Zaid Q. Abd Al-Razaq, Mahmood Shakir Khudhair, Khalid Abdulhussein Sabry
Objectives: Primary aldosteronism is a prevalent contributor to secondary hypertension, posing an elevated risk of morbidity and mortality. The initial step in diagnosing this condition involves screening individuals suspected of having it.
Methods: In our study, we enrolled 50 patients who were suspected of having hyperaldosteronism. We provided specific recommendations and instructions to these patients concerning drug therapy and implemented measures to enhance testing accuracy. The tests were conducted using the Chemiluminescence Immunoassay (CLIA) system procedure, relying on plasma direct renin concentration rather than activity. Our findings were meticulously validated, compared, and aligned with the references from ARUP laboratories.
Results: Among our participants, 4 patients (8%) were unequivocally diagnosed with primary aldosteronism based on the Aldosterone Direct Renin Concentration (ADRR) criteria. These patients exhibited the trifecta of hypokalemia, elevated aldosterone levels, and diminished renin levels, obviating the need for further confirmatory testing. Furthermore, 13 patients (26%) were deemed likely candidates for primary aldosteronism, given their plasma aldosterone levels exceeding 15 ng/dl and renin levels below 2.5 pg/ml. Additionally, 7 patients (14%) displayed strong indications of primary aldosteronism, characterized by plasma aldosterone levels surpassing 21 ng/dl and plasma renin concentrations below 2.5 pg/ml, accompanied by high ADRR values. However, both the "likely" and "strong indication" groups necessitated confirmatory testing. Notably, our results revealed no significant disparities in age, gender, personal or family history of atherosclerotic cardiovascular disease (ASCVD), or the presence of adrenal adenomas between patients diagnosed with primary aldosteronism and those in the non-aldosteronism group within the study.
Conclusion: Primary aldosteronism is a prevalent health concern, warranting the screening of highly suspicious patients. Utilizing direct renin concentration, instead of renin activity, offers a straightforward, cost-effective, rapid, and reproducible method for diagnosis.
{"title":"Diagnostic Utility of Aldosterone/Direct Renin Concentration Ratio in Iraqi Patients with High Arterial Blood Pressure: A Pilot Study","authors":"Zaid Q. Abd Al-Razaq, Mahmood Shakir Khudhair, Khalid Abdulhussein Sabry","doi":"10.22317/jcms.v9i4.1398","DOIUrl":"https://doi.org/10.22317/jcms.v9i4.1398","url":null,"abstract":"
 
 
 Objectives: Primary aldosteronism is a prevalent contributor to secondary hypertension, posing an elevated risk of morbidity and mortality. The initial step in diagnosing this condition involves screening individuals suspected of having it.
 Methods: In our study, we enrolled 50 patients who were suspected of having hyperaldosteronism. We provided specific recommendations and instructions to these patients concerning drug therapy and implemented measures to enhance testing accuracy. The tests were conducted using the Chemiluminescence Immunoassay (CLIA) system procedure, relying on plasma direct renin concentration rather than activity. Our findings were meticulously validated, compared, and aligned with the references from ARUP laboratories.
 Results: Among our participants, 4 patients (8%) were unequivocally diagnosed with primary aldosteronism based on the Aldosterone Direct Renin Concentration (ADRR) criteria. These patients exhibited the trifecta of hypokalemia, elevated aldosterone levels, and diminished renin levels, obviating the need for further confirmatory testing. Furthermore, 13 patients (26%) were deemed likely candidates for primary aldosteronism, given their plasma aldosterone levels exceeding 15 ng/dl and renin levels below 2.5 pg/ml. Additionally, 7 patients (14%) displayed strong indications of primary aldosteronism, characterized by plasma aldosterone levels surpassing 21 ng/dl and plasma renin concentrations below 2.5 pg/ml, accompanied by high ADRR values. However, both the \"likely\" and \"strong indication\" groups necessitated confirmatory testing. Notably, our results revealed no significant disparities in age, gender, personal or family history of atherosclerotic cardiovascular disease (ASCVD), or the presence of adrenal adenomas between patients diagnosed with primary aldosteronism and those in the non-aldosteronism group within the study.
 Conclusion: Primary aldosteronism is a prevalent health concern, warranting the screening of highly suspicious patients. Utilizing direct renin concentration, instead of renin activity, offers a straightforward, cost-effective, rapid, and reproducible method for diagnosis.
 
 
","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135236083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Toll-like receptor 7 (TLR7) and MyD88 represent important components of the innate immune response which play a crucial role in recognition of T. vaginalis. Single nucleotide polymorphisms (SNPs) in TLRs and MyD88 were manifested as key determinant affecting the susceptibility to trichomoniasis. This study aims to examine the impact of two SNPs, designated rs179008 and rs4988453 in TLR7 and MyD88, respectively, on Iraqi women infected with T. vaginalis.
Methods: Women vaginal swabs as well as blood samples were collected from 186 female patients diagnosed clinically by gynecologists. These pateints were admitting the gynecology clinics in three public hospitals in Babel governorate in Iraq. Clinical samples were obtained for molecular identification of the parasite, sequencing of the TLR7 and MyD88 genes as well as performing the corresponding immunological studies.
Results: The PCR assays showed 40 positive women (95% CI, 15.85 to 28.11) of T. vaginalis β-tubulin gene. Genetic studies of rs179008 SNP in TLR7 showed that the mutant T allele revealed significantly more prevalence in women infected with T. vaginalis in comparison with controls (p<0.001), furthermore, AT and TT genotypes distribution were significantly greater in patients than that in controls (p<0.01). Moreover, genetic analysis of rs4988453 SNP in MyD88 demonstrated that the mutant A allele almost has close frequency between patients and controls, and the heterozygous CA and homozygous AA genotypes were almost normally distributed between controls and patients. Finally, the concentrations of TLR7 and MyD88 were significantly elevated in the majority of women patients aged between 16-40 years.
Conclusion: The mutant allele A of rs4988453 SNP in MyD88 did not show an association with increased risk of trichomonas infection, however, the mutant allele T of rs179008 SNP in TLR7 might make women more sensitive for infection with T. vaginalis. However, more studies are needed to confirm these findings and to understand the underlying involved mechanisms.
{"title":"Association of TLR7 and MyD88 Gene Polymorphism with Trichomoniasis vaginalis Infection","authors":"Zainab Waddah Kermasha, Hayam Khalis Al-Masoudi, Suhaila Fadhil Al-Shaikh","doi":"10.22317/jcms.v9i4.1390","DOIUrl":"https://doi.org/10.22317/jcms.v9i4.1390","url":null,"abstract":"Objective: Toll-like receptor 7 (TLR7) and MyD88 represent important components of the innate immune response which play a crucial role in recognition of T. vaginalis. Single nucleotide polymorphisms (SNPs) in TLRs and MyD88 were manifested as key determinant affecting the susceptibility to trichomoniasis. This study aims to examine the impact of two SNPs, designated rs179008 and rs4988453 in TLR7 and MyD88, respectively, on Iraqi women infected with T. vaginalis.
 Methods: Women vaginal swabs as well as blood samples were collected from 186 female patients diagnosed clinically by gynecologists. These pateints were admitting the gynecology clinics in three public hospitals in Babel governorate in Iraq. Clinical samples were obtained for molecular identification of the parasite, sequencing of the TLR7 and MyD88 genes as well as performing the corresponding immunological studies.
 Results: The PCR assays showed 40 positive women (95% CI, 15.85 to 28.11) of T. vaginalis β-tubulin gene. Genetic studies of rs179008 SNP in TLR7 showed that the mutant T allele revealed significantly more prevalence in women infected with T. vaginalis in comparison with controls (p<0.001), furthermore, AT and TT genotypes distribution were significantly greater in patients than that in controls (p<0.01). Moreover, genetic analysis of rs4988453 SNP in MyD88 demonstrated that the mutant A allele almost has close frequency between patients and controls, and the heterozygous CA and homozygous AA genotypes were almost normally distributed between controls and patients. Finally, the concentrations of TLR7 and MyD88 were significantly elevated in the majority of women patients aged between 16-40 years.
 Conclusion: The mutant allele A of rs4988453 SNP in MyD88 did not show an association with increased risk of trichomonas infection, however, the mutant allele T of rs179008 SNP in TLR7 might make women more sensitive for infection with T. vaginalis. However, more studies are needed to confirm these findings and to understand the underlying involved mechanisms.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135236406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samar Jassim Ibrahim, Lelas Farhan Bdaiwi, Muthanna Asaad Al Sherbit, Yasser Fawzi Zidan
Objectives: This study aimed to assess the impact of bariatric surgery on thyrotropin (TSH), calcium, vitamin D, and lipid profile levels three months post-surgery among patients who underwent the procedure. We also investigated the prevalence of micronutrient deficiencies during the initial three months before and after surgery, along with monitoring the effects of gastric sleeve surgery on body mass index (BMI) reduction.
Methods: The study involved the analysis of data from a cohort comprising 30 patients of both genders who had undergone sleeve gastrectomy due to obesity, with a preoperative BMI of 40 kg/m² or higher. The research spanned from July 2022 to January 2023, with patients undergoing gastric sleeve surgery at Al-Zahrawi Hospital in Nineveh. These patients were subsequently followed up for a period of three months post-surgery.
Results: The study findings demonstrated a significant reduction in TSH, vitamin D, calcium, total cholesterol (TC), triglycerides (TG), low-density lipoproteins (LDLc), very low-density lipoproteins (VLDLc) cholesterol, and BMI in patients who underwent gastric sleeve surgery within the three-month follow-up period, in comparison to their pre-surgery levels. Additionally, there was a noteworthy increase in high-density lipoproteins of cholesterol (HDLc) in the serum of these patients post-operation. The results further indicated positive correlations between TSH, vitamin D, cholesterol, LDLc, and the Atherogenic Index. Likewise, positive correlations were observed between vitamin D, TSH, total cholesterol, triglycerides (TG), and LDLc.
Conclusion: Post-surgery deficiencies in vitamin D, calcium, and TSH levels are attributed to inadequate intake and absorption of micronutrients, highlighting the importance of nutritional supplementation. Sleeve gastrectomy offers a promising avenue for sustained BMI reduction in the long term.
{"title":"Effects of Sleeve Gastrectomy on Thyrotropin Hormone (TSH) and Vitamin D Levels among Obese Patients in Nineveh Governorate","authors":"Samar Jassim Ibrahim, Lelas Farhan Bdaiwi, Muthanna Asaad Al Sherbit, Yasser Fawzi Zidan","doi":"10.22317/jcms.v9i4.1408","DOIUrl":"https://doi.org/10.22317/jcms.v9i4.1408","url":null,"abstract":"Objectives: This study aimed to assess the impact of bariatric surgery on thyrotropin (TSH), calcium, vitamin D, and lipid profile levels three months post-surgery among patients who underwent the procedure. We also investigated the prevalence of micronutrient deficiencies during the initial three months before and after surgery, along with monitoring the effects of gastric sleeve surgery on body mass index (BMI) reduction.
 Methods: The study involved the analysis of data from a cohort comprising 30 patients of both genders who had undergone sleeve gastrectomy due to obesity, with a preoperative BMI of 40 kg/m² or higher. The research spanned from July 2022 to January 2023, with patients undergoing gastric sleeve surgery at Al-Zahrawi Hospital in Nineveh. These patients were subsequently followed up for a period of three months post-surgery.
 Results: The study findings demonstrated a significant reduction in TSH, vitamin D, calcium, total cholesterol (TC), triglycerides (TG), low-density lipoproteins (LDLc), very low-density lipoproteins (VLDLc) cholesterol, and BMI in patients who underwent gastric sleeve surgery within the three-month follow-up period, in comparison to their pre-surgery levels. Additionally, there was a noteworthy increase in high-density lipoproteins of cholesterol (HDLc) in the serum of these patients post-operation. The results further indicated positive correlations between TSH, vitamin D, cholesterol, LDLc, and the Atherogenic Index. Likewise, positive correlations were observed between vitamin D, TSH, total cholesterol, triglycerides (TG), and LDLc.
 Conclusion: Post-surgery deficiencies in vitamin D, calcium, and TSH levels are attributed to inadequate intake and absorption of micronutrients, highlighting the importance of nutritional supplementation. Sleeve gastrectomy offers a promising avenue for sustained BMI reduction in the long term.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135236375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmed H. Al-Azawi, Riyadh Abd-Alrasool Hneua, R. Hameed, D. Hassan
Objectives: In this trial, It has been investigated whether pertuzumab, when added to adjuvant trastuzumab and chemotherapy, improves outcomes among patients with HER2-positive early breast cancer in compares to patients who received only Herceptin Methods: After surgery and central HER2-positive confirmation, about randomly 220 patients assigned with high-risk HER2-positive, operable breast cancer to received Anthracycline based chemotherapy 3 cycles fallowed by Taxotere 3 + either pertuzumab + hercetin 3 or standard adjuvant Herceptin alone, 17 cycles in 1 year. The patients were followed up for 3 years. Results: Results were indicated that about 50% of the patients who were randomly assigned to arm A received pertuzumab+Herceptin 3 cycles every 3 weeks (110 patients) and arm B 50% (110 patients) received Herceptin alone 17 cycles every 3 weeks. Disease recurrence occurred in 12 patients (10.9 %) in the pertuzumab group and 8 patients (7.2%) in the arm B group (hazard ratio, 0.81; 95% confidence interval [CI], 0.66 to 1.00; P=0.045). The estimates of the 3-year rates of invasive-disease-free survival were 89% in the pertuzumab+ herceptin group and 93% in the herceptin group. Heart failure, cardiac death, and cardiac dysfunction were infrequent in both treatment groups. Diarrhea of grade 3 or higher occurred almost exclusively during chemotherapy and it was more frequent with pertuzumab than with group B (7.9% vs. 2.8%). Conclusions: The study showed that pertuzumab + Trastuzumab adjuvant in 3 cycles worse rates of invasive-disease-free survival among patients with HER2-positive, operable breast cancer in compares with classical trastuzumab alone in 17 cycles. Diarrhea was more common with pertuzumab than with classical Trastuzumab therapy.
{"title":"The Effect of Adding Pertuzumab to Adjuvant Trastuzumab in Early HER2-Positive Breast Cancer","authors":"Ahmed H. Al-Azawi, Riyadh Abd-Alrasool Hneua, R. Hameed, D. Hassan","doi":"10.22317/jcms.v9i3.1351","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1351","url":null,"abstract":"Objectives: In this trial, It has been investigated whether pertuzumab, when added to adjuvant trastuzumab and chemotherapy, improves outcomes among patients with HER2-positive early breast cancer in compares to patients who received only Herceptin \u0000Methods: After surgery and central HER2-positive confirmation, about randomly 220 patients assigned with high-risk HER2-positive, operable breast cancer to received Anthracycline based chemotherapy 3 cycles fallowed by Taxotere 3 + either pertuzumab + hercetin 3 or standard adjuvant Herceptin alone, 17 cycles in 1 year. The patients were followed up for 3 years. \u0000Results: Results were indicated that about 50% of the patients who were randomly assigned to arm A received pertuzumab+Herceptin 3 cycles every 3 weeks (110 patients) and arm B 50% (110 patients) received Herceptin alone 17 cycles every 3 weeks. Disease recurrence occurred in 12 patients (10.9 %) in the pertuzumab group and 8 patients (7.2%) in the arm B group (hazard ratio, 0.81; 95% confidence interval [CI], 0.66 to 1.00; P=0.045). The estimates of the 3-year rates of invasive-disease-free survival were 89% in the pertuzumab+ herceptin group and 93% in the herceptin group. Heart failure, cardiac death, and cardiac dysfunction were infrequent in both treatment groups. Diarrhea of grade 3 or higher occurred almost exclusively during chemotherapy and it was more frequent with pertuzumab than with group B (7.9% vs. 2.8%). \u0000Conclusions: The study showed that pertuzumab + Trastuzumab adjuvant in 3 cycles worse rates of invasive-disease-free survival among patients with HER2-positive, operable breast cancer in compares with classical trastuzumab alone in 17 cycles. Diarrhea was more common with pertuzumab than with classical Trastuzumab therapy. ","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48602104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives: Serratia marcescens is a gram-negative pathogen of many species. The ability of S. marcescens to form biofilms and its potent innate resistance to antimicrobials and cleaning solutions are both essential for its pathogenicity and survival. The present study was conducted to investigate the effect of glyceryl trinitrate (GTN) on the biofilm of S. marcescens, as an alternative for antibiotic therapy. Methods: Different specimens, including ear swabs, burns, mid-stream urine, wound swabs, and sputum, were collected from patients who were brought to Al-Ramadi Hospital, Iraq. All samples were cultured, and the colonies that were obtained were detected using the VITEK® 2 compact. The ability of biofilms to develop was examined using the microtiter plate technique. The bactericidal effectiveness of GTN was estimated by the broth microdilution technique. The presence of fimA and fimC in S. marcescens isolates was detected using the polymerase chain reaction (PCR) method. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to assess the effect of GTN on fimA and fimC gene expression. Results: The results demonstrated that GTN has no effect on S. marcescens growth; while its biofilm was significantly (p<0.05) influenced. Moreover, all S. marcescens isolates had fimA and fimC, and the presence of GTN reduced the expression of these genes. Conclusion: The findings of this study reveal that GTN can act as a promising antibiofilm agent in reference to S. marcescens.
{"title":"Glyceryl Trinitrate, a Vasodilating Drug Acts as an Antibiofilm Agent in Serratia marcescens","authors":"Z. Al-Fayyadh, A. Turki, H. Al-Mathkhury","doi":"10.22317/jcms.v9i3.1329","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1329","url":null,"abstract":"Objectives: Serratia marcescens is a gram-negative pathogen of many species. The ability of S. marcescens to form biofilms and its potent innate resistance to antimicrobials and cleaning solutions are both essential for its pathogenicity and survival. The present study was conducted to investigate the effect of glyceryl trinitrate (GTN) on the biofilm of S. marcescens, as an alternative for antibiotic therapy. \u0000Methods: Different specimens, including ear swabs, burns, mid-stream urine, wound swabs, and sputum, were collected from patients who were brought to Al-Ramadi Hospital, Iraq. All samples were cultured, and the colonies that were obtained were detected using the VITEK® 2 compact. The ability of biofilms to develop was examined using the microtiter plate technique. The bactericidal effectiveness of GTN was estimated by the broth microdilution technique. The presence of fimA and fimC in S. marcescens isolates was detected using the polymerase chain reaction (PCR) method. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to assess the effect of GTN on fimA and fimC gene expression. \u0000Results: The results demonstrated that GTN has no effect on S. marcescens growth; while its biofilm was significantly (p<0.05) influenced. Moreover, all S. marcescens isolates had fimA and fimC, and the presence of GTN reduced the expression of these genes. \u0000Conclusion: The findings of this study reveal that GTN can act as a promising antibiofilm agent in reference to S. marcescens.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41613906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: In this study, we report that a novel combination therapy of different statins with Ibrutinib can overcome such resistance. Methods: For this, we generated a cell line model, exhibiting resistance to Ibrutinib, by repeated exposure of mantle cell lymphoma cell line to Ibrutinib. Apoptosis was assessed by the extent of phosphatidylserine externalisation. Results: Our results indicated that resistance to Ibrutinib could be overcome by targeting a key enzyme, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in the cholesterogenesis pathway. Conclusion: Reusing different statins in combination with Ibrutinib could improve therapy and enhance sensitivity to Ibrutinib mediated apoptosis.
{"title":"Statins in combination with Ibrutinib bypasses resistance to Ibrutinib in mantle cell lymphoma","authors":"A. Al-Zebeeby, A. Abbas","doi":"10.22317/jcms.v9i3.1330","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1330","url":null,"abstract":"Objective: In this study, we report that a novel combination therapy of different statins with Ibrutinib can overcome such resistance.\u0000Methods: For this, we generated a cell line model, exhibiting resistance to Ibrutinib, by repeated exposure of mantle cell lymphoma cell line to Ibrutinib. Apoptosis was assessed by the extent of phosphatidylserine externalisation.\u0000Results: Our results indicated that resistance to Ibrutinib could be overcome by targeting a key enzyme, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in the cholesterogenesis pathway.\u0000Conclusion: Reusing different statins in combination with Ibrutinib could improve therapy and enhance sensitivity to Ibrutinib mediated apoptosis.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46232597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Baraa Abdul-Kareem Mutar, T. A. Allwsh, Ammar Yassin, F. J. Al-Tu’ma, H. S. Taghi
Objective: This study aims to examine the association between the (rs1544410) polymorphism of the VDR gene with the pathogenesis of T2DM in Iraqi obese women. Methods: A case-control study was performed on 50 patients with T2DM and 50 apparently healthy subjects who were admitted to Al-Hussein Teaching Hospital and Al-Hassan Center of Diabetes and Endocrinology unit / Kerbala health directorate – Iraq during (April 2022 – March 2023). The T2DM groups were divided into two groups, 25 obese and 25 non-obese; the control group was divided into 25 obese and 25 non-obese as apparently healthy groups. The ELISA Kit was used to measure serum 25(OH)D3, heat shock protein-70, VDBP, insulin and C-peptide. Also HbA1c% and insulin resistance (HOMA-IR) were evaluated. The vitamin D3 receptor gene (VDR) variant and the SNP (rs1544410) polymorphism was determined using allele specific polymerase chain reaction, 1.5% agarose gel electrophoresis and then visualized by gel photo-documentation system. Results: The result of vitamin D3 variants genotype (rs 1544410) was a clear band with a molecular size of 200 bps. The size of the amplicon was determined by compare with DNA ladder 100 - 1500 bp. The result of the comparison between observed and anticipated values for SNIP with (rs 1544410) in the tested population was statistically significant, P= < 0.001 and the difference between demographic characteristics and (rs 1544410) SNP, age and BMI shows non-significant difference among all groups. The difference between biomarkers and (rs1544410) SNP was performed using one-way ANOVA test to compare the mean levels of HSP-70, VDBP, C-peptide, RBC and HbA1c% which shown a non-significant difference among the variants of VDBP Genotype (rs1544410) in obese women (patients and control) studied groups, p value > 0.05. Conclusion: The logistic analysis of the (rs1544410) SNP of the patients concluded that HSP-70, VDBP, and C-peptide level was no significantly related to the also C-peptide, was shown to be a related risk factor to both CT and CT alleles (1.003, p > 0.05) in comparison with CC alleles. Furthermore, HbA1c% level was demonstrated to be related as a risk factor for the CG allele in comparison with CC and GG alleles (1.009, p < 0.05).
{"title":"Characteristics of Vitamin D3 Receptor Genotypes in T2DM of Iraqi Obese Women","authors":"Baraa Abdul-Kareem Mutar, T. A. Allwsh, Ammar Yassin, F. J. Al-Tu’ma, H. S. Taghi","doi":"10.22317/jcms.v9i3.1352","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1352","url":null,"abstract":"Objective: This study aims to examine the association between the (rs1544410) polymorphism of the VDR gene with the pathogenesis of T2DM in Iraqi obese women. \u0000Methods: A case-control study was performed on 50 patients with T2DM and 50 apparently healthy subjects who were admitted to Al-Hussein Teaching Hospital and Al-Hassan Center of Diabetes and Endocrinology unit / Kerbala health directorate – Iraq during (April 2022 – March 2023). The T2DM groups were divided into two groups, 25 obese and 25 non-obese; the control group was divided into 25 obese and 25 non-obese as apparently healthy groups. The ELISA Kit was used to measure serum 25(OH)D3, heat shock protein-70, VDBP, insulin and C-peptide. Also HbA1c% and insulin resistance (HOMA-IR) were evaluated. The vitamin D3 receptor gene (VDR) variant and the SNP (rs1544410) polymorphism was determined using allele specific polymerase chain reaction, 1.5% agarose gel electrophoresis and then visualized by gel photo-documentation system. \u0000Results: The result of vitamin D3 variants genotype (rs 1544410) was a clear band with a molecular size of 200 bps. The size of the amplicon was determined by compare with DNA ladder 100 - 1500 bp. The result of the comparison between observed and anticipated values for SNIP with (rs 1544410) in the tested population was statistically significant, P= < 0.001 and the difference between demographic characteristics and (rs 1544410) SNP, age and BMI shows non-significant difference among all groups. The difference between biomarkers and (rs1544410) SNP was performed using one-way ANOVA test to compare the mean levels of HSP-70, VDBP, C-peptide, RBC and HbA1c% which shown a non-significant difference among the variants of VDBP Genotype (rs1544410) in obese women (patients and control) studied groups, p value > 0.05. \u0000Conclusion: The logistic analysis of the (rs1544410) SNP of the patients concluded that HSP-70, VDBP, and C-peptide level was no significantly related to the also C-peptide, was shown to be a related risk factor to both CT and CT alleles (1.003, p > 0.05) in comparison with CC alleles. Furthermore, HbA1c% level was demonstrated to be related as a risk factor for the CG allele in comparison with CC and GG alleles (1.009, p < 0.05).","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46541360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Doaa Alaa Mohammed Hasan, A. Sahib, Ahmed Abbas Hasan Al-Rokan, Karrar Kadhim Mohsin
Objectives: The aim of study was to determine the relationship between this SNPs in postmenopausal women with breast cancer 3435G>A (rs1045642) polymorphism of the ABCB1 gene, and serum concentrations of Paclitaxel chemotherapy in Iraq. Methods: This study included 100 patients (45-75 years) with a clinical diagnosis of breast cancer who were going to start (paclitaxel) chemotherapy. Patients at the oncology center at Imam al-Hussain medical city in Kerbala Iraq, were sorted into groups based on their postmenopausal age, disease duration, and treatment length for this study, which was conducted between July 2022 and October 2022. Serum concentrations of Estradiol and Ca 15.3 were measured in breast cancer patients who had been taking Paclitaxel . Allele-Specific PCR assay determined gene polymorphisms 3435G>A (rs1045642) and HPLC measured paclitaxel drug concentration. Results: The results found There is an inverse relationship between Ca15.3 concentration and patient response to paclitaxel chemotherapy, and when compared to the genotyping frequency of ABCB1 3435G>A (rs1045642) genetic polymorphism, patients with allele AA in their chromosomes respond better (15.56±6.89) than those with alleles GG and GA. Conclusion: After paclitaxel chemotherapy, there were statistically significant differences (p< 0.05) in the reduction of drug concentration among serum patients with the AA genotype for ABCB1 3435G>A (rs1045642).
{"title":"Study the genetic polymorphisms of ABCB1 3435G>A in postmenopausal women breast cancer on Paclitaxel chemotherapy","authors":"Doaa Alaa Mohammed Hasan, A. Sahib, Ahmed Abbas Hasan Al-Rokan, Karrar Kadhim Mohsin","doi":"10.22317/jcms.v9i3.1349","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1349","url":null,"abstract":"Objectives: The aim of study was to determine the relationship between this SNPs in postmenopausal women with breast cancer 3435G>A (rs1045642) polymorphism of the ABCB1 gene, and serum concentrations of Paclitaxel chemotherapy in Iraq. \u0000Methods: This study included 100 patients (45-75 years) with a clinical diagnosis of breast cancer who were going to start (paclitaxel) chemotherapy. Patients at the oncology center at Imam al-Hussain medical city in Kerbala Iraq, were sorted into groups based on their postmenopausal age, disease duration, and treatment length for this study, which was conducted between July 2022 and October 2022. Serum concentrations of Estradiol and Ca 15.3 were measured in breast cancer patients who had been taking Paclitaxel . Allele-Specific PCR assay determined gene polymorphisms 3435G>A (rs1045642) and HPLC measured paclitaxel drug concentration. \u0000Results: The results found There is an inverse relationship between Ca15.3 concentration and patient response to paclitaxel chemotherapy, and when compared to the genotyping frequency of ABCB1 3435G>A (rs1045642) genetic polymorphism, patients with allele AA in their chromosomes respond better (15.56±6.89) than those with alleles GG and GA. \u0000Conclusion: After paclitaxel chemotherapy, there were statistically significant differences (p< 0.05) in the reduction of drug concentration among serum patients with the AA genotype for ABCB1 3435G>A (rs1045642).","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45062306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hazhar M. Balaky, Akam Jasim Mustafa, P. Ismail, A. Yousif
Objectives: This study set out to determine how Calprotectin and B cell activating factor contributes to early COVID-19 patient severity prediction. Methods: The study included 25 healthy controls and 52 patients with SARS-COV2 infection who were clinically diagnosed with COVID-19 illness and were between the ages of 23 and 35. The serum levels of CALP and BAFF were measured using the ELISA method. To gauge CRP levels, an immunoturbidometric assay was performed. Results: Variations in serum levels of CALP and BAFF were found to be statistically insignificant in the study (P=0.7109 & P=0.7575, respectively). When compared to the control group (103.95±36.67 ng/mL; 403.03±1.03), COVID-19 patients had non-significantly raised levels of CALP and BAFF (106.5±4.67 ng/mL; 436.9±12.77 pg/mL, respectively). According to ROC curve analysis, the area under the receiver operating characteristics curve (AUC) for CALP and BAFF was (0.5170) and (0.5259), respectively. (r=0.6923; P=0.0001). There was a significant positive correlation between serum CALP and BAFF levels. The connection between serum CRP levels and CALP (r=0.3010; P=0.1271) and BAFF levels (r=0.2912; P=0.1406) was insignificantly positive. Conclusion: The current study's findings suggested that serum CALP and BAFF concentrations were increased in COVID-19 patients, suggesting that these inflammatory markers may be helpful indicators of the severity of COVID-19.
{"title":"Physiological Effects of Calprotectin and B Cell Activating Factor in COVID-19 Patients","authors":"Hazhar M. Balaky, Akam Jasim Mustafa, P. Ismail, A. Yousif","doi":"10.22317/jcms.v9i3.1347","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1347","url":null,"abstract":"Objectives: This study set out to determine how Calprotectin and B cell activating factor contributes to early COVID-19 patient severity prediction. \u0000Methods: The study included 25 healthy controls and 52 patients with SARS-COV2 infection who were clinically diagnosed with COVID-19 illness and were between the ages of 23 and 35. The serum levels of CALP and BAFF were measured using the ELISA method. To gauge CRP levels, an immunoturbidometric assay was performed. \u0000Results: Variations in serum levels of CALP and BAFF were found to be statistically insignificant in the study (P=0.7109 & P=0.7575, respectively). When compared to the control group (103.95±36.67 ng/mL; 403.03±1.03), COVID-19 patients had non-significantly raised levels of CALP and BAFF (106.5±4.67 ng/mL; 436.9±12.77 pg/mL, respectively). According to ROC curve analysis, the area under the receiver operating characteristics curve (AUC) for CALP and BAFF was (0.5170) and (0.5259), respectively. (r=0.6923; P=0.0001). There was a significant positive correlation between serum CALP and BAFF levels. The connection between serum CRP levels and CALP (r=0.3010; P=0.1271) and BAFF levels (r=0.2912; P=0.1406) was insignificantly positive. \u0000Conclusion: The current study's findings suggested that serum CALP and BAFF concentrations were increased in COVID-19 patients, suggesting that these inflammatory markers may be helpful indicators of the severity of COVID-19.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49623131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Memarian, S. Ghasempouri, Mehran Kouchek, B. Sobouti, K. Aghakhani
Objectives: This study was aimed to evaluate the various demographic and clinical characteristics in hospitalized children with COVID-19 as well as their comorbidities. Methods: This cross-sectional study evaluated a total of 809 hospitalized COVID-19 patients under 18 years of age in the referral university based Ali-Asghar Hospital in 2020. Demographic and clinical characteristics of patients were extracted from the archived records and data analysis was performed using SPSS software version 26. Results: The mean age of patients was 4.1 years and the higher percentage of patients (57%) were male. The most common symptoms of COVID-19 in children were fever, cough, and diarrhea. As well, the most common symptoms in 22.1% of patients admitted to the intensive care unit (ICU) were gastrointestinal (GI) symptoms (79%), fever (62.6%), and respiratory distress (53.6%), respectively. The majority of patients were in the age group less than one year (52%) and mortality rate was 6.3% in total and 10% in children with underlying disease. Besides, the mortality rate of intubated cases was 13 times higher. Conclusion: The findings of present study showed that COVID-19 in children was associated with various clinicopathological manifestations. Underlying disease including respiratory distress, cancer, and kidney disease as well as GI symptoms might be guided predicting the hospitalized cases in ICU. Newborns less than one year of age are exposed to severe COVID-19 infection which is associated with higher mortality rate and it should be given special attention in the early diagnosis and management of the COVID-19 disease.
{"title":"Demographic and Clinical Profiles Including Comorbidities in Hospitalized Patients Under 18 Years of Age with COVID-19","authors":"A. Memarian, S. Ghasempouri, Mehran Kouchek, B. Sobouti, K. Aghakhani","doi":"10.22317/jcms.v9i3.1340","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1340","url":null,"abstract":"Objectives: This study was aimed to evaluate the various demographic and clinical characteristics in hospitalized children with COVID-19 as well as their comorbidities.\u0000Methods: This cross-sectional study evaluated a total of 809 hospitalized COVID-19 patients under 18 years of age in the referral university based Ali-Asghar Hospital in 2020. Demographic and clinical characteristics of patients were extracted from the archived records and data analysis was performed using SPSS software version 26.\u0000Results: The mean age of patients was 4.1 years and the higher percentage of patients (57%) were male. The most common symptoms of COVID-19 in children were fever, cough, and diarrhea. As well, the most common symptoms in 22.1% of patients admitted to the intensive care unit (ICU) were gastrointestinal (GI) symptoms (79%), fever (62.6%), and respiratory distress (53.6%), respectively. The majority of patients were in the age group less than one year (52%) and mortality rate was 6.3% in total and 10% in children with underlying disease. Besides, the mortality rate of intubated cases was 13 times higher.\u0000Conclusion: The findings of present study showed that COVID-19 in children was associated with various clinicopathological manifestations. Underlying disease including respiratory distress, cancer, and kidney disease as well as GI symptoms might be guided predicting the hospitalized cases in ICU. Newborns less than one year of age are exposed to severe COVID-19 infection which is associated with higher mortality rate and it should be given special attention in the early diagnosis and management of the COVID-19 disease.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45992973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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