水中具有高毒性和放射性毒性。17个水源正在调查中。226Ra和210Pb的活性浓度分别在13 ~ 168 mBq/L和≤1.8 ~ 104 mBq/L之间变化。矿泉水中硝酸铀的浓度范围为≤3 ~ 21µg/L。假定成年居民年消费量为730升,计算了所有调查水域的年有效剂量。结果表明,所有矿泉水年有效摄入剂量值均低于世界卫生组织(WHO)规定的100µSv/y的个体剂量标准。所得新结果用于评价调查水域的辐射状况。它们将支持及时和适当的措施,以便在放射性增加的情况下减少电离辐射对人口的有害影响
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引用次数: 0
ECOLOGICAL-PHYTOGEOGRAPHIC CHARACTERISTICS OF THE MAIN EDIFICATORY SPECIES IN THE FORESTS OF LOZENSKA MOUNTAIN 罗赞斯卡山森林主要熏蒸物种的生态植物地理特征
Pub Date : 2020-10-15 DOI: 10.32006/eeep.2020.2.4551
Plamen Glogov, M. Georgieva
The study covers Phase 2 of the ecological-phytogeographic analysis of the dendroflora of Lozenska Mountain. Its purpose is to characterize 15 species-edificators of tree and shrub communities in the mountain and to point out the indicators that most determine the assessment of their ability to long-term adaptability to mountain conditions (convertibility). The performed characterization of the edificators with respect to the reference species shows some heterogeneity in the influence of the factors and the distribution of the groups of species. Indicators, which determinate the most convertibility assessments of edificators are cryoclimatic factor, edaphic regimes and soil pH.
本研究为洛赞斯卡山树木区系生态植物地理分析的第二阶段。其目的是描述山区树木和灌木群落的15种培育物种的特征,并指出最能决定评估它们对山区条件的长期适应能力(可转换性)的指标。对参考物种的影响因子和类群的分布进行了表征,显示出一定的异质性。决定促生剂最易转换性评价的指标是低温气候因子、土壤状况和土壤pH值。
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引用次数: 1
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Ecological Engineering and Environment Protection
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