Pub Date : 1992-06-30DOI: 10.14921/JSCC1971B.21.2_127
Hideaki Kinoshita, T. Usui, Etsuko Oishi, T. Kakiuchi, T. Ikeda
{"title":"Serum Alkaline Phosphatase Assay Using a Dialysis Membrane-Covered Glassy-Carbon Electrode","authors":"Hideaki Kinoshita, T. Usui, Etsuko Oishi, T. Kakiuchi, T. Ikeda","doi":"10.14921/JSCC1971B.21.2_127","DOIUrl":"https://doi.org/10.14921/JSCC1971B.21.2_127","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1992-03-31DOI: 10.14921/JSCC1971B.21.1_6
S. Saheki, Y. Hitsumoto, O. Doi, M. Murase, N. Takeuchi
{"title":"Inhibitory Effects of Aprotinin on Apoprotein Degradation by Polymorphonuclear Leucocytes and Elastase in vitro in Serum Amyloid A Protein-Rich High Density Lipoprotein","authors":"S. Saheki, Y. Hitsumoto, O. Doi, M. Murase, N. Takeuchi","doi":"10.14921/JSCC1971B.21.1_6","DOIUrl":"https://doi.org/10.14921/JSCC1971B.21.1_6","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1992-03-31DOI: 10.14921/JSCC1971B.21.1_1
Y. Aoki, H. Ihara, Hiroomi Nakamura, T. Aoki, Mitsutaka Yoshida
{"title":"Delta Bilirubin: Its Isolation by Precipitation with Ammonium Sulfate and Properties","authors":"Y. Aoki, H. Ihara, Hiroomi Nakamura, T. Aoki, Mitsutaka Yoshida","doi":"10.14921/JSCC1971B.21.1_1","DOIUrl":"https://doi.org/10.14921/JSCC1971B.21.1_1","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.14921/JSCC1971B.21.1_1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1992-03-31DOI: 10.14921/JSCC1971B.21.1_13
K. Mawatari, F. Iinuma, Mitsuo Watanabe
A high-performance liquid chromatographic method involving a postcolumn photochemical reaction and fluorometric detection has been developed for the determination of nalidixic acid concentration in human urine. The mobile phase consists of 0.1 M potassium dihydrogenphosphate-sodium tetraborate buffer (pH 8.6)-methanol (75 : 25, v/v) containing 20 mM hydrogen peroxide. The compound in the column effluent is irradiated with ultraviolet light to produce fluorescence. This fluorescence is monitored with excitation at 380 nm and emission at 440 nm. The calibration curve for nalidixic acid is linear over the range of 0.2-300 ng/injection. The mean recovery from urine is nearly 100%.
{"title":"Fluorometric Determination of Nalidixic Acid in Human Urine Using High-Performance Liquid Chromatography with Postcolumn Photochemical Reaction","authors":"K. Mawatari, F. Iinuma, Mitsuo Watanabe","doi":"10.14921/JSCC1971B.21.1_13","DOIUrl":"https://doi.org/10.14921/JSCC1971B.21.1_13","url":null,"abstract":"A high-performance liquid chromatographic method involving a postcolumn photochemical reaction and fluorometric detection has been developed for the determination of nalidixic acid concentration in human urine. The mobile phase consists of 0.1 M potassium dihydrogenphosphate-sodium tetraborate buffer (pH 8.6)-methanol (75 : 25, v/v) containing 20 mM hydrogen peroxide. The compound in the column effluent is irradiated with ultraviolet light to produce fluorescence. This fluorescence is monitored with excitation at 380 nm and emission at 440 nm. The calibration curve for nalidixic acid is linear over the range of 0.2-300 ng/injection. The mean recovery from urine is nearly 100%.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-12-31DOI: 10.14921/JSCC1971B.20.4_235
T. Shiraishi, H. Kondo, H. Tsubota
A new method for measuring the activity of serum pyruvate kinase (PK:EC 2.7.1.40) was developed by using a thermostable glucokinase (Glck) from a thermophilic bacterium, Bacillus stearothermophilus. The ATP formed by the reaction of PK is finally converted to NADPH via glucose-6-phosphate by the action of Glck and glucose-6-phosphate dehydrogenase. The change in absorbance at 340nm was found to be linear up to about 5000U/I of PK. The within-run and day-to-day coefficients of variationwere 1.13% at 85.4U/I and 2.08% at 45.7U/I, respectively. The influence of various coexistents and anticoagulants, such as bilirubin, ascorbate, glucose, EDTA, sodium fluoride, etc., on the assay was negligible. The reagent was stable in solution for about one month at 10°C. A method for simultaneously measuring the activities of PK and creatine kinase (CK) in a single specimen was also developed. This was based on the fact that the assay conditions for both enzymes were similar. This method was found to have a high degree of precision and a good correlation with respective PK and CK assay methods. This simultaneous measurement may be useful for the accurate differential diagnosis of myocardial infarction.
{"title":"A New Method for Measuring Serum Pyruvate Kinase and Creatine Kinase Activities Using a Thermostable Glucokinase","authors":"T. Shiraishi, H. Kondo, H. Tsubota","doi":"10.14921/JSCC1971B.20.4_235","DOIUrl":"https://doi.org/10.14921/JSCC1971B.20.4_235","url":null,"abstract":"A new method for measuring the activity of serum pyruvate kinase (PK:EC 2.7.1.40) was developed by using a thermostable glucokinase (Glck) from a thermophilic bacterium, Bacillus stearothermophilus. The ATP formed by the reaction of PK is finally converted to NADPH via glucose-6-phosphate by the action of Glck and glucose-6-phosphate dehydrogenase. The change in absorbance at 340nm was found to be linear up to about 5000U/I of PK. The within-run and day-to-day coefficients of variationwere 1.13% at 85.4U/I and 2.08% at 45.7U/I, respectively. The influence of various coexistents and anticoagulants, such as bilirubin, ascorbate, glucose, EDTA, sodium fluoride, etc., on the assay was negligible. The reagent was stable in solution for about one month at 10°C. A method for simultaneously measuring the activities of PK and creatine kinase (CK) in a single specimen was also developed. This was based on the fact that the assay conditions for both enzymes were similar. This method was found to have a high degree of precision and a good correlation with respective PK and CK assay methods. This simultaneous measurement may be useful for the accurate differential diagnosis of myocardial infarction.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-12-31DOI: 10.14921/JSCC1971B.20.4_242
T. Niwa, T. Koshiyama, S. Ishiwata, J. Goto, T. Nambara
{"title":"High-Performance Liquid Chromatography of Estrogen 3-Sulfates with Electrochemical Detection Using an Immobilized Enzyme Reactor","authors":"T. Niwa, T. Koshiyama, S. Ishiwata, J. Goto, T. Nambara","doi":"10.14921/JSCC1971B.20.4_242","DOIUrl":"https://doi.org/10.14921/JSCC1971B.20.4_242","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66735014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-12-31DOI: 10.14921/JSCC1971B.20.4_203
H. Akabane, T. Takatori, K. Terazawa, M. Nagao
{"title":"Leukotoxin Synthesis and Its Effects on Blood Pressure of Guinea Pigs","authors":"H. Akabane, T. Takatori, K. Terazawa, M. Nagao","doi":"10.14921/JSCC1971B.20.4_203","DOIUrl":"https://doi.org/10.14921/JSCC1971B.20.4_203","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.14921/JSCC1971B.20.4_203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-09-30DOI: 10.14921/JSCC1971B.20.3_153
Kunio Kobayashi, N. Ogasahara, A. Matsuoka, T. Takumi, Hajime Nakamura
{"title":"Glycated Lipoproteins in Serum from Insulin-Dependent (IDDM) and Non-Insulin-Dependent (NIDDM) Diabetic Patients","authors":"Kunio Kobayashi, N. Ogasahara, A. Matsuoka, T. Takumi, Hajime Nakamura","doi":"10.14921/JSCC1971B.20.3_153","DOIUrl":"https://doi.org/10.14921/JSCC1971B.20.3_153","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-09-30DOI: 10.14921/JSCC1971B.20.3_117
Y. Azuma, T. Kanno
{"title":"The Changes of Cytosolic Aminopeptidase Activities in Mitogen-Stimulated T and B Lymphocyte","authors":"Y. Azuma, T. Kanno","doi":"10.14921/JSCC1971B.20.3_117","DOIUrl":"https://doi.org/10.14921/JSCC1971B.20.3_117","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-09-30DOI: 10.14921/JSCC1971B.20.3_156
Hideo Takahashi, T. Iida, S. Nakagawa
{"title":"Flow-Cytometric Studies of the Response of Neutrophils and/or Cultured Myocardial Cells to Anoxia and Reoxygenation","authors":"Hideo Takahashi, T. Iida, S. Nakagawa","doi":"10.14921/JSCC1971B.20.3_156","DOIUrl":"https://doi.org/10.14921/JSCC1971B.20.3_156","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1991-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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