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Entomology contribution in animal immunity: Determination of the crude thoraxial glandular protein extract of Stomoxys calcitrans as an antibody production enhancer in young horses 昆虫学在动物免疫中的贡献:测定粗胸腺蛋白提取物作为幼马抗体产生增强剂的作用
Q3 Agricultural and Biological Sciences Pub Date : 2017-12-31 DOI: 10.4081/jear.2017.7074
L. Rumokoy, S. Adiani, G. Assa, W. L. Toar, J. L. Abán
This experiment was conducted to evaluate the level of antigens protein contained in the crude thoraxial glandular protein (TGP) extract of Stomoxys calcitrans which function as immunity enhancer in young horses. The detection of protein content of the thoraxial glandular samples was performed by using a spectrophotometer Nano Drop-1000. This result showed that the lowest level of antigen protein was 0.54 mg/mL, the highest was 72 mg/mL, and the average was 0.675 mg/mL. Six foals were used and divided into two groups. The first group was treated with a solution of 100 μg of TGP by subcutaneous injection, the other group acted as control. The TGP extract was injected on the first day of the experiment. Three ml of blood were sampled from the jugular vein on the 14ᵗʰ day after TGP injection. The blood sampled was centrifuged and its serum placed in micro-tubes to observe the IgG level. The injection of TGP had a significant effect on the IgG level of the experiment animals (P<0.05). This experiment emphasized an important relation between entomology and animal husbandry; health improvement in the young animals was observed after the injection of the insect antigen, so it can be concluded that crude thoraxial glandular proteins of S. calcitrans can be used to improve the immunoglobulin-G circulation in foals.
本实验旨在评价具有免疫增强作用的口氧化钙的粗胸腺蛋白提取物中所含抗原蛋白的水平。使用分光光度计Nano Drop-1000对胸腺样品的蛋白质含量进行检测。该结果表明,抗原蛋白水平最低为0.54mg/mL,最高为72mg/mL,平均为0.675mg/mL。使用了六匹小马驹,并将其分为两组。第一组皮下注射TGP溶液100μg,另一组作为对照。在实验的第一天注射TGP提取物。从14ᵗʰTGP注射后第二天。将采集的血液离心,并将其血清放入微管中以观察IgG水平。注射TGP对实验动物IgG水平有显著影响(P<0.05),本实验强调了昆虫学与畜牧业的重要关系;注射昆虫抗原后,观察到幼兽的健康状况有所改善,因此可以得出结论,钙化转移链球菌的粗胸腺蛋白可以用于改善小马驹的免疫球蛋白-G循环。
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引用次数: 7
Trials on the Timing of Chemical Control of Lentil weevil, Bruchus lentis Frӧlich (Coleoptera: Chrysomelidae: Bruchinae) in Lentil Field in Gachsaran Region (Iran 伊朗Gachsaran地区扁豆田扁豆象甲Bruchus lentis Frõlich(鞘翅目:金蜂科:Bruchinae)化学防治时机的试验
Q3 Agricultural and Biological Sciences Pub Date : 2017-12-31 DOI: 10.4081/jear.2017.6829
K. Saeidi, S. Mirfakhraie
The lentil weevil, Bruchus lentis Frӧlich, (Coleoptera: Chrysomelidae: Bruchinae) is the most serious pest of lentil in Iran. Economic losses due to this pest reach up to 40% of the lentil crop. Over a two-year study (2012 and 2013) in Agricultural Research Station of Gachsaran Region, best timing of chemical control of B. lentis was determined. A field experiment with cultivation of lentil Sina variety Lens culinaris Medik was conducted in a randomized complete block design with five treatments and three replications. The treatments consisted of spraying four times (respectively, during the early flowering, 10 days after the first spraying, 10 days after the second spraying; 10 days after the third spraying) and control (without spraying). For the spraying from Endosulfan insecticide EC50% at ratio one liter per hectare was used. Three samples were taken from the pods and totally 150 pods from each replicate for contaminations of seeds were investigated. After the determination of the percent of seeds contamination, results were statistically analysed. Based on the results obtained, first spray treatment, with the mean contamination of 15.45% and second spray treatment with the mean contamination of 12.25% had the highest impact on reducing contamination lentil seeds to B. lentis and between them there was no statistically significant difference and were in one group. Therefore, spraying one time during the early flowering until 15 days after it was the best time to control of B. lentis.
扁豆象甲Bruchus lentis Frõlich(鞘翅目:金蜂科:Bruchinae)是伊朗最严重的扁豆害虫。这种害虫造成的经济损失高达扁豆作物的40%。在Gachsaran地区农业研究站进行的为期两年(2012年和2013年)的研究中,确定了对小扁豆进行化学防治的最佳时机。采用五个处理和三个重复的随机完全区组设计,对扁豆品种Lens culinaris Medik进行了田间试验。处理包括四次喷洒(分别在开花初期、第一次喷洒后10天、第二次喷洒后十天、第三次喷洒后的10天)和对照(不喷洒)。硫丹杀虫剂的喷洒使用EC50%,比例为每公顷1升。从这些豆荚中提取了三个样本,并对每个重复的豆荚中总共150个的种子污染进行了调查。在确定种子污染百分比后,对结果进行统计分析。根据获得的结果,第一次喷雾处理和第二次喷雾处理的平均污染率分别为15.45%和12.25%,对将污染扁豆种子减少为B.lents的影响最大,两者之间没有统计学上的显著差异,属于一组。因此,在早花期喷洒一次直到15天后是控制扁豆的最佳时间。
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引用次数: 1
Eurycnemus v. d. Wulp (Diptera, Chironomidae) newly recorded in China 中国新记录的Eurycnemus v.d.Wulp(直翅目、摇蚊科)
Q3 Agricultural and Biological Sciences Pub Date : 2017-12-01 DOI: 10.4081/JEAR.2017.7129
L. Li, H. Tang
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引用次数: 0
Comparison of cockroach fungal contamination in the clinical and non-clinical environments from Iran 伊朗临床和非临床环境中蟑螂真菌污染的比较
Q3 Agricultural and Biological Sciences Pub Date : 2017-09-28 DOI: 10.4081/JEAR.2017.6758
B. Davari, A. Hassanvand, H. Nasirian, S. A. Ghiasian, A. Salehzadeh, M. Nazari
Cockroaches have been associated with human environments threatening human health. A cross-sectional study about cockroach fungal contamination in the hospital, restaurant and household environments from Khorramabad, Lorestan province of Iran between August 2015 and March 2016 was done. Sampling was uniformly carried out monthly from the randomly selected sites. Fungi were isolated from the external surfaces of cockroaches using standard method. Periplaneta americana (66.7%) was found the most infested cockroach, less percentage were observed in Blattella germanica (18.6%) and Blatta orientalis (14.7%). Hospitals (66.7%) were found the most infested places in comparison with households (18.6%) and restaurants (14.7%). Households (64.3%) were found the most cockroach fungal contaminated places than hospitals (49.0%) and restaurants (59.1%). The highest and the lowest infestation of cockroaches were observed in January (30.0%) and March (7.0%) respectively, while the highest and the lowest cockroach fungal contamination were observed in November (73.3%) and March (14.3%). B. germanica (60.7%) was the most fungal contaminated cockroach. Candida , Geotrichum and Penicillium were the most frequent cockroach fungal contaminating genera. Statistical analysis revealed significant differences between cockroach infestation places (P=0.022), locality (P=0.03), and monthly (P=0.0001) cockroach fungal contamination, respectively. As a conclusion, the highest cockroach fungal contamination was observed in B. germanica, followed by P. americana and B. orientalis. Some human fungal opportunistic pathogens were also found among the fungal contaminated cockroaches. Recent epidemiological survey showed that the mortality rates of the infective diseases were increased, indicating cockroaches involved in their transferring.
蟑螂与威胁人类健康的人类环境有关。2015年8月至2016年3月,对伊朗洛雷斯坦省Khorramabad的医院、餐厅和家庭环境中的蟑螂真菌污染进行了横断面研究。每月从随机选择的地点统一取样。使用标准方法从蟑螂的外表面分离出真菌。美洲大蠊(66.7%)是最常见的蟑螂,德国小蠊(18.6%)和东方大蠊(14.7%1月(30.0%)和3月(7.0%),11月(73.3%)和3月份(14.3%)蟑螂真菌污染最高和最低。德国小蠊(60.7%)是真菌污染最严重的蟑螂。念珠菌属、毛霉菌属和青霉属是蟑螂最常见的真菌污染属。统计分析显示,蟑螂侵扰地点(P=0.022)、地点(P=0.03)和每月(P=0.001)蟑螂真菌污染之间分别存在显著差异。因此,蟑螂真菌污染最高的是德国小蠊,其次是美洲大蠊和东方小蠊。在受真菌污染的蟑螂中也发现了一些人类真菌机会性病原体。最近的流行病学调查显示,感染性疾病的死亡率增加,表明蟑螂参与了它们的转移。
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引用次数: 19
First case of traumatic myiasis caused by Calliphora vicina in a crested porcupine Hystrix cristata L. in Italy 意大利冠状豪猪刺蝇致创伤性蝇蛆病首例报道
Q3 Agricultural and Biological Sciences Pub Date : 2017-09-11 DOI: 10.4081/JEAR.2017.6823
D. Scaravelli, C. Senini, T. Bonacci
Calliphora vicina (Diptera: Calliphoridae) it is a facultative ectoparasite responsible for traumatic myiasis in humans and warmblooded vertebrates in the world. In this work one case of traumatic myasis caused by C. vicina (Diptera Calliphoirdae) is reported for the first time in a vulnerable crested porcupine ( Hystrix cristata Linnaeus, 1758). A total of 30 larvae located in the posterior-dorsal area of the animal were removed from inside the lesion and either preserved in ethanol or reared to the adult stage. This report shows the great ability of C. vicina to use many organic matter for the food source’s offspring, including many live vertebrates.
丽蝇(直翅目:丽蝇科)是一种兼性外寄生虫,在世界范围内对人类和温血脊椎动物的创伤性蝇蛆病负责。在这项工作中,首次报道了一例由C.vicina引起的创伤性肌病(直翅目剑尾虫科)发生在一只脆弱的冠豪猪身上(Hystrix crista Linnaeus,1758)。将位于动物背部后部区域的总共30只幼虫从病变内取出,保存在乙醇中或饲养至成年期。这份报告显示了C.vicina为食物来源的后代(包括许多活脊椎动物)利用许多有机物的强大能力。
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引用次数: 5
Damage caused by Tetranychus Merganser Bondreaux (Acari: Tetranychidae) on Nopal Verdura Opuntia Ficus-Indica (L.) Miller during winter 秋沙叶螨(蜱螨亚纲:叶螨科)对印度无花果的危害冬天的米勒
Q3 Agricultural and Biological Sciences Pub Date : 2017-09-11 DOI: 10.4081/jear.2017.6281
J. Lima-Espíndola, J. M. Vanegas-Rico
The spider mite Tetranychus merganser occurs in some crops in Mexico and USA. This mite could be considered a potential pest in countries like Japan. In this work we recorded the damages that T. merganser causes to the nopal verdura (prickly pear vegetable crop) in one of the principal cactus crop regions of Mexico.
在墨西哥和美国的一些作物中都会出现这种蜘蛛螨——秋沙叶螨。这种螨在日本等国可能被认为是一种潜在的害虫。在这项工作中,我们记录了秋沙鸭对墨西哥主要仙人掌作物地区之一的刺梨蔬菜造成的损害。
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引用次数: 3
New insights on Lepidoptera of Southern Italy with description of the male of Coenotephria antonii Hausmann 2011 (Lepidoptera) 意大利南部鳞翅目的新认识及2011(鳞翅目)Coenotephria antonii Hausmann雄性记述
Q3 Agricultural and Biological Sciences Pub Date : 2017-09-11 DOI: 10.4081/JEAR.2017.6783
M. Infusino, G. Luzzi, S. Scalercio
Southern Italy is of particular biogeographic interest due to the location at the center of the Mediterranean Basin and its great environmental heterogeneity. Despite the faunistic interest of this territory, many insect taxa are still little investigated. Among insects, Lepidoptera have a relatively well known fauna, significantly increased in recent years, but there are still some gaps of knowledge in several habitats. The aim of this work was to contribute to the knowledge of the Macrolepidoptera of Southern Italy, focusing our study in Calabria, and to offer some thoughts on the role played by the Mediterranean mountain forests for the biodiversity conservation. Samplings were carried out in three mountainous areas of Calabria (Pollino Massif, Sila Massif and Serre Mountains) in May-November 2015 and in April-November 2016, using UV-LED light traps. We found ten species of high faunistic interest. Three species, Nebula senectaria , Perizoma lugdunaria and Acasis appensata , were for the first time recorded from Southern Italy, while seven were for the first time recorded from Calabria: Coenotephria antonii, Thera obeliscata, Triphosa dubitata, Trichopteryx carpinata, Asteroscopus sphinx, Lithophane semibrunnea and Sideridis reticulata . Of great interest was the discovery of the first male certainly attributable to Coenotephria antonii, endemic of Southern Italy, here described for the first time. The results exposed confirm that the fauna of Southern Italy is of great conservation value, hosting endemisms and several relict populations of European and Asiatic species with differentiated genetic lineages highly vulnerable to the climate change expected for the coming decades.
意大利南部位于地中海盆地中心,环境异质性强,因此具有特别的生物地理学意义。尽管该地区具有动物学意义,但许多昆虫分类群仍然很少被研究。在昆虫中,鳞翅目有一个相对知名的动物群,近年来显著增加,但在一些栖息地仍存在一些知识空白。这项工作的目的是促进对意大利南部大型鳞翅目的了解,重点是我们在卡拉布里亚的研究,并就地中海山林在生物多样性保护中所起的作用提供一些想法。2015年5月至11月和2016年4月至11日,使用UV-LED光阱在卡拉布里亚的三个山区(Pollino Massif、Sila Massif和Serre Mountains)进行了采样。我们发现了十个动物区系高度感兴趣的物种。三个物种,Nebula senectaria、Perizoma lugdunaria和Acasis appenseta,是首次在意大利南部记录到的,而七个物种是首次在卡拉布里亚记录到的:Antoni Coenotephra、Thera obeliscata、Triphosa dubitata、Trichopteryx carpinata、Asteroscopus sphinx、Lithophane semibrunnea和Sideridis reticulata。令人感兴趣的是,发现了第一只雄性,肯定是意大利南部特有的安东尼氏新冠病毒,这是这里首次描述的。暴露的结果证实,意大利南部的动物群具有巨大的保护价值,拥有欧洲和亚洲物种的特有种和几个残余种群,这些物种具有分化的遗传谱系,极易受到未来几十年气候变化的影响。
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引用次数: 4
To what extent does salt (NaCl) affect Anopheles gambiae sensu lato mosquito larvae survival? 盐(NaCl)在多大程度上影响冈比亚按蚊幼虫的存活?
Q3 Agricultural and Biological Sciences Pub Date : 2017-09-11 DOI: 10.4081/JEAR.2017.6594
Nzira Lukwa, T. Mduluza, C. Nyoni, M. Zimba
The effect of salt (NaCl) on Anopheles gambiae sensu lato (s.l.) mosquito larval breeding was ascertained under laboratory conditions. No larval mortality occurred when the Cl– concentrations were between 0.017 ppt (0.03 ppt salinity) and 7.371 ppt (13.25 ppt salinity). However, 9%, 24%, 73.5%, 91.5% and 99.5% larval mortality occurred at 10.828 ppt (19.49 ppt salinity), 16.069 ppt (28.95 ppt salinity), 18.739 ppt (33.77 ppt salinity), 32.587 ppt (58.82 ppt salinity) and 47.326 ppt (85.37 ppt salinity) NaCl concentrations respectively. The lower NaCl concentrations resulting in LC50, (lethal concentration for 50% larval mortality), LC90 (lethal concentration for 90% larval mortality), LC95 (lethal concentration for 95% larval mortality), and LC99 (lethal concentration for 99% larval mortality) were 23.12 ppt (41.19 ppt salinity), 24.91 ppt (44.42 ppt salinity), 27.76 ppt (49.56 ppt salinity) and 33.87 ppt (60.568 ppt salinity) respectively. The upper NaCl concentration resulting in LC50, LC90, LC95 and LC99 were 32.89 ppt (58.83 ppt salinity), 37.21 ppt (66.63 ppt salinity), 44.79 ppt (80.32 ppt salinity) and 63.76 ppt (114.55 ppt salinity) respectively. In conclusion, the level of water salinity may indicate the presence or absence of An. gambiae s.l. mosquito larvae and this information can be used for disease control purposes.
在实验室条件下,确定了盐(NaCl)对冈比亚按蚊幼虫繁殖的影响。当Cl–浓度在0.017 ppt(0.03 ppt盐度)和7.371 ppt(13.25 ppt咸度)之间时,没有幼虫死亡。然而,在10.828 ppt(19.49 ppt盐度)、16.069 ppt(28.95 ppt矿化度)、18.739 ppt(33.77 ppt矿化率)、32.587 ppt(58.82 ppt盐度率)和47.326 ppt(85.37 ppt盐度度)的NaCl浓度下,幼虫死亡率分别为9%、24%、73.5%、91.5%和99.5%。导致LC50(50%幼虫死亡率的致死浓度)、LC90(90%幼虫死亡率的致命浓度,27.76ppt(盐度49.56ppt)和33.87ppt(矿化度60.568ppt)。导致LC50、LC90、LC95和LC99的较高NaCl浓度分别为32.89 ppt(58.83 ppt盐度)、37.21 ppt(66.63 ppt盐度。总之,水的盐度水平可能表明冈比亚蚊幼虫的存在或不存在,这些信息可用于疾病控制目的。
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引用次数: 2
Boreoheptagyia ortladamellica sp. nov. (Diptera, Chironomidae) from Italian Alps 意大利阿尔卑斯山区的北凤蝶属(双翅目,手蛾科)
Q3 Agricultural and Biological Sciences Pub Date : 2017-09-11 DOI: 10.4081/JEAR.2017.6860
B. Rossaro
A new species from Italian Alps, Boreoheptagyia ortladamellica (Diptera, Chironomidae), is here described. The species is presently known only as adult male, it is similar to B. tibetica , because of the female-like antenna in adult male, but it can be separated by the following characters: much larger size, darker colour, medially pointed aedeagal lobe, knob-like and heavy sclerotized inferior volsella, gonostylus enlarged at basis with short spiniform setae at apex.
本文报道了意大利阿尔卑斯山区一新种——北凤蝶(双翅目,手蛾科)。该种目前只以成虫雄种而为人所知,由于成虫雄种的触角与雌虫相似,因此与藏红花属有一定的区别,但可以通过以下特征来区分:体型大得多,颜色深,叶尖向中尖,下叶结节状且硬化较重,基部形柄增大,先端具短刺状刚毛。
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引用次数: 2
Effects of various sugar feeding choices on survival and tolerance of honey bee workers to low temperatures 不同食糖选择对蜜蜂工人生存和耐低温性的影响
Q3 Agricultural and Biological Sciences Pub Date : 2017-04-21 DOI: 10.4081/JEAR.2017.6200
H. Abou-Shaara
Beekeepers usually supply their colonies with alternatives to nectar (i.e. sugar feeding) during dearth periods of the year, especially cold times of winter. The objective of the study was to determine the best substances to feed bees to enhance the tolerance and survival of honey bees ( Apis mellifera L.) to low temperatures. Seven feeding choices were compared under laboratory conditions. These feeding choices were: sugar syrup, liquid honey, creamed honey, honey candy, sugar candy, honey jelly, and honey/sugarcane juice jelly. The results showed that the number of bees attracted to each feeding choice was influenced significantly by feeding type. Worker bees were attracted to all feeding choices and showed a high preference to creamed honey, honey jelly or honey/juice jelly. The tolerance of honey bees to low temperature was enhanced when bees were fed on creamed honey, sugar syrup or honey candy. The mean time at which 50% of bees were able to survive ranged from 3 days (unfed bees) to 15.8 days (honey candy group). The survival rate of worker bees was highest when they fed on honey candy, creamed honey or sugar candy. In light of this study, creamed honey or honey candy can be considered the best feeding choices for bee colonies during winter to enhance their survival and tolerance to low temperatures.
养蜂人通常在一年中的缺水期,特别是冬季的寒冷时期,为蜂群提供花蜜的替代品(即食糖)。本研究的目的是确定喂养蜜蜂的最佳物质,以提高蜜蜂(Apis mellifera L.)对低温的耐受性和存活率。在实验室条件下比较了七种喂养选择。这些喂养选择是:糖浆、液态蜂蜜、奶油蜂蜜、蜂蜜糖、糖糖、蜂蜜果冻和蜂蜜/甘蔗汁果冻。结果表明,每种饲养方式对蜜蜂的数量有显著影响。工蜂被所有的喂养选择所吸引,并表现出对奶油蜂蜜、蜂蜜果冻或蜂蜜/果汁果冻的高度偏好。当蜜蜂食用奶油蜂蜜、糖浆或蜂蜜糖时,蜜蜂对低温的耐受性增强。50%的蜜蜂能够存活的平均时间从3天(未喂食的蜜蜂)到15.8天(蜂蜜糖组)不等。当工蜂以蜂蜜糖、奶油蜂蜜或糖为食时,它们的存活率最高。根据这项研究,奶油蜂蜜或蜂蜜糖可以被认为是蜂群在冬季的最佳喂养选择,以提高它们的生存能力和对低温的耐受性。
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引用次数: 26
期刊
Journal of Entomological and Acarological Research
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