Talanta最新文献

{"title":"Aluminum-copper layered double hydroxide-enabled electrochemiluminescence-multicolor dual-mode biosensor for heparin-binding protein detection in sepsis diagnosis","authors":"Zhenjie Zheng ,&nbsp;Mengqi Ma ,&nbsp;Liang Wang ,&nbsp;Da Huang ,&nbsp;Yue Lin ,&nbsp;Jian Wang ,&nbsp;Cuiying Lin ,&nbsp;Zhenyu Lin ,&nbsp;Fang Luo","doi":"10.1016/j.talanta.2026.129458","DOIUrl":"10.1016/j.talanta.2026.129458","url":null,"abstract":"<div><div>Sepsis is a critical condition associated with substantial mortality, thus driving a pressing demand for rapid diagnostic approaches ranging from simple, readily deployable point-of-care testing (POCT) to highly sensitive laboratory-based detection methods. Heparin-binding protein (HBP), a neutrophil-derived protein, rises earlier and correlates more closely with disease severity than conventional biomarkers including procalcitonin and C-reactive protein, making it a promising biomarker for timely sepsis detection. To simultaneously meet these needs, we established a dual-mode biosensor that combines electrochemiluminescence (ECL) and multicolor for highly sensitive analysis of HBP. The sensing platform is constructed from an aluminum-copper layered double hydroxide (AlCu-LDH) scaffold conjugated with an HBP-specific aptamer and complementary DNA immobilized on magnetic beads (MBs). In the absence of HBP, the assembled AlCu-LDH-MBs probe suppresses ECL emission through ferrocene-mediated quenching while catalyzing efficient TMB oxidation to produce a strong multicolor response. Upon HBP binding, conformational switching induces probe disassembly, releasing AlCu-LDH and ferrocene from the duplex, thereby restoring ECL output while simultaneously attenuating the multicolor signal. Under optimized conditions, the biosensor achieved an ultralow detection limit of 0.023 pM in ECL mode with a linear range of 0.10 pM–10 nM, whereas the multicolor mode showed a detection limit of 0.43 nM and exhibited linearity across 0.50–10 nM, accompanied by an intuitive multicolor transition from gray to purple. Notably, this strategy avoids tedious electrode surface modification and relies on magnetic separation to regulate signal generation, thereby simplifying operation while offering a sensitive and practical tool for early sepsis diagnosis that bridges laboratory-grade analysis with instrument-free POCT.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129458"},"PeriodicalIF":6.1,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studying the capabilities of graphite furnace atomic absorption spectrometry for the discrimination of ionic copper from copper and/or copper oxide nanoparticles","authors":"Beatriz Gómez-Nieto,&nbsp;Marcela Robayo-Barragán,&nbsp;María Jesús Gismera,&nbsp;María Teresa Sevilla,&nbsp;Jesús Rodríguez Procopio","doi":"10.1016/j.talanta.2026.129459","DOIUrl":"10.1016/j.talanta.2026.129459","url":null,"abstract":"<div><div>This work explores the applicability of Graphite Furnace Atomic Absorption Spectrometry (GFAAS) for the discrimination of ionic copper (Cu²⁺) from copper and/or copper(II) oxide nanoparticles (Cu NPs and/or CuO NPs). To perform the measurements of the NPs by GFAAS, the preparation of stable suspensions from commercial solid standards of NPs with different average sizes was first evaluated. Among the evaluated dispersion media, sodium dodecyl sulphate (SDS) provided the most stable suspensions for both Cu and CuO NPs. The atomization delay (t_ad), defined as the time to reach maximum absorbance, was used as the parameter to discriminate between Cu species. Under the evaluated conditions, Cu²⁺ exhibited higher t_ad values than the nanoparticulate forms. The t_ad values remained constant for Cu concentrations ranging from 5 to 40 μg L⁻¹ for all species, demonstrating the independence of the discrimination parameter from analyte content. In addition, similar concentration calibration slopes were obtained for all species, indicating the feasibility of performing the quantification using only Cu²⁺ standards. The effects of pyrolysis and atomization temperatures, as well as the atomization heating ramp, on the resolution between the t_ad values of the Cu species were evaluated. The best time resolution for the discrimination was obtained using a pyrolysis temperature of 350 °C, a very slow atomization heating rate of 100 °C s⁻¹ and an atomization temperature of 2300 °C. Finally, the analysis of Cu²⁺ and Cu NP mixtures revealed non-additive signal profiles, suggesting possible ion - NP interactions that modify the species distribution during atomization.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"303 ","pages":"Article 129459"},"PeriodicalIF":6.1,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146090287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a near-infrared fluorescent probe for visualizing of hypochlorous acid in vitro and in vivo","authors":"Jiaoyu Wang ,&nbsp;Lu Tian ,&nbsp;Xiangli Liu ,&nbsp;Guangli Zhou ,&nbsp;Xuewei Cao ,&nbsp;Zibo Ming ,&nbsp;Zunfu Hu ,&nbsp;Yunqiang Sun ,&nbsp;Xiuwen Zheng ,&nbsp;Zhichao Dai","doi":"10.1016/j.talanta.2026.129433","DOIUrl":"10.1016/j.talanta.2026.129433","url":null,"abstract":"<div><div>Hypochlorous acid (HClO) played momentous role in various physiological processes and the aberrant expression of HClO was related to kinds of diseases including inflammation and cancer. Therefore, optical tracking of HClO was of significance for exploring its physiological and pathological effects. In this study, we designed and synthesized a new near-infrared (NIR) fluorescent probe (TMNDN) for the detection of HClO. Attributed to the robust electron-withdrawing performance of recognition moiety 2,4-dinitroaniline, the ICT process of fluorophore was interrupted and the fluorescence was switched off. Once reacted with HClO, the donor-π-acceptor (D-π-A) structure of the fluorophore was recovered, followed by the enhancement of the NIR fluorescence with a large Stokes shift. The TMNDN exhibited glorious selectivity toward HClO and was successfully applied for visualizing endogenous and exogenous HClO fluctuations in living HeLa and RAW264.7 cells, respectively. Moreover, the noninvasive visualization of endogenous HClO was further achieved in inflammatory bowel disease (IBD) and drug-induced liver injury (DILI) disease mice models <em>in vivo</em>. These above consequences demonstrated that TMNDN could acted as a reliable and promising tool for diagnosis and therapy of HClO-related diseases.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129433"},"PeriodicalIF":6.1,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress of single molecule sensors based on DNA platform","authors":"Yu Huang ,&nbsp;Muhammad Zeeshan Tahir ,&nbsp;Vesna Antic ,&nbsp;Milica Balaban ,&nbsp;Yuanwei Lin ,&nbsp;Haixia Shi ,&nbsp;Li Gao","doi":"10.1016/j.talanta.2026.129449","DOIUrl":"10.1016/j.talanta.2026.129449","url":null,"abstract":"<div><div>Deoxyribonucleic acid (DNA) is the genetic blueprint of living organisms and represents one of the most fundamental molecules across species. Recent research and development in the field of materials science indicates that DNA is not only highly programmable, but also a nanomaterial capable of precise self-assembly. Integrating diverse single-molecule detection techniques with advanced DNA nanotechnology enables direct probing of the properties of DNA as well as other molecular entities. This article aims to explain the working principles of electrical and optical detection strategies, explore system construction approaches, and evaluate their application performance. Furthermore, this review systematically summarizes the evolution of DNA-based single-molecule biosensing platforms. Electrical single-molecule sensors include single-molecule junctions (SMJs), field-effect transistors (FETs), and nanopore technologies, enabling label-free detection by monitoring electron transport, field-effect modulation, and ionic current fluctuations at the single-molecule level. Optical sensors represent another major direction and include surface-enhanced Raman scattering (SERS), fluorescence-based sensing methods, and electrochemiluminescence (ECL), enabling ultrasensitive biomolecular recognition via changes in optical signals. When DNA is employed as the recognition element, optical sensing platforms benefit from high specificity, facile programmability, and excellent signal stability. Despite remaining challenges, including stability and repeatability limitations in complex environments, integrated multimodal analysis provides a viable framework for developing robust, sensitive, and selective single-molecule sensing platforms.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129449"},"PeriodicalIF":6.1,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146045736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive colorimetric/fluorescence dual-mode sensing platform based on quasi-NENU-5 for specific detection of glyphosate","authors":"Mengjun Wang,&nbsp;Fanjie Xue,&nbsp;Miao Chen,&nbsp;Zheng Liu,&nbsp;Yi He","doi":"10.1016/j.talanta.2026.129453","DOIUrl":"10.1016/j.talanta.2026.129453","url":null,"abstract":"<div><div>Herein, a colorimetric/fluorescence dual-mode sensor was developed for ultrasensitive detection of glyphosate, utilizing a Cu-based quasi-metal-organic framework NENU-5 (QN-5) and green-emissive carbon dots (GCDs). QN-5 mimics laccase activity by catalyzing the conversion of oxygen (O₂) into superoxide radical (·O₂⁻), which then oxidizes the phenolic substrate 2,4-dichlorophenol (2,4-DP) in the presence of 4-aminoantipyrine (4-AP) to generate a distinct red quinone imine (λ_Abs = 510 nm, QI). The generated QI quenches the fluorescence of GCDs via the inner filter effect. However, the strong complexation between glyphosate and Cu²⁺ inhibits the catalytic activity of QN-5, thereby reversing the colorimetric and fluorescence signals. The developed dual-mode sensor exhibits excellent sensitivity, achieving detection limits of 11 nM in fluorescence mode and 17 nM in colorimetric mode, with recoveries ranging from 92.50 % to 102.85 %. This sensor also exhibits satisfactory selectivity and anti-interference in real samples of soybean, soil, and river water.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129453"},"PeriodicalIF":6.1,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SERS-enabled rapid detection of difenoconazole residues in tomatoes for food safety applications","authors":"Hoai Nhan Luong ,&nbsp;Le Ngoc Thu Nguyen ,&nbsp;Ngoc Bao Tri Pham ,&nbsp;Le Thai Duy ,&nbsp;Cong Khanh Tran ,&nbsp;Ngoc Phuong Nguyen ,&nbsp;Thanh Van Tran Thi ,&nbsp;Dung My Thi Dang ,&nbsp;Sutthipoj Wongrerkdee ,&nbsp;Phan Phuong Ha La ,&nbsp;Vinh Quang Dang","doi":"10.1016/j.talanta.2026.129456","DOIUrl":"10.1016/j.talanta.2026.129456","url":null,"abstract":"<div><div>Tomatoes are consumed worldwide at high volume, and difenoconazole (DFZ) is extensively applied to protect tomato crops from fungal diseases, making DFZ residue a growing food-safety concern. Surface-enhanced Raman scattering (SERS) provides a rapid and ultrasensitive approach for pesticide monitoring, but most DFZ-related studies rely solely on noble-metal electromagnetic enhancement. Integrating metals with semiconductors capable of strong charge-transfer interaction can unlock additional chemical enhancement, significantly improving low-level detection. In this work, an Ag nanoparticle-decorated α-Fe₂O₃ nanorod SERS substrate (Ag NPs/α-Fe₂O₃ NRs) is developed for rapid DFZ detection. This structure provides abundant active sites and synergistically integrates electromagnetic and chemical enhancement, enabling strong Raman signals at trace levels. The developed substrate achieves detection limits of 9.5 × 10⁻⁹ M for standard DFZ solutions and 32 μg/kg for tomato samples. The direct-growth design also improves signal uniformity and reproducibility compared with powder-based substrates. These results demonstrate that the Ag NPs/α-Fe₂O₃ nanorod SERS substrate offers a rapid, sensitive, and reliable tool for monitoring DFZ residues in tomatoes and ensuring agricultural food safety.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129456"},"PeriodicalIF":6.1,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory-compliant detection of tetracycline food contaminants: A polyindole-based chemosensoric approach for food safety monitoring and toxicological risk mitigation","authors":"Subash Jacob ,&nbsp;Mir Sahanur Ali ,&nbsp;Bhuman Gangopadhyay ,&nbsp;Sorna Lakshmi Anbu ,&nbsp;Amaya Siva ,&nbsp;Karthick Velu ,&nbsp;Souvik Pal ,&nbsp;Shamima Hussain ,&nbsp;Sasikumar Yesudass ,&nbsp;Subhenjit Hazra ,&nbsp;Amrita Pal ,&nbsp;Dipankar Chattopadhyay","doi":"10.1016/j.talanta.2026.129439","DOIUrl":"10.1016/j.talanta.2026.129439","url":null,"abstract":"<div><div>The widespread contamination of tetracycline <strong>(TC)</strong> in aquatic environments and food chains poses significant risks to human health and ecosystem sustainability, necessitating the development of rapid, sensitive detection methods. Herein, we report polyindole <strong>(PIN)</strong> fluorescent sensor for highly selective <strong>TC</strong> detection. <strong>PIN</strong> exhibited a quantum yield of 0.27 in ethanol with characteristic blue emission at 428 nm upon excitation at 350 nm. Under optimized conditions (H₂O/EtOH = 1:1, pH 7), <strong>PIN</strong> demonstrated exceptional selectivity and sensitivity toward <strong>TC</strong> over various interfering antibiotics and metal ions through fluorescence quenching. The sensor displayed a linear detection range of 0.05–7.3 μM with a remarkable limit of detection of 2.3 nM, achieving a binding constant of 1.08 × 10⁵ M⁻¹ with <strong>TC</strong>. Time-correlated single photon counting analysis revealed a static quenching mechanism governing <strong>PIN-TC</strong> interaction, while both <strong>PIN</strong> and <strong>PIN-TC</strong> complex exhibited excellent photo stability. Density functional theory calculations elucidated the molecular binding mechanism, showing strong agreement with experimental observations. Comprehensive biological evaluation using <em>Epinephelus coioides</em> eye muscle cells confirmed negligible cytotoxicity, while antimicrobial assessments against <em>Klebsiella pneumoniae</em> and <em>Staphylococcus aureus</em> demonstrated preserved antibiotic efficacy. Bio-imaging capabilities were successfully validated using <em>Daphnia pulex</em> as a model organism. The practical utility of <strong>PIN</strong> sensor was rigorously assessed across twelve diverse matrices including environmental waters, biological fluids, food beverages, and tissue samples, with recovery rates of 97.0–102.5 % validated against HPLC analysis. This work presents a versatile, cost-effective platform for TC monitoring in complex real-world samples.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129439"},"PeriodicalIF":6.1,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146045792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent probes based on 1,8-naphthalimide derivatives with large Stokes shift for dynamic monitoring of organelle interactions","authors":"Long-Kun Li ,&nbsp;Qi Lin ,&nbsp;Ru Sun ,&nbsp;Xu Xu ,&nbsp;Wen-Juan Gan ,&nbsp;Jian-Feng Ge","doi":"10.1016/j.talanta.2026.129454","DOIUrl":"10.1016/j.talanta.2026.129454","url":null,"abstract":"<div><div>Fluorescent probes with long wavelengths and large Stokes shift serve as effective tools for observing the dynamic changes of lipid droplets (LDs) and lysosomes in living cells. However, conventional 1,8-naphthalimide probes are limited by their relatively short emission wavelengths. Therefore, by combining 1,8-naphthylimide derivatives as electron acceptors with 9,10-dihydroacridine as electron donors, three polarity-responsive fluorescent probes <strong>2a-2c</strong> with twisted intramolecular charge transfer (TICT) effects were designed, specifically targeting different organelles, respectively. All three probes <strong>2a-2c</strong> exhibited significant Stokes shift (∼210 nm), long emission wavelengths (up to 601 nm), with polarity response ranges of 0 %–40 %, 0 %–70 %, and 0 %–60 % water content, respectively. As solvent polarity increased, their fluorescence intensity decreased significantly (18 − 22-fold) and emission wavelengths red-shift (71 − 95 nm). They exhibited excellent photostability, pH stability, and selectivity in complex physiological environments. Moreover, probes <strong>2a-2b</strong> could monitor real-time changes in LDs and lysosomal polarity during oxidative stress (H₂O₂) and chloroquine (CQ) treatment, respectively. Additionally, they could dynamically track organelle interactions (fusion, division, and migration) in live cells under lipopolysaccharide (LPS) or dimethyl sulfoxide (DMSO) stimulation. These results underscore the applicability of probes <strong>2a-2b</strong> for studying organelle microenvironments and their roles in disease-related processes.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129454"},"PeriodicalIF":6.1,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive lateral flow immunoassay based on the use of Au@Pt nanozyme and aptamer-captured oriented antibodies for the detection of anti-Müllerian hormone","authors":"Kseniya V. Serebrennikova,&nbsp;Nadezhda S. Komova,&nbsp;Anatoly V. Zherdev,&nbsp;Boris B. Dzantiev","doi":"10.1016/j.talanta.2026.129462","DOIUrl":"10.1016/j.talanta.2026.129462","url":null,"abstract":"<div><div>The application of core@shell nanozymes as labeling materials integrating catalytic amplification with molecular recognition in immunoassay platforms is a challenging task, since antibody immobilization often reduces both antibody reactivity and nanozyme catalytic activity. In this work, an approach to the oriented immobilization of antibodies on the surface of Au@Pt nanozymes using an aptamer specific to the fragment crystallizable region of mouse immunoglobulin G is proposed. This approach ensures that the antigen-binding domains of antibodies remain fully accessible while preserving the peroxidase-mimicking activity of the Au@Pt nanoparticles. Using oriented immobilization approach, we developed lateral flow immunoassay for sensitive detection of anti-Müllerian hormone. The sandwich immunoassay format was implemented using a pair of monoclonal antibodies that recognize different epitopes of the antigen. In this work, three approaches to conjugation, including physical adsorption, covalent and oriented immobilization to form conjugate of monoclonal antibodies with Au@Pt nanozyme, were implemented and compared in lateral flow immunoassay. The peroxidase-like properties of the nanozyme provided enhanced coloration in the test zone of the strip as a result of a catalyzed transformation with the substrate. Due to the combined effect of the high catalytic efficiency of the Au@Pt nanoparticles and the oriented immobilization of monoclonal antibodies on the surface of the nanozyme, an ultra-low detection limit of 30 pg/mL with a detection range from 0.9 to 48 ng/mL was achieved for the detection of anti-Müllerian hormone. Nanozyme-based lateral flow immunoassay allowed to reduce the detection limit by 13 times compared to common gold nanoparticle-based lateral flow immunoassay. In addition, nanozyme-based lateral flow immunoassay, including the subsequent enhancing catalytic reaction step, takes 20 min and is easy to perform. The applicability of the developed lateral flow immunoassay for the determination of anti-Müllerian hormone was confirmed by testing serum samples and revealed good recovery rates. Thus, the high analytical performance of the proposed lateral flow immunoassay demonstrates potential for anti-Müllerian hormone testing in point-of-care conditions. Moreover, the oriented immobilization of antibodies in the detection probe via the aptamer can be used as a versatile tool to improve the sensitivity of different lateral flow immunoassays.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129462"},"PeriodicalIF":6.1,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of mercury protein targets in Geobacter sulfurreducens PCA","authors":"Song Yu ,&nbsp;Guohuan Zhang ,&nbsp;Haozhong Tian ,&nbsp;Lihong Liu ,&nbsp;Bin He ,&nbsp;Ligang Hu ,&nbsp;Guibin Jiang","doi":"10.1016/j.talanta.2026.129457","DOIUrl":"10.1016/j.talanta.2026.129457","url":null,"abstract":"<div><div>Fe-reducing bacteria (FRB) play a pivotal role in regulating the biogeochemical cycling of mercury (Hg) and its associated ecological risks, while a systematic understanding of the molecular targets affected by Hg stress in these bacteria remain limited. In this study, we employed gel electrophoresis coupled with inductively coupled plasma mass spectrometry (GE-ICP-MS) to investigate the protein interaction responses to Hg stress in the model FRB <em>Geobacter sulfurreducens</em> PCA. This approach allowed us to identify Hg-binding proteins and characterize their functional implications. For the first time, we successfully resolved metalloproteins in this strain using GE-ICP-MS and identified 13 specific Hg-binding proteins, revealing critical molecular targets beyond previously known pathways. Functional analysis indicated that Hg binding to key enzymes in central carbon metabolism (<em>gapA</em>, <em>eno</em>, and <em>gltA</em>) and the ATP synthase subunit (<em>atpD</em>) induced a cellular energy deficit. Concurrently, Hg impaired protein folding (<em>groES</em> and <em>degP</em>) and synthesis machinery (<em>tuf1</em> and <em>rplI</em>), thereby disrupting the production and function of essential proteins. These processes initiate a self-limiting cascade that ultimately suppresses the Hg methylation by disrupting its essential prerequisites, which provides a mechanistic explanation for the observed fluctuation of methylation ability. These findings demonstrate the utility of GE-ICP-MS in microbial metallomics, offering​ new molecular insights into the mechanisms of microbial Hg adaptation.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129457"},"PeriodicalIF":6.1,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}