Pub Date : 2024-07-22DOI: 10.1016/j.talanta.2024.126595
Yinran Xiong, Peng Wang, Hongli Li, Jie Tang, Yuncan Chen, Lijun Zhu, Yiping Du
Multivariate calibration models often encounter challenges in extrapolating beyond the calibration instruments due to variations in hardware configurations, signal processing algorithms, or environmental conditions. Calibration transfer techniques have been developed to mitigate this issue. In this study, we introduce a novel methodology known as Supervised Factor Analysis Transfer (SFAT) aimed at achieving robust and interpretable calibration transfer. SFAT operates from a probabilistic framework and integrates response variables into its transfer process to effectively align data from the target instrument to that of the source instrument. Within the SFAT model, the data from the source instrument, the target instrument, and the response variables are collectively projected onto a shared set of latent variables. These latent variables serve as the conduit for information transfer between the three distinct domains, thereby facilitating effective spectra transfer. Moreover, SFAT explicitly models the noise variances associated with each variable, thereby minimizing the transfer of non-informative noise. Furthermore, we provide empirical evidence showcasing the efficacy of SFAT across three real-world datasets, demonstrating its superior performance in calibration transfer scenarios.
{"title":"Supervised Factor Analysis Transfer: Calibration transfer with noise modeling and response variable integration.","authors":"Yinran Xiong, Peng Wang, Hongli Li, Jie Tang, Yuncan Chen, Lijun Zhu, Yiping Du","doi":"10.1016/j.talanta.2024.126595","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126595","url":null,"abstract":"<p><p>Multivariate calibration models often encounter challenges in extrapolating beyond the calibration instruments due to variations in hardware configurations, signal processing algorithms, or environmental conditions. Calibration transfer techniques have been developed to mitigate this issue. In this study, we introduce a novel methodology known as Supervised Factor Analysis Transfer (SFAT) aimed at achieving robust and interpretable calibration transfer. SFAT operates from a probabilistic framework and integrates response variables into its transfer process to effectively align data from the target instrument to that of the source instrument. Within the SFAT model, the data from the source instrument, the target instrument, and the response variables are collectively projected onto a shared set of latent variables. These latent variables serve as the conduit for information transfer between the three distinct domains, thereby facilitating effective spectra transfer. Moreover, SFAT explicitly models the noise variances associated with each variable, thereby minimizing the transfer of non-informative noise. Furthermore, we provide empirical evidence showcasing the efficacy of SFAT across three real-world datasets, demonstrating its superior performance in calibration transfer scenarios.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A new flow batch (FB) system for chemical vapor generation (CVG) is proposed for mercury (Hg) determination in fish. An inductively coupled plasma mass spectrometer was used as a detector. Low-cost peristaltic mini pumps were used to propel the solutions and different configurations of FB systems (reactor/gas/liquid separator) were studied. The proposed configuration of the FB-CVG system allows good sensitivity, low limit of detection (LOD) and low consumption of reagents and sample solutions. In summary, only 1 mL of reductant, 1 mL of acid and 0.16 mL of sample are needed. The proposed method has good linearity, precision (better than 5 %), LOD of 0.008 μg g-1 and LOQ of 0.012 μg g-1, and high sample throughput, allowing 90 measurements/h. The accuracy of the method was evaluated through the analysis of a certified reference material (DOLT-4 Dogfish Liver), whose result is in good agreement with certified value (t-test with 95 % confidence level) and the quantification limit meets current legislations, of 1.0 μg g-1 (Brazil) and 0.3 μg g-1 (EU). In addition, analyte recovery test was done, where Hg recovery was better than 95 %, demonstrating the good analytical performance of the method. To demonstrate the applicability of the method, five samples of fish tissue (muscle) were analyzed. The proposed FB-CVG system, in addition to being low cost, is robust and requires only the volume of reagents necessary for Hg vapor generation, producing a very low amount of waste. It can be concluded that the proposed system can be used for routine analysis for Hg determination in fish tissue. It is worth noting that with the appropriate adjustments, the system can be coupled to different Hg detectors.
{"title":"A miniaturized flow batch chemical vapor generation system for Hg determination in fish by ICP-MS.","authors":"Ana Barbosa Viana, Cristiane Pappis, Cristian Kelling Pereira, Valderi Luiz Dressler","doi":"10.1016/j.talanta.2024.126600","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126600","url":null,"abstract":"<p><p>A new flow batch (FB) system for chemical vapor generation (CVG) is proposed for mercury (Hg) determination in fish. An inductively coupled plasma mass spectrometer was used as a detector. Low-cost peristaltic mini pumps were used to propel the solutions and different configurations of FB systems (reactor/gas/liquid separator) were studied. The proposed configuration of the FB-CVG system allows good sensitivity, low limit of detection (LOD) and low consumption of reagents and sample solutions. In summary, only 1 mL of reductant, 1 mL of acid and 0.16 mL of sample are needed. The proposed method has good linearity, precision (better than 5 %), LOD of 0.008 μg g<sup>-1</sup> and LOQ of 0.012 μg g<sup>-1</sup>, and high sample throughput, allowing 90 measurements/h. The accuracy of the method was evaluated through the analysis of a certified reference material (DOLT-4 Dogfish Liver), whose result is in good agreement with certified value (t-test with 95 % confidence level) and the quantification limit meets current legislations, of 1.0 μg g<sup>-1</sup> (Brazil) and 0.3 μg g<sup>-1</sup> (EU). In addition, analyte recovery test was done, where Hg recovery was better than 95 %, demonstrating the good analytical performance of the method. To demonstrate the applicability of the method, five samples of fish tissue (muscle) were analyzed. The proposed FB-CVG system, in addition to being low cost, is robust and requires only the volume of reagents necessary for Hg vapor generation, producing a very low amount of waste. It can be concluded that the proposed system can be used for routine analysis for Hg determination in fish tissue. It is worth noting that with the appropriate adjustments, the system can be coupled to different Hg detectors.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141764747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Catechol, a polyphenolic molecule and significant organic chemical intermediate, is a highly dangerous environmental contaminant due to its unpredictable nature and potential harm to both humans and the environment. This study presents the development of Sn MOF@rGO-650, identified as a hollow cube by SEM and TEM, created by carbonizing rGO on the surface of Sn MOF after in situ encapsulation. The Sn MOF@rGO-650 modified glassy carbon electrode was successfully constructed for the electrochemical detection of catechol. Under optimal conditions, the sensor exhibited a detection limit of 33 nM, a linear range of 0.20 μM-28 μM, and good long-term stability and reproducibility. This work proves for the first time that Sn MOF@rGO-650 composites can effectively detect catechol in real environmental water samples, achieving recoveries between 95.7 % and 104.8 %, and is validated in UV spectroscopy, which highlights its potential for practical applications.
儿茶酚是一种多酚分子和重要的有机化学中间体,由于其不可预测的性质以及对人类和环境的潜在危害,它是一种高度危险的环境污染物。本研究介绍了 Sn MOF@rGO-650 的开发过程,通过扫描电子显微镜(SEM)和电子显微镜(TEM)识别,该产品是通过原位封装后在 Sn MOF 表面碳化 rGO 而形成的空心立方体。成功构建的 Sn MOF@rGO-650 改性玻璃碳电极可用于儿茶酚的电化学检测。在最佳条件下,该传感器的检测限为 33 nM,线性范围为 0.20 μM-28 μM,并具有良好的长期稳定性和重现性。这项工作首次证明了 Sn MOF@rGO-650 复合材料能有效检测真实环境水样中的邻苯二酚,回收率在 95.7 % 到 104.8 % 之间,并在紫外光谱中得到了验证,突出了其实际应用的潜力。
{"title":"Electrochemical sensing platform using reduced graphene oxide and Sn MOF-derived hollow cubic composites for sensitive detection of catechol in environmental water samples.","authors":"Zhifang Liu, Jiao Li, Yang Li, Yilin Wang, Kunxiang Deng, Yixi Xie, Pengcheng Zhao, Junjie Fei","doi":"10.1016/j.talanta.2024.126602","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126602","url":null,"abstract":"<p><p>Catechol, a polyphenolic molecule and significant organic chemical intermediate, is a highly dangerous environmental contaminant due to its unpredictable nature and potential harm to both humans and the environment. This study presents the development of Sn MOF@rGO-650, identified as a hollow cube by SEM and TEM, created by carbonizing rGO on the surface of Sn MOF after in situ encapsulation. The Sn MOF@rGO-650 modified glassy carbon electrode was successfully constructed for the electrochemical detection of catechol. Under optimal conditions, the sensor exhibited a detection limit of 33 nM, a linear range of 0.20 μM-28 μM, and good long-term stability and reproducibility. This work proves for the first time that Sn MOF@rGO-650 composites can effectively detect catechol in real environmental water samples, achieving recoveries between 95.7 % and 104.8 %, and is validated in UV spectroscopy, which highlights its potential for practical applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141764758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-22DOI: 10.1016/j.talanta.2024.126603
Junjie Li, Simin Wei, Ningning Wang, Min Yang, Xinxin Xu, Jinlu Tang, Zhaohui Li
Enzyme catalytic cascade reactions based on peroxidase nanozymes and natural enzymes have aroused extensive attention in analytical fields. However, a majority of peroxidase nanozymes perform well only in acidic environments, resulting in their optimal pH mismatch with a neutral pH of natural enzymes, further restricting their application in biochemical sensing. Herein, Mn-doped CeO2 (Mn/CeO2) performing enhanced peroxidase-like activity at neutral conditions was prepared via a facile and feasible strategy. An effective enzyme cascade catalysis system via integrating glucose oxidase (GOx) with Mn/CeO2 was developed for one-pot detection of glucose in serum at neutral conditions. Using one-pot multistep catalytic reactions, this work provided a detection platform that allows for faster detection and easier operations than traditional methods. Under optimized conditions, our assay performed a sensitive detection of glucose ranging from 2.0 μΜ to 300 μΜ and a low detection limit of 0.279 μΜ. Notably, favorable analytical outcomes for glucose detection in serum samples were obtained, exhibiting potential applications in clinical diagnosis.
{"title":"The Mn-doped CeO<sub>2</sub> nanoparticles with enhanced peroxidase-like activity for one-pot analysis of glucose at neutral pH based on bio-inorganic cascade reactions.","authors":"Junjie Li, Simin Wei, Ningning Wang, Min Yang, Xinxin Xu, Jinlu Tang, Zhaohui Li","doi":"10.1016/j.talanta.2024.126603","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126603","url":null,"abstract":"<p><p>Enzyme catalytic cascade reactions based on peroxidase nanozymes and natural enzymes have aroused extensive attention in analytical fields. However, a majority of peroxidase nanozymes perform well only in acidic environments, resulting in their optimal pH mismatch with a neutral pH of natural enzymes, further restricting their application in biochemical sensing. Herein, Mn-doped CeO<sub>2</sub> (Mn/CeO<sub>2</sub>) performing enhanced peroxidase-like activity at neutral conditions was prepared via a facile and feasible strategy. An effective enzyme cascade catalysis system via integrating glucose oxidase (GOx) with Mn/CeO<sub>2</sub> was developed for one-pot detection of glucose in serum at neutral conditions. Using one-pot multistep catalytic reactions, this work provided a detection platform that allows for faster detection and easier operations than traditional methods. Under optimized conditions, our assay performed a sensitive detection of glucose ranging from 2.0 μΜ to 300 μΜ and a low detection limit of 0.279 μΜ. Notably, favorable analytical outcomes for glucose detection in serum samples were obtained, exhibiting potential applications in clinical diagnosis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-20DOI: 10.1016/j.talanta.2024.126594
Yi-Ming Zhao, Ying-Na Li, Rui Ma, Chun-Lei Ji, Yan-Hong Mu, Rong Xu, Li-Wei Sun, Fang-Bing Liu
Ginseng, a highly esteemed herbal medicine, has been utilized over 5000 years, predominantly in Far Eastern countries. Ginseng is categorized into garden ginseng (GG) and ginseng under forest (FG). However, in contrast to FG, excessive intake of GG may lead to potential adverse effects due to disruption of epithelial cell integrity, and the specific population groups that may be at higher risk. In this work, untargeted metabolomics were used to determine the heterogeneity between GG and FG, the data indicates that the content of Ethyl caffeate, Homoorientin, Citric acid and Quinic acid in GG were higher than in FG. Mass spectrometry imaging showed that ethyl caffeate and Homoorientin were concentrated on the brownish yellow exocarp of the primary root. Our experiments demonstrated that excessive exposure to ethyl caffeate and Homoorientin exacerbated the inflammatory response of HUVECs and reduced the expression of cell junctions. This suggest that the compounds causing adverse effects from excessive intake of GG are mainly concentrated in the yellow exocarp of the primary root of GG. These results suggest that untargeted metabolomics coupled with MALDI-MSI can visualize the spatial distribution of endogenous differential molecules of the same herb in different growth environments or developmental stages.
{"title":"Matrix-assisted laser desorption ionization mass spectrometry imaging reveals the spatial distribution of compounds that may exacerbate inflammation in garden ginseng and ginseng under forest.","authors":"Yi-Ming Zhao, Ying-Na Li, Rui Ma, Chun-Lei Ji, Yan-Hong Mu, Rong Xu, Li-Wei Sun, Fang-Bing Liu","doi":"10.1016/j.talanta.2024.126594","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126594","url":null,"abstract":"<p><p>Ginseng, a highly esteemed herbal medicine, has been utilized over 5000 years, predominantly in Far Eastern countries. Ginseng is categorized into garden ginseng (GG) and ginseng under forest (FG). However, in contrast to FG, excessive intake of GG may lead to potential adverse effects due to disruption of epithelial cell integrity, and the specific population groups that may be at higher risk. In this work, untargeted metabolomics were used to determine the heterogeneity between GG and FG, the data indicates that the content of Ethyl caffeate, Homoorientin, Citric acid and Quinic acid in GG were higher than in FG. Mass spectrometry imaging showed that ethyl caffeate and Homoorientin were concentrated on the brownish yellow exocarp of the primary root. Our experiments demonstrated that excessive exposure to ethyl caffeate and Homoorientin exacerbated the inflammatory response of HUVECs and reduced the expression of cell junctions. This suggest that the compounds causing adverse effects from excessive intake of GG are mainly concentrated in the yellow exocarp of the primary root of GG. These results suggest that untargeted metabolomics coupled with MALDI-MSI can visualize the spatial distribution of endogenous differential molecules of the same herb in different growth environments or developmental stages.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-20DOI: 10.1016/j.talanta.2024.126579
Kritika S Sharma, Kajal Panchal, Dinesh Kumar
There is great interest in fabricating devices that can detect and remove water pollutants, especially heavy metal ions and dyes from wastewater, to promote sustainable water use. In this study, an extract of Borassus flabellifer leaves (BoF-LE) was used to synthesize silver nanoparticles (BoF-AgNPs), with the BoF-LE serving as a reducing and capping agent. The sensitivity and selectivity of BoF-AgNPs for Mn(II) ions were tested by comparing with the control sample and other competent metal ions. Our results showed that BoF-AgNPs are extremely sensitive and selective in detecting Mn(II) ions, with a detection limit of 0.3 ppb. HR-TEM, UV-Vis spectroscopy, and DLS investigations were used to confirm that BoF-AgNPs detect Mn(II) ions by an aggregation-based mechanism. Additionally, it was found that BoF-AgNPs are effective in rapidly decolorizing MB dye, as demonstrated by their ability to decolorize MB by 92.66% within 7 min. This study is the first to report successful synthesis of BoF-AgNPs and their two applications, which are enabled with an Inhibit-AND logic gate. Using BoF-AgNPs to detect and degrade water pollutants may promote sustainable water use.
{"title":"Inhibit-AND logic gate enabled versatile BoF-AgNPs as ultrasensitive and selective nanoprobe for Mn(II) ions and nanocatalyst for rapid MB decoloration.","authors":"Kritika S Sharma, Kajal Panchal, Dinesh Kumar","doi":"10.1016/j.talanta.2024.126579","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126579","url":null,"abstract":"<p><p>There is great interest in fabricating devices that can detect and remove water pollutants, especially heavy metal ions and dyes from wastewater, to promote sustainable water use. In this study, an extract of Borassus flabellifer leaves (BoF-LE) was used to synthesize silver nanoparticles (BoF-AgNPs), with the BoF-LE serving as a reducing and capping agent. The sensitivity and selectivity of BoF-AgNPs for Mn(II) ions were tested by comparing with the control sample and other competent metal ions. Our results showed that BoF-AgNPs are extremely sensitive and selective in detecting Mn(II) ions, with a detection limit of 0.3 ppb. HR-TEM, UV-Vis spectroscopy, and DLS investigations were used to confirm that BoF-AgNPs detect Mn(II) ions by an aggregation-based mechanism. Additionally, it was found that BoF-AgNPs are effective in rapidly decolorizing MB dye, as demonstrated by their ability to decolorize MB by 92.66% within 7 min. This study is the first to report successful synthesis of BoF-AgNPs and their two applications, which are enabled with an Inhibit-AND logic gate. Using BoF-AgNPs to detect and degrade water pollutants may promote sustainable water use.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Recently, peptide-drug conjugate (PDC) has become the most promising conjugated drug for tumor therapy after antibody-drug conjugate due to stronger tumor penetration capacity and lower immunogenicity. CBP-1018 was a PDC with dual-ligand conjugated to MMAE via a cleavable linker (MC-Val-Cit-PABC) that can be lysed by cathepsins B. In this study, two specific LC-MS/MS methods were developed and validated for the determination of CBP-1018 and its metabolite MMAE in human plasma. To prevent the cleavable MC-Val-Cit-PABC linker from degradation, a protease inhibitor (cOmplete solution) was added to the pre-cooled vacuum tubes and the separated plasma samples. The assays involved the pretreatment of CBP-1018 by protein precipitation with H2O-ACN (1:9, v/v) and the extraction of MMAE by liquid-liquid extraction with ethyl acetate under alkaline condition to eliminate the interference of CBP-1018 on MMAE. The two analytes showed good linearities over the calibration ranges (R2 ≥ 9980). Both accuracy and precision met the acceptance criteria. The validated methods were successfully applied to the phase I dose-escalation study of CBP-1018 injection in Chinese patients with solid tumors to evaluate the pharmacokinetic properties of CBP-1018 and MMAE. The results showed that CBP-1018 was eliminated immediately after injection and MMAE reached the maximum exposure at approximately 2 h after infusion. The maximum concentration of MMAE did not exceed 20.0 ng/mL, suggesting that the off-target toxicity of CBP-1018 injection was controllable.
{"title":"LC-MS/MS method for dual-ligand peptide-drug CBP-1018 and its deconjugated payload MMAE including sample stabilization strategy for its MC-Val-Cit-PABC linker.","authors":"Xianjing Li, Minlu Cheng, Yiya Wang, Chang Shu, Bingjie Zou, Qinxin Song, Li Ding","doi":"10.1016/j.talanta.2024.126596","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126596","url":null,"abstract":"<p><p>Recently, peptide-drug conjugate (PDC) has become the most promising conjugated drug for tumor therapy after antibody-drug conjugate due to stronger tumor penetration capacity and lower immunogenicity. CBP-1018 was a PDC with dual-ligand conjugated to MMAE via a cleavable linker (MC-Val-Cit-PABC) that can be lysed by cathepsins B. In this study, two specific LC-MS/MS methods were developed and validated for the determination of CBP-1018 and its metabolite MMAE in human plasma. To prevent the cleavable MC-Val-Cit-PABC linker from degradation, a protease inhibitor (cOmplete solution) was added to the pre-cooled vacuum tubes and the separated plasma samples. The assays involved the pretreatment of CBP-1018 by protein precipitation with H<sub>2</sub>O-ACN (1:9, v/v) and the extraction of MMAE by liquid-liquid extraction with ethyl acetate under alkaline condition to eliminate the interference of CBP-1018 on MMAE. The two analytes showed good linearities over the calibration ranges (R<sup>2</sup> ≥ 9980). Both accuracy and precision met the acceptance criteria. The validated methods were successfully applied to the phase I dose-escalation study of CBP-1018 injection in Chinese patients with solid tumors to evaluate the pharmacokinetic properties of CBP-1018 and MMAE. The results showed that CBP-1018 was eliminated immediately after injection and MMAE reached the maximum exposure at approximately 2 h after infusion. The maximum concentration of MMAE did not exceed 20.0 ng/mL, suggesting that the off-target toxicity of CBP-1018 injection was controllable.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Avian influenza viruses (AIV) are capable of infecting a considerable proportion of the world's population each year, leading to severe epidemics with high rates of morbidity and mortality. The methods now used to diagnose influenza virus A include the Western blot test (WB), hemagglutination inhibition (HI), and enzyme-linked immunosorbent assays (ELISAs). But because of their labor-intensiveness, lengthy procedures, need for costly equipment, and inexperienced staff, these approaches are considered inappropriate. The present review elucidates the recent advancements in the field of avian influenza detection through the utilization of nanomaterials-based immunosensors between 2014 and 2024. The classification of detection techniques has been taken into account to provide a comprehensive overview of the literature. The review encompasses a detailed illustration of the commonly employed detection mechanisms in immunosensors, namely, colorimetry, fluorescence assay, surface plasmon resonance (SPR), surface-enhanced Raman spectroscopy (SERS), electrochemical detection, quartz crystal microbalance (QCM) piezoelectric, and field-effect transistor (FET). Furthermore, the challenges and future prospects for the immunosensors have been deliberated upon. The present review aims to enhance the understanding of immunosensors-based sensing platforms for virus detection and to stimulate the development of novel immunosensors by providing novel ideas and inspirations. Therefore, the aim of this paper is to provide an updated information about biosensors, as a recent detection technique of influenza with its details regarding the various types of biosensors, which can be used for this review.
{"title":"Nanomaterials-based immunosensors for avian influenza virus detection.","authors":"Fariba Mollarasouli, Sonia Bahrani, Yousef Amrollahimiyandeh, Giti Paimard","doi":"10.1016/j.talanta.2024.126591","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126591","url":null,"abstract":"<p><p>Avian influenza viruses (AIV) are capable of infecting a considerable proportion of the world's population each year, leading to severe epidemics with high rates of morbidity and mortality. The methods now used to diagnose influenza virus A include the Western blot test (WB), hemagglutination inhibition (HI), and enzyme-linked immunosorbent assays (ELISAs). But because of their labor-intensiveness, lengthy procedures, need for costly equipment, and inexperienced staff, these approaches are considered inappropriate. The present review elucidates the recent advancements in the field of avian influenza detection through the utilization of nanomaterials-based immunosensors between 2014 and 2024. The classification of detection techniques has been taken into account to provide a comprehensive overview of the literature. The review encompasses a detailed illustration of the commonly employed detection mechanisms in immunosensors, namely, colorimetry, fluorescence assay, surface plasmon resonance (SPR), surface-enhanced Raman spectroscopy (SERS), electrochemical detection, quartz crystal microbalance (QCM) piezoelectric, and field-effect transistor (FET). Furthermore, the challenges and future prospects for the immunosensors have been deliberated upon. The present review aims to enhance the understanding of immunosensors-based sensing platforms for virus detection and to stimulate the development of novel immunosensors by providing novel ideas and inspirations. Therefore, the aim of this paper is to provide an updated information about biosensors, as a recent detection technique of influenza with its details regarding the various types of biosensors, which can be used for this review.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141764760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-20DOI: 10.1016/j.talanta.2024.126590
Ana Ballester-Caudet, Samuel García-García, Miguel Del Nogal Sánchez, Encarnación Rodríguez-Gonzalo, José Luis Pérez Pavón
A fast and non-separative screening strategy is presented for the analysis of five urinary metabolites of polycyclic aromatic hydrocarbons (PAHs), namely 2-naphthol, 1-acenaphthenol, 2-hydroxyfluorene, 9-phenanthrol and 1-hydroxypyrene. These hydroxylated derivatives (OH-PAHs) were subjected to enzymatic hydrolysis and were extracted from urine using a liquid-liquid extraction (LLE). The profile signals were obtained by direct injection of the sample into a programmed temperature vaporizer coupled to a quadrupole mass spectrometer via a deactivated fused silica tubing (PTV-qMS). Semi-quantitative determination was carried out by means of partial least squares regression (PLS1) using urine samples free of the analytes and spiked at several uncorrelated concentration levels. The multivariate calibration models worked satisfactorily, with errors ranging between 30 and 33 % for all the analytes except for 1-acenaphthenol that provided an error of 39 % when external validation set was considered. The repeatability and reproducibility, expressed as relative standard deviation (RSD), were ranged between 8-16 % and 11-18 %, respectively. The proposed method could be a useful tool for semi-quantification purposes of five OH-PAHs in urine samples, identifying positive samples for subsequent further chromatographic separation (confirmation), thus saving time and costs.
{"title":"Development of a non-separative screening strategy based on mass spectrometry for the semi-quantification of urinary polycyclic aromatic hydrocarbon metabolites.","authors":"Ana Ballester-Caudet, Samuel García-García, Miguel Del Nogal Sánchez, Encarnación Rodríguez-Gonzalo, José Luis Pérez Pavón","doi":"10.1016/j.talanta.2024.126590","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126590","url":null,"abstract":"<p><p>A fast and non-separative screening strategy is presented for the analysis of five urinary metabolites of polycyclic aromatic hydrocarbons (PAHs), namely 2-naphthol, 1-acenaphthenol, 2-hydroxyfluorene, 9-phenanthrol and 1-hydroxypyrene. These hydroxylated derivatives (OH-PAHs) were subjected to enzymatic hydrolysis and were extracted from urine using a liquid-liquid extraction (LLE). The profile signals were obtained by direct injection of the sample into a programmed temperature vaporizer coupled to a quadrupole mass spectrometer via a deactivated fused silica tubing (PTV-qMS). Semi-quantitative determination was carried out by means of partial least squares regression (PLS1) using urine samples free of the analytes and spiked at several uncorrelated concentration levels. The multivariate calibration models worked satisfactorily, with errors ranging between 30 and 33 % for all the analytes except for 1-acenaphthenol that provided an error of 39 % when external validation set was considered. The repeatability and reproducibility, expressed as relative standard deviation (RSD), were ranged between 8-16 % and 11-18 %, respectively. The proposed method could be a useful tool for semi-quantification purposes of five OH-PAHs in urine samples, identifying positive samples for subsequent further chromatographic separation (confirmation), thus saving time and costs.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}