Pub Date : 2022-09-01DOI: 10.35219/foodtechnology.2022.2.10
L. Dumitrașcu, Andreea Lanciu Dorofte, I. Aprodu
Spent brewer’s yeast is a by-product rich in components with biological activity, being considered an innovative and economical viable solution for creating sustainable functional food systems. This work describes the use of ultrasound treatment, performed under various conditions, with the aim to facilitate the cell lysis and to further characterize the released cellular content of the spent brewer’s yeast. Solid residue content of the spent yeast suspensions was positively influenced by both the amplitude and the exposure time (p<0.05), reaching a maximum content (40.02±0.13 g/100 g dry yeast) at 70% amplitude, after 7.5 minutes of ultrasound treatment under pulsation mode, and the minimum after 2.5 minutes of exposure at 20% ultrasound amplitude under continuous mode (31.59±1.2 g/100 g dry yeast). The content of the soluble proteins released from the yeast cells during the ultrasound treatment under pulsation mode, reached a maximum increase at amplitude of 70%, after 7.5 minutes, being by 85% higher compared to the control represented by untreated yeast suspension. The disintegration index, Z, indicated that the maximum degree of yeast cells disintegration was obtained when applying the ultrasound treatment under pulsation mode for 5 minutes, at 50% amplitude. The antioxidant activity of the yeast extracts depended on the ultrasound conditions applied, the maximum antioxidant activity being measured in the sample exposed to ultrasound under pulsation mode for 5 minutes at 50% amplitude. In conclusion, ultrasound is a good approach to release the intracellular components from the spent brewer’s yeast. Further studies factoring ultrasounds power and frequency should be conducted, such to identify the optimum set of parameters which allow the highest release of the intracellular yeast compounds.
{"title":"A preliminary study on using ultrasounds for the valorization of spent brewer’s yeast","authors":"L. Dumitrașcu, Andreea Lanciu Dorofte, I. Aprodu","doi":"10.35219/foodtechnology.2022.2.10","DOIUrl":"https://doi.org/10.35219/foodtechnology.2022.2.10","url":null,"abstract":"Spent brewer’s yeast is a by-product rich in components with biological activity, being considered an innovative and economical viable solution for creating sustainable functional food systems. This work describes the use of ultrasound treatment, performed under various conditions, with the aim to facilitate the cell lysis and to further characterize the released cellular content of the spent brewer’s yeast. Solid residue content of the spent yeast suspensions was positively influenced by both the amplitude and the exposure time (p<0.05), reaching a maximum content (40.02±0.13 g/100 g dry yeast) at 70% amplitude, after 7.5 minutes of ultrasound treatment under pulsation mode, and the minimum after 2.5 minutes of exposure at 20% ultrasound amplitude under continuous mode (31.59±1.2 g/100 g dry yeast). The content of the soluble proteins released from the yeast cells during the ultrasound treatment under pulsation mode, reached a maximum increase at amplitude of 70%, after 7.5 minutes, being by 85% higher compared to the control represented by untreated yeast suspension. The disintegration index, Z, indicated that the maximum degree of yeast cells disintegration was obtained when applying the ultrasound treatment under pulsation mode for 5 minutes, at 50% amplitude. The antioxidant activity of the yeast extracts depended on the ultrasound conditions applied, the maximum antioxidant activity being measured in the sample exposed to ultrasound under pulsation mode for 5 minutes at 50% amplitude. In conclusion, ultrasound is a good approach to release the intracellular components from the spent brewer’s yeast. Further studies factoring ultrasounds power and frequency should be conducted, such to identify the optimum set of parameters which allow the highest release of the intracellular yeast compounds.","PeriodicalId":43589,"journal":{"name":"Annals of the University Dunarea de Jos of Galati, Fascicle VI-Food Technology","volume":"147 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80627687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.35219/foodtechnology.2022.2.13
A. Ahmad, S. Sukarno, Budijanto Slamet, A. Sitanggang
Bioactive peptides showing Angiotensin-I-Converting Enzyme inhibitory (ACEi) activity can control blood pressure. They can be produced from various protein sources, including fish gelatin. This article provides a systematic review and meta-analysis on gelatin extraction, conditions of enzymatic hydrolysis, characteristics of ACEi peptides, and effect of hydrolysis procedure on ACEi peptides. Fish gelatin preparation uses various solutions such as acids, bases and distilled water. Enzymatic hydrolysis is carried out with an E/S ratio of 0.2 – 7% and temperature of 28.9 oC (using bromelain) and 60.6 oC (using Alcalase). Level of pH in hydrolysis also varied greatly, from 2 (using Pepsin) to 10 (using Purafect enzyme). The molecular weight of fish gelatin ACEi peptides ranged from 186-829 Da and the sequence of peptides was dominated by hydrophobic and aliphatic amino acids. Based on a meta-analysis, hydrolysis using enzyme combination resulted in more satisfying product than a single enzyme, represented with a combined effect size value of 0.593.
{"title":"Enzymatic hydrolysis of marine fish gelatin for producing ACE inhibitor peptides: meta-analysis","authors":"A. Ahmad, S. Sukarno, Budijanto Slamet, A. Sitanggang","doi":"10.35219/foodtechnology.2022.2.13","DOIUrl":"https://doi.org/10.35219/foodtechnology.2022.2.13","url":null,"abstract":"Bioactive peptides showing Angiotensin-I-Converting Enzyme inhibitory (ACEi) activity can control blood pressure. They can be produced from various protein sources, including fish gelatin. This article provides a systematic review and meta-analysis on gelatin extraction, conditions of enzymatic hydrolysis, characteristics of ACEi peptides, and effect of hydrolysis procedure on ACEi peptides. Fish gelatin preparation uses various solutions such as acids, bases and distilled water. Enzymatic hydrolysis is carried out with an E/S ratio of 0.2 – 7% and temperature of 28.9 oC (using bromelain) and 60.6 oC (using Alcalase). Level of pH in hydrolysis also varied greatly, from 2 (using Pepsin) to 10 (using Purafect enzyme). The molecular weight of fish gelatin ACEi peptides ranged from 186-829 Da and the sequence of peptides was dominated by hydrophobic and aliphatic amino acids. Based on a meta-analysis, hydrolysis using enzyme combination resulted in more satisfying product than a single enzyme, represented with a combined effect size value of 0.593.","PeriodicalId":43589,"journal":{"name":"Annals of the University Dunarea de Jos of Galati, Fascicle VI-Food Technology","volume":"22 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86552736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.35219/foodtechnology.2022.2.07
Wahyu Mutia Rizki, R. Dewanti-Hariyadi, H. Kusumaningrum
This study aimed to develop and compare growth models of Bacillus cereus in cooked and fried rice. A cocktail of B. cereus was inoculated into cooked and fried rice to obtain an initial concentration of ca. 2 log CFU g-1 and stored at 13, 30, 42, and 60oC. The cell concentration of B. cereus during storage were fitted to the primary models of Baranyi, Huang, and modified Gompertz. The maximum specific growth rates (µmax) were fitted to the Ratkowsky model, while the lag phase was fitted using the first-order polynomial model. There was no significant difference (p>0.05) in the µmax and lag phase of B. cereus in cooked rice or fried rice at 13 and 30oC. However, at 42oC the µmax in fried rice was significantly higher (p<0.05) and the lag phase was significantly longer (p<0.05) than those in cooked rice. No growth was observed at 60oC. The Baranyi model showed the best performance in fitting the µmax of B. cereus in cooked and fried rice. The validation study confirmed that the Ratkowsky and the first-order polynomial model could satisfactorily fit the µmax and the lag phase, respectively. Modeling of B. cereus growth in cooked and fried rice can use the primary model of Baranyi fitted to the secondary model of Ratkowsky and first-order polynomial to predict the growth rate and lag phase, respectively. The model suggests that B. cereus grows significantly faster in fried rice at 42oC.
{"title":"Comparison of predictive growth models for Bacillus cereus in cooked and fried rice during storage","authors":"Wahyu Mutia Rizki, R. Dewanti-Hariyadi, H. Kusumaningrum","doi":"10.35219/foodtechnology.2022.2.07","DOIUrl":"https://doi.org/10.35219/foodtechnology.2022.2.07","url":null,"abstract":"This study aimed to develop and compare growth models of Bacillus cereus in cooked and fried rice. A cocktail of B. cereus was inoculated into cooked and fried rice to obtain an initial concentration of ca. 2 log CFU g-1 and stored at 13, 30, 42, and 60oC. The cell concentration of B. cereus during storage were fitted to the primary models of Baranyi, Huang, and modified Gompertz. The maximum specific growth rates (µmax) were fitted to the Ratkowsky model, while the lag phase was fitted using the first-order polynomial model. There was no significant difference (p>0.05) in the µmax and lag phase of B. cereus in cooked rice or fried rice at 13 and 30oC. However, at 42oC the µmax in fried rice was significantly higher (p<0.05) and the lag phase was significantly longer (p<0.05) than those in cooked rice. No growth was observed at 60oC. The Baranyi model showed the best performance in fitting the µmax of B. cereus in cooked and fried rice. The validation study confirmed that the Ratkowsky and the first-order polynomial model could satisfactorily fit the µmax and the lag phase, respectively. Modeling of B. cereus growth in cooked and fried rice can use the primary model of Baranyi fitted to the secondary model of Ratkowsky and first-order polynomial to predict the growth rate and lag phase, respectively. The model suggests that B. cereus grows significantly faster in fried rice at 42oC.","PeriodicalId":43589,"journal":{"name":"Annals of the University Dunarea de Jos of Galati, Fascicle VI-Food Technology","volume":"38 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80343056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.35219/foodtechnology.2022.2.01
Hasifa Nanteza, R. Dewanti-Hariyadi, S. Nurjanah
This study aimed to compare the occurrence and level of Bacillus cereus sensu stricto (B. cereus) in chili, and in previously isolated white pepper from traditional and supermarkets around Bogor, to determine the phylogenetic relationship between the obtained isolates based on their 16S rDNA gene, and to determine their potential toxicity based on ces and nheA genes using Polymerase Chain Reaction. The highest presumptive B. cereus level in samples from traditional and supermarkets was 5.95×105 and 2.6×105 CFU/g respectively. The difference in B. cereus levels between the two market types was not significant. Ten presumptive isolates from chili and 10 from white pepper from our previous study were sequenced, subjected to BLAST analysis, and 13 were confirmed as B. cereus sensu lato. The sequences were phylogenetically analysed and tested for the possession of nheA and ces toxigenic genes. Based on the phylogenetic tree established, 12 of 13 isolates were related to B. cereus, sharing >98% similarity with reference strains. All 12 (100%) isolates owned the nheA gene; none of them possessed the ces gene. Absence of ces gene lessens the danger for emesis from these spices, nonetheless, the 100% presence of nheA gene presents a potential risk for B. cereus diarrheal syndrome.
{"title":"Phylogenic identification of toxigenic Bacillus cereus in chili and white pepper from Bogor area, Indonesia","authors":"Hasifa Nanteza, R. Dewanti-Hariyadi, S. Nurjanah","doi":"10.35219/foodtechnology.2022.2.01","DOIUrl":"https://doi.org/10.35219/foodtechnology.2022.2.01","url":null,"abstract":"This study aimed to compare the occurrence and level of Bacillus cereus sensu stricto (B. cereus) in chili, and in previously isolated white pepper from traditional and supermarkets around Bogor, to determine the phylogenetic relationship between the obtained isolates based on their 16S rDNA gene, and to determine their potential toxicity based on ces and nheA genes using Polymerase Chain Reaction. The highest presumptive B. cereus level in samples from traditional and supermarkets was 5.95×105 and 2.6×105 CFU/g respectively. The difference in B. cereus levels between the two market types was not significant. Ten presumptive isolates from chili and 10 from white pepper from our previous study were sequenced, subjected to BLAST analysis, and 13 were confirmed as B. cereus sensu lato. The sequences were phylogenetically analysed and tested for the possession of nheA and ces toxigenic genes. Based on the phylogenetic tree established, 12 of 13 isolates were related to B. cereus, sharing >98% similarity with reference strains. All 12 (100%) isolates owned the nheA gene; none of them possessed the ces gene. Absence of ces gene lessens the danger for emesis from these spices, nonetheless, the 100% presence of nheA gene presents a potential risk for B. cereus diarrheal syndrome.","PeriodicalId":43589,"journal":{"name":"Annals of the University Dunarea de Jos of Galati, Fascicle VI-Food Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82420228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.35219/foodtechnology.2022.2.09
I. Vasilean, Mirela Banuc, L. Patrascu, O. Constantin
The present study aimed to obtain and investigate the physicochemical and sensory characteristics of fiber-rich cereal bars containing probiotics. Different cereal bars formulations were tested, by adding Bacillus subtilis (Probisis®) cells, and/or carob powder for improving functional properties and enhancement of the color and flavor. Proximate composition, texture, color, and antioxidant activity of the cereal bars were measured. Water activity was registered below 0.5, which ensures a good microbiological stability of the products. Microbiological analyses showed that bacteria strains were viable in cereal bars after 28 days of storage at room temperature, with 8.2 to 9.5·108 CFU/g. Total phenols content and antioxidant activity were higher in carob containing cereal bars. Obtained cereal bars were well appreciated by sensory panelists, B. subtilis exerting no effect on sensory attributes. Carob powder addition was appreciated in terms of color and texture, affecting however taste and flavor.
{"title":"Studies on the use of carob powder and Bacillus subtilis for improving functionality of the cereal bars","authors":"I. Vasilean, Mirela Banuc, L. Patrascu, O. Constantin","doi":"10.35219/foodtechnology.2022.2.09","DOIUrl":"https://doi.org/10.35219/foodtechnology.2022.2.09","url":null,"abstract":"The present study aimed to obtain and investigate the physicochemical and sensory characteristics of fiber-rich cereal bars containing probiotics. Different cereal bars formulations were tested, by adding Bacillus subtilis (Probisis®) cells, and/or carob powder for improving functional properties and enhancement of the color and flavor. Proximate composition, texture, color, and antioxidant activity of the cereal bars were measured. Water activity was registered below 0.5, which ensures a good microbiological stability of the products. Microbiological analyses showed that bacteria strains were viable in cereal bars after 28 days of storage at room temperature, with 8.2 to 9.5·108 CFU/g. Total phenols content and antioxidant activity were higher in carob containing cereal bars. Obtained cereal bars were well appreciated by sensory panelists, B. subtilis exerting no effect on sensory attributes. Carob powder addition was appreciated in terms of color and texture, affecting however taste and flavor.","PeriodicalId":43589,"journal":{"name":"Annals of the University Dunarea de Jos of Galati, Fascicle VI-Food Technology","volume":"13 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91307543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.35219/foodtechnology.2022.2.11
Sadhni Induar, R. Meher, Debasmita Dubey, G. Ghosh, M. Sahu, B. Behera, P. Naik
Nature derived product drew a lot of interest because of their potential nutritional and therapeutics value. Most natural compounds are now processed and developed as possible antioxidative, antimitotic, antimicrobial, anti-inflammatory, antiangiogenic, and anti-carcinogenic pharmaceutical agents. Indeed, several natural products have been used as lead compounds in the development of most potent physiologically relevant chemicals for therapeutic application. In the current study antioxidant and anticancer potential of tuber extract of Dioscorea bulbifera have been determined as well as its antimicrobial potential against pathogenic bacteria. The IC50 value for the extract was found to be 55µg/ml for MCF-7 and 75µg/ml for MD-AMB-231 human breast cancer adenocarcinoma whereas minimum inhibitory concentration (MIC) value of the methanolic extract against S. aureus was 0.19 mg/ml and minimum bactericidal concentration (MBC) was 1.56 mg/ml. Further to identify compounds responsible for antioxidant, anticancer and antimicrobial activity GCMS analysis of the extract was conducted. Histopathological and haematological assay were carried out on animal model to identify any toxic effect of extract on vital organ system. This study may be used to provide the groundwork for the creation of novel nature-derived drugs whose biological activity can be determined by further purification, characterization, and validation of the active phytochemicals and their mechanism of action.
{"title":"Induction of apoptosis on breast adenocarcinoma and suppression of pathogenic bacterial growth by antioxidant rich tuber extract of Dioscorea Bulbifera","authors":"Sadhni Induar, R. Meher, Debasmita Dubey, G. Ghosh, M. Sahu, B. Behera, P. Naik","doi":"10.35219/foodtechnology.2022.2.11","DOIUrl":"https://doi.org/10.35219/foodtechnology.2022.2.11","url":null,"abstract":"Nature derived product drew a lot of interest because of their potential nutritional and therapeutics value. Most natural compounds are now processed and developed as possible antioxidative, antimitotic, antimicrobial, anti-inflammatory, antiangiogenic, and anti-carcinogenic pharmaceutical agents. Indeed, several natural products have been used as lead compounds in the development of most potent physiologically relevant chemicals for therapeutic application. In the current study antioxidant and anticancer potential of tuber extract of Dioscorea bulbifera have been determined as well as its antimicrobial potential against pathogenic bacteria. The IC50 value for the extract was found to be 55µg/ml for MCF-7 and 75µg/ml for MD-AMB-231 human breast cancer adenocarcinoma whereas minimum inhibitory concentration (MIC) value of the methanolic extract against S. aureus was 0.19 mg/ml and minimum bactericidal concentration (MBC) was 1.56 mg/ml. Further to identify compounds responsible for antioxidant, anticancer and antimicrobial activity GCMS analysis of the extract was conducted. Histopathological and haematological assay were carried out on animal model to identify any toxic effect of extract on vital organ system. This study may be used to provide the groundwork for the creation of novel nature-derived drugs whose biological activity can be determined by further purification, characterization, and validation of the active phytochemicals and their mechanism of action.","PeriodicalId":43589,"journal":{"name":"Annals of the University Dunarea de Jos of Galati, Fascicle VI-Food Technology","volume":"20 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75496555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Puberty represents the period in which athletes go through many changes from a physical, morpho-functional and psychological point of view. Also, in the age range between 13-14 years, the training of athletes registers a gradual increase in the volume and intensity of training, which determines special attention on the methods and means used during training. This study was carried out with the aim of identifying the level of development of the motor indices directly involved in the practice of the handball game. The research methods used during this study were: the method of analyzing specialized and interdisciplinary literature, the pedagogical observation method, the statistical-mathematical method and the tabular method. Following the study, we would like to emphasize the urgent need to update the performance scales specific to the game of handball and develop unitary training models, from the perspective of the content of the training process for each age group.
{"title":"IDENTIFICATION THE LEVEL OF DEVELOPMENT OF MOTOR INDICES AT THE LEVEL OF JUNIORS FEMALE HANDBALL TEAMS","authors":"Mădălina-Gabriela Postelnicu, C. Gheorghe","doi":"10.35219/efms.2022.1.06","DOIUrl":"https://doi.org/10.35219/efms.2022.1.06","url":null,"abstract":"Puberty represents the period in which athletes go through many changes from a physical, morpho-functional and psychological point of view. Also, in the age range between 13-14 years, the training of athletes registers a gradual increase in the volume and intensity of training, which determines special attention on the methods and means used during training. This study was carried out with the aim of identifying the level of development of the motor indices directly involved in the practice of the handball game. The research methods used during this study were: the method of analyzing specialized and interdisciplinary literature, the pedagogical observation method, the statistical-mathematical method and the tabular method. Following the study, we would like to emphasize the urgent need to update the performance scales specific to the game of handball and develop unitary training models, from the perspective of the content of the training process for each age group.","PeriodicalId":43589,"journal":{"name":"Annals of the University Dunarea de Jos of Galati, Fascicle VI-Food Technology","volume":"R-27 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84746932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction. Recommendations regarding the correctness of the therapeutic exercises must take into account the patient's body composition, which can be evaluated by bioimpedance. Material and method. 21 outpatients were assessed using a single-frequency bioelectrical impedance analyzer (SF-BIA). Health outcomes such as fat mass (FM), fat-free mass imbalances (FFM), and skeletal muscle mass (SMM) were determined. SPSS software version 25 was used for statistical analysis. Results and discussions. Of the 21 subjects, there are 52.68% men, and 47.62% women. The mean age is 47.81years ± 18.519 Std. Deviation, Body Mass Index (BMI) mean 26.38 ± 5.768, OneSample T-Test Sig..001. Fat-free mass index (FFMI), fat mass index (FMI), and skeletal mass index (SMI) were computed by adjusting with height square. Measuring the variance by ANOVA with one independent variable - BMI and one response variable (FMI Types, FFMI Types), the results were statistically significant. For FMI TypesF(2,18)=9.255, Sig.0.002, the measure of effect sizeEta Squaredη2=50.7%, Cohen medium effect shows that out of the total variation in BMI, the proportion that can be attributed to FMI Types is 50.7%. For FFMI Types F(2, 18)=10.943, Sig.0.001, the measure of effect size Eta Squaredη2=54.9%, Cohen medium effect shows that out of the total variation in BMI, the proportion that can be attributed to FFMI Types is 54.9%. FMI somatotype components results are 71.43% adipose cases, 19.05% intermediate, and 9.52% lean. One-Sample Chi-Square test applied to FMI Types reveals the statistical significance of .05(.001). FFMI somatotype components recorded 57.14% intermediate cases, 23.81% slender, and 19.05% solid. Regression equation of standard BMI and FMI with scatter plots took into consideration the “chair stand test” for pre-sarcopenia with a result of 84.5% No cases and 72.4% Yes cases.Nine patients exceeded 15 seconds at the chair stand test so probable sarcopenia was identified. Pearson correlation of BMI with FMI (r=.898), FFMI (r=.716) and SMI (r=.772), CI=99% Age (r=.518), CI=95% registered strong direct statistical significance. FMI also correlates with Age (r=.602), CI=95%, and FFMI with SMI (r=.984), CI=99%. Conclusions. Dosage of the therapeutic exercises applied with cardiac parameters monitoring for FMI Adipose (n=15), FFMI Slender, and Intermediate (n=11) includes resistive, concentric exercises, low-medium intensity progressive, pause integration for homeostasis balance, and a long period of rehabilitation for presarcopenia (n=6). For FFMI Solid, eccentric exercise can be added, medium-high intensity, pause integration for homeostasis balance for a short period with cardiac reserve monitoring. The patient's risk chart regarding fat mass and skeletal muscle mass should be included in the rehabilitation process routine to avoid functional impairment and to improve global functionality.
{"title":"DOSAGE OF THERAPEUTIC EXERCISE ACCORDING TO PATIENTS’ RISK CHART DETERMINED BY BIOIMPEDANCE","authors":"Nicolae Murgoci, C. Mereuță, Liliana Nanu","doi":"10.35219/efms.2022.1.04","DOIUrl":"https://doi.org/10.35219/efms.2022.1.04","url":null,"abstract":"Introduction. Recommendations regarding the correctness of the therapeutic exercises must take into account the patient's body composition, which can be evaluated by bioimpedance. \u0000Material and method. 21 outpatients were assessed using a single-frequency bioelectrical impedance analyzer (SF-BIA). Health outcomes such as fat mass (FM), fat-free mass imbalances (FFM), and skeletal muscle mass (SMM) were determined. SPSS software version 25 was used for statistical analysis.\u0000Results and discussions. Of the 21 subjects, there are 52.68% men, and 47.62% women. The mean age is 47.81years ± 18.519 Std. Deviation, Body Mass Index (BMI) mean 26.38 ± 5.768, OneSample T-Test Sig..001. Fat-free mass index (FFMI), fat mass index (FMI), and skeletal mass index (SMI) were computed by adjusting with height square. Measuring the variance by ANOVA with one independent variable - BMI and one response variable (FMI Types, FFMI Types), the results were statistically significant. For FMI TypesF(2,18)=9.255, Sig.0.002, the measure of effect sizeEta Squaredη2=50.7%, Cohen medium effect shows that out of the total variation in BMI, the proportion that can be attributed to FMI Types is 50.7%. For FFMI Types F(2, 18)=10.943, Sig.0.001, the measure of effect size Eta Squaredη2=54.9%, Cohen medium effect shows that out of the total variation in BMI, the proportion that can be attributed to FFMI Types is 54.9%. FMI somatotype components results are 71.43% adipose cases, 19.05% intermediate, and 9.52% lean. One-Sample Chi-Square test applied to FMI Types reveals the statistical significance of .05(.001). FFMI somatotype components recorded 57.14% intermediate cases, 23.81% slender, and 19.05% solid. Regression equation of standard BMI and FMI with scatter plots took into consideration the “chair stand test” for pre-sarcopenia with a result of 84.5% No cases and 72.4% Yes cases.Nine patients exceeded 15 seconds at the chair stand test so probable sarcopenia was identified. Pearson correlation of BMI with FMI (r=.898), FFMI (r=.716) and SMI (r=.772), CI=99% Age (r=.518), CI=95% registered strong direct statistical significance. FMI also correlates with Age (r=.602), CI=95%, and FFMI with SMI (r=.984), CI=99%.\u0000Conclusions. Dosage of the therapeutic exercises applied with cardiac parameters monitoring for FMI Adipose (n=15), FFMI Slender, and Intermediate (n=11) includes resistive, concentric exercises, low-medium intensity progressive, pause integration for homeostasis balance, and a long period of rehabilitation for presarcopenia (n=6). For FFMI Solid, eccentric exercise can be added, medium-high intensity, pause integration for homeostasis balance for a short period with cardiac reserve monitoring. The patient's risk chart regarding fat mass and skeletal muscle mass should be included in the rehabilitation process routine to avoid functional impairment and to improve global functionality.","PeriodicalId":43589,"journal":{"name":"Annals of the University Dunarea de Jos of Galati, Fascicle VI-Food Technology","volume":"196 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79898193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction. Pedaling therapy is defined as a plan of physical activity prescribed to facilitate rehabilitation from immobilizing diseases and any conditions that disrupt the movement and activity of daily life or maintain well-being through neuro-reeducation, gait training, and therapeutic activities. Material and method. It was addressed in a case study of a 40 years female patient who presented with a physiotherapy indication for gait rehabilitation from the specialist physician after immobilization in plasterboard for four weeks, the postfracture status of the lateral malleolus of the left lower limb fibula. A personalized cycling program was applied for five weeks, period March-April 2022 using a pedaling system designed and built with plantar pressure sensors attached to the pedals to monitor outputs. There was performed gait analysis, joint and muscular testing using goniometry, Borg Cycling Scale for the perception of effort and rehabilitation zone correlations, and Visual Analog Scale (VAS) for pain. The patient’s risk chart is based on the bioimpedance parameters for determining the body composition using Zepp Analyzer. Heart rate reserve, as well as heart rate training, was calculated according to Karvonen's formula supervised with Smartwatch Huawei Watch GT 2 during the cycling rehabilitation program. The sensor signals attached to the pedals are converted to analog values for both feet and the force in Newton is measured according to the values transmitted.Results and Conclusions. Rehabilitation treatment consisted of applying to the therapeutic program according to the rehabilitation phases for 5 weeks maintaining a perceptual level of 4/10 Borg (maintaining the resistance zone 2) with progressive intensity from level 3 to 5. After the rehabilitation program, average analog values between the two members were 219/240 - left / right respectively 0.89 / 0.98 N showing an insignificant force difference of 0.09 N in absolute value. The final evaluation of the joint balance by goniometry enhances exceeding the values of the functional-coefficient. Muscle balance registered an optimal value of 5/5 for the left foot. The pulse corresponding to the target range not exceeded 130. The kinetic chain deficit has improved, the muscle testing indicating a range of motion of 53 degrees in the ankle left joint versus 11 degrees after immobilization. Remission of edema after phase I (first week) proved an anti-inflammatory effect. Left foot support on the ground became possible without pain (VAS = 0). The application of the proposed pedaling program using the built horizontal device led to the achievement of the objectives regarding the facilitation of the ambulance, the system is reliable and technically functional.
{"title":"GAIT FACILITATION PROGRAM USING A HORIZONTAL BICYCLE BUILT AND DESIGNED TO MONITOR KINETIC CHAIN PARAMETERS – CASE STUDY","authors":"Nicolae Murgoci, C. Mereuță, D. Ganea","doi":"10.35219/efms.2022.1.05","DOIUrl":"https://doi.org/10.35219/efms.2022.1.05","url":null,"abstract":"Introduction. Pedaling therapy is defined as a plan of physical activity prescribed to facilitate rehabilitation from immobilizing diseases and any conditions that disrupt the movement and activity of daily life or maintain well-being through neuro-reeducation, gait training, and therapeutic activities. Material and method. It was addressed in a case study of a 40 years female patient who presented with a physiotherapy indication for gait rehabilitation from the specialist physician after immobilization in plasterboard for four weeks, the postfracture status of the lateral malleolus of the left lower limb fibula. A personalized cycling program was applied for five weeks, period March-April 2022 using a pedaling system designed and built with plantar pressure sensors attached to the pedals to monitor outputs. There was performed gait analysis, joint and muscular testing using goniometry, Borg Cycling Scale for the perception of effort and rehabilitation zone correlations, and Visual Analog Scale (VAS) for pain. The patient’s risk chart is based on the bioimpedance parameters for determining the body composition using Zepp Analyzer. Heart rate reserve, as well as heart rate training, was calculated according to Karvonen's formula supervised with Smartwatch Huawei Watch GT 2 during the cycling rehabilitation program. The sensor signals attached to the pedals are converted to analog values for both feet and the force in Newton is measured according to the values transmitted.Results and Conclusions. Rehabilitation treatment consisted of applying to the therapeutic program according to the rehabilitation phases for 5 weeks maintaining a perceptual level of 4/10 Borg (maintaining the resistance zone 2) with progressive intensity from level 3 to 5. After the rehabilitation program, average analog values between the two members were 219/240 - left / right respectively 0.89 / 0.98 N showing an insignificant force difference of 0.09 N in absolute value. The final evaluation of the joint balance by goniometry enhances exceeding the values of the functional-coefficient. Muscle balance registered an optimal value of 5/5 for the left foot. The pulse corresponding to the target range not exceeded 130. The kinetic chain deficit has improved, the muscle testing indicating a range of motion of 53 degrees in the ankle left joint versus 11 degrees after immobilization. Remission of edema after phase I (first week) proved an anti-inflammatory effect. Left foot support on the ground became possible without pain (VAS = 0). The application of the proposed pedaling program using the built horizontal device led to the achievement of the objectives regarding the facilitation of the ambulance, the system is reliable and technically functional.","PeriodicalId":43589,"journal":{"name":"Annals of the University Dunarea de Jos of Galati, Fascicle VI-Food Technology","volume":"1 6 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90804753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Gheorghe, C. Mereuță, Mădălina-Gabriela Postelnicu
The first purpose of this article was to identify the current methods to assess the anaerobic exercise capacity of athletes, both in the laboratory and in the field of sports competitions. The second purpose of the research was to highlight the factors that limit the ability of anaerobic effort, according to the literature in the field. We found that anaerobic capacity is still difficult to estimate and/or measure, mainly because of the laborious, invasive, and expensive research methodology. To ensure the quality and veracity of the data, strict adherence to test procedures, calibration of instruments, and increased attention to physiological measurements are recommended.
{"title":"METHODS FOR EVALUATING ANAEROBIC EFFORT CAPACITY OF ATHLETES","authors":"C. Gheorghe, C. Mereuță, Mădălina-Gabriela Postelnicu","doi":"10.35219/efms.2022.1.03","DOIUrl":"https://doi.org/10.35219/efms.2022.1.03","url":null,"abstract":"The first purpose of this article was to identify the current methods to assess the anaerobic exercise capacity of athletes, both in the laboratory and in the field of sports competitions. The second purpose of the research was to highlight the factors that limit the ability of anaerobic effort, according to the literature in the field. We found that anaerobic capacity is still difficult to estimate and/or measure, mainly because of the laborious, invasive, and expensive research methodology. To ensure the quality and veracity of the data, strict adherence to test procedures, calibration of instruments, and increased attention to physiological measurements are recommended.","PeriodicalId":43589,"journal":{"name":"Annals of the University Dunarea de Jos of Galati, Fascicle VI-Food Technology","volume":"72 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88535035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}