Pub Date : 1989-06-30DOI: 10.14921/JSCC1971B.18.1_14
T. Ohara, M. Sakai, N. Igaki, S. Masuta, T. Nakamichi, F. Hata, M. Oimomi, S. Baba
In order to estimate the fructosamine level in plasma we measure fructoselysine, which consists of glucose bound to a lysine residue of serum proteins. Thus, it is possible that the fructosamine level can be affected by the level of serum proteins. Therefore, an in vitro study was performed to elucidete the relationship between fructosamine level and the concentrations of albumin and gamma-globulin. The incubation of albumin and gamma-globulin with varying concentrations of glucose showed that the levels of fructosamine increased in proportion to the concentrations of albumin and gamma-globulin. At given concentrations of albumin and gamma-globulin, the level of fructosamine increased with the concentration of glucose. The glycation of gammaglobulin was found in much lower concentration than of albumin. It was suggested that influence of glycated gamma-globulin can be ignored during fructosamine assay.
{"title":"Glycation of Albumin and Gamma-Globulin in Vitro","authors":"T. Ohara, M. Sakai, N. Igaki, S. Masuta, T. Nakamichi, F. Hata, M. Oimomi, S. Baba","doi":"10.14921/JSCC1971B.18.1_14","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.1_14","url":null,"abstract":"In order to estimate the fructosamine level in plasma we measure fructoselysine, which consists of glucose bound to a lysine residue of serum proteins. Thus, it is possible that the fructosamine level can be affected by the level of serum proteins. Therefore, an in vitro study was performed to elucidete the relationship between fructosamine level and the concentrations of albumin and gamma-globulin. The incubation of albumin and gamma-globulin with varying concentrations of glucose showed that the levels of fructosamine increased in proportion to the concentrations of albumin and gamma-globulin. At given concentrations of albumin and gamma-globulin, the level of fructosamine increased with the concentration of glucose. The glycation of gammaglobulin was found in much lower concentration than of albumin. It was suggested that influence of glycated gamma-globulin can be ignored during fructosamine assay.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-06-30DOI: 10.14921/JSCC1971B.18.1_29
M. Oimomi, T. Nakamichi, M. Sakai, T. Ohara, N. Igaki, F. Hata, S. Baba
{"title":"Role of Fructose in Glycation","authors":"M. Oimomi, T. Nakamichi, M. Sakai, T. Ohara, N. Igaki, F. Hata, S. Baba","doi":"10.14921/JSCC1971B.18.1_29","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.1_29","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-06-30DOI: 10.14921/JSCC1971B.18.1_7
S. Saheki, A. Takeda, K. Shishino, M. Murase, N. Takeuchi
{"title":"Changes in High Density Lipoproteins with High Content of Serum Amyloid A Protein by the Incubation with Polymorphonuclear Leucocytes","authors":"S. Saheki, A. Takeda, K. Shishino, M. Murase, N. Takeuchi","doi":"10.14921/JSCC1971B.18.1_7","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.1_7","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-12-30DOI: 10.14921/JSCC1971B.17.3_136
K. Kanaya, K. Hiromi, M. Totani, T. Murachi
{"title":"Rapid Assay of Lactate Dehydrogenase in Serum bythe Stopped-Flow Method","authors":"K. Kanaya, K. Hiromi, M. Totani, T. Murachi","doi":"10.14921/JSCC1971B.17.3_136","DOIUrl":"https://doi.org/10.14921/JSCC1971B.17.3_136","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-12-30DOI: 10.14921/JSCC1971B.17.3_125
M. Oimomi, N. Igaki, T. Ohara, M. Sakai, T. Nakamichi, F. Hata, Y. Maeda, S. Baba
{"title":"Increased Levels of Fluorescent Products of the Maillard Reaction Generated by 3-Deoxyglucosone","authors":"M. Oimomi, N. Igaki, T. Ohara, M. Sakai, T. Nakamichi, F. Hata, Y. Maeda, S. Baba","doi":"10.14921/JSCC1971B.17.3_125","DOIUrl":"https://doi.org/10.14921/JSCC1971B.17.3_125","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-12-30DOI: 10.14921/JSCC1971B.17.3_111
S. Saheki, A. Takeda, K. Tokunaga, M. Murase, N. Takeuchi
{"title":"High Density Lipoproteins in the Patients with High Serum Amyloid A Proteins","authors":"S. Saheki, A. Takeda, K. Tokunaga, M. Murase, N. Takeuchi","doi":"10.14921/JSCC1971B.17.3_111","DOIUrl":"https://doi.org/10.14921/JSCC1971B.17.3_111","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-12-30DOI: 10.14921/JSCC1971B.17.3_121
M. Oimomi, N. Igaki, S. Masuta, F. Hata, Y. Maeda, S. Nishimoto, Y. Kitamura, S. Matsumoto, S. Baba
{"title":"Correlation between the Accumulation of Advanced-Stage Maillard Product in Cataractous Lens Protein and Diabetic Neuropathy","authors":"M. Oimomi, N. Igaki, S. Masuta, F. Hata, Y. Maeda, S. Nishimoto, Y. Kitamura, S. Matsumoto, S. Baba","doi":"10.14921/JSCC1971B.17.3_121","DOIUrl":"https://doi.org/10.14921/JSCC1971B.17.3_121","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-12-30DOI: 10.14921/JSCC1971B.17.3_107
M. Oimomi, S. Masuta, N. Igaki, T. Nakamichi, Y. Maeda, F. Hata, Y. Kitamura, S. Nishimoto, S. Matsumoto, S. Baba
{"title":"Clinical Application of Serum Fructosamine Measurement as an Indicator of Blood Glucose Control","authors":"M. Oimomi, S. Masuta, N. Igaki, T. Nakamichi, Y. Maeda, F. Hata, Y. Kitamura, S. Nishimoto, S. Matsumoto, S. Baba","doi":"10.14921/JSCC1971B.17.3_107","DOIUrl":"https://doi.org/10.14921/JSCC1971B.17.3_107","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-10-31DOI: 10.14921/JSCC1971B.17.2_91
M. Oimomi, F. Hata, N. Igaki, S. Masuta, T. Nakamichi, Y. Maeda, S. Nishimoto, S. Matsumoto, S. Baba
{"title":"Enzyme Inhibition of the Advanced-Stage of the Maillard Reaction","authors":"M. Oimomi, F. Hata, N. Igaki, S. Masuta, T. Nakamichi, Y. Maeda, S. Nishimoto, S. Matsumoto, S. Baba","doi":"10.14921/JSCC1971B.17.2_91","DOIUrl":"https://doi.org/10.14921/JSCC1971B.17.2_91","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-10-31DOI: 10.14921/JSCC1971B.17.2_51
K. Nakashima, Miyuki Muraoka, S. Nakatsuji, S. Akiyama
A sensitive and simple high-performance liquid chromatographic determination method for D-penicillamine in serum was developed. The method is based on the fluorescence derivazation of D-penicillamine with N-[4-(6-dimethylamino-2-benzofuranyl)phenyl]maleimide and following fluorescence detection by high-performance liquid chromatography . A calibration graph for D-penicillamine was linear over the range from 200 fmol to 12 pmol , and the lower limit of detection at a signal-to-noise ratio of 2 was 50 fmol. Recovery rate of 2.9 ƒÊM of D-penicillamine was 91.8% (C.V.=4.2%, n=5). Time courses of serum levels of D-penicillamine after single oral administration of D-penicilamine in capsule to three women were examined. Serum levels reached maximum after 2 hr and then decreased gradually till 24 hr. This method will be useful for a monitoring of D-penicillamine in blood.
{"title":"Determination of D-Penicillamine in Serum by High-Performance Liquid Chromatography Using N -[4-(6-Dimethylamino-2-benzofuranyl) phenyl] maleimide","authors":"K. Nakashima, Miyuki Muraoka, S. Nakatsuji, S. Akiyama","doi":"10.14921/JSCC1971B.17.2_51","DOIUrl":"https://doi.org/10.14921/JSCC1971B.17.2_51","url":null,"abstract":"A sensitive and simple high-performance liquid chromatographic determination method for D-penicillamine in serum was developed. The method is based on the fluorescence derivazation of D-penicillamine with N-[4-(6-dimethylamino-2-benzofuranyl)phenyl]maleimide and following fluorescence detection by high-performance liquid chromatography . A calibration graph for D-penicillamine was linear over the range from 200 fmol to 12 pmol , and the lower limit of detection at a signal-to-noise ratio of 2 was 50 fmol. Recovery rate of 2.9 ƒÊM of D-penicillamine was 91.8% (C.V.=4.2%, n=5). Time courses of serum levels of D-penicillamine after single oral administration of D-penicilamine in capsule to three women were examined. Serum levels reached maximum after 2 hr and then decreased gradually till 24 hr. This method will be useful for a monitoring of D-penicillamine in blood.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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