Pub Date : 2026-05-05Epub Date: 2026-02-02DOI: 10.1016/j.saa.2026.127550
Xiao-Hui Wang , Jun-Yu Luan , Zhi-Shi Feng , Yi-Lin Zhao , Hai-Jiao Sun , Qing-Qing Ma , Sui-Shang Zhao , Man Wang , Kun-Peng Wang , Ying Fang , Zhi-Qiang Hu
Sul-pH is a pH-sensitive fluorescent probe designed to detect mildly alkaline environments through a protonation–deprotonation mechanism. The probe is constructed using an ICT-based molecular design, with a cyclic chalcone serving as the parent fluorophore. Sul-pH exhibits a pH response range of 7–10, during which its fluorescence intensity at 601 nm increases by nearly 250-fold as the pH shifts from neutral to alkaline, accompanied by a pronounced visual color change. Moreover, the pH-responsive behavior of Sul-pH was correlated with the alkalinity generated during the hydrolysis of sulfide ions, enabling its application in the quantitative detection of S2−. The probe responds specifically to the alkaline conditions produced by S2− hydrolysis, thereby eliminating interference from HS− during sulfide detection. Impressively, Sul-pH displays a rapid response within 6 s and demonstrates excellent pH cycling stability as well as photostability. Sul-pH also shows good performance in detecting S2− in real water samples. Furthermore, Sul-pH can detect both S2− and pH in living cells, providing a reliable tool for monitoring intracellular pH dynamics and sulfide ion fluctuations within biological systems.
{"title":"A pH-responsive probe for sulfide ion detection and intracellular pH/sulfide sensing","authors":"Xiao-Hui Wang , Jun-Yu Luan , Zhi-Shi Feng , Yi-Lin Zhao , Hai-Jiao Sun , Qing-Qing Ma , Sui-Shang Zhao , Man Wang , Kun-Peng Wang , Ying Fang , Zhi-Qiang Hu","doi":"10.1016/j.saa.2026.127550","DOIUrl":"10.1016/j.saa.2026.127550","url":null,"abstract":"<div><div><strong>Sul-pH</strong> is a pH-sensitive fluorescent probe designed to detect mildly alkaline environments through a protonation–deprotonation mechanism. The probe is constructed using an ICT-based molecular design, with a cyclic chalcone serving as the parent fluorophore. <strong>Sul-pH</strong> exhibits a pH response range of 7–10, during which its fluorescence intensity at 601 nm increases by nearly 250-fold as the pH shifts from neutral to alkaline, accompanied by a pronounced visual color change. Moreover, the pH-responsive behavior of <strong>Sul-pH</strong> was correlated with the alkalinity generated during the hydrolysis of sulfide ions, enabling its application in the quantitative detection of S<sup>2−</sup>. The probe responds specifically to the alkaline conditions produced by S<sup>2−</sup> hydrolysis, thereby eliminating interference from HS<sup>−</sup> during sulfide detection. Impressively, <strong>Sul-pH</strong> displays a rapid response within 6 s and demonstrates excellent pH cycling stability as well as photostability. <strong>Sul-pH</strong> also shows good performance in detecting S<sup>2−</sup> in real water samples. Furthermore, <strong>Sul-pH</strong> can detect both S<sup>2−</sup> and pH in living cells, providing a reliable tool for monitoring intracellular pH dynamics and sulfide ion fluctuations within biological systems.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"352 ","pages":"Article 127550"},"PeriodicalIF":4.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146128090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-05Epub Date: 2026-02-02DOI: 10.1016/j.saa.2026.127549
Qi Chen, Zherui Wan, Xiaoxu Cui, Yue Hua, Guang Yang
The construction of an effective and sensitive sensing probe for tetracycline (TC) detection is meaningful and essential for the environment and food safety. Herein, an efficient ratiometric sensing platform of PDs/Eu3+ was developed through straightforward magnetic stirring of fluorescent polymer dots (PDs) and Eu3+. TC can cause blue fluorescence at 440 nm of PDs/Eu3+ to be quenched and red fluorescence emission at 617 nm increased by the internal filtration effect (IFE), static quenching and antenna effect (AE), which could be used as an ratiometric nanoprobe for the quantitative detection of TC with a lower limit of detection (LOD) of 15.1 nM. Additionally, real sample analysis of TC with higher accuracy and reliability demonstrated the practicability of the proposed sensor. Moreover, the combination of PDs/Eu3+-loaded carboxymethylcellulose (CMC) beads and smartphone offers practical value for convenient and visual detection of TC. Furthermore, owing to the stronger coordination between Al3+ and TC, the PDs/Eu3+-TC with red fluorescence emission returned to blue-green color emission after treating with Al3+. Thus a basic logic gate operation of “INHIBIT” and information storage were realized, indicating the great potential of information computing systems and displays.
{"title":"The fabrication of ratiometric fluorescence sensor for tetracycline detection based on europium-doped polymer dots","authors":"Qi Chen, Zherui Wan, Xiaoxu Cui, Yue Hua, Guang Yang","doi":"10.1016/j.saa.2026.127549","DOIUrl":"10.1016/j.saa.2026.127549","url":null,"abstract":"<div><div>The construction of an effective and sensitive sensing probe for tetracycline (TC) detection is meaningful and essential for the environment and food safety. Herein, an efficient ratiometric sensing platform of PDs/Eu<sup>3+</sup> was developed through straightforward magnetic stirring of fluorescent polymer dots (PDs) and Eu<sup>3+</sup>. TC can cause blue fluorescence at 440 nm of PDs/Eu<sup>3+</sup> to be quenched and red fluorescence emission at 617 nm increased by the internal filtration effect (IFE), static quenching and antenna effect (AE), which could be used as an ratiometric nanoprobe for the quantitative detection of TC with a lower limit of detection (LOD) of 15.1 nM. Additionally, real sample analysis of TC with higher accuracy and reliability demonstrated the practicability of the proposed sensor. Moreover, the combination of PDs/Eu<sup>3+</sup>-loaded carboxymethylcellulose (CMC) beads and smartphone offers practical value for convenient and visual detection of TC. Furthermore, owing to the stronger coordination between Al<sup>3+</sup> and TC, the PDs/Eu<sup>3+</sup>-TC with red fluorescence emission returned to blue-green color emission after treating with Al<sup>3+</sup>. Thus a basic logic gate operation of “INHIBIT” and information storage were realized, indicating the great potential of information computing systems and displays.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"352 ","pages":"Article 127549"},"PeriodicalIF":4.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146128117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-05Epub Date: 2026-01-24DOI: 10.1016/j.saa.2026.127520
Caixia Lyu , Yanliang Guo , Jiyuan Wang , Kun Tao , Jiawei Zhu
5-Hydroxymethylfurfural (HMF) is a potential food contaminant that poses health risks upon long-term intake. While current fluorescence detection technologies show potential applications in HMF analysis, most reported fluorescent probes rely on short-wavelength excitation and single-signal output, which suffer from background fluorescence interference in complex matrices. This study proposed a near-infrared excited multi-output sensing strategy based on upconversion nanoparticles (UCNPs) and p-toluidine-barbituric acid chromogenic system, enabling synergistic luminescent-colorimetric analysis of HMF. This system specifically reacted with HMF, selectively quenching the green upconversion luminescence of the UCNPs while leaving the red upconversion luminescence unaffected, constructing both ratiometric luminescent and colorimetric visual sensing modes. A smartphone-based sensing platform was further developed for portable and sensitive HMF detection, providing a more convenient and sensitive strategy for food safety analysis. This study provided a novel strategy for background-free, highly sensitive, and portable visual detection of food contaminants.
{"title":"Near-infrared excited dual-mode nanoprobes for background-free and on-site detection of 5-hydroxymethylfurfural in food","authors":"Caixia Lyu , Yanliang Guo , Jiyuan Wang , Kun Tao , Jiawei Zhu","doi":"10.1016/j.saa.2026.127520","DOIUrl":"10.1016/j.saa.2026.127520","url":null,"abstract":"<div><div>5-Hydroxymethylfurfural (HMF) is a potential food contaminant that poses health risks upon long-term intake. While current fluorescence detection technologies show potential applications in HMF analysis, most reported fluorescent probes rely on short-wavelength excitation and single-signal output, which suffer from background fluorescence interference in complex matrices. This study proposed a near-infrared excited multi-output sensing strategy based on upconversion nanoparticles (UCNPs) and p-toluidine-barbituric acid chromogenic system, enabling synergistic luminescent-colorimetric analysis of HMF. This system specifically reacted with HMF, selectively quenching the green upconversion luminescence of the UCNPs while leaving the red upconversion luminescence unaffected, constructing both ratiometric luminescent and colorimetric visual sensing modes. A smartphone-based sensing platform was further developed for portable and sensitive HMF detection, providing a more convenient and sensitive strategy for food safety analysis. This study provided a novel strategy for background-free, highly sensitive, and portable visual detection of food contaminants.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"352 ","pages":"Article 127520"},"PeriodicalIF":4.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-05Epub Date: 2026-02-05DOI: 10.1016/j.saa.2026.127564
Aleksandra Wajda , Jakub Dybas , Katarzyna Bulat , Aneta Blat , Katarzyna M. Marzec
The quality of red blood cells (RBCs) is crucial in transfusion efficacy and safety, particularly in high-risk patients. In this study, age-related biochemical alterations in stored pRBCs were investigated using a systematic, paired comparison of samples collected from pilot tubes and the main storage bags. The analyses were based on spectroscopic measurements of the isolated supernatant mixture containing RBC-derived metabolites and hemolysis products, instead of intact red blood cells. This proof-of-concept demonstrates that pilot tube samples may not reliably reflect the biochemical state of pRBCs stored in the main bag.
Our findings revealed that RBCs stored in pilot tubes undergo accelerated degradation, as indicated by elevated hemoglobin concentration, increased lactate levels, reduced glucose content, and a higher lipid-to-protein ratio. Semiquantitative analysis showed that these markers were elevated by approximately 20–100% by the seventh week of storage compared to those observed in the main blood bags. These consistent trends underscore that pilot tube samples do not reliably reflect the true biochemical quality of pRBCs intended for transfusion.
Notably, the study highlights the high diagnostic potential of FTIR and Raman spectroscopies in assessing blood quality in a rapid, non-destructive manner. These techniques offer a promising tool for point-of-care evaluation of RBC integrity directly through the storage bag, enabling improved transfusion decision-making, especially in critical care settings. By directly comparing pilot tube and main bag samples, this study reveals systematic differences in their biochemical profiles and proposes a spectroscopic framework for representative, non-invasive evaluation of pRBC quality.
{"title":"Reliability of tube-based quality assessment of packed red blood cells: Insights from FTIR and Raman spectroscopic analyses","authors":"Aleksandra Wajda , Jakub Dybas , Katarzyna Bulat , Aneta Blat , Katarzyna M. Marzec","doi":"10.1016/j.saa.2026.127564","DOIUrl":"10.1016/j.saa.2026.127564","url":null,"abstract":"<div><div>The quality of red blood cells (RBCs) is crucial in transfusion efficacy and safety, particularly in high-risk patients. In this study, age-related biochemical alterations in stored pRBCs were investigated using a systematic, paired comparison of samples collected from pilot tubes and the main storage bags. The analyses were based on spectroscopic measurements of the isolated supernatant mixture containing RBC-derived metabolites and hemolysis products, instead of intact red blood cells. This proof-of-concept demonstrates that pilot tube samples may not reliably reflect the biochemical state of pRBCs stored in the main bag.</div><div>Our findings revealed that RBCs stored in pilot tubes undergo accelerated degradation, as indicated by elevated hemoglobin concentration, increased lactate levels, reduced glucose content, and a higher lipid-to-protein ratio. Semiquantitative analysis showed that these markers were elevated by approximately 20–100% by the seventh week of storage compared to those observed in the main blood bags. These consistent trends underscore that pilot tube samples do not reliably reflect the true biochemical quality of pRBCs intended for transfusion.</div><div>Notably, the study highlights the high diagnostic potential of FTIR and Raman spectroscopies in assessing blood quality in a rapid, non-destructive manner. These techniques offer a promising tool for point-of-care evaluation of RBC integrity directly through the storage bag, enabling improved transfusion decision-making, especially in critical care settings. By directly comparing pilot tube and main bag samples, this study reveals systematic differences in their biochemical profiles and proposes a spectroscopic framework for representative, non-invasive evaluation of pRBC quality.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"352 ","pages":"Article 127564"},"PeriodicalIF":4.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146168715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ofloxacin (OFL) is a broad-spectrum and highly effective fluoroquinolone antibacterial agent. The OFL residues threaten food safety, raising public health concerns like allergic reactions and antibiotic resistance. Developing a rapid, highly sensitive detection method is crucial to mitigate these risks. This research engineered a dual-mode (fluorometric/colorimetric) immunosensing system for monitoring OFL residues. Platinum‑nickel nanoparticles (Pt–Ni NPs) characterized by a platinum-rich shell and a nickel-rich core demonstrated high oxidase-like catalytic efficiency. Pt–Ni NPs can quench the fluorescence of red carbon dots (RCDs) and facilitate the catalytic oxidation of o-phenylenediamine (OPD) to its oxidized form (oxOPD). The remaining OPD reacted with potassium permanganate (KMnO₄) to produce ratiometric colorimetry. With escalating OFL concentrations, the red fluorescence of RCDs intensified, and the yellow fluorescence of oxOPD decreased, forming a ratiometric fluorescence response. In the optimized assay regime, the detection range for OFL was 1.0 × 10−7–1.0 × 10−2 mg/mL, with a detection limit of 1.48 × 10−9 mg/mL (ratiometric fluorescence) and 1.36 × 10−8 mg/mL (ratiometric colorimetric). This method demonstrates reliable performance for the detection of OFL residuals in authentic food matrices. The dual-mode sensing strategy offers enhanced sensitivity, rapid response, and straightforward operational, making it a robust analytical tool for food safety monitoring.
{"title":"A colorimetric and ratiometric fluorescence dual-mode biosensor based on Pt–Ni bimetallic nanozymes for the detection of ofloxacin drug residues","authors":"Jinyang Li, Litao Chen, Jiayan Gao, Jianing Sun, Tongtong Li, Xue Gao","doi":"10.1016/j.saa.2026.127534","DOIUrl":"10.1016/j.saa.2026.127534","url":null,"abstract":"<div><div>Ofloxacin (OFL) is a broad-spectrum and highly effective fluoroquinolone antibacterial agent. The OFL residues threaten food safety, raising public health concerns like allergic reactions and antibiotic resistance. Developing a rapid, highly sensitive detection method is crucial to mitigate these risks. This research engineered a dual-mode (fluorometric/colorimetric) immunosensing system for monitoring OFL residues. Platinum‑nickel nanoparticles (Pt–Ni NPs) characterized by a platinum-rich shell and a nickel-rich core demonstrated high oxidase-like catalytic efficiency. Pt–Ni NPs can quench the fluorescence of red carbon dots (RCDs) and facilitate the catalytic oxidation of <em>o</em>-phenylenediamine (OPD) to its oxidized form (oxOPD). The remaining OPD reacted with potassium permanganate (KMnO₄) to produce ratiometric colorimetry. With escalating OFL concentrations, the red fluorescence of RCDs intensified, and the yellow fluorescence of oxOPD decreased, forming a ratiometric fluorescence response. In the optimized assay regime, the detection range for OFL was 1.0 × 10<sup>−7</sup>–1.0 × 10<sup>−2</sup> mg/mL, with a detection limit of 1.48 × 10<sup>−9</sup> mg/mL (ratiometric fluorescence) and 1.36 × 10<sup>−8</sup> mg/mL (ratiometric colorimetric). This method demonstrates reliable performance for the detection of OFL residuals in authentic food matrices. The dual-mode sensing strategy offers enhanced sensitivity, rapid response, and straightforward operational, making it a robust analytical tool for food safety monitoring.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"352 ","pages":"Article 127534"},"PeriodicalIF":4.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146128165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-05Epub Date: 2026-01-31DOI: 10.1016/j.saa.2026.127541
Sijia Liu , Sai Zhang , Yu Zhang , Xiaohai Yan , Kewei Feng , Yane Luo , Junfeng Yao , Tianliang Liu , Yahong Yuan , Tianli Yue , Qinglin Sheng
Staphylococcus aureus (S. aureus), a prevalent foodborne pathogen, poses a serious threat to human health, highlighting the urgent need for reliable and sensitive detection methods. Given the limitations of single-mode sensors such as false positives/negatives, a colorimetric and fluorescent dual-mode biosensor based on quantum dot material was proposed for the evaluation of S. aureus in food. This sensor utilized CsPbBr3@SiO2 quantum dots as a stable fluorescent probe. The sensing mechanism relies on the inner filter effect, where gold nanoparticles effectively quench the fluorescence of CsPbBr3@SiO2, enabling rapid and highly sensitive biosensing. Under optimized conditions, the biosensor achieved a broad detection range of 1 to 106 colony-forming unit/mL (CFU/mL), with low detection limits of 103 CFU/mL for colorimetric mode and 0.54 CFU/mL for fluorescent mode. It also exhibited excellent selectivity and was successfully applied to analyze various food samples. This study demonstrates perovskite nanomaterials as a visual tool for detecting foodborne pathogens in complex matrices.
{"title":"Colorimetric/fluorescent dual-mode biosensor for Staphylococcus aureus based on inner filter effect between AuNPs and Si/CsPbBr3 quantum dot materials","authors":"Sijia Liu , Sai Zhang , Yu Zhang , Xiaohai Yan , Kewei Feng , Yane Luo , Junfeng Yao , Tianliang Liu , Yahong Yuan , Tianli Yue , Qinglin Sheng","doi":"10.1016/j.saa.2026.127541","DOIUrl":"10.1016/j.saa.2026.127541","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> (<em>S. aureus</em>), a prevalent foodborne pathogen, poses a serious threat to human health, highlighting the urgent need for reliable and sensitive detection methods. Given the limitations of single-mode sensors such as false positives/negatives, a colorimetric and fluorescent dual-mode biosensor based on quantum dot material was proposed for the evaluation of <em>S. aureus</em> in food. This sensor utilized CsPbBr<sub>3</sub>@SiO<sub>2</sub> quantum dots as a stable fluorescent probe. The sensing mechanism relies on the inner filter effect, where gold nanoparticles effectively quench the fluorescence of CsPbBr<sub>3</sub>@SiO<sub>2</sub>, enabling rapid and highly sensitive biosensing. Under optimized conditions, the biosensor achieved a broad detection range of 1 to 10<sup>6</sup> colony-forming unit/mL (CFU/mL), with low detection limits of 103 CFU/mL for colorimetric mode and 0.54 CFU/mL for fluorescent mode. It also exhibited excellent selectivity and was successfully applied to analyze various food samples. This study demonstrates perovskite nanomaterials as a visual tool for detecting foodborne pathogens in complex matrices.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"352 ","pages":"Article 127541"},"PeriodicalIF":4.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146128073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-05Epub Date: 2026-01-24DOI: 10.1016/j.saa.2026.127519
Jie Gong , Xu-rong Liu , Xuan Liu , Ru Sun , Jian-Feng Ge
Ferroptosis constitutes a type of regulated cell death that is iron-dependent and orchestrated by lipid peroxidation. Central to the maintenance of redox homeostasis in this pathway are cysteine (Cys) and homocysteine (Hcy). To visualize the dynamics of these biothiols, three near-infrared (NIR) probes 1a-1c built upon a tetrahydroacridinium scaffold have been developed. These probes exhibited rapid responsiveness, high sensitivity, and excellent selectivity toward Cys/Hcy, accompanied by significant fluorescence enhancement and large Stokes shifts. Among them, 1a demonstrated superior mitochondria-targeting capability and low cytotoxicity, enabling real-time monitoring of Cys/Hcy from both internal cellular processes and external sources in live cells. Furthermore, 1a was utilized to monitor the depletion of mitochondrial Cys/Hcy during Erastin-induced ferroptosis and to evaluate the restorative effects of inhibitors ferrostatin-1 (Fer-1) and N-acetyl-l-cysteine (NAC), providing visual evidence of their distinct regulatory roles. Additionally, the probe could track the consumption of mitochondrial biothiols in an inflammatory model, underscoring its potential for studying redox imbalances in inflammatory processes.
{"title":"A rapid-response NIR fluorescent probe for imaging Cys/Hcy in ferroptosis and inflammation models","authors":"Jie Gong , Xu-rong Liu , Xuan Liu , Ru Sun , Jian-Feng Ge","doi":"10.1016/j.saa.2026.127519","DOIUrl":"10.1016/j.saa.2026.127519","url":null,"abstract":"<div><div>Ferroptosis constitutes a type of regulated cell death that is iron-dependent and orchestrated by lipid peroxidation. Central to the maintenance of redox homeostasis in this pathway are cysteine (Cys) and homocysteine (Hcy). To visualize the dynamics of these biothiols, three near-infrared (NIR) probes <strong>1a-1c</strong> built upon a tetrahydroacridinium scaffold have been developed. These probes exhibited rapid responsiveness, high sensitivity, and excellent selectivity toward Cys/Hcy, accompanied by significant fluorescence enhancement and large Stokes shifts. Among them, <strong>1a</strong> demonstrated superior mitochondria-targeting capability and low cytotoxicity, enabling real-time monitoring of Cys/Hcy from both internal cellular processes and external sources in live cells. Furthermore, <strong>1a</strong> was utilized to monitor the depletion of mitochondrial Cys/Hcy during Erastin-induced ferroptosis and to evaluate the restorative effects of inhibitors ferrostatin-1 (Fer-1) and <em>N</em>-acetyl-l-cysteine (NAC), providing visual evidence of their distinct regulatory roles. Additionally, the probe could track the consumption of mitochondrial biothiols in an inflammatory model, underscoring its potential for studying redox imbalances in inflammatory processes.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"352 ","pages":"Article 127519"},"PeriodicalIF":4.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-05Epub Date: 2026-01-22DOI: 10.1016/j.saa.2026.127512
Yaxuan Han , Kazuya Shimooka , Jiacheng Gao , Meng-Wan Yeh , Harumi Sato , Yukihiro Ozaki , Motohiro Tsuboi
Raman imaging offers powerful capabilities for geoscience research; however, its quantitative application to mineral–organic interactions remains underdeveloped. Building upon the work of Kitanaka et al. (2024), who combined Raman imaging with chemometric analysis for concretion studies, this research advances the approach by coupling Raman imaging with the classical least squares (CLS) method to visualize the compositional distributions within a pyrite concretion from Taiwan. Standard Raman spectroscopic analysis identified quartz, anatase, pyrite, and well-preserved organic matter as the principal constituents. By applying the CLS algorithm to hyperspectral Raman datasets, the method enables semi-quantitative determination and spatial mapping of both mineral and organic components with high precision. The resulting CLS-based Raman images reveal distinct co-localization of pyrite and kerogen within microstructures resembling biogenetic textures. These spatial patterns provide direct visual evidence that supports bacterial sulfate reduction (BSR) as a key microbial process mediating concretion growth. This study demonstrates that integrating Raman imaging with CLS modeling not only enhances quantitative interpretation of complex mineral–organic assemblages but also provides new insights into the microbially influenced mineralization processes in sedimentary environments. The proposed approach establishes a robust framework for non-destructive, semi-quantitative, and spatially resolved characterization of geobiological materials.
{"title":"Investigation of spatial distributions of components within a pyrite concretion through Raman imaging coupled with classical least squares method","authors":"Yaxuan Han , Kazuya Shimooka , Jiacheng Gao , Meng-Wan Yeh , Harumi Sato , Yukihiro Ozaki , Motohiro Tsuboi","doi":"10.1016/j.saa.2026.127512","DOIUrl":"10.1016/j.saa.2026.127512","url":null,"abstract":"<div><div>Raman imaging offers powerful capabilities for geoscience research; however, its quantitative application to mineral–organic interactions remains underdeveloped. Building upon the work of Kitanaka et al. (2024), who combined Raman imaging with chemometric analysis for concretion studies, this research advances the approach by coupling Raman imaging with the classical least squares (CLS) method to visualize the compositional distributions within a pyrite concretion from Taiwan. Standard Raman spectroscopic analysis identified quartz, anatase, pyrite, and well-preserved organic matter as the principal constituents. By applying the CLS algorithm to hyperspectral Raman datasets, the method enables semi-quantitative determination and spatial mapping of both mineral and organic components with high precision. The resulting CLS-based Raman images reveal distinct co-localization of pyrite and kerogen within microstructures resembling biogenetic textures. These spatial patterns provide direct visual evidence that supports bacterial sulfate reduction (BSR) as a key microbial process mediating concretion growth. This study demonstrates that integrating Raman imaging with CLS modeling not only enhances quantitative interpretation of complex mineral–organic assemblages but also provides new insights into the microbially influenced mineralization processes in sedimentary environments. The proposed approach establishes a robust framework for non-destructive, semi-quantitative, and spatially resolved characterization of geobiological materials.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"352 ","pages":"Article 127512"},"PeriodicalIF":4.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-05Epub Date: 2026-01-21DOI: 10.1016/j.saa.2026.127514
Guiling Peng , Qianqian Xue , Huixin Liu , Danni Chen , Qiao Liu , Siyao Zhang , Tulin Lu , Yuejun Wu , Xian Zhang , Fangzhou Yin
Scutellariae Radix (SR), a traditional herbal medicine with significant medicinal value, is widely used worldwide. To address the quality differences caused by its various commercial specifications, processing methods, and provenances, a multi-dimensional quality evaluation system was established based on content, biological potency, and spectroscopy, compensating for the inadequacy of using baicalin as sole indicator for assessing SR quality. UHPLC-Q-TOF-MS/MS technology was employed to systematically identify the chemical profiles of SR and confirm differential components among multi-source samples. The varying distribution of flavone glycosides and their aglycones in the samples was identified as the primary reason for differences in commercial specifications and processing methods. Based on this, ten marker compounds with high content and significant bioactivity were selected for quantitative analysis using UHPLC-QqQ-MS/MS. Six components are proposed as the content markers for each sample category, with corresponding limits established. Additionally, the anti-inflammatory activity of the samples was evaluated through biological potency assays, and the potency significantly increased after SR processing. To achieve real-time and efficient detection, an innovative technology combined near-infrared spectroscopy (NIR) with intelligent algorithm was developed. After preprocessing with SG+ SNV + MSC, and selecting characteristic wavelengths by SPA, the GWO-SVM model achieved 100% accuracy in sample classification. Meanwhile, after sequential optimization, the PLSR model reliably predicting the concentration of active constituents and anti-inflammatory potency. This study has accomplished a shift in quality control of SR, moving from single-component quantitative analysis to comprehensive evaluation combining active ingredient detection and bioactivity assessment, while also advancing from precise measurement to rapid predictive methods.
{"title":"Construction of a multidimensional quality evaluation system for Scutellariae Radix: Integrated research on chemical components, biological potency, near-infrared spectroscopy technology, and intelligent algorithms","authors":"Guiling Peng , Qianqian Xue , Huixin Liu , Danni Chen , Qiao Liu , Siyao Zhang , Tulin Lu , Yuejun Wu , Xian Zhang , Fangzhou Yin","doi":"10.1016/j.saa.2026.127514","DOIUrl":"10.1016/j.saa.2026.127514","url":null,"abstract":"<div><div>Scutellariae Radix (SR), a traditional herbal medicine with significant medicinal value, is widely used worldwide. To address the quality differences caused by its various commercial specifications, processing methods, and provenances, a multi-dimensional quality evaluation system was established based on content, biological potency, and spectroscopy, compensating for the inadequacy of using baicalin as sole indicator for assessing SR quality. UHPLC-Q-TOF-MS/MS technology was employed to systematically identify the chemical profiles of SR and confirm differential components among multi-source samples. The varying distribution of flavone glycosides and their aglycones in the samples was identified as the primary reason for differences in commercial specifications and processing methods. Based on this, ten marker compounds with high content and significant bioactivity were selected for quantitative analysis using UHPLC-QqQ-MS/MS. Six components are proposed as the content markers for each sample category, with corresponding limits established. Additionally, the anti-inflammatory activity of the samples was evaluated through biological potency assays, and the potency significantly increased after SR processing. To achieve real-time and efficient detection, an innovative technology combined near-infrared spectroscopy (NIR) with intelligent algorithm was developed. After preprocessing with SG+ SNV + MSC, and selecting characteristic wavelengths by SPA, the GWO-SVM model achieved 100% accuracy in sample classification. Meanwhile, after sequential optimization, the PLSR model reliably predicting the concentration of active constituents and anti-inflammatory potency. This study has accomplished a shift in quality control of SR, moving from single-component quantitative analysis to comprehensive evaluation combining active ingredient detection and bioactivity assessment, while also advancing from precise measurement to rapid predictive methods.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"352 ","pages":"Article 127514"},"PeriodicalIF":4.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-05Epub Date: 2026-02-05DOI: 10.1016/j.saa.2026.127553
Li-Wen Zhang , Zhu-Ying Yan , Xu Zhao , Li-Xia Yan , Li-Jian Chen , Xiu-Ping Yan
Bisphenol A (BPA) poses significant health risks, so its selective and sensitive determination in food is crucial. Here, we report a “turn-on” persistent luminescence aptasensor using undoped zinc gallogermanate nanoparticles (ZGGO) for selective and sensitive BPA determination in aquatic products. The sensor consists of a BPA-specific aptamer-grafted ZGGO (donor) hybridized with Cy3-labeled complementary DNA (cDNA-Cy3, acceptor). The persistent luminescence of the aptasensor is quenched via resonance energy transfer to the acceptor in the absence of BPA, and recovered in the presence of BPA due to the competitive binding of BPA with the aptamer to release cDNA-Cy3. This design effectively eliminates autofluorescence and background interference, enhancing sensitivity and selectivity. The aptasensor gives a wide linear range (1–1000 ng L−1), low detection limit (0.27 ng L−1), high selectivity, and good precision. Spike-recovery tests in fish samples yield the recovery of 85.2%–101.6%, proving its potential for monitoring BPA in complex food matrices.
双酚A （BPA）具有重大的健康风险，因此对其在食品中的选择性和灵敏度测定至关重要。在这里，我们报道了一种“开启”的持续发光传感器，使用未掺杂的没食子锗酸锌纳米颗粒（ZGGO）来选择性和敏感地测定水产品中的双酚a。该传感器由bpa特异性适配体接枝的ZGGO（供体）与cy3标记的互补DNA （cDNA-Cy3，受体）杂交组成。在没有BPA的情况下，适配体的持续发光通过向受体的共振能量转移而被猝灭，而在有BPA的情况下，由于BPA与适配体的竞争性结合释放dna - cy3而恢复发光。该设计有效地消除了自身荧光和背景干扰，提高了灵敏度和选择性。该传感器具有宽线性范围（1-1000 ng L-1），低检测限（0.27 ng L-1），高选择性和良好的精度。在鱼类样品中进行峰值回收率测试，回收率为85.2%-101.6%，证明了其监测复杂食品基质中BPA的潜力。
{"title":"Undoped zinc gallogermanate nanoparticles based persistent luminescence aptasensor for the determination of bisphenol A in aquatic products","authors":"Li-Wen Zhang , Zhu-Ying Yan , Xu Zhao , Li-Xia Yan , Li-Jian Chen , Xiu-Ping Yan","doi":"10.1016/j.saa.2026.127553","DOIUrl":"10.1016/j.saa.2026.127553","url":null,"abstract":"<div><div>Bisphenol A (BPA) poses significant health risks, so its selective and sensitive determination in food is crucial. Here, we report a “turn-on” persistent luminescence aptasensor using undoped zinc gallogermanate nanoparticles (ZGGO) for selective and sensitive BPA determination in aquatic products. The sensor consists of a BPA-specific aptamer-grafted ZGGO (donor) hybridized with Cy3-labeled complementary DNA (cDNA-Cy3, acceptor). The persistent luminescence of the aptasensor is quenched via resonance energy transfer to the acceptor in the absence of BPA, and recovered in the presence of BPA due to the competitive binding of BPA with the aptamer to release cDNA-Cy3. This design effectively eliminates autofluorescence and background interference, enhancing sensitivity and selectivity. The aptasensor gives a wide linear range (1–1000 ng L<sup>−1</sup>), low detection limit (0.27 ng L<sup>−1</sup>), high selectivity, and good precision. Spike-recovery tests in fish samples yield the recovery of 85.2%–101.6%, proving its potential for monitoring BPA in complex food matrices.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"352 ","pages":"Article 127553"},"PeriodicalIF":4.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146183858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号