Pub Date : 2019-01-01DOI: 10.1515/bglass-2019-0006
Steven B. Jung, T. Day, T. Boone, Brenton Buziak, A. Omar
Abstract Bioactive glass was first developed in the late 1960’s as a compound that would facilitate bone re-growth. In more recent years, this technology has been used to promote wound healing through its ability to stimulate soft tissue growth, angiogenesis, reduce inflammation, and prevent infection. Chronically infected wounds, which result from biofilm formation, affect millions of patients in the Unites States each year and cost billions of dollars to treat. The present studies demonstrate exposure of pre-formed biofilms to bioactive glass, under simulated body conditions, resulted in significant reduction in bacterial load. Additionally, specific therapeutically active metal ions such as copper and zinc were added to a borate bioactive glass formulation through a process of ion doping and found to further enhance the anti-biofilm activity. Based on the present findings, the antibiofilm agents released by borate bioactive glasses may prove effective to eradicate the biofilm infections that prevent healing in patients with chronic wounds.
{"title":"Anti-biofilm activity of two novel, borate based, bioactive glass wound dressings","authors":"Steven B. Jung, T. Day, T. Boone, Brenton Buziak, A. Omar","doi":"10.1515/bglass-2019-0006","DOIUrl":"https://doi.org/10.1515/bglass-2019-0006","url":null,"abstract":"Abstract Bioactive glass was first developed in the late 1960’s as a compound that would facilitate bone re-growth. In more recent years, this technology has been used to promote wound healing through its ability to stimulate soft tissue growth, angiogenesis, reduce inflammation, and prevent infection. Chronically infected wounds, which result from biofilm formation, affect millions of patients in the Unites States each year and cost billions of dollars to treat. The present studies demonstrate exposure of pre-formed biofilms to bioactive glass, under simulated body conditions, resulted in significant reduction in bacterial load. Additionally, specific therapeutically active metal ions such as copper and zinc were added to a borate bioactive glass formulation through a process of ion doping and found to further enhance the anti-biofilm activity. Based on the present findings, the antibiofilm agents released by borate bioactive glasses may prove effective to eradicate the biofilm infections that prevent healing in patients with chronic wounds.","PeriodicalId":37354,"journal":{"name":"Biomedical Glasses","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bglass-2019-0006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49186845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.1515/bglass-2019-0016
R. Borges, Natália Del Rey Menezes, J. Marchi
Abstract Rare Earth (RE)-containing bioactive glasses has recently gained more relevance in the field of biomaterials due to the unique optical, electronic, and chemical properties of the RE elements. In this work, the influence of gadolinium in the thermal properties of bioactive glasses based on the SiO2-Na2O-CaO-P2O5 containing different Na/Ca ratio was studied. The glasses were obtained by melting-quenching, and their properties were evaluated by differential thermal analysis, X-ray diffraction, and scanning electron microscopy. The results evidenced that Gd tends to increase the working temperature (Tc-Tg) in Ca-rich glasses, which leads to a less tendency to devitrification. On the other hand, in Na-rich glasses, Gd did not play an essential role in crystallization, and the thermal properties of Gd-containing Na-rich glasses were similar to their counterparts. In conclusion, we suggested that it is possible to control the influence of Gd in the glass devitrification by changing the Na/Ca ratio in the glass composition. These results may be interesting for further works to which crystallization may be either a desired or undesired phenomena.
摘要含稀土(RE)生物活性玻璃由于其独特的光学、电子和化学性质，近年来在生物材料领域获得了越来越多的关注。本文研究了钆对不同Na/Ca比的sio2 - na20 - cao - p2o5生物活性玻璃热性能的影响。通过熔融淬火法制备了玻璃，并通过差热分析、x射线衍射和扫描电镜对其性能进行了评价。结果表明，Gd会使富钙玻璃的工作温度(Tc-Tg)升高，从而使玻璃的反玻璃化倾向减小。另一方面，在富na玻璃中，Gd在结晶过程中没有发挥重要作用，含Gd富na玻璃的热性能与同类玻璃相似。综上所述，我们认为可以通过改变玻璃组成中的Na/Ca比来控制Gd对玻璃脱氮的影响。这些结果对于结晶可能是一种期望或不希望的现象的进一步工作可能是有趣的。
{"title":"The influence of gadolinium on the thermal properties of bioactive glasses","authors":"R. Borges, Natália Del Rey Menezes, J. Marchi","doi":"10.1515/bglass-2019-0016","DOIUrl":"https://doi.org/10.1515/bglass-2019-0016","url":null,"abstract":"Abstract Rare Earth (RE)-containing bioactive glasses has recently gained more relevance in the field of biomaterials due to the unique optical, electronic, and chemical properties of the RE elements. In this work, the influence of gadolinium in the thermal properties of bioactive glasses based on the SiO2-Na2O-CaO-P2O5 containing different Na/Ca ratio was studied. The glasses were obtained by melting-quenching, and their properties were evaluated by differential thermal analysis, X-ray diffraction, and scanning electron microscopy. The results evidenced that Gd tends to increase the working temperature (Tc-Tg) in Ca-rich glasses, which leads to a less tendency to devitrification. On the other hand, in Na-rich glasses, Gd did not play an essential role in crystallization, and the thermal properties of Gd-containing Na-rich glasses were similar to their counterparts. In conclusion, we suggested that it is possible to control the influence of Gd in the glass devitrification by changing the Na/Ca ratio in the glass composition. These results may be interesting for further works to which crystallization may be either a desired or undesired phenomena.","PeriodicalId":37354,"journal":{"name":"Biomedical Glasses","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42374863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.1515/bglass-2019-0007
Adja B. R. Touré, E. Mele, J. K. Christie
Abstract Here, molecular dynamics simulations have been carried out on phosphate glasses to clarify the previously debated influence of fluoride on the bioactivity of these glasses. We developed a computationally advanced inter-atomic force field including polarisation effects of the fluorine and oxygen atoms. Structural characterisations of the simulated systems showed that fluoride ions exclusively bond to the calcium modifier cations creating clusters within the glass structure and therefore decreasing the bioactivity of fluoridated phosphate glasses, making them less suitable for biomedical applications.
{"title":"Atomic-scale clustering inhibits the bioactivity of fluoridated phosphate glasses","authors":"Adja B. R. Touré, E. Mele, J. K. Christie","doi":"10.1515/bglass-2019-0007","DOIUrl":"https://doi.org/10.1515/bglass-2019-0007","url":null,"abstract":"Abstract Here, molecular dynamics simulations have been carried out on phosphate glasses to clarify the previously debated influence of fluoride on the bioactivity of these glasses. We developed a computationally advanced inter-atomic force field including polarisation effects of the fluorine and oxygen atoms. Structural characterisations of the simulated systems showed that fluoride ions exclusively bond to the calcium modifier cations creating clusters within the glass structure and therefore decreasing the bioactivity of fluoridated phosphate glasses, making them less suitable for biomedical applications.","PeriodicalId":37354,"journal":{"name":"Biomedical Glasses","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bglass-2019-0007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46473825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.1515/bglass-2019-0005
Yunqi Wang, Chenkai Zhu, A. Parsons, C. Rudd, I. Ahmed, N. Sharmin
Abstract Four phosphate-based glass formulations in the system P45Mg24Ca16Na(15−x)Znx, referred to as P45Znx (x = 0, 5, 10 and 15 mol%), were prepared using a melt quenching process. The effect of ZnO addition on density, molar volume, thermal properties and degradation rates were studied. An increase in the glass transition, crystallisation, melting and liquidus temperatures were seen when replacing Na2O with ZnO. The molar volume of the bulk glasses was seen to decrease with increasing ZnO content. The dissolution rate of the zinc-free glass was 2.48 × 10−8 kg m−2 s−1 and addition of 5 mol% ZnO resulted in a reduction of the dissolution rate to 1.68 × 10−8 kg m−2 s−1. However, further addition of ZnO from 5 mol% to 15 mol% increased the dissolution rate of the glass system. The glasses were deliberately crystallised and XRD studies identified the Z n2P2O7 phase for glass code P45Zn5, and Zn(PO3)2 phase for P45Zn10 and P45Zn15 glasses. Cyto-compatibility studies were conducted using MG63 cells for 14 days. An overall increase in the metabolic activity and DNA concentration of cells was seen from day 1 to day 14 for all glass formulations investigated. However, increasing ZnO content from 0 to 15 mol% seemed to have a negative effect on the cellular activity. Interestingly, a remarkably higher ALP activity was seen at day 14 for glass codes P45Zn5 and P45Zn10 in comparison with the TCP control and the P45Zn0 glass.
摘要采用熔融淬火工艺制备了P45Mg24Ca16Na（15−x）Znx体系中的四种磷酸盐基玻璃配方，称为P45Znx（x=0、5、10和15mol%）。研究了添加ZnO对密度、摩尔体积、热性能和降解速率的影响。当用ZnO代替Na2O时，观察到玻璃化转变、结晶、熔融和液相线温度的增加。大块玻璃的摩尔体积随着ZnO含量的增加而减小。无锌玻璃的溶解速率为2.48×10−8 kg m−2 s−1，添加5 mol%ZnO使溶解速率降低至1.68×。然而，从5mol%到15mol%的ZnO的进一步添加增加了玻璃系统的溶解速率。玻璃被有意结晶，XRD研究确定了玻璃代码P45Zn5的Z n2P2O7相，以及P45Zn10和P45Zn15玻璃的Zn（PO3）2相。使用MG63细胞进行为期14天的细胞相容性研究。对于所研究的所有玻璃制剂，从第1天到第14天观察到细胞的代谢活性和DNA浓度的总体增加。然而，将ZnO含量从0摩尔%增加到15摩尔%似乎对细胞活性有负面影响。有趣的是，与TCP对照和P45Zn0玻璃相比，在第14天观察到玻璃代码P45Zn5和P45Zn 10的ALP活性显著更高。
{"title":"Effects of ZnO addition on thermal properties, degradation and biocompatibility of P45Mg24Ca16Na(15−x)Znx glasses","authors":"Yunqi Wang, Chenkai Zhu, A. Parsons, C. Rudd, I. Ahmed, N. Sharmin","doi":"10.1515/bglass-2019-0005","DOIUrl":"https://doi.org/10.1515/bglass-2019-0005","url":null,"abstract":"Abstract Four phosphate-based glass formulations in the system P45Mg24Ca16Na(15−x)Znx, referred to as P45Znx (x = 0, 5, 10 and 15 mol%), were prepared using a melt quenching process. The effect of ZnO addition on density, molar volume, thermal properties and degradation rates were studied. An increase in the glass transition, crystallisation, melting and liquidus temperatures were seen when replacing Na2O with ZnO. The molar volume of the bulk glasses was seen to decrease with increasing ZnO content. The dissolution rate of the zinc-free glass was 2.48 × 10−8 kg m−2 s−1 and addition of 5 mol% ZnO resulted in a reduction of the dissolution rate to 1.68 × 10−8 kg m−2 s−1. However, further addition of ZnO from 5 mol% to 15 mol% increased the dissolution rate of the glass system. The glasses were deliberately crystallised and XRD studies identified the Z n2P2O7 phase for glass code P45Zn5, and Zn(PO3)2 phase for P45Zn10 and P45Zn15 glasses. Cyto-compatibility studies were conducted using MG63 cells for 14 days. An overall increase in the metabolic activity and DNA concentration of cells was seen from day 1 to day 14 for all glass formulations investigated. However, increasing ZnO content from 0 to 15 mol% seemed to have a negative effect on the cellular activity. Interestingly, a remarkably higher ALP activity was seen at day 14 for glass codes P45Zn5 and P45Zn10 in comparison with the TCP control and the P45Zn0 glass.","PeriodicalId":37354,"journal":{"name":"Biomedical Glasses","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bglass-2019-0005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42376361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-01DOI: 10.1515/bglass-2018-0012
Shiva Naseri, William C. Lepry, M. S. Mohammadi, K. Waters, S. Nazhat
Abstract The chemical durability of phosphate-based glasses (PGs) in an aqueous environment is crucial in determining their dissolution properties and their ultimate performance in vivo. In this study, inverse gas chromatography (IGC) and dynamic vapour sorption (DVS)were used to investigate the short-term aqueous interactions of PG particles doped with SiO2 and TiO2 (50P2O5-40CaO-xSiO2- (10-x)TiO2, where x=7, 5, 3, and 0 mol%). IGC was used to evaluate the solubility parameter and surface energy of PGs. A good correlation between the polar parts of the solubility parameter and surface energy with glass transition temperature (Tg) and dissolution rates was demonstrated. DVS was applied to monitor the sorption characteristics of the PG particles. An increase in silica content resulted in greater vapour sorption and mass change. Nuclear magnetic resonance spectroscopy data of the PGs post exposure to vapour demonstrated that increased SiO2 content disrupted the glass network and formed protonated phosphate species. Fourier transform infrared spectroscopy verified the presence of non-reacted water molecules in the PGs depending on SiO2 content. Moreover, there was a good correlation between the values measured through IGC and DVS, demonstrating the ability of both techniques in predicting the dissolution properties of PGs as consequence of alterations in their chemistry.
{"title":"Surface Properties and Reactivity of Phosphate-based Glasses by Inverse Gas Chromatography and Dynamic Vapour Sorption","authors":"Shiva Naseri, William C. Lepry, M. S. Mohammadi, K. Waters, S. Nazhat","doi":"10.1515/bglass-2018-0012","DOIUrl":"https://doi.org/10.1515/bglass-2018-0012","url":null,"abstract":"Abstract The chemical durability of phosphate-based glasses (PGs) in an aqueous environment is crucial in determining their dissolution properties and their ultimate performance in vivo. In this study, inverse gas chromatography (IGC) and dynamic vapour sorption (DVS)were used to investigate the short-term aqueous interactions of PG particles doped with SiO2 and TiO2 (50P2O5-40CaO-xSiO2- (10-x)TiO2, where x=7, 5, 3, and 0 mol%). IGC was used to evaluate the solubility parameter and surface energy of PGs. A good correlation between the polar parts of the solubility parameter and surface energy with glass transition temperature (Tg) and dissolution rates was demonstrated. DVS was applied to monitor the sorption characteristics of the PG particles. An increase in silica content resulted in greater vapour sorption and mass change. Nuclear magnetic resonance spectroscopy data of the PGs post exposure to vapour demonstrated that increased SiO2 content disrupted the glass network and formed protonated phosphate species. Fourier transform infrared spectroscopy verified the presence of non-reacted water molecules in the PGs depending on SiO2 content. Moreover, there was a good correlation between the values measured through IGC and DVS, demonstrating the ability of both techniques in predicting the dissolution properties of PGs as consequence of alterations in their chemistry.","PeriodicalId":37354,"journal":{"name":"Biomedical Glasses","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bglass-2018-0012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48246457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-01DOI: 10.1515/bglass-2018-0011
S. Labbaf, A. B. Houreh, M. Rahimi, Hungkit Ting, J.R. Jones, M. Nasr-Esfahani
Abstract The study reports the fabrication and in vitro biological evaluation of a sol-gel derived bioactive glass (BG) / polycaprolactone (PCL) composite fiber membrane, as a potential candidate for bone regeneration applications. The non woven composite mats were prepared by introducing the glass particles into the electrospinning process. Adding the glass improved the homogeneity of the fibers. The apatite forming ability of the membranes in simulated body fluid were evaluated and showed that hydroxyapatite had formed within 21 days in SBF and completely covered the surface of the membrane. In cell culture, dental pulp stem cells adhered proliferated and produced mineralized matrix on the PCL/BG fiber membrane.
{"title":"Bioactive glass-polycaprolactone fiber membrane and response of dental pulp stem cells in vitro","authors":"S. Labbaf, A. B. Houreh, M. Rahimi, Hungkit Ting, J.R. Jones, M. Nasr-Esfahani","doi":"10.1515/bglass-2018-0011","DOIUrl":"https://doi.org/10.1515/bglass-2018-0011","url":null,"abstract":"Abstract The study reports the fabrication and in vitro biological evaluation of a sol-gel derived bioactive glass (BG) / polycaprolactone (PCL) composite fiber membrane, as a potential candidate for bone regeneration applications. The non woven composite mats were prepared by introducing the glass particles into the electrospinning process. Adding the glass improved the homogeneity of the fibers. The apatite forming ability of the membranes in simulated body fluid were evaluated and showed that hydroxyapatite had formed within 21 days in SBF and completely covered the surface of the membrane. In cell culture, dental pulp stem cells adhered proliferated and produced mineralized matrix on the PCL/BG fiber membrane.","PeriodicalId":37354,"journal":{"name":"Biomedical Glasses","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bglass-2018-0011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48319564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.1515/bglass-2018-0010
Cédric Bossard, Henri Granel, Y. Wittrant, É. Jallot, J. Lao, C. Vial, H. Tiainen
Abstract Bioactive glasses (BG) bond to bone and stimulate bone regeneration, but they are brittle. Inorganicorganic hybrids appear as promising bone substitutes since they associate the bone mineral forming ability of BG with the toughness of polymers. Hybrids comprised of polycaprolactone (PCL) and SiO2-CaO BG were produced by sol-gel chemistry and processed into porous scaffolds with controlled pore and interconnection sizes. The obtained scaffolds are highly flexible, meaning that PCL effectively introduces toughness. Apatite formation is observed within 24 hours of immersion in simulated body fluid (SBF) and is not limited to the surface as the entire hybrid progressively changes into bone-like minerals. The degradation rate is suitable for bone regeneration with a 13.2% weight loss after 8 weeks of immersion. Primary osteoblasts cultured in scaffolds demonstrate that the samples are not cytotoxic and provide good cell adhesion. The in vivo study confirms the bioactivity, biocompatibility and suitable degradation rate of the hybrid. A physiological bone made of trabeculae and bone marrow regenerates. The structure and kinetic of bone regeneration was similar to the implanted commercial standard based on bovine bone, demonstrating that this new synthetic PCL-BG hybrid could perform as well as animal-derived bone substitutes.
{"title":"Polycaprolactone / bioactive glass hybrid scaffolds for bone regeneration","authors":"Cédric Bossard, Henri Granel, Y. Wittrant, É. Jallot, J. Lao, C. Vial, H. Tiainen","doi":"10.1515/bglass-2018-0010","DOIUrl":"https://doi.org/10.1515/bglass-2018-0010","url":null,"abstract":"Abstract Bioactive glasses (BG) bond to bone and stimulate bone regeneration, but they are brittle. Inorganicorganic hybrids appear as promising bone substitutes since they associate the bone mineral forming ability of BG with the toughness of polymers. Hybrids comprised of polycaprolactone (PCL) and SiO2-CaO BG were produced by sol-gel chemistry and processed into porous scaffolds with controlled pore and interconnection sizes. The obtained scaffolds are highly flexible, meaning that PCL effectively introduces toughness. Apatite formation is observed within 24 hours of immersion in simulated body fluid (SBF) and is not limited to the surface as the entire hybrid progressively changes into bone-like minerals. The degradation rate is suitable for bone regeneration with a 13.2% weight loss after 8 weeks of immersion. Primary osteoblasts cultured in scaffolds demonstrate that the samples are not cytotoxic and provide good cell adhesion. The in vivo study confirms the bioactivity, biocompatibility and suitable degradation rate of the hybrid. A physiological bone made of trabeculae and bone marrow regenerates. The structure and kinetic of bone regeneration was similar to the implanted commercial standard based on bovine bone, demonstrating that this new synthetic PCL-BG hybrid could perform as well as animal-derived bone substitutes.","PeriodicalId":37354,"journal":{"name":"Biomedical Glasses","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bglass-2018-0010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43249741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.1515/bglass-2018-0009
N. Esfahanizadeh, M. Nourani, A. Bahador, N. Akhondi, M. Montazeri
Abstract Colonization of periodontal pathogens on the surgical sites is one of the primary reasons for the failure of regenerative periodontal therapies. Bioactive glasses (BGs) owing to their favorable structural and antimicrobial properties have been proposed as promising materials for the reconstruction of periodontal and peri-implant bone defects. This study aimed to investigate the antibiofilm activity of zinc-doped BG (Zn/BG) compared with 45S5 Bioglass® (BG®) on putative periodontal pathogens. In this in vitro experimental study, the nano BG doped with 5-mol% zinc and BG® were synthesized by sol-gel method. Mono-species biofilms of Aggregatibacter actinomycetemcomitans (A. a), Porphyromonas gingivalis (P. g), and Prevotella intermedia (P. i)were prepared separately in a well-containing microplate. After 48 hours of exposure to generated materials at 37°C, the anti-biofilm potential of the samples was studied by measuring the optical density (OD) at 570nm wavelengths with a microplate reader. Two-way ANOVA then analyzed the results. Both Zn/BG and BG® significantly reduced the biofilm formation ability of all examined strains after 48 hours of incubation (P=0.0001). Moreover, the anti-biofilm activity of Zn/BG was significantly stronger than BG® (P=0.0001), which resulted in the formation of a weak biofilm (OD<1) compared with a moderately adhered biofilm observed with BG® (1
{"title":"The Anti-biofilm Activity of Nanometric Zinc doped Bioactive Glass against Putative Periodontal Pathogens: An in vitro Study","authors":"N. Esfahanizadeh, M. Nourani, A. Bahador, N. Akhondi, M. Montazeri","doi":"10.1515/bglass-2018-0009","DOIUrl":"https://doi.org/10.1515/bglass-2018-0009","url":null,"abstract":"Abstract Colonization of periodontal pathogens on the surgical sites is one of the primary reasons for the failure of regenerative periodontal therapies. Bioactive glasses (BGs) owing to their favorable structural and antimicrobial properties have been proposed as promising materials for the reconstruction of periodontal and peri-implant bone defects. This study aimed to investigate the antibiofilm activity of zinc-doped BG (Zn/BG) compared with 45S5 Bioglass® (BG®) on putative periodontal pathogens. In this in vitro experimental study, the nano BG doped with 5-mol% zinc and BG® were synthesized by sol-gel method. Mono-species biofilms of Aggregatibacter actinomycetemcomitans (A. a), Porphyromonas gingivalis (P. g), and Prevotella intermedia (P. i)were prepared separately in a well-containing microplate. After 48 hours of exposure to generated materials at 37°C, the anti-biofilm potential of the samples was studied by measuring the optical density (OD) at 570nm wavelengths with a microplate reader. Two-way ANOVA then analyzed the results. Both Zn/BG and BG® significantly reduced the biofilm formation ability of all examined strains after 48 hours of incubation (P=0.0001). Moreover, the anti-biofilm activity of Zn/BG was significantly stronger than BG® (P=0.0001), which resulted in the formation of a weak biofilm (OD<1) compared with a moderately adhered biofilm observed with BG® (1<OD<2). Zn/BG showed a significant inhibitory effect on the biofilm formation of all examined periodontal pathogens. Given the enhanced regenerative and anti-biofilm properties of this novel biomaterial, further investigations are required for its implementation in clinical situations.","PeriodicalId":37354,"journal":{"name":"Biomedical Glasses","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bglass-2018-0009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46101839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-01DOI: 10.1515/bglass-2018-0007
J. Hum, Shiva Naseri, A. Boccaccini
Abstract The present study has focused on the development of new composite scaffolds based on the combination of zein with bioactive glass for the application in bone tissue engineering. Porous polymeric matrices were produced by the salt leaching technique. By incorporating 45S5 bioactive glass particles the lack of bioactivity can be remedied. However, the addition of bioactive glass is influencing the plasticization behavior of the zein matrix during the salt leaching which negatively affects the compression strength as well as the degradation behavior. This paper describes the process during leaching and explains the different behavior of zein with and without the presence of bioactive glass
{"title":"Bioactive glass combined with zein as composite material for the application in bone tissue engineering","authors":"J. Hum, Shiva Naseri, A. Boccaccini","doi":"10.1515/bglass-2018-0007","DOIUrl":"https://doi.org/10.1515/bglass-2018-0007","url":null,"abstract":"Abstract The present study has focused on the development of new composite scaffolds based on the combination of zein with bioactive glass for the application in bone tissue engineering. Porous polymeric matrices were produced by the salt leaching technique. By incorporating 45S5 bioactive glass particles the lack of bioactivity can be remedied. However, the addition of bioactive glass is influencing the plasticization behavior of the zein matrix during the salt leaching which negatively affects the compression strength as well as the degradation behavior. This paper describes the process during leaching and explains the different behavior of zein with and without the presence of bioactive glass","PeriodicalId":37354,"journal":{"name":"Biomedical Glasses","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bglass-2018-0007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49120829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-01DOI: 10.1515/bglass-2018-0006
Chao Tan, I. Ahmed, A. Parsons, Chenkai Zhu, F. Betanzos, C. Rudd, Xiaoling Liu
Abstract This paper investigated the preparation of phosphate glass fibres (PGFs) in the following systems: i) 45P2O5-5B2O3-5Na2O-(29-x)CaO-16MgO-(x)Fe2O3 and ii) 45P2O5-5B2O3-5Na2O-24CaO-(21-x)MgO-(x)Fe2O3 (where x = 5, 8 and 11 mol%) for biomedical applications. Continuous fibres of 23 ± 1 μm diameter were prepared via a meltdraw spinning process. Compositions with higher Fe2O3 content and higher MgO/CaO ratio required higher melting temperature and longer heating time to achieve glass melts for fibre pulling. The effects of Fe2O3 addition and annealing treatment on mechanical properties and degradation behaviours were also investigated. Adding Fe2O3 was found to increase the tensile strength from 523 ± 63 (Ca-Fe5) to 680 ± 75 MPa (Ca-Fe11), improve the tensile modulus from72 ± 4 (Ca-Fe5) to 78 ± 3 GPa (Ca-Fe11) and decrease the degradation rate from 4.0 (Mg-Fe5) to 1.9 × 10−6 kg m−2 s−1 (Mg-Fe11). The annealing process reduced the fibre tensile strength by 46% (Ca-Fe5), increased the modulus by 19.6%(Ca-Fe8) and decreased the degradation rate by 89.5% (Mg-Fe11) in comparison to the corresponding as drawn fibres. Additionally, the annealing process also impeded the formation of precipitate shells and revealed coexistence of the precipitation and the pitting corrosion as fibre degradation behaviours.
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