Pub Date : 2022-01-01DOI: 10.14427/jipai.2022.1.17
V. Okulich, A. Pinchuk, V. Shilin, N. Kolchanova, E. Matusevich, T. Lepteeva, A. Korzhova
Modern diagnostic kits and equipment for species determination and testing of antibiotic resistance of many bacteria have been developed by the medical industry in different countries. However, the range of antibacterial drugs used often does not match those certified in EurAsEC and does not take into account the ability of microorganisms to form biofilm. The purpose of this research was to create diagnostic kits for species identification and testing of antibiotic resistance of Streptococcus spp. pathogens with the ability to make a biofilm matrix. The outcome of the study was the development and registration of diagnostic kits AB-STRВ and ID-STREP, which allow determination of sensitivity to 19 antibacterial drugs most commonly used in medical institutions, and identification 44 types of bacteria in the family Streptococcaceae, as well as the ability of bacteria to form biofilm.
{"title":"ID-STREP и AB-STRВ diagnostic kits for diagnosing streptococcus infections and determination of antibiotic resistance including propensity to biofilm formation","authors":"V. Okulich, A. Pinchuk, V. Shilin, N. Kolchanova, E. Matusevich, T. Lepteeva, A. Korzhova","doi":"10.14427/jipai.2022.1.17","DOIUrl":"https://doi.org/10.14427/jipai.2022.1.17","url":null,"abstract":"Modern diagnostic kits and equipment for species determination and testing of antibiotic resistance of many bacteria have been developed by the medical industry in different countries. However, the range of antibacterial drugs used often does not match those certified in EurAsEC and does not take into account the ability of microorganisms to form biofilm. The purpose of this research was to create diagnostic kits for species identification and testing of antibiotic resistance of Streptococcus spp. pathogens with the ability to make a biofilm matrix. The outcome of the study was the development and registration of diagnostic kits AB-STRВ and ID-STREP, which allow determination of sensitivity to 19 antibacterial drugs most commonly used in medical institutions, and identification 44 types of bacteria in the family Streptococcaceae, as well as the ability of bacteria to form biofilm.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115454979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14427/jipai.2021.4.91
V. Tereshchenko
This paper presents a comparison of cultural and rapid urease tests for the detection of H.pylori presence with PCR in biopsy specimens from patients diagnosed with gastroduodenitis, chronic gastritis, duodenitis and reflux oesophagitis. In general, there was a trend towards more false positive results when using the rapid urease testing method and false negatives when using the cultural method is used, but this pattern was statistically significant only for the diagnoses of gastroduodenitis (for all methods) and chronic gastritis (there was no statistically significant difference between PCR and the cultural method). A prognostic model was constructed to determine the effect of the test method on the outcome.
{"title":"Comparison of methods for the diagnosis of H.pylori in gastric biopsy specimens from patients with different gastrointestinal diseases","authors":"V. Tereshchenko","doi":"10.14427/jipai.2021.4.91","DOIUrl":"https://doi.org/10.14427/jipai.2021.4.91","url":null,"abstract":"This paper presents a comparison of cultural and rapid urease tests for the detection of H.pylori presence with PCR in biopsy specimens from patients diagnosed with gastroduodenitis, chronic gastritis, duodenitis and reflux oesophagitis. In general, there was a trend towards more false positive results when using the rapid urease testing method and false negatives when using the cultural method is used, but this pattern was statistically significant only for the diagnoses of gastroduodenitis (for all methods) and chronic gastritis (there was no statistically significant difference between PCR and the cultural method). A prognostic model was constructed to determine the effect of the test method on the outcome.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129932193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14427/jipai.2021.4.83
D. Tereshko, M. Parfenova
Endemic infections, such as histoplasmosis, the causative agent of which is assigned to pathogenicity group II, can cause a number of difficulties in laboratory studies. The culture method is the gold standard of diagnosis, but requires a lot of time and skill of the doctor. Immunological methods used to detect H. capsulatum antigens and/or antibodies to them, although they have received a wide range of applications in endemic regions show variable sensitivity and specificity, which is influenced by: the nature of the course of infection, the immune status of the patient, the type of clinical material and etc. As a result, we are seeing a gradual shift in emphasis towards molecular genetic studies, which demonstrate high analytical performance in the laboratory diagnosis of histoplasmosis.
{"title":"Methods of laboratory diagnosis of histoplasmosis – problems and prospects","authors":"D. Tereshko, M. Parfenova","doi":"10.14427/jipai.2021.4.83","DOIUrl":"https://doi.org/10.14427/jipai.2021.4.83","url":null,"abstract":"Endemic infections, such as histoplasmosis, the causative agent of which is assigned to pathogenicity group II, can cause a number of difficulties in laboratory studies. The culture method is the gold standard of diagnosis, but requires a lot of time and skill of the doctor. Immunological methods used to detect H. capsulatum antigens and/or antibodies to them, although they have received a wide range of applications in endemic regions show variable sensitivity and specificity, which is influenced by: the nature of the course of infection, the immune status of the patient, the type of clinical material and etc. As a result, we are seeing a gradual shift in emphasis towards molecular genetic studies, which demonstrate high analytical performance in the laboratory diagnosis of histoplasmosis.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127987629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14427/jipai.2021.4.49
O. Kutsevalova, A. Antonets, V. Krylov, N. Nifantiev, D. Miroshnichenko, A. Mikutin
The COVID-19 pandemic has revealed the new forms of invasive fungal infections. Without timely diagnosis and treatment, mortality reaches 100%. We analyzed 31 observed cases of invasive mucormycosis and aspergillosis in patients with COVID-19 to identify risk factors, the time sequence of the onset of clinical symptoms, and diagnostic features. Major predisposing factors were diabetes mellitus, immunosuppressive therapy, and oncological diseases.
{"title":"COVID-19 associated invasive mycoses","authors":"O. Kutsevalova, A. Antonets, V. Krylov, N. Nifantiev, D. Miroshnichenko, A. Mikutin","doi":"10.14427/jipai.2021.4.49","DOIUrl":"https://doi.org/10.14427/jipai.2021.4.49","url":null,"abstract":"The COVID-19 pandemic has revealed the new forms of invasive fungal infections. Without timely diagnosis and treatment, mortality reaches 100%. We analyzed 31 observed cases of invasive mucormycosis and aspergillosis in patients with COVID-19 to identify risk factors, the time sequence of the onset of clinical symptoms, and diagnostic features. Major predisposing factors were diabetes mellitus, immunosuppressive therapy, and oncological diseases.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124148154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14427/jipai.2021.4.60
O. Enoktaeva, M. Nikolenko, D. Trushnikov, N. Baryshnikova
The study of the chemical composition of the cell wall of fungi of the genus Candida sp. is an actual trend in modern medicine, allowing the development of new methods for the treatment of candidiasis. The polysaccharides mannan, glucan, and chitin form the framework of the cell wall, inside and on the surface of which proteins and other molecules are present. The components of the cell wall perform a wide range of functions: protect the contents of the cell from external factors; provide nutrition, respiration, reproduction and formation of cells of various morphologies; mediate the pathogenic properties of fungi, including biofilm formation, spread, and the ability to modulate the host's immune response.
{"title":"The cell wall as a mediator of the pathogenic activity of fungi of the genus Candida sp.","authors":"O. Enoktaeva, M. Nikolenko, D. Trushnikov, N. Baryshnikova","doi":"10.14427/jipai.2021.4.60","DOIUrl":"https://doi.org/10.14427/jipai.2021.4.60","url":null,"abstract":"The study of the chemical composition of the cell wall of fungi of the genus Candida sp. is an actual trend in modern medicine, allowing the development of new methods for the treatment of candidiasis. The polysaccharides mannan, glucan, and chitin form the framework of the cell wall, inside and on the surface of which proteins and other molecules are present. The components of the cell wall perform a wide range of functions: protect the contents of the cell from external factors; provide nutrition, respiration, reproduction and formation of cells of various morphologies; mediate the pathogenic properties of fungi, including biofilm formation, spread, and the ability to modulate the host's immune response.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"136 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127356335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14427/jipai.2021.4.77
G. Kononenko
The review presents an analysis of the current state of the problem associated with the production of toxic metabolites by three species of fungi of the genus Aspergillus – A. flavus, A. niger and A. fumigatus. All of them are known for their extensive occurrence in the environment, pathogenicity and traditional use in industrial biotechnology. In recent decades aflatoxins, cyclopiazonic acid, fumonisins of group B, gliotoxin and clavine ergot alkaloids have been assigned to the group of priority attention from the whole variety of structural types of toxins peculiar to these fungi. Large-scale studies of A. flavus and A. niger populations infecting agricultural products have been carried out in many regions of the world, their chemo- and phenotypic composition has been described, the prospects of molecular methods for improving the safety of industrial cultures and replacement strains recommended for biological correction have been shown, and new information has been obtained confirming the participation of toxic metabolites of A. fumigatus in the development of pathogenesis.
{"title":"Toxigenic cosmopolitan micromycetes of the genus Aspergillus: new facts of recent decades","authors":"G. Kononenko","doi":"10.14427/jipai.2021.4.77","DOIUrl":"https://doi.org/10.14427/jipai.2021.4.77","url":null,"abstract":"The review presents an analysis of the current state of the problem associated with the production of toxic metabolites by three species of fungi of the genus Aspergillus – A. flavus, A. niger and A. fumigatus. All of them are known for their extensive occurrence in the environment, pathogenicity and traditional use in industrial biotechnology. In recent decades aflatoxins, cyclopiazonic acid, fumonisins of group B, gliotoxin and clavine ergot alkaloids have been assigned to the group of priority attention from the whole variety of structural types of toxins peculiar to these fungi. Large-scale studies of A. flavus and A. niger populations infecting agricultural products have been carried out in many regions of the world, their chemo- and phenotypic composition has been described, the prospects of molecular methods for improving the safety of industrial cultures and replacement strains recommended for biological correction have been shown, and new information has been obtained confirming the participation of toxic metabolites of A. fumigatus in the development of pathogenesis.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"157 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128782470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14427/jipai.2021.4.65
A. Lipnitsky, N. Polovets, R. Surkova, A. Murugova, A. Toporkov, D. Victorov
Mucormycosis is an angioinvasive disease caused by fungi of the order Mucorales. Coronavirus disease (COVID-19), associated mucormycosis (CAM) is a global emerging threat. More than 40 000 CAM cases have been reported in India. Emergense of CAM has been attributed to host, environmental and agents-related factors. Mucorales spore burden has been reported globally, however their presence is higher in tropical countries, such as India. Before the COVID-19 pandemic, patients with diabetes mellitus, hematological malignancies, solid organ transplants, corticosteroid therapy and neutropenia were more prone to mucormycosis. In COVID-19 patients, virus-induced endothelial dysfunction, hyperglycemia, and immune dysfunction following corticosteroid use. COVID-19 infection may directly induce hyperglycaemia by damaging beta cells of the pancreas or by corticosteroid therapy. In addition, corticosteroid treatment reduces the innate immune function of phagocytic cells. Free iron acquisition from the host is an important virulence factor of Mucorales.
{"title":"COVID-19 and mucormycosis","authors":"A. Lipnitsky, N. Polovets, R. Surkova, A. Murugova, A. Toporkov, D. Victorov","doi":"10.14427/jipai.2021.4.65","DOIUrl":"https://doi.org/10.14427/jipai.2021.4.65","url":null,"abstract":"Mucormycosis is an angioinvasive disease caused by fungi of the order Mucorales. Coronavirus disease (COVID-19), associated mucormycosis (CAM) is a global emerging threat. More than 40 000 CAM cases have been reported in India. Emergense of CAM has been attributed to host, environmental and agents-related factors. Mucorales spore burden has been reported globally, however their presence is higher in tropical countries, such as India. Before the COVID-19 pandemic, patients with diabetes mellitus, hematological malignancies, solid organ transplants, corticosteroid therapy and neutropenia were more prone to mucormycosis. In COVID-19 patients, virus-induced endothelial dysfunction, hyperglycemia, and immune dysfunction following corticosteroid use. COVID-19 infection may directly induce hyperglycaemia by damaging beta cells of the pancreas or by corticosteroid therapy. In addition, corticosteroid treatment reduces the innate immune function of phagocytic cells. Free iron acquisition from the host is an important virulence factor of Mucorales.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127744291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14427/jipai.2021.4.72
I. Khamaganova
The significant growth and spread of dermatophytosis caused by Microsporum canis determine the interest to the research of this causative agent. The last time such technologies as metabolomics,transcriptomics and proteomics appeared to be effective. The role of zinc was examined. Zinc deficiency effect on gene expression, metabolism and pathogenicity of Microsporum canis. It was also proved that cyclic lipopeptides obtained from Bacillus subtilis inhibit the growth of cat’s Microsporum canis.
{"title":"Some prospects in the reseach of Microsporum canis (review)","authors":"I. Khamaganova","doi":"10.14427/jipai.2021.4.72","DOIUrl":"https://doi.org/10.14427/jipai.2021.4.72","url":null,"abstract":"The significant growth and spread of dermatophytosis caused by Microsporum canis determine the interest to the research of this causative agent. The last time such technologies as metabolomics,transcriptomics and proteomics appeared to be effective. The role of zinc was examined. Zinc deficiency effect on gene expression, metabolism and pathogenicity of Microsporum canis. It was also proved that cyclic lipopeptides obtained from Bacillus subtilis inhibit the growth of cat’s Microsporum canis.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129402094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14427/jipai.2021.4.42
R. Ovchinnikov, N. Polyakov, A. Soloviev, A. Gainullina, V. Savinov, A. Kapustin, A. Gulyukin
Microsporum canis is an important fungal pathogen that is mainly transmitted to humans from domestic animals. Different strains of M. canis vary in their ability to infect humans. Comparative studies of M. canis strains from humans and animals using MALDI mass spectrometry have not yet been published. In a comparative aspect, M. canis strains isolated from human (HS 09-18) and cat (FC 19-18) were studied. Comparison of the obtained mass spectra revealed that M. canis HS 09-18 displayed characteristic peaks with m/z 3339, 4287, 6485, 7740 and 8568, while M. canis FC 19-18 displayed unique peaks with m/z 5192, 7221 and 8049. Three peaks with maximum intensity with m/z 6112, 6828, 9625 were found in both M. canis strains. The dendrogram built on the basis of MALDI mass spectra revealed the clustering of M. canis strains in accordance with their origin: strains from cats and humans formed two separate clusters. Thus, clear differences were demonstrated between M. canis strains depending on the source of origin (cat and human). MALDI mass spectrometry makes it possible to carry out species identification, as well as to reveal intraspecific differences in M. canis. The mechanisms that allow dermatophytes to switch from zoophilic to anthropophilic behavior need to be further explored.
犬小孢子菌是一种主要由家畜传播给人类的重要真菌病原体。不同种类的犬支原体感染人类的能力各不相同。使用MALDI质谱法对人类和动物的犬支原体菌株进行比较研究尚未发表。在比较方面，研究了人(HS 09-18)和猫(FC 19-18)分离的犬支原体菌株。质谱分析结果表明，犬m.s HS 09-18的特征峰为m/z 3339、4287、6485、7740和8568，而犬m.s FC 19-18的特征峰为m/z 5192、7221和8049。两株犬支原体均有3个最大强度峰，分别为m/z 6112、6828、9625。在MALDI质谱的基础上建立的树状图显示，犬支原体菌株按照其来源聚类:来自猫和人类的菌株形成两个独立的聚类。因此，根据来源(猫和人)不同，犬支原体菌株之间存在明显差异。MALDI质谱分析使得进行物种鉴定以及揭示犬支原体种内差异成为可能。允许皮肤植物从嗜兽行为转变为嗜人行为的机制需要进一步探索。
{"title":"Application of MALDI mass spectrometry for species identification and intraspecific differentiation of dermatophyte Microsporum canis of anthroponotic and zoonotic origin","authors":"R. Ovchinnikov, N. Polyakov, A. Soloviev, A. Gainullina, V. Savinov, A. Kapustin, A. Gulyukin","doi":"10.14427/jipai.2021.4.42","DOIUrl":"https://doi.org/10.14427/jipai.2021.4.42","url":null,"abstract":"Microsporum canis is an important fungal pathogen that is mainly transmitted to humans from domestic animals. Different strains of M. canis vary in their ability to infect humans. Comparative studies of M. canis strains from humans and animals using MALDI mass spectrometry have not yet been published. In a comparative aspect, M. canis strains isolated from human (HS 09-18) and cat (FC 19-18) were studied. Comparison of the obtained mass spectra revealed that M. canis HS 09-18 displayed characteristic peaks with m/z 3339, 4287, 6485, 7740 and 8568, while M. canis FC 19-18 displayed unique peaks with m/z 5192, 7221 and 8049. Three peaks with maximum intensity with m/z 6112, 6828, 9625 were found in both M. canis strains. The dendrogram built on the basis of MALDI mass spectra revealed the clustering of M. canis strains in accordance with their origin: strains from cats and humans formed two separate clusters. Thus, clear differences were demonstrated between M. canis strains depending on the source of origin (cat and human). MALDI mass spectrometry makes it possible to carry out species identification, as well as to reveal intraspecific differences in M. canis. The mechanisms that allow dermatophytes to switch from zoophilic to anthropophilic behavior need to be further explored.","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122895237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.14427/jipai.2021.4.14
O. Korotina, I. Generalov
Catalytic abzyme activity of IgA and corresponding enzymes of oral fluid in patients with chronic periodontitis was studied. The individuals with healthy state of oral cavity were used as controls. Various kinds of proteolytic, oxidoreductase, and DNase activity were investigated; the concentarion of oral fluid DNA was evaluated as well. IgA fractions were isolated from the oral fluids by affinity chromatography on a matrix with polyclonal antibodies against human IgA, Elastase, proteolytic, cathepsin-like and amidase activities were investigated by colorimetric methods. DNase activity was assessed by the rivanol clot test. DNA concentrations in the oral fluid were determined by fluorimetric DNA test with PicoGreen fluorochrome. It has been established that certain kinds of IgA abzyme activities and oral fluid enzymes become substantially elevated following the exacerbations of chronic periodontitis in comparison with healthy controls (p<0.01-0.001). It refers to elastase, peroxidase, cathepsin, and catalase activity of oral cavity. The progression of chronic periodontitis is also characterized by high levels of patients’ DNA contents in oral fluid (p<0.001). For the diagnosis of chronic periodontitis the ROC-analysis of examined laboratory markers found the maximum diagnostic value of oral elastase testing (specificity of the test – 0,97; sensitivity – 0,85).
{"title":"Secretory abzyme IgA, enzymes and DNA of oral fluid in chronic periodontitis","authors":"O. Korotina, I. Generalov","doi":"10.14427/jipai.2021.4.14","DOIUrl":"https://doi.org/10.14427/jipai.2021.4.14","url":null,"abstract":"Catalytic abzyme activity of IgA and corresponding enzymes of oral fluid in patients with chronic periodontitis was studied. The individuals with healthy state of oral cavity were used as controls. Various kinds of proteolytic, oxidoreductase, and DNase activity were investigated; the concentarion of oral fluid DNA was evaluated as well. IgA fractions were isolated from the oral fluids by affinity chromatography on a matrix with polyclonal antibodies against human IgA, Elastase, proteolytic, cathepsin-like and amidase activities were investigated by colorimetric methods. DNase activity was assessed by the rivanol clot test. DNA concentrations in the oral fluid were determined by fluorimetric DNA test with PicoGreen fluorochrome. It has been established that certain kinds of IgA abzyme activities and oral fluid enzymes become substantially elevated following the exacerbations of chronic periodontitis in comparison with healthy controls (p<0.01-0.001). It refers to elastase, peroxidase, cathepsin, and catalase activity of oral cavity. The progression of chronic periodontitis is also characterized by high levels of patients’ DNA contents in oral fluid (p<0.001). For the diagnosis of chronic periodontitis the ROC-analysis of examined laboratory markers found the maximum diagnostic value of oral elastase testing (specificity of the test – 0,97; sensitivity – 0,85).","PeriodicalId":425120,"journal":{"name":"Immunopathology, Allergology, Infectology","volume":"206 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124632739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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