Lívia da Silva Freitas, C. Caprara, L. Volcão, Rodrigo de Lima Brum, Israel Barbosa, F. M. R. da Silva, D. Ramos
{"title":"Usnic Acid (+) Enantiomer in Alternative In Vitro Control of Burkholderia cepacia and Allelopathic Effect","authors":"Lívia da Silva Freitas, C. Caprara, L. Volcão, Rodrigo de Lima Brum, Israel Barbosa, F. M. R. da Silva, D. Ramos","doi":"10.1089/aivt.2021.0001","DOIUrl":"https://doi.org/10.1089/aivt.2021.0001","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45210522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.1089/aivt.2022.29028.editorial
Andrew Gow
{"title":"Seeing Through the Smoke: The Importance of Toxicological Testing in Nicotine Delivery Systems","authors":"Andrew Gow","doi":"10.1089/aivt.2022.29028.editorial","DOIUrl":"https://doi.org/10.1089/aivt.2022.29028.editorial","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48255651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.1089/aivt.2022.29027.cfp
B. Lidbury
{"title":"Call for Special Issue Papers: Enhancing In Vitro Toxicology Through Machine Learning","authors":"B. Lidbury","doi":"10.1089/aivt.2022.29027.cfp","DOIUrl":"https://doi.org/10.1089/aivt.2022.29027.cfp","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46286201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. M. Syed, V. Chaudhari, U. Murty, Bidya Dhar Sahu, Subhamoy Banerjee
{"title":"Nootkatone Essential Oil-Encapsulated Lipid Nanoparticles Interaction with Rat Cardiomyoblast (H9c2) Cells","authors":"A. M. Syed, V. Chaudhari, U. Murty, Bidya Dhar Sahu, Subhamoy Banerjee","doi":"10.1089/aivt.2021.0019","DOIUrl":"https://doi.org/10.1089/aivt.2021.0019","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46126604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fan Yu, K. Rudd, S. J. Pour, E. Trelles Sticken, O. Dethloff, R. Wieczorek, Thomas Nahde, L. Simms, F. Chapman, L. Czekala, M. Stevenson, G. O’Connell
{"title":"Preclinical Assessment of Tobacco-Free Nicotine Pouches Demonstrates Reduced In Vitro Toxicity Compared with Tobacco Snus and Combustible Cigarette Smoke","authors":"Fan Yu, K. Rudd, S. J. Pour, E. Trelles Sticken, O. Dethloff, R. Wieczorek, Thomas Nahde, L. Simms, F. Chapman, L. Czekala, M. Stevenson, G. O’Connell","doi":"10.1089/aivt.2021.0020","DOIUrl":"https://doi.org/10.1089/aivt.2021.0020","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44223872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.1089/aivt.2021.29025.edi
Andrew Gow
{"title":"Multiple Approaches to Understanding the Toxic Dose","authors":"Andrew Gow","doi":"10.1089/aivt.2021.29025.edi","DOIUrl":"https://doi.org/10.1089/aivt.2021.29025.edi","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46569058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bincy A John, David J Sloan, Timothy C Jensen, Sreenivasa C Ramaiahgari, Peter End, Gabrielle E Resh, Randall E McClelland
Introduction: Because of the importance to create in vitro screening tools that better mimic in vivo models, for exposure responses to drugs or toxicants, reproducible and adaptable culture platforms must evolve as approaches to replicate functions that are native to human organ systems. The Stairstep Waterfall (SsWaterfall) Fluidic Culture System is a unidirectional, multiwell, gravity-driven, cell culture system with micro-channels connecting 12 wells in each row (8-row replicates). Materials and Methods: The construct allows for the one-way flow of medium, parent and metabolite compounds, and the cellular signaling between connected culture wells while simultaneously operating as a cascading flow and discretized nonlinear dosing device. Initial cell seeding in SsWaterfall mimics traditional static plate protocols but thereafter functions with controlled flow and ramping concentration versus time exposure environments. Results: To investigate the utility of a microfluidic system for predicting drug efficacy and toxicity, we first delineate device design, fabrication, and characterization of a disposable dosing and gradient-exposure platform. We start with detailed characterizations by demarcating various features of the device, including low nonspecific binding, wettability, biocompatibility with multiple cell types, intra-well and inter-well flow, and efficient auto-mixing properties of dose compounds added into the platform. Discussion: We demonstrate the device utility using an example in sequential testing-screening drug toxicity and efficacy across wide-ranging inducible exposures, 0 → IC100, featuring real-time assessments. Conclusion: The integrated auto-gradient technology, gravity flow with stairstep pathways, offers end-users an easy and quick alternative to evaluate broad-ranging toxicity of new compound entities (e.g., pharmaceutical, environmental, agricultural, cosmetic) as opposed to traditional/arduous manual drug dilutions and/or expensive robotic technology.
{"title":"A Unidirectional 96-Well Fluidic Culture Platform for Upstream Cell Dosing with Subsequent Downstream Nonlinear and Ascending Exposure Gradients for Real-Time and Cell-Based Toxicity Screening Environments.","authors":"Bincy A John, David J Sloan, Timothy C Jensen, Sreenivasa C Ramaiahgari, Peter End, Gabrielle E Resh, Randall E McClelland","doi":"10.1089/aivt.2021.0006","DOIUrl":"https://doi.org/10.1089/aivt.2021.0006","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Because of the importance to create <i>in vitro</i> screening tools that better mimic <i>in vivo</i> models, for exposure responses to drugs or toxicants, reproducible and adaptable culture platforms must evolve as approaches to replicate functions that are native to human organ systems. The Stairstep Waterfall (SsWaterfall) Fluidic Culture System is a unidirectional, multiwell, gravity-driven, cell culture system with micro-channels connecting 12 wells in each row (8-row replicates). <b><i>Materials and Methods:</i></b> The construct allows for the one-way flow of medium, parent and metabolite compounds, and the cellular signaling between connected culture wells while simultaneously operating as a cascading flow and discretized nonlinear dosing device. Initial cell seeding in SsWaterfall mimics traditional static plate protocols but thereafter functions with controlled flow and ramping concentration versus time exposure environments. <b><i>Results:</i></b> To investigate the utility of a microfluidic system for predicting drug efficacy and toxicity, we first delineate device design, fabrication, and characterization of a disposable dosing and gradient-exposure platform. We start with detailed characterizations by demarcating various features of the device, including low nonspecific binding, wettability, biocompatibility with multiple cell types, intra-well and inter-well flow, and efficient auto-mixing properties of dose compounds added into the platform. <b><i>Discussion:</i></b> We demonstrate the device utility using an example in sequential testing-screening drug toxicity and efficacy across wide-ranging inducible exposures, 0 → IC100, featuring real-time assessments. <b><i>Conclusion:</i></b> The integrated auto-gradient technology, gravity flow with stairstep pathways, offers end-users an easy and quick alternative to evaluate broad-ranging toxicity of new compound entities (e.g., pharmaceutical, environmental, agricultural, cosmetic) as opposed to traditional/arduous manual drug dilutions and/or expensive robotic technology.</p>","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"7 4","pages":"175-191"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743950/pdf/aivt.2021.0006.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10510301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Krieger, T. Auernhammer, J. Hotchkiss, Daniel M. Wilson
{"title":"Mechanistic Profiling of Inhalation Sensory Irritants with a Computational Model for Transient Receptor Potential Vanilloid Subfamily Type 1","authors":"S. Krieger, T. Auernhammer, J. Hotchkiss, Daniel M. Wilson","doi":"10.1089/aivt.2021.0007","DOIUrl":"https://doi.org/10.1089/aivt.2021.0007","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42495507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Shokrzadeh, P. Mortazavi, Atefeh Moghadami, B. Khayambashi, F. Motafeghi
{"title":"Synergistic Antiproliferative and Anticancer Activity of Carotenoid Lutein or Coenzyme Q10 in Combination with Doxorubicin on the MCF7 Cell Line","authors":"M. Shokrzadeh, P. Mortazavi, Atefeh Moghadami, B. Khayambashi, F. Motafeghi","doi":"10.1089/aivt.2021.0008","DOIUrl":"https://doi.org/10.1089/aivt.2021.0008","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49386520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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