Israa Khalil Ibrahim Al-Yassiri, F. J. Al-Tu’ma, Maher Abbood Mukheef, Khosay Baqir Jawad, Baraa Abdul-Kareem Mutar
Objective: The aim of the presented word was to assess the diagnostic accuracy of serum Nesfatin-1 in type 2 diabetes mellitus and its relationship with C-peptide level in obese and non-obese type-2 diabetic women of Iraqi population. Methods: A case-control study was performed on 50 type 2 diabetic patients admitted in Al-Hussein Teaching Hospital and Al-Hassan center of diabetes and endocrinology unit / Kerbala health directorate – Iraq and another 50 control individuals, during the period from April, 2022 – Jan. 2023). The T2DM groups were divided into two groups 25 obese and 25 non-obese; also the control group was divided into 25 obese and 25 non-obese as apparently healthy groups. The ELISA Kit was used to measure serum Nesfatin-1 and C-peptide, and random serum glucose was measured by enzymatic colorimetric method, and lipid profile test were measured through spectrophotometric technique, instead of HbA1c% was determined using HPLC method. Results: The results observed indicated that Nesfatin-1 levels shown a non-significant decrease in all of type 2 diabetic groups as compared with apparently healthy control group, while the C-peptide were significantly decreased in type 2 diabetic patients when compared with apparently control group. In addition, the random blood glucose and HbA1c% were shown significant elevation in type 2 diabetic patients as compared with apparently healthy control groups. The observed data indicated that Nesfatin-1 and C-peptide levels when comparing between type 2 diabetic patients and control in obese groups shown a risk factors depending upon the odd ratio observed (OR = 1.064 (1.011-1.119), 1.0200 (0.992-1.08)) respectively, but only Nesfatin-1 was shown to be significant. In BMI the levels of Nesfatin-1 and C-peptide, as shown the Nesfatin-1 was significant in obese groups, while the C-peptide as shown significant in normal weight groups. The optimal diagnostic points for Nesfatin-1 were (sensitivity = 98%, specificity = 90%) at a level (Cut-off points) = 39.13, while C-Peptide levels: (sensitivity = 98%, specificity = 94%) at a level (Cut-off points) = 15.99. Both markers have p-values of the AUC were <0.001 and statistically significant. Conclusion: Accordingly, it was concluded that a significant relationship between circulating Nesfatin-1 levels and type 2 diabetes. Nesfatin-1 appears to be able to contribute to the treatment of obesity and diabetes because of its anorexigenic and antihyperglycemic effects. In addition, C-peptide is a known biomarker of insulin resistance and beta-cell function. High specificity and sensitivity analyzed results were obtained by ROC analysis for both markers in T2DM.
{"title":"Association between Nesfatin-1 Levels and C-Peptide in Sera of Obese / Non-Obese Type 2 Diabetic Women","authors":"Israa Khalil Ibrahim Al-Yassiri, F. J. Al-Tu’ma, Maher Abbood Mukheef, Khosay Baqir Jawad, Baraa Abdul-Kareem Mutar","doi":"10.22317/jcms.v9i1.1322","DOIUrl":"https://doi.org/10.22317/jcms.v9i1.1322","url":null,"abstract":"Objective: The aim of the presented word was to assess the diagnostic accuracy of serum Nesfatin-1 in type 2 diabetes mellitus and its relationship with C-peptide level in obese and non-obese type-2 diabetic women of Iraqi population. \u0000Methods: A case-control study was performed on 50 type 2 diabetic patients admitted in Al-Hussein Teaching Hospital and Al-Hassan center of diabetes and endocrinology unit / Kerbala health directorate – Iraq and another 50 control individuals, during the period from April, 2022 – Jan. 2023). The T2DM groups were divided into two groups 25 obese and 25 non-obese; also the control group was divided into 25 obese and 25 non-obese as apparently healthy groups. The ELISA Kit was used to measure serum Nesfatin-1 and C-peptide, and random serum glucose was measured by enzymatic colorimetric method, and lipid profile test were measured through spectrophotometric technique, instead of HbA1c% was determined using HPLC method. \u0000Results: The results observed indicated that Nesfatin-1 levels shown a non-significant decrease in all of type 2 diabetic groups as compared with apparently healthy control group, while the C-peptide were significantly decreased in type 2 diabetic patients when compared with apparently control group. In addition, the random blood glucose and HbA1c% were shown significant elevation in type 2 diabetic patients as compared with apparently healthy control groups. The observed data indicated that Nesfatin-1 and C-peptide levels when comparing between type 2 diabetic patients and control in obese groups shown a risk factors depending upon the odd ratio observed (OR = 1.064 (1.011-1.119), 1.0200 (0.992-1.08)) respectively, but only Nesfatin-1 was shown to be significant. In BMI the levels of Nesfatin-1 and C-peptide, as shown the Nesfatin-1 was significant in obese groups, while the C-peptide as shown significant in normal weight groups. The optimal diagnostic points for Nesfatin-1 were (sensitivity = 98%, specificity = 90%) at a level (Cut-off points) = 39.13, while C-Peptide levels: (sensitivity = 98%, specificity = 94%) at a level (Cut-off points) = 15.99. Both markers have p-values of the AUC were <0.001 and statistically significant. \u0000Conclusion: Accordingly, it was concluded that a significant relationship between circulating Nesfatin-1 levels and type 2 diabetes. Nesfatin-1 appears to be able to contribute to the treatment of obesity and diabetes because of its anorexigenic and antihyperglycemic effects. In addition, C-peptide is a known biomarker of insulin resistance and beta-cell function. High specificity and sensitivity analyzed results were obtained by ROC analysis for both markers in T2DM.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46787450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Sabaghzadeh, Hamidreza Aslani, Saman Shakeri Jousheghan, M. Aslani, Katayoon Tajik
Objectives: The purpose of present study was the investigation of reliability and validity of the knee injury and osteoarthritis outcome score in the Iranian population. Methods: The method of the present study was non-experimental and methodological. The statistical population of the present study was patients with osteoarthritis of the knee. Patients with osteoarthritis of the knee referred to the Akhtar Center were selected from 127 people by available sampling for sample adequacy. In data analysis, Cronbach's alpha test to determine internal agreement, test-retest method to determine stability in reliability, item impact method to determine face validity, CVI and CVR indicators to determine content validity, and to determine structural validity two convergent and divergent validities as well as confirmatory factor analysis were used. Results: The results showed that if the fourth question "symptoms" is removed, the questionnaire has acceptable reliability and validity. Conclusion: The analysis of the findings indicated that the Persian version of the questionnaire (KOOS) was a reliable tool for assessing the health status and impact of various treatments on patients with osteoarthritis in the country, which had high validity and reliability.
{"title":"Reliability and validity of Knee Injury and Osteoarthritis Outcome Score (KOOS) in Iranian Population","authors":"A. Sabaghzadeh, Hamidreza Aslani, Saman Shakeri Jousheghan, M. Aslani, Katayoon Tajik","doi":"10.22317/jcms.v9i1.1318","DOIUrl":"https://doi.org/10.22317/jcms.v9i1.1318","url":null,"abstract":"Objectives: The purpose of present study was the investigation of reliability and validity of the knee injury and osteoarthritis outcome score in the Iranian population. \u0000Methods: The method of the present study was non-experimental and methodological. The statistical population of the present study was patients with osteoarthritis of the knee. Patients with osteoarthritis of the knee referred to the Akhtar Center were selected from 127 people by available sampling for sample adequacy. In data analysis, Cronbach's alpha test to determine internal agreement, test-retest method to determine stability in reliability, item impact method to determine face validity, CVI and CVR indicators to determine content validity, and to determine structural validity two convergent and divergent validities as well as confirmatory factor analysis were used. Results: The results showed that if the fourth question \"symptoms\" is removed, the questionnaire has acceptable reliability and validity. \u0000Conclusion: The analysis of the findings indicated that the Persian version of the questionnaire (KOOS) was a reliable tool for assessing the health status and impact of various treatments on patients with osteoarthritis in the country, which had high validity and reliability.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49516903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Fadhil Obaid, Zainab Abdulameer Abdulrasol, Ahmed Mohammed Jasim Shlash, Methaq Rabah Tuman, Muamel Dhaher Hussain
Objectives: The study aim is to assess the vaccination status of children under two years old in Al-Asatetha primary health care center / Hilla-Iraq. Method: Retrospective study design (Retrospective chart review) was used to assess the missed opportunities of vaccination for children under the age of two years by reviewing their immunization status from medical reports at the primary health care center. The purposive sampling was used to collect the data of 1000 children born from 1-1-2020 to 31-12-2020 through the period from 1 December 2021 to 1 February 2022. Results: the finding of the current study showed that the age of children in the selected period (2020-2021) was between11-23 months, the number of children (1-8) in the same family, and almost all of these families reside in urban areas. Regarding the parents’ educational level the high percentage of both fathers and mothers were attained a primary level of education, and most of the fathers were working, while most of the mothers were not work. Concerning the vaccination status the finding showed that the percentage of missed vaccination opportunities reached 61%, and there was a significant positive correlation between vaccination status and (family residence, parental education level, and fathers' occupation) with a P value of 0.01. On the other hand, the number and the order of children in families had an inversely significant relationship with vaccination status at a P value of 0.01. Conclusion: the study concludes that numerous factors affect the vaccination rate such as parents' education attainment and awareness, family size, address, and economic status of the family.
{"title":"Assessment of missing opportunity of vaccination at primary health care center: A retrospective study","authors":"Ali Fadhil Obaid, Zainab Abdulameer Abdulrasol, Ahmed Mohammed Jasim Shlash, Methaq Rabah Tuman, Muamel Dhaher Hussain","doi":"10.22317/jcms.v9i1.1321","DOIUrl":"https://doi.org/10.22317/jcms.v9i1.1321","url":null,"abstract":"Objectives: The study aim is to assess the vaccination status of children under two years old in Al-Asatetha primary health care center / Hilla-Iraq. \u0000Method: Retrospective study design (Retrospective chart review) was used to assess the missed opportunities of vaccination for children under the age of two years by reviewing their immunization status from medical reports at the primary health care center. The purposive sampling was used to collect the data of 1000 children born from 1-1-2020 to 31-12-2020 through the period from 1 December 2021 to 1 February 2022. \u0000Results: the finding of the current study showed that the age of children in the selected period (2020-2021) was between11-23 months, the number of children (1-8) in the same family, and almost all of these families reside in urban areas. Regarding the parents’ educational level the high percentage of both fathers and mothers were attained a primary level of education, and most of the fathers were working, while most of the mothers were not work. Concerning the vaccination status the finding showed that the percentage of missed vaccination opportunities reached 61%, and there was a significant positive correlation between vaccination status and (family residence, parental education level, and fathers' occupation) with a P value of 0.01. On the other hand, the number and the order of children in families had an inversely significant relationship with vaccination status at a P value of 0.01. \u0000Conclusion: the study concludes that numerous factors affect the vaccination rate such as parents' education attainment and awareness, family size, address, and economic status of the family.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49573385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The microbiome is a population of microbes that colonized in mammalian gut. During the first few years of life, the gut microbiome undergoes alteration and is very diverse in adulthood, depends upon various of circumstances. Gut microbes, particularly gut flora in ruminants, are receiving more and more attention. Intestinal microbes, particularly ruminant microorganisms, have attracted an increasing amount of attention as high-throughput sequencing technology has improved and costs have decreased, whether in the fundamental research or application fields. The ruminant microbiome changes in conjunction with its host and it is influenced by inter-microbial interactions, environmental exposures, and host properties. However, any organism's core functional microbiome is much more conventional. Unfortunately, the fragile growth ratio of the microbial culture is susceptible to incursions under illness circumstances, which may affect the abundance of various microbial species, resulting to dysbiosis. As a result, the purpose of this review is to provide a broad summary of the relevance of ruminant gut microorganisms, as well as to investigate variables that influence the microbiota and alternative therapeutics such as probiotics, prebiotics, fecal transplantation, and rumen transfiguration, all of which have been shown to be effective in addressing dysbiosis.
{"title":"Ruminant gut microbiota: importance, development, and alternative therapeutics for dysbiosis","authors":"Nahlah Albakri, R. Amasha, M. M. Aly","doi":"10.22317/jcms.v9i1.1301","DOIUrl":"https://doi.org/10.22317/jcms.v9i1.1301","url":null,"abstract":"The microbiome is a population of microbes that colonized in mammalian gut. During the first few years of life, the gut microbiome undergoes alteration and is very diverse in adulthood, depends upon various of circumstances. Gut microbes, particularly gut flora in ruminants, are receiving more and more attention. Intestinal microbes, particularly ruminant microorganisms, have attracted an increasing amount of attention as high-throughput sequencing technology has improved and costs have decreased, whether in the fundamental research or application fields. The ruminant microbiome changes in conjunction with its host and it is influenced by inter-microbial interactions, environmental exposures, and host properties. However, any organism's core functional microbiome is much more conventional. Unfortunately, the fragile growth ratio of the microbial culture is susceptible to incursions under illness circumstances, which may affect the abundance of various microbial species, resulting to dysbiosis. As a result, the purpose of this review is to provide a broad summary of the relevance of ruminant gut microorganisms, as well as to investigate variables that influence the microbiota and alternative therapeutics such as probiotics, prebiotics, fecal transplantation, and rumen transfiguration, all of which have been shown to be effective in addressing dysbiosis.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47286357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Extensive individual variations in the time of onset and severity of the sporadic type of Alzheimer’s disease (AD), may be due to some patient-related external factors. Stress is increasingly recognized as an external factor in the development of AD. Several labs, including mine, have demonstrated that chronic stress or corticosterone administration aggravates the disease in both transgenic and non-transgenic animal models. We have developed a novel rat model that simulates seemingly normal individuals who are predisposed to develop AD. This review summarizes the findings we have reported on the effect of chronic psychosocial stress in this at-risk model of AD. Behavioral (learning and memory tests), electrophysiological (evoked long-term potentiation) and molecular (protein levels of memory-related signaling molecules a well as AD-related molecules. Our findings suggest that even mild psychosocial stress noticeably transforms this seemingly normal rat model to a full-fledge AD phenotype.
{"title":"Chronic psychosocial stress reveals Alzheimer’s disease in a novel at-risk rat model","authors":"Karim A. Alkadhi","doi":"10.22317/jcms.v9i1.1319","DOIUrl":"https://doi.org/10.22317/jcms.v9i1.1319","url":null,"abstract":"Extensive individual variations in the time of onset and severity of the sporadic type of Alzheimer’s disease (AD), may be due to some patient-related external factors. Stress is increasingly recognized as an external factor in the development of AD. Several labs, including mine, have demonstrated that chronic stress or corticosterone administration aggravates the disease in both transgenic and non-transgenic animal models. We have developed a novel rat model that simulates seemingly normal individuals who are predisposed to develop AD. This review summarizes the findings we have reported on the effect of chronic psychosocial stress in this at-risk model of AD. Behavioral (learning and memory tests), electrophysiological (evoked long-term potentiation) and molecular (protein levels of memory-related signaling molecules a well as AD-related molecules. Our findings suggest that even mild psychosocial stress noticeably transforms this seemingly normal rat model to a full-fledge AD phenotype.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47779704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arash Ardestani Zadeh, M. Moonesan, F. Taheri, D. Arab, Tahmineh Mokhtari
Objectives: To compare the analgesic effects of rectal suppository morphine (RSM) with intramuscular morphine (IMM) in patients suffered from renal colic referred to emergency ward (EW). Methods: In a controlled, randomized, clinical trial, 74/90 patients with renal colic referred to the EW between March 2016 and March 2017 were randomly enrolled into two groups of RSM (10 mg) and IMM (10 mg/mL). Vital signs and severity of pain were recorded at admission time (0), 15, 30 and 60 min after treatment. Results: The results showed that there was a significant decrease in VAS score of RSM group compared to IMM group after 30 and 60 min of administration (P˂0.05). Furthermore, no significant difference was recorded in vital signs, except there was a significant decrease in heart rate (15 and 60 min) and respiratory rate (60 min) of RSM group compared to IMM group (P˂0.05) and no side effects were recorded during the investigation. Conclusion: In conclusion, the use of rectal route of morphine had higher efficiency compared to the IM route of morphine in relieving pain of patients with renal colic. Although, decreased heart and respiratory rates were recorded, the values were in normal range. As well, no major complications were recorded for each method.
{"title":"Comparison of rectal suppository and intramuscular morphine for management of patients with renal colic referred to the emergency department: A randomized double-blinded controlled trial","authors":"Arash Ardestani Zadeh, M. Moonesan, F. Taheri, D. Arab, Tahmineh Mokhtari","doi":"10.22317/jcms.v9i1.1316","DOIUrl":"https://doi.org/10.22317/jcms.v9i1.1316","url":null,"abstract":"Objectives: To compare the analgesic effects of rectal suppository morphine (RSM) with intramuscular morphine (IMM) in patients suffered from renal colic referred to emergency ward (EW). \u0000Methods: In a controlled, randomized, clinical trial, 74/90 patients with renal colic referred to the EW between March 2016 and March 2017 were randomly enrolled into two groups of RSM (10 mg) and IMM (10 mg/mL). Vital signs and severity of pain were recorded at admission time (0), 15, 30 and 60 min after treatment. \u0000Results: The results showed that there was a significant decrease in VAS score of RSM group compared to IMM group after 30 and 60 min of administration (P˂0.05). Furthermore, no significant difference was recorded in vital signs, except there was a significant decrease in heart rate (15 and 60 min) and respiratory rate (60 min) of RSM group compared to IMM group (P˂0.05) and no side effects were recorded during the investigation. \u0000Conclusion: In conclusion, the use of rectal route of morphine had higher efficiency compared to the IM route of morphine in relieving pain of patients with renal colic. Although, decreased heart and respiratory rates were recorded, the values were in normal range. As well, no major complications were recorded for each method.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46721381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Eslami, Majid Golshanfard, Amir Bajouri, N. Aghdami, Mahsa Mohammadi, S. Shafieian, Omeed Memarsadeghi, A. Seifalian
Objectives: This clinical research aimed to establish autologous fibroblasts transplantation as a possible treatment for patients with DEJ. The COL7A1 gene expression was also evaluated. Methods: Six patients (3M and 3F), 4 with no recurrent wounds and 2 with recurrent wounds after surgery, and 15 healthy subjects were included in the study as controls. Quantitative real-time polymerase chain reaction (real-time PCR) analysis of the COL7A1 gene was performed using an oligonucleotide primer pair designed to amplify across the exon/exon junction. Results: The COL7A1 expression level was down-regulated at exons 26-27, 47-48, 96-97, and 116-117 in all patients′ fibroblasts compared with the healthy controls. However, the expression of the COL7A1 gene in the fibroblasts of the patients with a positive response to the treatment was not significantly changed compared with the patients with the poor response. (ClinicalTrials.gov NCT01908088) Conclusion: In this study the mRNA expression levels of COL7A1 were significantly less in the patients when compared with healthy controls. However the COL7A1 expression after autologous fibroblasts transplantation was not different between the two groups of patients, and further examination is needed to elucidate the mechanism of the treatment.
{"title":"Comparing COL7A1 gene expression in fibroblast cells of dystrophic epidermolysis bullosa patients with clinical responses to autologous fibroblasts transplantation","authors":"M. Eslami, Majid Golshanfard, Amir Bajouri, N. Aghdami, Mahsa Mohammadi, S. Shafieian, Omeed Memarsadeghi, A. Seifalian","doi":"10.22317/jcms.v9i1.1314","DOIUrl":"https://doi.org/10.22317/jcms.v9i1.1314","url":null,"abstract":"Objectives: This clinical research aimed to establish autologous fibroblasts transplantation as a possible treatment for patients with DEJ. The COL7A1 gene expression was also evaluated. \u0000Methods: Six patients (3M and 3F), 4 with no recurrent wounds and 2 with recurrent wounds after surgery, and 15 healthy subjects were included in the study as controls. Quantitative real-time polymerase chain reaction (real-time PCR) analysis of the COL7A1 gene was performed using an oligonucleotide primer pair designed to amplify across the exon/exon junction. \u0000Results: The COL7A1 expression level was down-regulated at exons 26-27, 47-48, 96-97, and 116-117 in all patients′ fibroblasts compared with the healthy controls. However, the expression of the COL7A1 gene in the fibroblasts of the patients with a positive response to the treatment was not significantly changed compared with the patients with the poor response. (ClinicalTrials.gov NCT01908088) \u0000Conclusion: In this study the mRNA expression levels of COL7A1 were significantly less in the patients when compared with healthy controls. However the COL7A1 expression after autologous fibroblasts transplantation was not different between the two groups of patients, and further examination is needed to elucidate the mechanism of the treatment.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48324789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives: AML is the only type of acute leukemia diagnosed in adults and is less common in children. It has the lowest survival rate. This study aimed to investigate the epidemiological risk factors for AML expansion comprise environmental factors, for instance, smoking , and therapy-related factors. Methods :The study was conducted on 70 acute myeloid leukemia patients—37 females and 33 males and on 30 healthy people—12 females and 18 males—as a control group. DNA was extracted from the study groups' whole blood samples using the gSYNCTM DNA Extraction Kit. The T751G polymorphism of the ERCC2 gene was determined by the PCR-RFLP technique. Results: In genetic analysis, it was shown that the carriers of allele Lys and genotype Lys/Lys have a lower risk of developing AML, while allele carriers Gln have an increased risk. The results showed the ERCC2 gene, Lys 751 Gln (T/G) heterozygous TG genotypes, and the G allele were significantly higher (P<0.05) in AML patients compared to the control group. In the sequencing of the region we studied, it was found that there is a site of diversity that is located between the CTTCAG and CTGCAG, where a change in nucleotides (T to G) represents the restriction site of the restriction enzyme. Conclusion : The polymorphic marker 751 Gln> Lys of the ERCC2 gene was associated with the development of AML in Iraqi patients. It was discovered that allele Lys genotype Lys/Lys carriers have a lower risk of developing AML, whereas allele Gln carriers have an increased risk.
{"title":"The impact of the ERCC2 Lys751Gln polymorphism on the risk of acute myeloid leukemia in an Iraqi patients","authors":"Thamer Mouhi Jasiem, R. Al-Hussaini","doi":"10.22317/jcms.v9i1.1308","DOIUrl":"https://doi.org/10.22317/jcms.v9i1.1308","url":null,"abstract":"Objectives: AML is the only type of acute leukemia diagnosed in adults and is less common in children. It has the lowest survival rate. This study aimed to investigate the epidemiological risk factors for AML expansion comprise environmental factors, for instance, smoking , and therapy-related factors.\u0000Methods :The study was conducted on 70 acute myeloid leukemia patients—37 females and 33 males and on 30 healthy people—12 females and 18 males—as a control group. DNA was extracted from the study groups' whole blood samples using the gSYNCTM DNA Extraction Kit. The T751G polymorphism of the ERCC2 gene was determined by the PCR-RFLP technique.\u0000Results: In genetic analysis, it was shown that the carriers of allele Lys and genotype Lys/Lys have a lower risk of developing AML, while allele carriers Gln have an increased risk. The results showed the ERCC2 gene, Lys 751 Gln (T/G) heterozygous TG genotypes, and the G allele were significantly higher (P<0.05) in AML patients compared to the control group. In the sequencing of the region we studied, it was found that there is a site of diversity that is located between the CTTCAG and CTGCAG, where a change in nucleotides (T to G) represents the restriction site of the restriction enzyme.\u0000Conclusion : The polymorphic marker 751 Gln> Lys of the ERCC2 gene was associated with the development of AML in Iraqi patients. It was discovered that allele Lys genotype Lys/Lys carriers have a lower risk of developing AML, whereas allele Gln carriers have an increased risk. ","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48257371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hadeel Abdul Latif Jouad, Shatha Abdul Wadood AL- Shammaree
Objectives: The purpose of study was to explore the correlation of advanced oxidation protein products (AOPP), which reflect the oxidation of protein and the oxidative stress status, and the activity of antioxidant enzyme of Paraoxonase (PON1), using its arylesterase activity in patients with thyrodisim. Methods: The study included 100 women with newly diagnosed thyrodisim were subdivided in two groups according to thyroid hormones levels: hyperthyroidism group (50 female patients, age range 18-60 years); and hypothyroidism group (50 female patients, age range 18-75 years). A control group (30 healthy females, age range 18–70 years) was also included for comparison. Demographic and clinical measurements for all participants were recorded which include: Body mass index (BMI), age, weight, height, lipid profile, vitamin D, thyroid hormones (TSH, T3, and T4, FT3, FT4), AOPP levels, and arylesterase activity. Results: The serum level of AOPP in hypothyroidism group (71.92±19.04 μmol/L) and in hyperthyroidism group (30.41±4.72 μmol/L) were significantly higher than controls (13.12±2.50 μmol/L) (P<0.05). In contrast, lower aryl esterase activity was found in hypothyroidism (5.03±0.50 U/L), and hyperthyroidism (3.64±0.40 U/L) when compared to control group (6.78±0.62 Ku/L) with significant values (P< 0.05). Conclusions: these results disclosed a significant role of protein oxidation in patients with hypothyroidism as well as the oxidative stress status.
{"title":"Advanced oxidation protein products levels and Paraoxonase 1(Arylesterase) activity in patients with thyrodisim","authors":"Hadeel Abdul Latif Jouad, Shatha Abdul Wadood AL- Shammaree","doi":"10.22317/jcms.v9i1.1317","DOIUrl":"https://doi.org/10.22317/jcms.v9i1.1317","url":null,"abstract":"Objectives: The purpose of study was to explore the correlation of advanced oxidation protein products (AOPP), which reflect the oxidation of protein and the oxidative stress status, and the activity of antioxidant enzyme of Paraoxonase (PON1), using its arylesterase activity in patients with thyrodisim. \u0000Methods: The study included 100 women with newly diagnosed thyrodisim were subdivided in two groups according to thyroid hormones levels: hyperthyroidism group (50 female patients, age range 18-60 years); and hypothyroidism group (50 female patients, age range 18-75 years). A control group (30 healthy females, age range 18–70 years) was also included for comparison. Demographic and clinical measurements for all participants were recorded which include: Body mass index (BMI), age, weight, height, lipid profile, vitamin D, thyroid hormones (TSH, T3, and T4, FT3, FT4), AOPP levels, and arylesterase activity. \u0000Results: The serum level of AOPP in hypothyroidism group (71.92±19.04 μmol/L) and in hyperthyroidism group (30.41±4.72 μmol/L) were significantly higher than controls (13.12±2.50 μmol/L) (P<0.05). In contrast, lower aryl esterase activity was found in hypothyroidism (5.03±0.50 U/L), and hyperthyroidism (3.64±0.40 U/L) when compared to control group (6.78±0.62 Ku/L) with significant values (P< 0.05). \u0000Conclusions: these results disclosed a significant role of protein oxidation in patients with hypothyroidism as well as the oxidative stress status.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42983895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hadbaa H. Al-Murshedi, Ammar Fadhil Jawad, F. J. Al-Tu’ma, E. Hadi, M. M. K. Al-Tu’ma
Objectives: To explore an association between CAPN10, SNP-44 (rs2975760) with IR condition in women with PCOS. Methods: A study included 120 participants of which 68 women have PCOS subdivide according to their body mass index (BMI) into 45 obese (BMI≥30) and 23 non-obese (BMI<30). The remaining 52 represent the control group who were apparently healthy women with normal weight and normal menstrual cycle. Patients with PCOS were selected from the Infertility Department, Gynecology and Obstetrics Teaching Hospital, Kerbala Health Directorate / Kerbala-Iraq between Nov., 2021 and June, 2022. Diagnosis of PCOS is based on 2 of 3 findings: oligo/anovulation, hyperandrogenism, polycystic ovaries in ultrasound (Rotterdam criteria). Patients were interviewed and examined for weight, height, waist circumference, and hip circumference. Venous blood samples were collected at 9 AM after an overnight fast. IR was assessed by calculating homeostatic model assessment of insulin resistance (HOMA-IR) using the formula (fasting glucose mg/dl x fasting insulin µU/ml)/405, taking normal value <2.7. Genotypes of CAPN10, SNP-44 has been identified using Allele-specific polymerase chain reaction (AS-PCR) technique. Results: The prevalence of IR based on HOMA-IR was (80%) in obese PCOS and (48%) in non-obese PCOS women. CAPN10, SNP-44 has been reconstructed and analyzed in patients and controls. Genotypes of 45 obese PCOS subjects (TT, N=26; TC, N=12; and CC, N=7), 23 non-obese PCOS subjects (TT, N=15; TC, N=6; and CC, N=2) and control subjects (TT, N=39; TC, N=11; and CC, N=2) were identified. The genotype distribution was statistically different between obese PCOS women and controls (OR=5.25, P=0.048). The association of SNP-44 allele with IR status was detected. HOMA-IR was greater in CC (10.54 ± 1.29, 9.88 ± 1.41) than in TT (3.30 ± 1.52, P<0.001; 2.82 ± 1.45, P<0.001) and TC (3.76 ± 1.58, P<0.001; 4.10 ±1.57, P<0.05) in obese PCOS and non-obese PCOS subjects respectively. Conclusion: In obese PCOS, the C allele was associated with higher insulin secretion and HOMA-IR compared with the T allele. The increased HOMA-IR is an indicator of IR. In this scenario, the C allele might be involved in the pathophysiology of insulin resistance in PCOS.
目的:探讨CAPN10、SNP-44 (rs2975760)与PCOS女性IR状况的关系。方法:120名多囊卵巢综合征(PCOS)患者按体重指数(BMI)分为肥胖45例(BMI≥30)和非肥胖23例(BMI<30)。剩下的52人是对照组，她们显然是体重正常、月经周期正常的健康女性。多囊卵巢综合征患者于2021年11月至2022年6月在克尔巴拉卫生局/克尔巴拉-伊拉克妇产科教学医院不孕症科选择。多囊卵巢综合征的诊断基于以下3个表现中的2个:超声显示的少/无排卵、高雄激素症、多囊卵巢(鹿特丹标准)。对患者进行访谈并检查体重、身高、腰围和臀围。禁食一夜后于上午9点采集静脉血。IR通过计算胰岛素抵抗稳态模型评估(HOMA-IR)，计算公式为(空腹葡萄糖mg/dl x空腹胰岛素µU/ml)/405，取正常值<2.7。利用等位基因特异性聚合酶链反应(AS-PCR)技术鉴定了CAPN10、SNP-44的基因型。结果:基于HOMA-IR的IR在肥胖PCOS患者中的患病率为(80%)，在非肥胖PCOS患者中患病率为(48%)。在患者和对照组中重建并分析了CAPN10、SNP-44。45例肥胖PCOS患者基因型分析(TT, N=26;TC, N = 12;和CC, N=7)，非肥胖PCOS患者23例(TT, N=15;TC, N = 6;CC组，N=2)和对照组(TT组，N=39;TC, N = 11;和CC, N=2)。肥胖PCOS女性与对照组基因型分布差异有统计学意义(OR=5.25, P=0.048)。检测到SNP-44等位基因与IR状态的关联。CC组HOMA-IR(10.54±1.29,9.88±1.41)高于TT组(3.30±1.52,P<0.001);(2.82±1.45,P<0.001)、TC(3.76±1.58,P<0.001);肥胖型PCOS与非肥胖型PCOS分别为4.10±1.57 (P<0.05)。结论:在肥胖型多囊卵巢综合征中，与T等位基因相比，C等位基因与较高的胰岛素分泌和HOMA-IR相关。HOMA-IR升高是IR的一个指标。在这种情况下，C等位基因可能参与多囊卵巢综合征胰岛素抵抗的病理生理。
{"title":"Molecular studies of CAPN-10 gene (rs2975760) and its association with Insulin Resistance in Polycystic Ovarian Syndrome of Iraqi women","authors":"Hadbaa H. Al-Murshedi, Ammar Fadhil Jawad, F. J. Al-Tu’ma, E. Hadi, M. M. K. Al-Tu’ma","doi":"10.22317/jcms.v8i6.1298","DOIUrl":"https://doi.org/10.22317/jcms.v8i6.1298","url":null,"abstract":"Objectives: To explore an association between CAPN10, SNP-44 (rs2975760) with IR condition in women with PCOS. \u0000Methods: A study included 120 participants of which 68 women have PCOS subdivide according to their body mass index (BMI) into 45 obese (BMI≥30) and 23 non-obese (BMI<30). The remaining 52 represent the control group who were apparently healthy women with normal weight and normal menstrual cycle. Patients with PCOS were selected from the Infertility Department, Gynecology and Obstetrics Teaching Hospital, Kerbala Health Directorate / Kerbala-Iraq between Nov., 2021 and June, 2022. Diagnosis of PCOS is based on 2 of 3 findings: oligo/anovulation, hyperandrogenism, polycystic ovaries in ultrasound (Rotterdam criteria). Patients were interviewed and examined for weight, height, waist circumference, and hip circumference. Venous blood samples were collected at 9 AM after an overnight fast. IR was assessed by calculating homeostatic model assessment of insulin resistance (HOMA-IR) using the formula (fasting glucose mg/dl x fasting insulin µU/ml)/405, taking normal value <2.7. Genotypes of CAPN10, SNP-44 has been identified using Allele-specific polymerase chain reaction (AS-PCR) technique. \u0000Results: The prevalence of IR based on HOMA-IR was (80%) in obese PCOS and (48%) in non-obese PCOS women. CAPN10, SNP-44 has been reconstructed and analyzed in patients and controls. Genotypes of 45 obese PCOS subjects (TT, N=26; TC, N=12; and CC, N=7), 23 non-obese PCOS subjects (TT, N=15; TC, N=6; and CC, N=2) and control subjects (TT, N=39; TC, N=11; and CC, N=2) were identified. The genotype distribution was statistically different between obese PCOS women and controls (OR=5.25, P=0.048). The association of SNP-44 allele with IR status was detected. HOMA-IR was greater in CC (10.54 ± 1.29, 9.88 ± 1.41) than in TT (3.30 ± 1.52, P<0.001; 2.82 ± 1.45, P<0.001) and TC (3.76 ± 1.58, P<0.001; 4.10 ±1.57, P<0.05) in obese PCOS and non-obese PCOS subjects respectively. \u0000Conclusion: In obese PCOS, the C allele was associated with higher insulin secretion and HOMA-IR compared with the T allele. The increased HOMA-IR is an indicator of IR. In this scenario, the C allele might be involved in the pathophysiology of insulin resistance in PCOS.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41389668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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