Pub Date : 2022-04-01DOI: 10.14427/jipai.2022.2.65
A. Sukalo, N. Strogaya
Juvenile idiopathic arthritis (JIA) is a systemic chronic disease. One of the extra-articular manifestations of JIA are hematological changes. The most common is anemia. The aim of the study was to explore the indicators of the red blood cells and the state of iron metabolism in children with JIA, depending on the form and duration of the disease. As a result of the study, it was found that the decrease in the iron content in the blood serum is due not only to the true iron deficiency state, but also to the redistribution of iron reserves against the background of chronic autoimmune inflammation.
{"title":"Features of anemic syndrome in juvenile idiopathic arthritis","authors":"A. Sukalo, N. Strogaya","doi":"10.14427/jipai.2022.2.65","DOIUrl":"https://doi.org/10.14427/jipai.2022.2.65","url":null,"abstract":"Juvenile idiopathic arthritis (JIA) is a systemic chronic disease. One of the extra-articular manifestations of JIA are hematological changes. The most common is anemia. The aim of the study was to explore the indicators of the red blood cells and the state of iron metabolism in children with JIA, depending on the form and duration of the disease. As a result of the study, it was found that the decrease in the iron content in the blood serum is due not only to the true iron deficiency state, but also to the redistribution of iron reserves against the background of chronic autoimmune inflammation.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124045664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.14427/jipai.2022.2.74
N. Shipilova, I. Sergeeva
Chronic kidney disease (CKD) occurs in 15% of adults. The aim of the study was to evaluate the occurrence and clinical features of onychomycosis in patients with CKD receiving hemodialysis. A study comprised 25 patients (51±22 years) with CKD and nail changes with chronic renal failure under haemodialysis and 25 patients (53.6 ± 26.4 years) without CKD. For CKD, «yellow nails» are typical – in 17 (68%), without CKD – longitudinal stripes – in 16 (64%). Onychomycosis was diagnosed in 32% of patients with CKD and 64% without CKD. Out of 8 patients with diabetes mellitus and CKD, 7 had onychomycosis, 1 patient with CKD and onychomycosis did not have diabetes mellitus. For patients with CKD, the OSI index was 12 [9; 14], SCIO 10 [8; 12], without CKD: OSI – 16 [12; 17], SCIO – 12 [10; 15]. Onychomycosis in patients with CKD is less common than in patients without CKD, which indicates a lesser significance of nail infection with fungi in the development of their changes in patients under hemodialysis.
慢性肾脏疾病(CKD)发生在15%的成年人中。本研究的目的是评估CKD患者接受血液透析的甲癣的发生和临床特征。该研究包括25例(51±22岁)CKD合并指甲改变合并血液透析慢性肾衰竭患者和25例(53.6±26.4岁)非CKD患者。对于CKD，“黄指甲”是典型的- 17例(68%)，没有CKD -纵向条纹- 16例(64%)。32%的CKD患者和64%的非CKD患者被诊断为甲癣。8例糖尿病合并慢性肾病患者中，7例合并甲真菌病，1例合并慢性肾病合并甲真菌病不合并糖尿病。对于CKD患者，OSI指数为12 [9;[14]， [8];[12]，无CKD: OSI - 16 [12;[17]、[10];15)。CKD患者的甲真菌病比非CKD患者少见，这表明在血液透析患者中，指甲感染真菌对其变化的发展意义较小。
{"title":"Onychomycosis in patients with chronic kidney disease","authors":"N. Shipilova, I. Sergeeva","doi":"10.14427/jipai.2022.2.74","DOIUrl":"https://doi.org/10.14427/jipai.2022.2.74","url":null,"abstract":"Chronic kidney disease (CKD) occurs in 15% of adults. The aim of the study was to evaluate the occurrence and clinical features of onychomycosis in patients with CKD receiving hemodialysis. A study comprised 25 patients (51±22 years) with CKD and nail changes with chronic renal failure under haemodialysis and 25 patients (53.6 ± 26.4 years) without CKD. For CKD, «yellow nails» are typical – in 17 (68%), without CKD – longitudinal stripes – in 16 (64%). Onychomycosis was diagnosed in 32% of patients with CKD and 64% without CKD. Out of 8 patients with diabetes mellitus and CKD, 7 had onychomycosis, 1 patient with CKD and onychomycosis did not have diabetes mellitus. For patients with CKD, the OSI index was 12 [9; 14], SCIO 10 [8; 12], without CKD: OSI – 16 [12; 17], SCIO – 12 [10; 15]. Onychomycosis in patients with CKD is less common than in patients without CKD, which indicates a lesser significance of nail infection with fungi in the development of their changes in patients under hemodialysis.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130187894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.14427/jipai.2022.2.45
O. Ishchenko, A. Badyukov, T. Badyoukova, A. Zebentaev, A. Valynets
Background. Benign prostatic hyperplasia (BPH) is a histologically determined condition of the male body and is characterized by glandular-stromal multi-focal proliferation originating from the paraurethral and (or) transition zone of the prostate gland. Proliferative processes in the body are under the control of the immune system. Identifying and understanding the immunological aspects of prostatic hyperplasia can help control prostate tissue growth, improving men's quality of life. The aim of our study was to conduct a pilot study, assessment of the immune status of patients with benign prostatic hyperplasia. Materials and methods. The study included patients having stage 2 BPH (according to Guyon’s updated classification system (n=8)) and patients who did not have clinical and morphological manifestations of BPH. The subjects underwent standard examinations in accordance with the clinical protocol of the Ministry of Health of the Republic of Belarus. The immunogram indicators included: the main subpopulations of lymphocytes (CD3 T lymphocytes, CD4 T helper cells, CD8 T cytotoxic lymphocytes), the ratio of Th/Tc, CD19 B lymphocytes, the level of serum immunoglobulins IgG, IgM, IgA and circulating immunocomplexes (CIC), as well as phagocytic index and phagocytic number. Results. Patients with BPH had statistically lower levels in the number of phagocytosed units compared to the patients without BPH. Furthermore, patients with BPH have highly variable results for immune system indices. Thus, the ratio of Th/Tc ranged from 1,3 to 2,3 units, and the level of CIC – from 10 to more than 300 units. The variability of the results obtained allowed us to divide patients with BPH into two groups: first group (n=3) with normal indices for levels of CIC and for the ratio of Th/Tc; the second group (n=5) with the indices for levels of CIC and for the ratio of Th/Tc that significantly exceeded the reference values. None of the patients in the second group had a concomitant autoimmune disease. Conclusions. Patients with BPH have a dysfunction of the immune system. Even having such a small number of enrolled subjects, it is possible to form subtypes of patients with BPH – they are future phenotypes. The study aiming at finding immunological disorders in patients with benign prostatic hyperplasia should be continued.
{"title":"Heterogeneity of benign prostatic hyperplasia","authors":"O. Ishchenko, A. Badyukov, T. Badyoukova, A. Zebentaev, A. Valynets","doi":"10.14427/jipai.2022.2.45","DOIUrl":"https://doi.org/10.14427/jipai.2022.2.45","url":null,"abstract":"Background. Benign prostatic hyperplasia (BPH) is a histologically determined condition of the male body and is characterized by glandular-stromal multi-focal proliferation originating from the paraurethral and (or) transition zone of the prostate gland. Proliferative processes in the body are under the control of the immune system. Identifying and understanding the immunological aspects of prostatic hyperplasia can help control prostate tissue growth, improving men's quality of life. The aim of our study was to conduct a pilot study, assessment of the immune status of patients with benign prostatic hyperplasia. Materials and methods. The study included patients having stage 2 BPH (according to Guyon’s updated classification system (n=8)) and patients who did not have clinical and morphological manifestations of BPH. The subjects underwent standard examinations in accordance with the clinical protocol of the Ministry of Health of the Republic of Belarus. The immunogram indicators included: the main subpopulations of lymphocytes (CD3 T lymphocytes, CD4 T helper cells, CD8 T cytotoxic lymphocytes), the ratio of Th/Tc, CD19 B lymphocytes, the level of serum immunoglobulins IgG, IgM, IgA and circulating immunocomplexes (CIC), as well as phagocytic index and phagocytic number. Results. Patients with BPH had statistically lower levels in the number of phagocytosed units compared to the patients without BPH. Furthermore, patients with BPH have highly variable results for immune system indices. Thus, the ratio of Th/Tc ranged from 1,3 to 2,3 units, and the level of CIC – from 10 to more than 300 units. The variability of the results obtained allowed us to divide patients with BPH into two groups: first group (n=3) with normal indices for levels of CIC and for the ratio of Th/Tc; the second group (n=5) with the indices for levels of CIC and for the ratio of Th/Tc that significantly exceeded the reference values. None of the patients in the second group had a concomitant autoimmune disease. Conclusions. Patients with BPH have a dysfunction of the immune system. Even having such a small number of enrolled subjects, it is possible to form subtypes of patients with BPH – they are future phenotypes. The study aiming at finding immunological disorders in patients with benign prostatic hyperplasia should be continued.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"126 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127398262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.14427/jipai.2022.2.68
R. Prokhorenko, N. Aliakhnovich, A. Patryna
Professional sports is a field of human activity where all body systems function in the zone of absolute physiological limits of the body. Good physical fitness of athletes in general was established, with satisfactory indicators in 26%. Disorders in the metabolism of athletes were found, indicating a violation of recovery after physical exertion and injury: a decrease in the atherogenicity index in 70%, the level of uric acid and globulins in 12% of participants; an increase in total and direct bilirubin in 28-32%, creatine phosphokinase in 42%. Increased physical activity affected the T-cell link of immunity (the relative number of T-lymphocytes was reduced in 20%, CD4-T-lymphocytes – in 24%, active T-lymphocytes were increased in 12% of athletes; the immunoregulatory index was increased in 8%, decreased in 14%). The examined athletes were often ill with acute infections – 52% suffered from acute respiratory viral infections, including Covid-19, acute intestinal infections in the last 3 months, 84% – in the last 12 months. Athletes noted a feeling of difficulty breathing after physical exertion (20% – less than several times a week, 4% – several times a week), episodes of suffocation – 10% of athletes, that is, they were at risk of developing bronchial asthma. A direct correlation was found between blood glucose, liver parameters, calcium and creatiphosphokinase levels, and pulse and blood pressure indicators before and after the Roufier test. The levels of creatyphosphokinase and liver parameters were inversely correlated with the number of episodes of difficulty breathing.
{"title":"Correlation of physical fitness of CSС «Vitebsk» athletes and biochemical, immunological blood parameters","authors":"R. Prokhorenko, N. Aliakhnovich, A. Patryna","doi":"10.14427/jipai.2022.2.68","DOIUrl":"https://doi.org/10.14427/jipai.2022.2.68","url":null,"abstract":"Professional sports is a field of human activity where all body systems function in the zone of absolute physiological limits of the body. Good physical fitness of athletes in general was established, with satisfactory indicators in 26%. Disorders in the metabolism of athletes were found, indicating a violation of recovery after physical exertion and injury: a decrease in the atherogenicity index in 70%, the level of uric acid and globulins in 12% of participants; an increase in total and direct bilirubin in 28-32%, creatine phosphokinase in 42%. Increased physical activity affected the T-cell link of immunity (the relative number of T-lymphocytes was reduced in 20%, CD4-T-lymphocytes – in 24%, active T-lymphocytes were increased in 12% of athletes; the immunoregulatory index was increased in 8%, decreased in 14%). The examined athletes were often ill with acute infections – 52% suffered from acute respiratory viral infections, including Covid-19, acute intestinal infections in the last 3 months, 84% – in the last 12 months. Athletes noted a feeling of difficulty breathing after physical exertion (20% – less than several times a week, 4% – several times a week), episodes of suffocation – 10% of athletes, that is, they were at risk of developing bronchial asthma. A direct correlation was found between blood glucose, liver parameters, calcium and creatiphosphokinase levels, and pulse and blood pressure indicators before and after the Roufier test. The levels of creatyphosphokinase and liver parameters were inversely correlated with the number of episodes of difficulty breathing.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115961695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.14427/jipai.2022.2.58
T. Novikova, E. Dotsenko
In the last two decades, there has been an increase in allergic reactions caused by sensitization to fungi. This variant of hypersensitivity causes more severe allergic disease, frequent, prolonged exacerbations that are difficult to control. Therefore, the diagnosis of early stages of fungi allergy is important. The study demonstrates the use of a differentiated model in diagnosis of the allergic nature of a respiratory disease depending on the total serum Ig E level in patients with bronchial asthma and allergic rhinitis with fungi sensitization. A differentiated assessment of total serum Ig E upper normal level, corresponding to clinical respiratory disease manifestations, allows the test using as the first stage of allergy diagnosis: screening of patients with respiratory diseases at the initial appointment to select those patients who need a specific allergy testing, performed by allergologist.
{"title":"The role of total serum immunoglobulin E level in diagnosis of fungal allergy","authors":"T. Novikova, E. Dotsenko","doi":"10.14427/jipai.2022.2.58","DOIUrl":"https://doi.org/10.14427/jipai.2022.2.58","url":null,"abstract":"In the last two decades, there has been an increase in allergic reactions caused by sensitization to fungi. This variant of hypersensitivity causes more severe allergic disease, frequent, prolonged exacerbations that are difficult to control. Therefore, the diagnosis of early stages of fungi allergy is important. The study demonstrates the use of a differentiated model in diagnosis of the allergic nature of a respiratory disease depending on the total serum Ig E level in patients with bronchial asthma and allergic rhinitis with fungi sensitization. A differentiated assessment of total serum Ig E upper normal level, corresponding to clinical respiratory disease manifestations, allows the test using as the first stage of allergy diagnosis: screening of patients with respiratory diseases at the initial appointment to select those patients who need a specific allergy testing, performed by allergologist.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121573065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.14427/jipai.2022.2.38
N. Aliakhnovich, A. Skorobogatova, A. Gancharova
The effect of the white pigment titanium dioxide (TiO2) on the protein enzyme, the bacteriolytic component of the innate immune system, lysozyme, was evaluated. The effects of TiO2 in the form of nanoparticles, microparticles and particles used in the food industry at concentrations of 0.001-0.0001 mg/ml, corresponding to the calculated average daily intake and 10-fold dilution, were studied. It was found that a lower concentration of TiO2 (0.0001 mg/ml) of all particles types acts more efficiently, significantly reducing after 30 minutes, and completely blocking the own fluorescence of recombinant lysozyme after 60 minutes. TiO2 particles of three types at a higher concentration (0.001 mg/ml) completely block the intrinsic fluorescence of recombinant lysozyme after 60 minutes. After 30 minutes only microparticles cause a decrease in the intrinsic protein fluorescence. The inhibition of the bacteriolytic activity of recombinant lysozyme against the culture of K. pneumoniae under the action of nanoparticles, microparticles and particles of food TiO2 at concentrations of 0.001-0.0001 mg/ml was revealed.
{"title":"Titanium dioxide effect on bacteriolytic activity and intrinsic fluorescence of lysozyme","authors":"N. Aliakhnovich, A. Skorobogatova, A. Gancharova","doi":"10.14427/jipai.2022.2.38","DOIUrl":"https://doi.org/10.14427/jipai.2022.2.38","url":null,"abstract":"The effect of the white pigment titanium dioxide (TiO2) on the protein enzyme, the bacteriolytic component of the innate immune system, lysozyme, was evaluated. The effects of TiO2 in the form of nanoparticles, microparticles and particles used in the food industry at concentrations of 0.001-0.0001 mg/ml, corresponding to the calculated average daily intake and 10-fold dilution, were studied. It was found that a lower concentration of TiO2 (0.0001 mg/ml) of all particles types acts more efficiently, significantly reducing after 30 minutes, and completely blocking the own fluorescence of recombinant lysozyme after 60 minutes. TiO2 particles of three types at a higher concentration (0.001 mg/ml) completely block the intrinsic fluorescence of recombinant lysozyme after 60 minutes. After 30 minutes only microparticles cause a decrease in the intrinsic protein fluorescence. The inhibition of the bacteriolytic activity of recombinant lysozyme against the culture of K. pneumoniae under the action of nanoparticles, microparticles and particles of food TiO2 at concentrations of 0.001-0.0001 mg/ml was revealed.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127996507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.14427/jipai.2022.2.51
T. Sobolenko, O. Ishchenko, O. Zakharov, V. Laporevich
Acute macroglossia not associated with angioedema is a rare condition. We present a case of acute unilateral macroglossia of unclear etiology in a 46-year-old man. Tongue enlargement was accompanied by difficulty in talking, but did not result in upper airway obstruction. Angioedema of the tongue was excluded. The symptoms disappeared within one month. Cervical radiculopathy and COVID-19 infection are considered as probable causes of macroglossia.
{"title":"Non-angioedema-related macroglossia: case report","authors":"T. Sobolenko, O. Ishchenko, O. Zakharov, V. Laporevich","doi":"10.14427/jipai.2022.2.51","DOIUrl":"https://doi.org/10.14427/jipai.2022.2.51","url":null,"abstract":"Acute macroglossia not associated with angioedema is a rare condition. We present a case of acute unilateral macroglossia of unclear etiology in a 46-year-old man. Tongue enlargement was accompanied by difficulty in talking, but did not result in upper airway obstruction. Angioedema of the tongue was excluded. The symptoms disappeared within one month. Cervical radiculopathy and COVID-19 infection are considered as probable causes of macroglossia.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"289 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134428463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.14427/jipai.2022.2.28
S. Zhavoronok, I. Zadora, V. Davydov, T. Rogacheva, L. Anisko, G. Alatortseva, V. Simirsky, A. Shcherban, N. Sсhuka, N. Bayur, Y. Shebeko, J. Mitko
Due to the lack of national analogies a prototype of enzyme-linked immunosorbent assay (ELISA) test system was developed for the qualitative determination of antibodies class G to hepatitis E virus (HEV) based on the using of antigenic polypeptides ORF2 and ORF3 of the 3rd genotype (FSBSI «I.I. Mechnikov Research Institute of Vaccines and Serums», Russia). The results of experimental ELISA conducted in parallel with the reference test system «DS-ELISA-ANTI-HEV-G» (NPO Diagnostic Systems, Russia) are presented. The working concentrations of ORF2 and ORF3 antigens were 2.5 µg/mL and 0.25 µg/mL, respectively. They were tested with 200 donors and patients’ serum samples. The article reflects the determination of sensitivity, specificity and reproducibility results. The developed test system demonstrated high efficiency in the detection of specific anti-HEV-IgG.
{"title":"The use of ORF2 and ORF3 proteins for creating an enzyme-linked immunosorbent assay for viral hepatitis E","authors":"S. Zhavoronok, I. Zadora, V. Davydov, T. Rogacheva, L. Anisko, G. Alatortseva, V. Simirsky, A. Shcherban, N. Sсhuka, N. Bayur, Y. Shebeko, J. Mitko","doi":"10.14427/jipai.2022.2.28","DOIUrl":"https://doi.org/10.14427/jipai.2022.2.28","url":null,"abstract":"Due to the lack of national analogies a prototype of enzyme-linked immunosorbent assay (ELISA) test system was developed for the qualitative determination of antibodies class G to hepatitis E virus (HEV) based on the using of antigenic polypeptides ORF2 and ORF3 of the 3rd genotype (FSBSI «I.I. Mechnikov Research Institute of Vaccines and Serums», Russia). The results of experimental ELISA conducted in parallel with the reference test system «DS-ELISA-ANTI-HEV-G» (NPO Diagnostic Systems, Russia) are presented. The working concentrations of ORF2 and ORF3 antigens were 2.5 µg/mL and 0.25 µg/mL, respectively. They were tested with 200 donors and patients’ serum samples. The article reflects the determination of sensitivity, specificity and reproducibility results. The developed test system demonstrated high efficiency in the detection of specific anti-HEV-IgG.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130817534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Polyakova, I. Guryanova, M. Stegantseva, S. Sharapova, I. Sakovich, A. Kupchinskaya, S. Aleshkevich, Y. Zharankova, V. Kazak, T. Volodashchik, M. Belevtsev
Purpose. Evaluate the diagnostic significance of quantitative determination of T- and B-cell receptor DNA ring fragments in peripheral blood of patients for diagnosis of ataxia-teleangiectasia. Materials and methods. The subjects of the study were peripheral blood samples from 14 patients diagnosed with ataxia-teleangiectasia aged 4.9 (1.6; 14.4) years with mutations in the ATM gene and from 40 healthy children aged 11.0 (6.7; 14.8) years. Results and discussion. Patients with ataxia-teleangiectasia revealed a significant decrease in TREC and KREC values (p<0.05) compared to the control group of healthy children. Diagnostic sensitivity and specificity concerning the use of TREC determination (92.8% and 93.4% respectively) and KREC (85.7% and 96.7% respectively) in patients with ataxia-telangioectasia allow the use of this analysis in laboratory practice. Conclusion. The method of quantitative determination of TREC and KREC due to high values of sensitivity and specificity of the applied technique can be used in the diagnosis of ataxia-teleangiectasia.
{"title":"Quantitative determination of circular DNA fragments of the T- and B-cell receptor TREC/KREC in patients with ataxia-telangiectasia with a mutation in the ATM gene","authors":"E. Polyakova, I. Guryanova, M. Stegantseva, S. Sharapova, I. Sakovich, A. Kupchinskaya, S. Aleshkevich, Y. Zharankova, V. Kazak, T. Volodashchik, M. Belevtsev","doi":"10.14427/jipai.2022.2.6","DOIUrl":"https://doi.org/10.14427/jipai.2022.2.6","url":null,"abstract":"Purpose. Evaluate the diagnostic significance of quantitative determination of T- and B-cell receptor DNA ring fragments in peripheral blood of patients for diagnosis of ataxia-teleangiectasia. Materials and methods. The subjects of the study were peripheral blood samples from 14 patients diagnosed with ataxia-teleangiectasia aged 4.9 (1.6; 14.4) years with mutations in the ATM gene and from 40 healthy children aged 11.0 (6.7; 14.8) years. Results and discussion. Patients with ataxia-teleangiectasia revealed a significant decrease in TREC and KREC values (p<0.05) compared to the control group of healthy children. Diagnostic sensitivity and specificity concerning the use of TREC determination (92.8% and 93.4% respectively) and KREC (85.7% and 96.7% respectively) in patients with ataxia-telangioectasia allow the use of this analysis in laboratory practice. Conclusion. The method of quantitative determination of TREC and KREC due to high values of sensitivity and specificity of the applied technique can be used in the diagnosis of ataxia-teleangiectasia.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124249206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.14427/jipai.2022.1.53
R. Ovchinnikov, E. Bratchikova, N. Gareeva, A. Gaynullina, V. Savinov, L. Said-Uelina, A. Cherednikova, E. Guryanova, A. Kapustin
Fungal culture represents the «gold standard» for diagnosing infections caused by dermatophytes. However, laboratories and trained mycological staff are required for culture diagnostics. It is desirable to introduce methods for point-of-care diagnosis of dermatophytosis, which would be effective and easy to use. The aim of the study was the approbation of the domestic diagnostic media DTM-Expert for the diagnosis of human dermatophytosis in the conditions of dermatovenerological dispensaries. In two separated healthcare dispensaries, a cultural study of 40 clinical samples was conducted using DTM-Expert medium in parallel with Sabouraud medium. It was established that the isolation rate of dermatophytes on the DTM-Expert medium was 42.5%, while on the Sabouraud medium it was only 27.5%. Twelve isolates of Microsporum canis, 2 of Trichophyton rubrum, 3 of Microsporum spp. were isolated at DTM-Expert. Eleven isolates of M. canis were obtained on Sabouraud's medium, while T. rubrum was not isolated at all. Dermatophyte M. canis caused reddening of the DTM-Expert medium at 6.6 days on average, thus providing a quick visual indication of pathogens. Contamination on the experimental medium was observed in 5% of cases, and in 42.5% on the Sabouraud medium. The data obtained allow us to recommend the DTM-Expert diagnostic media for implementation in medical healthcare practice.
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