Seventy genotypes of winged bean representing five populations, include the, genotypes from India (IN), Thailand (TH), Nigeria (NI), Ghana (GH) and Papua New, Guinea (PNG) were taken for the study. Widely accepted population analysis tools;, POPGene Ver.1.32, GenAlEx Ver. 6.5, Splits Tree Ver.4.15.1, STRUCTURE Ver., 2.3.4 and Structure Harvester Ver.6.0 and MVSP Ver.3.22 were used for data, interpretation. Results revealed that the highest diversity was observed in Thailand, population. Genetic diversity between populations was not related to geography. All the, Indian samples except PT-1 grouped together, one Thailand sample (PT-33) was, totally different from other Thailand samples and PT-69 of PNG highly distinct from, other samples. Cluster analysis revealed that four Thailand samples (PT-31, PT-32, PT-48, and PT-49) two Indian samples (PT-28 and PT-29) and two Thailand samples, (PT-42 and PT-46) are genetically closer with less Euclidean distance of 0, whereas, PT-39 showed a maximum distance of 0.87. From this study, PT-33 (EC 178272), PT-, 69 (EC 38821 P2) and PT-39 (EC 178291) could be labelled as genetic variants.