Pub Date : 2019-04-15DOI: 10.37855/jah.2019.v21i01.02
B. A. Harsojuwono, S. Mulyani, I. W. Arnata
The production of bio-plastics from modified cassava starch and konjac glucomannan had been widely and dependently developed but still demonstrated characteristics that do not meet international quality standards. Therefore the key question for further exploration was to improve the protocol so as to develop a composite bio-plastic using the aforementioned materials. This study aimed to determine the ratio of modified cassava starch and konjac glucomannan and the concentration of acetic acid solution required to produce bioplastic composites with the best characteristics. This study followed a Factorial Randomized Block Design with two factors. Factor I was the ratio of the modified cassava starch and konjac glucomannan, and consisted of 5 levels namely 100:0, 75:25, 50:50, 25:75 and 0: 100. The factor II was the concentration of acetic acid which consisted of 5 levels, viz., 0, 0.5, 1.0, 1.5 and 2.0 %. Each treatment combination was grouped into 4 blocks based on the processing time of making bio-plastic composites, resulting in an altogether 100 experimental units. The data obtained were subjected to analysis of variance followed by Duncan’s multiple comparison tests. The results showed that the ratio of the modified cassava starch and konjac glucomannan, the concentration of acetic acid and its interaction had a very significant effect on the tensile strength, elongation at break, Young’s modulus, swelling and the degradation time of bio-plastic composites. The ratio of the modified cassava starch:konjac glucomannan :: 75:25 with supplemented with 1 % acetic acid produced the best bio-plastic composites with the desired characteristics viz., tensile strength of 1997.40 MPa, elongation at break of 8.90 %, Young’s modulus of 22442.70 MPa, swelling of 10.40 % and the degradation time of 6.33 days. The surface profile of bio-plastic composites in longitudinal appearance displayed presence of regular waveforms along with air cavities or regular pores. Bio-plastic composite profile in transverse appearance revealed arrangement of fibers in the form of regular nets and smooth cross links. These bio-plastic composites contained -OH, -CH, -C-C, -C=C, -NH and -C=O functional groups.
{"title":"Characteristics of bio-plastic composites from the modified cassava starch and konjac glucomannan","authors":"B. A. Harsojuwono, S. Mulyani, I. W. Arnata","doi":"10.37855/jah.2019.v21i01.02","DOIUrl":"https://doi.org/10.37855/jah.2019.v21i01.02","url":null,"abstract":"The production of bio-plastics from modified cassava starch and konjac glucomannan had been widely and dependently developed but still demonstrated characteristics that do not meet international quality standards. Therefore the key question for further exploration was to improve the protocol so as to develop a composite bio-plastic using the aforementioned materials. This study aimed to determine the ratio of modified cassava starch and konjac glucomannan and the concentration of acetic acid solution required to produce bioplastic composites with the best characteristics. This study followed a Factorial Randomized Block Design with two factors. Factor I was the ratio of the modified cassava starch and konjac glucomannan, and consisted of 5 levels namely 100:0, 75:25, 50:50, 25:75 and 0: 100. The factor II was the concentration of acetic acid which consisted of 5 levels, viz., 0, 0.5, 1.0, 1.5 and 2.0 %. Each treatment combination was grouped into 4 blocks based on the processing time of making bio-plastic composites, resulting in an altogether 100 experimental units. The data obtained were subjected to analysis of variance followed by Duncan’s multiple comparison tests. The results showed that the ratio of the modified cassava starch and konjac glucomannan, the concentration of acetic acid and its interaction had a very significant effect on the tensile strength, elongation at break, Young’s modulus, swelling and the degradation time of bio-plastic composites. The ratio of the modified cassava starch:konjac glucomannan :: 75:25 with supplemented with 1 % acetic acid produced the best bio-plastic composites with the desired characteristics viz., tensile strength of 1997.40 MPa, elongation at break of 8.90 %, Young’s modulus of 22442.70 MPa, swelling of 10.40 % and the degradation time of 6.33 days. The surface profile of bio-plastic composites in longitudinal appearance displayed presence of regular waveforms along with air cavities or regular pores. Bio-plastic composite profile in transverse appearance revealed arrangement of fibers in the form of regular nets and smooth cross links. These bio-plastic composites contained -OH, -CH, -C-C, -C=C, -NH and -C=O functional groups.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44011050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-15DOI: 10.37855/jah.2019.v21i01.10
Jalal Omidi, Samaneh Abdolmohammadi, A. Hatamzadeh, A. Mahboub khomami
Peanut shells, leftover as wastes from peanut planting, in combination with considerable amount of compost can be used as an alternative source to replace with peat in cultivation of ornamental plants. In this experiment, five treatments in a completely randomized design with three replications were conducted outdoors at Lahijan ornamental plants research station. The treatments comprised of 2 peat + 1 perlite + 0 Shells Peanut composts (control), 1.5 Peat + 1 perlite + 0.5 Shells peanut compost, 1 peat + 1 perlite + 1 Shells Peanut composts, 0.5 Peat + 1 perlite + 1.5 peanut shells compost, 0 peat + 1 perlite + 2 peanut shells compost. The estimated parameters included number of flowers, plant height, fresh and dry weight of canopy, root length, fresh and dry weight of roots, and physical and chemical properties of substrates on Viola spp. that were cultivated on them. The results showed that replacement with peanut shells compost had a significant effect on number of flowers, plant height, dry weight of canopy and fresh as well as dry weight of roots in comparison to control. Increasing levels of peanut shells compost caused reduction in bulk density and an increase in total porosity, water capacity and air fill porosity.
{"title":"Peanut shell compost as an alternative to peat and its effect on growth indices and properties of Viola spp. grown outdoors","authors":"Jalal Omidi, Samaneh Abdolmohammadi, A. Hatamzadeh, A. Mahboub khomami","doi":"10.37855/jah.2019.v21i01.10","DOIUrl":"https://doi.org/10.37855/jah.2019.v21i01.10","url":null,"abstract":"Peanut shells, leftover as wastes from peanut planting, in combination with considerable amount of compost can be used as an alternative source to replace with peat in cultivation of ornamental plants. In this experiment, five treatments in a completely randomized design with three replications were conducted outdoors at Lahijan ornamental plants research station. The treatments comprised of 2 peat + 1 perlite + 0 Shells Peanut composts (control), 1.5 Peat + 1 perlite + 0.5 Shells peanut compost, 1 peat + 1 perlite + 1 Shells Peanut composts, 0.5 Peat + 1 perlite + 1.5 peanut shells compost, 0 peat + 1 perlite + 2 peanut shells compost. The estimated parameters included number of flowers, plant height, fresh and dry weight of canopy, root length, fresh and dry weight of roots, and physical and chemical properties of substrates on Viola spp. that were cultivated on them. The results showed that replacement with peanut shells compost had a significant effect on number of flowers, plant height, dry weight of canopy and fresh as well as dry weight of roots in comparison to control. Increasing levels of peanut shells compost caused reduction in bulk density and an increase in total porosity, water capacity and air fill porosity.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42315955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-15DOI: 10.37855/jah.2019.v21i01.12
P. Tripathi, G. Karunakaran
Avocado is propagated by seed in India. The plants produced from seeds are not uniform and take long time to start bearing and fruit quality is unreliable. The propagation of superior clones of avocado by vegetative method is essential to avoid these problems. Thus a study was conducted to standardize the method and time of propagation of avocado through vegetative means. There were four methods of multiplication namely veneer grafting, cleft grafting, Tbudding and patch budding which were performed in all the months of the year at CHES (IIHR), Chettalli. After 90 days of grafting , mean percent success was highest in cleft grafting (32.5%) while lower mean success was observed in veneer grafting (8.3%), Tbudding (7.5%)and patching budding (5.8%). Among different months, highest mean success was recorded in month of September (32.5%) followed by 22.5 per cent in the month of March and August. Among different combinations of months and propagation methods, highest success (70 %) was recorded in cleft grafting performed in the month of September. This was closely followed by same method done in the month of March (60%). The success in cleft grafting is higher than other three methods in all 12 months. The growth of the scion was higher in grafting methods than budding methods. The percent transplantable seedlings after one year were highest (60%) in cleft grafting done in the month of September. It was concluded that cleft grafting in the month of September and March was found most suitable for the vegetative propagation of avocado under humid tropical conditions.
{"title":"Standardization of time and method of propagation in avocado","authors":"P. Tripathi, G. Karunakaran","doi":"10.37855/jah.2019.v21i01.12","DOIUrl":"https://doi.org/10.37855/jah.2019.v21i01.12","url":null,"abstract":"Avocado is propagated by seed in India. The plants produced from seeds are not uniform and take long time to start bearing and fruit quality is unreliable. The propagation of superior clones of avocado by vegetative method is essential to avoid these problems. Thus a study was conducted to standardize the method and time of propagation of avocado through vegetative means. There were four methods of multiplication namely veneer grafting, cleft grafting, Tbudding and patch budding which were performed in all the months of the year at CHES (IIHR), Chettalli. After 90 days of grafting , mean percent success was highest in cleft grafting (32.5%) while lower mean success was observed in veneer grafting (8.3%), Tbudding (7.5%)and patching budding (5.8%). Among different months, highest mean success was recorded in month of September (32.5%) followed by 22.5 per cent in the month of March and August. Among different combinations of months and propagation methods, highest success (70 %) was recorded in cleft grafting performed in the month of September. This was closely followed by same method done in the month of March (60%). The success in cleft grafting is higher than other three methods in all 12 months. The growth of the scion was higher in grafting methods than budding methods. The percent transplantable seedlings after one year were highest (60%) in cleft grafting done in the month of September. It was concluded that cleft grafting in the month of September and March was found most suitable for the vegetative propagation of avocado under humid tropical conditions.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44102924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-15DOI: 10.37855/jah.2019.v21i01.01
K. Krisanapook, P. Anusornpornpong, T. Havananda, Kietsuda Luengwilai
Basic information on the inflorescence and flower development of Thai aromatic dwarf coconut was developed. Histology of inflorescences of the Thai dwarf aromatic coconut, ‘Nam Hom’, was compared to that of a typical tall-type coconut. Both types had the same stages of inflorescence and flower development. ‘Nam Hom’ had the same or greater number of inflorescences than did the tall type at any of the developmental stages. During two years of reproductive development, floral morphogenesis took about one year, while sex determination occurred in 3-4 months before spadix opening. Despite the difference in number of bunches per year observed in the two coconut types, their inflorescence and flower development were not different in the length of time and in the time of occurrence.
{"title":"Inflorescence and flower development in Thai aromatic coconut","authors":"K. Krisanapook, P. Anusornpornpong, T. Havananda, Kietsuda Luengwilai","doi":"10.37855/jah.2019.v21i01.01","DOIUrl":"https://doi.org/10.37855/jah.2019.v21i01.01","url":null,"abstract":"Basic information on the inflorescence and flower development of Thai aromatic dwarf coconut was developed. Histology of inflorescences of the Thai dwarf aromatic coconut, ‘Nam Hom’, was compared to that of a typical tall-type coconut. Both types had the same stages of inflorescence and flower development. ‘Nam Hom’ had the same or greater number of inflorescences than did the tall type at any of the developmental stages. During two years of reproductive development, floral morphogenesis took about one year, while sex determination occurred in 3-4 months before spadix opening. Despite the difference in number of bunches per year observed in the two coconut types, their inflorescence and flower development were not different in the length of time and in the time of occurrence.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45860580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.37855/jah.2018.v20i03.36
L. Rajendran, G. Karthikeyan, T. Raguchander, R. Samiyappan
The wood degrading fungi Ganoderma lucidum (Leys) Karst causing Basal Stem Rot (BSR) in coconut plantation is the most destructive disease in Southern India. The control of this soil borne pathogen is particularly complex due to their occurrence in dynamic environment at the interface of the root with the soil. Hence, combinations of biocontrol agents differing in their mode of action especially with endophyte have been explored. The endophytic bacteria Bacillus subtilis EPC 5 from healthy coconut palm root showed high inhibition activity among fifty five isolated strains. Further, talc-based formulation incorporating Pseudomonas fluorescens Pf1 and Trichoderma viride Tv1 were developed and used individually, in combination to manage highly virulent, laccase producing soil borne pathogen. The result indicated that soil application of above mentioned antagonist in combination @ 100 g each enriched with organic substrate for four times per year, significantly reduced the disease severity with high nut yield at two different locations of disease endemic areas.
{"title":"Effect of endophytic bacteria Bacillus subtilis EPC 5 on basal stem rot disease spread and nut yield in coconut plantations","authors":"L. Rajendran, G. Karthikeyan, T. Raguchander, R. Samiyappan","doi":"10.37855/jah.2018.v20i03.36","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.36","url":null,"abstract":"The wood degrading fungi Ganoderma lucidum (Leys) Karst causing Basal Stem Rot (BSR) in coconut plantation is the most destructive disease in Southern India. The control of this soil borne pathogen is particularly complex due to their occurrence in dynamic environment at the interface of the root with the soil. Hence, combinations of biocontrol agents differing in their mode of action especially with endophyte have been explored. The endophytic bacteria Bacillus subtilis EPC 5 from healthy coconut palm root showed high inhibition activity among fifty five isolated strains. Further, talc-based formulation incorporating Pseudomonas fluorescens Pf1 and Trichoderma viride Tv1 were developed and used individually, in combination to manage highly virulent, laccase producing soil borne pathogen. The result indicated that soil application of above mentioned antagonist in combination @ 100 g each enriched with organic substrate for four times per year, significantly reduced the disease severity with high nut yield at two different locations of disease endemic areas.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43081363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.37855/jah.2018.v20i03.43
M. Khalaj, M. Kanani
Nutrition is the cornerstone of plant production. Here, efforts have been made to study the effect of different nutrient solutions from the Netherlands floriculture companies (S1; Schreurs, S2: Florist, and S3: Research Station for Floriculture and Greenhouse Vegetables(RSFGV) on two gerbera cultivars (‘Stanza’ and ‘Double Dutch’). Total chlorophyll index (SPAD), flower harvest per plant, flower stem height, disk diameter, total carbohydrate, lignin, and cell membrane stability were significantly affected by treatments. The highest number of cut flowers was harvested in RSFGV solution which was about 24% and 50% more than Florist and Schreurs solutions, respectively. Schreurs’s solution showed the best impact on cell membrane stability, total carbohydrate, and lignin production. Flowers stem height, disk diameter, and hemi-cellulose content were significantly increased by RSFGV solution. The cultivar ‘Double Dutch’ showed the highest cell membrane stability, total carbohydrate, hemi-cellulose, lignin, and vase life. Flower stem height was highest in the cultivar ‘Stanza’. The interaction of nutrient solution and cultivar affected the studied parameters significantly, and the highest vase life was obtained in the cultivar ‘Double Dutch’ fertigated with Schreurs solution (11.4 d). Results indicated that Schreurs and RSFGV solutions could be the proper solutions for producing high-quality cut gerberas commercially.
{"title":"Flower longevity and quality attributes of gerbera cut flower affected by different nutrient solutions","authors":"M. Khalaj, M. Kanani","doi":"10.37855/jah.2018.v20i03.43","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.43","url":null,"abstract":"Nutrition is the cornerstone of plant production. Here, efforts have been made to study the effect of different nutrient solutions from the Netherlands floriculture companies (S1; Schreurs, S2: Florist, and S3: Research Station for Floriculture and Greenhouse Vegetables(RSFGV) on two gerbera cultivars (‘Stanza’ and ‘Double Dutch’). Total chlorophyll index (SPAD), flower harvest per plant, flower stem height, disk diameter, total carbohydrate, lignin, and cell membrane stability were significantly affected by treatments. The highest number of cut flowers was harvested in RSFGV solution which was about 24% and 50% more than Florist and Schreurs solutions, respectively. Schreurs’s solution showed the best impact on cell membrane stability, total carbohydrate, and lignin production. Flowers stem height, disk diameter, and hemi-cellulose content were significantly increased by RSFGV solution. The cultivar ‘Double Dutch’ showed the highest cell membrane stability, total carbohydrate, hemi-cellulose, lignin, and vase life. Flower stem height was highest in the cultivar ‘Stanza’. The interaction of nutrient solution and cultivar affected the studied parameters significantly, and the highest vase life was obtained in the cultivar ‘Double Dutch’ fertigated with Schreurs solution (11.4 d). Results indicated that Schreurs and RSFGV solutions could be the proper solutions for producing high-quality cut gerberas commercially.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46777237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.37855/jah.2018.v20i03.33
P. Tsoulpha, S. Alexandri, M. Tsaktsira
Almond-leaved tree is one of the most commonly found native forest species in Greece, exhibiting valuable properties and thus suitable for multipurpose silviculture. Several critical factors were studied for the development of a successful micropropagation protocol of Pyrus spinosa juvenile trees. Newly immerged shoots of three-year-old plants, after their surface sterilization, were established on a modified MS nutrient medium (thiamine-HCl 1 mg L-1, nicotinic acid 1 mg L-1, pyridoxine-HCl 1 mg L-1) with 5 μΜ ΒΑ. Clean explants were transferred in the multiplication stage on a novel medium (Pear Medium 1), by adding 10 μΜ ΒΑ especially developed for Pyrus species. Due to poor culture development, the effect of photosynthetic photon flux density (PPFD) on the improvement of regeneration was studied. The exposure of explants to 10 μmol m-2 s-1 for the first two weeks followed by exposure to 35 μmol m-2 s-1 for another two weeks, was proved essential for the good development of cultures promoting both multiplication and elongation of explants. For further enhancement of shoot regeneration, the use of Pear Medium 1 with five different combinations of growth regulators was tested (BA, IBA). The most beneficial for the development of good quality shoots was 5 μΜ ΒΑ+0.0246 μΜ ΙΒΑ (4.67±0.40 new shoots per explant, elongation 1.28±0.13 cm). As multiplication was mainly based on axillary branching and the production of new shoots was still relatively low, the orientation of explants (horizontal vs upright position) in relation to the medium was investigated. Regeneration of shoots almost tripled, reaching 13.67 new shoots per explant in the case of horizontal orientation after the removal of the apical part (0.2 cm). The most successful rooting procedure (rooting: 83.33±5.89 %, root no: 6.20±0.49 roots per plantlet, root length: 0.56±0.05 cm) consisted of an initial stage of root induction maintaining microshoots in complete darkness for seven days. The rooting medium was a modified MS (1⁄2 NH4NO3, 1⁄2 KNO3) supplemented with 24.6 μΜ ΙΒΑ. Microshoots were subsequently transferred to a root development stage in the same rooting medium without auxin, exposed to 10 μmol m-2 s-1 for another four weeks. Successful acclimatization (87.5 %) was achieved after six weeks on perlite.
{"title":"Critical factors affecting an efficient micropropagation protocol for Pyrus spinosa Forskk.","authors":"P. Tsoulpha, S. Alexandri, M. Tsaktsira","doi":"10.37855/jah.2018.v20i03.33","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.33","url":null,"abstract":"Almond-leaved tree is one of the most commonly found native forest species in Greece, exhibiting valuable properties and thus suitable for multipurpose silviculture. Several critical factors were studied for the development of a successful micropropagation protocol of Pyrus spinosa juvenile trees. Newly immerged shoots of three-year-old plants, after their surface sterilization, were established on a modified MS nutrient medium (thiamine-HCl 1 mg L-1, nicotinic acid 1 mg L-1, pyridoxine-HCl 1 mg L-1) with 5 μΜ ΒΑ. Clean explants were transferred in the multiplication stage on a novel medium (Pear Medium 1), by adding 10 μΜ ΒΑ especially developed for Pyrus species. Due to poor culture development, the effect of photosynthetic photon flux density (PPFD) on the improvement of regeneration was studied. The exposure of explants to 10 μmol m-2 s-1 for the first two weeks followed by exposure to 35 μmol m-2 s-1 for another two weeks, was proved essential for the good development of cultures promoting both multiplication and elongation of explants. For further enhancement of shoot regeneration, the use of Pear Medium 1 with five different combinations of growth regulators was tested (BA, IBA). The most beneficial for the development of good quality shoots was 5 μΜ ΒΑ+0.0246 μΜ ΙΒΑ (4.67±0.40 new shoots per explant, elongation 1.28±0.13 cm). As multiplication was mainly based on axillary branching and the production of new shoots was still relatively low, the orientation of explants (horizontal vs upright position) in relation to the medium was investigated. Regeneration of shoots almost tripled, reaching 13.67 new shoots per explant in the case of horizontal orientation after the removal of the apical part (0.2 cm). The most successful rooting procedure (rooting: 83.33±5.89 %, root no: 6.20±0.49 roots per plantlet, root length: 0.56±0.05 cm) consisted of an initial stage of root induction maintaining microshoots in complete darkness for seven days. The rooting medium was a modified MS (1⁄2 NH4NO3, 1⁄2 KNO3) supplemented with 24.6 μΜ ΙΒΑ. Microshoots were subsequently transferred to a root development stage in the same rooting medium without auxin, exposed to 10 μmol m-2 s-1 for another four weeks. Successful acclimatization (87.5 %) was achieved after six weeks on perlite.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48913736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.37855/jah.2018.v20i03.40
Abera Tilahun, Begashaw Manahlie, G. Abebe, Genet Negash
This study was carried out to determine the effects of cutting position (tip, middle and basal) and different concentrations of IBA (IBA) (0, 5, 7.5 and 11g/L) on the rooting and shooting performance of Araucaria heterophylla. Two factorial experiments were conducted. Number of leaf, number of adventitious root and root length produced by cutting materials and the survival rates were measured. These parameters were significantly influenced by the interactive effect of cutting position and hormonal concentration. The interaction between tip cuttings and 11 g/L IBA concentration had higher root number, leaf number and root length as compared other interactions had not rooting and shooting performance on the cutting materials. Vegetative propagation of A. heterophylla through cuttings can be appropriately achieved by treating cuttings with IBA.
{"title":"The response of cutting position and auxin concentration on rooting and shooting of Araucaria heterophylla","authors":"Abera Tilahun, Begashaw Manahlie, G. Abebe, Genet Negash","doi":"10.37855/jah.2018.v20i03.40","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.40","url":null,"abstract":"This study was carried out to determine the effects of cutting position (tip, middle and basal) and different concentrations of IBA (IBA) (0, 5, 7.5 and 11g/L) on the rooting and shooting performance of Araucaria heterophylla. Two factorial experiments were conducted. Number of leaf, number of adventitious root and root length produced by cutting materials and the survival rates were measured. These parameters were significantly influenced by the interactive effect of cutting position and hormonal concentration. The interaction between tip cuttings and 11 g/L IBA concentration had higher root number, leaf number and root length as compared other interactions had not rooting and shooting performance on the cutting materials. Vegetative propagation of A. heterophylla through cuttings can be appropriately achieved by treating cuttings with IBA.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44134967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.37855/jah.2018.v20i03.31
A. Erst, A. Gorbunov, A. Karakulov
The effective methods of rooting and acclimatization in the sphagnum moss of in vitro propagated microshoots of commercially promising plants: Rhododendron ledebourii, R. hybridum cv. ‘Cunningham’s White’ and Vaccinium uliginosum cv. ‘Golubaya rossyp’ were developed for the first time. Two methods were studied: 1) rooting in vitro and acclimatization in the substrate and 2) rooting and acclimatization ex vitro in the substrate. Taking into account two factors (rooting of microshoots only in vitro conditions and only from already rooted in vitro regenerants), we have achieved 100 % of rooting of bog blueberry cv. ‘Golubaya rossyp’ and 73 % of plants adapted to ex vitro conditions. The method of ex vitro rooting in the substrate has been proved as effective for R. ledebourii, rooting of it’s shoots reached 80-90 % with 87 % viable plants. R. hybridum cv. ‘Cunningham’s White’ showed the greatest plasticity among the studied species and cultivars on ability to rooting of microshoots. All tested methods gave up to 90-100 % rooted and adapted plants of this cultivar. It was revealed that in vitro rooting microshoots, followed by acclimatization in the substrate was optimal for V. uliginosum cv. ‘Golubaya rossyp’; for R. ledebourii – the rooting and acclimatization in the substrate; and for R. hybridum cv. ‘Cunningham’s White’ both methods were effective. From a commercial point of view, the proposed methods decrease the costs of plant production significantly through a reduction in the time and labour needed to obtain well-rooted and acclimatized Ericaceae microplants. Therefore, it could make the micropropagation of commercially promising Ericacea plants in the nursery industry both possible and profitable.
{"title":"Rooting and acclimatization of in vitro propagated microshoots of the Ericaceae","authors":"A. Erst, A. Gorbunov, A. Karakulov","doi":"10.37855/jah.2018.v20i03.31","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.31","url":null,"abstract":"The effective methods of rooting and acclimatization in the sphagnum moss of in vitro propagated microshoots of commercially promising plants: Rhododendron ledebourii, R. hybridum cv. ‘Cunningham’s White’ and Vaccinium uliginosum cv. ‘Golubaya rossyp’ were developed for the first time. Two methods were studied: 1) rooting in vitro and acclimatization in the substrate and 2) rooting and acclimatization ex vitro in the substrate. Taking into account two factors (rooting of microshoots only in vitro conditions and only from already rooted in vitro regenerants), we have achieved 100 % of rooting of bog blueberry cv. ‘Golubaya rossyp’ and 73 % of plants adapted to ex vitro conditions. The method of ex vitro rooting in the substrate has been proved as effective for R. ledebourii, rooting of it’s shoots reached 80-90 % with 87 % viable plants. R. hybridum cv. ‘Cunningham’s White’ showed the greatest plasticity among the studied species and cultivars on ability to rooting of microshoots. All tested methods gave up to 90-100 % rooted and adapted plants of this cultivar. It was revealed that in vitro rooting microshoots, followed by acclimatization in the substrate was optimal for V. uliginosum cv. ‘Golubaya rossyp’; for R. ledebourii – the rooting and acclimatization in the substrate; and for R. hybridum cv. ‘Cunningham’s White’ both methods were effective. From a commercial point of view, the proposed methods decrease the costs of plant production significantly through a reduction in the time and labour needed to obtain well-rooted and acclimatized Ericaceae microplants. Therefore, it could make the micropropagation of commercially promising Ericacea plants in the nursery industry both possible and profitable.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49261964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.37855/jah.2018.v20i03.41
D. A. Animasaun, S. Oyedeji, R. Ingalhalli, R. Krishnamurthy
This study assessed the genetic diversity of six ornamental ferns in parks and gardens in Nigeria using inter-simple sequence repeat (ISSR) markers. Genomic DNA were extracted from the samples and Polymerase Chain Reaction (PCR) was performed using seven ISSR markers. The elcetrophoregram showed amplicon size ranged from 100bp-1Kb among the ferns. A total of 87 bands were generated with 71.26 % polymorphism and 28.73 % monomorphic bands. The average number of band per primer was 12.4 and polymorphism range was between 31.23-100 %, the highest polymorphism was obtained with ISSR2. The mean Nei’s genetic diversity was 29 % while the Shannon’s index was 43.5 %. The pair of Adiatum capilus-veneris and Pteris acanthoneura had maximum genetic distance of 0.6592 while A. capilus-veneris and A. furcans are the closest species. At genetic similarity of 78 %, the cluster analysis revealed two major groups. Group-1 comprised of four species, where P. acanthoneura is a distant member, the other three species in the group are closely related. Group-2 on the other hand had two closely related Adiantum species. The study concludes that ISSR markers are effective in the genetic study of the ferns and the genetic diversity information provided could be utilized for selection, improvement and conservation of the ornamental plants.
{"title":"Polymorphism and genetic diversity assessment of some ornamental ferns by microsatellite (ISSR) markers","authors":"D. A. Animasaun, S. Oyedeji, R. Ingalhalli, R. Krishnamurthy","doi":"10.37855/jah.2018.v20i03.41","DOIUrl":"https://doi.org/10.37855/jah.2018.v20i03.41","url":null,"abstract":"This study assessed the genetic diversity of six ornamental ferns in parks and gardens in Nigeria using inter-simple sequence repeat (ISSR) markers. Genomic DNA were extracted from the samples and Polymerase Chain Reaction (PCR) was performed using seven ISSR markers. The elcetrophoregram showed amplicon size ranged from 100bp-1Kb among the ferns. A total of 87 bands were generated with 71.26 % polymorphism and 28.73 % monomorphic bands. The average number of band per primer was 12.4 and polymorphism range was between 31.23-100 %, the highest polymorphism was obtained with ISSR2. The mean Nei’s genetic diversity was 29 % while the Shannon’s index was 43.5 %. The pair of Adiatum capilus-veneris and Pteris acanthoneura had maximum genetic distance of 0.6592 while A. capilus-veneris and A. furcans are the closest species. At genetic similarity of 78 %, the cluster analysis revealed two major groups. Group-1 comprised of four species, where P. acanthoneura is a distant member, the other three species in the group are closely related. Group-2 on the other hand had two closely related Adiantum species. The study concludes that ISSR markers are effective in the genetic study of the ferns and the genetic diversity information provided could be utilized for selection, improvement and conservation of the ornamental plants.","PeriodicalId":39205,"journal":{"name":"Journal of Applied Horticulture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46508039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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