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Investigation of Callipyge Gene Polymorphism in Sheep Breed 绵羊品种Callipyge基因多态性研究
Pub Date : 2022-11-17 DOI: 10.53433/yyufbed.1145441
Melike Kavuzkoz, Koray Kirikci
Callipyge is a candidate gene with a significant effect on meat quality and growth in sheep. The aim of this study is to investigate the polymorphism of the Callipyge gene (CLPG) in Akkaraman breed. In the study, DNAs obtained from blood samples of 50 Akkaraman sheep were used as study material. The CLPG/Faq1 polymorphism was examined in all individuals using the method PCR-RFLP. Only A allele and AA genotype were detected after PCR-RFLP procedure. This is the first study in which Callipyge gene polymorphism was investigated in Akkaraman breed, and the findings show that Akkaraman breed has a monomorphic structure in terms of Callipyge/Faq1 polymorphism.
Callipyge基因是对绵羊肉质和生长有重要影响的候选基因。本研究旨在研究阿卡拉曼品种Callipyge基因(CLPG)的多态性。在这项研究中,从50只阿卡拉曼羊的血液样本中获得的dna被用作研究材料。使用PCR-RFLP方法检测所有个体的CLPG/Faq1多态性。PCR-RFLP方法仅检测到A等位基因和AA基因型。本研究首次对Akkaraman品种的Callipyge基因多态性进行了研究,结果表明Akkaraman品种在Callipyge/Faq1多态性方面具有单态结构。
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引用次数: 0
WHAM Ha Verileri Kullanılarak NGC7822 (Sh2-171) HII Bölgesinin İncelenmesi
Pub Date : 2022-11-14 DOI: 10.53433/yyufbed.1129415
M. Şahan
Bu çalışmada, Wisconsin H-Alpha Mapper (WHAM) verilerini kullanılarak NGC7822 (Sh2-171, W1) HII bölgesinin çevresindeki 4x4’lik bölgenin (l=116º.1 < l < 120°.03 , 3°.39
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引用次数: 0
The Web Structure and Morphology of the Funnel Web Spider of Agelena orientalis (Araneae: Agelenidae: Ageleninae) 东方艾格丽娜漏斗网蜘蛛的网结构与形态(蜘蛛目:艾格丽娜科:艾格丽娜科)
Pub Date : 2022-11-09 DOI: 10.53433/yyufbed.1130015
Nazife YİĞİT KAYHAN, İlkay Çorak Öcal, Ümmügülsüm Hanife Aktaş
The web-spinning activity feature of spiders has affected human beings since ancient times. But not all spiders spin webs. Spiders owe their web-spinning abilities to their possession of spinning apparatus. The spinning apparatus consists of silk glands producing silk secretion and spinnerets attached to these glands. The spinnerets and spigots are used for extracting the silk and processing it by turning it into silk fibers. Although spider webs attract a lot of attention, there are not many studies on spinning apparatus. In this study, the web structure of the funnel web spider Agelena orientalis (C.L. Koch, 1837), which has a very remarkable network structure, and the structural organization of the web-spinning apparatus were studied using scanning electron microscopy (SEM). In our study, the web structure, arrangement of spinnerets and spigots of A. orientalis were shown and the obtained data were discussed in the light of the literature.
蜘蛛的织网活动特征自古以来就影响着人类。但不是所有的蜘蛛都结网。蜘蛛的织网能力归功于它们拥有纺丝装置。纺丝装置由分泌丝的丝腺和附着在这些丝腺上的吐丝器组成。吐丝器和喷丝器用于提取蚕丝并将其加工成蚕丝纤维。虽然人们对蜘蛛网的研究非常关注,但对织网装置的研究并不多。本研究利用扫描电子显微镜(SEM)研究了具有非常显著的网状结构的漏斗网蜘蛛Agelena orientalis (C.L. Koch, 1837)的网状结构和结网器的结构组织。本研究展示了东方剑麻的网结构、喷丝器和喷丝器的排列,并结合文献对所获得的数据进行了讨论。
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引用次数: 0
Extraction of Phenolic Compounds from Green Walnut by Ultrasound Assisted Extraction 超声辅助提取青核桃中酚类化合物的研究
Pub Date : 2022-11-07 DOI: 10.53433/yyufbed.1150133
Serdar Uğurlu, E. Bakkalbaşı
Bu çalışmada, yeşil cevizden fenolik bileşiklerin ekstraksiyonuna iki farklı yöntemin etkisi araştırılmıştır. Fenolik bileşikler yeşil cevizden konvansiyonel yöntemle (120, 240, 360 480, 720, 960 ve 1200 dakika) ve ultrason destekli ekstraksiyon (%25, 50, 75 ve 100 genlik; 10, 20, 30, 40, 50 ve 60 dakika) ile ekstrakte edilmiştir. HPLC analizi ile yeşil ceviz ekstraktlarında bazı fenolik asitler (gallik asit, neoklorojenik asit), flavonol (rutin) ve juglon tespit edilmiştir. Konvansiyonel yöntemde toplam fenolik içerik 240 dakika sonra 158.55 mg GAE/kg olarak belirlendi. Ultrason destekli ekstraksiyonda ise toplam fenolik içerik, 60 dakika sonunda %100 genlikte 156.80 mg GAE/kg olarak ölçülmüştür. Konvansiyonel ekstraksiyonda ise toplam fenolik içerik, 1200 dakika sonunda 1478.15 mg GAE/kg olarak ölçülmüştür. Ekstraksiyon süresi artırıldığında konvansiyonel ekstraksiyon etkili olmuştur. En iyi ekstraksiyon verimi, %100 ultrason genliğinde elde edilse de 50. dakikadan sonra fenolik bileşiklerdeki kayıplar nedeniyle ekstraksiyon süresi uzatılamamakta ve uzun sürmesine karşılık bozunma olmadığı için konvansiyonel ekstraksiyonda daha yüksek fenolik bileşik değerlerine ulaşılmaktadır.
本研究调查了两种不同方法对从青核桃中提取酚类化合物的影响。采用传统方法(120、240、360、360、480、720、960 和 1200 分钟)和超声辅助萃取法(振幅分别为 25、50、75 和 100%;10、20、30、40、50 和 60 分钟)从青核桃中提取酚类化合物。通过 HPLC 分析测定了青核桃提取物中的一些酚酸(没食子酸、新绿原酸)、黄酮醇(芦丁)和黄酮。在传统方法中,240 分钟后测定的总酚含量为 158.55 mg GAE/kg。在超声波辅助萃取法中,60 分钟后,在 100%振幅下测得总酚含量为 156.80 毫克 GAE/公斤。在常规萃取中,1200 分钟后测得总酚含量为 1478.15 毫克 GAE/公斤。萃取时间越长,传统萃取的效果越好。虽然在超声波振幅为 100%时萃取效率最高,但由于酚类化合物在 50 分钟后损失殆尽,因此不能延长萃取时间,而常规萃取的酚类化合物值更高,因为尽管萃取时间较长,但酚类化合物没有降解。
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引用次数: 2
Antioxidant Activity of lyophilized water extract of aerial parts of Astragalus gummifer (Günizer) 黄芪地上部位冻干水提取物的抗氧化活性研究
Pub Date : 2022-10-20 DOI: 10.53433/yyufbed.1107954
Hatice Kiziltaş
Studies shown an inverse correlation among age-related illnesses like coronary heart disease and cancer and intake of fruit and vegetable. Given the probable health benefits of natural antioxidants from plants, research on them has increased. The largest genus of the Fabaceae family is Astragalus L. and 224 endemic species were identified in the flora of Turkey. Since Astragalus species are plants grown in many regions of Turkey and used in traditional medicine, it is scientifically important to evaluate the biological activities of these species. In this study, the antioxidant properties of Astragalus gummifer were investigated. The antioxidant capacity of the lyophilized water extract obtained from the aerial parts was evaluated by ferric ions (Fe3+) reduction, KUPRAK, FRAP, DPPH• and ABTS+• scavenging antioxidant methods. This research provides a basis for further phytochemical research on Astragalus gummifer.
研究表明,冠心病和癌症等与年龄有关的疾病与水果和蔬菜的摄入量呈负相关。鉴于来自植物的天然抗氧化剂可能对健康有益,对它们的研究越来越多。豆科植物中最大的属是黄芪,在土耳其植物区系中鉴定出特有种224种。由于黄芪属植物生长在土耳其的许多地区并用于传统医学,因此评估这些物种的生物活性具有重要的科学意义。本研究对黄芪的抗氧化性能进行了研究。采用铁离子还原法(Fe3+)、KUPRAK法、FRAP法、DPPH•法和ABTS+•法对冻干水提取物的抗氧化能力进行了评价。本研究为进一步研究黄芪的植物化学成分提供了基础。
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引用次数: 0
Comparison of Whole and Section Otolith Methods for Age Determination of Carp (Cyprinus carpio) 鲤鱼全耳石法与切片耳石法测定年龄的比较
Pub Date : 2022-10-19 DOI: 10.53433/yyufbed.1129009
Aykut Aydın, N. Polat, S. Saygın, M. Özpiçak, S. Yılmaz
This study aims to determine the reliability of age determination by comparing whole and section otoliths of Cyprinus carpio species living in Altınkaya Dam Lake (ADL) and Bafra Fish Lakes (BFL). Fish samples (117 individuals) were obtained between 2017-2018, and the total lengths of each fish were measured (±0.1 cm) and their weights (±0.01 g) were weighed. Ages were determined from the distal surfaces of all otoliths (asteriscus) and transversal sections were taken. Percent agreement, average percent error and coefficient of variation were calculated. In the population, total lengths ranged between 17.8-69.3 cm and weights ranged between 67.57-5 412 g. The ages read in whole and section otoliths were determined as 2-6, 2-7 in ADL and 1-8,0-7 in BFL, respectively. It is the section otolith method with the highest percent agreement (75.43% and 71.67%, respectively), the lowest mean percent error (3.89; 3.98) and coefficient of variation (5.11; 5.29) for ADL and BFL. For age determination of C.carpio species in both localities, it has been determined that the age readings obtained by the section otolith method provide more reliable results than the whole otolith method.
本研究旨在通过比较Altınkaya坝湖(ADL)和Bafra鱼湖(BFL)鲤科鲤(Cyprinus carpio)的整体和剖面耳石,确定年龄测定的可靠性。在2017-2018年间获得了117条鱼的样本,并测量了每条鱼的总长度(±0.1 cm)和重量(±0.01 g)。从所有耳石(星形)远端表面测定年龄,并取横切面。计算一致性百分比、平均误差百分比和变异系数。总体长在17.8 ~ 69.3 cm之间,体重在67.57 ~ 5 412 g之间。整体和剖面耳石年龄分别为ADL的2-6、2-7岁和BFL的1-8、0-7岁。剖面耳石法的一致性最高(分别为75.43%和71.67%),平均误差率最低(3.89;3.98)和变异系数(5.11;ADL和BFL为5.29)。对于这两个地点的卡皮蛇种的年龄测定,已经确定用剖面耳石法获得的年龄读数比用整体耳石法获得的结果更可靠。
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引用次数: 0
Energy and Exergy Analysis into Using of Air and Ground Source Heat Pumps for Kastamonu Province 卡斯塔莫努省空气源热泵和地源热泵的能量和火用分析
Pub Date : 2022-10-17 DOI: 10.53433/yyufbed.1112972
M. Gürdal
This study includes energy and exergy analysis if air and ground source heat pumps are used for residential heating in Kastamonu province. The lowest air and soil temperatures measured in Kastamonu between December and March between 2000-2021 were taken into account. R134a, R407c, R410a and R500 are used as refrigerants for the heating process that will benefit from residential heating. In the study discussed, the ambient temperature to be heated was chosen the same for each heat pump, the heating performance coefficient (COP) and the second law efficiency were calculated and the results were compared. As a result, it has been observed that ground source heat pump provides better performance than air source heat pumps for all refrigerants in Kastamonu conditions. On the other hand, it was concluded that air source heat pump has higher second law efficiency than ground source heat pumps. In addition, it has been determined that the R500 refrigerant provides a better performance than other refrigerants.
本研究包括能源和火用分析,如果空气和地源热泵用于住宅供暖在Kastamonu省。考虑了2000年至2021年12月至3月期间在Kastamonu测量的最低空气和土壤温度。R134a, R407c, R410a和R500作为加热过程中的制冷剂,将受益于住宅供暖。在本研究中,每台热泵选择相同的被加热环境温度,计算其采暖性能系数(COP)和第二定律效率,并对结果进行比较。因此,在Kastamonu条件下,地源热泵对所有制冷剂的性能都优于空气源热泵。另一方面,空气源热泵比地源热泵具有更高的第二定律效率。同时,R500制冷剂的性能优于其他制冷剂。
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引用次数: 0
Donmuş Çözünmüş Kanın Farklı Değerlerde Santrifüjünün DNA İzolasyonu Üzerine Etkileri
Pub Date : 2022-10-17 DOI: 10.53433/yyufbed.1130525
Mevlut Arslan
DNA isolation from blood is a commonly used application to obtain nDNA and mtDNA. It was previously shown that DNA isolation could be performed from the pellet obtained after centrifugation of freeze-thawed blood (FTB), and this pretreatment had constructive results on DNA isolation. However, which centrifugation levels can be used for this pretreatment and their effects are unknown. The aim of the study was to determine appropriate centrifugation levels for this pretreatment and show their effects on isolated DNA. For this purpose DNA isolations were carried out from both pellet and supernatant obtained by centrifugation at different levels of FTB. Then, spectrophotometric, gel electrophoresis, and real-time PCR analyses were performed in the isolated DNA samples. As a result, centrifugation of FTB at 5 000 ×g for 2 min or over let genetic material to pellet completely, which enable to obtain higher DNA yield. Real-time PCR results showed that mtDNA/nDNA ratios did not change in the isolated DNA samples which were performed from pellets obtained by defined centrifugation levels whereas isolated DNA integrity decreased. To conclude, centrifugation at 5 000 ×g for 2 min or over can be used to harvest and wash genetic material found in FTB before DNA isolations.
从血液中分离DNA是获得nDNA和mtDNA的常用方法。先前的研究表明,从冻融血液(FTB)离心后获得的颗粒中可以分离DNA,并且这种预处理对DNA分离具有建设性的结果。然而,哪种离心水平可以用于这种预处理及其效果是未知的。本研究的目的是确定适当的离心水平,这种预处理和显示其对分离的DNA的影响。为此,从不同水平的FTB离心得到的颗粒和上清液中进行DNA分离。然后对分离的DNA样本进行分光光度、凝胶电泳和实时PCR分析。因此,FTB在5 000 ×g下离心2分钟或更长时间,使遗传物质完全成球,从而获得更高的DNA产量。实时荧光定量PCR结果显示,分离DNA样品的mtDNA/nDNA比率没有变化,而分离DNA的完整性降低。总之,在5 000 ×g离心2分钟或更长时间,可用于在分离DNA之前收集和洗涤在FTB中发现的遗传物质。
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引用次数: 0
Regional Analysis of Electricity Energy Produced from Animal Manure Sourced Biogas in Turkey 土耳其动物粪便沼气发电的区域分析
Pub Date : 2022-10-11 DOI: 10.53433/yyufbed.1123110
S. Ünvar
In this study, it was tried to determine the biogas and electricity production amounts of Turkey's animal manure (bovine, ovine and poultry) by using the 2021 data of the Turkish Statistical Institute (TSI) and it was determined how much of the electrical energy our country could meet for 2021. Biogas and electrical energy production amounts were calculated based on the total number of animals in Turkey. In this context, our country's biogas production from animal waste for 2021 has been determined as 15.894×106 m3/year and electrical energy production as 28.609×106 kWh/year. As a result of the calculations, it has been determined that the first three regions with the highest annual biogas amounts are the Aegean Region (3.889×106 m3/year), the Central Anatolia Region (2.701×106 m3/year) and the Mediterranean Region (2.533×106 m3/year). It has been determined that the first three regions with the highest annual electricity production amounts are Aegean Region (7.000×106 kWh/year), Central Anatolia Region (4.862×106 kWh/year) and Mediterranean Region (4.559×106 kWh/year). Considering that the total electricity consumption of our country in 2021 is 329.634×106 kWh, it has been determined that 8.67% of the annual energy need can be met by using biogas energy if there are biogas facilities in each province.
在本研究中,通过使用土耳其统计研究所(TSI)的2021年数据,试图确定土耳其动物粪便(牛、羊和家禽)的沼气和发电量,并确定了我国在2021年可以满足多少电能。沼气和电能产量是根据土耳其动物总数计算的。在此背景下,我国2021年动物粪便沼气产量确定为15.894×106 m3/年,电能产量确定为28.609×106 kWh/年。根据计算,已确定年沼气量最高的前三个地区是爱琴海地区(3.889×106立方米/年)、安纳托利亚中部地区(2.701×106立方米/年)和地中海地区(2.533×106立方米/年)。据确定,年发电量最高的前三个地区是爱琴海地区(7.000×106 kWh/年)、安纳托利亚中部地区(4.862×106 kWh/年)和地中海地区(4.559×106 kWh/年)。考虑到2021年我国总用电量为329.634×106千瓦时,确定如果各省都有沼气设施,利用沼气能可满足全年能源需求的8.67%。
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引用次数: 1
An Overview of the Seismicity of Lake Van Basin and its Surrounding Area 凡湖盆地及其周边地区地震活动性综述
Pub Date : 2022-10-07 DOI: 10.53433/yyufbed.1138320
H. Alkan, Mustafa Toker, İ. Akkaya
In the scope of this study, the findings obtained from the previous studies using the main shocks with their aftershocks that occurred especially in the recent past are evaluated for the Van Lake Basin. Using the different seismological parameters, the results of these previous studies are compiled to reveal the future earthquake hazard. In the region, many destructive earthquakes with magnitudes greater than 5.0 occurred in the instrumental and historical periods. Finally, two destructive/damaging earthquakes that occurred around the city center of Van on October 23, 2011 (Mw=7.1) and 9 November 2011 (Mw=5.8) showed that the region has active fault mechanisms with different characteristics. As a result, the earthquake hazard evaluation and earthquake forecasting in the near future show higher seismic risk in the east of Lake Van, especially around Çaldıran, Muradiye, Özalp, Saray, and Erciş than in other regions. In addition to seismic risk analyses, the evaluation of the Van Lake Basin and its surroundings, in terms of soil engineering properties and soil-structure interaction, using different parameters based on both geophysical methods and structural investigations, were also examined in this study.
在本研究的范围内,利用主震及其余震(特别是最近发生的余震)对Van Lake盆地进行了评估。利用不同的地震参数,对以往的研究结果进行整理,以揭示未来的地震危险性。在仪器和历史时期,该地区发生了许多震级大于5.0级的破坏性地震。2011年10月23日(Mw=7.1)和2011年11月9日(Mw=5.8)在Van市中心附近发生的两次破坏性/破坏性地震表明该地区存在不同特征的活动断层机制。因此,近期地震危险性评价和地震预报结果表明,凡湖东部,特别是Çaldıran、Muradiye、Özalp、Saray和erciu附近的地震危险性高于其他地区。除了地震风险分析外,本研究还基于地球物理方法和结构调查,在不同参数下对Van Lake盆地及其周边地区进行了土壤工程性质和土-结构相互作用的评价。
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引用次数: 0
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Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi
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