Pub Date : 2013-12-13DOI: 10.3724/SP.J.1145.2013.00043
Cai Shaoli, Zou Youtu, Hu Jianzhong, Lin Lin
In order to improve the thermostability of Penicillium expansum lipase(PEL),the lipase gene was mutated by sitedirected mutagenesis.A recombinant plasmid pAO815-ep8-K115R which contains double mutant genes was constructed by overlap extension PCR using the cDNA of a random-mutant lipase ep8(a single site mutant) as the template and two special primers were used to generate another mutation site K115R.The recombinant vector was transformed into Pichia pastoris GS115 by electroporation and the recombinant mutant GS-pAO815-ep8-K115R can secret the double-mutant lipase PEL-ep8K115R-GS into the medium when it was induced by methanol.Thermostability analysis revealed that the residual activity of the double-mutant lipase PEL-ep8-K115R-GS after incubated at 40 ℃ for 30 min was 54% and 27% higher than that of the wild type lipase PEL-GS and the random-mutant lipase PEL-ep8-GS respectively.Tm of the double-mutant lipase PEL-ep8-K115RGS was 42.2 ℃,3.5 ℃ higher than that of the wild type lipase PEL-GS,and 2.0 ℃ higher than that of the random-mutant lipase PEL-ep8-GS.
{"title":"Improvement of Thermostability of Penicillium expansum Lipase by Site-directed Mutagenesis","authors":"Cai Shaoli, Zou Youtu, Hu Jianzhong, Lin Lin","doi":"10.3724/SP.J.1145.2013.00043","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00043","url":null,"abstract":"In order to improve the thermostability of Penicillium expansum lipase(PEL),the lipase gene was mutated by sitedirected mutagenesis.A recombinant plasmid pAO815-ep8-K115R which contains double mutant genes was constructed by overlap extension PCR using the cDNA of a random-mutant lipase ep8(a single site mutant) as the template and two special primers were used to generate another mutation site K115R.The recombinant vector was transformed into Pichia pastoris GS115 by electroporation and the recombinant mutant GS-pAO815-ep8-K115R can secret the double-mutant lipase PEL-ep8K115R-GS into the medium when it was induced by methanol.Thermostability analysis revealed that the residual activity of the double-mutant lipase PEL-ep8-K115R-GS after incubated at 40 ℃ for 30 min was 54% and 27% higher than that of the wild type lipase PEL-GS and the random-mutant lipase PEL-ep8-GS respectively.Tm of the double-mutant lipase PEL-ep8-K115RGS was 42.2 ℃,3.5 ℃ higher than that of the wild type lipase PEL-GS,and 2.0 ℃ higher than that of the random-mutant lipase PEL-ep8-GS.","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"43-47"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-13DOI: 10.3724/SP.J.1145.2013.00061
Xiang Zhang, Lei Ma, Yongqiang Tian, Guolin Zhang, Yinggang Luo
{"title":"Molecular Cloning and Heterologous Expression of Putative ( S )-norcoclaurine Synthases from Arabidopsis thaliana : Molecular Cloning and Heterologous Expression of Putative ( S )-norcoclaurine Synthases from Arabidopsis thaliana","authors":"Xiang Zhang, Lei Ma, Yongqiang Tian, Guolin Zhang, Yinggang Luo","doi":"10.3724/SP.J.1145.2013.00061","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00061","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"61-68"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69808005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-13DOI: 10.3724/SP.J.1145.2013.00054
F. Long, Yanling Jin, Yun Zhao, Xiaofeng Gao, Yuhao Li, Qian Chen, Hai Zhao
{"title":"Optimization of Butanol Production from Canna edulis Ker by Clostridium acetobutylicum Using Response Surface Methodology: Optimization of Butanol Production from Canna edulis Ker by Clostridium acetobutylicum Using Response Surface Methodology","authors":"F. Long, Yanling Jin, Yun Zhao, Xiaofeng Gao, Yuhao Li, Qian Chen, Hai Zhao","doi":"10.3724/SP.J.1145.2013.00054","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00054","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69808000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-13DOI: 10.3724/SP.J.1145.2013.00750
Li Ming, G. Jia, Shi Zheng-guo, Y. Libin, Yan Hong
The northeast of Qinghai-Tibetan Plateau is located in the convergence zone of the monsoon region of eastern China, northwest arid area and Qinghai-Tibetan Plateau region. During April to May 2012, an investigation was carried out on the bacterial community structure in ten lakes on northeastern Qinghai-Tibetan Plateau. With 16S rRNA sequencing and PCR-DGGE technology, twelve bacteria genus level libraries were constructed with a total of 117 operational taxonomic unit including 27 Betaproteobacteria, 8 Gamaproteobacteria, 21 Alphaproteobacteria, 7 Deltaproteobacteria,16 Acidobacteria, 2Deinococci, 5 Sphingobacteria, 6 Flavobacteria, 5 Nitrospira, 11 Actinobacteria, 6 Gemmatimonadetes and 3 unclassified bacteria. Most sequences in these studied lakes were closer to aquatic bacteria than any other bacterial clusters. The number of operational taxonomic unit in northeastern Qinghai-Tibetan Plateau was 32.1 ± 11.2, Shannon-Wiener diversity index 0.97± 0.29, Pielou's evenness index 0.19 ± 0.04, and Sorenson similarity index between lakes 0.31 ± 0.11, all comparatively low.Notably, the environment factors played an important role in bacteria community, with temperature positively related to OTUs(R = 0.763, P 0.05), to Shannon-Wiener diversity index(R = 0.708, P 0.05), and to Pielou's evenness index(R = 0.641, P 0.05); the lake area was also positively related to OTUs(R= 0.754, P 0.05) and Shannon-Wiener diversity index(R = 0.668, P 0.05). Water chemistry also played an important part in bacteria community. The result is helpful for study of climatic factors of community structure and sustainable development in this region.
青藏高原东北部处于中国东部季风区、西北干旱区和青藏高原地区的交汇地带。2012年4 ~ 5月，对青藏高原东北部10个湖泊的细菌群落结构进行了调查。通过16S rRNA测序和PCR-DGGE技术，构建了12个细菌属水平文库，共有117个操作分类单元，包括27个Betaproteobacteria、8个Gamaproteobacteria、21个Alphaproteobacteria、7个Deltaproteobacteria、16个Acidobacteria、2个deinococci、5个Sphingobacteria、6个Flavobacteria、5个Nitrospira、11个放线菌ia、6个gemmatimonadees和3个未分类细菌。在这些被研究的湖泊中，大多数序列比任何其他细菌簇更接近水生细菌。青藏高原东北部运行分类单元数为32.1±11.2,Shannon-Wiener多样性指数为0.97±0.29,Pielou均匀度指数为0.19±0.04,Sorenson相似性指数为0.31±0.11，均较低。环境因子对细菌群落的影响显著，温度与OTUs(R = 0.763, P 0.05)、Shannon-Wiener多样性指数(R = 0.708, P 0.05)、Pielou均匀度指数(R = 0.641, P 0.05)呈正相关;湖泊面积与OTUs(R= 0.754, P 0.05)和Shannon-Wiener多样性指数(R= 0.668, P 0.05)呈正相关。水化学在细菌群落中也起着重要作用。研究结果对该区群落结构和可持续发展的气候因子研究具有一定的指导意义。
{"title":"Bacterial Community Structure in Lakes on the Northeastern Qinghai-Tibetan Plateau","authors":"Li Ming, G. Jia, Shi Zheng-guo, Y. Libin, Yan Hong","doi":"10.3724/SP.J.1145.2013.00750","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00750","url":null,"abstract":"The northeast of Qinghai-Tibetan Plateau is located in the convergence zone of the monsoon region of eastern China, northwest arid area and Qinghai-Tibetan Plateau region. During April to May 2012, an investigation was carried out on the bacterial community structure in ten lakes on northeastern Qinghai-Tibetan Plateau. With 16S rRNA sequencing and PCR-DGGE technology, twelve bacteria genus level libraries were constructed with a total of 117 operational taxonomic unit including 27 Betaproteobacteria, 8 Gamaproteobacteria, 21 Alphaproteobacteria, 7 Deltaproteobacteria,16 Acidobacteria, 2Deinococci, 5 Sphingobacteria, 6 Flavobacteria, 5 Nitrospira, 11 Actinobacteria, 6 Gemmatimonadetes and 3 unclassified bacteria. Most sequences in these studied lakes were closer to aquatic bacteria than any other bacterial clusters. The number of operational taxonomic unit in northeastern Qinghai-Tibetan Plateau was 32.1 ± 11.2, Shannon-Wiener diversity index 0.97± 0.29, Pielou's evenness index 0.19 ± 0.04, and Sorenson similarity index between lakes 0.31 ± 0.11, all comparatively low.Notably, the environment factors played an important role in bacteria community, with temperature positively related to OTUs(R = 0.763, P 0.05), to Shannon-Wiener diversity index(R = 0.708, P 0.05), and to Pielou's evenness index(R = 0.641, P 0.05); the lake area was also positively related to OTUs(R= 0.754, P 0.05) and Shannon-Wiener diversity index(R = 0.668, P 0.05). Water chemistry also played an important part in bacteria community. The result is helpful for study of climatic factors of community structure and sustainable development in this region.","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"750-758"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69812311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-13DOI: 10.3724/SP.J.1145.2013.00037
P. Gai, Chuan-Gen Tang, Jingyuan Liu, Yan Liu, Chao Zhang, Z. Wu
{"title":"Asymmetric anti -Prelog Reduction of 3,5-bis(trifluoromethyl)-acetophenone by Microbacterium oxydans C3: Asymmetric anti -Prelog Reduction of 3,5-bis(trifluoromethyl)-acetophenone by Microbacterium oxydans C3","authors":"P. Gai, Chuan-Gen Tang, Jingyuan Liu, Yan Liu, Chao Zhang, Z. Wu","doi":"10.3724/SP.J.1145.2013.00037","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00037","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"37-42"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-13DOI: 10.3724/SP.J.1145.2013.00141
F. Shu, L. Kong, Saidi Wang, Fangfang Zi
{"title":"Chironomid Larva Community and Indication to Eutrophication Progress in Lake Nansi, Shandong, China: Chironomid Larva Community and Indication to Eutrophication Progress in Lake Nansi, Shandong, China","authors":"F. Shu, L. Kong, Saidi Wang, Fangfang Zi","doi":"10.3724/SP.J.1145.2013.00141","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00141","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"141-146"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69808084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cloning and Diversity Analysis of the Conserved Fragments of Pheromone Receptor Genes in Genus Agrocybe: Cloning and Diversity Analysis of the Conserved Fragments of Pheromone Receptor Genes in Genus Agrocybe","authors":"Yingying He, Weimin Chen, Yongchang Zhao, Dequn Zhou","doi":"10.3724/SP.J.1145.2013.00794","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00794","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"794-799"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69812732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-13DOI: 10.3724/SP.J.1145.2013.00833
J. Yong, S. Min, Z. Yao, Tao Yong, Li Daping
Reduction of CO2 to organics with bioelectrical systems has become a research area of environmental microbiology.This study constructed a bioelectrochemical system(BES) capable of reducing CO2 to CH4 and CH3COOH via abiotical H2 gas production and/or direct extracellular electron transfer. CH4 and CH3COOH were produced at rates of 17.3 mL h-1L-1 and 13.9 mg h-1L-1, respectively(at potential of-1 100 mV). The current capture efficiency reached 94.0% in batch potentiostatic experiments. Microbial characterization revealed that the microbial populations consisted of Methanobacterium palustre,Methanocorpusculum parvum, and acetogen Acetobacterium sp. These results suggested that mixed culture in BESs can convert CO2 into various organic compounds by accepting electrons in different ways.
利用生物电系统将CO2还原为有机物已成为环境微生物学的一个研究领域。本研究构建了一个生物电化学系统(BES)，该系统能够通过非生物产氢和/或直接胞外电子转移将CO2还原为CH4和CH3COOH。CH4和CH3COOH的产率分别为17.3 mL h-1L-1和13.9 mg h-1L-1(电位-1 100 mV)。在批量恒电位实验中，电流捕获效率达到94.0%。微生物鉴定结果表明，微生物群主要由palustre Methanobacterium,Methanocorpusculum parvum和Acetobacterium sp.组成。这些结果表明，BESs中混合培养可以通过不同的方式接受电子，将CO2转化为多种有机化合物。
{"title":"Simultaneous Production of Methane and Acetate from Carbon Dioxide with Bioelectrochemical Systems","authors":"J. Yong, S. Min, Z. Yao, Tao Yong, Li Daping","doi":"10.3724/SP.J.1145.2013.00833","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00833","url":null,"abstract":"Reduction of CO2 to organics with bioelectrical systems has become a research area of environmental microbiology.This study constructed a bioelectrochemical system(BES) capable of reducing CO2 to CH4 and CH3COOH via abiotical H2 gas production and/or direct extracellular electron transfer. CH4 and CH3COOH were produced at rates of 17.3 mL h-1L-1 and 13.9 mg h-1L-1, respectively(at potential of-1 100 mV). The current capture efficiency reached 94.0% in batch potentiostatic experiments. Microbial characterization revealed that the microbial populations consisted of Methanobacterium palustre,Methanocorpusculum parvum, and acetogen Acetobacterium sp. These results suggested that mixed culture in BESs can convert CO2 into various organic compounds by accepting electrons in different ways.","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"833-837"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69812748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-13DOI: 10.3724/SP.J.1145.2013.00781
Jian Li, Yi Liu, Cheng-zhen Wu, T. Hong, Yongming Lin, Han Lin, Can Chen, W. Hong
In order to investigate the resistance of Casuarina equisetifolia L. to autotoxicity and to provide scientific basis for continuous planting of C. equisetifolia, clones of ‘Huian No.1’ C. equisetifolia were water cultivated to study the effects of chlorophyll fluorescence in branchlet under allelochemical stress (of seven concentrations: 0, 12.5, 25, 50, 100, 200 and 400 mg L) induced by quercetin-3-α-araboside (allelochemical A) and quercetin-3-β-glucoside (allelochemical B). The membership function of fuzzy mathematics was used to analyze the tolerance abilities of C. equisetifolia seedling under different allelochemicals stress. The results showed slight variations in F0, Fm, Fv/F0 and Fv/Fm of the branchlet under slight allelochemicals stress (12.5, 25, 50 mg L). The Fv/Fm was observed to maintain 78% of normal photosynthesis reaction. The F0, qN, Drate, LPAR of the branchlet significantly (P < 0.05) increased with the concentration of allelochemicals(100, 200, 400 mg L), while the values of Fm, Fv/Fm, Fv/F0, Prate, ΦPSII, qP decreased significantly (P < 0.05). Allelochemicals stress damaged the function of PSII reaction center and decreased the photochemical electron transfer rate, which led to the structural damage of photosynthetic organs and functional degradation in photosynthetic ability. The range of chlorophyll fluorescence parameters in branchlet of C. equisetifolia varied with different allelochemicals,the seedlings had better stress tolerance against allelochemical A than allelochemical B. Fig 3, Tab 1, Ref 28
{"title":"Effect of Two Allelochemicals of Casuarina equisetifolia L. on Chlorophyll Fluorescence Parameters in Hydroponically Cultured Seedling: Effect of Two Allelochemicals of Casuarina equisetifolia L. on Chlorophyll Fluorescence Parameters in Hydroponically Cultured Seedling","authors":"Jian Li, Yi Liu, Cheng-zhen Wu, T. Hong, Yongming Lin, Han Lin, Can Chen, W. Hong","doi":"10.3724/SP.J.1145.2013.00781","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00781","url":null,"abstract":"In order to investigate the resistance of Casuarina equisetifolia L. to autotoxicity and to provide scientific basis for continuous planting of C. equisetifolia, clones of ‘Huian No.1’ C. equisetifolia were water cultivated to study the effects of chlorophyll fluorescence in branchlet under allelochemical stress (of seven concentrations: 0, 12.5, 25, 50, 100, 200 and 400 mg L) induced by quercetin-3-α-araboside (allelochemical A) and quercetin-3-β-glucoside (allelochemical B). The membership function of fuzzy mathematics was used to analyze the tolerance abilities of C. equisetifolia seedling under different allelochemicals stress. The results showed slight variations in F0, Fm, Fv/F0 and Fv/Fm of the branchlet under slight allelochemicals stress (12.5, 25, 50 mg L). The Fv/Fm was observed to maintain 78% of normal photosynthesis reaction. The F0, qN, Drate, LPAR of the branchlet significantly (P < 0.05) increased with the concentration of allelochemicals(100, 200, 400 mg L), while the values of Fm, Fv/Fm, Fv/F0, Prate, ΦPSII, qP decreased significantly (P < 0.05). Allelochemicals stress damaged the function of PSII reaction center and decreased the photochemical electron transfer rate, which led to the structural damage of photosynthetic organs and functional degradation in photosynthetic ability. The range of chlorophyll fluorescence parameters in branchlet of C. equisetifolia varied with different allelochemicals,the seedlings had better stress tolerance against allelochemical A than allelochemical B. Fig 3, Tab 1, Ref 28","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"781-786"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69812655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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