IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-05-15 Epub Date: 2026-01-21 DOI: 10.1016/j.talanta.2026.129438

Tanya Yordanova , Iva Belovezhdova , Boyan Todorov , Alina Kalyniukova , Nikolay Petkov , Vasil Andruch

In the present study, a hydrophobic deep eutectic solvent (DES) composed of lauric acid, thymol, and camphor at a molar ratio of 1:4:1 was proposed as a versatile and green extractant for ¹³⁷Cs, achieving extraction yields of 92.5 %. Bis(2-ethylhexyl) hydrogen phosphate (DEHPA) and 18-crown-6 ether were used as additives to the DES and thus boosted extraction efficiency to above 95 %. A fast and reliable procedure for the determination of ¹³⁷Cs in water samples based on liquid–liquid microextraction using a DES prior to the gamma spectrometry measurement was developed. The DES-based extraction solvent containing 10 % DEHPA could ensure excellent yields of 96 % by just vortexing for 1 min. The applicability of the proposed procedure was demonstrated by analyses of industrial water, wastewater, and dam water preliminarily spiked with ¹³⁷Cs. Recoveries ranged from 95 % to 98 %, with relative standard deviations (RSD) below 6 %, indicating the absence of significant matrix effects, as well as very good precision. Enabling 20-fold preconcentration, the limits of detection (LOD) and quantitation (LOQ) of the method were 0.40 Bq mL⁻¹ and 0.62 Bq mL⁻¹, respectively. The greenness assessment using the AGREE and ComplexMoGAPI metric tools showed that the method complies with the principles of Green Analytical Chemistry. The Click Analytical Chemistry Index (CACI) evaluated the practical utility of the method as acceptable.

在本研究中，提出了一种由月桂酸、百里香酚和樟脑组成的疏水深共晶溶剂（DES），其摩尔比为1:4:1，作为137Cs的通用绿色萃取剂，萃取率为92.5%。采用二（2-乙基己基）磷酸氢（DEHPA）和18-冠-6醚作为萃取剂，萃取率可达95%以上。建立了一种快速、可靠的测定水样中137Cs的方法，该方法基于液液微萃取，在伽马能谱测量之前使用DES。采用含10% DEHPA的des基萃取溶剂，只需旋转1 min，萃取得率可达96%。通过对工业用水、废水和大坝水进行初步加标137Cs的分析，证明了该方法的适用性。加样回收率为95% ~ 98%，相对标准偏差（RSD）小于6%，无明显基质效应，精密度高。该方法的检测限（LOD）和定量限（LOQ）分别为0.40 Bq mL−1和0.62 Bq mL−1。使用AGREE和ComplexMoGAPI度量工具进行绿色评价表明，该方法符合绿色分析化学的原则。Click分析化学指数（CACI）评估该方法的实际效用为可接受的。

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引用次数: 0

Competitive magneto enzyme-linked miRNA assay for quantification of microRNAs in exosomes 竞争性磁酶联microrna测定外泌体中microrna的定量

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-05-15 Epub Date: 2026-01-19 DOI: 10.1016/j.talanta.2026.129431

Mireia Bernuz , Mercè Martí , María Isabel Pividori

MicroRNAs (miRNAs) are non-coding, short RNA sequences involved in gene regulation. Although intracellular molecules, miRNAs are also found in biological fluids, where they serve as promising diagnostic and prognostic biomarkers. In liquid biopsies, miRNAs are protected from RNase activity through association with proteins or as part of the exosomal cargo. Current methods for miRNA detection are technically demanding and resource intensive. Here, we present a competitive magneto enzyme-linked miRNA (ELmiRNA) assay for the direct quantification of miRNAs in exosomes. The assay is based on a DNA scaffold containing two miRNA templates, each hybridized to a complementary double-tagged probe and anchored via a poly(A) tail to oligo(dT)-magnetic particles. Upon incubation with miRNA-containing samples, displacement of the tagged probes occurs, enabling indirect quantification through enzymatic labeling. As proof of concept, miRNA-21 and miRNA-27a, both upregulated in breast cancer, were quantified in MCF-7 and MDA-MB-231 cells and their derived exosomes. The assay achieved limits of detection of 1.5 nM (9.6 pg μL⁻¹) and 2.7 nM (16.2 pg μL⁻¹) for miRNA-21 and miRNA-27a, respectively, and yielded 10–30 copies per exosome, in agreement with RT-qPCR validation. The ELmiRNA assay combines sensitivity and selectivity with high-throughput analysis using a routine laboratory filter-based microplate reader. This approach represents a simplified, amplification-free alternative to RT-qPCR for miRNA quantification in exosomes, suitable for routine implementation in standard clinical and research laboratories.

MicroRNAs （miRNAs）是参与基因调控的非编码短RNA序列。虽然是细胞内分子，但mirna也存在于生物体液中，作为有希望的诊断和预后生物标志物。在液体活检中，mirna通过与蛋白质结合或作为外泌体货物的一部分而免受RNase活性的影响。目前的miRNA检测方法技术要求高，资源密集。在这里，我们提出了一种竞争性磁酶联miRNA （ELmiRNA）测定法，用于直接定量外泌体中的miRNA。该检测基于含有两个miRNA模板的DNA支架，每个模板杂交到互补的双标记探针上，并通过聚(a)尾锚定到寡核苷酸（dT）磁性颗粒上。与含有mirna的样品孵育后，标记探针发生位移，通过酶标记实现间接定量。作为概念的证明，在乳腺癌中上调的miRNA-21和miRNA-27a在MCF-7和MDA-MB-231细胞及其衍生的外泌体中被量化。miRNA-21和miRNA-27a的检测限分别为1.5 nM （9.6 pg μL−1）和2.7 nM (16.2 pg μL−1)，每个外泌体产生10-30个拷贝，与RT-qPCR验证一致。ELmiRNA分析结合了灵敏度和选择性与高通量分析使用常规实验室过滤器为基础的微孔板阅读器。该方法是一种简化的、无需扩增的替代RT-qPCR的外泌体miRNA定量方法，适用于标准临床和研究实验室的常规实施。

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引用次数: 0

Smartphone-assisted multicolor aptamer-lateral flow assay using trimetallic nanoprobes and enzyme-free signal amplification for ENR detection 使用三金属纳米探针和无酶信号放大技术进行ENR检测的智能手机辅助多色适配体横向流动分析

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-05-15 Epub Date: 2026-01-03 DOI: 10.1016/j.talanta.2025.129327

Peifang Chen , Mingwei Qin , Yu Li , Xiaodong Lu , Shuo Qi , Ali Raza , Junsong Yang , Zhouping Wang

Nanozyme-mediated lateral flow assay (LFA) remains limited by monochromatic signals, which hampers rapid visual recognition in field testing. Intelligent detection of antibiotic is essential for ensuring food safety. Here, we proposed a reliable multicolor LFA tool for detecting enrofloxacin (ENR) residues. The strategy employed aptamer-integrated catalytic hairpin assembly (CHA) to amplify molecular recognition signals. Besides, polyA-bridged DNA strands were coupled to lychee-like AuCuPt trimetallic plasmonic nanoparticles (ACPNLs) to construct cost-effective reporting probes. During result readout, the enzyme-like activity of ACPNLs regulated the etching of Au nanostars, generating structurally distinguishable multicolor changes. Combined with smartphone software, hue values as high-precision parameters were extracted from the color wheel (0–360°). The analysis revealed a robust linear relationship between ENR concentration (0.1–600 ng/mL) and colorimetric response, achieving a detection limit of 0.06 ng/mL. In contrast to single-color LFA, the multicolor platform offered an expanded hue range and enhanced visual discernibility. Overall, this method provides a new avenue for intuitive monitoring of antibiotic, showing great promise for point-of-care food safety testing.

纳米酶介导的横向流动分析（LFA）仍然受到单色信号的限制，这阻碍了现场测试中的快速视觉识别。抗生素的智能检测是确保食品安全的关键。在此，我们提出了一种可靠的多色LFA检测恩诺沙星（ENR）残留的工具。该策略采用适配体集成的催化发夹组件（CHA）来放大分子识别信号。此外，将聚a桥接DNA链偶联到荔枝样AuCuPt三金属等离子体纳米粒子（ACPNLs）上，构建了具有成本效益的报告探针。在结果读出过程中，ACPNLs的酶样活性调节Au纳米星的蚀刻，产生结构上可区分的多色变化。结合智能手机软件，从色轮（0-360°）中提取色相值作为高精度参数。分析显示，ENR浓度（0.1 ~ 600 ng/mL）与比色响应之间存在良好的线性关系，检出限为0.06 ng/mL。与单色LFA相比，多色平台提供了扩展的色调范围和增强的视觉可辨性。总的来说，该方法为抗生素的直观监测提供了新的途径，在护理点食品安全检测中显示出很大的前景。

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引用次数: 0

Intelligent optimization of cinnamon essential oil extraction: A hybrid RSM and machine learning approach for maximized yield and bioactivity 肉桂精油提取的智能优化：一种混合RSM和机器学习方法，以最大化产量和生物活性

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-05-15 Epub Date: 2026-01-06 DOI: 10.1016/j.talanta.2025.129341

Ziqi Zhu, Jingjin He, Kunying Yu, Junhui Xie, Peiyuan Li, Haiyi Zhong

To optimize cinnamon essential oil extraction and assess its potential, this study utilized response surface methodology and machine learning models. Based on single-factor experiments, these approaches were employed to predict and compare the optimal extraction process. The RSM-predicted optimal parameters included a material-to-liquid ratio of 1:10.4, distillation time of 105 min, an ultrasonic temperature of 63.0 °C, and an ultrasonic time of 48.8 min, resulting in a predicted yield of 4.61 %(actual:4.56 %). Meanwhile,the machine learning predicted parameters were a material-to liquid ratio of 1:9.3, distillation time of 90 min, an ultrasonic temperature of 55 °C, and an ultrasonic time of 40 min, yielding a predicted 4.65 % (actual: 4.60 %). machine learning demonstrated significant potential for process optimization. GC-MS analysis identified 40 components in the oil, primarily consisting of aldehydes, alkenes, and esters. The most abundant components were trans-cinnamaldehyde (19.76 %), α-copaene (16.98 %), and cinnamyl dimethyl acetal (12.92 %). Antioxidant tests showed a favorable dose-effect relationship, with over 90 % scavenging rates for ABTS•+ and DPPH•free radicals at low concentrations. Antibacterial tests revealed significant inhibition zones against Staphylococcus aureus and Escherichia coli, with minimum inhibitory concentrations of 1 mg/mL. Hemolysis tests confirmed good biocompatibility. Overall, this study elucidated the optimal extraction process, main components, and biological activities of cinnamon essential oil, indicating its potential in food preservation and medical hygiene.

为了优化肉桂精油提取工艺并评估其潜力，本研究采用响应面法和机器学习模型。在单因素实验的基础上，利用这些方法对最佳提取工艺进行预测和比较。rsm预测的最佳工艺参数为料液比为1:10 4，蒸馏时间为105 min，超声温度为63.0℃，超声时间为48.8 min，预测收率为4.61%（实际为4.56%）。同时，机器学习预测的参数为料液比为1:9.3，蒸馏时间为90 min，超声温度为55℃，超声时间为40 min，预测率为4.65%（实际为4.60%）。机器学习显示了流程优化的巨大潜力。GC-MS分析鉴定出油中的40种成分，主要由醛类、烯烃和酯类组成。其中含量最高的是反式肉桂醛（19.76%）、α-copaene（16.98%）和肉桂酰缩二甲基（12.92%）。抗氧化试验显示出良好的剂量效应关系，在低浓度下对ABTS•+和DPPH•自由基的清除率超过90%。抑菌试验显示对金黄色葡萄球菌和大肠杆菌有明显的抑制区，最低抑菌浓度为1 mg/mL。溶血试验证实具有良好的生物相容性。总之，本研究阐明了肉桂精油的最佳提取工艺、主要成分和生物活性，说明了肉桂精油在食品保鲜和医疗卫生方面的潜力。

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引用次数: 0

A ratiometric fluorescence sensor for special detection of β-galactosidase and screening of its inhibitor in complex mixtures 用于在复杂混合物中特殊检测β-半乳糖苷酶及其抑制剂筛选的比例荧光传感器

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-05-15 Epub Date: 2026-01-19 DOI: 10.1016/j.talanta.2026.129425

Jiangyue Ning , Shuangshuang Liu , Yujuan Zhan , Binyu Xiao , Hanbing Yao , Zimo Chen , Jiayi Yan , Yifu Li , Chang Shu

β-Galactosidase (β-Gal) serves as the critical biomarker for age-related disorders, and is closely related to fruit ripening and senescence. In this study, the depletion of p-nitrophenyl-β-d-galactopyranoside (PNPG) attenuated both the Förster resonance energy transfer (FRET)-mediated quantum dots (QDs) quenching and the inner-filter effects (IFE)-induced suppression of carbon dots (CDs) fluorescence, while the accumulating p-nitrophenol (PNP) conversely amplified FRET efficiency in QDs. Consequently, the ratiometric fluorescence signal (F_580nm/F_380nm) showed a positive correlation with β-Gal activity. Under optimal experimental conditions, the method demonstrated excellent linearity in the range of 5–200 U/L (R² = 0.9969), with a detection limit of 0.67 U/L. The established method was successfully applied to the high-throughput and sensitive detection of β-Gal in complex matrices (such as human serum, urine, and ripe fruits). Additionally, we identified 10 traditional Chinese medicinal herbs containing β-Gal inhibitors from 20 candidates. This study could lay the foundation for the rapid, specific and high-throughput detection of β-Gal as well as for the discovery of novel β-Gal inhibitors.

β-半乳糖苷酶（β-Gal）是年龄相关疾病的重要生物标志物，与果实成熟和衰老密切相关。在本研究中，对硝基苯基-β-d-半乙酰氨基诺苷（PNPG）的消耗减弱了Förster共振能量转移（FRET）介导的量子点（QDs）猝灭和内滤效应（IFE）诱导的碳点（CDs）荧光抑制，而对硝基酚（PNP）的积累反过来放大了量子点中的FRET效率。比值荧光信号（F580nm/F380nm）与β-Gal活性呈正相关。在最佳实验条件下，该方法在5 ~ 200 U/L范围内具有良好的线性关系（R2 = 0.9969），检出限为0.67 U/L。该方法可用于复杂基质（如人血清、尿液、成熟水果）中β-Gal的高通量、高灵敏度检测。此外，我们从20种候选药物中鉴定出10种含有β-Gal抑制剂的传统中药。本研究可为快速、特异、高通量检测β-Gal及发现新型β-Gal抑制剂奠定基础。

{"title":"A ratiometric fluorescence sensor for special detection of β-galactosidase and screening of its inhibitor in complex mixtures","authors":"Jiangyue Ning , Shuangshuang Liu , Yujuan Zhan , Binyu Xiao , Hanbing Yao , Zimo Chen , Jiayi Yan , Yifu Li , Chang Shu","doi":"10.1016/j.talanta.2026.129425","DOIUrl":"10.1016/j.talanta.2026.129425","url":null,"abstract":"<div><div>β-Galactosidase (β-Gal) serves as the critical biomarker for age-related disorders, and is closely related to fruit ripening and senescence. In this study, the depletion of <em>p</em>-nitrophenyl-β-<span>d</span>-galactopyranoside (PNPG) attenuated both the Förster resonance energy transfer (FRET)-mediated quantum dots (QDs) quenching and the inner-filter effects (IFE)-induced suppression of carbon dots (CDs) fluorescence, while the accumulating <em>p</em>-nitrophenol (PNP) conversely amplified FRET efficiency in QDs. Consequently, the ratiometric fluorescence signal (F<sub>580nm</sub>/F<sub>380nm</sub>) showed a positive correlation with β-Gal activity. Under optimal experimental conditions, the method demonstrated excellent linearity in the range of 5–200 U/L (R<sup>2</sup> = 0.9969), with a detection limit of 0.67 U/L. The established method was successfully applied to the high-throughput and sensitive detection of β-Gal in complex matrices (such as human serum, urine, and ripe fruits). Additionally, we identified 10 traditional Chinese medicinal herbs containing β-Gal inhibitors from 20 candidates. This study could lay the foundation for the rapid, specific and high-throughput detection of β-Gal as well as for the discovery of novel β-Gal inhibitors.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129425"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anchoring NiO/Ni nanoparticles onto carbon spheres for efficient electrochemical simultaneous detection of antioxidants TBHQ and BHA 锚定NiO/Ni纳米颗粒在碳球上的高效电化学同时检测抗氧化剂thbhq和BHA

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-05-15 Epub Date: 2026-01-14 DOI: 10.1016/j.talanta.2026.129419

Qing-Li Lv , Qian-Qian Xu , Qing-Qing Wang , Yu-Xin Xie , Zhong-Gang Liu , Zheng Guo

Electroanalysis as a promising method has attracted extensive attention for the detection of antioxidants tert-butylhydroquinone (TBHQ) and butylated hydroxyanisole (BHA), yet realizing their simultaneous detection remains a challenge. Herein, an electrochemical sensor based on NiO/Ni nanoparticles anchored onto carbon spheres (CSs@NiO/Ni) has been developed. Owing to synergistic effect of adsorption capacity of carbon spheres and catalytic activity of NiO/Ni nanoparticles, the proposed sensor exhibits excellent sensing performance. Moreover, a correction approach has been adopted to eliminate the interference in their simultaneous detection. The electrochemical results indicate that the simultaneous detection of TBHQ and BHA exhibited linear ranges of 0.1–60 μM, with sensitivity of 1.813 and 0.266 μA μM⁻¹, and detection limits of 0.013 and 0.038 μM, respectively. Additionally, the electrochemical sensor displays favorable repeatability, reproducibility, and anti-interference, demonstrating a reliable approach for simultaneous detection of TBHQ and BHA in complex food samples.

电分析作为一种很有前途的检测抗氧化剂叔丁基对苯二酚（TBHQ）和丁基羟基茴香醚（BHA）的方法受到了广泛的关注，但如何实现它们的同时检测仍然是一个挑战。本文开发了一种基于纳米NiO/Ni纳米颗粒锚定在碳球（CSs@NiO/Ni）上的电化学传感器。由于碳球的吸附能力和NiO/Ni纳米颗粒的催化活性的协同作用，该传感器具有优异的传感性能。此外，还采用了一种校正方法来消除两者同时检测时的干扰。电化学结果表明，同时检测TBHQ和BHA的线性范围为0.1 ~ 60 μM，灵敏度分别为1.813和0.266 μA μM−1，检出限分别为0.013和0.038 μM。此外，电化学传感器具有良好的重复性、再现性和抗干扰性，可用于同时检测复杂食品样品中的四丁基苯二酚和丁基苯二酚。

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引用次数: 0

Three pretreatment technologies for the highly sensitive electrochemical quantification of mercury in soil in the presence of interfering substances 干扰物存在下土壤中汞高灵敏度电化学定量的三种预处理技术

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-05-15 Epub Date: 2026-01-14 DOI: 10.1016/j.talanta.2026.129399

Kenshin Takemura , Wataru Iwasaki , Nobutomo Morita , Shinya Ohmagari , Kazuya Kikunaga , Yasunori Takaki , Hitomi Fukaura

To comply with current regulations on permitted levels of mercury (Hg) in soil, highly accurate and sensitive techniques are required to quantify soil Hg levels. With suitable solvent pretreatment, the electrochemical detection of Hg in the resulting soil eluates can be performed simply and with a sensitivity similar to that of expensive (e.g., mass spectrometric) methods at a fraction of their cost. However, unlike mass spectrometric analysis, which relies on columns to prepare homogeneous samples, electrochemical Hg detection suffers from the difficulty of ensuring stable measurement conditions. The preprocessing complexity of the latter method makes it unsuitable for on-site detection, and its application as an alternative to high-precision instrumental analyses remains limited. We address this problem in this study via a method for highly sensitive electrochemical Hg detection in soils, based on simple soil pretreatment involving filtration to remove large particles, cyclic interfacial electrolytic cleaning, concentration during chemical pretreatment, solution exchange after pretreatment, and electrochemical measurements. The detection limit of the developed method (0.25 ppb) meets the regulatory limit for Hg in soil.

为了遵守现行关于土壤中汞（Hg）允许含量的法规，需要高度精确和敏感的技术来量化土壤汞含量。通过适当的溶剂预处理，可以简单地进行土壤洗脱液中汞的电化学检测，并且具有与昂贵（例如质谱）方法相似的灵敏度，而成本只是其一小部分。然而，与依赖柱制备均匀样品的质谱分析不同，电化学汞检测难以确保稳定的测量条件。后一种方法的预处理复杂性使其不适合现场检测，并且其作为高精度仪器分析的替代应用仍然有限。在本研究中，我们通过一种高灵敏度的土壤汞电化学检测方法来解决这一问题，该方法基于简单的土壤预处理，包括过滤去除大颗粒、循环界面电解清洗、化学预处理期间的浓度、预处理后的溶液交换和电化学测量。该方法的检出限为0.25 ppb，符合土壤中汞的法定检出限。

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引用次数: 0

Corrigendum to "Advances in quantum dot-based fluorescence sensors for environmental and biomedical detection" [Talanta 294 (2025) 128176]. “用于环境和生物医学检测的基于量子点的荧光传感器的进展”[Talanta 294(2025) 128176]的勘误表。

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-05-15 Epub Date: 2026-01-12 DOI: 10.1016/j.talanta.2025.129270

Samaneh Ghanbarlou, Davood Kahforoushan, Hossein Abdollahi, Payam Zarrintaj, Adam Alomar, Carlos Villanueva, Seyed Mohammad Davachi

引用次数: 0

Speciation analysis reveals intracellular distribution of arsenicals among subcellular organelles of arsenic-methylated human cells exposed to arsenite. 物种形成分析揭示了暴露于亚砷酸盐的砷甲基化人细胞亚细胞器中砷的细胞内分布。

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-05-15 Epub Date: 2026-01-21 DOI: 10.1016/j.talanta.2026.129435

Yizheng Li, Hongtao Liu, Tiantian Yu, Jiahui Liu, Jin Sun, Ruijia Zhang, Na Zhao, Ligang Hu, Baowei Chen, Yongshun Huang, Tiangang Luan

Arsenic exposure via drinking water poses significant global health risks. To characterize the subcellular distributions of different arsenic species is essential for elucidating toxic effects caused by arsenic exposure. Here, arsenic-methylated L-02 cells exposed to arsenite (iAs^III) were physically partitioned into different subcellular fractions, and arsenic speciation analysis was conducted. Inorganic arsenic (iAs) and mono-methylated arsenicals (MMAs), instead of di-methylated arsenicals (DMAs), were detectable in all subcellular fractions. Regardless of subcellular fractions, iAs was the predominant species (>90.0% of the summed arsenicals). The iAs levels in different subcellular fractions were significantly correlated with total intracellular As (p < 0.01). With elevated iAs^III exposure concentration, iAs accumulation location shifted from cytoplasm to nucleus. The subcellular enrichment factors of iAs in the mitochondria and nucleus were much higher than those of MMAs. Arsenic methylation efficiency of L-02 cells was substantially reduced by high iAs^III exposure concentration. Significant correlations between methylation indices and cell viability or ATP production were observed (p < 0.05). Accordingly, mitochondrial respiration involved with ATP production was substantially suppressed by 1.0 μg/mL iAs^III. Our results suggest that methylation efficiency can be a core factor to affect intracellular arsenic distributions and induce the related cytotoxicity.

通过饮用水接触砷对全球健康构成重大风险。表征不同砷种类的亚细胞分布对阐明砷暴露引起的毒性作用至关重要。本研究将暴露于亚砷酸盐（iAsIII）的砷甲基化L-02细胞物理划分为不同的亚细胞组分，并进行砷物种形成分析。在所有亚细胞组分中检测到无机砷（iAs）和单甲基化砷（MMAs），而不是二甲基化砷（DMAs）。无论亚细胞组分如何，iAs都是优势种（占总砷的90.0%）。不同亚细胞组分的iAs水平与细胞内总暴露浓度显著相关，iAs积累位置由细胞质向细胞核转移。线粒体和细胞核内iAs的亚细胞富集因子远高于MMAs。高浓度iAsIII暴露显著降低了L-02细胞的砷甲基化效率。甲基化指数与细胞活力或ATP产生之间存在显著相关性（p III）。我们的研究结果表明，甲基化效率可能是影响细胞内砷分布和诱导相关细胞毒性的核心因素。

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引用次数: 0

Molybdenum oxide based electrode materials for electrochemical sensing applications: Recent progress in electrode modifiers 电化学传感用氧化钼基电极材料：电极改性剂的最新进展。

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-05-15 Epub Date: 2026-01-12 DOI: 10.1016/j.talanta.2026.129366

Khursheed Ahmad , Sanjeevamuthu Suganthi , Shanmugam Vignesh , Tae Hwan Oh

Recent years have observed the rapid growth in the fabrication of electrochemical sensors using metal oxides and their hybrid composites for environmental monitoring applications. It was also found that electrochemical sensors or biosensors may also use in clinical diagnostic for the determination of small biomolecules such as dopamine or ascorbic acid etc. Therefore, it is clear that electrochemical sensors have significant role for the monitoring of pollutants or small biomolecules. In this connection, molybdenum oxide (MoO₃) based electrochemical sensors were developed for the sensing of hydrogen peroxide, and glucose etc. Numerous reports are available on the use of MoO₃ or MoO₃ based composites materials for the development of electrochemical sensors. In this review article, MoO₃ and its hybrid composites with oxides, carbon-materials, sulfides, metal-organic-framework (MOF), and polymers have been reviewed for electrochemical sensing applications. We have also briefly mentioned the reported methods for the synthesis of MoO₃ and its hybrid composites. Furthermore, various electrochemical techniques have been discussed for the determination of various analytes such as hydrogen peroxide, hydroquinone, resorcinol, hydrazine, heavy metal ions, dopamine, and glucose etc. The presence of various challenges in MoO₃ based electrochemical sensors have been discussed. In addition, future trends have been mentioned for MoO₃ based electrochemical sensors. The authors of this review article believe that proposed review article will benefit the scientific community regarding the developments in the fabrication of MoO₃ based electrochemical sensors for environmental and biomedical applications.

近年来，利用金属氧化物及其混合复合材料制造的电化学传感器在环境监测中的应用迅速增长。电化学传感器或生物传感器也可用于临床诊断，如多巴胺或抗坏血酸等小生物分子的测定。因此，电化学传感器在污染物或小生物分子的监测中具有重要的作用。在此基础上，开发了基于氧化钼（MoO3）的电化学传感器，用于检测过氧化氢、葡萄糖等。关于使用MoO3或MoO3基复合材料开发电化学传感器的报道很多。本文综述了MoO3及其与氧化物、碳材料、硫化物、金属-有机框架（MOF）和聚合物的杂化复合材料在电化学传感领域的应用。本文还简要介绍了已报道的MoO3及其杂化复合材料的合成方法。本文还讨论了过氧化氢、对苯二酚、间苯二酚、肼、重金属离子、多巴胺、葡萄糖等多种分析物的电化学测定方法。讨论了基于MoO3的电化学传感器存在的各种挑战。此外，展望了基于MoO3的电化学传感器的未来发展趋势。这篇综述文章的作者认为，这篇综述文章将有利于科学界关于环境和生物医学应用中基于MoO3的电化学传感器的制造的发展。

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引用次数: 0

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