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Genetic diversity of rice stem borer (Chilo suppressalis Walker) from Northern Iran and comparison with other countries 伊朗北部水稻螟虫的遗传多样性及其与其他国家的比较
Q3 Agricultural and Biological Sciences Pub Date : 2016-12-19 DOI: 10.4081/JEAR.2016.5507
M. Shayanmehr, E. Yoosefi-Lafooraki
Rice striped stem borer, Chilo suppressalis Walker (Lepidoptera: Crambidae) is considered the major pest of rice in Iran. Because of the serious damage on rice in Northern Iran, the present study was conducted to investigate genetic diversity within populations of C. suppressalis , from Mazandaran using a template of cytochrome oxidase I gene, 750 bps, ( COI ). Later the haplotypes from Iran were compared with those found in other countries. According to the results of this study, there is very low genetic diversity (two haplotypes) among different populations of this pest in populations of Northern Iran. The genetic similarity and low levels of genetic diversity of these populations suggest that the pest colonization occurred relatively recently and there is high gene flow between these populations of the province. In addition, haplotypes of Mazandaran province are different with those found in other countries. The similarity of Iranian population (Simorgh) with one population from China indicated that China might be the origin of C. suppresalis .
水稻条纹螟虫(鳞翅目:螟科)是伊朗水稻的主要害虫。针对伊朗北部水稻遭受的严重病害,利用细胞色素氧化酶I基因750 bps (COI)模板,对Mazandaran产的C. suppressalis群体的遗传多样性进行了研究。后来,伊朗的单倍型与其他国家的单倍型进行了比较。根据本研究的结果,在伊朗北部的不同种群中,这种害虫的遗传多样性很低(两个单倍型)。遗传相似性和低水平的遗传多样性表明,这些种群的定殖时间相对较晚,并且在该省这些种群之间存在较高的基因流动。此外,马赞达兰省的单倍型与其他国家的单倍型不同。伊朗种群(Simorgh)与中国一个种群的相似性表明中国可能是抑孢粉虱的起源地。
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引用次数: 2
Japanese Encephalitis vector abundance and infection frequency in Cuddalore District, Tamil Nadu, India: a five-year longitudinal study 印度泰米尔纳德邦Cuddalore地区日本脑炎媒介丰度和感染频率:一项为期五年的纵向研究
Q3 Agricultural and Biological Sciences Pub Date : 2016-12-19 DOI: 10.4081/JEAR.2016.5630
P. Samuel, D. Ramesh, V. Thenmozhi, J. Nagaraj, M. Muniaraj, N. Arunachalam
An entomological monitoring of Japanese encephalitis vectors from the Cuddalore district, Tamil Nadu was undertaken at biweekly intervals for 1 hr after dusk for five years to find out the abundance and JE virus activity longitudinally in three villages. A total of 95,644 vectors belonging to 31 species constituted predominantly by Culex vishnui subgroup and Culex gelidus 98.5%. JE virus was identified from Cx. tritaeniorhynchus (18), Cx. vishnui (1) and Cx. gelidus (6) giving infection rate of 0.482, 0.608 and 0.221 respectively. Abundance of Cx. tritaeniorhynchus and Cx. gelidus differed significantly by area, season and year (P<0.05) whereas Cx. vishuni differed significantly by season and year (P<0.05). Transmission was not observed throughout all the seasons and the infection rate was recorded maximum during Hot-Wet season 0.46 (confidence interval: 0.17-1.02). Culex tritaeniorhynchus dominated the catch and the Culex gelidus steadily increased in its abundance during this period compared to the earlier studies conducted from these areas which acts as a secondary vector along with the major vector, Cx. tritaeniorhynchus.
对泰米尔纳德邦Cuddalore地区的日本脑炎病媒进行了昆虫学监测,每两周一次,每次黄昏后1小时,持续5年,以便在3个村庄纵向查明乙脑病毒的丰度和活动性。蚊媒31种95,644只,以vishnui库蚊亚群为主,gelidus库蚊占98.5%。发现乙脑病毒来自Cx。三带喙(18),Cx。vishnui(1)和Cx。沙棘(6)感染率分别为0.482、0.608和0.221。Cx的丰度三带喙龙和Cx。不同地区、季节、年份间差异显著(P<0.05)。不同季节、年份间vishuni差异有统计学意义(P<0.05)。所有季节均未观察到传播,在湿热季节感染率最高,为0.46(置信区间:0.17-1.02)。三带喙库蚊占主导地位,与早期研究相比,在这一时期,吉力库蚊的数量稳步增加,与主要媒介Cx一起作为次要媒介。tritaeniorhynchus。
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引用次数: 3
Bio-control efficiency of Bacillus thuringiensis (Berliner) against the citrus leaf miner, Phyllocnistis citrella Stainton (Lep., Gracillariidae) under laboratory conditions 苏云金芽孢杆菌(Bacillus thuringiensis, Berliner)对柑桔叶螨的生物防效研究。在实验室条件下
Q3 Agricultural and Biological Sciences Pub Date : 2016-12-19 DOI: 10.4081/JEAR.2016.5403
K. Saeidi, E. Saeidi
The citrus leaf miner, Phyllocnistis citrella Stainton (Lep., Gracillariidae), is one of the most destructive pest of citrus and related Rutaceae and ornamental plants in Iran. Larvae damage leaves by creating serpentine feeding mines, which have been lead to reduce yield. Resistance and toxicity problems derived from synthetic insecticides have made it necessary to find more effective and healthier alternatives; therefore, bio-insecticides ( i.e ., Bacillus thuringiensis ) are becoming an important component in plant protection. The aim of the present study was to evaluate the efficiency of B. thuringiensis against P. citrella . Eight B. thuringiensis concentrations were used against P. citrella L3 on orange and mortality was recorded at 1, 4, 7 and 10 days after spraying. The results showed that B. thuringiensis significantly affected mortality of P. citrella . After 1, 4, 7 and 10 days of spraying 108 concentration of B. thuringiensis had significantly caused the highest mortality to the pest with 59.8, 68.4, 73.6 and 77.0%, respectively. Then the mortality percent decreased until it reached 6.5, 9.5, 39.3 and 46.7% at 101 concentration, respectively. In conclusion, the study indicated that B. thuringiensis is effective in controlling P. citrella under laboratory conditions.
柑橘叶矿工,Phyllocnistis citrella Stainton (Lep。是伊朗柑橘及其相关芜科和观赏植物最具破坏性的害虫之一。幼虫通过形成蛇形取食地雷来破坏叶片,导致产量下降。合成杀虫剂产生的抗药性和毒性问题使得必须寻找更有效和更健康的替代品;因此,生物杀虫剂(即苏云金芽孢杆菌)正成为植物保护的重要组成部分。本研究的目的是评价苏云金芽孢杆菌对柑橘假单胞菌的杀灭效果。用8种浓度的苏云金弧菌对柑桔上的柑橘小孢子虫L3进行喷施,分别于喷施后1、4、7、10 d记录死亡率。结果表明,苏云金芽孢杆菌对柑橘单胞菌的死亡率有显著影响。喷施1、4、7和10 d后,108浓度的苏云金螺旋藻对害虫的死亡率最高,分别为59.8%、68.4、73.6%和77.0%。在101浓度下,死亡率分别为6.5、9.5、39.3%和46.7%。综上所述,在实验室条件下,苏云金芽孢杆菌对柑橘假单胞菌有较好的控制作用。
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引用次数: 0
Spinnerets and silk-producing system of Segestria senoculata (Araneae, Araneomorphae, Segestriidae) 蛛丝蛛科蛛丝蛛形目蛛丝蛛科蛛丝蛛的吐丝器及产丝系统
Q3 Agricultural and Biological Sciences Pub Date : 2016-12-19 DOI: 10.4081/JEAR.2016.5934
S. Karschová, J. Hajer
The spinning apparatus and silk of Segestria senoculata were studied with the use of scanning electron microscopy and light microscopy, which confirmed the presence of four categories of spigots connected with four types of spinning glands ( i.e ., Glandulae ampullaceae major, Glandulae ampullaceae minor, Glandulae piriformes and Glandulae pseudaciniformes ). New data about the morphology of spinnerets and spigots were obtained for both the adults and nymphal stages of both sexes. For the first time the silken threads of retreats, signal threads and attachment discs of the members of Segestria were subjected to a detailed SEM study. The data resulting from studying the spinning apparatus of S. senoculata was compared to current knowledge of the silk producing systems of families belonging to the Dysderoidea superfamily. Silks that are emitted from spigots in the course of retreat construction are not (similarly to the other dysderoids) processed by the spider’s legs during the subsequent process of hardening. Apart from the major ampullate glands/spigots, segestriids also possess developed minor ampullate glands. Minor ampullate threads are used by S. senoculata spiders when making their signal threads.
利用扫描电镜和光镜对senoculata Segestria的纺丝器和丝进行了研究,证实了四类龙头与四种纺丝腺(即大壶状腺、小壶状腺、梨状腺和假腺)相连。获得了雌雄成虫和若虫期喷丝器和喷丝器形态的新数据。首次对隐窝的丝状线、信号线和附着盘进行了详细的扫描电镜研究。对senoculata的纺丝装置进行了研究,并与目前对Dysderoidea超科的产丝系统的了解进行了比较。蛛丝在缩回构造过程中从龙头排出,在随后的硬化过程中不会被蜘蛛的腿处理(类似于其他硬样)。除了主要壶形腺/龙头外,小壶形腺也发育良好。小壶腹蛛在制造信号线时使用壶腹蛛丝。
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引用次数: 2
Species composition of mosquitoes (Diptera: Culicidae) in Farashband district, southwest of Iran 伊朗西南部法拉什班德地区蚊种组成(双翅目:库蚊科)
Q3 Agricultural and Biological Sciences Pub Date : 2016-12-19 DOI: 10.4081/JEAR.2016.6268
Z. Soltani, D. Keshavarzi
Mosquitoes are potential vectors of some important diseases for humans and animals. Apart from mosquito borne diseases, mosquitoes cause nuisances for both humans and animals when they are abundant. The objective of this study was to determine the fauna of mosquitoes (Diptera: Culicidae) in Farashband County, southwest of Iran. The study was carried out from April to September 2012. Four collection sites, were selected randomly with regard to existing facilities in Farashband County. Sampling was carried out by dipping technique for collecting larvae and pyrethrum space spray collection (PSSC) for adult mosquitoes. Mean temperature was recorded for every month. A total of 1152 adults and 1505 larvae of Culicidae mosquitoes were collected, of which 3 genera and 7 species of Culicidae were recognized, namely, Anopheles superpictus , An. dthali, Culex sitaiticus, Cx. theileri, Cx. pipiens, Cx. tritaeniorhynchus and Culiseta longiareolata . Cs. longiareolata was the most frequent Culicidae mosquito collected at Farashband, with a total of 513, and 249 specimens, by larval and PSSC collection, respectively. The highest numbers of mosquitoes were collected in July (34.9%) and the lowest in April (3.8%), respectively. Regarding this research, there are some potential vectors in medical and veterinary importance in Farashband County and they are more active in July and June.
蚊子是人类和动物某些重要疾病的潜在传播媒介。除了蚊子传播的疾病外,蚊子数量多的时候也会给人类和动物带来麻烦。本研究的目的是确定伊朗西南部法拉希班德县蚊虫区系(双翅目:库蚊科)。该研究于2012年4月至9月进行。在法拉什班德县的现有设施中随机选择了四个收集点。采用浸渍法采集成蚊幼虫和除虫菊空间喷雾法采集成蚊。记录每个月的平均温度。共捕获库蚊科成蚊1152只,幼虫1505只,鉴定库蚊科超照按蚊3属7种。西地库蚊,c。theileri,残雪。侵害,残雪。三带喙库蚊和长喙库蚊。Cs。长毛卷腹蚊是疫区最常见的库蚊科蚊种,分别捕获幼虫513只和PSSC 249只。成蚊数量以7月最高(34.9%),4月最低(3.8%);本研究发现,法拉什班德县存在一些具有潜在医学和兽医意义的病媒生物,7、6月较为活跃。
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引用次数: 4
Nysius cymoides (Spinola) on Chenopodium quinoa Willd. cultivated in Italy 藜麦藜属植物上的棘蚜。栽培于意大利
Q3 Agricultural and Biological Sciences Pub Date : 2016-12-19 DOI: 10.4081/JEAR.2016.5897
S. Bocchi, D. Cinquanta, M. Negri, P. Dioli, L. Limonta
Quinoa ( Chenopodium quinoa Willd.) (Family: Amaranthaceae – APG classification) is an Andean grain recently introduced on the European market and cultivated in experimental fields. In one of these experimental fields, in San Giorgio Piacentino (Italy), a heavy bug infestation was observed. The species was identified as Nysius cymoides (Spinola) (Heteroptera Lygaeidae), a polyphagous species known as a pest of different crops. It occurs in the Mediterranean area from the sea level to the alpine meadows.
藜麦(Chenopodium Quinoa wild .)(科:苋科- APG分类)是最近引进欧洲市场并在试验田种植的安第斯谷物。在意大利圣乔治·皮亚琴蒂诺(San Giorgio Piacentino)的一个试验田中,观察到严重的虫害。经鉴定,该虫属多食性害虫,属异翅目Lygaeidae科。它发生在地中海地区,从海平面到高山草甸。
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引用次数: 8
Aggressive mosquito fauna and malaria transmission in a forest area targeted for the creation of an agro-industrial complex in the south of Cameroon 喀麦隆南部建立农工综合体目标林区的侵略性蚊子群和疟疾传播
Q3 Agricultural and Biological Sciences Pub Date : 2016-12-19 DOI: 10.4081/JEAR.2016.5930
P. N. Akono, C. Tonga, O. N. Hondt, M. Nsangou, R. Ngaha, G. T. Magne, L. Y. Enga, A. Y. Kayoum, P. N. Mache, L. A. Djomi, F. A. Mbouangouro, L. Lehman
Baseline entomological information should be collected before the implementation of industrial projects in malaria endemic areas. This allows for subsequent monitoring and evaluation of the project impact on malaria vectors. This study aimed at assessing the vectorial system and malaria transmission in two ecologically different villages of the South-Cameroon forest bloc targeted for the creation of an agro-industrial complex. For four consecutive seasons in 2013, adult mosquitoes were captured using Human Landing Catch in NDELLE village (located along a main road in a degraded forest with many fish ponds) and KOMBO village (located 5km far from the main road in a darker forest and crossed by the Mvobo River). Morpho-taxonomic techniques were used alongside molecular techniques for the identification of mosquito species. ELISA test was used for the detection of circumsporozoite protein antigen of Plasmodium falciparum . Mosquito biting rate was higher in NDELLE than in KOMBO (28.18 versus 17.34 bites per person per night). Mosquitoes had a strong tendency to endophagy both in NDELLE (73.57%) and KOMBO (70.21%). Three anophelines species were identified; An. gambiae , An. funestus s.s and An. moucheti s.s.. An. gambiae and An. funestus s.s. represented the bulk of aggressive mosquitoes in NDELLE (n=10,891; 96.62%). An. gambiae was responsible for 62.6% and 77.72% of malaria transmission in KOMBO and NDELLE respectively. Mean entomological inoculation rate recorded in KOMBO and NDELLE were 4.82 and 2.02 infective bites per person per night respectively. Vector control was mainly based on the use of long-lasting insecticidal nets and indoor residual spraying. The degraded forest environment added to the presence of fishponds resulted in the increase of aggressive mosquito density but not of malaria transmission. The managers should use these data for monitoring and evaluation of the impact of their project; malaria control strategies should be included in their project in order to mitigate the risk of increased malaria transmission as a result of the implementation of their projects.
在疟疾流行地区实施工业项目之前,应收集基本昆虫学资料。这样就可以对项目对疟疾病媒的影响进行后续监测和评价。这项研究旨在评估南喀麦隆森林集团中两个生态不同的村庄的病媒系统和疟疾传播,目标是建立一个农工综合体。在2013年连续四个季节,在NDELLE村(位于有许多鱼塘的退化森林中的一条主干道旁)和KOMBO村(位于距离主干道5公里的较暗森林中,Mvobo河穿过)使用人类着陆捕捞法捕获了成蚊。形态分类技术与分子技术共同用于蚊虫种类鉴定。采用ELISA法检测恶性疟原虫环孢子子蛋白抗原。NDELLE的蚊虫叮咬率高于KOMBO(28.18对17.34)。NDELLE(73.57%)和KOMBO(70.21%)的蚊类均有较强的自噬倾向。鉴定出按蚊3种;一个。冈比亚,安。funestus s.s和An。moucheti轮. .一个。冈比亚和安哥拉。以funestus s.s.为主要攻击蚊种(n=10,891);96.62%)。一个。冈比亚在KOMBO和NDELLE的疟疾传播率分别为62.6%和77.72%。KOMBO和NDELLE的昆虫学平均接种率分别为4.82和2.02个/人/夜。病媒控制主要基于使用长效杀虫蚊帐和室内滞留喷洒。森林环境的退化和鱼塘的存在导致侵略性蚊子密度的增加,但没有导致疟疾传播。管理人员应使用这些数据来监测和评价其项目的影响;应将疟疾控制战略纳入其项目,以减轻由于实施其项目而增加疟疾传播的风险。
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引用次数: 1
Physical characteristics and reproductive performance in Aedes (Diptera: Culicidae) 伊蚊的生理特征及繁殖表现(双翅目:库蚊科)
Q3 Agricultural and Biological Sciences Pub Date : 2016-12-19 DOI: 10.4081/JEAR.2016.5883
H. Dieng, F. Abang, A. H. Ahmad, I. Ghani, Tomomitsu Satho, F. Miake, H. Ahmad, W. F. Zuharah, A. Majid, R. Morales, N. P. Morales, C. N. Hipolito, G. Noweg
Body size is a physical factor of crucial importance underlying important traits of the reproductive dynamics of both sexes in mosquitoes. Most studies on the influence of body size in mating success of dengue vectors addressed sperm transfer to females and did not consider egg production, a prerequisite for population maintenance; male body size impact on reproduction has attracted little research interest with respect to sterile insect technique. In experiments involving differently sized adults, we examined whether the body size of the mates is a source of variation in reproductive outcome in Aedes aegypti . In the absence of male partners, large females (LF) showed better fecundity than small females (SF). In intraclass mating trials, egg production was much greater in largesized than smallsized pairs. There were comparable fecundities in large females mated with small males and large pairs. [SF•SM] and [SF•LM] pairs showed equivalent fecundity. Nonmating did not result in the production of viable eggs by either small or large females. We also observed that eggs produced by largesized females mated with small males had better hatching success than those from either small or large pairs. Mating between small females and large males resulted in poor egg viability.
体型是一个至关重要的物理因素,是蚊子两性繁殖动力学的重要特征。大多数关于体型对登革热媒介交配成功的影响的研究都涉及精子向雌性的转移,而没有考虑产卵,这是种群维持的先决条件;在昆虫不育技术方面,雄性体型对生殖的影响很少引起研究兴趣。在涉及不同体型的成年伊蚊的实验中,我们研究了配偶的体型是否是埃及伊蚊繁殖结果变化的一个来源。在没有雄性伴侣的情况下,体型较大的雌虫(LF)的繁殖力优于体型较小的雌虫(SF)。在类内交配试验中,大配对的产蛋量要比小配对大得多。大型雌性与小型雄性和大型配对交配的繁殖力相当。[SF•SM]和[SF•LM]对的繁殖力相当。不管是大雌还是小雌,不交配都不会产生可存活的卵。我们还观察到,大雌蝶与小雄蝶交配所产蛋的孵化成功率高于小雌蝶和大雌蝶。体型小的雌性和体型大的雄性交配导致卵子存活率低。
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引用次数: 7
Effect of Citrullus colocynthis L. (Cucurbitaceae) agglutinin on gene expression of caspases in Ectomyelois ceratoniae Zeller (Lepidoptera: Crambidae) 瓜类凝集素对角裂叶蝉半胱天冬酶基因表达的影响
Q3 Agricultural and Biological Sciences Pub Date : 2016-12-19 DOI: 10.4081/JEAR.2016.5699
S. Ramzi, A. Sahragard, Jalal Jalali Sendi, A. Aalami
The larvae of Ectomyelois ceratoniae Zeller were reared on the artificial diets containing 2% of Citrullus colocynthis L. (Cucurbitaceae) lectin and control to find any changes in gene expression of caspases. Specific primers were designed to amplify genes of six found caspases including Caspase 1, 2, 3, 4, 5 and 6. RNA of both treatments were extracted and cDNA molecules were synthesized prior to gene expression. Results of quantitative real-time polymerase chain reaction revealed that expression of all caspases in the fed larvae by 2% of lectin increased in comparison with control except for caspase 1 as 2.99, 5.7, 17.13, 23.18, 10.44-fold. These results are consistent with our previous studies on the lower activities of digestive enzymes that are due to death of midgut cells followed by binding of lectin molecule.
将ceratoniae ectomyelis Zeller幼虫分别饲喂添加2%瓜科(Citrullus colocynthis L.)凝集素和对照的人工饲料,观察半胱天冬酶基因表达的变化。设计特异性引物扩增已发现的6种Caspase,包括Caspase 1、2、3、4、5和6。在基因表达前提取两种处理的RNA并合成cDNA分子。实时荧光定量聚合酶链反应结果显示,添加2%凝集素后,除caspase 1表达量为对照的2.99倍、5.7倍、17.13倍、23.18倍、10.44倍外,其余caspase的表达量均增加。这些结果与我们之前关于中肠细胞死亡后凝集素分子结合导致消化酶活性降低的研究结果一致。
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引用次数: 1
Four Olethreutinae new to the Italian fauna from Tuscan-Romagnol Appennines (Lepidoptera, Tortricidae) 托斯卡纳-罗马-亚平宁地区意大利区系新发现的四种油蛾科(鳞翅目,油蛾科)
Q3 Agricultural and Biological Sciences Pub Date : 2016-12-19 DOI: 10.4081/JEAR.2016.5856
P. Trematerra, M. Colacci
Lobesia andereggiana (Herrich-Schaffer), Ancylis rhenana (Muller-Rutz), Eucosma agnatana (Christoph) and Lathronympha balearici Diakonoff (Lepidoptera, Olethreutinae) are reported for first time from the Italian fauna. The four species of Lepidoptera Tortricidae were collected from the Tuscan-Romagnol Appennines in the upper Acerreta Valley.
本文首次在意大利区系中报道andereggiana (Herrich-Schaffer)、anylis rhenana (Muller-Rutz)、Eucosma agnatana (Christoph)和Lathronympha balearici Diakonoff(鳞翅目,Olethreutinae)。在阿塞雷塔河谷上游的托斯卡纳-罗马诺-亚平宁山脉采集了4种鳞翅目昆虫。
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引用次数: 0
期刊
Journal of Entomological and Acarological Research
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