Pub Date : 2019-08-08DOI: 10.32448/ENTUPDATES.568049
Mustafa Daloğlu, K. Güney
Objective: The aim of this study was to investigate the incidence and severity of Frey’s syndrome (FS) among parotidectomy patients and to assess the impact of parotidectomy sequelae on quality of life (QoL). Methods: In total, 43 patients (21 males, 22 females) who underwent parotidectomy were included in this study. Iodine-starch test was applied to identify the presence and the severity of FS. The results were evaluated using a previously described grading system. Patients’ perceptions of parotidectomy sequelae were graded to identify their impact on QoL and also if there is an association between the extent of parotidectomy, the time elapsed after surgery and the severity of symptoms. Results: FS was diagnosed in 18 (41.8%) patients, 10 of which (23.3%) reported having FS symptoms. Of all patients who were diagnosed with FS, 11 (61.2%) patients’ symptoms were classified as mild, whereas 7 (38.8%) were classified as severe. There was no significant correlation between the incidence of FS and the time between surgery and diagnosis, or the extent of parotidectomy. Among all symptoms reported by patients, FS was the sequelae that caused the most discomfort after parotidectomy. Conclusion: The negative impact of FS on QoL is restricted to cases with severe symptoms, the incidence of which is rare and independent of the extent of surgery. To avoid overtreatment, true incidence of FS remains to be identified in study samples from different centers. By this means, clinicians can make more accurate definitions for indications of preventive procedures as well as treatment approaches.
{"title":"THE INCIDENCE AND SEVERITY OF FREY’S SYNDROME AFTER PAROTIDECTOMY: A RETROSPECTIVE STUDY","authors":"Mustafa Daloğlu, K. Güney","doi":"10.32448/ENTUPDATES.568049","DOIUrl":"https://doi.org/10.32448/ENTUPDATES.568049","url":null,"abstract":"Objective: The aim of this study was to investigate the incidence and severity of Frey’s syndrome (FS) among parotidectomy patients and to assess the impact of parotidectomy sequelae on quality of life (QoL). Methods: In total, 43 patients (21 males, 22 females) who underwent parotidectomy were included in this study. Iodine-starch test was applied to identify the presence and the severity of FS. The results were evaluated using a previously described grading system. Patients’ perceptions of parotidectomy sequelae were graded to identify their impact on QoL and also if there is an association between the extent of parotidectomy, the time elapsed after surgery and the severity of symptoms. Results: FS was diagnosed in 18 (41.8%) patients, 10 of which (23.3%) reported having FS symptoms. Of all patients who were diagnosed with FS, 11 (61.2%) patients’ symptoms were classified as mild, whereas 7 (38.8%) were classified as severe. There was no significant correlation between the incidence of FS and the time between surgery and diagnosis, or the extent of parotidectomy. Among all symptoms reported by patients, FS was the sequelae that caused the most discomfort after parotidectomy. Conclusion: The negative impact of FS on QoL is restricted to cases with severe symptoms, the incidence of which is rare and independent of the extent of surgery. To avoid overtreatment, true incidence of FS remains to be identified in study samples from different centers. By this means, clinicians can make more accurate definitions for indications of preventive procedures as well as treatment approaches.","PeriodicalId":41744,"journal":{"name":"ENT Updates","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48078605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-08DOI: 10.32448/ENTUPDATES.569464
Ş. Inanç, Didem Keleş, G. Eskiizmir, Y. Başbınar, G. Oktay
Objective: To assess the effects of Metformin, Dichloroacetate (DCA) and their combination on cell viability in oral squamous cell carcinoma, UPCI-SCC-131 cell line. Methods: UPCI-SCC-131 cells were plated in 96 E-plate (1x104 cells/well) and were treated with Metformin (1-16mM) and/or DCA (15-120mM) for 24-48-72h. xCELLigence SP system was used to monitor real time cell viability. In addition, drug combination index was analyzed with CompuSyn software according to Chou-Talalay method. Results: Half-maximal inhibitory concentrations (IC50) of Metformin and DCA were found to be 3mM and 23mM, respectively, for 72 hours. CI values (0.76-0.80) in all combination groups below 1 indicated that Metformin/DCA combination had a moderate synergistic effect on cell viability in UPCI-SCC-131 cells. Discussion: Metformin/DCA combination synergistically decreased the cell viability of UPCI-SCC-131 cells. Therefore, a combined application of Metformin and DCA may be considered as a candidate therapy for the “drug repositioning” of the treatment of oral cavity cancer.
{"title":"METFORMIN AND DICHOLOROACETATE COMBINATION EXERT A SYNERGISTIC EFFECT ON CELL VIABILITY OF ORAL SQUAMOUS CELL CARCINOMA","authors":"Ş. Inanç, Didem Keleş, G. Eskiizmir, Y. Başbınar, G. Oktay","doi":"10.32448/ENTUPDATES.569464","DOIUrl":"https://doi.org/10.32448/ENTUPDATES.569464","url":null,"abstract":"Objective: To assess the effects of Metformin, Dichloroacetate (DCA) and their combination on cell viability in oral squamous cell carcinoma, UPCI-SCC-131 cell line. Methods: UPCI-SCC-131 cells were plated in 96 E-plate (1x104 cells/well) and were treated with Metformin (1-16mM) and/or DCA (15-120mM) for 24-48-72h. xCELLigence SP system was used to monitor real time cell viability. In addition, drug combination index was analyzed with CompuSyn software according to Chou-Talalay method. Results: Half-maximal inhibitory concentrations (IC50) of Metformin and DCA were found to be 3mM and 23mM, respectively, for 72 hours. CI values (0.76-0.80) in all combination groups below 1 indicated that Metformin/DCA combination had a moderate synergistic effect on cell viability in UPCI-SCC-131 cells. Discussion: Metformin/DCA combination synergistically decreased the cell viability of UPCI-SCC-131 cells. Therefore, a combined application of Metformin and DCA may be considered as a candidate therapy for the “drug repositioning” of the treatment of oral cavity cancer.","PeriodicalId":41744,"journal":{"name":"ENT Updates","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42592940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-08DOI: 10.32448/ENTUPDATES.595445
I. Salcan, S. Salcan, H. Erdem, S. Müderris
Objective: Allergic rhinitis (AR) is one of the most frequently encountered allergic disorders and has a typical prevalence of between 18 and 40%.The factors which underlie allergic disorders may vary since they are linked to climate, geography, different social conditions and genetic makeup. The objective of our research was to investigate any connections between polymorphism in the serotonin transporter gene and allergic rhinitis. Methods: This study involved the participation of two groups aged between 15 and 60 years of age who attended the ENT clinic of the Medical Faculty at Erzincan University: 100 individuals with allergic rhinitis plus a control group of 200 individuals who lacked the condition. The AR cases were established on the basis of history, physical examination and skin prick testing. Peripheral blood samples were taken from both groups and subjected to genetic analyses. The analysis utilised a 5-HTT Taqman® primer as genetic probe. The Chi-squared method was utilised for statistical analysis. Results: 129 of the participants were female (64.5%) and 71 (35.5%) male. The average age of those participating was 34.5 ± 14.6 (range: 20-65) years. In reference to the sex distribution of the patients, 79% of the patients were female. There was a significant difference between the groups according to sex (p <0.01). No significant differences were noted between the patient and control groups when compared according to genetic polymorphism or place of residence. In 61% of the allergic rhinitis sufferers, their symptoms were seasonal. The incidence of AR was higher in both females and in those living in an urban rather than rural setting. When the polymorphism is examined in both the patient and control groups, the most common type is the mutant type. In both the cases and controls, when the polymorphism was investigated, the most frequently seen type was the Mutant variety and no significant difference in frequency was noted according to sex or place of residence. Conclusion: In this study, when the cases and controls were compared in terms of the polymorphism in the rs35521 serotonin transporter gene, there was no difference at the level of statistical significance between the frequency of occurrence of the mutant or heterozygote form of the gene.
{"title":"An Investigation of Genetic Polymorphism In The Rs35521 Serotonin Transporter Gene In Allergic Rhinitis","authors":"I. Salcan, S. Salcan, H. Erdem, S. Müderris","doi":"10.32448/ENTUPDATES.595445","DOIUrl":"https://doi.org/10.32448/ENTUPDATES.595445","url":null,"abstract":"Objective: Allergic rhinitis (AR) is one of the most frequently encountered allergic disorders and has a typical prevalence of between 18 and 40%.The factors which underlie allergic disorders may vary since they are linked to climate, geography, different social conditions and genetic makeup. The objective of our research was to investigate any connections between polymorphism in the serotonin transporter gene and allergic rhinitis. Methods: This study involved the participation of two groups aged between 15 and 60 years of age who attended the ENT clinic of the Medical Faculty at Erzincan University: 100 individuals with allergic rhinitis plus a control group of 200 individuals who lacked the condition. The AR cases were established on the basis of history, physical examination and skin prick testing. Peripheral blood samples were taken from both groups and subjected to genetic analyses. The analysis utilised a 5-HTT Taqman® primer as genetic probe. The Chi-squared method was utilised for statistical analysis. Results: 129 of the participants were female (64.5%) and 71 (35.5%) male. The average age of those participating was 34.5 ± 14.6 (range: 20-65) years. In reference to the sex distribution of the patients, 79% of the patients were female. There was a significant difference between the groups according to sex (p <0.01). No significant differences were noted between the patient and control groups when compared according to genetic polymorphism or place of residence. In 61% of the allergic rhinitis sufferers, their symptoms were seasonal. The incidence of AR was higher in both females and in those living in an urban rather than rural setting. When the polymorphism is examined in both the patient and control groups, the most common type is the mutant type. In both the cases and controls, when the polymorphism was investigated, the most frequently seen type was the Mutant variety and no significant difference in frequency was noted according to sex or place of residence. Conclusion: In this study, when the cases and controls were compared in terms of the polymorphism in the rs35521 serotonin transporter gene, there was no difference at the level of statistical significance between the frequency of occurrence of the mutant or heterozygote form of the gene.","PeriodicalId":41744,"journal":{"name":"ENT Updates","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45600128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-08DOI: 10.32448/ENTUPDATES.595446
S. Erkan, B. Tuhanioğlu
Introduction: Hearing aids are electronic devices used by individuals with hearing loss to improve their quality of life. It is more useful to use bilateral hearing aids. Thus, the localization of the sound is better determined and an additional 6-10 dB increase in sound intensity can be achieved. In our study, we compared the satisfaction level of patients with hearing loss who used unilateral or bilateral devices. Methods: Patients with bilateral sensorineural hearing loss and using unilateral or bilateral hearing aids for at least 3 months and whose hearing loss cannot be treated with medical or surgical treatments were included. International Outcome Inventory for Hearing Aids Turkish Version (IOI-HA- R) was administered to them. Results: Matched data were used to standardize age and gender between the groups. In high school bilateral fitting increased. There was no difference between the groups in terms of hearing pure tone thresholds and speech discriminations. There was a significant difference between the groups in terms of the mean score of question 5 (p=0.047), question 7 (p=0.022) and mean total scale (p=0.028) in IOI-HA-TR. Conclusion: As a result, we found that the satisfaction level of bilateral hearing aid users was higher. Bilateral hearing aid use has a positive effect on the social life of patients and increases their quality of life.
{"title":"Age And Sex Matched Data- Two Ears Improved Social Interaction And Quality Of Life","authors":"S. Erkan, B. Tuhanioğlu","doi":"10.32448/ENTUPDATES.595446","DOIUrl":"https://doi.org/10.32448/ENTUPDATES.595446","url":null,"abstract":"Introduction: Hearing aids are electronic devices used by individuals with hearing loss to improve their quality of life. It is more useful to use bilateral hearing aids. Thus, the localization of the sound is better determined and an additional 6-10 dB increase in sound intensity can be achieved. In our study, we compared the satisfaction level of patients with hearing loss who used unilateral or bilateral devices. Methods: Patients with bilateral sensorineural hearing loss and using unilateral or bilateral hearing aids for at least 3 months and whose hearing loss cannot be treated with medical or surgical treatments were included. International Outcome Inventory for Hearing Aids Turkish Version (IOI-HA- R) was administered to them. Results: Matched data were used to standardize age and gender between the groups. In high school bilateral fitting increased. There was no difference between the groups in terms of hearing pure tone thresholds and speech discriminations. There was a significant difference between the groups in terms of the mean score of question 5 (p=0.047), question 7 (p=0.022) and mean total scale (p=0.028) in IOI-HA-TR. Conclusion: As a result, we found that the satisfaction level of bilateral hearing aid users was higher. Bilateral hearing aid use has a positive effect on the social life of patients and increases their quality of life.","PeriodicalId":41744,"journal":{"name":"ENT Updates","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48058769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-08DOI: 10.32448/ENTUPDATES.596457
A. Kılavuz, Ali Bayram
Objective: Drug induced sleep endoscopy (DISE) is a valuable tool which is used in the diagnosis of obstructive sleep apnea (OUA). The aim of this study is to evaluate inter-rater and intra-rater consistency of DISE. Methods: 36 OSA patients with Apnea-hypopne index>5 included in this study. DISE was performed and recorded digitally for all patients, by the first author (OA1). VOTE scores were noted to procedure report in patients’ charts. Video records of DISE were blindly evaluated six months after the last procedure, by observer 1 for the second time (OA2) and by observer 2 (OB) for the first time. DISE was evaluated by using VOTE classification. OA1 and OA2 scores were compared to determine intra-rater reliability and OA2 and OB scores were compared to determine inter- rater reliability. Results: Inter-rater consistency of DISE was poor to good. Highest consistency rate was found in velum at anteroposterior configuration, while the lowest was found in the same level at lateral configuration. Intra-rater consistency of DISE was moderate to excellent. Highest consistency rate was found in epiglottis at lateral configuration, while the lowest was found in oropharynx level. Conclusion: OSA is condition with possible serious complications. DISE is a tool that could change the course of treatment in OSA. The validity of DISE is quite acceptable although a golden standard classification tool could enable us to “speak the exact same language” and will surely increase the diagnostic success of DISE.
{"title":"Intra-rater and Inter-rater consistency of drug induced sleep endoscopy","authors":"A. Kılavuz, Ali Bayram","doi":"10.32448/ENTUPDATES.596457","DOIUrl":"https://doi.org/10.32448/ENTUPDATES.596457","url":null,"abstract":"Objective: Drug induced sleep endoscopy (DISE) is a valuable tool which is used in the diagnosis of obstructive sleep apnea (OUA). The aim of this study is to evaluate inter-rater and intra-rater consistency of DISE. Methods: 36 OSA patients with Apnea-hypopne index>5 included in this study. DISE was performed and recorded digitally for all patients, by the first author (OA1). VOTE scores were noted to procedure report in patients’ charts. Video records of DISE were blindly evaluated six months after the last procedure, by observer 1 for the second time (OA2) and by observer 2 (OB) for the first time. DISE was evaluated by using VOTE classification. OA1 and OA2 scores were compared to determine intra-rater reliability and OA2 and OB scores were compared to determine inter- rater reliability. Results: Inter-rater consistency of DISE was poor to good. Highest consistency rate was found in velum at anteroposterior configuration, while the lowest was found in the same level at lateral configuration. Intra-rater consistency of DISE was moderate to excellent. Highest consistency rate was found in epiglottis at lateral configuration, while the lowest was found in oropharynx level. Conclusion: OSA is condition with possible serious complications. DISE is a tool that could change the course of treatment in OSA. The validity of DISE is quite acceptable although a golden standard classification tool could enable us to “speak the exact same language” and will surely increase the diagnostic success of DISE.","PeriodicalId":41744,"journal":{"name":"ENT Updates","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47646813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-08DOI: 10.32448/ENTUPDATES.594625
Ali Bayram, D. Marchioni, Kevin Peng, I. Moon, C. Cingi
Since the introduction of endoscopic ear surgery (EES) in the 1990s, endoscopic systems have been utilized in a number of ear surgeries including myringoplasty and tympanoplasty. Endoscopic tympanoplasty (ET) is described as a minimally invasive surgery for surgical repair of the tympanic membrane (TM) with comparable graft survival and hearing outcomes to conventional microscopic surgery (CMT). In the present review, we aimed to outline the surgical benefits and potential drawbacks of ET with clinical outcomes in order to determine whether ET constitutes a proper alternative to CMT.
{"title":"How do you perform your tympanoplasty, endoscopically or microscopically?","authors":"Ali Bayram, D. Marchioni, Kevin Peng, I. Moon, C. Cingi","doi":"10.32448/ENTUPDATES.594625","DOIUrl":"https://doi.org/10.32448/ENTUPDATES.594625","url":null,"abstract":"Since the introduction of endoscopic ear surgery (EES) in the 1990s, endoscopic systems have been utilized in a number of ear surgeries including myringoplasty and tympanoplasty. Endoscopic tympanoplasty (ET) is described as a minimally invasive surgery for surgical repair of the tympanic membrane (TM) with comparable graft survival and hearing outcomes to conventional microscopic surgery (CMT). In the present review, we aimed to outline the surgical benefits and potential drawbacks of ET with clinical outcomes in order to determine whether ET constitutes a proper alternative to CMT.","PeriodicalId":41744,"journal":{"name":"ENT Updates","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43264298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-08DOI: 10.32448/ENTUPDATES.587285
Kadri Ila, Emre Soylemez, N. Yilmaz, A. Eshraghi
Objective: Objective: Chronic rhinosinusitis (CRS) is inflammation of the nasal mucosa and paranasal sinuses. Subjective complaint of dizziness can be observed in patients with CRS. However, a review of the literature did not reveal any prospective studies that have used videonystagmography (VNG) to examine the vestibular system of patients with CRS. The goal of this study is the evaluation of the vestibular system of CRS patients. Methods: The study comprised 30 patients with CRS and 30 control subjects. The vestibular system was evaluated through VNG. The association with vestibular function was evaluated according to the Lund-Mackay score, the Sino-Nasal Outcome Test-22 (SNOT-22) score, and the existence of nasal polyps. Results: Abnormal caloric test results were observed in eight (26.6%) of the 30 CRS patients. The caloric test results were normal for the control group. The oculomotor test results with VNG were normal for the CRS and control groups. The abnormal caloric results were higher for the CRS with nasal polyp patients than for the CRS without nasal polyp patients. The VNG showed no correlation among the SNOT-22 scores and the Lund-Mackay scores in patients with CRS. Conclusion: Abnormal caloric responses can be observed in patients with CRS with nasal polyps.
目的：慢性鼻窦炎（CRS）是鼻黏膜和鼻窦的炎症。CRS患者可观察到头晕的主观主诉。然而，对文献的回顾并没有揭示任何使用视频眼球震颤描记术（VNG）检查CRS患者前庭系统的前瞻性研究。本研究的目的是评估CRS患者的前庭系统。方法：该研究包括30名CRS患者和30名对照受试者。前庭系统通过VNG进行评估。根据Lund-Mackay评分、Sino-Nasal Result Test-22（SNOT-22）评分和鼻息肉的存在来评估与前庭功能的相关性。结果：30例CRS患者中有8例（26.6%）出现异常热量测试结果。对照组的热量测试结果正常。CRS组和对照组的VNG眼动测试结果正常。有鼻息肉的CRS患者的异常热量结果高于没有鼻息肉的患者。CRS患者的VNG显示SNOT-22评分和Lund-Mackay评分之间没有相关性。结论：CRS合并鼻息肉患者可观察到异常的热反应。
{"title":"Association Between Chronic Rhinosinusitis and The Vestibular System","authors":"Kadri Ila, Emre Soylemez, N. Yilmaz, A. Eshraghi","doi":"10.32448/ENTUPDATES.587285","DOIUrl":"https://doi.org/10.32448/ENTUPDATES.587285","url":null,"abstract":"Objective: Objective: Chronic rhinosinusitis (CRS) is inflammation of the nasal mucosa and paranasal sinuses. Subjective complaint of dizziness can be observed in patients with CRS. However, a review of the literature did not reveal any prospective studies that have used videonystagmography (VNG) to examine the vestibular system of patients with CRS. The goal of this study is the evaluation of the vestibular system of CRS patients. Methods: The study comprised 30 patients with CRS and 30 control subjects. The vestibular system was evaluated through VNG. The association with vestibular function was evaluated according to the Lund-Mackay score, the Sino-Nasal Outcome Test-22 (SNOT-22) score, and the existence of nasal polyps. Results: Abnormal caloric test results were observed in eight (26.6%) of the 30 CRS patients. The caloric test results were normal for the control group. The oculomotor test results with VNG were normal for the CRS and control groups. The abnormal caloric results were higher for the CRS with nasal polyp patients than for the CRS without nasal polyp patients. The VNG showed no correlation among the SNOT-22 scores and the Lund-Mackay scores in patients with CRS. Conclusion: Abnormal caloric responses can be observed in patients with CRS with nasal polyps.","PeriodicalId":41744,"journal":{"name":"ENT Updates","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46394166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-08DOI: 10.32448/ENTUPDATES.578602
A. Kepekçi, Merve Seda Ibisoglu, S. Yılmaz, Cenk Kıg
Objective: Inter-retrotransposon polymorphism Polymerase Chain Reaction (IRAP-PCR) technique allows for detecting insertional polymorphisms via amplification of the DNA fragment between two retrotransposons in plant genomes. However, this method has not been reported to be used for analyzing human samples to date. Recently, Human Endogenous Retrovirus (HERV) polymorphisms gained interest due to their potential effect on pathophysiology of certain diseases. Nevertheless, the association between HERV polymorphisms and the risk for developing nasal polyposis (NP) has not been studied. In this study, we aimed to investigate whether or not IRAP-PCR could be performed in nasal swab samples for comparing HERV polymorphisms in different nasal mucosal samples. Methods: Nasal swab samples from 16 patients were used for DNA isolation. These DNA samples were used as templates for IRAP PCR of HERV-K6, HERV-K11, HERV-L1 and HERV-L2 and PCR products were analyzed by agarose gel electrophoresis. Results: Nasal swab samples yielded enough DNA material for successfully performing IRAP-PCR. We obtained specific banding patterns the three out of four HERV sequences tested in this study. No polymorphisms was detected between samples from different patients. Similarly, polymorphic bands was not detected between the polyps or nasal mucosal swab samples obtained from the same patient. Conclusion: We have, for the first time, shown that IRAPPCR can be performed in nasal swabs. Our findings suggest that this technique can serve as an inexpensive and effective screening tool for investigating links between nasal mucosal diseases and HERV polymorphisms such as nasal polyposis.
{"title":"IRAP-PCR As A Tool For Screening HERV Polymorphisms In Nasal Mucosal Swabs","authors":"A. Kepekçi, Merve Seda Ibisoglu, S. Yılmaz, Cenk Kıg","doi":"10.32448/ENTUPDATES.578602","DOIUrl":"https://doi.org/10.32448/ENTUPDATES.578602","url":null,"abstract":"Objective: Inter-retrotransposon polymorphism Polymerase Chain Reaction (IRAP-PCR) technique allows for detecting insertional polymorphisms via amplification of the DNA fragment between two retrotransposons in plant genomes. However, this method has not been reported to be used for analyzing human samples to date. Recently, Human Endogenous Retrovirus (HERV) polymorphisms gained interest due to their potential effect on pathophysiology of certain diseases. Nevertheless, the association between HERV polymorphisms and the risk for developing nasal polyposis (NP) has not been studied. In this study, we aimed to investigate whether or not IRAP-PCR could be performed in nasal swab samples for comparing HERV polymorphisms in different nasal mucosal samples. Methods: Nasal swab samples from 16 patients were used for DNA isolation. These DNA samples were used as templates for IRAP PCR of HERV-K6, HERV-K11, HERV-L1 and HERV-L2 and PCR products were analyzed by agarose gel electrophoresis. Results: Nasal swab samples yielded enough DNA material for successfully performing IRAP-PCR. We obtained specific banding patterns the three out of four HERV sequences tested in this study. No polymorphisms was detected between samples from different patients. Similarly, polymorphic bands was not detected between the polyps or nasal mucosal swab samples obtained from the same patient. Conclusion: We have, for the first time, shown that IRAPPCR can be performed in nasal swabs. Our findings suggest that this technique can serve as an inexpensive and effective screening tool for investigating links between nasal mucosal diseases and HERV polymorphisms such as nasal polyposis.","PeriodicalId":41744,"journal":{"name":"ENT Updates","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49105685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.32448/ENTUPDATES.574363
Zafer Volkan Gökçe, S. Öztürk, Ceki Paltura
Objective: To demonstrate the effects of rheumatoid arthritis (RA) on hearing with audiometric and tympanometric measurements, and to reveal its relationship with the disease in comparison with healthy volunteers. Methods: The study included 30 RA patients and 30 healthy controls. Demographic findings such as age, height, weight, onset of complaint (year), time elapsed since diagnosis, previous surgery, accompanying systemic disease, drugs used, family history, habits of smoking and alcohol use were questioned for both groups. Tympanometry, pure voice audiometry and otoacoustic Emission tests were performed. Results: The hearing thresholds in the airway conduction in RA group were significantly higher in all frequencies (0.25-6 kHz) than in the control group. When bone conduction hearing thresholds were compared, RA group was more affected at only 4 kHz and left ear (p=0.006). When air-bone conduction differences (AB-gap) were examined, the differences in the RA group at 0.5, 1 and 4 kHz were statistically significant. When tympanometry and acoustic reflex measurement results were evaluated, there was no statistically significant difference between groups. In the transient OAE test, 3 (10 %) patients were in the RA and 2 (6.6 %) patients in the control group (p=0.064). Conclusion: Conductive hearing loss was significantly more frequent in patients with RA than in healthy individuals, whereas bone conduction was affected partially at high frequencies; whereas stapes reflex, compliance and pressure values were not significantly affected.
{"title":"Evaluation of hearing in patients with rheumatoid arthritis compared with controls","authors":"Zafer Volkan Gökçe, S. Öztürk, Ceki Paltura","doi":"10.32448/ENTUPDATES.574363","DOIUrl":"https://doi.org/10.32448/ENTUPDATES.574363","url":null,"abstract":"Objective: To demonstrate the effects of rheumatoid arthritis (RA) on hearing with audiometric and tympanometric measurements, and to reveal its relationship with the disease in comparison with healthy volunteers. Methods: The study included 30 RA patients and 30 healthy controls. Demographic findings such as age, height, weight, onset of complaint (year), time elapsed since diagnosis, previous surgery, accompanying systemic disease, drugs used, family history, habits of smoking and alcohol use were questioned for both groups. Tympanometry, pure voice audiometry and otoacoustic Emission tests were performed. Results: The hearing thresholds in the airway conduction in RA group were significantly higher in all frequencies (0.25-6 kHz) than in the control group. When bone conduction hearing thresholds were compared, RA group was more affected at only 4 kHz and left ear (p=0.006). When air-bone conduction differences (AB-gap) were examined, the differences in the RA group at 0.5, 1 and 4 kHz were statistically significant. When tympanometry and acoustic reflex measurement results were evaluated, there was no statistically significant difference between groups. In the transient OAE test, 3 (10 %) patients were in the RA and 2 (6.6 %) patients in the control group (p=0.064). Conclusion: Conductive hearing loss was significantly more frequent in patients with RA than in healthy individuals, whereas bone conduction was affected partially at high frequencies; whereas stapes reflex, compliance and pressure values were not significantly affected.","PeriodicalId":41744,"journal":{"name":"ENT Updates","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41946270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.32448/ENTUPDATES.570136
S. Ertugrul, Emre Soylemez
Objective: The aim of this study was to determine the factors affecting the success of vestibular rehabilitation therapy (VRT) in patients with idiopathic unilateral vestibular hypofunction (UVH) and idiopathic bilateral vestibular hypofunction (BVH). Methods: 30 patients with idiopathic UVH and 30 patients with idiopathic BVH were included in this prospective study. Visual analog scale (VAS) was used to evaluate the severity of the patients’ complaints of dizziness. The duration of the complaint of dizziness was recorded. All patients underwent the Dizziness Handicap Inventory (DHI) before and after VRT. The severity, duration, and localization (unilateral/bilateral) of the vestibular hypofunction were compared with the efficacy of VRT. Results: A significant decrease in DHI scores was observed after VRT for both, UVH and BVH patients, as compared to their pre-VRT scores (p<0.001). There was no significant difference in the efficacy of vestibular rehabilitation therapy in patients with UVH and BVH (p=0.09). As the VAS scores increased and the duration of the complaint lengthened, the efficacy of VRT decreased significantly (p<0.001/r=5.6, p=0.016/r=3.1, respectively). Conclusion : VRT is an effective treatment for the relief of symptoms in both UVH and BVH patients. Unilateral or bilateral vestibular hypofunction does not affect the efficacy of VRT, whereas prolonged or severe symptoms of dizziness affect the effectiveness of VRT negatively. In idiopathic vestibular hypofunction patients with long-standing and/or severe dizziness complaints, VRT should be initiated immediately and continued longer.
{"title":"Investigation of the factors affecting the success of vestibular rehabilitation therapy in patients with idiopathic unilateral vestibular hypofunction and idiopathic bilateral vestibular hypofunction","authors":"S. Ertugrul, Emre Soylemez","doi":"10.32448/ENTUPDATES.570136","DOIUrl":"https://doi.org/10.32448/ENTUPDATES.570136","url":null,"abstract":"Objective: The aim of this study was to determine the factors affecting the success of vestibular rehabilitation therapy (VRT) in patients with idiopathic unilateral vestibular hypofunction (UVH) and idiopathic bilateral vestibular hypofunction (BVH). Methods: 30 patients with idiopathic UVH and 30 patients with idiopathic BVH were included in this prospective study. Visual analog scale (VAS) was used to evaluate the severity of the patients’ complaints of dizziness. The duration of the complaint of dizziness was recorded. All patients underwent the Dizziness Handicap Inventory (DHI) before and after VRT. The severity, duration, and localization (unilateral/bilateral) of the vestibular hypofunction were compared with the efficacy of VRT. Results: A significant decrease in DHI scores was observed after VRT for both, UVH and BVH patients, as compared to their pre-VRT scores (p<0.001). There was no significant difference in the efficacy of vestibular rehabilitation therapy in patients with UVH and BVH (p=0.09). As the VAS scores increased and the duration of the complaint lengthened, the efficacy of VRT decreased significantly (p<0.001/r=5.6, p=0.016/r=3.1, respectively). Conclusion : VRT is an effective treatment for the relief of symptoms in both UVH and BVH patients. Unilateral or bilateral vestibular hypofunction does not affect the efficacy of VRT, whereas prolonged or severe symptoms of dizziness affect the effectiveness of VRT negatively. In idiopathic vestibular hypofunction patients with long-standing and/or severe dizziness complaints, VRT should be initiated immediately and continued longer.","PeriodicalId":41744,"journal":{"name":"ENT Updates","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45933083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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