Pub Date : 2025-04-12DOI: 10.1016/j.saa.2025.126182
Changwei Jiao , Miaoliang Chen , Jiaqi Liao , Jialun Li , Ruili Zhang , Sailing He
A self-developed aberration-free line scanning confocal Raman imager (AFLSCRI) with a spectral resolution of 0.12 nm and a spatial resolution of 2 μm is utilized to diagnose colorectal cancer. The tissues were categorized into four subgroups (typical tissue, lipid-rich tissue, fat-rich tissue, and collagen-rich tissue) and were successfully distinguished with our Raman imaging results. Compared to traditional point-scanning Raman spectroscopy, this imager offers a much faster speed with high spectral resolution while maintaining a similar spatial resolution. The Raman spectroscopy results of the same sample of colorectal cancer remain stable and unaffected even measured after six months. The molecular composition of the tissues was analyzed, and potential biomarkers such as carotenoids and protein structures were identified for four different types of colorectal tissues. When combined with machine learning algorithms, an accuracy of 92.8% was achieved in identifying 14 pairs of normal/cancer samples. These results highlight the great potential of the AFLSCRI in label-free, rapid, and non-invasive medical diagnosis.
{"title":"Rapid, label-free detection of colorectal cancer with an aberration-free line scanning confocal Raman imager","authors":"Changwei Jiao , Miaoliang Chen , Jiaqi Liao , Jialun Li , Ruili Zhang , Sailing He","doi":"10.1016/j.saa.2025.126182","DOIUrl":"10.1016/j.saa.2025.126182","url":null,"abstract":"<div><div>A self-developed aberration-free line scanning confocal Raman imager (AFLSCRI) with a spectral resolution of 0.12 nm and a spatial resolution of 2 μm is utilized to diagnose colorectal cancer. The tissues were categorized into four subgroups (typical tissue, lipid-rich tissue, fat-rich tissue, and collagen-rich tissue) and were successfully distinguished with our Raman imaging results. Compared to traditional point-scanning Raman spectroscopy, this imager offers a much faster speed with high spectral resolution while maintaining a similar spatial resolution. The Raman spectroscopy results of the same sample of colorectal cancer remain stable and unaffected even measured after six months. The molecular composition of the tissues was analyzed, and potential biomarkers such as carotenoids and protein structures were identified for four different types of colorectal tissues. When combined with machine learning algorithms, an accuracy of 92.8% was achieved in identifying 14 pairs of normal/cancer samples. These results highlight the great potential of the AFLSCRI in label-free, rapid, and non-invasive medical diagnosis.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"339 ","pages":"Article 126182"},"PeriodicalIF":4.3,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143842993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-12DOI: 10.1016/j.saa.2025.126208
Wen Liu , Jun Dong , Yuchong Ren , Wanting Zhou , Qingyan Han , Chengyun Zhang , Kaili Ren , Yongkai Wang , Wei Gao , Jianxia Qi
In this paper, Au-Ag alloy nanostar (Au-Ag NSt) substrates were prepared by liquid-liquid three-phase self-assembly method, and the influence of preparation conditions on their Raman activity of the substrate was explored. By adjusting the gold-silver ratios of alloy seeds and the concentration of AgNO3 in the growth solution, it was found that the LSPR peak can be adjusted. Under the conditions of Au1-Ag3 nanoparticles as seeds and 200 μL AgNO3 solution (2 mM) as epitaxial growth solution, the prepared Au1-Ag3 NSt substrate exhibits excellent SERS activity. The substrate can detect R6G and CV probe molecules with detection limits as low as 10−11 M and 10−10 M, respectively. Experiments have confirmed that the substrate has the characteristics of high sensitivity, good uniformity and strong stability. In addition, the substrate can detect APM molecules far below the safety standard, which further proves that the structure has excellent practical application potential as a SERS substrate.
{"title":"Fabrication of plasmonic Au-Ag alloy nanostars for ultrasensitive SERS detection","authors":"Wen Liu , Jun Dong , Yuchong Ren , Wanting Zhou , Qingyan Han , Chengyun Zhang , Kaili Ren , Yongkai Wang , Wei Gao , Jianxia Qi","doi":"10.1016/j.saa.2025.126208","DOIUrl":"10.1016/j.saa.2025.126208","url":null,"abstract":"<div><div>In this paper, Au-Ag alloy nanostar (Au-Ag NSt) substrates were prepared by liquid-liquid three-phase self-assembly method, and the influence of preparation conditions on their Raman activity of the substrate was explored. By adjusting the gold-silver ratios of alloy seeds and the concentration of AgNO<sub>3</sub> in the growth solution, it was found that the LSPR peak can be adjusted. Under the conditions of Au<sub>1</sub>-Ag<sub>3</sub> nanoparticles as seeds and 200 μL AgNO<sub>3</sub> solution (2 mM) as epitaxial growth solution, the prepared Au<sub>1</sub>-Ag<sub>3</sub> NSt substrate exhibits excellent SERS activity. The substrate can detect R6G and CV probe molecules with detection limits as low as 10<sup>−11</sup> M and 10<sup>−10</sup> M, respectively. Experiments have confirmed that the substrate has the characteristics of high sensitivity, good uniformity and strong stability. In addition, the substrate can detect APM molecules far below the safety standard, which further proves that the structure has excellent practical application potential as a SERS substrate.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"339 ","pages":"Article 126208"},"PeriodicalIF":4.3,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Supramolecularly stacked acriflavine dye on graphene oxide (GO-Acy) was utilized for the first time to detect aliphatic amines, making it a highly sensitive and selective material. This method involved developing a distinctive, discriminative, and highly selective fluorescent sensor that displayed a ’turn-on’ response to aliphatic amines. The sensor probe operated through supramolecular host–guest interactions between the amines and dyes, enabling the detection of aliphatic amines. Upon the interaction of aliphatic amines with bound Acy dye on graphene oxide, the fluorescent sensor exhibited a release of Acy dyes and a rapid appearance of fluorescence. GO-Acy demonstrated high selectivity for 1,4-butanediamine (BD), exhibiting strong fluorescence intensity and accurately distinguishing it from various aliphatic amines, aromatic amines, and amino acids. The fluorescene emission intensities of GO-Acy at λem = 510 nm was observed after exposure to different amines, with an excitation wavelength of GO-Acy at λex = 450 nm. In addition to its high selectivity, the sensor probe also exhibited excellent sensitivity towards BD, with a limit of detection (LOD) of 9.9 nM. This graphene-based material proved to be a highly effective tool for detecting aliphatic amines in aqueous media, requiring no special experiments while providing both good selectivity and sensitivity. Additionally, GO-Acy@CB[7] demonstrated diverse responses to aliphatic amines, suggesting its ability to discriminate between them through fluorescence quenching. GO-Acy has proven effective in monitoring fish products that release amine vapors during decomposition. Furthermore, test paper strips made with GO-Acy could quickly detect amines in actual fish samples, highlighting the potential of GO-Acy for food quality inspection.
{"title":"Aliphatic amines – Sensing application in aqueous media using supramolecularly stacked acriflavine dye on graphene oxide","authors":"Pavitra Rajendran , Jamuna Kannan , Lakshminarayanan Piramuthu , Erumaipatty Rajagounder Nagarajan , Shanmugam Sivakumar , Narayanan Selvapalam","doi":"10.1016/j.saa.2025.126233","DOIUrl":"10.1016/j.saa.2025.126233","url":null,"abstract":"<div><div>Supramolecularly stacked acriflavine dye on graphene oxide (GO-Acy) was utilized for the first time to detect aliphatic amines, making it a highly sensitive and selective material. This method involved developing a distinctive, discriminative, and highly selective fluorescent sensor that displayed a ’turn-on’ response to aliphatic amines. The sensor probe operated through supramolecular host–guest interactions between the amines and dyes, enabling the detection of aliphatic amines. Upon the interaction of aliphatic amines with bound Acy dye on graphene oxide, the fluorescent sensor exhibited a release of Acy dyes and a rapid appearance of fluorescence. GO-Acy demonstrated high selectivity for 1,4-butanediamine (BD), exhibiting strong fluorescence intensity and accurately distinguishing it from various aliphatic amines, aromatic amines, and amino acids. The fluorescene emission intensities of GO-Acy at λ<sub>em</sub> = 510 nm was observed after exposure to different amines, with an excitation wavelength of GO-Acy at λ<sub>ex</sub> = 450 nm. In addition to its high selectivity, the sensor probe also exhibited excellent sensitivity towards BD, with a limit of detection (LOD) of 9.9 nM. This graphene-based material proved to be a highly effective tool for detecting aliphatic amines in aqueous media, requiring no special experiments while providing both good selectivity and sensitivity. Additionally, GO-Acy@CB[7] demonstrated diverse responses to aliphatic amines, suggesting its ability to discriminate between them through fluorescence quenching. GO-Acy has proven effective in monitoring fish products that release amine vapors during decomposition. Furthermore, test paper strips made with GO-Acy could quickly detect amines in actual fish samples, highlighting the potential of GO-Acy for food quality inspection.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"338 ","pages":"Article 126233"},"PeriodicalIF":4.3,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-12DOI: 10.1016/j.saa.2025.126235
Qinrong Yang , Sha Li , Hong Li , Shengping Liu , Ningjin Zhang , Tao Sun , Xiaoping Bao
The design of synthetic anion receptors with potent anion binding and customizable anion selectivity under competitive solvent conditions remains challenging. Herein, we report easily-synthesized 1,8-disulfonamidocarbazole- and 3,5-diamidopyridine-based hybrid macrocycles 1−3 and reveal their strong anion recognition properties as determined by 1H NMR/UV−vis titration studies, X-ray diffraction measurements, and DFT calculations. While the dithioamidopyridine-based macrocycle 2 displayed strong and selective binding of AcO− in DMSO, modification of the selectivity pattern towards the more basic F− anion was achieved by replacing the thioamides moieties to amides (macrocycle 1). For macrocycle 3 (bearing pyridine N-oxide core), no selectivity was observed among F−, AcO−, and H2PO4− ions. The demonstration of tunable anion selectivity by slight structural modifications in our macrocycles is informative for developing structurally simple anion receptors with the desired selectivity for transmembrane anion transport, anion sensing, and anion sequestration applications.
{"title":"Carbazole-disulfonamide-containing macrocycles as powerful anion receptors with tunable selectivity","authors":"Qinrong Yang , Sha Li , Hong Li , Shengping Liu , Ningjin Zhang , Tao Sun , Xiaoping Bao","doi":"10.1016/j.saa.2025.126235","DOIUrl":"10.1016/j.saa.2025.126235","url":null,"abstract":"<div><div>The design of synthetic anion receptors with potent anion binding and customizable anion selectivity under competitive solvent conditions remains challenging. Herein, we report easily-synthesized 1,8-disulfonamidocarbazole- and 3,5-diamidopyridine-based hybrid macrocycles <strong>1</strong>−<strong>3</strong> and reveal their strong anion recognition properties as determined by <sup>1</sup>H NMR/UV−vis titration studies, X-ray diffraction measurements, and DFT calculations. While the dithioamidopyridine-based macrocycle <strong>2</strong> displayed strong and selective binding of AcO<sup>−</sup> in DMSO, modification of the selectivity pattern towards the more basic F<sup>−</sup> anion was achieved by replacing the thioamides moieties to amides (macrocycle <strong>1</strong>). For macrocycle <strong>3</strong> (bearing pyridine <em>N</em>-oxide core), no selectivity was observed among F<sup>−</sup>, AcO<sup>−</sup>, and H<sub>2</sub>PO<sub>4</sub><sup>−</sup> ions. The demonstration of tunable anion selectivity by slight structural modifications in our macrocycles is informative for developing structurally simple anion receptors with the desired selectivity for transmembrane anion transport, anion sensing, and anion sequestration applications.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"339 ","pages":"Article 126235"},"PeriodicalIF":4.3,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143842990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The recognition of amino acid enantiomers is of great significance in drug research and disease diagnosis. However, the similar physical and chemical properties of enantiomers make chiral recognition challenging. Optical characterization is a promising approach to distinguish enantiomers. Carbon dots (CDs) have attracted widespread attention due to their simple synthesis, high economic benefits, and good biocompatibility. Chiral carbon dots, as fluorescent probes, have gradually received more attraction. Here, a fluorescence probe (CYS-CDs + Cu2+) was constructed using D-Cys as chiral source to synthesize chiral carbon dots (CYS-CDs), and Cu2+ as inducer to recognize the chirality of lysine enantiomers(L-/D-Lys). And then, based on the ability of Cu2+ to quench the fluorescence of CYS-CDs and L-Lysine (L-Lys) could restore the fluorescence of the CYS-CDs + Cu2+ probe, so achieve an “on–off–on” detection mode to detect L-Lys. On the contrary, D-Lys cannot restore the fluorescence of CYS-CDs + Cu2+. The probe can distinguish lysine enantiomers not only by fluorescence spectra but also by circular dichroism spectra. And, the probe can be used for the quantitative detection of L-Lys by fluorescence method, of which the detection range is 0–520 μM, and the limit of detection is only 13.70 μM. In addition, a paper-based fluorescent senor was constructed on this basis. This work may be of practical significance to expand the recognition method of amino acid enantiomers.
{"title":"One-pot synthesis of fluorescent nanoprobes based on D-cys-based CDs and quantitative detection of lysine enantiomers","authors":"Jinqiu Li, Fanghao Wang, Hao Zhang, Duxia Cao, Ruifang Guan","doi":"10.1016/j.saa.2025.126143","DOIUrl":"10.1016/j.saa.2025.126143","url":null,"abstract":"<div><div>The recognition of amino acid enantiomers is of great significance in drug research and disease diagnosis. However, the similar physical and chemical properties of enantiomers make chiral recognition challenging. Optical characterization is a promising approach to distinguish enantiomers. Carbon dots (CDs) have attracted widespread attention due to their simple synthesis, high economic benefits, and good biocompatibility. Chiral carbon dots, as fluorescent probes, have gradually received more attraction. Here, a fluorescence probe (CYS-CDs + Cu<sup>2+</sup>) was constructed using D-Cys as chiral source to synthesize chiral carbon dots (CYS-CDs), and Cu<sup>2+</sup> as inducer to recognize the chirality of lysine enantiomers(L-/D-Lys). And then, based on the ability of Cu<sup>2+</sup> to quench the fluorescence of CYS-CDs and L-Lysine (L-Lys) could restore the fluorescence of the CYS-CDs + Cu<sup>2+</sup> probe, so achieve an “on–off–on” detection mode to detect L-Lys. On the contrary, D-Lys cannot restore the fluorescence of CYS-CDs + Cu<sup>2+</sup>. The probe can distinguish lysine enantiomers not only by fluorescence spectra but also by circular dichroism spectra. And, the probe can be used for the quantitative detection of L-Lys by fluorescence method, of which the detection range is 0–520 μM, and the limit of detection is only 13.70 μM. In addition, a paper-based fluorescent senor was constructed on this basis. This work may be of practical significance to expand the recognition method of amino acid enantiomers.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"339 ","pages":"Article 126143"},"PeriodicalIF":4.3,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-11DOI: 10.1016/j.saa.2025.126223
Li Xuecheng, Hao Haoyue, Li Liang
In this study, the intensity and properties of up-conversion luminescence in core–shell structured NaGdF4: Er3+/Tm3+/Yb3+@NaGdF4: Yb3+ phosphors were investigated. After coating the NaGdF4: 0.5Er3+/0.5Tm3+/30Yb3+ (0.5Er/0.5Tm/30Yb) core with NaGdF4: xYb3+ (x = 25, 50) shells, the luminescence intensity of the phosphors was improved. The enhancement factor reached to 3.97 (x = 25) and 1.15 (x = 5) respectively. Owing to the different increase of up-conversion luminescence peak, the emission color changed from blue-green to yellowish-green in NaGdF4: 0.5Er3+/0.5Tm3+/30Yb3+@NaGdF4: 25Yb3+ (0.5Er/0.5Tm/30Yb@25Yb) phosphors. Additionally, the temperature sensing properties of the 0.5Er/0.5Tm/30Yb@25Yb phosphors were examined. A maximum relative sensitivity (Sr) of 0.013 K−1 was observed at 303 K based on florescence intensity ratio of two green emission. And a higher Sr of 0.034 K−1 was recorded at 403 K based on the ratio of green (540 nm) and near-red emissions (800 nm). These findings not only deepen the understanding of the up-conversion processes in core–shell structured phosphors but also promote the development of rare-earth ion core–shell structures for applications in temperature sensing and luminescent displays.
{"title":"Up-conversion emission characteristics and temperature measurement of core-shell structured NaGdF4: Er3+Tm3+/Yb3+@NaYbF4: xYb3+ (x = 25, 50) phosphors","authors":"Li Xuecheng, Hao Haoyue, Li Liang","doi":"10.1016/j.saa.2025.126223","DOIUrl":"10.1016/j.saa.2025.126223","url":null,"abstract":"<div><div>In this study, the intensity and properties of up-conversion luminescence in core–shell structured NaGdF<sub>4</sub>: Er<sup>3+</sup>/Tm<sup>3+</sup>/Yb<sup>3+</sup>@NaGdF<sub>4</sub>: Yb<sup>3+</sup> phosphors were investigated. After coating the NaGdF<sub>4</sub>: 0.5Er<sup>3+</sup>/0.5Tm<sup>3+</sup>/30Yb<sup>3+</sup> (0.5Er/0.5Tm/30Yb) core with NaGdF<sub>4</sub>: xYb<sup>3+</sup> (x = 25, 50) shells, the luminescence intensity of the phosphors was improved. The enhancement factor reached to 3.97 (x = 25) and 1.15 (x = 5) respectively. Owing to the different increase of up-conversion luminescence peak, the emission color changed from blue-green to yellowish-green in NaGdF<sub>4</sub>: 0.5Er<sup>3+</sup>/0.5Tm<sup>3+</sup>/30Yb<sup>3+</sup>@NaGdF<sub>4</sub>: 25Yb<sup>3+</sup> (0.5Er/0.5Tm/30Yb@25Yb) phosphors. Additionally, the temperature sensing properties of the 0.5Er/0.5Tm/30Yb@25Yb phosphors were examined. A maximum relative sensitivity (<em>S</em><sub>r</sub>) of 0.013 K<sup>−1</sup> was observed at 303 K based on florescence intensity ratio of two green emission. And a higher <em>S</em><sub>r</sub> of 0.034 K<sup>−1</sup> was recorded at 403 K based on the ratio of green (540 nm) and near-red emissions (800 nm). These findings not only deepen the understanding of the up-conversion processes in core–shell structured phosphors but also promote the development of rare-earth ion core–shell structures for applications in temperature sensing and luminescent displays.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"338 ","pages":"Article 126223"},"PeriodicalIF":4.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-11DOI: 10.1016/j.saa.2025.126222
Chenglu Zhang, Jinghao Guo, Yang Zhang, Cui Liu
Silver ions (Ag+) are widely used in industry and daily life. To achieve the specific and sensitive detection of Ag+, a novel fluorescent probe NP based on naphthalimide-phenanthroline was developed in this study. NP has an emission wavelength of 580 nm and exhibits a large Stokes shift (190 nm), showing a quenching fluorescence response to Ag+. NP can sensitively detect Ag+ with a detection limit as low as 24.6 nM. The response time is only 110 s. In particular, NP demonstrates effective detection of Ag+ in real water samples. In addition, NP exhibits low toxicity and has been successfully applied for Ag+ imaging in Hela cells.
{"title":"A novel fluorescent probe with large stokes shift based on naphthalimide and phenanthroline for specific detection of Ag+ and its application in living cells imaging","authors":"Chenglu Zhang, Jinghao Guo, Yang Zhang, Cui Liu","doi":"10.1016/j.saa.2025.126222","DOIUrl":"10.1016/j.saa.2025.126222","url":null,"abstract":"<div><div>Silver ions (Ag<sup>+</sup>) are widely used in industry and daily life. To achieve the specific and sensitive detection of Ag<sup>+</sup>, a novel fluorescent probe <strong>NP</strong> based on naphthalimide-phenanthroline was developed in this study. <strong>NP</strong> has an emission wavelength of 580 nm and exhibits a large Stokes shift (190 nm), showing a quenching fluorescence response to Ag<sup>+</sup>. <strong>NP</strong> can sensitively detect Ag<sup>+</sup> with a detection limit as low as 24.6 nM. The response time is only 110 s. In particular, <strong>NP</strong> demonstrates effective detection of Ag<sup>+</sup> in real water samples. In addition, <strong>NP</strong> exhibits low toxicity and has been successfully applied for Ag<sup>+</sup> imaging in Hela cells.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"338 ","pages":"Article 126222"},"PeriodicalIF":4.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-11DOI: 10.1016/j.saa.2025.126226
Xiaoyan Cao , Yichi Zhang , Jialing Hu , Yifan Wei , Yingyue Ding , Yanfei Kang , Chuanqing Ren , Qin Wang
Cysteine (Cys) is integral to both industrial applications and biological processes. In this study, a novel colorimetric fluorescent sensor APA, defined by its intramolecular charge transfer (ICT) properties, was optimized to effectively discriminate Cys from other structurally similar compounds such as homocysteine (Hcy) and glutathione (GSH). We present the aurone-incorporated fluorescent sensor APA, which features a 4-dimethylaminocinnamaldehyde group conjugated to the aurone scaffold and facilitates selective detection of Cys with a limit of detection (LOD) of 25.7 nM. Compared to previous studies, sensor APA exhibits near-infrared properties, a reduced reaction time of just 2 min, and a significant Stokes shift of 190 nm. Notably, APA has been successfully employed for visual imaging of Cys in test strips and quantitative detection in various food samples in real-time (including garlic, carrot, tomato, onion, green pepper, cauliflower, daikon, lotus root, apple, pear, milk powder, bread, and biscuits). Furthermore, APA has proven effective for colorimetric imaging of both endogenous and exogenous Cys in A549 cells as well as zebrafish and mice models demonstrating its practical biological applications. Overall, our findings highlight the potential of APA as one of the most promising designs for sensing Cys within the food industry and biological systems. Additionally, APA-OH serves as an ideal fluorophore for constructing fluorescence sensors aimed at bioimaging.
{"title":"Developing an aurone-based colorimetric fluorescent probe for fast cysteine sensing in foods, test strips and biological imaging","authors":"Xiaoyan Cao , Yichi Zhang , Jialing Hu , Yifan Wei , Yingyue Ding , Yanfei Kang , Chuanqing Ren , Qin Wang","doi":"10.1016/j.saa.2025.126226","DOIUrl":"10.1016/j.saa.2025.126226","url":null,"abstract":"<div><div>Cysteine (Cys) is integral to both industrial applications and biological processes. In this study, a novel colorimetric fluorescent sensor <strong>APA</strong>, defined by its intramolecular charge transfer (ICT) properties, was optimized to effectively discriminate Cys from other structurally similar compounds such as homocysteine (Hcy) and glutathione (GSH). We present the aurone-incorporated fluorescent sensor <strong>APA</strong>, which features a 4-dimethylaminocinnamaldehyde group conjugated to the aurone scaffold and facilitates selective detection of Cys with a limit of detection (LOD) of 25.7 nM. Compared to previous studies, sensor <strong>APA</strong> exhibits near-infrared properties, a reduced reaction time of just 2 min, and a significant Stokes shift of 190 nm. Notably, <strong>APA</strong> has been successfully employed for visual imaging of Cys in test strips and quantitative detection in various food samples in real-time (including garlic, carrot, tomato, onion, green pepper, cauliflower, daikon, lotus root, apple, pear, milk powder, bread, and biscuits). Furthermore, <strong>APA</strong> has proven effective for colorimetric imaging of both endogenous and exogenous Cys in A549 cells as well as zebrafish and mice models demonstrating its practical biological applications. Overall, our findings highlight the potential of <strong>APA</strong> as one of the most promising designs for sensing Cys within the food industry and biological systems. Additionally, APA-OH serves as an ideal fluorophore for constructing fluorescence sensors aimed at bioimaging.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"338 ","pages":"Article 126226"},"PeriodicalIF":4.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-11DOI: 10.1016/j.saa.2025.126224
Wen-Ru Wu , Jing Li , Yue Lin , Fang Luo , Cui-Ying Lin , Bin Qiu , Zhen-Yu Lin , Jian Wang , Fang-Zheng Huang
This study presents a smartphone-integrated portable platform for rapid lactic acid (LA) analysis using ratiometric fluorescent imaging. The system employs a dual-emission probe composed of glutathione-modified gold nanoclusters (GSH-AuNCs) and carbon quantum dots (CDs), enabling visual detection via fluorescence color transitions under 365 nm UV excitation. As LA concentration increases, the fluorescence shifts from blue to orange. Smartphone photography and RGB color analysis reveal a nonlinear sigmoidal correlation between the red-to-blue intensity ratio (R/B) and LA concentration, modeled with a coefficient of determination (R2 = 0.998). The method exhibits high selectivity and achieves recovery rates of 94.5–104.8 % in sweat samples, validated against LC-MS. This technology offers a novel, on-site solution for food quality assessment and health monitoring applications.
{"title":"A portable platform integrated with smartphones for rapid lactic acid analysis via ratiometric fluorescent imaging","authors":"Wen-Ru Wu , Jing Li , Yue Lin , Fang Luo , Cui-Ying Lin , Bin Qiu , Zhen-Yu Lin , Jian Wang , Fang-Zheng Huang","doi":"10.1016/j.saa.2025.126224","DOIUrl":"10.1016/j.saa.2025.126224","url":null,"abstract":"<div><div>This study presents a smartphone-integrated portable platform for rapid lactic acid (LA) analysis using ratiometric fluorescent imaging. The system employs a dual-emission probe composed of glutathione-modified gold nanoclusters (GSH-AuNCs) and carbon quantum dots (CDs), enabling visual detection via fluorescence color transitions under 365 nm UV excitation. As LA concentration increases, the fluorescence shifts from blue to orange. Smartphone photography and RGB color analysis reveal a nonlinear sigmoidal correlation between the red-to-blue intensity ratio (R/B) and LA concentration, modeled with a coefficient of determination (R<sup>2</sup> = 0.998). The method exhibits high selectivity and achieves recovery rates of 94.5–104.8 % in sweat samples, validated against LC-MS. This technology offers a novel, on-site solution for food quality assessment and health monitoring applications.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"338 ","pages":"Article 126224"},"PeriodicalIF":4.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-11DOI: 10.1016/j.saa.2025.126220
Dongyu Han , Nannan Yang , Xinrui Liu , Chunqiu Xia , Yoonseop Byun , Shuang Guo , Jiku Wang , Lei Chen , Young Mee Jung
Organic semiconductors are widely attracting attention as a new type of surface-enhanced Raman scattering (SERS)-active substrate. To design and fabricate high-performance SERS-active materials based on organic semiconductors, enhancing the SERS activity and exploring the SERS enhancement mechanisms are important. In this study, a sandwich structure of metal/organic semiconductor/probe molecules was fabricated via a layered self-assembly method. By changing the concentration of the poly(3-hexylthiophene) (P3HT) solution to control the thickness of the P3HT thin film, the dielectric environment surrounding the Ag nanoparticles could be changed, and the localized surface plasmon resonance phenomenon could be effectively controlled. The enhancement of the SERS performance of the Ag/P3HT composite system was studied using mercaptobenzoic acid (MBA) as a probe molecule. Our results showed that the increase in the SERS intensity tended to be stable, and the degree of charge transfer reached the maximum value at a P3HT concentration of 10−5 g/mL. Our findings also suggested that the surface plasmon resonance effect at the Ag/P3HT interface promoted transfer of electrons from the conduction band of Ag/P3HT to the lowest unoccupied molecular orbital of MBA, significantly enhancing the SERS intensity. This study, in which the detection limit for MBA molecules was as low as 10−10 mol/L, confirmed the excellent sensitivity and efficiency of the Ag/P3HT composite as a SERS substrate. The combination of the high electromagnetic field performance of Ag with the efficient charge transfer capability of P3HT significantly improved the SERS activity and detection sensitivity. This study broadens the scope of research on SERS substrates and contributes to the elucidation of the SERS mechanism.
{"title":"Concentration-Dependent SERS of P3HT in the Ag/P3HT/MBA System: A charge transfer study","authors":"Dongyu Han , Nannan Yang , Xinrui Liu , Chunqiu Xia , Yoonseop Byun , Shuang Guo , Jiku Wang , Lei Chen , Young Mee Jung","doi":"10.1016/j.saa.2025.126220","DOIUrl":"10.1016/j.saa.2025.126220","url":null,"abstract":"<div><div>Organic semiconductors are widely attracting attention as a new type of surface-enhanced Raman scattering (SERS)-active substrate. To design and fabricate high-performance SERS-active materials based on organic semiconductors, enhancing the SERS activity and exploring the SERS enhancement mechanisms are important. In this study, a sandwich structure of metal/organic semiconductor/probe molecules was fabricated via a layered self-assembly method. By changing the concentration of the poly(3-hexylthiophene) (P3HT) solution to control the thickness of the P3HT thin film, the dielectric environment surrounding the Ag nanoparticles could be changed, and the localized surface plasmon resonance phenomenon could be effectively controlled. The enhancement of the SERS performance of the Ag/P3HT composite system was studied using mercaptobenzoic acid (MBA) as a probe molecule. Our results showed that the increase in the SERS intensity tended to be stable, and the degree of charge transfer reached the maximum value at a P3HT concentration of 10<sup>−5</sup> g/mL. Our findings also suggested that the surface plasmon resonance effect at the Ag/P3HT interface promoted transfer of electrons from the conduction band of Ag/P3HT to the lowest unoccupied molecular orbital of MBA, significantly enhancing the SERS intensity. This study, in which the detection limit for MBA molecules was as low as 10<sup>−10</sup> mol/L, confirmed the excellent sensitivity and efficiency of the Ag/P3HT composite as a SERS substrate. The combination of the high electromagnetic field performance of Ag with the efficient charge transfer capability of P3HT significantly improved the SERS activity and detection sensitivity. This study broadens the scope of research on SERS substrates and contributes to the elucidation of the SERS mechanism.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"338 ","pages":"Article 126220"},"PeriodicalIF":4.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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