Pub Date : 2018-11-01DOI: 10.16875/stem.2018.19.4.109
Spring, Ryan, Suarez, Gerard
{"title":"How and Why to Teach English Metaphors to Japanese Learners Through Visual Media: With Practical Examples From Steven Universe","authors":"Spring, Ryan, Suarez, Gerard","doi":"10.16875/stem.2018.19.4.109","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.109","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"317 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75454945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.16875/stem.2018.19.4.27
고성란
{"title":"An Analysis of English Vocabulary Based on Corpora From the Korean and American Versions of The Good Doctor","authors":"고성란","doi":"10.16875/stem.2018.19.4.27","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.27","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"182 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75674630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.16875/stem.2018.19.4.149
Nayu Kim, 이희철
{"title":"A Study on the Validity of the English Learning Style Scale for Korean High School Students in a Native Teacher’s Class Based on Multimedia","authors":"Nayu Kim, 이희철","doi":"10.16875/stem.2018.19.4.149","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.149","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90446850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.16875/STEM.2018.19.4.127
Hye-Kyung Kang
,
，
{"title":"A Case Study on the Contents Creation of Online Lectures to Promote Interest and Concentration of Learners","authors":"Hye-Kyung Kang","doi":"10.16875/STEM.2018.19.4.127","DOIUrl":"https://doi.org/10.16875/STEM.2018.19.4.127","url":null,"abstract":",","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"114 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88126262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.16875/stem.2018.19.4.45
Andrea Lee
{"title":"Exploring Intercultural Communication Themes in English Classes Through the Movie Outsourced","authors":"Andrea Lee","doi":"10.16875/stem.2018.19.4.45","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.45","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"283 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75855151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.16875/stem.2018.19.4.71
Lee, Jin Hyung
{"title":"The Effect of Inferencing Tasks on Low-Proficiency Korean EFL Learners’ Learning Vocabulary From Movies","authors":"Lee, Jin Hyung","doi":"10.16875/stem.2018.19.4.71","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.71","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"71 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72453403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.16875/stem.2018.19.4.1
Kim, Kitai
{"title":"The Effectiveness of Using Films and Documentaries in the EAP Classroom to Teach Contemporary Political Discourse","authors":"Kim, Kitai","doi":"10.16875/stem.2018.19.4.1","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.1","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85110802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-28DOI: 10.2174/1876893801805010001
A. Kamal, Deded Yudha Pranatha, Waluyo Sugito, F. Rahman, Eka Susanto, S. Mariya, Wei Ming Chen
Osteosarcoma cancer stem cells (CSCs) are defined as a subpopulation of osteosarcoma cells, which have the ability of self-renewal, proliferation and differentiation. This study aimed to identify CSCs from human osteosarcomain vitro.Osteosarcoma CSCs were isolated and cultured with sphere-forming assay technique on an ultra-low well attachment surface plate. After sarcosphere colonies were formed, we conducted reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the expression of genes of embryonic stem cells such asNANOG, Oct3/4, STAT3 and gene of MSC CD133. Immunofluorescence analysis (IFA) of alkaline phosphatase (ALP), osteocalcin, and CD 133 was also performed to see the expression of osteosarcoma CSC surface protein with immuno-enzymatic staining principle. We also performed alizarin red staining to evaluate calcification in osteosarcoma CSCs.The culture sphere-of the osteosarcoma cells showed three dimension round shaped colonies (sarcospheres) in slightly hypoxicand serum free condition which was not attached to the substrate with tight density. RT-PCR demonstrated that sarcospheres expressed genes which encodeNANOG, Oct3/4 STAT 3, but not for CD 133. IFA showed positive protein expression of ALP, osteocalcin and CD 133 which was moderate, strong, and weak positive respectively. Sarcospheres also had a positive reaction toward alizarin red staining.Osteosarcoma CSCs could be isolated from human osteosarcoma by sphere-forming assay technique and characterized by the expression of genes of embryonic stem cells,such asNANOG, Oct3/4, STAT3 and IFA of ALP, osteocalcin, and CD 133.
骨肉瘤癌症干细胞(Osteosarcoma cancer stem cells, CSCs)是骨肉瘤细胞的一个亚群，具有自我更新、增殖和分化的能力。本研究旨在体外鉴定人骨肉瘤干细胞。采用超低孔贴壁法分离培养骨肉瘤CSCs。肌球集落形成后，我们采用逆转录聚合酶链反应(RT-PCR)检测胚胎干细胞nanog、Oct3/4、STAT3等基因和MSC CD133基因的表达。采用免疫-酶染色法对碱性磷酸酶(ALP)、骨钙素和cd133进行免疫荧光分析(IFA)，观察骨肉瘤CSC表面蛋白的表达情况。我们也用茜素红染色评价骨肉瘤CSCs的钙化。骨肉瘤细胞培养球在微缺氧和无血清条件下呈三维圆形集落(肌球)，不致密附着于底物。RT-PCR结果显示，肌球表达编码denanog、Oct3/4 STAT 3的基因，但不表达cd133的基因。IFA显示ALP、骨钙素和cd133蛋白表达阳性，分别为中度、强、弱阳性。肌球对茜素红染色也有阳性反应。利用成球实验技术可从人骨肉瘤中分离到骨肉瘤CSCs，其特征是胚胎干细胞的nanog、Oct3/4、STAT3和ALP、骨钙素、cd133等基因的表达。
{"title":"Isolation, Culture and Characterization of Cancer Stem Cells from Primary Osteosarcoma","authors":"A. Kamal, Deded Yudha Pranatha, Waluyo Sugito, F. Rahman, Eka Susanto, S. Mariya, Wei Ming Chen","doi":"10.2174/1876893801805010001","DOIUrl":"https://doi.org/10.2174/1876893801805010001","url":null,"abstract":"Osteosarcoma cancer stem cells (CSCs) are defined as a subpopulation of osteosarcoma cells, which have the ability of self-renewal, proliferation and differentiation. This study aimed to identify CSCs from human osteosarcomain vitro.Osteosarcoma CSCs were isolated and cultured with sphere-forming assay technique on an ultra-low well attachment surface plate. After sarcosphere colonies were formed, we conducted reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the expression of genes of embryonic stem cells such asNANOG, Oct3/4, STAT3 and gene of MSC CD133. Immunofluorescence analysis (IFA) of alkaline phosphatase (ALP), osteocalcin, and CD 133 was also performed to see the expression of osteosarcoma CSC surface protein with immuno-enzymatic staining principle. We also performed alizarin red staining to evaluate calcification in osteosarcoma CSCs.The culture sphere-of the osteosarcoma cells showed three dimension round shaped colonies (sarcospheres) in slightly hypoxicand serum free condition which was not attached to the substrate with tight density. RT-PCR demonstrated that sarcospheres expressed genes which encodeNANOG, Oct3/4 STAT 3, but not for CD 133. IFA showed positive protein expression of ALP, osteocalcin and CD 133 which was moderate, strong, and weak positive respectively. Sarcospheres also had a positive reaction toward alizarin red staining.Osteosarcoma CSCs could be isolated from human osteosarcoma by sphere-forming assay technique and characterized by the expression of genes of embryonic stem cells,such asNANOG, Oct3/4, STAT3 and IFA of ALP, osteocalcin, and CD 133.","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48550834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-01DOI: 10.16875/stem.2018.19.3.45
서은미
The purpose of this paper is to show the effect of cinema-based learning for EFL college students. Constructive learning theory maintains that...
本文旨在探讨电影学习对大学生英语学习的影响。建构性学习理论认为……
{"title":"영화를 기반으로 한 영어 향상 방안","authors":"서은미","doi":"10.16875/stem.2018.19.3.45","DOIUrl":"https://doi.org/10.16875/stem.2018.19.3.45","url":null,"abstract":"The purpose of this paper is to show the effect of cinema-based learning for EFL college students. Constructive learning theory maintains that...","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"6 1","pages":"45-64"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80322169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：