{"title":"Usefulness and Applicability of Integrated Strategy Approaches in Toxicology","authors":"C. Eskes, S. Casati, N. Kleinstreuer, J. Ezendam","doi":"10.1089/aivt.2021.0021","DOIUrl":"https://doi.org/10.1089/aivt.2021.0021","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45098581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Kojima, T. Nakada, A. Yagami, H. Todo, Jihei Nishimura, M. Yagi, Mariko Sugiyama, Keiko Yamamoto, Y. Ikarashi, H. Sakaguchi, M. Yamaguchi, Morihiko Hirota, Hidefumi Ikeda, Noriyasu Imai, M. Hatao
Introduction: Animal tests of cosmetic ingredients and products have been banned in the EU since 2013. However, in Japan, the application of new quasi-drugs requires the generation of 24-hour data ...
{"title":"A Step-by-Step Approach for Assessing Human Skin Irritation Without Animal Testing for Quasi-Drugs and Cosmetic Products","authors":"H. Kojima, T. Nakada, A. Yagami, H. Todo, Jihei Nishimura, M. Yagi, Mariko Sugiyama, Keiko Yamamoto, Y. Ikarashi, H. Sakaguchi, M. Yamaguchi, Morihiko Hirota, Hidefumi Ikeda, Noriyasu Imai, M. Hatao","doi":"10.1089/aivt.2021.0016","DOIUrl":"https://doi.org/10.1089/aivt.2021.0016","url":null,"abstract":"Introduction: Animal tests of cosmetic ingredients and products have been banned in the EU since 2013. However, in Japan, the application of new quasi-drugs requires the generation of 24-hour data ...","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42781219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Alépée, T. Abo, E. Adriaens, D. Bagley, K. Mewes
Introduction: Cosmetics Europe develops two defined approaches (DAs) for eye hazard identification, that is, addressing serious eye damage (Cat. 1), eye irritation (Cat. 2), and the absence thereof...
{"title":"Performance Statistics of Defined Approaches for Eye Hazard Identification of Non-Surfactant Liquids to Distinguish Between the Three United Nations Globally Harmonized System Categories","authors":"N. Alépée, T. Abo, E. Adriaens, D. Bagley, K. Mewes","doi":"10.1089/aivt.2021.0012","DOIUrl":"https://doi.org/10.1089/aivt.2021.0012","url":null,"abstract":"Introduction: Cosmetics Europe develops two defined approaches (DAs) for eye hazard identification, that is, addressing serious eye damage (Cat. 1), eye irritation (Cat. 2), and the absence thereof...","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41382097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01Epub Date: 2021-09-17DOI: 10.1089/aivt.2021.0009
Hedwig M Braakhuis, Fiona Murphy, Lan Ma-Hock, Susan Dekkers, Johannes Keller, Agnes G Oomen, Vicki Stone
Introduction: Here, we describe the generation of hypotheses for grouping nanoforms (NFs) after inhalation exposure and the tailored Integrated Approaches to Testing and Assessment (IATA) with which each specific hypothesis can be tested. This is part of a state-of-the-art framework to support the hypothesis-driven grouping and read-across of NFs, as developed by the EU-funded Horizon 2020 project GRACIOUS. Development of Grouping Hypotheses and IATA: Respirable NFs, depending on their physicochemical properties, may dissolve either in lung lining fluid or in acidic lysosomal fluid after uptake by cells. Alternatively, NFs may also persist in particulate form. Dissolution in the lung is, therefore, a decisive factor for the toxicokinetics of NFs. This has led to the development of four hypotheses, broadly grouping NFs as instantaneous, quickly, gradually, and very slowly dissolving NFs. For instantaneously dissolving NFs, hazard information can be derived by read-across from the ions. For quickly dissolving particles, as accumulation of particles is not expected, ion toxicity will drive the toxic profile. However, the particle aspect influences the location of the ion release. For gradually dissolving and very slowly dissolving NFs, particle-driven toxicity is of concern. These NFs may be grouped by their reactivity and inflammation potency. The hypotheses are substantiated by a tailored IATA, which describes the minimum information and laboratory assessments of NFs under investigation required to justify grouping. Conclusion: The GRACIOUS hypotheses and tailored IATA for respiratory toxicity of inhaled NFs can be used to support decision making regarding Safe(r)-by-Design product development or adoption of precautionary measures to mitigate potential risks. It can also be used to support read-across of adverse effects such as pulmonary inflammation and subsequent downstream effects such as lung fibrosis and lung tumor formation after long-term exposure.
{"title":"An Integrated Approach to Testing and Assessment to Support Grouping and Read-Across of Nanomaterials After Inhalation Exposure.","authors":"Hedwig M Braakhuis, Fiona Murphy, Lan Ma-Hock, Susan Dekkers, Johannes Keller, Agnes G Oomen, Vicki Stone","doi":"10.1089/aivt.2021.0009","DOIUrl":"https://doi.org/10.1089/aivt.2021.0009","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Here, we describe the generation of hypotheses for grouping nanoforms (NFs) after inhalation exposure and the tailored Integrated Approaches to Testing and Assessment (IATA) with which each specific hypothesis can be tested. This is part of a state-of-the-art framework to support the hypothesis-driven grouping and read-across of NFs, as developed by the EU-funded Horizon 2020 project GRACIOUS. <b><i>Development of Grouping Hypotheses and IATA:</i></b> Respirable NFs, depending on their physicochemical properties, may dissolve either in lung lining fluid or in acidic lysosomal fluid after uptake by cells. Alternatively, NFs may also persist in particulate form. Dissolution in the lung is, therefore, a decisive factor for the toxicokinetics of NFs. This has led to the development of four hypotheses, broadly grouping NFs as instantaneous, quickly, gradually, and very slowly dissolving NFs. For instantaneously dissolving NFs, hazard information can be derived by read-across from the ions. For quickly dissolving particles, as accumulation of particles is not expected, ion toxicity will drive the toxic profile. However, the particle aspect influences the location of the ion release. For gradually dissolving and very slowly dissolving NFs, particle-driven toxicity is of concern. These NFs may be grouped by their reactivity and inflammation potency. The hypotheses are substantiated by a tailored IATA, which describes the minimum information and laboratory assessments of NFs under investigation required to justify grouping. <b><i>Conclusion:</i></b> The GRACIOUS hypotheses and tailored IATA for respiratory toxicity of inhaled NFs can be used to support decision making regarding Safe(r)-by-Design product development or adoption of precautionary measures to mitigate potential risks. It can also be used to support read-across of adverse effects such as pulmonary inflammation and subsequent downstream effects such as lung fibrosis and lung tumor formation after long-term exposure.</p>","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"7 3","pages":"112-128"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39598503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Through repeated and sustained occupational, industrial, and environmental sources, arsenic exposure is a world-wide health problem that affects 200 million people resulting in skin lesions, diabet...
{"title":"Using In Vitro Models to Dissect the Molecular Effects of Arsenic Exposure in Skin and Lung Cell Lines","authors":"Christine Kim, B. Ceresa","doi":"10.1089/aivt.2020.0026","DOIUrl":"https://doi.org/10.1089/aivt.2020.0026","url":null,"abstract":"Through repeated and sustained occupational, industrial, and environmental sources, arsenic exposure is a world-wide health problem that affects 200 million people resulting in skin lesions, diabet...","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44198011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anne A. Adeyanju, T. Oyedeji, Wonderful B. Adebagbo, Oluwamuyiwa P. Oluyitan, Sylvester A. Adeyanju
Introduction: Whole blood undergoes oxidative stress that precipitates biochemical changes that can lead to lesions during storage. This study attempts to evaluate the effectiveness of gallic acid ...
{"title":"Protective Effects of Luteolin and Gallic Acid Against Storage-Induced Oxidative Damage in Whole Blood of Female Wistar Rats","authors":"Anne A. Adeyanju, T. Oyedeji, Wonderful B. Adebagbo, Oluwamuyiwa P. Oluyitan, Sylvester A. Adeyanju","doi":"10.1089/aivt.2020.0028","DOIUrl":"https://doi.org/10.1089/aivt.2020.0028","url":null,"abstract":"Introduction: Whole blood undergoes oxidative stress that precipitates biochemical changes that can lead to lesions during storage. This study attempts to evaluate the effectiveness of gallic acid ...","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44093793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marie McGee Hargrove, B. Parr-Dobrzanski, Lei Li, S. Constant, JoAnn Wallace, P. Hinderliter, D. Wolf, A. Charlton
Introduction: The United States Environmental Protection Agency (US EPA) has issued a directive to reduce mammalian studies to 30% by 2025. The application of new approach methodologies is being im...
{"title":"Use of the MucilAir Airway Assay, a New Approach Methodology, for Evaluating the Safety and Inhalation Risk of Agrochemicals","authors":"Marie McGee Hargrove, B. Parr-Dobrzanski, Lei Li, S. Constant, JoAnn Wallace, P. Hinderliter, D. Wolf, A. Charlton","doi":"10.1089/aivt.2021.0005","DOIUrl":"https://doi.org/10.1089/aivt.2021.0005","url":null,"abstract":"Introduction: The United States Environmental Protection Agency (US EPA) has issued a directive to reduce mammalian studies to 30% by 2025. The application of new approach methodologies is being im...","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44696319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"When and How Can We Stop Using Animals in Toxicology?","authors":"Andrew Gow, E. Corsini","doi":"10.1089/aivt.2021.0014","DOIUrl":"https://doi.org/10.1089/aivt.2021.0014","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44604200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kristen A. Hopperstad, Theresa Truschel, Tom Wahlicht, W. Stewart, A. Eicher, T. May, C. Deisenroth
Introduction Investigation of normal human thyroid function using in vitro culture systems is dependent on cells that recapitulate physiology of differentiated thyrocytes. Primary thyrocytes retain features of the native organ but have limited lifespan in culture. Immortalized thyrocytes offer an alternative if challenges maintaining phenotypic stability can be overcome to retain functional features of primary cells. Materials and Methods CI-SCREEN immortalization technology was applied to normal human thyroid tissue to generate four cell line variants. The lines were characterized for transgene integration, biomarker expression, genomic stability, and proliferation rates. Thyroid Stimulating Hormone (TSH)-dependent morphology, thyroglobulin production, thyroxine hormone synthesis, and viability were assessed using conventional 2D monolayer and 3D microtissue culture formats in huThyrEC or h7H medium. Results Despite differential transgene profiles, the lines had similar biomarker expression patterns and proliferation rates. In 2D culture there was no thyroxine synthesis or changes in viability, but TSH-dependent thyroglobulin production was more significant for several lines in h7H than huThyrEC medium. Comparatively, in 3D microtissues, TSH-dependent thyroglobulin induction was greater for cell lines in h7H medium. Synthesis of thyroxine in one cell line was higher than background with TSH exposure, but not significantly different than control. Discussion Immortalization of primary human thyrocytes yielded transgenic lines of epithelial origin. When evaluated in 2D or 3D culture formats, h7H medium supported thyroglobulin production to a greater magnitude than huThyrEC medium. One cell line cultured in 3D microtissue format marginally recapitulated T4 synthesis under continuous TSH exposure. Conclusion Select human thyroid cell lines exhibited morphological and functional features of primary thyrocytes and are a novel resource for in vitro disease modeling and toxicity testing that will enable reproducible culture models more representative of normal human thyroid function.
{"title":"Characterization of Novel Human Immortalized Thyroid Follicular Epithelial Cell Lines.","authors":"Kristen A. Hopperstad, Theresa Truschel, Tom Wahlicht, W. Stewart, A. Eicher, T. May, C. Deisenroth","doi":"10.1089/AIVT.2020.0027","DOIUrl":"https://doi.org/10.1089/AIVT.2020.0027","url":null,"abstract":"Introduction\u0000Investigation of normal human thyroid function using in vitro culture systems is dependent on cells that recapitulate physiology of differentiated thyrocytes. Primary thyrocytes retain features of the native organ but have limited lifespan in culture. Immortalized thyrocytes offer an alternative if challenges maintaining phenotypic stability can be overcome to retain functional features of primary cells.\u0000\u0000\u0000Materials and Methods\u0000CI-SCREEN immortalization technology was applied to normal human thyroid tissue to generate four cell line variants. The lines were characterized for transgene integration, biomarker expression, genomic stability, and proliferation rates. Thyroid Stimulating Hormone (TSH)-dependent morphology, thyroglobulin production, thyroxine hormone synthesis, and viability were assessed using conventional 2D monolayer and 3D microtissue culture formats in huThyrEC or h7H medium.\u0000\u0000\u0000Results\u0000Despite differential transgene profiles, the lines had similar biomarker expression patterns and proliferation rates. In 2D culture there was no thyroxine synthesis or changes in viability, but TSH-dependent thyroglobulin production was more significant for several lines in h7H than huThyrEC medium. Comparatively, in 3D microtissues, TSH-dependent thyroglobulin induction was greater for cell lines in h7H medium. Synthesis of thyroxine in one cell line was higher than background with TSH exposure, but not significantly different than control.\u0000\u0000\u0000Discussion\u0000Immortalization of primary human thyrocytes yielded transgenic lines of epithelial origin. When evaluated in 2D or 3D culture formats, h7H medium supported thyroglobulin production to a greater magnitude than huThyrEC medium. One cell line cultured in 3D microtissue format marginally recapitulated T4 synthesis under continuous TSH exposure.\u0000\u0000\u0000Conclusion\u0000Select human thyroid cell lines exhibited morphological and functional features of primary thyrocytes and are a novel resource for in vitro disease modeling and toxicity testing that will enable reproducible culture models more representative of normal human thyroid function.","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"7 2 1","pages":"39-49"},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44892554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01Epub Date: 2021-03-17DOI: 10.1089/aivt.2019.0005.correx
[This corrects the article DOI: 10.1089/aivt.2019.0005.].
[这更正了文章DOI: 10.1089/ aivit .2019.0005.]。
{"title":"Correction to: <i>An Integrative Proteomic/Lipidomic Analysis of the Gold Nanoparticle Biocorona in Healthy and Obese Conditions</i>, by Kobos LM, et al. <i>Appl In Vitro Toxicol</i> 2019:5;150-166; DOI: 10.1089/aivt.2019.0005.","authors":"","doi":"10.1089/aivt.2019.0005.correx","DOIUrl":"https://doi.org/10.1089/aivt.2019.0005.correx","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1089/aivt.2019.0005.].</p>","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"7 1","pages":"34"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006583/pdf/aivt.2019.0005.correx.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25556218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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