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Production of Highly Purified Imidazole Dipeptides from Animal Extracts and Development of Foods to Prevent Lifestyle-Related Diseases 从动物提取物中生产高纯度咪唑二肽及预防生活方式相关疾病的食品开发
Q4 Engineering Pub Date : 2020-09-15 DOI: 10.11301/JSFE.20578
Kenichiro Sato, Shigenobu Shiotani, N. Yanai
Imidazole dipeptides are believed to have the ability to alleviate fatigue and improve cognitive function. We developed a production procedure to isolate and purify imidazole dipeptides from animal extracts. The highly purified imidazole dipeptides obtained by this method are almost tasteless, odorless, and free of impurities such as sodium and creatinine, making them functional food ingredients. We conducted a clinical study using the highly purified imidazole dipeptides and found that ingestion of a drink containing three antioxidants: 400 mg imidazole dipeptides, 300 mg ascorbic acid, and 20 mg ferulic acid for 8 weeks improved the oxidative injury of peripheral blood lymphocyte DNA in middle-aged and elderly men. Furthermore, elderly persons with mild cognitive impairment who were given imidazole dipeptides at a daily dose of 1 g for 12 weeks showed improved scores on both the global clinical dementia rating and the mini-mental state test. From these findings, it was suggested that highly purified imidazole dipeptides may be a potential food ingredient for preventing lifestyle-related diseases.
咪唑二肽被认为具有减轻疲劳和改善认知功能的能力。我们开发了一种从动物提取物中分离和纯化咪唑二肽的生产工艺。通过这种方法获得的高度纯化的咪唑二肽几乎无味、无臭,不含钠和肌酸酐等杂质,使其成为功能性食品成分。我们使用高度纯化的咪唑二肽进行了一项临床研究,发现连续8周摄入含有三种抗氧化剂的饮料:400mg咪唑二肽、300mg抗坏血酸和20mg阿魏酸,可以改善中老年男性外周血淋巴细胞DNA的氧化损伤。此外,患有轻度认知障碍的老年人,每天服用1g咪唑二肽12周,在全球临床痴呆评级和迷你精神状态测试中的得分都有所提高。这些发现表明,高度纯化的咪唑二肽可能是预防生活方式相关疾病的潜在食品成分。
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引用次数: 0
Determining the Internal Structure of Ice Cream Using Cryogenic Microtome Imaging and X-ray Computed Tomography 利用低温显微成像和X射线计算机断层扫描技术测定冰淇淋内部结构
Q4 Engineering Pub Date : 2020-09-15 DOI: 10.11301/JSFE.20568
G. Do, S. Sase, R. Kobayashi, Masugu Sato, Yeonghwan Bae, Tatsuro Maeda, S. Ueno, T. Araki
College of Bioresource Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan 2 Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan College of Life Science and Natural Resources, Sunchon National University, 255 Jungangro, Suncheon, Jeollanamdo 57922, Korea Faculty of Health and Medical Science, Teikyo Heisei University, 2-51-4 Higashi-Ikebukuro, Toshima, Tokyo 170-8445, Japan Faculty of Education, Saitama University, 255 Shimo Okubo, Sakura, Saitama 338-8570, Japan Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan
日本大学生物资源科学学院,1866 Kameino,Fujisawa,神奈川252-0880,日本2日本同步辐射研究所,1-1-1 Kouto,Sayo,兵库679-5198,日本顺川国立大学生命科学与自然资源学院,255 Jungangro,Suncheon,Jeollanamdo 57922,Teikyo平成大学韩国健康与医学院,2-51-4东池袋,Toshima,Tokyo 170-8445,日本教育学院,埼玉大学,255 Shimo Okubo,Sakura,Saitama 338-8570,日本农业和生命科学研究生院,东京大学,1-1-1 Yayoi,Bunkyo,Tokyo113-8657
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引用次数: 1
Study on the Stabilization Technique by Encapsulation of Biomolecules Such Food Proteins 食品蛋白等生物分子的包封稳定技术研究
Q4 Engineering Pub Date : 2020-09-15 DOI: 10.11301/JSFE.20579
K. Imamura
One of the methodologies for avoiding the deterioration of labile food and biological substances during drying is to embed the labiles in amorphous matrix of sugar. In a series of the authors ’ studies, various sugars were first compared for the stabilizing effect on proteins in freeze-drying as well as the physicochemical properties in the amorphous state. Based on the experimental results, three generally accepted hypotheses on the protein stabilization by amorphous sugar matrix (water substitution hypothesis; glassy state hypothesis; partition effect) were merged into the compromised one. Next, in order to further improve the protein stabilization technique, the remaining enzyme activities after freeze-drying in the presence of various classes of substances were analyzed. The combination of disaccharide with polysaccharide(s), certain types of surfactants ( e.g. , sugar esters), and proteinaceous substances were found to exhibit markedly high protein stabilizing effect. Furthermore, the amorphous-sugar-based matrix was applied to homogeneously encapsulating oil droplets and nanoparticles during freeze-drying, which successfully avoided the aggregation of the particulates and the dropout from the resulting dried objects. The application of amorphous sugar matrix was expanded to the solid dispersion of hydrophobic flavors at molecular level, by utilizing the finding on the over-saturation of sugars in an organic solvent.
避免不稳定食品和生物物质在干燥过程中变质的方法之一是将不稳定物包埋在无定形的糖基质中。在作者的一系列研究中,首先比较了各种糖在冷冻干燥过程中对蛋白质的稳定作用以及无定形状态下的物理化学性质。基于实验结果,提出了非晶态糖基质稳定蛋白质的三种普遍接受的假说(水取代假说;玻态假说;分割效应)被合并成折衷的。接下来,为了进一步改进蛋白质稳定技术,我们分析了在不同种类物质存在下冷冻干燥后的剩余酶活性。研究发现,双糖与多糖、某些类型的表面活性剂(如糖酯)和蛋白质物质的结合具有显著的高蛋白质稳定作用。此外,在冷冻干燥过程中,将非晶糖基基质应用于油滴和纳米颗粒的均匀包裹,成功地避免了颗粒的聚集和干燥后物体的脱落。利用糖在有机溶剂中的过饱和现象,将无定形糖基质的应用扩展到疏水香精在分子水平上的固体分散。
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引用次数: 0
Discoloration Kinetics of Carthamus Red in Aqueous Ethanol 红花红在乙醇水溶液中的褪色动力学
Q4 Engineering Pub Date : 2020-09-15 DOI: 10.11301/JSFE.20576
S. Adachi, Y. Miyagawa
The solubility of carthamus red, red pigment from safflower, in aqueous ethanol largely depended on the ethanol concentration, and it was the highest near 60 and 70% (v/v) ethanol. Discoloration of carthamus red in aqueous ethanol was monitored under temperature-programmed heating conditions where the temperature of test solution raised linearly with time to evaluate the activation energy and frequency factor. The activation energy and frequency factor did not depend on the ethanol concentration, suggesting that ethanol does not affect the discoloration mechanism. The activation energy was 78±5 kJ/mol. The increase in absorbance at 390 nm during discoloration of carthamus red correlated with the decrease in absorbance at 520 nm. This fact also suggests no effect of ethanol on discoloration mechanism of carthamus red.
红花红色素红花红在乙醇水溶液中的溶解度在很大程度上取决于乙醇浓度,在60%和70%(v/v)乙醇附近溶解度最高。在程序升温条件下监测红花红在乙醇水溶液中的变色,其中测试溶液的温度随时间线性升高,以评估活化能和频率因子。活化能和频率因子不依赖于乙醇浓度,表明乙醇不影响变色机制。活化能为78±5kJ/mol。红花红变色过程中在390nm处吸光度的增加与在520nm处吸光度下降相关。这一事实也表明乙醇对红花红的变色机理没有影响。
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引用次数: 0
Characteristic of Co-Culture Biofilm Formed by Lactobacillus plantarum and Pediococcus acidilactici, and Antagonistic Effects of This Biofilm on Pathogen Growth 植物乳杆菌与乳酸球菌共培养生物膜的特性及其对病原菌生长的拮抗作用
Q4 Engineering Pub Date : 2020-06-15 DOI: 10.11301/jsfe.20564
T. Nguyen, Hop Van Duong, T. Nguyen, Mai Thi Ngoc Dinh, M. H. Nguyen
Recent studies in lactic acid bacteria (LAB) biofilm formation have reported that the functional properties of LAB are associated with biofilm formation. In this study, Pediococcus acidilactici and Lactobacillus plantarum showed the ability to form a biofilm on a solid surface under static conditions. The morphology of LAB on MRS-CR agar showed that both P. acidilactici and L. plantarum could produce exopolysaccharide (EPS) on the cell surface which mainly contributed to forming the biofilm o f single and co-culture strains on solid surfaces. Under the flow condition, cell motility played an important role in supporting the cell to overcome the repulsive force to reach and interact with the surface. As a result, the L. plantarum and co-culture cells with higher motility caused a noticeably thicker biofilm on the glass surface compared to the P. acidilactici strain. Moreover, the antagonistic activity assay showed that co-culture biofilm formation improved the antagonistic effects of LAB against Pseudomonas aeruginosa and Salmonella typhimurium compared to those in planktonic culture. It suggested that co-culture biofilm could be an effective model to apply useful LAB as potential probiotics and to control pathogen in food chain, especially under the flow condition.
最近对乳酸菌(LAB)生物膜形成的研究表明,LAB的功能特性与生物膜的形成有关。在这项研究中,乳酸球菌和植物乳杆菌显示出在静态条件下在固体表面形成生物膜的能力。乳酸杆菌和植物乳杆菌均能在细胞表面产生胞外多糖(EPS),这主要有助于单个和共培养菌株在固体表面形成生物膜。在流动条件下,细胞运动在支持细胞克服排斥力到达表面并与表面相互作用方面发挥着重要作用。因此,与乳酸乳杆菌菌株相比,植物乳杆菌和具有更高运动性的共培养细胞在玻璃表面上引起了明显更厚的生物膜。此外,拮抗活性测定表明,与浮游培养相比,共培养生物膜的形成提高了LAB对铜绿假单胞菌和鼠伤寒沙门氏菌的拮抗作用。这表明,共培养生物膜可能是一种有效的模式,可以应用有用的LAB作为潜在的益生菌,并控制食物链中的病原体,特别是在流动条件下。
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引用次数: 3
Effect of Near Infrared Irradiation on Quality of Fresh-cut Lettuce During Storage 近红外辐射对鲜切生菜贮藏品质的影响
Q4 Engineering Pub Date : 2020-06-15 DOI: 10.11301/jsfe.19561
Kota Kobayashi, Y. Takiguchi, M. Takashio, Toshinori Takei, Kakibuchi Kazumasa, Ayako Hada, Y. Ishida, Y. Ogawa
The effect of near-infrared (NIR) irradiation before storage on the quality of fresh-cut lettuce was investigated in this study. It was shown that the NIR irradiation was effective in suppressing the number of viable bacteria and weight loss during the storage of cut lettuce, statistically. However, it was also shown that the browning ratio increased slightly under the stronger NIR irradiation conditions of the 30 s and 60 s, which could deteriorate the appearance quality. This was thought to be the result of increase in the Phenylalanine Ammonia Lyase (PAL) activity and total polyphenol content. Meanwhile, no increase in browning ratio was observed at 10 s, and there was no difference in PAL activity and total polyphenol content from the control. In the range of irradiation conditions set in this study, thus, the 10 s NIR irradiation was expected to be effective in suppressing the number of general viable bacteria and mass,
研究了贮藏前近红外辐射对鲜切生菜品质的影响。结果表明,近红外辐射在抑制生菜贮藏过程中的活菌数量和失重方面是有效的。然而,在30s和60s的较强近红外辐射条件下,褐变率略有增加,这可能会降低外观质量。这被认为是苯丙氨酸解氨酶(PAL)活性和总多酚含量增加的结果。同时,在10s时,褐变率没有增加,PAL活性和总多酚含量与对照组没有差异。因此,在本研究中设定的照射条件范围内,预计10s近红外照射可有效抑制一般活菌的数量和质量,
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引用次数: 0
Changes in the Volatile Profiles of Commercial Straight Pressed Tomato Juice Upon Heating 商品直榨番茄汁加热后挥发特性的变化
Q4 Engineering Pub Date : 2020-06-15 DOI: 10.11301/jsfe.20570
Yumi Iwasaki, Y. Otagiri, A. Obata, Yoko Iijima
Tomato juice is a popular beverage and a convenient cooking ingredient. Especially, straight pressed tomato juice (not from concentrate) maintains fresh flavor, showing different characteristics from the juice from concentrate. We studied changes in the profile of volatile compounds in straight pressed tomato juice upon heating. We heated two types of commercial packaged straight pressed tomato juices at 95 100°C for 0 5 h and extracted volatile compounds in each sample by headspacesolid phase microextraction. A total of 176 and 187 compound peaks were detected, of which 81 and 86 compounds were identified or tentatively identified by GC/MS analysis, respectively. Principal component analysis (PCA) indicated that the samples could be divided into six groups based on heating time; the volatile profiles changed in a time-dependent manner. Furthermore, seven chemicals were identified as heat markers using par tial least squares (PLS) regression, namely 2-pentylfuran, phenylacetaldehyde, hexanal, fur fural, cyclohexanone, β-damascenone, and 1,4-dimethylbenzene. A prediction model based on only these seven marker profiles for heating period, also showed good linear regression (R>0.987). Gas chromatography-mass spectrometry/olfactometry (GC-MS/O) analysis and quantification of these volatiles suggested that phenylacetaldehyde, hexanal, β-damascenone and 2-pentylfuran influence the flavor characteristics of tomato juice.
番茄汁是一种受欢迎的饮料,也是一种方便的烹饪原料。特别是直榨番茄汁(非浓缩)保持了鲜嫩的风味,表现出与浓缩番茄汁不同的特点。我们研究了直榨番茄汁中挥发性化合物在加热后的变化。将两种商品包装直榨番茄汁在95 100℃下加热0.5 h,采用顶空固相微萃取法提取样品中的挥发性化合物。共检测到176个和187个化合物峰,其中经GC/MS鉴定或初步鉴定的化合物分别为81个和86个。主成分分析(PCA)表明,根据加热时间可将样品分为6组;易失性曲线随时间变化。此外,使用偏最小二乘法(PLS)回归鉴定了7种化学物质作为热标记物,即2-戊基呋喃、苯乙醛、己醛、糠醛、环己酮、β-大马士革酮和1,4-二甲苯。仅基于这7个标记剖面的升温期预测模型也具有良好的线性回归(R = 0.9987)。气相色谱-质谱/嗅觉(GC-MS/O)分析和定量表明,苯乙醛、己醛、β-马马酮和2-戊基呋喃影响番茄汁的风味特性。
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引用次数: 0
Acoustic Methods for Characterizing Physical Properties of Food Products 表征食品物理特性的声学方法
Q4 Engineering Pub Date : 2020-06-15 DOI: 10.11301/jsfe.20571
T. Nishizu
We found that the change with time of milk curd strength during the process of lactic acid fermentation and the change with time of freezing rate during the freezing process of food material can be monitored by measuring ultrasonic velocity. While the ultrasonic velocities of fruits and vegetables depend on not the “hardness” but the gas volume fractions of their tissues, the propagation velocities of impact vibration have strong correlations with their elastic properties. We have developed a measurement system for volume/density of food material by using phenomena of Helmholtz acoustic resonance. We found that the Helmholtz resonant frequency for food material having a pore structure is lower than that of non-porous food material having the same volume. Based on the theoretical consideration on the relations between the physical properties of porous food and the reduction of resonant frequency, we have also developed a measurement system for evaluating beer foam characteristics and bread texture. In another study we have found that the frequency of crack vibrations generated during chewing deep-fried foods has a close correlation with the results of sensory evaluation of their crispness.
我们发现,通过测量超声波速度,可以监测乳酸发酵过程中乳凝乳强度随时间的变化以及食品材料冷冻过程中冷冻速率随时间的改变。虽然水果和蔬菜的超声波速度不取决于其组织的“硬度”,而是取决于其气体体积分数,但冲击振动的传播速度与其弹性特性有很强的相关性。我们利用亥姆霍兹声共振现象开发了一种食品材料体积/密度的测量系统。我们发现,具有孔结构的食品材料的亥姆霍兹共振频率低于具有相同体积的无孔食品材料的共振频率。基于对多孔食品物理特性与共振频率降低之间关系的理论考虑,我们还开发了一个评估啤酒泡沫特性和面包质地的测量系统。在另一项研究中,我们发现咀嚼油炸食品时产生的裂纹振动频率与对其脆度的感官评估结果密切相关。
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引用次数: 0
Drying and Rehydration Kinetics of Noodles 面条的干燥和再水化动力学
Q4 Engineering Pub Date : 2020-03-15 DOI: 10.11301/jsfe.20567
Takenobu Ogawa
Drying is the oldest method for preserving food, and rehydration is one of the simplest methods of cooking. The behavior of water transfer inside foods during the drying and rehydration processes varies depending on the manufacturing and cooking conditions, respectively, and the food quality is strongly affected by the behavior. Existing manufacturing and cooking processes, which have relied on great experience and build through trial and error, cannot quickly and accurately respond to the diverse demands of recent years, such as health consciousness and insatiable pursuit for palatability. Therefore, the theoretical study that enables rational food production has been strongly demanded. However, the phenomena and mechanisms of water transfer and their effects on the quality are not well understood. Focusing on the pasta with relatively simple composition, we have elucidated the phenomenon and its mechanism of water transfer occurring during drying and rehydration processes, and also investigated the effect of the water transfer on pasta quality. This review provides the overview of our findings.
干燥是保存食物最古老的方法,而补液是最简单的烹饪方法之一。在干燥和再水化过程中,食物内部的水转移行为分别取决于制造和烹饪条件,并且这种行为对食品质量有很大影响。现有的制造和烹饪工艺依赖于丰富的经验,并通过反复试验建立起来,无法快速准确地响应近年来的各种需求,例如健康意识和对美味的永不满足的追求。因此,迫切需要进行合理粮食生产的理论研究。然而,人们对水转移的现象和机制及其对水质的影响尚不清楚。本文以成分相对简单的面食为研究对象,阐述了面食干燥和复水过程中水分传递的现象及其机理,并探讨了水分传递对面食品质的影响。这篇综述概述了我们的研究结果。
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引用次数: 0
Enhanced Efficiency of Carotenoid Processing Utilizing Change in Physicochemical Properties Induced by Z-isomerization 利用Z异构化引起的理化性质变化提高类胡萝卜素加工效率
Q4 Engineering Pub Date : 2020-03-15 DOI: 10.11301/jsfe.20565
M. Honda
Carotenoids, the most common fat-soluble plant pigments in nature, have attracted considerable attention because of their powerful antioxidant activity and potential to prevent of various diseases, such as cancer an d arteriosclerosis. These compounds have numerous geometric isomers owing to E / Z -isomerization at arbitrary sites of the multiple conjugated double bonds. However, in nature, most carotenoids are present in the all- E -configuration. (all- E )-Carotenoids are characterized by low solubility in solvents and high crystallinity. Thus, these characteristics result in the decreased efficiency of carotenoid processing, such as extraction and micronization. On the other hand, Z -isomerization of carotenoids induces change in physicochemical properties. For example, the solubility of carotenoids is markedly improved and they change from a “ crystalline state ” to an “ oily (amorphous) state ” . Utilizing these changes in physicochemical properties of carotenoids, we successfully improved the efficiency of carotenoid processing. The objective of this paper is to review the effect of Z -isomerization of carotenoids on the physicochemical properties and its application to carotenoid processing, such as extraction, emulsification, and micronization. Furthermore, aspects of Z -isomerization methods of carotenoids and effects of Z -isomerization of carotenoids on the bioavailability and bioactivity are also included in this review.
类胡萝卜素是自然界中最常见的脂溶性植物色素,由于其强大的抗氧化活性和预防各种疾病的潜力,如癌症和动脉硬化,引起了人们的广泛关注。这些化合物由于在多个共轭双键的任意位置上的E/Z异构化而具有许多几何异构体。然而,在自然界中,大多数类胡萝卜素都以全E构型存在。(all-E)-类胡萝卜素的特点是在溶剂中溶解度低,结晶度高。因此,这些特性导致类胡萝卜素加工效率降低,例如提取和微粉化。另一方面,类胡萝卜素的Z异构化引起理化性质的变化。例如,类胡萝卜素的溶解度显著提高,它们从“结晶状态”变为“油性(无定形)状态”。利用类胡萝卜素理化性质的这些变化,我们成功地提高了类胡萝卜素加工的效率。本文综述了类胡萝卜素Z异构化对其理化性质的影响及其在类胡萝卜素加工中的应用,如提取、乳化和微粉化。此外,还对类胡萝卜素的Z异构化方法以及类胡萝卜素Z异构化对生物利用度和生物活性的影响进行了综述。
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引用次数: 2
期刊
Japan Journal of Food Engineering
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