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MICROBIOLOGICAL STUDY OF THE ANTHROPOGENIC PRESSURE ON THE YANTRA RIVER IN THE REGION OF VELIKO TARNOVO 大特尔诺沃地区延特拉河人为压力的微生物学研究
Pub Date : 2022-11-20 DOI: 10.32006/eeep.2022.2.1829
Z. Tsvetanova, Dimitar Dimitrov, H. Naidenski
The report discusses the microbiological status of the Yantra River in the region of the town of Veliko Tarnovo. Water samples collected at 11 points of the river stretch were analyzed for quantification of E. coli and coliforms, intestinal enterococci and heterotrophic bacteria. It was found increased levels of fecal indicator bacteria in the river waters, varying over time and along the riverside. The number of viable E. coli and enterococci increased under the influence of untreated wastewater influx and the Belitsa River.
该报告讨论了大利科特尔诺沃镇地区延特拉河的微生物状况。对河段11个点的水样进行了分析,定量分析了大肠杆菌和大肠菌群、肠球菌和异养细菌。研究发现,河水中粪便指示细菌的含量有所增加,随时间和沿河而变化。在未经处理的废水流入和Belitsa河的影响下,活大肠杆菌和肠球菌的数量增加。
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引用次数: 0
A THEORETICAL OVERVIEW OF COMPRESSED AIR ENERGY STORAGE TECHNOLOGIES AND DEVELOPMENTS 压缩空气储能技术及其发展的理论概述
Pub Date : 2022-11-20 DOI: 10.32006/eeep.2022.2.3044
Leila Bakhtiari, D. Karamanev
In our modern world, technological developments on the one hand, and global warming and its consequences, on the other hand, cause us to feel the necessity for reliable, cost-effective, and clean energy. Using renewable energy sources paired with compressed air energy storage can be a good option that meets these expected criteria. Although a compressed air energy storage system (CAES) is clean and relatively cost-effective with long service life, the currently operating plants are still struggling with their low round trip efficiencies. This paper illustrates an up-to-date review of compressed air energy storage systems containing changes in the conventional process to improve performance and increase efficiency. Three main categories of compressed air energy storage technology, diabatic, adiabatic, and isothermal, are analyzed theoretically. In addition, three components of a compressed air energy storage system including compression system, reservoirs, and expansion system are discussed here in detail. The advantages, disadvantages, and the technological readiness of different types of CAES are discussed.
在我们的现代世界,一方面是技术的发展,另一方面是全球变暖及其后果,使我们感到需要可靠、经济、清洁的能源。使用可再生能源与压缩空气储能相结合是满足这些预期标准的一个很好的选择。尽管压缩空气储能系统(CAES)清洁,使用寿命长,成本效益相对较低,但目前运行的工厂仍在努力解决其低往返效率的问题。本文说明了压缩空气储能系统的最新审查包含在常规过程的变化,以提高性能和提高效率。从理论上分析了压缩空气储能技术的三大类:绝热、绝热和等温。此外,还详细讨论了压缩空气储能系统的三个组成部分:压缩系统、储气罐和膨胀系统。讨论了不同类型CAES的优点、缺点和技术成熟度。
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引用次数: 0
ASSESSMENT OF THE EXPECTED INDUCED RADIOACTIVITY IN THE BUNKER WALLS AT THE INRNE-BAS CYCLOTRON FACILITY 内巴斯回旋加速器设施掩体壁预期诱发放射性的评估
Pub Date : 2022-11-20 DOI: 10.32006/eeep.2022.2.7378
A. Demerdjiev, D. Tonev, N. Goutev, D. Dimitrov, G. Dimitrova, E. Geleva, S. Genchev
The Institute for Nuclear Research and Nuclear Energy at the Bulgarian Academy of Sciences is working on the construction of a cyclotron centre. Оne of the challenges in designing the radiation protection of a cyclotron centre is the preliminary assessment of the expected amounts of radioactive waste that will be generated during the lifetime of the facility. Our aim in this paper is to make an estimation of the expected induced radioactivity in the cyclotron bunker walls after the 20 year operational period of the facility. For this purpose, Monte Carlo simulations with the code FLUKA were performed. The distributions of the specific activity of the generated radionuclides in the concrete walls and of the residual dose rates inside the bunker are obtained. The specific activity of the most important long-lived radionuclides in the concrete walls of the bunker, namely 154Eu, 152Eu, 134Cs, 60Co, 54Mn is estimated. On the basis of these results an optimization of the shielding design is proposed in order to decrease the amount of radioactive waste which will be generated during the facility lifetime.
保加利亚科学院的核研究和核能研究所正在建造一个回旋加速器中心。Оne设计回旋加速器中心辐射防护的挑战之一是对该设施在使用寿命期间将产生的放射性废物的预期数量进行初步评估。本文的目的是对该设施运行20年后回旋加速器掩体壁的预期诱导放射性进行估计。为此,使用FLUKA代码进行蒙特卡罗模拟。得到了所产生的放射性核素在混凝土壁上的比活度分布和掩体内的残留剂量率分布。对碉堡混凝土壁上最重要的长寿命放射性核素154Eu、152Eu、134Cs、60Co、54Mn的比活度进行了估算。在这些结果的基础上,提出了屏蔽设计的优化,以减少在设施寿命期间产生的放射性废物的数量。
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引用次数: 0
BACTERIAL DEGRADATION OF CELLULOSIC SUBSTRATES UNDER TERRESTRIAL AND LONG TERM MANNED SPACE MISSION CONDITIONS: A REVIEW (Part I) 在陆地和长期载人航天任务条件下细菌降解纤维素基质:综述(第一部分)
Pub Date : 2022-11-20 DOI: 10.32006/eeep.2022.2.6067
L. Dimitrova, V. Hubenov, L. Kabaivanova, Y. Gocheva, P. Angelov, H. Najdenski
The Earth and the lower atmosphere (troposphere and stratosphere) are constantly faced with numerous environmental challenges, one of which is the growing pollution due to the incineration of cellulose-containing waste with accumulating potential. In recent years scientists have focused on the complexity of ecological mechanisms in the biosphere of our planet - Earth, starting from laboratory, scaled and closed ecosystems. Onboard the spacecraft, textile products with antimicrobial properties are widely used which limits the spread of infections and ensures safety, comfort and resistance of the user. Another type of waste is the remains of sanitary and medical consumables, personal hygiene materials (e.g. wet and dry wipes, toilet paper, etc.), paper, inedible parts of greenhouse plants, etc., being usually subjected to microbial degradation. On Earth, the accumulation of these cellulose containing waste can cause serious environmental problems. Nowadays, many researchers are trying in experimental conditions on Earth to solve the problem of cellulose-containing waste by means of different approaches – burning, composting, burial, etc. The main risk and environmental problem is that the burial of waste in the soil and composting should contribute to the spread of microorganisms with pathogenic potential. Nevertheless, a promising approach is the microbial degradation of cellulose containing substrates realized by microbial consortia depending on the conditions of the surrounding environment. Therefore, the recent review aims to make a comparative analysis of the bacterial species involved in the degradation processes of cellulose-containing waste and to assess their potential for possible application in space conditions, including the International Space Station.
地球和低层大气(对流层和平流层)不断面临着许多环境挑战,其中之一是由于焚烧含有纤维素的废物而产生的日益严重的污染。近年来,科学家们从实验室、规模和封闭的生态系统开始,关注我们星球——地球生物圈中生态机制的复杂性。在航天器上,具有抗菌性能的纺织品被广泛使用,这限制了感染的传播,并确保了用户的安全性、舒适性和抵抗力。另一类废物是卫生和医疗消耗品、个人卫生材料(如湿巾和干纸巾、厕纸等)、纸张、温室植物的不可食用部分等的残留物,通常会受到微生物的降解。在地球上,这些含有纤维素的废物的积累会导致严重的环境问题。目前,许多研究人员正在地球上的实验条件下尝试通过燃烧、堆肥、掩埋等不同的方法来解决含纤维素废物的问题。主要的风险和环境问题是,将废物埋在土壤和堆肥中会导致具有致病潜力的微生物的传播。然而,一种很有前途的方法是微生物降解含有底物的纤维素,这取决于周围环境的条件,由微生物联合体实现。因此,最近的审查旨在对参与含纤维素废物降解过程的细菌种类进行比较分析,并评估它们在包括国际空间站在内的空间条件下可能应用的潜力。
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引用次数: 0
METHODOLOGY FOR REMOTE SENSING MONITORING OF ORGANIC WHEAT CROPS 有机小麦作物遥感监测方法学
Pub Date : 2022-11-20 DOI: 10.32006/2022.2.5659
Milen Chanev, Lachezar Hristov Filchev, D. Valcheva
In the presented methodology for aerospace monitoring of autumn wheat crops, grown under the conditions of organic farming, the ways of applying ground and aerospace methods are discussed in detail. This includes field experiments, phenological observations, GIS and remote sensing methods and data (data from Sentinel-2 satellite and WingtraOne unmanned aerial vehicle with MicaSense RedEdge-MX multispectral camera and RGB camera) and statistical analyses. In order to achieve the aim and objectives of the study, an experiment was conducted on a organically certified production field sown with einkorn (Triticum monococum) in the period 2020-2021. The field is part of the holding of ET "Borislav Slavchev" in the village of Byala Reka, Parvomai Municipality, South-Central Bulgaria on the soil type of leached chernozem clays, with a size of 136 da.
在本文提出的有机栽培条件下秋小麦作物航空监测方法中,详细讨论了地面和航空监测方法的应用方法。这包括实地实验、物候观测、GIS和遥感方法和数据(来自Sentinel-2卫星和搭载MicaSense RedEdge-MX多光谱相机和RGB相机的WingtraOne无人机的数据)以及统计分析。为了实现研究的目的和目标,在2020-2021年期间,在一个有机认证的生产田里进行了一项试验,该试验种植了einkorn (Triticum monococum)。这块田是ET“Borislav Slavchev”在保加利亚中南部Parvomai市Byala Reka村持有的土地的一部分,土壤类型为浸出黑钙土粘土,面积为136公顷。
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引用次数: 1
HOW WHITE BIOTECHNOLOGY CAN CONTRIBUTE TO BIOECONOMY? 白色生物技术如何促进生物经济?
Pub Date : 2022-11-20 DOI: 10.32006/eeep.2022.2.0517
J. François
Biotechnology in its broadest sense is the application of science and technology to living organisms to produce goods, products and services. It is segmented into 10 branches, of which white biotechnology, also called industrial biotechnology, is the oldest, having its roots in the Neolithic period with the transformation of food into fermented products attributed to divine intervention at that time. White biotechnology really took off after Louis Pasteur demonstrated that fermentation is caused by living organisms. This discovery was followed in the early 20th century by the development of large-scale fermentation processes exploiting the intrinsic metabolic properties of microorganisms, such as solventogenesis in Clostridium or secondary metabolism for penicillin synthesis in Penicillium. With the advent of recombinant DNA in the 1970s, a new era of white biotechnology was born, with the ability to genetically manipulate microorganisms for the production of recombinant proteins and therapeutic agents, which notably boosted the biopharmaceutical sector. A third revolution in white biotechnology is nowadays occurring, driven by the strong societal demand to shift from a fossil fuel-based economy to one based on renewable carbon resources. The transition to so-called "bioeconomy" is expected to be slow and painful because it relies on the exploitation of "biological systems" that, unlike the chemical processes, are much more complex, inefficient, difficult to manage and still unpredictable. After a brief history of industrial biotechnology, I will present and address in this opinion paper some major challenges that await white biotechnology, using as an example our current work in the production of biosourced methionine, and I will discuss societal factors that could foster a bright future to white biotechnology in our modern society.
从最广泛的意义上讲,生物技术是将科学技术应用于生物体以生产商品、产品和服务。它分为10个分支,其中最古老的是白色生物技术,也被称为工业生物技术,它起源于新石器时代,当时的上帝干预将食物转化为发酵产品。白色生物技术真正起飞是在路易斯·巴斯德证明了发酵是由生物体引起的之后。这一发现之后,在20世纪早期,大规模发酵工艺的发展利用了微生物固有的代谢特性,如梭状芽胞杆菌的溶剂生成或青霉菌合成青霉素的次级代谢。随着20世纪70年代重组DNA的出现,白色生物技术的新时代诞生了,人们能够通过基因操纵微生物来生产重组蛋白质和治疗剂,这极大地推动了生物制药行业的发展。在强烈的社会需求推动下,从以化石燃料为基础的经济转向以可再生碳资源为基础的经济,白色生物技术的第三次革命正在发生。向所谓的“生物经济”的过渡预计将是缓慢而痛苦的,因为它依赖于对“生物系统”的开发,而与化学过程不同,“生物系统”要复杂得多,效率低下,难以管理,而且仍然不可预测。在简要介绍了工业生物技术的历史之后,我将在这篇意见论文中介绍并解决白色生物技术面临的一些主要挑战,以我们目前在生产生物源蛋氨酸方面的工作为例,我将讨论在我们现代社会中可以促进白色生物技术光明未来的社会因素。
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引用次数: 0
DISTRIBUTION OF TRITIUM IN SURFACE WATER AND PRECIPITATION IN RILA MOUNTAIN, BULGARIA 保加利亚里拉山地表水和降水中氚的分布
Pub Date : 2022-04-30 DOI: 10.32006/eeep.2022.1.7076
E. Geleva, D. Tonev, H. Protohristov, Svetoslav Dimitrov, Vladimir Bashev, S. Genchev, A. Demerdjiev
A study of the tritium content of surface water from three Rila lakes – Moussala lake, Aleko lake and Ice lake, and precipitation at Moussala mount has been performed by means of high precision nuclear and radiochemical methods. The measurements are part of a long-term environmental monitoring program developed and maintained by the Institute for Nuclear Research and Nuclear Energy, Bulgaria Academy of Science. The results for lake water and precipitation samples collected at Moussala part of Rila over the period 2012–2017 were found to range from ˂ 1.20 to 2.77 Bq.L-1 and from 2.14 to 4.12 Bq.L-1 respectively. The content of 3H in all samples is below the recommended level of 100 Bq.L-1.The obtained new results are used to assess the radiation status of the investigated water sources and can be used for evaluation of possible future changes and trends.
用高精度的核化学和放射化学方法研究了穆萨拉湖、阿列科湖和冰湖三个湖泊的地表水和穆萨拉山降水的氚含量。这些测量是保加利亚科学院核研究和核能研究所制定和维护的长期环境监测计划的一部分。在2012-2017年期间,在里拉的穆萨拉地区收集的湖水和降水样本的结果发现,其范围为小于1.20至2.77 Bq。L-1和2.14 ~ 4.12 Bq。l - 1。所有样品中3H的含量均低于100bq . l -1的推荐水平。所获得的新结果可用于评价所调查水源的辐射状况,并可用于评价未来可能的变化和趋势。
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引用次数: 0
EXTREMUM SEEKING BASED COMPOSED RECURSIVE MODEL FREE CONTROL OF TWO-STAGE ANAEROBIC DIGESTION PROCESS 基于极值搜索的两段厌氧消化过程组合递归模型自由控制
Pub Date : 2022-04-30 DOI: 10.32006/eeep.2022.1.4555
Ning Pan, Haoping Wang, Yang Tian, N. Christov, I. Simeonov
In this paper, a new structure of extremum seeking algorithm is applied to the two-stage anaerobic digestion process to maximize the outflow rate of both hydrogen and methane. The model of the two-stage AD process is presented, which provides the characteristics of the total gas production rate. Based on the original Extremum Seeking Control (ESC), a novel Composed Recursive Model Free Controller (CRMFC) is added for maximum tracking for the gas production in the bioreactors. The proposed controller comprises a recursive model free stabilization term and a recursive time delay compensation term. Standard ESC, Newton-based ESC and Kalman filter (KF) based ESC are respectively combined with the new model-free controller to verify the proposed structure. Numerical simulations illustrate the performance of the proposed controller.
本文将一种新的极值搜索算法结构应用于两段厌氧消化过程中,以最大限度地提高氢气和甲烷的流出率。建立了两阶段AD过程模型,给出了总产气量的特征。在原有的极值寻求控制(ESC)的基础上,增加了一种新的组合递归模型无控制器(CRMFC),用于生物反应器产气量的最大跟踪。该控制器包括一个递归无模型镇定项和一个递归时滞补偿项。将标准ESC、基于牛顿的ESC和基于卡尔曼滤波(KF)的ESC分别与新的无模型控制器相结合,验证了所提出的结构。数值仿真验证了所提控制器的性能。
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引用次数: 1
CHARACTERISTICS OF METASEQUOIA GLYPTOSTROBOIDES ARTIFICIAL COMMUNITY КОKАLУАNЕ VILLAGE, SOFIA REGION 索非亚地区水杉人工群落特征КОkАlУАnЕ村
Pub Date : 2022-04-30 DOI: 10.32006/eeep.2022.1.5661
Plamen Glogov, G. Zaemdzhikova
The aim of the present study is phytocoenological characteristics and preliminary assessment of the health status of the first Dawn redwood (Metasequoia glyptostroboides) artificial plantation in Bulgaria established in 1969. The plantation of metasequoia is located in the land of the Kokalyane village (Sofia region) on an area of about 2500 m2. The study was conducted in the period April-July, 2021. The results show that the artificial stand was created on the locality of indigenous communities of Alnus glutinosa, which occupy the main part of the Iskar river banks between Plana and Lozenska mountains. The health status of the Dawn redwood plantation was assessed as “good”. From the distance of the 50-year period, the artificial afforestation with this exotic relic in the area of the village of Kokalyane can be considered successful.
本文对保加利亚1969年建立的第一个水杉人工林的植物群落学特征和健康状况进行了初步评价。水杉种植园位于Kokalyane村(索菲亚地区)的土地上,面积约2500平方米。该研究于2021年4月至7月期间进行。结果表明,人工林分是在位于Plana和Lozenska山脉之间的Iskar河岸主体地带的Alnus glutinosa土著群落上建立的。黎明红木人工林健康状况评价为“良好”。从50年的时间来看,在Kokalyane村地区用这种异国文物进行的人工造林可以被认为是成功的。
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引用次数: 0
PIG FARMS AND THEIR SURROUNDINGS AS A FACTOR IN THE SPREAD OF ANTIMICROBIAL RESISTANCE 猪场及其周围环境是抗菌素耐药性传播的一个因素
Pub Date : 2022-04-20 DOI: 10.32006/eeep.2022.1.1421
L. Dimitrova, M. Zaharieva, H. Najdenski
In recent decades, in modern intensive animal husbandry, there has been a worrying trend towards the selection and spread of bacterial strains resistant to antibiotics. This global phenomenon has not only a certain negative effect on therapeutic practice, but also poses a serious risk to the spread of resistance in the food chain and in humans. Various genera from the family Enterobacteria like Salmonella, Shigella, Klebsiella, Yersinia, Citrobacter, etc. and especially Escherichia coli have a special place in this process. The most commonly used antibiotics for treatment of patients with complicated Salmonella infections are ceftriaxone and ciprofloxacin, to which the bacteria has already developed antibiotic resistance. For Escherichia coli, significantly increased resistance is developed to the third-generation cephalosporins, aminoglycosides, as well as combined resistance to three key antimicrobial groups (fluoroquinolones, third-generation cephalosporins and aminoglycosides). Resistance among Yersinia spp. against tetracyclines and fluorochinolones has been reported to the scientific community in numerous studies on human and animal isolates. By using the raw manure as fertilizer, these microorganisms may be transmitted to soil, wastewater or others environmental niches. Further, they can be transmitted via the food chain to humans and animals. Considering that the horizontal gene transfer is one of the most important mechanisms for transmitting of antimicrobial resistance, then after consumption of contaminated food, water, etc., genetically determined antibiotic resistance in animals can easily affect resistance among people, which makes vital drugs ineffective against serious illnesses. For the possible spread of antimicrobial resistance in pigs and related ecological niches - pig farms, manure lagoons, wastewater and soils, large-scale studies are being conducted to clarify their ecological and health significance. In addition, the results obtained will contribute to the development of an effective national strategy for the control of antimicrobial resistance.
近几十年来,在现代集约化畜牧业中,出现了一种令人担忧的趋势,即抗生素耐药菌株的选择和传播。这一全球现象不仅对治疗实践产生一定的负面影响,而且对耐药性在食物链和人类中传播构成严重风险。沙门氏菌、志贺氏菌、克雷伯氏菌、耶尔森氏菌、柠檬酸杆菌等肠杆菌科的各个属,尤其是大肠杆菌在这一过程中占有特殊的地位。治疗复杂沙门氏菌感染患者最常用的抗生素是头孢曲松和环丙沙星,细菌已经对这两种抗生素产生了耐药性。大肠杆菌对第三代头孢菌素、氨基糖苷类药物的耐药性显著增强,对三种主要抗菌素类药物(氟喹诺酮类药物、第三代头孢菌素和氨基糖苷类药物)的联合耐药性也显著增强。在对人类和动物分离株的大量研究中,已经向科学界报告了耶尔森氏菌对四环素和氟喹诺酮类药物的耐药性。通过使用生粪肥作为肥料,这些微生物可能会传播到土壤、废水或其他环境生态位。此外,它们可以通过食物链传播给人类和动物。考虑到水平基因转移是抗菌素耐药性传播的最重要机制之一,那么在食用被污染的食物、水等后,动物基因决定的抗生素耐药性很容易影响人类的耐药性,这使得重要的药物对严重疾病无效。对于抗菌素耐药性可能在猪和相关生态位(猪场、粪池、废水和土壤)中传播,正在进行大规模研究,以阐明其生态和健康意义。此外,获得的结果将有助于制定有效的国家战略,控制抗菌素耐药性。
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引用次数: 0
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Ecological Engineering and Environment Protection
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