Stimulant laxatives (especially bisacodyl and sodium picosulfate) are frequently found to be adulterated into slimming foods, causing health-threatening effects to consumers. Sensitive, accurate, easy-to-operate and portable multiplex analytical techniques are still desired for the rapid screening of stimulant laxatives in slimming foods. In this work, a highly sensitive dual-modal colorimetric/photothermal lateral flow immunochromatographic assay (LFIA) was established based on facilely prepared concentrated gold nanoparticles (cAuNPs). Due to the enhanced local surface plasmon resonance and absorption intensity of our unique cAuNPs, this LFIA can simultaneously detect bisacodyl, bis-(p-hydroxyphenyl)-pyridyl-2-methane and sodium picosulfate in slimming food as low as 0.014-0.308 ng/mL by a smartphone-based photothermal device or 1.4-10 ng/mL by naked eyes. Compared with traditional AuNPs-LFIA, it demonstrated 8.48-10.27 times higher sensitivity in photothermal mode. Moreover, in the recovery test and blind sample analysis, this dual-modal LFIA exhibited reasonable recoveries (85.04 %-119.18 %) and high correlation with authorized LC-MS/MS method, suggesting high accuracy and applicability. This work offers a highly sensitive, accurate and convenient way for the on-site determination of stimulant laxatives, which can also be employed to detect other food hazards, environmental pollutants, or clinical analytes within a few minutes.
{"title":"A smartphone-based dual-modal lateral flow immunochromatographic assay for multiplex detection of illegal stimulant laxatives in slimming foods.","authors":"Shuiyuan Liang, Sha Chen, Zhenlin Xu, Jiahong Chen, Hongtao Lei, Tian Guan","doi":"10.1016/j.talanta.2024.127433","DOIUrl":"10.1016/j.talanta.2024.127433","url":null,"abstract":"<p><p>Stimulant laxatives (especially bisacodyl and sodium picosulfate) are frequently found to be adulterated into slimming foods, causing health-threatening effects to consumers. Sensitive, accurate, easy-to-operate and portable multiplex analytical techniques are still desired for the rapid screening of stimulant laxatives in slimming foods. In this work, a highly sensitive dual-modal colorimetric/photothermal lateral flow immunochromatographic assay (LFIA) was established based on facilely prepared concentrated gold nanoparticles (cAuNPs). Due to the enhanced local surface plasmon resonance and absorption intensity of our unique cAuNPs, this LFIA can simultaneously detect bisacodyl, bis-(p-hydroxyphenyl)-pyridyl-2-methane and sodium picosulfate in slimming food as low as 0.014-0.308 ng/mL by a smartphone-based photothermal device or 1.4-10 ng/mL by naked eyes. Compared with traditional AuNPs-LFIA, it demonstrated 8.48-10.27 times higher sensitivity in photothermal mode. Moreover, in the recovery test and blind sample analysis, this dual-modal LFIA exhibited reasonable recoveries (85.04 %-119.18 %) and high correlation with authorized LC-MS/MS method, suggesting high accuracy and applicability. This work offers a highly sensitive, accurate and convenient way for the on-site determination of stimulant laxatives, which can also be employed to detect other food hazards, environmental pollutants, or clinical analytes within a few minutes.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"285 ","pages":"127433"},"PeriodicalIF":5.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Filling the microchannel with negatively charged hydrogel can exhibit microsacle ion current rectification (ICR) behavior, which is attributed to the space negative charge and structural asymmetry of hydrogel. In this study, this character had been applied to develop a trypsin sensor for the first time. A hydrogel synthesized with bovine serum albumin (BSA) and glyoxal (BSAG hydrogel) was filled at the tip of microchannel firstly. Subsequently, the BSAG hydrogel-filled microchannel was immersed in a trypsin solution to hydrolyze the BSA within the BSAG hydrogel. This process changes the space charge density and pore size of the BSAG hydrogel-filled microchannel, leading to a change in microscale ICR, which can be used for quantifying trypsin. Then the key parameters affecting the sensing performance such as the concentration of BSA, strength of the electrolyte, pH and reaction time were optimized. The detection range was from 10.0 ng/mL to 100 μg/mL with a detection limit as low as 2.55 ng/mL (S/N = 3). Due to the distinctive three-dimensional pore structure of the hydrogel and the specificity of trypsin for BSA hydrolysis, the sensor exhibits high sensitivity and specificity, as well as remarkable reproducibility and stability. This sensor has been effectively used to measure trypsin levels in human serum samples.
{"title":"Sensitive trypsin sensor based on the regulation of microscale ionic current rectification by the selectivity hydrolysis of hydrogel filled in microchannel.","authors":"Huabin Cai, Runhao Yuan, Shaokun Huang, Yanling Huang, Cuiying Lin, Yue Lin, Fang Luo, Zhenyu Lin, Lixin Wang","doi":"10.1016/j.talanta.2024.127422","DOIUrl":"10.1016/j.talanta.2024.127422","url":null,"abstract":"<p><p>Filling the microchannel with negatively charged hydrogel can exhibit microsacle ion current rectification (ICR) behavior, which is attributed to the space negative charge and structural asymmetry of hydrogel. In this study, this character had been applied to develop a trypsin sensor for the first time. A hydrogel synthesized with bovine serum albumin (BSA) and glyoxal (BSAG hydrogel) was filled at the tip of microchannel firstly. Subsequently, the BSAG hydrogel-filled microchannel was immersed in a trypsin solution to hydrolyze the BSA within the BSAG hydrogel. This process changes the space charge density and pore size of the BSAG hydrogel-filled microchannel, leading to a change in microscale ICR, which can be used for quantifying trypsin. Then the key parameters affecting the sensing performance such as the concentration of BSA, strength of the electrolyte, pH and reaction time were optimized. The detection range was from 10.0 ng/mL to 100 μg/mL with a detection limit as low as 2.55 ng/mL (S/N = 3). Due to the distinctive three-dimensional pore structure of the hydrogel and the specificity of trypsin for BSA hydrolysis, the sensor exhibits high sensitivity and specificity, as well as remarkable reproducibility and stability. This sensor has been effectively used to measure trypsin levels in human serum samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"285 ","pages":"127422"},"PeriodicalIF":5.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Owing to the facile fabrication and surface modification, the cost-effective polymer nanopores are widely employed in unimolecular determination of biomacromolecules and selective sensing of small molecules, nanoparticles and biomarkers. However, the documented polymer nanochannels are generally microscale in length with low spatial resolution. We herein synthesized azobenzene side-chain polymer (Azo-PMA) and spin-coated on silicon nitride membrane to obtain a polymer film of nanoscale thickness for further nanopore generation via controlled dielectric breakdown (CDB) approach. The Azo-PMA nanopores demonstrate good ions transporting activities, pH tolerance and stability in high concentration of electrolyte with low ionic current noise. In addition, the azobenzene-containing polymer nanopores exhibit photo-response upon UV/Vis. light irradiation. The Azo-PMA nanopore devices are utilized for linear and quadruple nucleic acids discrimination, sensing of proteins with distinct shapes and sizes, as well as the single amino acid resolution with good capture rate and sensitivity. We established an unimolecular sensing platform using polymer nanopores for nucleic acids and proteins detection with good spatial resolution, which will be an addition for the nanopore-carrier material exploration and applications in potential genomics and proteomics with high spatiotemporal resolution and low cost.
{"title":"Azo-PMA nanopores of sub-20 nm length for unimolecular resolution of nucleic acids and proteins.","authors":"Guodong Qi, Xun Chen, Daixin Liu, Meili Ren, Rong Tian, Liyuan Liang","doi":"10.1016/j.talanta.2024.127402","DOIUrl":"10.1016/j.talanta.2024.127402","url":null,"abstract":"<p><p>Owing to the facile fabrication and surface modification, the cost-effective polymer nanopores are widely employed in unimolecular determination of biomacromolecules and selective sensing of small molecules, nanoparticles and biomarkers. However, the documented polymer nanochannels are generally microscale in length with low spatial resolution. We herein synthesized azobenzene side-chain polymer (Azo-PMA) and spin-coated on silicon nitride membrane to obtain a polymer film of nanoscale thickness for further nanopore generation via controlled dielectric breakdown (CDB) approach. The Azo-PMA nanopores demonstrate good ions transporting activities, pH tolerance and stability in high concentration of electrolyte with low ionic current noise. In addition, the azobenzene-containing polymer nanopores exhibit photo-response upon UV/Vis. light irradiation. The Azo-PMA nanopore devices are utilized for linear and quadruple nucleic acids discrimination, sensing of proteins with distinct shapes and sizes, as well as the single amino acid resolution with good capture rate and sensitivity. We established an unimolecular sensing platform using polymer nanopores for nucleic acids and proteins detection with good spatial resolution, which will be an addition for the nanopore-carrier material exploration and applications in potential genomics and proteomics with high spatiotemporal resolution and low cost.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"285 ","pages":"127402"},"PeriodicalIF":5.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-21DOI: 10.1016/j.talanta.2025.127764
Yujie Liu , Chenran Zhang , Kun Zhang
Metal-organic framework (MOF)-based mimics are considered star materials to replace natural enzymes. However, their activity is generally limited to acidic conditions, which severely restricts their applications in biological systems where neutral pH is commonly required. Herein, a Zr(IV)-based MOF (MOF-808)/gold nanoparticle (AuNP) hybrid (called Hybrid-60) that shows superior peroxidase-like (POD-like) activity in both acidic and neutral media was prepared by in-situ growth of ultra-small AuNPs (UsAuNPs, ∼3.5 nm) on MOF-808. In comparison with the conventional AuNPs and MOF-808 nanozymes, Hybrid-60 demonstrated ∼8.04- and ∼6.74-time enhanced POD-like activities and superior high activity under neutral conditions, which broke the pH limitation. Furthermore, Hybrid-60 exhibited good tolerance to extreme pH value, concentrated salinity, and high-temperature environments. Taking Staphylococcus aureus (S. aureus) as a model analyte, we developed a simple immune sandwich assay using Hybrid-60 as colorimetric nanotags (ISAHC). A dual recognition strategy using anti-S. aureus antibody and concanavalin A-labeled Hybrid-60 was proposed to specifically capture and high-affinity label the target S. aureus. Then, leveraging the high POD-like activity of Hybrid-60, a simple and specific detection of S. aureus at nearly neutral pH was realized with a wide linear range (1 × 102–1 × 105 CFU/mL) and a low detection limit (32 CFU/mL). Moreover, the ISAHC method enabled one to detect the target S. aureus in human urine and serum with satisfactory recoveries from 93.8 % to 111.0 %, which indicates its clinical applicability. This study provides a new approach to develop neutral nanozymes and facilitate the point-of-care detection of bacteria.
{"title":"Ultra-small gold nanoparticle-coupled MOF-808 enabled sensitive detection of bacteria at neutral pH","authors":"Yujie Liu , Chenran Zhang , Kun Zhang","doi":"10.1016/j.talanta.2025.127764","DOIUrl":"10.1016/j.talanta.2025.127764","url":null,"abstract":"<div><div>Metal-organic framework (MOF)-based mimics are considered star materials to replace natural enzymes. However, their activity is generally limited to acidic conditions, which severely restricts their applications in biological systems where neutral pH is commonly required. Herein, a Zr(IV)-based MOF (MOF-808)/gold nanoparticle (AuNP) hybrid (called Hybrid-60) that shows superior peroxidase-like (POD-like) activity in both acidic and neutral media was prepared by <em>in-situ</em> growth of ultra-small AuNPs (UsAuNPs, ∼3.5 nm) on MOF-808. In comparison with the conventional AuNPs and MOF-808 nanozymes, Hybrid-60 demonstrated ∼8.04- and ∼6.74-time enhanced POD-like activities and superior high activity under neutral conditions, which broke the pH limitation. Furthermore, Hybrid-60 exhibited good tolerance to extreme pH value, concentrated salinity, and high-temperature environments. Taking <em>Staphylococcus aureus</em> (<em>S. aureus</em>) as a model analyte, we developed a simple <strong>i</strong>mmune <strong>s</strong>andwich <strong>a</strong>ssay using <strong>H</strong>ybrid-60 as <strong>c</strong>olorimetric nanotags (ISAHC). A dual recognition strategy using <em>anti</em>-<em>S. aureus</em> antibody and concanavalin A-labeled Hybrid-60 was proposed to specifically capture and high-affinity label the target <em>S. aureus.</em> Then, leveraging the high POD-like activity of Hybrid-60, a simple and specific detection of <em>S. aureus</em> at nearly neutral pH was realized with a wide linear range (1 × 10<sup>2</sup>–1 × 10<sup>5</sup> CFU/mL) and a low detection limit (32 CFU/mL). Moreover, the ISAHC method enabled one to detect the target <em>S. aureus</em> in human urine and serum with satisfactory recoveries from 93.8 % to 111.0 %, which indicates its clinical applicability. This study provides a new approach to develop neutral nanozymes and facilitate the point-of-care detection of bacteria.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"289 ","pages":"Article 127764"},"PeriodicalIF":5.6,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-18DOI: 10.1016/j.talanta.2025.127747
Víctor Ruiz-Valdepeñas Montiel , Eduardo Garcia-Calvo , Maria Gamella , Aina García-García , Santiago Rodríguez , Teresa García , José M. Pingarrón , Rosario Martín , Susana Campuzano
The only treatment to effectively manage celiac disease is the avoidance of gluten containing foods. Therefore, and given its high prevalence, it is of utmost importance to have reliable and efficient methods for the detection of gluten to ensure the well-being and quality of life of celiacs. This work presents the development of an electrochemical immunoplatform exhibiting many practical advantages including simplicity, reduced cost and high sensitivity for the screening of gluten-containing products. The methodology exploited the unique features offered by a recombinant antibody fragment with high affinity towards gliadin together with the use of magnetic microcarriers (MμCs) as scaffolds for the implementation of an indirect competitive immunoassay. Using amperometric transduction on disposable electrodes and the horseradish peroxidase/hydrogen peroxide/hydroquinone system, a dynamic range between 7.3 and 1982 ng mL−1 was obtained for gliadin standards, with a limit of detection of 1.4 ng mL−1. The developed immunoplatform was successfully employed for the analysis of a variety of processed foodstuffs, demonstrating the ability to discriminate between gluten-free and gluten-containing foods according to the legislated threshold (20 mg kg−1 of gluten). The agreement with the results provided by the R5-based ELISA and qPCR methods confirmed the suitability of the bioplatform as a competitive tool in terms of assay time (results in just 60 min after gliadin extraction) sensitivity and applicability, even at the point of need.
{"title":"Electrochemical tracking of gluten in marketed foods by using a recombinant antibody fragment based-platform","authors":"Víctor Ruiz-Valdepeñas Montiel , Eduardo Garcia-Calvo , Maria Gamella , Aina García-García , Santiago Rodríguez , Teresa García , José M. Pingarrón , Rosario Martín , Susana Campuzano","doi":"10.1016/j.talanta.2025.127747","DOIUrl":"10.1016/j.talanta.2025.127747","url":null,"abstract":"<div><div>The only treatment to effectively manage celiac disease is the avoidance of gluten containing foods. Therefore, and given its high prevalence, it is of utmost importance to have reliable and efficient methods for the detection of gluten to ensure the well-being and quality of life of celiacs. This work presents the development of an electrochemical immunoplatform exhibiting many practical advantages including simplicity, reduced cost and high sensitivity for the screening of gluten-containing products. The methodology exploited the unique features offered by a recombinant antibody fragment with high affinity towards gliadin together with the use of magnetic microcarriers (MμCs) as scaffolds for the implementation of an indirect competitive immunoassay. Using amperometric transduction on disposable electrodes and the horseradish peroxidase/hydrogen peroxide/hydroquinone system, a dynamic range between 7.3 and 1982 ng mL<sup>−1</sup> was obtained for gliadin standards, with a limit of detection of 1.4 ng mL<sup>−1</sup>. The developed immunoplatform was successfully employed for the analysis of a variety of processed foodstuffs, demonstrating the ability to discriminate between gluten-free and gluten-containing foods according to the legislated threshold (20 mg kg<sup>−1</sup> of gluten). The agreement with the results provided by the R5-based ELISA and qPCR methods confirmed the suitability of the bioplatform as a competitive tool in terms of assay time (results in just 60 min after gliadin extraction) sensitivity and applicability, even at the point of need.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127747"},"PeriodicalIF":5.6,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-17DOI: 10.1016/j.talanta.2025.127739
Mareeswaran Jeyaraman, Evgeni Eltzov
This study introduces a biosensor system designed for the rapid and specific detection of Bacillus subtilis (B. subtilis) in various food matrices, addressing the critical need for enhanced food safety measures. Recognizing the global prevalence of foodborne illnesses and the role of B. subtilis as a contributor, this research focused on developing a sensor capable of operating effectively in complex food environments such as rice and milk. The biosensor, utilizing an alginate-gelatin layer, demonstrated a high degree of specificity and sensitivity, distinguishing B. subtilis from other common foodborne pathogens like Bacillus licheniformis (B. licheniformis), Bacillus cereus (B. cereus), and Escherichia coli (E. coli). Through rigorous testing, the biosensor showed a distinct and rapid response to B. subtilis, even at lower bacterial concentrations, highlighting its potential for early detection of contamination. The study also explored the sensor's response across different food types, revealing the influence of food composition on pathogen detection efficacy. The results confirmed the biosensor's capability to adapt to varying food matrices, maintaining accuracy and reliability. This research contributes to the field of food safety, offering a practical solution for timely pathogen detection. The development of this biosensor represents a step forward in ensuring food quality and public health, providing a tool for the food industry to identify and mitigate potential contamination risks rapidly. These findings provide a foundation for the development of advanced on-site testing technologies, potentially enhancing food safety protocols and practices.
{"title":"Development of the alginate-gelatin-based biosensor for quick B. subtilis detection in foods.","authors":"Mareeswaran Jeyaraman, Evgeni Eltzov","doi":"10.1016/j.talanta.2025.127739","DOIUrl":"https://doi.org/10.1016/j.talanta.2025.127739","url":null,"abstract":"<p><p>This study introduces a biosensor system designed for the rapid and specific detection of Bacillus subtilis (B. subtilis) in various food matrices, addressing the critical need for enhanced food safety measures. Recognizing the global prevalence of foodborne illnesses and the role of B. subtilis as a contributor, this research focused on developing a sensor capable of operating effectively in complex food environments such as rice and milk. The biosensor, utilizing an alginate-gelatin layer, demonstrated a high degree of specificity and sensitivity, distinguishing B. subtilis from other common foodborne pathogens like Bacillus licheniformis (B. licheniformis), Bacillus cereus (B. cereus), and Escherichia coli (E. coli). Through rigorous testing, the biosensor showed a distinct and rapid response to B. subtilis, even at lower bacterial concentrations, highlighting its potential for early detection of contamination. The study also explored the sensor's response across different food types, revealing the influence of food composition on pathogen detection efficacy. The results confirmed the biosensor's capability to adapt to varying food matrices, maintaining accuracy and reliability. This research contributes to the field of food safety, offering a practical solution for timely pathogen detection. The development of this biosensor represents a step forward in ensuring food quality and public health, providing a tool for the food industry to identify and mitigate potential contamination risks rapidly. These findings provide a foundation for the development of advanced on-site testing technologies, potentially enhancing food safety protocols and practices.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"127739"},"PeriodicalIF":5.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-17DOI: 10.1016/j.talanta.2025.127757
Wei Wang , Jian Fang , Yizhuo Fan , Qilin Wang , Yu Chen , Shengping Ruan
The development of specialized high-performance sensors is an urgent requirement in the chemical and food safety fields. Yb2O3 is a trivalent rare earth oxide with catalytic effect, which has exploration and research value as a dopant for gas detection. Here, we constructed a high-performance triethylamine sensor using Yb2O3-regulated ZIF-derived ZnO nanocages. Characterization results exhibit the increase of the oxygen vacancies and chemisorbed oxygen in the composite material. Density functional theory (DFT) calculation results show that the Yb2O3 (222) surface has stronger adsorption and activation effects on oxygen molecules and more charge transfer than ZnO. Combined with the energy band modulation and the catalytic effect of the intrinsic oxygen vacancies of Yb2O3 on N–H bonds, the sensor has significantly lower operating temperature of 120 °C (60 °C lower than ZnO), higher response of 494.21 (20.34 times higher than ZnO), while also having better selectivity and low detection limit (488 ppb) for triethylamine. Finally, we applied the sensor to the freshness assessment of crucian carp and the sensor based on Yb2O3–ZnO composite showed good time linear relationship.
{"title":"The regulation of ZIF-derived ZnO nanocages by Yb2O3 for high-performance triethylamine sensing and fish freshness assessment","authors":"Wei Wang , Jian Fang , Yizhuo Fan , Qilin Wang , Yu Chen , Shengping Ruan","doi":"10.1016/j.talanta.2025.127757","DOIUrl":"10.1016/j.talanta.2025.127757","url":null,"abstract":"<div><div>The development of specialized high-performance sensors is an urgent requirement in the chemical and food safety fields. Yb<sub>2</sub>O<sub>3</sub> is a trivalent rare earth oxide with catalytic effect, which has exploration and research value as a dopant for gas detection. Here, we constructed a high-performance triethylamine sensor using Yb<sub>2</sub>O<sub>3</sub>-regulated ZIF-derived ZnO nanocages. Characterization results exhibit the increase of the oxygen vacancies and chemisorbed oxygen in the composite material. Density functional theory (DFT) calculation results show that the Yb<sub>2</sub>O<sub>3</sub> (222) surface has stronger adsorption and activation effects on oxygen molecules and more charge transfer than ZnO. Combined with the energy band modulation and the catalytic effect of the intrinsic oxygen vacancies of Yb<sub>2</sub>O<sub>3</sub> on N–H bonds, the sensor has significantly lower operating temperature of 120 °C (60 °C lower than ZnO), higher response of 494.21 (20.34 times higher than ZnO), while also having better selectivity and low detection limit (488 ppb) for triethylamine. Finally, we applied the sensor to the freshness assessment of crucian carp and the sensor based on Yb<sub>2</sub>O<sub>3</sub>–ZnO composite showed good time linear relationship.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127757"},"PeriodicalIF":5.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-17DOI: 10.1016/j.talanta.2025.127752
Wenxi Zhang , Jing Hu , Diancheng Zhu, Linyan Gu, Miao Cheng, Tao Wei, Qianqian Liu, Ruirui Wang, Wanfei Li, Yun Ling, Bo Liu
The traditional drip coating approach for sensor fabrication encounters several drawbacks, notably uneven film deposition, material wastage and vulnerability of sensitive materials to detachment, which hinder the attainment of high-quality sensor devices. Herein, we reported a polypyrrole-cerous stannate (PPy-Ce2Sn2O7) sensor by in-situ electrochemical deposition of the composite on interdigital electrode. The obtained sensor device can be directly utilized for gas-humidity detection at room temperature (RT, 25 °C). A systematic characterization including SEM, TEM, XRD, FTIR and XPS have been conducted to confirm the composites' morphology and composition. The effects of deposition condition and proportion ratio within the PPy-Ce2Sn2O7 on sensing performance were investigated. The optimal PPy-Ce2Sn2O7 exhibited considerable sensitivity of 3.85 and 1.25–80 ppm NH3 and 40 ppm NO2 at RT, respectively. Additionally, the sensor showed a limit of detection (LOD) of 74.8 ppb and 3.2-fold as well as 2.5-fold increase in response to 100 ppm NH3 when compared to pure PPy and Ce2Sn2O7. The influence of humidity on the electrical signal of the PPy-Ce2Sn2O7 is opposite to that of ammonia but similar to that of NO2. Intriguingly, the sensor exhibited an ultra-high response of 4392857 to 80 % relative humidity (RH), and it was successfully used for humidity warning and blowing monitoring. The sensor's excellent sensing performance is attributable to the large surface area of PPy, abundant oxygen vacancy sites of Ce2Sn2O7, and synergistic effect of the p-p heterostructure. This work provides a foundation for the development of easy to prepare, low-cost and highly sensitive gas-humidity sensors.
{"title":"In-situ electrochemical deposition of PPy-Ce2Sn2O7 for highly sensitive detection of NH3 and humidity at room temperature","authors":"Wenxi Zhang , Jing Hu , Diancheng Zhu, Linyan Gu, Miao Cheng, Tao Wei, Qianqian Liu, Ruirui Wang, Wanfei Li, Yun Ling, Bo Liu","doi":"10.1016/j.talanta.2025.127752","DOIUrl":"10.1016/j.talanta.2025.127752","url":null,"abstract":"<div><div>The traditional drip coating approach for sensor fabrication encounters several drawbacks, notably uneven film deposition, material wastage and vulnerability of sensitive materials to detachment, which hinder the attainment of high-quality sensor devices. Herein, we reported a polypyrrole-cerous stannate (PPy-Ce<sub>2</sub>Sn<sub>2</sub>O<sub>7</sub>) sensor by in-situ electrochemical deposition of the composite on interdigital electrode. The obtained sensor device can be directly utilized for gas-humidity detection at room temperature (RT, 25 °C). A systematic characterization including SEM, TEM, XRD, FTIR and XPS have been conducted to confirm the composites' morphology and composition. The effects of deposition condition and proportion ratio within the PPy-Ce<sub>2</sub>Sn<sub>2</sub>O<sub>7</sub> on sensing performance were investigated. The optimal PPy-Ce<sub>2</sub>Sn<sub>2</sub>O<sub>7</sub> exhibited considerable sensitivity of 3.85 and 1.25–80 ppm NH<sub>3</sub> and 40 ppm NO<sub>2</sub> at RT, respectively. Additionally, the sensor showed a limit of detection (LOD) of 74.8 ppb and 3.2-fold as well as 2.5-fold increase in response to 100 ppm NH<sub>3</sub> when compared to pure PPy and Ce<sub>2</sub>Sn<sub>2</sub>O<sub>7</sub>. The influence of humidity on the electrical signal of the PPy-Ce<sub>2</sub>Sn<sub>2</sub>O<sub>7</sub> is opposite to that of ammonia but similar to that of NO<sub>2</sub>. Intriguingly, the sensor exhibited an ultra-high response of 4392857 to 80 % relative humidity (RH), and it was successfully used for humidity warning and blowing monitoring. The sensor's excellent sensing performance is attributable to the large surface area of PPy, abundant oxygen vacancy sites of Ce<sub>2</sub>Sn<sub>2</sub>O<sub>7</sub>, and synergistic effect of the p-p heterostructure. This work provides a foundation for the development of easy to prepare, low-cost and highly sensitive gas-humidity sensors.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127752"},"PeriodicalIF":5.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-16DOI: 10.1016/j.talanta.2025.127737
Yimiao He , Feng Gong , Kebing Yi , Yu Feng , Ziwen Tang , Fuxiang Zhou , Xinghu Ji , Zhike He
Protein-conjugated gold nanoparticles (protein-Au NPs) have been extensively applied in the field of biochemistry due to their unique properties. It is of great significance to regulate the protein loading, reduce the loss of protein activity, and enhance the stability and accessibility of protein-Au NPs for their biochemical application. Herein, we investigated the freezing-assisted strategy for binding proteins to Au NPs, which was effective for various proteins and Au NPs with different sizes. The protein-Au NPs prepared by this freezing strategy exhibited better stability and higher protein loading compared to those prepared by typical direct adsorption (shaking) strategy. Based on this, we proposed a freezing-assisted and affinity-mediated strategy to conjugate proteins to Au NPs. In this strategy, biotinylated BSA (BSA-Bio) was employed as a mediator to bind protein to Au NPs through bioaffinity interaction. By attaching streptavidin-conjugated HRP (SA-HRP) onto Au NPs in this way, a nanoparticle denoted as Au NPs@BSA-Bio@SA-HRP was obtained. And we discovered that the protein loading of this nanoparticle prepared with 68 nm Au NPs was astonishingly 253 times higher than that of shaking strategy under the same conditions. In view of the advantages of this freezing-assisted and affinity-mediated strategy, we prepared antibody- and BSA-Bio-conjugated Au NPs for the immunoassay of interleukin-6 (IL-6). A limit of detection of 3.39 pg/mL was achieved, which was 7.4 times more sensitive than the conventional method. This study offered a new insight for protein conjugation and demonstrated a great potential for practical applications.
{"title":"Freezing-assisted and affinity-mediated conjugation strategy: Boosting protein loading on gold nanoparticles for enhanced immunoassay performance","authors":"Yimiao He , Feng Gong , Kebing Yi , Yu Feng , Ziwen Tang , Fuxiang Zhou , Xinghu Ji , Zhike He","doi":"10.1016/j.talanta.2025.127737","DOIUrl":"10.1016/j.talanta.2025.127737","url":null,"abstract":"<div><div>Protein-conjugated gold nanoparticles (protein-Au NPs) have been extensively applied in the field of biochemistry due to their unique properties. It is of great significance to regulate the protein loading, reduce the loss of protein activity, and enhance the stability and accessibility of protein-Au NPs for their biochemical application. Herein, we investigated the freezing-assisted strategy for binding proteins to Au NPs, which was effective for various proteins and Au NPs with different sizes. The protein-Au NPs prepared by this freezing strategy exhibited better stability and higher protein loading compared to those prepared by typical direct adsorption (shaking) strategy. Based on this, we proposed a freezing-assisted and affinity-mediated strategy to conjugate proteins to Au NPs. In this strategy, biotinylated BSA (BSA-Bio) was employed as a mediator to bind protein to Au NPs through bioaffinity interaction. By attaching streptavidin-conjugated HRP (SA-HRP) onto Au NPs in this way, a nanoparticle denoted as Au NPs@BSA-Bio@SA-HRP was obtained. And we discovered that the protein loading of this nanoparticle prepared with 68 nm Au NPs was astonishingly 253 times higher than that of shaking strategy under the same conditions. In view of the advantages of this freezing-assisted and affinity-mediated strategy, we prepared antibody- and BSA-Bio-conjugated Au NPs for the immunoassay of interleukin-6 (IL-6). A limit of detection of 3.39 pg/mL was achieved, which was 7.4 times more sensitive than the conventional method. This study offered a new insight for protein conjugation and demonstrated a great potential for practical applications.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127737"},"PeriodicalIF":5.6,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-16DOI: 10.1016/j.talanta.2025.127746
Chutimon Akkapinyo , Peter A. Lieberzeit , Peter Wolschann , Rungtiva P. Poo-arporn
Cancer antigen 15-3 (CA 15-3) is a critical biomarker for breast cancer, used to monitor disease severity and recurrence. Furthermore, its detection can be beneficial in post-operative treatment. Thus, biosensors that can track CA 15-3 levels in patients would provide useful data for disease monitoring. This study proposed molecularly imprinted polymer nanoparticles (nanoMIPs) specific for CA 15-3 detection; furthermore, the synthesized nanoMIPs were combined with an electrochemical sensor for breast cancer monitoring. The CA 15-3-specific nanoMIPs were generated via solid-phase synthesis. For sensor fabrication, a screen-printed carbon electrode (SPCE) was decorated with multi-walled carbon nanotubes and Au nanoparticles to improve the sensitivity. 4-aminothiophenol (4-ATP) enabled linking the synthesized CA 15-3-specific nanoMIPs to the electrode via the reaction with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). Characterizations via scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and the electrochemical method suggested the successful modification of the SPCE surface. Square wave voltammetry (SWV) was used to evaluate the sensor's performance in detecting CA 15-3. The sensor exhibited a wide detection range from 1 to 100 U/mL of CA 15-3 and a limit of detection (LOD) of 0.14 U/mL. The detection range covered the reference level (30 U/mL) of CA 15-3, allowing for distinguishing between healthy people and patients. The sensor allowed for the accurate and reliable determination of CA 15-3 concentrations in serum samples after pretreatment. In addition, the proposed sensor offers advantages in terms of easy fabrication and detection, low costs, and disposability. Therefore, it could serve as an alternative device for breast cancer monitoring.
{"title":"CA 15-3-specific molecularly imprinted polymer nanoparticles-based voltammetric sensor for breast cancer monitoring","authors":"Chutimon Akkapinyo , Peter A. Lieberzeit , Peter Wolschann , Rungtiva P. Poo-arporn","doi":"10.1016/j.talanta.2025.127746","DOIUrl":"10.1016/j.talanta.2025.127746","url":null,"abstract":"<div><div>Cancer antigen 15-3 (CA 15-3) is a critical biomarker for breast cancer, used to monitor disease severity and recurrence. Furthermore, its detection can be beneficial in post-operative treatment. Thus, biosensors that can track CA 15-3 levels in patients would provide useful data for disease monitoring. This study proposed molecularly imprinted polymer nanoparticles (nanoMIPs) specific for CA 15-3 detection; furthermore, the synthesized nanoMIPs were combined with an electrochemical sensor for breast cancer monitoring. The CA 15-3-specific nanoMIPs were generated via solid-phase synthesis. For sensor fabrication, a screen-printed carbon electrode (SPCE) was decorated with multi-walled carbon nanotubes and Au nanoparticles to improve the sensitivity. 4-aminothiophenol (4-ATP) enabled linking the synthesized CA 15-3-specific nanoMIPs to the electrode via the reaction with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). Characterizations via scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and the electrochemical method suggested the successful modification of the SPCE surface. Square wave voltammetry (SWV) was used to evaluate the sensor's performance in detecting CA 15-3. The sensor exhibited a wide detection range from 1 to 100 U/mL of CA 15-3 and a limit of detection (LOD) of 0.14 U/mL. The detection range covered the reference level (30 U/mL) of CA 15-3, allowing for distinguishing between healthy people and patients. The sensor allowed for the accurate and reliable determination of CA 15-3 concentrations in serum samples after pretreatment. In addition, the proposed sensor offers advantages in terms of easy fabrication and detection, low costs, and disposability. Therefore, it could serve as an alternative device for breast cancer monitoring.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127746"},"PeriodicalIF":5.6,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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