Plant Gene最新文献_第4页

Induced genetic diversity through EMS mutagenesis in Davana (Artemisia pallens Bess.) and use of ISSR markers to underpin agronomical and chemical traits 通过EMS诱变诱导Davana （Artemisia pallens Bess.）的遗传多样性，并利用ISSR标记支持其农艺和化学性状

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene

Pub Date : 2025-12-01 Epub Date: 2025-08-19 DOI: 10.1016/j.plgene.2025.100543

Arun J. Ganiger , Channayya Hiremath , K. Madhusudan , Manoj kumar Chandrasekaran , V.S. Pragadheesh

Davana (Artemisia pallens Bess.) is an annual aromatic herb that belongs to the Asteraceae family. The current research was attempted to identify elite high essential oil yielding davana mutants with high davanone content. To determine the LD₅₀ value of EMS, seeds of the local davana cultivar “Bangalore” were treated with the chemical mutagen ethyl methane sulphonate (EMS) at varying concentrations of 0.10 %, 0.2 %, 0.3 %, 0.40 %, and 0.50 % for 16 h. The LD₅₀ value was (0.40 % for 16 h) determined by probit analysis. In the M₁ generation, 3000 davana plants were evaluated and forwarded to M₂. Based on visual observation of 1500 M₂ plants, 250 mutants were chosen, and their essential oil and davanone content was assessed. In the M₂ generation, the quantitative traits evaluated and chemical constituents assessed had higher mean and moderate to high phenotypic co-efficient of variation, in comparison to the control. Essential oil yield had a strong and significantly positive correlation to the number of flowers per plant, flower head diameter, and fresh herb yield traits. Based on superiority over control and Mean + 1 SD of M₂ population, 22 and 14 mutants were identified for essential oil yield and davanone content, respectively. Molecular diversity analysis of 16 high essential oil yielding M₂ plants and two check cultivars using ISSR markers revealed an average 79 % polymorphism, with the primer UBC-845 having the highest polymorphic information content. We identified several superior mutants including DM2158 (essential oil content-0.5 %) and DM2103 (Davanone content-70.92 %), indicating that mutation breeding can be an effective strategy to develop novel davana cultivars with higher essential oil and davanone content.

Davana （Artemisia pallens Bess.）是一种一年生芳香草本植物，属于菊科。本研究旨在鉴定具有高davdavone含量的davdavone优质高精油突变体。为了确定EMS的LD50值，用不同浓度的化学诱变剂甲烷磺酸乙酯（EMS）处理本地达瓦纳品种“班加罗尔”的种子，分别为0.10%、0.2%、0.3%、0.40%和0.50%，处理16 h。通过probit分析，LD50值为0.40% （16 h）。在M1代中，对3000株达瓦纳草木进行了评价，并转发到M2上。在1500 M2植株目视观察的基础上，选择250个突变体，对其挥发油和达凡酮含量进行评价。在M2代中，所评价的数量性状和所评价的化学成分与对照相比，具有较高的平均变异和中高的表型变异系数。精油产量与单株花数、花头直径、鲜草产量性状呈极显著正相关。根据M2群体的对照优势和Mean + 1 SD，分别鉴定出22个和14个挥发油产量和davdavone含量的突变体。利用ISSR标记对16株精油高产植物M2和2个对照品种进行分子多样性分析，发现其多态性平均为79%，其中引物UBC-845多态性信息含量最高。本研究鉴定出DM2158（挥发油含量- 0.5%）和DM2103（达瓦酮含量- 70.92%）两个优良突变体，表明突变选育是培育高挥发油和达瓦酮含量达瓦纳新品种的有效策略。

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引用次数: 0

Temporal and spatial expression analysis of AtbZIP9 during seed and silique development in Arabidopsis thaliana (L.) Heynh 拟南芥种子和果实发育过程中AtbZIP9的时空表达分析Heynh

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene

Pub Date : 2025-12-01 Epub Date: 2025-08-12 DOI: 10.1016/j.plgene.2025.100536

Jonatan Illescas-Miranda , Victoria Llanos-Casado , Estefanía Contreras, Néstor Carrillo-Barral, Raquel Iglesias-Fernández

In Arabidopsis thaliana, seed dispersal is mediated by the silique, a specialized fruit that undergoes a complex developmental program involving cell division, expansion, and programmed cell death. Transcription factors (TFs) from the bZIP family are key regulators of these transitions. In this study, we focused on the C-group bZIP TF AtbZIP9 to characterize its expression, potential regulatory roles, and functional relevance during silique development and early seedling growth. Promoter-reporter assays and qPCR analyses revealed that AtbZIP9 is broadly expressed, with strong activity in vascular tissues and the funiculus during early and mid-stages of silique development. AtbZIP9 physically interacts with the S1-group member AtbZIP44, as shown by yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays, supporting the formation of heterodimeric complexes. Despite the lack of major phenotypic alterations in AtbZIP9 knockout mutants during germination and early development—even under salt stress conditions—its co-expression with AtbZIP44 and the CW-modifying gene AtMAN7 suggests a role in transcriptional regulation during silique development. Recent evidence further links AtbZIP9 to ABA-responsive gene expression and identifies it as a likely component of redundant regulatory networks involving other C-group bZIPs. These findings highlight AtbZIP9 as a candidate transcriptional modulator of silique and seed developmental processes, potentially acting in coordination with AtbZIP44 and other factors.

在拟南芥中，种子的传播是由核介导的，核是一种特殊的果实，经历了一个复杂的发育过程，包括细胞分裂、扩增和程序性细胞死亡。来自bZIP家族的转录因子（TFs）是这些转变的关键调节因子。在这项研究中，我们重点研究了c组bZIP TF AtbZIP9，以表征其在硅酸发育和早期幼苗生长中的表达、潜在的调控作用和功能相关性。启动子报告子分析和qPCR分析显示，AtbZIP9广泛表达，在丝质发育早期和中期的维管组织和索细胞中具有较强的活性。酵母双杂交和双分子荧光互补（BiFC）实验表明，AtbZIP9与s1基团成员AtbZIP44相互作用，支持异二聚体复合物的形成。尽管AtbZIP9基因敲除突变体在萌发和早期发育过程中（甚至在盐胁迫条件下）缺乏主要的表型改变，但它与AtbZIP44和cw修饰基因AtMAN7的共表达表明，在硅藻发育过程中，AtbZIP9基因敲除突变体在转录调控中发挥作用。最近的证据进一步将AtbZIP9与aba反应性基因表达联系起来，并确定它可能是涉及其他c组bzip9的冗余调控网络的组成部分。这些研究结果表明，AtbZIP9可能与AtbZIP44和其他因子协同作用，是硅油和种子发育过程的候选转录调节剂。

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引用次数: 0

Cloning and functional characterization of three cinnamate 4-hydroxylase isoforms from Marchantia polymorpha associated with UV-absorbing compounds production 三种肉桂酸4-羟化酶异构体的克隆与功能分析

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene

Pub Date : 2025-12-01 Epub Date: 2025-11-01 DOI: 10.1016/j.plgene.2025.100558

Jiayi Yang, Jian Mo, Baoyun Shan, Xiaochun Qin, Haina Yu

Cinnamate 4-hydroxylase (C4H) catalyzes the second step of the phenylpropanoid pathway to generate precursor for phenolic compounds. As phenylpropanoid-derived specialized metabolites, Ultraviolet (UV)-absorbing compounds (UVACs) exhibit distinct UV-B absorption. In this study, we cloned and functionally characterized three C4H homologs (MpCYP73A1, A2 and A3) from the liverwort Marchantia polymorpha. Bioinformatic analyses confirmed that all MpCYP73 isoforms maintain conserved cytochrome P450 domains characteristic of plant C4H enzymes. Heterologous expression of recombinant MpCYP73 isoforms in yeast microsomes confirmed their functional identity as cinnamate 4-hydroxylases (C4H, EC 1.14.14.91). The enzymes catalyzed trans-cinnamic acid and 3-hydroxycinnamic acid into p-coumaric acid and caffeic acid, respectively. The three MpCYP73 isoforms exhibited distinct kinetic profiles, with MpCYP73A1 displaying the lowest K_m value for trans-cinnamic acid and MpCYP73A3 showing the highest affinity for 3-hydroxycinnamic acid. Furthermore, their catalytic activities toward both substrates also demonstrated marked variation. In addition, MpCYP73A1 displayed the highest transcript abundance, positively correlating with UVACs accumulation. Furthermore, UV-B treatment significantly induced the expression of MpCYP73s and enhanced the total content of methanol-soluble UVACs. These findings provide a basis for further investigation on the critical regulatory role of MpCYP73 isoforms in UVACs biosynthesis in M. polymorpha.

肉桂酸4-羟化酶（C4H）催化苯丙酸途径的第二步生成酚类化合物的前体。作为类苯丙烷衍生的特殊代谢物，紫外线吸收化合物（UVACs）表现出明显的UV- b吸收。本研究从地茅中克隆了3个C4H同源物（MpCYP73A1、A2和A3），并对其进行了功能表征。生物信息学分析证实，所有MpCYP73亚型都保持植物C4H酶特征的保守细胞色素P450结构域。重组MpCYP73亚型在酵母微粒体中的异源表达证实了它们作为肉桂酸4-羟化酶的功能特性（C4H, EC 1.14.14.91）。这些酶分别催化反式肉桂酸和3-羟基肉桂酸生成对香豆酸和咖啡酸。MpCYP73的3个亚型表现出不同的动力学特征，其中MpCYP73A1对反式肉桂酸的Km值最低，MpCYP73A3对3-羟基肉桂酸的Km值最高。此外，它们对两种底物的催化活性也表现出明显的差异。此外，MpCYP73A1的转录物丰度最高，与UVACs积累呈正相关。此外，UV-B处理显著诱导了MpCYP73s的表达，增加了甲醇溶性UVACs的总含量。这些发现为进一步研究MpCYP73亚型在多形霉UVACs生物合成中的关键调控作用提供了基础。

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引用次数: 0

Comprehensive genome-wide study of Glutaredoxin (GRX) gene family in the liverwort Marchantia polymorpha and exploring their roles in abiotic stress tolerance 多形地茅（Marchantia polymorpha） Glutaredoxin （GRX）基因家族的全基因组研究及其在非生物胁迫耐受中的作用

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene

Pub Date : 2025-12-01 Epub Date: 2025-09-11 DOI: 10.1016/j.plgene.2025.100550

Shivani Singh , Garima Saxena , Prachi Mishra , Monica Kumari , Prasanna Dutta , Mehar Hasan Asif , Debasis Chakrabarty

Glutaredoxins (GRXs) are thiol-disulfide oxidoreductases that function as key regulators of redox homeostasis, development, and stress responses in plants. Despite their functional importance, little is known about the GRX gene family in early diverging land plants. In this study, we performed a comprehensive genome-wide identification of GRX genes in the liverwort Marchantia polymorpha and their role in abiotic stresses. A total of 17 MpGRX genes with 2 isoforms (19 MpGRX) containing the conserved Glutaredoxin domain (PF00462) were identified using BLAST, HMMER, and SMART approaches. Gene structure analysis revealed that six MpGRX genes contained a single intron, whereas the remaining genes exhibited more complex structures with three or more introns, suggesting gene expansion and functional diversification. Evolutionary analysis was conducted using non-synonymous (Ka) and synonymous (Ks) substitution rates between MpGRX genes and homologous genes from Physcomitrella patens, Ceratopteris richardii, Pinus taeda, Arabidopsis thaliana, and Oryza sativa. The results indicated generally low Ka values, except in one MpGRX–PtGRX pair with Ka = 1.59, suggesting potential functional divergence in gymnosperms. Ks-based divergence time estimates were consistent with known evolutionary separations. Selection pressure analysis based on Ka/Ks ratios revealed that most GRX gene pairs were under purifying selection, particularly those between M. polymorpha and mosses, ferns, and angiosperms.

This study provides novel insights into the structural diversity, evolutionary history, and selective constraints acting on the GRX gene family in M. polymorpha, offering a foundation for future functional and comparative studies in early land plant lineages.

Glutaredoxins （GRXs）是一种巯基二硫氧化还原酶，在植物氧化还原稳态、发育和胁迫反应中起关键调节作用。尽管GRX基因家族具有重要的功能，但对早期分化的陆生植物的GRX基因家族知之甚少。在这项研究中，我们对地茅（Marchantia polymorpha）的GRX基因进行了全面的全基因组鉴定及其在非生物胁迫中的作用。使用BLAST、HMMER和SMART方法共鉴定了17个MpGRX基因，其中2个同种异构体（19个MpGRX）含有保守的Glutaredoxin结构域（PF00462）。基因结构分析显示，6个MpGRX基因含有1个内含子，其余基因结构更为复杂，含有3个或更多内含子，表明基因扩增和功能多样化。采用非同义替换率（Ka）和同义替换率（Ks）对来自小立小立藓、richardii角羽蛾、taus taeda、拟南芥和Oryza sativa的MpGRX基因与同源基因进行了进化分析。结果表明，除了有一对MpGRX-PtGRX对Ka = 1.59外，其他裸子植物的Ka值普遍较低，提示裸子植物存在潜在的功能分化。基于ks的分化时间估计与已知的进化分离一致。基于Ka/Ks比的选择压力分析表明，大多数GRX基因对处于纯化选择状态，尤其是多形草与苔藓、蕨类和被子植物之间的选择。本研究对多形草GRX基因家族的结构多样性、进化历史和选择限制提供了新的见解，为未来早期陆地植物谱系的功能和比较研究提供了基础。

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引用次数: 0

Gene expression analysis of four Solanum tuberosum L. varieties in vitro induced with Alternaria alternata and Streptomyces werraensis extracts 互交菌和沃拉链霉菌提取物诱导4个龙葵品种的基因表达分析

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene

Pub Date : 2025-12-01 Epub Date: 2025-09-08 DOI: 10.1016/j.plgene.2025.100546

Izdihar Ferhat , Wahiba Harrat , Emre Yörük , Boualem Harfi

Potato (Solanum tuberosum L.) is one of the major tuber food crops worldwide. However, its culture is threatened by several diseases like potato brown leaf spot which is related to Alternaria fungi. This study addresses the challenge of inducing resistance in four potato varieties against brown spot disease by in vitro culture. The approach relies on combining Streptomyces werraensis extracts, reported as potential biocontrol agents, and Alternaria alternata pathogen extracts in the potato culture medium. Initially, potato micropropagation and microtuberization media were optimized, followed by an acclimatization phase and a pathogenicity test on the four varieties (Arizona, Désirée, Spunta and Synergy). Subsequently, potato microtubers were produced in vitro, with microorganisms' extracts included in the culture medium. To assess for induced resistance, qRT-PCR analysis was conducted to evaluate gene expression levels, targeting the most relevant genes (ChtA1, ChtB3, PR1b, PRa1-like, WRKY31 and WRKY75-like). Gene expression analysis revealed significant differences between varieties, with Arizona variety showing the most vigorous response. These distinct varietal responses indicate genotype-specific reactions to the combined treatment of A. alternata and S. werraensis extracts. This research highlights the S. werraensis potential as a biocontrol agent to induce resistance in potatoes to A. alternata. The results support the integration of in vitro culture media supplemented with microorganism extracts for large-scale standardized potato production.

马铃薯（Solanum tuberosum L.）是世界主要的块茎粮食作物之一。但其栽培受到马铃薯褐叶斑病等病害的威胁。本研究解决了4个马铃薯品种在离体培养中诱导抗褐斑病的挑战。该方法依赖于在马铃薯培养基中结合报道为潜在生物防治剂的werraensis链霉菌提取物和交替稻瘟菌病原体提取物。首先，对4个马铃薯品种（Arizona、dsamsisame、Spunta和Synergy）进行了适化和致病性试验。随后，在培养基中加入微生物提取物，在体外生产马铃薯微块茎。为了评估诱导抗性，采用qRT-PCR分析评估基因表达水平，针对最相关的基因（ChtA1、ChtB3、PR1b、pra1 like、WRKY31和wrky75 like）。基因表达分析显示品种间差异显著，以亚利桑那品种表现出最强烈的反应。这些不同的品种反应表明，对互花荆芥和冬荆芥提取物联合处理有基因型特异性反应。本研究强调了稻角霉作为一种生物防治剂诱导马铃薯对稻角霉产生抗性的潜力。研究结果为马铃薯规模化标准化生产中微生物提取物和离体培养基的整合提供了依据。

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引用次数: 0

Integrative leaf transcriptomic and physiological insights into salt stress responses in Eucalyptus: A comparative analysis of two contrasting clones 桉树对盐胁迫反应的综合叶片转录组学和生理学研究：两个对照无性系的比较分析

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene

Pub Date : 2025-12-01 Epub Date: 2025-09-23 DOI: 10.1016/j.plgene.2025.100551

Ravita Tadiya , Santan Barthwal , Harish Singh Ginwal , Hukum Singh , Fateh Singh

Salinity severely limits forest productivity; however, the molecular basis of tolerance in Eucalyptus remains unclear. We investigated the physiological and transcriptomic responses of a salt-tolerant clone (B-112) and a salt-sensitive clone (W-12) subjected to 80 days of severe salinity (EC ≥ 20 dS/m). Both clones showed reductions in photosynthesis, stomatal conductance, internal CO₂ concentration, and transpiration; however, the extent of decline was considerably smaller in B-112, indicating superior physiological resilience. RNA-seq profiling revealed the targeted reprogramming of 1025 genes in B-112, compared with a broad, less coordinated response involving 14,994 genes in W-12. In B-112, enrichment analyses highlighted the strong activation of abscisic acid (ABA) and ethylene signaling, mitogen-activated protein kinases (MAPKs) cascades, carbohydrate metabolism, antioxidant defense, and protein folding pathways. Key salt-responsive genes included Dehydrins (DHN1), Ethylene Response Factors (ERFs), cupredoxins, and expansins, indicating coordinated osmotic adjustment, cell-wall modification, and stress signaling. Conversely, W-12 displayed extensive upregulation of ubiquitination and vesicle trafficking genes, alongside repression of photosynthesis and chloroplast-related processes, consistent with a damage-induced rather than adaptive program. qRTPCR validation confirmed high concordance with RNAseq results. Together, these findings reveal that B-112 sustains salt tolerance through proactive and focused transcriptomic reprogramming, while W-12 exhibits reactive stress-triggered responses, providing candidate targets for breeding and genetic improvement of Eucalyptus in saline environments.

盐度严重限制了森林生产力；然而，桉树耐受性的分子基础尚不清楚。研究了耐盐无性系（B-112）和盐敏感无性系（W-12）在80天高盐度（EC≥20 dS/m）条件下的生理和转录组反应。两个无性系的光合作用、气孔导度、内部CO 2浓度和蒸腾作用均降低；然而，B-112的下降程度要小得多，这表明B-112具有更强的生理弹性。RNA-seq分析揭示了B-112中1025个基因的靶向重编程，而W-12中涉及14994个基因的广泛、不太协调的反应。在B-112中，富集分析强调了脱落酸（ABA）和乙烯信号、丝裂原活化蛋白激酶（MAPKs）级联、碳水化合物代谢、抗氧化防御和蛋白质折叠途径的强激活。关键的盐响应基因包括脱氢蛋白（DHN1）、乙烯响应因子（ERFs）、铜氧还毒素（cupredoxins）和扩张蛋白（expansins），它们指示协调的渗透调节、细胞壁修饰和胁迫信号。相反，W-12表现出泛素化和囊泡运输基因的广泛上调，同时抑制光合作用和叶绿体相关过程，这与损伤诱导而非适应性程序相一致。qRTPCR验证证实与RNAseq结果高度一致。总之，这些发现表明B-112通过主动和集中的转录组重编程维持耐盐性，而W-12表现出应激触发的反应性，为桉树在盐环境下的育种和遗传改良提供了候选靶点。

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引用次数: 0

Identification and characterization of the CONSTANS-like gene family and its expression profiling under salt treatment in alfalfa (Medicago sativa L.) 紫花苜蓿（Medicago sativa L.） CONSTANS-like基因家族的鉴定、鉴定及其盐处理下的表达谱分析

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene

Pub Date : 2025-12-01 Epub Date: 2025-08-29 DOI: 10.1016/j.plgene.2025.100544

Shuzhi Ma, Yiping Wei, Qinyan Bao, Zhaozhu Wen, Mengli Luo, Wenxuan Du

Alfalfa (Medicago sativa L.) is a globally cultivated, high-quality forage crop. The CONSTANS-LIKE (COL) genes play crucial roles in regulating flowering time and stress responses in plants. However, the functional characterization of COL genes in alfalfa remain largely unexplored. In this study, we conducted a genome-wide analysis of the Xinjiang Daye alfalfa genome and identified 36 MsCOL genes. Multiple sequence alignment confirmed that all MsCOL genes contain conserved B-box and CCT (CO, CO-like and TOC1) domains. Phylogenetic analysis and gene structure analyses classified the MsCOL genes into two subclades, exhibiting similar exon-intron organization and conserved motif distributions. Chromosomal mapping revealed that these genes are distributed across 22 chromosomes. Expression profiling under salt stress demonstrated that MsCOL genes exhibit expression patterns, indicating their potential involvement in abiotic stress responses. These findings enhance our understanding of MsCOL gene function in alfalfa and provide a theoretical basis for breeding salt-tolerant alfalfa cultivars.

苜蓿（Medicago sativa L.）是一种全球种植的优质饲料作物。CONSTANS-LIKE （COL）基因在调控植物开花时间和胁迫反应中起着至关重要的作用。然而，在苜蓿中COL基因的功能表征仍未得到充分的研究。在本研究中，我们对新疆大冶苜蓿基因组进行了全基因组分析，鉴定出36个MsCOL基因。多个序列比对证实，所有MsCOL基因都含有保守的B-box和CCT （CO、CO样和TOC1）结构域。系统发育分析和基因结构分析将MsCOL基因分为两个亚支系，具有相似的外显子-内含子组织和保守的基序分布。染色体图谱显示这些基因分布在22条染色体上。盐胁迫下的表达谱表明，MsCOL基因表现出表达模式，表明它们可能参与非生物胁迫反应。这些发现增加了我们对紫花苜蓿MsCOL基因功能的认识，为选育耐盐紫花苜蓿品种提供了理论依据。

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引用次数: 0

Genome-wide identification of the Delay of Germination (DoG1) gene and ethrel mediated regulation of seed dormancy in peanut 花生发芽延迟基因DoG1的全基因组鉴定及乙烯基介导的种子休眠调控

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene

Pub Date : 2025-12-01 Epub Date: 2025-10-24 DOI: 10.1016/j.plgene.2025.100555

Chandramohan Sangh, Swati Kasundra, Praveen Kona, Radhakrishnan Thankappan, Sandip Kumar Bera

The DoG1 (Delay of Germination 1) gene family has emerged as an important regulator in the post-genomic era owing to its critical roles in seed dormancy, plant growth, and development. Although extensively studied in several crops, its characterization in peanut (Arachis hypogaea) has not yet been undertaken. To address this gap, the present study aimed to identify DoG1-like (AhDoG1L) genes in peanut and investigate structural features, expression, and evolutionary relationships. Using a peanut-specific HMM, we identified 34 AhDoG1L genes, all of which contained a conserved DoG1 domain, with some also harboring a bZIP domain. Protein sequence analysis predicted nuclear localization, and phylogenetic classification grouped these genes into three subclades, with tandem duplications preferentially distributed at chromosome termini. Comparative synteny and orthology analyses with soybean and sunflower revealed conserved evolutionary relationships. Promoter analysis identified 10 conserved motifs and multiple cis-acting regulatory elements responsive to light, hormones, and low temperature, suggesting diverse regulatory mechanisms. Functional annotation through GO enrichment and protein–protein interaction networks further supported roles in transcriptional regulation and dormancy control. Expression analysis using qRT-PCR demonstrated that 11 AhDoG1L genes (p < 0.05) showed significantly higher expression in freshly harvested seeds than in germinated seeds, indicating a role in seed dormancy in the TG37 genotype. Moreover, ethrel treatment in PBS 15014 led to the identification of AhDoG1-L34, AhDoG1-L30, and AhDoG1-L22 (p < 0.05), highlighting responsiveness to ethrel. Collectively, these findings provide the first comprehensive overview of the AhDoG1 gene family in peanut, offering insights into their potential involvement in regulating seed dormancy and germination.

由于在种子休眠、植物生长和发育中起着关键作用，DoG1（延迟发芽1）基因家族在后基因组时代成为一个重要的调控基因。虽然在几种作物中进行了广泛的研究，但其在花生（Arachis hypogaea）中的特性尚未进行。为了解决这一空白，本研究旨在鉴定花生中dog1样（AhDoG1L）基因，并研究其结构特征、表达和进化关系。使用花生特异性HMM，我们鉴定了34个AhDoG1L基因，所有这些基因都包含一个保守的DoG1结构域，其中一些还包含一个bZIP结构域。蛋白质序列分析预测了核定位，系统发育分类将这些基因分为三个亚支，串联重复优先分布在染色体末端。与大豆和向日葵的比较合成和同源分析显示了保守的进化关系。启动子分析发现了10个保守的基序和多个对光、激素和低温敏感的顺式调控元件，表明调控机制的多样性。通过氧化石墨烯富集和蛋白-蛋白相互作用网络的功能注释进一步支持了其在转录调控和休眠控制中的作用。qRT-PCR表达分析显示，11个AhDoG1L基因在新鲜收获种子中的表达量（p < 0.05）显著高于发芽种子，表明TG37基因型在种子休眠中起作用。此外，在PBS 15014中处理乙烯利导致AhDoG1-L34、AhDoG1-L30和AhDoG1-L22的鉴定（p < 0.05），突出了对乙烯利的反应性。总的来说，这些发现提供了花生中AhDoG1基因家族的第一个全面概述，为它们在调节种子休眠和萌发中的潜在参与提供了见解。

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引用次数: 0

Induced mutagenesis confers earliness and salt tolerance in ricebean (Vigna umbellata) 诱导诱变提高了黄豆早熟性和耐盐性。

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene

Pub Date : 2025-12-01 Epub Date: 2025-10-26 DOI: 10.1016/j.plgene.2025.100556

Soumik Dey , Aditya Pratap Singh , Sutanu Sarkar , Kalyan Jana , Mojtaba Kordrostami , Mehdi Rahimi

Ricebean (Vigna umbellata [Thunb.] Ohwi and Ohashi) is an underutilized legume valued for its dual role as a protein-rich pulse and high-quality forage crop. However, its commercial adoption is limited due to late maturity and susceptibility to salinity stress. Eleven M5 mutant lines derived from gamma-irradiated seeds of the late-maturing variety BRB-1 were evaluated under single-site field trials and controlled salinity assays. Several mutants flowered and matured ∼2 weeks earlier than BRB-1 and showed higher mean forage protein and seed/forage yield under these single-site trials. One line displayed superior germination and seedling performance under in-vitro salinity screening. These results indicate that induced mutagenesis can generate promising early-maturing and salt-tolerant candidates; however, multi-location and multi-season evaluations and physiological/molecular validation are required before recommending release or broad dual-purpose deployment.

黄豆（黄豆）[拇指]。[Ohwi和Ohashi]是一种未被充分利用的豆科植物，因为它具有富含蛋白质的豆类和高质量的饲料作物的双重作用。然而，由于成熟度较晚且易受盐度胁迫，其商业应用受到限制。以晚熟品种BRB-1的γ辐照种子衍生的11个M5突变系进行了单点田间试验和控制盐度分析。在这些单点试验中，一些突变体比BRB-1早开花和成熟约2周，并显示出更高的平均饲料蛋白和种子/饲料产量。其中一个品系在体外盐度筛选下表现出优异的萌发和出苗性能。这些结果表明，诱变可以产生有希望的早熟耐盐候选品种；然而，在建议释放或广泛的双重用途部署之前，需要进行多地点和多季节的评估和生理/分子验证。

引用次数: 0

Exploring the genetic diversity of common bean germplasm: insights into Andean gene pool variability 探索普通豆类种质资源的遗传多样性：安第斯基因库变异的见解

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene

Pub Date : 2025-12-01 Epub Date: 2025-08-18 DOI: 10.1016/j.plgene.2025.100540

Cecilia Luvizutti Ferreira Silva , Dario Grattapaglia , Paula Arielle Mendes Ribeiro Valdisser , Paula Pereira Torga , Alessandra da Cunha Moraes Rangel , Claudio Brondani , Alexandre Siqueira Guedes Coelho , Tereza Cristina de Oliveira Borba , Rosana Pereira Vianello

Common bean (Phaseolus vulgaris) is among the most widely consumed legumes globally, with Brazil playing a crucial role in preserving and expanding its existing genetic diversity. This study aimed to characterize a subset of 863 common bean accessions from Brazil's Gene Banks, mainly of Andean origin. A germplasm collection was genotyped with 4275 SNPs using the EMBRAPA Multispecies 65KChip. Population structure analysis revealed two main groups: Andean (n = 558) and Middle American (n = 267), along with 38 admixed accessions. Andean group exhibited lower gene diversity (GD = 0.086), and allelic richness (A_R = 1.83) compared to the Middle American group (GD = 0.270, A_R = 1.98). Brazilian landraces in the Andean and Middle American groups showed potentially lower overall diversity, emphasizing the need for conservation efforts to preserve these genetic resources. The Andean Brazilian core collection (n = 221; GD = 0.095) encompasses the active collection diversity evaluated (n = 337; GD = 0.093). A total of 26 SNPs potentially under selection, mainly associated with plant development and defense, were identified. Georeferencing landraces using climate maps identified potentially valuable varieties adapted to drought (e.g., BGF0011779, BGF0012528, BGF0013826) and high temperatures and low-fertility soils (e.g., BGF0016128, BGF0013871), highlighting their relevance for conservation and sustainable use in breeding programs. Accessions' photographs showcased a wide range of morphological diversity, colors, types, shapes, and sizes of beans. Our findings reveal a significant genetic diversity among common bean germplasm, offering practical breeding opportunities and enhancing the value of gene bank collections. Certain landraces show potential for adapting to challenging climatic conditions, making them promising subjects for further adaptation studies.

普通豆（Phaseolus vulgaris）是全球消费最广泛的豆类之一，巴西在保护和扩大其现有遗传多样性方面发挥着至关重要的作用。本研究旨在对来自巴西基因库的863个普通豆品种进行特征分析，这些品种主要来自安第斯山脉。利用EMBRAPA multi - species 65KChip对一份种质进行4275个snp基因分型。种群结构分析显示安第斯（n = 558）和中美洲（n = 267）两个主要种群，以及38个混合种群。安第斯组基因多样性（GD = 0.086）和等位基因丰富度（AR = 1.83）低于中美洲组（GD = 0.270, AR = 1.98）。安第斯山脉和中美洲地区的巴西本土人种显示出潜在的整体多样性较低，这强调了保护这些遗传资源的必要性。安第斯山脉巴西岩心标本（n = 221; GD = 0.095）包含评估的活跃标本多样性（n = 337; GD = 0.093）。共鉴定出26个可能处于选择中的snp，主要与植物发育和防御有关。利用气候图对地方品种进行地理参考，确定了适应干旱（如BGF0011779、BGF0012528、BGF0013826）和高温低肥力土壤（如BGF0016128、BGF0013871）的潜在有价值品种，强调了它们在育种计划中的保护和可持续利用的相关性。这些照片展示了豆类的形态多样性、颜色、类型、形状和大小。我们的研究结果揭示了普通豆类种质资源具有显著的遗传多样性，为实际育种提供了机会，并提高了基因库收藏的价值。某些地方品种显示出适应具有挑战性的气候条件的潜力，使它们成为进一步适应研究的有希望的主题。

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