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Trichoderma fungi as an agricultural biological control in Mexico 木霉在墨西哥的农业生物防治作用
Q3 Agricultural and Biological Sciences Pub Date : 2023-08-01 DOI: 10.5154/r.rchsh.2022.11.015
María Antonia Flores-Córdova, Miguel Matas, Sandra Pérez, María Rodríguez, Nora Salas, Mayra Soto, Esteban Sánchez-Chávez
Environmental pollution, food sufficiency, and health are among the main challenges for sustainable crop production in Mexico. The main tool for plant protection is chemical pesticides, but environmental safety and risks to animal and human health have prompted the community and scientists to propose alternatives for their integrated management. This has encouraged the inclusion of biological products, such as species of the antagonistic fungus Trichoderma spp., which act against pests and pathogens in different crops, and are environmentally benign, effective and economically viable. The aim of this paper was to compile information on Trichoderma spp. regarding their taxonomy, ecology, use as biological control, isolation, culture, morphological and molecular identification, mode of action, distribution of native species, and availability of international commercial products and those produced in Mexico. For this purpose, information was selected from indexed journals and suppliers of Trichoderma spp. on open data digital platforms. The exposed information could be useful for Mexican farmers, to know, accept and use biological control with Trichodermaspp. in their crops, for the benefit of quality agriculture and pesticide-free crops. In addition, research could be generated to develop new autochthonous products based on Trichodermaspp. that are more effective against pests and diseases native to Mexico.
环境污染、粮食充足和健康是墨西哥可持续作物生产面临的主要挑战。植物保护的主要工具是化学农药,但环境安全和对动物和人类健康的风险促使社区和科学家提出了对其综合管理的替代方案。这鼓励了生物制品的纳入,例如拮抗真菌木霉,它对不同作物的害虫和病原体起作用,对环境无害,有效,经济上可行。本文旨在收集有关木霉的分类、生态学、生物防治、分离、培养、形态和分子鉴定、作用方式、本地种分布、国际商业产品和墨西哥生产产品的可用性等方面的资料。为此,从开放数据数字平台上的索引期刊和木霉供应商中选择信息。暴露的信息可能有助于墨西哥农民了解、接受和使用木霉的生物防治。在他们的作物中,有利于优质农业和无农药作物。此外,还可以开展以木霉为原料开发新的本土产品的研究。对墨西哥本土的病虫害更有效。
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引用次数: 0
Solanum demissum Lindl. en el mejoramiento genético de la papa Solanum demissum Lindl。在马铃薯的遗传改良中
Q3 Agricultural and Biological Sciences Pub Date : 2023-08-01 DOI: 10.5154/r.rchsh.2023.01.001
Gabriela Díaz-García, Guillermo Andrés Enciso-Maldonado, Héctor Lozoya-Saldaña
S olanum demissum Lindl. is one of the most valued and used species for potato (Solanum tuberosum L.) breeding due to its richness in genes for resistance to various biotic and abiotic factors. Over the years, it has been a plant of interest, mainly for its resistance to potato late blight (caused by Phytophthora infestans [Mont.] de Bary.), which has led to crosses between S. demissum and S. tuberosum to obtain resistant cultivars. It has also been used for identifying physiological races of P. infestans and, recently, it has been associated with glycoalkaloids and antioxidant compounds, whose potential uses are of interest to various industries. Since there is great potential in the species S. demissum, not only as a source of resistance genes but also as a source of important bioactive compounds, this review summarizes the research conducted on the use of Solanum demissum in the breeding of cultivated potato, as well as its potential uses in other areas. In addition, areas of opportunity and limitations for breeding for resistance to late blight are highlighted to serve as a starting point for the development of new research or lines.
山楂属植物。是马铃薯(Solanum tuberosum L.)育种中最有价值和利用的品种之一,因为它具有丰富的抗各种生物和非生物因素的基因。多年来,它一直是一种令人感兴趣的植物,主要是因为它能抵抗马铃薯晚疫病(由疫霉引起的)。] de Bary.),这导致了葡萄球菌与葡萄球菌之间的杂交,以获得抗性品种。它还被用于鉴定病原菌的生理小种,最近,它已与糖生物碱和抗氧化化合物联系起来,其潜在用途引起了各种工业的兴趣。由于半芒葵不仅是抗性基因的来源,而且是重要的生物活性化合物的来源,因此本文综述了半芒葵在马铃薯栽培育种中的应用研究及其在其他领域的潜在应用。此外,还强调了育种抗晚疫病的机会和限制领域,以作为开发新研究或新品系的起点。
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引用次数: 0
Automatic identification of avocado fruit diseases based on machine learning and chromatic descriptors 基于机器学习和颜色描述符的鳄梨果实病害自动识别
Q3 Agricultural and Biological Sciences Pub Date : 2023-08-01 DOI: 10.5154/r.rchsh.2023.04.002
Ulises Enrique Campos-Ferreira, Juan Manuel González-Camacho, José Alfredo Carrillo-Salazar
Timely identification of phytosanitary problems in agricultural crops is essential to reduce production losses. Artificial intelligence algorithms facilitate their rapid and reliable identification. In this research, three learning classifiers, namely random forest (RF), support vector machine (SVM) and multilayer perceptron (MLP), were evaluated to identify three target classes (healthy fruit, anthracnose [Colletotrichum spp.] and scab [Sphaceloma perseae]) from digital fruit images. Two color descriptor extraction techniques (region selection and image subsampling) were compared with the RF classifier, and an overall classification accuracy (ACC) of 98±0.03 % with region selection and 84±0.08 % with subsampling was obtained. Subsequently, the classifiers were evaluated with color descriptors extracted with region selection. RF and MLP were superior to SVM, with an ACC of 98±0.03 %. Scab and anthracnose were identified with an F1 score of 98 %. %. The high performance of the classifiers shows the potential for applying artificial intelligence paradigms to identify phytosanitary problems in agricultural crops.
及时发现农作物的植物检疫问题对于减少生产损失至关重要。人工智能算法促进了它们快速可靠的识别。本研究采用随机森林(random forest, RF)、支持向量机(support vector machine, SVM)和多层感知器(multilayer perceptron, MLP)三种学习分类器,从数字水果图像中识别健康水果、炭疽病(Colletotrichum spp)和痂病(Sphaceloma perseae)三种目标类。将两种颜色描述符提取技术(区域选择和图像子采样)与RF分类器进行比较,区域选择和子采样的总体分类准确率分别为98±0.03%和84±0.08%。随后,使用区域选择提取的颜色描述符对分类器进行评估。RF和MLP优于SVM, ACC为98±0.03%。结痂和炭疽病的F1分为98%。%。分类器的高性能显示了应用人工智能范式识别农作物植物检疫问题的潜力。
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引用次数: 0
Sugar transporters in plant-beneficial endophytic fungus and plant-phytopathogenic nematode interactions 植物有益内生真菌和植物致病线虫相互作用中的糖转运体
Q3 Agricultural and Biological Sciences Pub Date : 2023-08-01 DOI: 10.5154/r.rchsh.2022.11.016
Luis Yobani Gayosso-Rosales, Reyna Isabel Rojas-Martínez, Stefan de Folter, Daniel Leobardo Ochoa-Martínez, Manuel Silva-Valenzuela, Emma Zavaleta-Mejía
The transport of sugars, a product of photosynthesis, involves the distribution of carbohydrates from synthesis organs, such as leaves, to the tissues of demand organs such as roots, stems, flowers, fruits and seeds. Their mobilization occurs through the plasmodesmata or apoplast by means of membrane transporters, which are proteins encoded by the so-called sugar transporter genes. Their expression in plants is modified by the presence of microorganisms that colonize them. Studies on their expression in plantnematode interactions are relatively recent, and in plant-beneficial endophyte fungus interactions they have been carried out mainly with arbuscular mycorrhizal fungi. In this review, information on the current state of knowledge of sugar transporters in plantendophyte fungus and plant-nematode interactions is summarized.
糖是光合作用的产物,它的运输涉及碳水化合物从合成器官(如叶子)到需求器官(如根、茎、花、果实和种子)的组织的分布。它们的动员通过胞间连丝或外质体通过膜转运蛋白发生,膜转运蛋白是由所谓的糖转运基因编码的蛋白质。它们在植物中的表达被定殖于它们的微生物的存在所改变。它们在植物线虫相互作用中的表达研究相对较晚,而在植物有益内生真菌相互作用中,它们主要是在丛枝菌根真菌中进行的。本文综述了植物真菌和植物-线虫相互作用中糖转运体的研究现状。
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引用次数: 0
First report of Fusarium oxysporum f. sp. niveum race 1 as causal agent of vascular wilt of watermelon in Mexico 墨西哥西瓜枯萎病病原菌尖孢镰刀菌1号初报
Q3 Agricultural and Biological Sciences Pub Date : 2023-08-01 DOI: 10.5154/r.rchsh.2022.11.014
Irasema Vargas-Arispuro, Irene Iliana Ramírez-Bustos, Alda Alejandra Arratia-Castro, Daniel Bárcena-Santana, Ernesto Fernández-Herrera
Vascular wilt (Fusarium oxysporum f. sp. niveum [Fon]) is the main fungal disease of watermelon crops worldwide and can be caused by any of the four known races of this fungus (race 0, 1, 2 and 3). In Mexico, there are no reports of the presence of any of these races. The presence of plants with wilt and vascular necrosis has been observed in commercial watermelon plantations in the municipality of Hermosillo, Sonora, Mexico, mainly during the spring-summer growing seasons. The aim of this study was to identify the Fon race causing vascular wilt in watermelon plants in Hermosillo, Sonora. Three fungal isolates were collected from symptomatic plants. Based on their morphological characteristics, DNA amplification of the isolates (with specific primers Fon-1/Fon-2, FONSIX6F/FONSIX6R and FNR3-F/FNR3-R, which allow differentiating Fon race 1, 2 and 3) and inoculation into three differential watermelon cultivars, the three isolates (UESFON01, UESFON02 and UESFON03) were identified as F. o. f. sp. niveum race 1. To our knowledge, this is the first report where F. o. f. sp. niveum race 1 is identified as the causal agent of wilt, vascular necrosis and plant death in watermelon crops in Mexico.
维管枯萎病(Fusarium oxysporum f. sp. niveum [Fon])是世界范围内西瓜作物的主要真菌疾病,可以由这种真菌的四种已知菌种(0、1、2和3种)中的任何一种引起。在墨西哥,没有任何这些菌种存在的报道。在墨西哥索诺拉州埃莫西约市的商业西瓜种植园中发现了枯萎和维管坏死的植物,主要发生在春夏生长季节。本研究的目的是鉴定引起索诺拉埃莫西约西瓜维管枯萎病的Fon小种。从有症状植物中分离到3株真菌。根据形态特征,对分离菌株进行DNA扩增(用特异性引物Fon-1/Fon-2、FONSIX6F/FONSIX6R和FNR3-F/FNR3-R,可分化Fon小种1、2和3),并接种到3个西瓜差异栽培品种中,鉴定为foff.sp . niveum小种1。据我们所知,这是首次报道f.o.f. sp. niveum 1号种被确定为墨西哥西瓜作物枯萎、维管坏死和植株死亡的致病因子。
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引用次数: 0
Mycorrhizal fungi and phosphate fertilization in the formation of seedlings of vine rootstock ‘IAC-766’ Campinas 菌根真菌和磷肥在藤本砧木‘IAC-766’Campinas幼苗形成中的作用
Q3 Agricultural and Biological Sciences Pub Date : 2023-04-28 DOI: 10.5154/r.rchsh.2022.05.008
Carlos Vilcatoma-Medina, Marcos A. Dolinski, J. Mendoza-Cortez, Amaro A. Campos-de Azeredo, L. A. Biasi, Mauro B. Dias Tofanelli
Arbuscular mycorrhizal fungi (AMF) are beneficial microorganisms that form symbioses with vines, showing improvements favorably in the growth and nutrition of seedlings after rooting them. This work aimed to evaluate the effects of AMF species inoculation and phosphate fertilization on rooted cuttings of vine rootstocks in a greenhouse. Woody rootstock cuttings from 'IAC 766' Campinas vines were prepared to promote rooting. After 45 days, the cuttings were transplanted and subjected to treatments that consisted of the inoculation of different species of AMF and fertilization with two doses of phosphorus (simple superphosphate) plus the control. The treatments were randomly assigned following a completely randomized design with eight replications for each treatment. After 90 days, growth parameters, macro and micronutrient content (shoot and root), root colonization and mycorrhizal effect of the extracted nutrients were evaluated. The inoculation with AMF species influenced the growth parameters, root colonization and extraction of macro and micronutrients from the cuttings of the 'IAC 766' rootstock, highlighting the inoculation with species of the genus Acaulospora, either individually or associated. Phosphorous fertilization affected growth parameters and extraction of macro and micronutrients, but these were to a lesser degree compared to that verified by the inoculation of AMF species.
丛枝菌根真菌(AMF)是与藤蔓形成共生体的有益微生物,在生根后对幼苗的生长和营养表现出有利的改善。本工作旨在评估AMF物种接种和磷酸盐施肥对温室葡萄砧木生根插条的影响。从‘IAC766’Campinas葡萄藤上制备木质砧木插条以促进生根。45天后,将插条移植并进行处理,包括接种不同种类的AMF和用两剂磷(过磷酸钙)加对照施肥。按照完全随机设计随机分配治疗,每个治疗有八个重复。90天后,对提取的营养物质的生长参数、宏观和微量营养素含量(地上部和根部)、根系定殖和菌根效应进行了评估。AMF物种的接种影响了生长参数、根系定殖以及从“IAC 766”砧木的插条中提取大量和微量营养素,突出了单独或相关的Acaulospora属物种的接种。磷肥影响生长参数以及宏营养素和微量营养素的提取,但与接种AMF物种相比,这些影响程度较小。
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引用次数: 0
Reduction of seed number in ‘‘Yashar’’ mandarin by application of copper sulfate and different pollen grains sources 施用硫酸铜和不同花粉粒源对“雅夏”柑种子数量的影响
Q3 Agricultural and Biological Sciences Pub Date : 2023-04-28 DOI: 10.5154/r.rchsh.2022.05.007
D. Hashemabadi, B. Kaviani, H. Gholipour, M. Ghasemi, Mojtaba Khorrami Raad
The citrus trade market welcomes cultivars with different characteristics such as being totally seedless or having fewer seeds, having thin and vividness skin, high ratio of sugar to acid, being watery, easy peeling and relatively large fruit. Furthermore, a wide range of cultivars should be available according to fruit ripening time from very early (precocious) to very late (serotinous). ‘Yashar’ mandarin is a hybrid obtained by crossing of the ‘Minneola’ tangelo and ‘Changsha’ mandarin. The fruits of this cultivar grow in the northern regions of Iran, and it ripen in May, thus, they are considered the late-ripening mandarin cultivars. One of the deficiencies of this cultivar is that it is full of seeds (25-30). Two separate experiments were performed to reduce the number of seeds in ‘Yashar’ mandarin. In the first experiment; the effect of pollen grains of different citrus cultivars (‘Valencia’ orange, ‘Hamlin’ and sour orange) and a free-pollination treatment as a control group was evaluated. In the second experiment, the effect of foliar application of copper sulfate at the rate of 25 mg·L-1 was conducted in stages 30, 60 and 80 % of open flowers. One free pollination treatment as a control group was evaluated as well. The results showed that the highest index of fruit technology (multiplication of the percentage of the extract in the soluble solids divided by 100) was obtained by pollination with ‘Hamlin’ and sour orange pollen grains. The foliar spraying with copper sulfate, when 30 % of the flowers had opened, among all the treatments evaluated in the study, reduced the number of seeds in the Yashar mandarin cultivar.
柑橘贸易市场欢迎完全无籽或少籽、果皮薄而鲜嫩、糖酸比高、水分多、易脱皮、果实较大等不同特点的品种。此外,根据果实成熟时间,从很早(早熟)到很晚(晚熟),应该有广泛的品种可供选择。“雅沙尔”柑橘是由“明尼奥拉”柑橘和“长沙”柑橘杂交而成的。这种品种的果实生长在伊朗北部地区,它在5月成熟,因此,它们被认为是晚熟的柑橘品种。该品种的不足之一是种子丰富(25-30)。为了减少“雅沙尔”柑橘的种子数量,进行了两次单独的实验。在第一个实验中;评价了不同柑橘品种(‘瓦伦西亚’橙、‘哈姆林’橙和酸橙)花粉粒和自由授粉处理作为对照组的效果。第二个试验,在开花30期、60期和80期分别施用硫酸铜25 mg·L-1。一个自由授粉处理作为对照组也进行了评估。结果表明,用‘Hamlin’和酸橙花粉粒授粉获得的果实技术指标最高(提取液占可溶性固性物的百分比乘以100)。在本研究评估的所有处理中,当花开放30%时,叶面喷施硫酸铜可减少雅夏柑品种的种子数量。
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引用次数: 0
Effect of two flooding levels on growth and physiological parameters in peach rootstock ‘Nemared’ 两个淹水水平对桃砧木‘Nemared’生长和生理参数的影响
Q3 Agricultural and Biological Sciences Pub Date : 2023-04-28 DOI: 10.5154/r.rchsh.2022.09.012
V. H. Ziegler, A. Weibel, P. Insausti
While peach tree is very sensitive to waterlogging, there are no works on the effect of the flooding over only part of its root system. The objective of this work was to investigate physiological and growth responses of Nemared rootstock to partial flooding of roots. Treatments were performed for 6-days: control trees, half-flooded, and fully-flooded. Both flooding treatments negatively affected the vegetative growth, stomatal conductance, chlorophyll content and leaf water potential. The response observed in the half-flooded treatment was intermediate between the control and fully-flooded treatment. The fastest response of stomatal conductance to soil flooding, before differences among treatments in leaf water potential were perceived, suggested that there should be a stomatal closure mechanism that responds to a signal that arises from flooded roots. The importance of this work, lies in showing the negative effects of unnoticed and partial floods, frequently occurring in peach trees.
虽然桃树对内涝非常敏感,但目前还没有研究洪水对其部分根系的影响。本研究的目的是研究Nemared砧木对根系部分淹水的生理和生长反应。处理时间为6天:对照树、半淹没和完全淹没。两种淹水处理均对营养生长、气孔导度、叶绿素含量和叶片水势产生负面影响。在半淹没处理中观察到的反应介于对照和全淹没处理之间。气孔导度对土壤淹水的最快反应,在感知到不同处理之间叶片水势的差异之前,表明应该有一种气孔关闭机制来响应淹水根系产生的信号。这项工作的重要性在于展示了桃树上经常发生的未被注意的局部洪水的负面影响。
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引用次数: 0
Microperforated-based modified atmosphere incorporated with vapors of oregano essential oil to preserve blackberry fruits in postharvest 以微孔为基础的改性大气与牛至精油的蒸汽相结合,以保存黑莓果实在采收后
Q3 Agricultural and Biological Sciences Pub Date : 2022-12-01 DOI: 10.5154/r.rchsh.2022.07.010
Angélica Martínez-López, S. Valle-Guadarrama, D. Guerra-Ramírez, M. L. Arévalo-Galarza, R. Sánchez-Fernández
Blackberry fruits have a shelf life of 3 d at ambient temperature, due to fungal development. Essential oils (Eo) can increase shelf life because of their antifungal potential. The objective was to evaluate the use of modified atmosphere packaging incorporated with vapors of an Eo to prolong shelf life of blackberry fruits. The methodology included the use of oregano Eo to inhibit fungi development. Eo was analyzed with gas chromatography-mass spectrometry. The minimum inhibitory concentration of Eo to inhibit fungi in blackberry fruits was determined in vitro and in vivo. The storage of blackberry fruits under modified atmosphere with vapors of oregano Eo was evaluated at ambient temperature. The compounds linalool, o-cymene, and thymol were identified as the main constituents of Eo. Based on fruits with fungal development, isolates were made in culture media and the presence of Aspergillus carbonarius, Alternaria alternata, and Penicillum digitatum was identified. A minimum inhibitory concentration of Eo in the head space of fruits was found with value of 0.8 μL·mL-1. The handling of blackberry fruits in modified atmosphere with micro-perforation and exposition to volatilized Eo (MAP-Eo) reduced the rate of weight loss, the loss of consistency, and that of alteration of color attributes, which allowed maintaining a better appearance during 6-7 d at 23 °C. MAP-Eo kept the content of phenolic compounds and anthocyanins, as well as the antioxidant capacity, without significant changes. In conclusion, MAP-Eo is a useful alternative to extend shelf life of blackberry fruits.
由于真菌的发展,黑莓果实在室温下的保质期为3天。精油(Eo)可以延长保质期,因为它们具有抗真菌的潜力。目的是评估使用改性大气包装与蒸汽的Eo延长黑莓果实的保质期。该方法包括使用牛至Eo来抑制真菌的生长。采用气相色谱-质谱联用分析Eo。在体外和体内测定了Eo对黑莓果实中真菌的最低抑制浓度。在常温条件下,研究了牛至叶精油修饰气氛对黑莓果实的贮藏效果。经鉴定其主要成分为芳樟醇、邻花香烃和百里香酚。根据真菌发育的果实,在培养基中分离出碳曲霉、互交霉和指状青霉。Eo在果实顶空的最低抑菌浓度为0.8 μL·mL-1。在带有微穿孔和挥发性Eo (MAP-Eo)的改性气氛中处理黑莓果实,降低了重量损失率、稠度损失率和颜色属性的改变率,从而使黑莓果实在23°C下保持6-7 d的较好外观。MAP-Eo的酚类化合物和花青素含量及抗氧化能力没有明显变化。综上所述,MAP-Eo是延长黑莓果实保质期的有效替代品。
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引用次数: 0
Growth of ‘Sweet Nufar’ basil (Ocimum basilicum L.) inoculated with arbuscular mycorrhizal fungi and Azospirillum brasilense 丛枝菌根真菌和巴西偶氮螺旋菌接种‘甜Nufar’罗勒的生长
Q3 Agricultural and Biological Sciences Pub Date : 2022-12-01 DOI: 10.5154/r.rchsh.2022.03.005
Homero González-Gómez, P. Juárez-López, E. Quiñones-Aguilar, G. Rincón‐Enríquez, I. Alia-Tejacal, J. A. Ramírez-Trujillo, V. López‐Martínez, O. Villegas-Torres, Cid Aguilar-Carpio
The use of beneficial microorganisms in agriculture can increase the productivity of agricultural crops; however, there are few studies that consider aromatic plants, such as basil (Ocimum basilicum L.). The aim of this study was to evaluate the growth of 'Sweet Nufar' basil inoculated with arbuscular mycorrhizal fungi (AMF) and Azospirillum brasilense. The treatments tested were: 1) without microorganisms (control), 2) Rizhophagus intraradices, 3) Funneliformis mosseae, 4) AMF consortium, 5) Azospirillum brasilense, 6) R. intraradices + A. brasilense and 7) without microorganisms and irrigated with 100 % Steiner's nutrient solution. Treatments 1 to 6 were irrigated with the same nutrient solution, but at 50 %. Fifty days after transplanting, growth variables, mineral concentration, colonization percentage and number of AMF spores were recorded. The AMF and A. brasilense bacterium increased the fresh biomass weight and leaf area of 'Nufar' basil, which was associated with the highest concentration of nitrogen and potassium in the plant tissue. This implies that the microorganisms evaluated increase the development and growth of basil grown with reduced levels of mineral nutrition, which represents a sustainable alternative in the agronomic management of this crop under greenhouse conditions.
在农业中使用有益微生物可以提高农业作物的生产力;然而,很少有研究考虑芳香植物,如罗勒(Ocimum basilicum L.)。本研究的目的是评估用丛枝菌根真菌(AMF)和巴西固氮螺菌(Azospirillum brasilense)接种的“Sweet Nufar”罗勒的生长。试验的处理是:1)无微生物(对照),2)根内Rizophagus,3)Funneliformis mosseae,4)AMF联合体,5)巴西氮螺菌,6)根内R.+A.brasilense和7)无微生物并用100%Steiner营养液灌溉。处理1至6用相同的营养液灌溉,但灌溉量为50%。移植后50天,记录生长变量、矿物质浓度、定殖百分比和AMF孢子数。AMF和A.brasilense细菌增加了“Nufar”罗勒的新鲜生物量和叶面积,这与植物组织中氮和钾的最高浓度有关。这意味着所评估的微生物增加了罗勒的发育和生长,而罗勒的矿物质营养水平降低,这代表了在温室条件下对该作物进行农艺管理的可持续替代方案。
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引用次数: 0
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Revista Chapingo, Serie Horticultura
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