Pub Date : 1993-09-30DOI: 10.14921/JSCC1971B.22.3_143
Hideaki Kinoshita, T. Ikeda, K. Takayama, T. Usui
{"title":"Serum Leucine Aminopeptidase Assay Using a Dialysis Membrane-Covered Glassy-Carbon Electrode","authors":"Hideaki Kinoshita, T. Ikeda, K. Takayama, T. Usui","doi":"10.14921/JSCC1971B.22.3_143","DOIUrl":"https://doi.org/10.14921/JSCC1971B.22.3_143","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1993-09-30DOI: 10.14921/JSCC1971B.22.3_147
F. Mashige, N. Takai, N. Shinozuka, H. Wada, I. Sakuma, A. Ito, Y. Matsushima, A. Ohkubo
{"title":"Development of a High-Performance Liquid Chromatography System with Multi-Electrode Electrochemical Detectors for Determination of Levels of Neurotransmitters in Cerebrospinal Fluid","authors":"F. Mashige, N. Takai, N. Shinozuka, H. Wada, I. Sakuma, A. Ito, Y. Matsushima, A. Ohkubo","doi":"10.14921/JSCC1971B.22.3_147","DOIUrl":"https://doi.org/10.14921/JSCC1971B.22.3_147","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1993-06-30DOI: 10.14921/JSCC1971B.22.2_75
H. Katsunuma, Y. Terano, A. Tomii, T. Shibata, Y. Sato
{"title":"Production, Characterization and Application of Antibodies against Elastase","authors":"H. Katsunuma, Y. Terano, A. Tomii, T. Shibata, Y. Sato","doi":"10.14921/JSCC1971B.22.2_75","DOIUrl":"https://doi.org/10.14921/JSCC1971B.22.2_75","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1993-06-30DOI: 10.14921/JSCC1971B.22.2_93
K. Ichihara, T. Ono, T. Kashiwai, Sachihiko Watanabe, Y. Endo, K. Miyai
{"title":"Purification and Chromatographic Property of Recombinant Human Thyroid-Stimulating Hormone","authors":"K. Ichihara, T. Ono, T. Kashiwai, Sachihiko Watanabe, Y. Endo, K. Miyai","doi":"10.14921/JSCC1971B.22.2_93","DOIUrl":"https://doi.org/10.14921/JSCC1971B.22.2_93","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1993-06-30DOI: 10.14921/JSCC1971B.22.2_99
S. Eda, A. Nakagawa, M. Kohno, Kimio Kurashima
In order to establish a speedy and convenient assay of serum CA 19-9, we developed a latex photometric immunoassay of the antigen. Latex reagent was prepared by physical binding of F(ab')2 fragment of murine monoclonal antibody (1116 NS 19-9) onto latex particles. Latex reagent showed dose-dependent agglutination after being mixed with CA 19-9 antigen. The latex assay required only 10 minutes and could be automated by using conventional clinical chemistry analyzer, COBAS MIRA. Intensity of signal of the reaction could be adjusted by modifying the concentration of reaction accelerating polymer, polyvinyl pyrrolidone K90 (PVP K90). When we used the assay buffer containing 2 g/l of PVP K90, minimum detection dosage was 2 units/ml and measuring range was up to 400 units/ml; they were similar to those of radioimmunoassav (RIA) and enzyme immunoassay (EIA). Within and between assay precisions were both about 5% in coefficient of variation (CVI at normal ranae and about 1-2% in CV at increased level. Correlation versus commercially available EIA was y(Latex assay)=1.08x(EIA)+ 3.41, r=0.981(n=101). Prozone phenomenon could be detected by utilizing mechanism of the instrument. These results indicate that this assay can be applied to daily analysis in clinical laboratories as a speedy and convenient substitute of RIA and .EIA.
{"title":"Development of Latex Photometric Immunoassay of Serum CA 19-9 Applicable to Conventional Clinical Chemistry Analyzers","authors":"S. Eda, A. Nakagawa, M. Kohno, Kimio Kurashima","doi":"10.14921/JSCC1971B.22.2_99","DOIUrl":"https://doi.org/10.14921/JSCC1971B.22.2_99","url":null,"abstract":"In order to establish a speedy and convenient assay of serum CA 19-9, we developed a latex photometric immunoassay of the antigen. Latex reagent was prepared by physical binding of F(ab')2 fragment of murine monoclonal antibody (1116 NS 19-9) onto latex particles. Latex reagent showed dose-dependent agglutination after being mixed with CA 19-9 antigen. The latex assay required only 10 minutes and could be automated by using conventional clinical chemistry analyzer, COBAS MIRA. Intensity of signal of the reaction could be adjusted by modifying the concentration of reaction accelerating polymer, polyvinyl pyrrolidone K90 (PVP K90). When we used the assay buffer containing 2 g/l of PVP K90, minimum detection dosage was 2 units/ml and measuring range was up to 400 units/ml; they were similar to those of radioimmunoassav (RIA) and enzyme immunoassay (EIA). Within and between assay precisions were both about 5% in coefficient of variation (CVI at normal ranae and about 1-2% in CV at increased level. Correlation versus commercially available EIA was y(Latex assay)=1.08x(EIA)+ 3.41, r=0.981(n=101). Prozone phenomenon could be detected by utilizing mechanism of the instrument. These results indicate that this assay can be applied to daily analysis in clinical laboratories as a speedy and convenient substitute of RIA and .EIA.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1993-03-31DOI: 10.14921/JSCC1971B.22.1_29
Y. Matsuda, R. Ishii, K. Miyazaki, K. Takeuchi, T. Hashimoto, S. Akihama
{"title":"Detection of Arginine Amidases Released from Isolated Rabbit Arteries","authors":"Y. Matsuda, R. Ishii, K. Miyazaki, K. Takeuchi, T. Hashimoto, S. Akihama","doi":"10.14921/JSCC1971B.22.1_29","DOIUrl":"https://doi.org/10.14921/JSCC1971B.22.1_29","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1992-12-31DOI: 10.14921/JSCC1971B.21.4_245
Y. Aoki, H. Ihara, Hiroomi Nakamura, T. Aoki, Mitsutaka Yoshida
{"title":"Rates of Oxidation of Bilirubin Species by Peroxidase With or Without Serum Albumin","authors":"Y. Aoki, H. Ihara, Hiroomi Nakamura, T. Aoki, Mitsutaka Yoshida","doi":"10.14921/JSCC1971B.21.4_245","DOIUrl":"https://doi.org/10.14921/JSCC1971B.21.4_245","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1992-12-31DOI: 10.14921/JSCC1971B.21.4_249
Y. Tazuke, N. Kodama, K. Adachi, Y. Tsukada, R. Uenoyama, F. Takeda, Kazufumi Suzuki, S. Baba
{"title":"A New Enzymatic Assay Method of Sulfated Bile Acids in Urine","authors":"Y. Tazuke, N. Kodama, K. Adachi, Y. Tsukada, R. Uenoyama, F. Takeda, Kazufumi Suzuki, S. Baba","doi":"10.14921/JSCC1971B.21.4_249","DOIUrl":"https://doi.org/10.14921/JSCC1971B.21.4_249","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1992-09-30DOI: 10.14921/JSCC1971B.21.3_159
M. Miyagawa, M. Oyama, Y. Ikeda
{"title":"Determination of Anti-Bovine Serum Albumin (BSA) and Anti-Ovalbumin (OA) Antibodies in Healthy Human Sera with Solid Phase Enzyme Immunoassay (SP-EIA) Based on the End Point Method","authors":"M. Miyagawa, M. Oyama, Y. Ikeda","doi":"10.14921/JSCC1971B.21.3_159","DOIUrl":"https://doi.org/10.14921/JSCC1971B.21.3_159","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1992-09-30DOI: 10.14921/JSCC1971B.21.3_216
H. Ihara, Hiroomi Nakamura, Tai‐Wing Wu, Y. Aoki, T. Aoki, Mitsutaka Yoshida
{"title":"Unexpected Presence of Delta Bilirubin in the Serum of a Patient with Type I Crigler-Najjar Syndrome Observed by a Kodak Ektachem 700N Analyzer","authors":"H. Ihara, Hiroomi Nakamura, Tai‐Wing Wu, Y. Aoki, T. Aoki, Mitsutaka Yoshida","doi":"10.14921/JSCC1971B.21.3_216","DOIUrl":"https://doi.org/10.14921/JSCC1971B.21.3_216","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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