Pub Date : 2013-12-13DOI: 10.3724/SP.J.1145.2013.00800
Liu Yang, Zhonghua Wei, Fei Zhongan, Zhao Feng, Qiao Dai-rong, C. Yi
Housefly(Musca domestica) is an important mechanical and biological vector harboring and transmitting human and animal pathogens. To study the composition of bacterial community in housefly midgut and assess the role of housefly in the epidemiology of diseases, 16S rRNA gene clone library of bacteria in housefly midgut was constructed. The library compromised of 88 sequences, which were grouped into 22 OTUs, including Proteobacteria(12 OTUs), Firmicutes(9 OTUs), and Bacteroidetes(1 OTU). Twelve OTUs of Proteobacteria belonged to Betaproteobacteria(1 OTU), Epsinoproteobacteria(1 OTU)and Gammaproteobacteria(10 OTUs) respectively. No Alphaproteobacteria or Deltaproteobacteria was found. The 9 Firmicutes included Lactobacillales(6 OTUs), Clostridia(1 OTU) and Bacillales(2 OTUs). In the library, Gammaproteobacteria was the dominant phylotype, which compromised 71 sequences(80.7%). Some species in Enterobacter, Klebsiella, Providencia, Proteus,Aeromonas, and Staphylococcus in the library were pathogens or opportunistic pathogens to human and animal. These results help to understand the significant roles of housefly in disease transmitting.
{"title":"Composition of Bacterial Community in Musca domestica Midgut","authors":"Liu Yang, Zhonghua Wei, Fei Zhongan, Zhao Feng, Qiao Dai-rong, C. Yi","doi":"10.3724/SP.J.1145.2013.00800","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00800","url":null,"abstract":"Housefly(Musca domestica) is an important mechanical and biological vector harboring and transmitting human and animal pathogens. To study the composition of bacterial community in housefly midgut and assess the role of housefly in the epidemiology of diseases, 16S rRNA gene clone library of bacteria in housefly midgut was constructed. The library compromised of 88 sequences, which were grouped into 22 OTUs, including Proteobacteria(12 OTUs), Firmicutes(9 OTUs), and Bacteroidetes(1 OTU). Twelve OTUs of Proteobacteria belonged to Betaproteobacteria(1 OTU), Epsinoproteobacteria(1 OTU)and Gammaproteobacteria(10 OTUs) respectively. No Alphaproteobacteria or Deltaproteobacteria was found. The 9 Firmicutes included Lactobacillales(6 OTUs), Clostridia(1 OTU) and Bacillales(2 OTUs). In the library, Gammaproteobacteria was the dominant phylotype, which compromised 71 sequences(80.7%). Some species in Enterobacter, Klebsiella, Providencia, Proteus,Aeromonas, and Staphylococcus in the library were pathogens or opportunistic pathogens to human and animal. These results help to understand the significant roles of housefly in disease transmitting.","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"800-804"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69812824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-13DOI: 10.3724/SP.J.1145.2013.00164
Jing Xiong, Xiao-Yun Ren, L. Ding, Jing Xie, X. Liao
{"title":"Ligand Fishing from Rheum palmatum Extract Using Human Serum Albumin Functionalized Magnetic Nanoparticles: Ligand Fishing from Rheum palmatum Extract Using Human Serum Albumin Functionalized Magnetic Nanoparticles","authors":"Jing Xiong, Xiao-Yun Ren, L. Ding, Jing Xie, X. Liao","doi":"10.3724/SP.J.1145.2013.00164","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00164","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"164-167"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69808424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soil microbial process plays an important role in the conversion of soil organic carbon into inorganic carbon,which is significant for the global carbon cycle.The aim of this study was to investigate the microbial functional diversity of carbon source utilization in three vegetation types under natural conditions along Sangong River basin in Fukang,Xinjiang,China.The three typical vegetation types were dominated by Haloxylon ammodendron,Reaumuria soongorica,and Cleistogenes chinensis,respectively.The soil type is saline soil in this area with varied alkalinity.BIOLOG method was used to determine capability of soil microorganisms in utilizing 31 types of single carbon source.Correlation between soil physicochemical property(pH,water content,total soluble salt) and AWCD(the average well color development),Shannon diversity index,richness value were analyzed.Microbial AWCD in H.ammodendron plot was significantly higher than that of the other two plots(P0.01).pH value(pearson correlation=0.798,P0.01) and total carbon content(pearson correlation=0.655,P0.05) of the soil were closely associated with the value of AWCD,while the effect of water content and total soluble salt was not significant.In this study,the H.ammodendron vegetation had the highest soil total carbon content as well as the highest soil alkalinity while the ability of utilizing carbon source of its soil microbial community was higher than the other two vegetation types.The analysis of normalised optical density(OD) data with PCA(principal component analysis) showed that eleven carbon sources(five types of carbohydrates,two types of amino acid,four types of carboxylic acids) were well used by the soil microbes of H.ammodendron vegetation,six carbon sources(one of the carbohydrates,three of amino acid,two of carboxylic acids) were better metabolized in R.soongorica community.In addition,the soil microbes in C.chinensis vegetation only effectively utilized five out of thirty-one single carbon sources.It was assumed that higher total carbon content and alkalinity would result in higher utilization in the soil microbial community.This result will be benefit for the management of desertification and will contribute to the understanding of the underground carbon process in the saline soil in arid area and the research works on carbon cycle.
{"title":"Carbon Source Utilization of Microbes in Saline Soil of Three Vegetation Types in Xinjiang, China: Carbon Source Utilization of Microbes in Saline Soil of Three Vegetation Types in Xinjiang, China","authors":"Qian Luo, Baoling Huang, Zhi-xi Tang, L. Lai, Wei Wei, Yuanrun Zheng","doi":"10.3724/SP.J.1145.2013.00096","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00096","url":null,"abstract":"Soil microbial process plays an important role in the conversion of soil organic carbon into inorganic carbon,which is significant for the global carbon cycle.The aim of this study was to investigate the microbial functional diversity of carbon source utilization in three vegetation types under natural conditions along Sangong River basin in Fukang,Xinjiang,China.The three typical vegetation types were dominated by Haloxylon ammodendron,Reaumuria soongorica,and Cleistogenes chinensis,respectively.The soil type is saline soil in this area with varied alkalinity.BIOLOG method was used to determine capability of soil microorganisms in utilizing 31 types of single carbon source.Correlation between soil physicochemical property(pH,water content,total soluble salt) and AWCD(the average well color development),Shannon diversity index,richness value were analyzed.Microbial AWCD in H.ammodendron plot was significantly higher than that of the other two plots(P0.01).pH value(pearson correlation=0.798,P0.01) and total carbon content(pearson correlation=0.655,P0.05) of the soil were closely associated with the value of AWCD,while the effect of water content and total soluble salt was not significant.In this study,the H.ammodendron vegetation had the highest soil total carbon content as well as the highest soil alkalinity while the ability of utilizing carbon source of its soil microbial community was higher than the other two vegetation types.The analysis of normalised optical density(OD) data with PCA(principal component analysis) showed that eleven carbon sources(five types of carbohydrates,two types of amino acid,four types of carboxylic acids) were well used by the soil microbes of H.ammodendron vegetation,six carbon sources(one of the carbohydrates,three of amino acid,two of carboxylic acids) were better metabolized in R.soongorica community.In addition,the soil microbes in C.chinensis vegetation only effectively utilized five out of thirty-one single carbon sources.It was assumed that higher total carbon content and alkalinity would result in higher utilization in the soil microbial community.This result will be benefit for the management of desertification and will contribute to the understanding of the underground carbon process in the saline soil in arid area and the research works on carbon cycle.","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"96-104"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69808017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-13DOI: 10.3724/SP.J.1145.2013.00838
Haiwei Ren, Jinping Li, Y. Zhang, Yonggang Wang, Xiaoqian Chen, Zhizhong Li
{"title":"Optimization of Saccharification for Distiller’s Grains and Ethanol Fermentation: Optimization of Saccharification for Distiller’s Grains and Ethanol Fermentation","authors":"Haiwei Ren, Jinping Li, Y. Zhang, Yonggang Wang, Xiaoqian Chen, Zhizhong Li","doi":"10.3724/SP.J.1145.2013.00838","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00838","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"838-844"},"PeriodicalIF":0.0,"publicationDate":"2013-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69812803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00643
Z. Tan, Q. Fu, G. Peng, Hongjuan Yuan, Yanbo Cheng, Yuan Jiang
In order to investigate the diversity of endophytic diazotrophs in Cymbopogon caesius and Miscanthu floridulus, thirty and thirty-six isolates were obtained respectively from these two forage grasses by using two selective medium without nitrogen sources in combination with acetylene reduction assay. The sixty-six endophytic diazotrophs were assigned to 10 groups (61 strains, at least 3 for each group from I to X) and five ungrouped strains by analysis of SDS-PAGE (dodecyl sulfate sodium salt polyacrylamide gel electrophoresis) patterns of whole-cell protein electrophoresis and IS-PCR (Insertion sequence-based PCR) DNA fingerprinting. 16S rDNA sequencing analysis of representative strains of each group showed that these strains were closely related to Burkholderia cepacia, Enterobacter cloacae, Phytobacter diazotrophicus, Enterobacter asburiae, Herbaspirillum seropedicae, Cronobacter sakazakii, Enterobacter oryzae, Klebsiella pneumoniae, Enterobacter dissolvens, Azospirillum amazonense, and Cronobacter dublinensis, indicating the high diversity of endophytic diazotrophs colonized in the host plants of C. caesius and M. floridulus. The results of physiological and biochemical tests showed that some isolates had the ability of ammonia secreting, indole producing, and phosphorus and potassium dissolving, suggesting the potential application in agricultural practices. Fig 2, Tab 3, Ref 38
{"title":"Identification and Characterization of Endophytic Diazotrophs Isolated from Cymbopogon caesius and Miscanthus floridulus: Identification and Characterization of Endophytic Diazotrophs Isolated from Cymbopogon caesius and Miscanthus floridulus","authors":"Z. Tan, Q. Fu, G. Peng, Hongjuan Yuan, Yanbo Cheng, Yuan Jiang","doi":"10.3724/SP.J.1145.2013.00643","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00643","url":null,"abstract":"In order to investigate the diversity of endophytic diazotrophs in Cymbopogon caesius and Miscanthu floridulus, thirty and thirty-six isolates were obtained respectively from these two forage grasses by using two selective medium without nitrogen sources in combination with acetylene reduction assay. The sixty-six endophytic diazotrophs were assigned to 10 groups (61 strains, at least 3 for each group from I to X) and five ungrouped strains by analysis of SDS-PAGE (dodecyl sulfate sodium salt polyacrylamide gel electrophoresis) patterns of whole-cell protein electrophoresis and IS-PCR (Insertion sequence-based PCR) DNA fingerprinting. 16S rDNA sequencing analysis of representative strains of each group showed that these strains were closely related to Burkholderia cepacia, Enterobacter cloacae, Phytobacter diazotrophicus, Enterobacter asburiae, Herbaspirillum seropedicae, Cronobacter sakazakii, Enterobacter oryzae, Klebsiella pneumoniae, Enterobacter dissolvens, Azospirillum amazonense, and Cronobacter dublinensis, indicating the high diversity of endophytic diazotrophs colonized in the host plants of C. caesius and M. floridulus. The results of physiological and biochemical tests showed that some isolates had the ability of ammonia secreting, indole producing, and phosphorus and potassium dissolving, suggesting the potential application in agricultural practices. Fig 2, Tab 3, Ref 38","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"643-649"},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69811637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00727
Wei Kang, Yue Sun, Lufeng Sun, Zhao-li Xu, Jianglong Que
{"title":"Distribution of Phytoplankton in Radial Sand Ridge Area in North Jiangsu Shoal: Distribution of Phytoplankton in Radial Sand Ridge Area in North Jiangsu Shoal","authors":"Wei Kang, Yue Sun, Lufeng Sun, Zhao-li Xu, Jianglong Que","doi":"10.3724/SP.J.1145.2013.00727","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00727","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"48 1","pages":"727-733"},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69812618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00587
Lin Yongming, Wei-Dong Yu, Yi Liu, Shujun Yan, W. Hong, Can Chen, Jian Li, Cheng-zhen Wu
{"title":"Characteristics of Branching Pattern and Crown Morphology of Casuarina equisetifolia L. in Different Coastal Distances: Characteristics of Branching Pattern and Crown Morphology of Casuarina equisetifolia L. in Different Coastal Distances","authors":"Lin Yongming, Wei-Dong Yu, Yi Liu, Shujun Yan, W. Hong, Can Chen, Jian Li, Cheng-zhen Wu","doi":"10.3724/SP.J.1145.2013.00587","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00587","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"23 1","pages":"587-592"},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69810747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-12DOI: 10.3724/SP.J.1145.2013.00704
C. Ke, Bisha Wu, Qin Shao, Wei Yang, Jianzhong Huang
{"title":"Construction of Saccharomyces cescerevisiae Mutant with Overexpression of PDC1 Gene: Construction of Saccharomyces cescerevisiae Mutant with Overexpression of PDC1 Gene","authors":"C. Ke, Bisha Wu, Qin Shao, Wei Yang, Jianzhong Huang","doi":"10.3724/SP.J.1145.2013.00704","DOIUrl":"https://doi.org/10.3724/SP.J.1145.2013.00704","url":null,"abstract":"","PeriodicalId":39895,"journal":{"name":"应用与环境生物学报","volume":"19 1","pages":"704-708"},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69812541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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