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Economics of Inter-Cropping: A Case Study of Onion and Tomato at District Muzaffargarh, Punjab, Pakistan 套种经济:以巴基斯坦旁遮普邦穆扎法尔格尔地区洋葱和番茄为例
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-04 DOI: 10.53560/ppasb(60-3)833
Muhammad Nisar Khan, Gulnaz Hameed, Arshad Mahmood Malik, Saima Asad
The current research is being conducted in Muzaffargarh district of Punjab, Pakistan to investigate the profitability and land equivalent ratio of intercropping onion and tomato. An economic analysis of intercropping in the Muzaffargarh district can inform farmers about the profitability and sustainability of this practice, aiding their decision-making between intercropping and monocropping. The study utilized a simple random sampling technique to select 45 vegetable growers out of 60, from two major vegetable-growing villages; Hajiwah and Beli Janubi. Descriptive analysis, including frequency distribution, mean, and percentages, was used to analyze the data. The results of the study showed that intercropping had a significantly higher yield (17897 kg/acre) than sole cropping of onions (6075 kg/acre) and tomatoes (16050 kg/acre). Intercropping also had a higher benefit-cost ratio of 1.59, compared to onion sole cropping (1.37) and tomato sole cropping (1.48). The land equivalent ratio was 1.31, which indicated that intercropping was more efficient in terms of land use than sole cropping. The study also revealed that intercropping onions and tomatoes provided additional income to farmers and helped maximize land use. However, farmers encountered challenges such as high seed costs, diseases, low output prices, and high transportation costs. In conclusion, the study suggested that intercropping onion and tomato is a viable agronomic strategy in the Muzaffargarh district, as it improves land-use efficiency and maximizes returns. The study showed that intercropping complemented each other and contributed to increasing yield per unit area and improving nutritional properties.
本研究在巴基斯坦旁遮普邦的Muzaffargarh地区进行,旨在调查洋葱和番茄间作的盈利能力和土地当量比。对Muzaffargarh地区间作的经济分析可以让农民了解这种做法的盈利能力和可持续性,帮助他们在间作和单作之间做出决策。该研究采用简单的随机抽样技术,从两个主要蔬菜种植村的60名蔬菜种植者中选择了45名;Hajiwah和Beli Janubi。描述性分析,包括频率分布、平均值和百分比,用于分析数据。研究结果表明,间作的产量(17897公斤/英亩)明显高于单独种植洋葱(6075公斤/亩)和番茄(16050公斤/英亩。与洋葱单作(1.37)和番茄单作(1.48)相比,间作的效益成本比也更高,为1.59。土地当量比为1.31,这表明间作在土地利用方面比单作更有效。研究还表明,洋葱和番茄间作为农民提供了额外的收入,并有助于最大限度地利用土地。然而,农民遇到了诸如种子成本高、疾病、产出价格低和运输成本高等挑战。总之,该研究表明,在穆扎法尔格尔地区,洋葱和番茄间作是一种可行的农艺策略,因为它可以提高土地利用效率并最大限度地提高回报。研究表明,间作相互补充,有助于提高单位面积产量和改善营养特性。
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引用次数: 0
Role of Dof Transcription Factors under Abiotic Stresses Dof转录因子在非生物胁迫中的作用
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-04 DOI: 10.53560/ppasb(60-3)905
Amjid Khan, Fouzia Bibi, Sohail Ahmad Jan, Zabta Khan Shinwari
For the survival of the rapidly growing global populace, plant species must exhibit tolerance towards climate change. Plants possess mechanisms to respond to stress by changing their biological processes and stimulating stress-responsive genes. The Dof (TFs) family, which binds to DNA with a single finger, reflects a plant-specific group of TFs that play an important part in regulating plants that are facing different types of abiotic stresses which may influence their growth and development. Discovery of this family has made a significant impact on the field of plant sciences. However, the characterization of Dof transcription factors in crop plants is currently limited reported. Several Dof transcription factors (TFs) of plants have been shown in nature. The transcription factors TaDofs, StDof, MnDofs, JrDof3TF, Va/VvDofs, GhDof1, OsDof1, SmeDof, ZmDof, CsDof, DcDof, CaDofs, ThDof, BraDof, and AcDof are important for abiotic stressors such heat, cold, salt, drought, and heavy metals. In addition, Dof transcription factors play a role in the regulation of factors related to yield and quality. Nevertheless, some outstanding issues remain. The review article provides a summary of the role of various stress-responsive Dof transcription factors in response to abiotic stresses. Additionally, this study investigates the limitations and possible opportunities associated with Dof transcription factors in the development of crops that are capable of withstanding climate change. Therefore, it is recommended to conduct comprehensive research on Dof transcription factors (TFs) across many different transcription factors fields to find their potential novel functionality, which will be beneficial to our retention of the delicate biological processes in plants.
为了快速增长的全球人口的生存,植物物种必须对气候变化表现出耐受性。植物通过改变自身的生理过程和刺激应激反应基因来应对逆境。Dof (TFs)家族,通过单指结合DNA,反映了一组植物特异性的TFs,在调节植物面临不同类型的非生物胁迫时发挥重要作用,这些胁迫可能影响其生长和发育。这一科的发现对植物科学领域产生了重大影响。然而,目前关于Dof转录因子在作物中的表征报道有限。植物的多种转录因子(TFs)已在自然界中被发现。转录因子TaDofs、StDof、MnDofs、JrDof3TF、Va/VvDofs、GhDof1、OsDof1、SmeDof、ZmDof、CsDof、DcDof、CaDofs、ThDof、BraDof和AcDof对热、冷、盐、干旱和重金属等非生物胁迫具有重要作用。此外,Dof转录因子对产量和品质相关因子也有调控作用。然而,仍然存在一些悬而未决的问题。本文综述了各种应激响应型Dof转录因子在非生物胁迫下的作用。此外,本研究还探讨了Dof转录因子在能够抵御气候变化的作物发育中的局限性和可能的机会。因此,建议对不同转录因子领域的Dof转录因子(TFs)进行综合研究,以发现其潜在的新功能,这将有利于我们保留植物中微妙的生物过程。
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引用次数: 0
Amending Soil with Rhizobium carrying Biochar Ameliorates Drought Stress on Phaseolus vulgaris 携带生物炭的根瘤菌改良土壤改善菜豆的干旱胁迫
Q4 Agricultural and Biological Sciences Pub Date : 2023-08-30 DOI: 10.53560/ppasb(60-3)882
Aniqa Batool, A. Rashid, I. Aziz
As a consequence of climate change/global warming earth’s agriculture output is under rigorous stress. There is a growing need to develop strategies to cope with these abiotic stresses. Biochar exhibiting many beneficial qualities appeared to alleviate these problems by improving soil fertility by adding carbon and preventing nutrient losses etc. Biochar can also enhance BNF and could be used as a carrier for rhizobium by providing a suitable microenvironment. The current study is aimed to find the ameliorative potential of different biochar types to be used as rhizobium carriers for Phaseolus vulgaris L. exposed to drought stress. Both types of biochar were analyzed for physico-chemical and morphological parameters. Presence of Silicon content remains the key finding for rice husk biochar which was absent in Lantana biochar. Increased C, K, and Ca weight percentages were found in Lantana biochar as compared to their proportions for rice husk biochar. On the contrary, the oxygen content was higher in rice husk biochar as compared to that in Lantana. Phaseolus seeds were used for the pot experiment where stress treatment was applied by FTSW (Fractionable Transpirable Soil Water) technique. One isolated strain along with two types of biochar carrier was applied to the plants in combination with water stress treatment. Plants were analyzed for growth and physiological parameters including plant height, leaf area, biomass, photosynthesis, transpiration rate, stomatal conductance, and water use efficiency, where rice husk biochar responded better than the one obtained from Lantana. Plants responded positively for all the growth as well as physiological parameters when treated in combination with the inoculum for both stress levels i.e., 100% and 60% field capacity F.C. The present study advocates rice husk biochar for its ability to enhance tolerance in Phaseolus against drought stress through its role as an inoculum carrier contributing suitable habitat for the microorganism.
由于气候变化/全球变暖,地球的农业产量正处于严峻的压力之下。人们越来越需要制定应对这些非生物压力的策略。生物炭表现出许多有益的特性,通过增加碳和防止养分流失等改善土壤肥力,缓解了这些问题。生物炭还可以提高生物固结生物量,并通过提供适宜的微环境作为根瘤菌的载体。本研究旨在探索不同类型生物炭作为干旱胁迫下菜豆根瘤菌载体的改良潜力。对两种类型的生物炭进行了理化和形态参数分析。稻壳生物炭中硅含量的存在是稻壳生物炭的关键发现,而稻壳生物炭中硅含量的存在是不存在的。与稻壳生物炭相比,蓝貂生物炭中C、K和Ca的重量百分比有所增加。相反,稻壳生物炭中的氧含量高于大灯笼。以菜豆种子为试验材料,采用FTSW (fractional Transpirable Soil Water)技术进行胁迫处理。将1株分离菌株和2种生物炭载体与水分胁迫处理相结合施用于植株。对植物的生长和生理参数(株高、叶面积、生物量、光合作用、蒸腾速率、气孔导度和水分利用效率)进行了分析,结果表明稻壳生物炭的反应优于大灯笼。在100%和60%田间容量F.C.的胁迫水平下,稻壳生物炭对所有生长和生理参数均有积极的响应。本研究认为,稻壳生物炭作为一种接种载体,为微生物提供适宜的栖息地,从而提高了Phaseolus对干旱胁迫的耐受性。
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引用次数: 0
An Estimate of Protective Immunity against SARS-CoV2: Comparison of Different Vaccine Types 对严重急性呼吸系统综合征冠状病毒2型保护性免疫的评估:不同疫苗类型的比较
Q4 Agricultural and Biological Sciences Pub Date : 2023-08-24 DOI: 10.53560/ppasb(60-3)749
Waseem Iqbal, Ikram Ullah, Uroosa Irum, Saima Siddiqi, Khalid Mahmood Khan
Several types of vaccines have been approved to prevent SARS-CoV-2 infection. Few studies are conducted on the efficacy of COVID-19 vaccines. Vaccination is important to eliminate and fight SARS-CoV-2 infection and several vaccines have been approved. This study aimed to assess the incidence density of COVID-19 infection among the community, estimate the effectiveness of different types of vaccines (inactivated virus, viral vector or mRNA) and efficiency of incomplete and complete vaccination. In this observational cross-sectional study, a total of 4924 specimens were received from 1st January 2022 to 2nd February 2022 for the detection of SARS-CoV-2. The patient’s age, gender, and vaccination data were recorded and S, N, and ORF 1ab genes were amplified after RNA extraction through PCR. out of which 1034 (20.99%) cases were positive. Among 1034 (20.99%) positive cases, 418 and 616 patients were vaccinated and non-vaccinated respectively. The cases of SARS-CoV-2 in vaccinated patients were categorized into a sudden infection (≤ 10 days) and late infection (≥10 days) after the incomplete and complete dose of vaccination. Vaccination provides partial protection against SARS-CoV-2 infection. This might be due to the low efficacy and inability to detect recent variations in the protein structure of the virus.
几种类型的疫苗已被批准用于预防严重急性呼吸系统综合征冠状病毒2型感染。很少对新冠肺炎疫苗的效力进行研究。疫苗接种对消除和对抗严重急性呼吸系统综合征冠状病毒2型感染很重要,已有几种疫苗获得批准。本研究旨在评估社区中新冠肺炎感染的发病密度,评估不同类型疫苗(灭活病毒、病毒载体或mRNA)的有效性以及不完全和完全接种的效率。在这项观察性横断面研究中,从2022年1月1日至2022年2月2日,共收到4924份样本,用于检测严重急性呼吸系统综合征冠状病毒2型。记录患者的年龄、性别和疫苗接种数据,并在通过PCR提取RNA后扩增s、N和ORF1ab基因。其中1034例(20.99%)为阳性。在1034例(20.99%)阳性病例中,418例和616例患者分别接种了疫苗和未接种疫苗。接种疫苗的患者中的严重急性呼吸系统综合征冠状病毒2型病例分为不完全和完全接种疫苗后的突然感染(≤10天)和晚期感染(≥10天)。接种疫苗可部分预防严重急性呼吸系统综合征冠状病毒2型感染。这可能是由于效力低,无法检测病毒蛋白质结构的最新变化。
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引用次数: 0
Efficiency Evaluation of Silver Nanoparticles in the Controlling of the Fungi Associated with the Date Palm Offshoots 纳米银对枣椰树分枝真菌的防治效果评价
Q4 Agricultural and Biological Sciences Pub Date : 2023-08-24 DOI: 10.53560/ppasb(60-3)788
Alaa O. Manea, Mohammed A. Fayyadh, Yehya A. Salih
Recently, Iraq has imported large numbers of tissue culture date palm offshoots from different countries. It is to build new orchards of date palm trees or plant them with the old orchards and some of them in the home's gardens. As a result of the widespread of many symptoms associated with these offshoots, this study was conducted in Basra Governorate, Iraq. To examine the capability of silver nanoparticles in controlling pathogens. The 36 fungi species were isolated from the shoot system of tissue culture date palm offshoots. Alternaria sp. was recorded at a high frequency compared to the Cladosporium spp. and Ulocladium spp. Neodieghtonia phoenicum, Scytalidium lignicola, and Neoscytalidium dimidiatum caused black scorch. Moreover, Phoma costarricensis has been recorded as causing the leaf spot disease. The roots infected by wilt disease have shown three various fungi, Fusarium solani, Fusarium proliferatum, and Fusarium fujikuroi. The study also illustrated that silver nanoparticles possessed a high ability to inhibit fungi growth in the laboratory.
最近,伊拉克从各国进口了大量组培椰枣枝。它是建立新的椰枣树果园或种植它们与旧果园,其中一些在家里的花园。由于与这些分支相关的许多症状普遍存在,本研究是在伊拉克巴士拉省进行的。研究银纳米颗粒控制病原体的能力。这36种真菌是从组织培养的枣椰树分枝的茎系中分离得到的。与枝孢霉属和乌氏霉属相比,交替孢霉属的记录频率较高。新狄氏霉属、木屑霉属和新狄氏霉属引起黑焦。此外,也有文献记载是引起叶斑病的病原菌。受枯萎病感染的根显示出三种不同的真菌,枯萎菌,增生镰刀菌和藤黑镰刀菌。该研究还表明,银纳米颗粒在实验室中具有很强的抑制真菌生长的能力。
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引用次数: 0
Assessment of Karachi as an Urban Heat Island Threat through Remote Sensing and GIS Techniques 基于遥感和GIS技术的卡拉奇城市热岛威胁评估
Q4 Agricultural and Biological Sciences Pub Date : 2023-08-24 DOI: 10.53560/ppasb(60-3)848
Muhammad Ali Lakhan1, Ambreen Afzal, Samreen Riaz Ahmed, Altaf Hussain Lahori, Muhammad Irfan, Salman Zubair, Anila Kausar, Shella Bano, Sergij Vambol, Viola Vambol, Igor Mishchenko
The present study aimed to assess the threat of transformation of Karachi into an Urban Heat Island, so, ambit was having calculated temperature, buildup areas, and normalized difference vegetation index through remote sensing and GIS techniques. The Landsat satellite data was used to differentiate the temperature in different years. These images were processed through Envi 4.7, Erdas Imagine, and ArcGIS 10.3.1. The results revealed that the maximum temperature was found up to 30.52, 35.25, 33.60, 46.73 °C; the buildup area was 23, 34, 26, 45 %; the NDVI results showed ranging from 0.224-1, 0.07-0.43, 0.201-1, 0.29-0.7 during this years. The average spatial land use temperature and buildup area increased by 1.03 and 1.9 times from 1990 to 2019. The maximum NDVI was observed during 2019, because of heavy rainfall as a result which supports promoting more greenery. With an increase in the buildup area, a significant change in the temperature of the territory was simultaneously observed. Therefore, this indicates a major task for urban developers extenuating the subsequent urban heat island occurrence. That is, for the first time it is scientifically substantiated and confirmed by the results that when creating a city development plan, it is extremely important to exclude the possibility of the urban heat island occurrence through preliminary studies. The practical value of the study lies in sound recommendations, one of which is the need for future urban development to emphasize urban plantings, including vertical forests to prevent UHI occurrence in the area of Karachi city.
本研究旨在评估卡拉奇转变为城市热岛的威胁,因此,范围是通过遥感和GIS技术计算温度、积累面积和归一化植被差指数。Landsat卫星数据用于区分不同年份的温度。这些图像通过Envi 4.7, Erdas Imagine, ArcGIS 10.3.1进行处理。结果表明:最高温度分别为30.52、35.25、33.60、46.73℃;堆积面积分别为23%、34%、26%、45%;年内NDVI值分别为0.224-1、0.07-0.43、0.201-1、0.29-0.7。1990 - 2019年,平均空间土地利用温度和建设面积分别增加了1.03倍和1.9倍。在2019年观测到最大的NDVI,因为强降雨支持促进更多的绿化。随着堆积面积的增加,同时观察到该区域的温度发生了显著变化。因此,这表明城市开发商的主要任务是减轻后续城市热岛的发生。也就是说,在制定城市发展规划时,通过初步研究排除城市热岛发生的可能性是极其重要的,这是第一次得到科学的证实和结果的证实。该研究的实用价值在于提出了合理的建议,其中之一是未来的城市发展需要强调城市种植,包括垂直森林,以防止卡拉奇市地区发生城市热岛。
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引用次数: 0
Zinc Oxide Nanoparticles mitigate toxic effects of Cadmium heavy metal in Chilli (Capsicum annuum L.) 氧化锌纳米颗粒减轻镉重金属对辣椒的毒性作用
Q4 Agricultural and Biological Sciences Pub Date : 2023-08-24 DOI: 10.53560/ppasb(60-3)854
Syed Mazhar Irfan, Khizar Hayat Bhatti
Heavy metals contaminated soils and water sources are one of the major global causes of inhibition of plant growth and productivity. Different strategies are being employed to overcome the challenging issue to increase plant yield requirements to fulfil the needs of future generations. The objective of the present study was to observe the effects of spray (foliar) of green synthesized ZnO nanoparticles (100 ppm), alone and its interaction in conjugation with Cd (Cd+ZnO-NPs) 100 ppm of both on the growth and biochemical activities of the target plant, i.e., two chilli varieties. After two weeks of transplant, treatments viz., Control (T1), ZnO nanoparticles 100 ppm (T2), Cd 100 ppm (T3), and ZnO nanoparticles 100 ppm + Cd 100 ppm (T4) were given for six weeks and different parameters of growth and biochemical analysis were made. Results have shown that 100 ppm foliar spray of ZnO-NPs has significantly increasing effects on root and shoot growth of chilli plants in alone (ZnO nanoparticles) and Combined (ZnO nanoparticles +Cd heavy metal) treatments mitigating toxic effects of Cd stress. A similar increase in values of total carbohydrates, soluble proteins, free amino acids, and photosynthetic pigments were observed mostly in a combination of Cd+ZnO-NPs treatment showing remediation properties of ZnO nanoparticles against Cd stress in chilli plant. In conclusion, it may be suggested that 100 ppm ZnO-NPs foliar spray can have an increasing effect on the growth parameters of the plants under stressful conditions of Cd heavy metal.
重金属污染的土壤和水源是抑制植物生长和生产力的主要全球原因之一。正在采用不同的策略来克服这一具有挑战性的问题,以提高植物产量需求,满足子孙后代的需求。本研究的目的是观察单独喷洒(叶面)绿色合成的ZnO纳米颗粒(100ppm)及其与100ppm Cd(Cd+ZnO纳米颗粒)结合对目标植物(即两个辣椒品种)生长和生化活性的影响。移植两周后,给予对照组(T1)、ZnO纳米颗粒100ppm(T2)、Cd 100ppm(T3)和ZnO纳米颗粒100 ppm+Cd 100ppm(T4)处理六周,并进行不同的生长参数和生化分析。结果表明,在单独(ZnO纳米粒子)和联合(ZnO纳米粒子+Cd重金属)处理中,100ppm的ZnO纳米粒子叶面喷雾对辣椒植株的根和地上部生长具有显著的增加作用,减轻了Cd胁迫的毒性影响。总碳水化合物、可溶性蛋白质、游离氨基酸和光合色素的值也有类似的增加,主要是在Cd+ZnO纳米颗粒处理的组合中观察到的,这表明ZnO纳米颗粒对辣椒植株镉胁迫的修复性能。总之,在镉重金属胁迫条件下,100ppm ZnO NPs叶面喷雾可以提高植物的生长参数。
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引用次数: 0
Space-borne Air Quality Monitoring of Nitrogen dioxide (NO2) over Karachi and Lahore using Remote Sensing Tools 利用遥感工具监测卡拉奇和拉合尔上空二氧化氮(NO2)的空载空气质量
Q4 Agricultural and Biological Sciences Pub Date : 2023-08-24 DOI: 10.53560/ppasb(60-3)875
Rabbia Rehmat, Lubna Rafique, Muhammad Irfan, Samreen Riaz Ahmed, Altaf Hussain Lahori, Aqsa Muhammadi, Muhammad Taha, Sergij Vambol, Mykhailo Shulga
In this study, we used Sentinel-5P TROPOMI satellite data to examine the NO2 and gas concentrations in the cities of Lahore and Karachi, Pakistan, and to use environmental valuation methods that focus on air quality problems. Furthermore, the causes and main sources of NO2 are discussed with its effect on the environment and the health of humans. This study examines the correlation between the tropospheric NO2 collected from the recently launched Sentinel-5 Precursor, a low-earth-orbit atmospheric mission dedicated to observing air pollution and outfitted with the spectrometer TROPOMI (Tropospheric Monitoring Instrument). The average amount of NO2 that was gathered between May 2018 and May 2022. The results showed higher levels of NO2 concentrations were recorded in both, Karachi and Lahore. The concentrations exceed the WHO standard levels for NO2 in ambient air. The NO2 concentrations in Karachi ranged from 3.0e-6 mol/m2 being the minimum average concentration to 4.0e-1 mol/m2 being the maximum concentration. However, in Lahore, the minimum average value of NO2 was ranging from 4.0e-5 mol/m2 to 5.5e-1 mol/m2 as the maximum average, which was higher than the minimum and maximum values of Karachi. The study also revealed that the NO2 concentrations measured for both cities were higher than the WHO's yearly limit threshold, which is 53 ppb/year. Thus, it was crucial to take action to address this issue before it poses a severe risk to the local people. This study's identification of the key regions with the greatest NO2 concentrations will aid in understanding the significance of satellite data for monitoring NO2 concentration. Thus, the originality of the study lies in the fact that using the example of Karachi and Lahore, the dynamics of the deterioration of the environmental situation was revealed, and the main reasons for what was happening were also established. In this case, an available tool was used - remote sensing tools. The competent authorities can assist this study in managing and regulating the air quality in the most densely populated areas.
在这项研究中,我们使用Sentinel-5P TROPOMI卫星数据来检查巴基斯坦拉合尔和卡拉奇市的NO2和气体浓度,并使用关注空气质量问题的环境评估方法。此外,还讨论了NO2的产生原因、主要来源及其对环境和人类健康的影响。这项研究考察了从最近发射的哨兵5号前体收集的对流层NO2之间的相关性,哨兵5号是一项近地轨道大气任务,专门用于观测空气污染,并配备了光谱仪TROPOMI(对流层监测仪器)。2018年5月至2022年5月期间收集的NO2平均量。结果显示,卡拉奇和拉合尔的NO2浓度都较高。环境空气中的NO2浓度超过世界卫生组织标准水平。卡拉奇的NO2浓度范围从3.0e-6mol/m2(最小平均浓度)到4.0e-1mol/m2(最大浓度)。然而,在拉合尔,NO2的最小平均值为4.0e-5mol/m2,最大平均值为5.5e-1mol/m2,高于卡拉奇的最小值和最大值。研究还显示,这两个城市的NO2浓度都高于世界卫生组织的年度限值阈值,即53 ppb/年。因此,在这一问题对当地人民构成严重风险之前,采取行动解决这一问题至关重要。这项研究确定了NO2浓度最高的关键区域,这将有助于理解卫星数据对监测NO2浓度的重要性。因此,该研究的独创性在于,以卡拉奇和拉合尔为例,揭示了环境状况恶化的动态,并确定了发生这种情况的主要原因。在这种情况下,使用了一种可用的工具——遥感工具。主管当局可以协助这项研究管理和调节人口最稠密地区的空气质量。
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引用次数: 2
Vertical Farming of Horticulture Crops: A Recent Trend 园艺作物垂直种植:近期趋势
Q4 Agricultural and Biological Sciences Pub Date : 2023-08-24 DOI: 10.53560/ppasb(60-3)701
Pallvi Verma, Manish Bakshi, Lakshya
Vertical farming now a day is gaining status as complementary to traditional farming practices, allowing for more sustainable food production for the world's growing population. While early studies on vertical farming systems focused primarily on the advancement of technology through innovative designs, hydroponic cultivation automation monitoring, and the use of advanced LED lighting systems, more recent studies have focused on the resilience and circularity of vertical farming. Over the last few decades, there have been elevated concerns about ethical cultivation practices and environmental issues, reliance on non renewable resources, commitment to biodiversity conservation, ration scarcity, and its leverage to hunger. Environmental issues, such as economic justice issues, biodiversity commitments, and a focus on food security issues, have gained traction in social work. Vertical farming is one of the solutions to many problems including food and nutritional security, environment safety, resource utilization, land fragmentation, climate resilience agriculture, etc.
垂直农业如今日益成为传统农业实践的补充,为世界上不断增长的人口提供了更可持续的粮食生产。虽然早期对垂直农业系统的研究主要集中在通过创新设计、水培种植自动化监控和使用先进的LED照明系统来提高技术,但最近的研究集中在垂直农业的弹性和循环性上。在过去的几十年里,人们越来越担心道德种植做法和环境问题、对不可再生资源的依赖、对生物多样性保护的承诺、口粮短缺及其对饥饿的影响。环境问题,如经济正义问题、生物多样性承诺和对粮食安全问题的关注,在社会工作中得到了重视。垂直农业是解决许多问题的方法之一,包括粮食和营养安全、环境安全、资源利用、土地分割、气候适应性农业等。
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引用次数: 0
Influence of Basal Salts, Sucrose and Plant Growth Regulator Levels on Nucellar Embryogenesis and Plantlet Regeneration in Monoembryonic Mango Varieties 基盐、蔗糖和植物生长调节剂水平对芒果单胚品种裸胚发生和植株再生的影响
Q4 Agricultural and Biological Sciences Pub Date : 2023-08-24 DOI: 10.53560/ppasb(60-3)842
N. Solangi, Mushtaque Ahmed Jatoi, Abdul Aziz Mirani, Waheed Ali Mirbahar, M. A. Solangi, A. Abul-soad, G. S. Markhand
Current study described stage-wise protocols for in vitro propagation of commercially important varieties of mango. Induction of somatic embryos (SE) and plantlet regeneration was obtained using nucellar explants of three superior monoembryonic mango vars.‘Saroli’, ‘Langra’, and ‘Chaunsa’ were cultivated in Khairpur, Pakistan. The immature fruits (2.5-4.0 cm long) were surface disinfected using a 30% sodium hypochlorite (NaOCl) solution. Results revealed that significantly highest direct somatic embryogenesis (93%) was obtained in var. ‘Chaunsa’ under full dark on culture medium comprising of 2.0 mg L-1 N6 2-isopentenyl adenine (2iP), 0.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D). Medium consisted of 2iP 4.0 mg L-1, 2,4-D 1.0 mg L-1 induced significantly highest embryogenic callus (91%) using nucellar explants in var.‘Chaunsa’. Significantly highest germination (95%) of SE was achieved in var. ‘Chaunsa’ on the medium comprising microsalts of MS, macrosalts of B5, 2iP 0.1 mg L-1, Kinetin (Kin) 0.5 mg L-1. Highest shoot length (5.1 cm) and root length (4 cm) were obtained in var. ‘Langra’ on the medium consisted of microsalts of MS, macrosalts of B5, 30 g L-1 sucrose, 200 mg L-1 activated charcoal (AC), 0.1 mg L-1 naphthalene acetic acid (NAA), 0.2 mg L-1 benzyl adenine (BA). Stage-wise protocols established for the regeneration of plantlets can be useful for micropropagation of the other mango varieties of the world.
目前的研究描述了商业上重要的芒果品种的体外繁殖的分阶段方案。利用3个优质单胚芒果品种的心心外植体进行了体细胞胚诱导和植株再生。“Saroli”,“langa”和“Chaunsa”在巴基斯坦的Khairpur种植。未成熟果实(2.5-4.0 cm长)用30%次氯酸钠(NaOCl)溶液表面消毒。结果表明,在含有2.0 mg L-1 N6 - 2-异戊烯腺嘌呤(2iP)和0.5 mg L-1 2,4-二氯苯氧乙酸(2,4- d)的培养基中,全暗条件下,‘Chaunsa’品种的直接体细胞胚发生率最高(93%)。2iP 4.0 mg l - 1,2,4 - d1.0 mg L-1的培养基诱导的‘Chaunsa’品种珠心外植体胚性愈伤组织最高(91%)。在含有MS微盐、B5大盐、2iP 0.1 mg L-1、Kinetin (Kin) 0.5 mg L-1的培养基上,‘Chaunsa’品种的SE萌发率最高(95%)。在MS微盐、B5大盐、30 g L-1蔗糖、200 mg L-1活性炭(AC)、0.1 mg L-1萘乙酸(NAA)、0.2 mg L-1苄基腺嘌呤(BA)培养基上,‘Langra’的茎长最高,为5.1 cm,根长最高,为4 cm。为植株再生建立的分阶段方案可用于世界上其他芒果品种的微繁。
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Proceedings of the Pakistan Academy of Sciences: Part B
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