Pub Date : 2025-02-13DOI: 10.1016/j.talanta.2025.127714
Xiaofang Liu , Qun Wang , Huan Liu , Yong Zhang , Mei Yang , Danqun Huo , Changjun Hou
Timely detection of breast cancer not only positively influences patient treatment and life quality, but also possesses far-reaching implications for public health and medical research. And miRNAs, as emerging breast cancer-related biomarkers, demonstrate heightened sensitivity to disease progression. In this study, the same precursor was utilized to prepare micromaterials with large and small cavities, this was the safeguard for sensing probes and signals. And the signal amplification process was accomplished with strand displacement assistance by target excitation. Encouragingly, it was successfully and accurately detected miRNA-21 and miRNA-155 within 10 fmol/L - 10 nmol/L. Due to the unique sensing design, the signal can be read out not only by small fluorescent devices, but also by portable glucometers. The biosensor features several excellent working performances and illuminates resource-poor areas by equipping the most common glucometers. So, it is a sensitive, portable and promising biosensor.
{"title":"Signal-amplifying biosensors for miRNA detection and their extension in portable glucometer","authors":"Xiaofang Liu , Qun Wang , Huan Liu , Yong Zhang , Mei Yang , Danqun Huo , Changjun Hou","doi":"10.1016/j.talanta.2025.127714","DOIUrl":"10.1016/j.talanta.2025.127714","url":null,"abstract":"<div><div>Timely detection of breast cancer not only positively influences patient treatment and life quality, but also possesses far-reaching implications for public health and medical research. And miRNAs, as emerging breast cancer-related biomarkers, demonstrate heightened sensitivity to disease progression. In this study, the same precursor was utilized to prepare micromaterials with large and small cavities, this was the safeguard for sensing probes and signals. And the signal amplification process was accomplished with strand displacement assistance by target excitation. Encouragingly, it was successfully and accurately detected miRNA-21 and miRNA-155 within 10 fmol/L - 10 nmol/L. Due to the unique sensing design, the signal can be read out not only by small fluorescent devices, but also by portable glucometers. The biosensor features several excellent working performances and illuminates resource-poor areas by equipping the most common glucometers. So, it is a sensitive, portable and promising biosensor.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127714"},"PeriodicalIF":5.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-13DOI: 10.1016/j.talanta.2025.127744
Xiujuan Wang , Binghui Shi , Cengceng Xia , Mengnan Hou , Jingying Wang , Anning Tian , Chao Shi , Cuiping Ma
Traditional detection of foodborne pathogen relies on advanced analyzers, which is inadequate for the rapid control of infections, particularly in resource-limited regions, highlighting the necessity of developing detection systems for point-of-care testing (POCT). Herein, taking Vibrio parahaemolyticus as a detecting target, we reported poly-L-lysine functionalized silica membrane (PL-SM) based loop-mediated isothermal amplification (pLAMP) platform for sensitive on-site detection. This platform utilized PL-SM for DNA capture driven by the electrostatic attraction between protonated amine groups of poly-L-lysine and negatively charged phosphate groups of DNA, followed by introducing a colorimetric indicator calcein for LAMP amplification. After optimization, the colorimetric mode of pLAMP allowed the screening of V. parahaemolyticus with the visual limit of detection (vLOD) of 1 CFU/mL in 50 min, 1000-fold lower than methods based on commercial kits. Validation was performed using 174 seafoods, which was 97 % concordant to those of real-time PCR. Furthermore, an image processing approach was developed based on the analysis of the RGB under UV light. Paired with a smartphone, the objective analytical method could be readily conducted in the field. Thus, we propose a sensitive and visual detection platform, which may play a crucial role in improving testing efficiency and accuracy in food safety, medical diagnostics, and environmental monitoring.
{"title":"Poly-L-lysine functionalized silica membrane-enhanced colorimetric loop-mediated isothermal amplification for sensitive and rapid detection of Vibrio parahaemolyticus","authors":"Xiujuan Wang , Binghui Shi , Cengceng Xia , Mengnan Hou , Jingying Wang , Anning Tian , Chao Shi , Cuiping Ma","doi":"10.1016/j.talanta.2025.127744","DOIUrl":"10.1016/j.talanta.2025.127744","url":null,"abstract":"<div><div>Traditional detection of foodborne pathogen relies on advanced analyzers, which is inadequate for the rapid control of infections, particularly in resource-limited regions, highlighting the necessity of developing detection systems for point-of-care testing (POCT). Herein, taking <em>Vibrio parahaemolyticus</em> as a detecting target, we reported poly-<span>L</span>-lysine functionalized silica membrane (PL-SM) based loop-mediated isothermal amplification (pLAMP) platform for sensitive on-site detection. This platform utilized PL-SM for DNA capture driven by the electrostatic attraction between protonated amine groups of poly-L-lysine and negatively charged phosphate groups of DNA, followed by introducing a colorimetric indicator calcein for LAMP amplification. After optimization, the colorimetric mode of pLAMP allowed the screening of <em>V</em>. <em>parahaemolyticus</em> with the visual limit of detection (<em>v</em>LOD) of 1 CFU/mL in 50 min, 1000-fold lower than methods based on commercial kits. Validation was performed using 174 seafoods, which was 97 % concordant to those of real-time PCR. Furthermore, an image processing approach was developed based on the analysis of the RGB under UV light. Paired with a smartphone, the objective analytical method could be readily conducted in the field. Thus, we propose a sensitive and visual detection platform, which may play a crucial role in improving testing efficiency and accuracy in food safety, medical diagnostics, and environmental monitoring.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127744"},"PeriodicalIF":5.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-13DOI: 10.1016/j.talanta.2025.127738
Tomasz Durak , Beata Ciak , Roma Durak , Joanna Depciuch
ATR-Fourier transform infrared spectroscopy was used to determine the carbon and nitrogen content in the leaves of herbaceous forest plant species and functional traits associated with the leaf economic spectrum (one of the two-dimensional global spectrum of plant form and function), and monitoring plant physiological status under elevated temperature conditions. The content of carbon and nitrogen determined by traditional methods validated the accuracy of ATR-FTIR method. It was also shown that in the case of forest herbs, the ATR-FTIR method is an efficient tool for determining functional traits (such as specific leaf area (SLA) and leaf dry matter content (LDMC)) related to the leaf economics spectrum, and to diagnose the photophysiological state of plants after changes of temperature (changes of day/night temperature from 21/13 °C to 25/17 °C). Measuring the areas of three absorption bands of the ATR-FTIR spectra related to amides I and II (between 1700 cm−1 and 1500 cm−1), carbohydrates (cellulose and hemicellulose; between 1200 cm−1 - 850 cm−1) and amide III (between 1290 cm−1 and 1190 cm-1) allowed for determination of all analysed chemical and functional properties of leaves. Based on selected absorption bands accurately estimated C and N content, with coefficients of correlation (r) of 0.88 for C and 0.84 for N. SLA and LDMC were also predicted, with r values of 0.88 and −0.91, respectively. Moreover, ATR-FTIR proved to be a rapid, non-destructive tool for monitoring the plant physiological status, as demonstrated by the significant correlation (r = 0.99) between the chlorophyll fluorescence performance index (PI) and ATR-FTIR data. ATR-FTIR has been demonstrated as an efficient tool for simultaneous quantification of leaf carbon and nitrogen content, economic functional traits, and physiological status of forest herb plants.
{"title":"Application of ATR-Fourier transform infrared spectroscopy in fast and simultaneous determination of leaf chemical and functional properties of forest herb species","authors":"Tomasz Durak , Beata Ciak , Roma Durak , Joanna Depciuch","doi":"10.1016/j.talanta.2025.127738","DOIUrl":"10.1016/j.talanta.2025.127738","url":null,"abstract":"<div><div>ATR-Fourier transform infrared spectroscopy was used to determine the carbon and nitrogen content in the leaves of herbaceous forest plant species and functional traits associated with the leaf economic spectrum (one of the two-dimensional global spectrum of plant form and function), and monitoring plant physiological status under elevated temperature conditions. The content of carbon and nitrogen determined by traditional methods validated the accuracy of ATR-FTIR method. It was also shown that in the case of forest herbs, the ATR-FTIR method is an efficient tool for determining functional traits (such as specific leaf area (SLA) and leaf dry matter content (LDMC)) related to the leaf economics spectrum, and to diagnose the photophysiological state of plants after changes of temperature (changes of day/night temperature from 21/13 °C to 25/17 °C). Measuring the areas of three absorption bands of the ATR-FTIR spectra related to amides I and II (between 1700 cm<sup>−1</sup> and 1500 cm<sup>−1</sup>), carbohydrates (cellulose and hemicellulose; between 1200 cm<sup>−1</sup> - 850 cm<sup>−1</sup>) and amide III (between 1290 cm<sup>−1</sup> and 1190 cm-<sup>1</sup>) allowed for determination of all analysed chemical and functional properties of leaves. Based on selected absorption bands accurately estimated C and N content, with coefficients of correlation (r) of 0.88 for C and 0.84 for N. SLA and LDMC were also predicted, with r values of 0.88 and −0.91, respectively. Moreover, ATR-FTIR proved to be a rapid, non-destructive tool for monitoring the plant physiological status, as demonstrated by the significant correlation (r = 0.99) between the chlorophyll fluorescence performance index (PI) and ATR-FTIR data. ATR-FTIR has been demonstrated as an efficient tool for simultaneous quantification of leaf carbon and nitrogen content, economic functional traits, and physiological status of forest herb plants.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"289 ","pages":"Article 127738"},"PeriodicalIF":5.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-12DOI: 10.1016/j.talanta.2025.127711
Wafa Aidli, Silvia Comis, Luigi Falciola, Valentina Pifferi
A ratiometric electrochemical sensor featuring a benzoquinone-modified vertically aligned mesoporous silica film (BQ/VMSF) has been developed for the accurate detection of diclofenac (DCF), a widely utilized non-steroidal anti-inflammatory drug. The design process employed the Electrochemical assisted self-assembly (EASA) technique to grow a thiol-functionalized mesoporous silica film on a carbon screen-printed electrode, which was further modified with benzoquinone via click chemistry. Characterization using Transmission Electron Microscopy (TEM), Fourier-Transform Infrared Spectroscopy (FTIR), Cyclic Voltammetry (CV), and Electrochemical Impedance Spectroscopy (EIS) confirmed the successful synthesis and optimal functionality of the mesoporous structure. In this configuration, benzoquinone serves as an internal redox reference, generating one redox signal, while the electrochemical oxidation of diclofenac produces a second redox signal. The sensor's output is derived from the ratio of these two signals, IDCF/IBQ, enhancing its sensitivity and selectivity for diclofenac detection. The sensor, tested through Differential Pulse Voltammetry (DPV), exhibited a linear response range from 1 to 10 μM for diclofenac and a remarkably low limit of detection (LOD) of 0.73 μM. Application of the sensor for diclofenac analysis in pharmaceutical formulations demonstrated recovery rates ranging from 97.68 % to 101.79 % and relative errors below 2.32 %, affirming its practical utility.
{"title":"Benzoquinone-modified vertically aligned mesoporous silica for ratiometric electrochemical detection of diclofenac","authors":"Wafa Aidli, Silvia Comis, Luigi Falciola, Valentina Pifferi","doi":"10.1016/j.talanta.2025.127711","DOIUrl":"10.1016/j.talanta.2025.127711","url":null,"abstract":"<div><div>A ratiometric electrochemical sensor featuring a benzoquinone-modified vertically aligned mesoporous silica film (BQ/VMSF) has been developed for the accurate detection of diclofenac (DCF), a widely utilized non-steroidal anti-inflammatory drug. The design process employed the Electrochemical assisted self-assembly (EASA) technique to grow a thiol-functionalized mesoporous silica film on a carbon screen-printed electrode, which was further modified with benzoquinone via click chemistry. Characterization using Transmission Electron Microscopy (TEM), Fourier-Transform Infrared Spectroscopy (FTIR), Cyclic Voltammetry (CV), and Electrochemical Impedance Spectroscopy (EIS) confirmed the successful synthesis and optimal functionality of the mesoporous structure. In this configuration, benzoquinone serves as an internal redox reference, generating one redox signal, while the electrochemical oxidation of diclofenac produces a second redox signal. The sensor's output is derived from the ratio of these two signals, I<sub>DCF</sub>/I<sub>BQ</sub>, enhancing its sensitivity and selectivity for diclofenac detection. The sensor, tested through Differential Pulse Voltammetry (DPV), exhibited a linear response range from 1 to 10 μM for diclofenac and a remarkably low limit of detection (LOD) of 0.73 μM. Application of the sensor for diclofenac analysis in pharmaceutical formulations demonstrated recovery rates ranging from 97.68 % to 101.79 % and relative errors below 2.32 %, affirming its practical utility.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127711"},"PeriodicalIF":5.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-12DOI: 10.1016/j.talanta.2025.127733
Jia-Long Hao , Heng-Ci Tian , Chao Qi , Rui-Ying Li , Sen Hu , Yang-Ting Lin , Yong-Sheng He , Wei Yang
In-situ stable Mg isotope analysis of olivine, the most common mineral in igneous and metamorphic rocks, provides critical insights into their formation and timescales. However, accurate correction of significant matrix effects is hampered by the lack of olivine reference materials with forsterite (Fo) content below 80. More importantly, current techniques using secondary ion mass spectrometry (SIMS) or laser ablation-multi collector-ICP-MS (LA-MC-ICP-MS) are insufficient to decipher geological processes occurring at fine scales (<10 μm). Here, we report potential olivine reference materials with Fo contents ranging from nearly 0 to 90.8, covering a wide range and displaying homogeneous compositions within individual samples. These reference materials were developed through high-temperature experiments and the collection of extraterrestrial meteorites. Using these materials, we established a method for Mg isotope analysis on a CAMECA NanoSIMS 50L, for the first time achieving a high spatial resolution of ∼1 μm with a precision of 0.7–0.8 ‰ (1SD). We found that the matrix effect is best modelled by a BiHill equation with the 24Mg/(24Mg + 56Fe) ratio. Simultaneous detection of 24Mg–26Mg–28Si–56Fe by NanoSIMS enable us to calibrate the matrix effect online for olivine Mg-isotope analysis. This method was successfully applied to Chang'e−5 (CE5) lunar chemically-zoned olivine crystals, revealing substantial variation of δ26Mg (>4 ‰) on a micron scale (<100 μm).
{"title":"Micro-scale (1 μm) Mg isotope analysis of olivine by NanoSIMS with online matrix correction and its application to Chang'e−5 sample","authors":"Jia-Long Hao , Heng-Ci Tian , Chao Qi , Rui-Ying Li , Sen Hu , Yang-Ting Lin , Yong-Sheng He , Wei Yang","doi":"10.1016/j.talanta.2025.127733","DOIUrl":"10.1016/j.talanta.2025.127733","url":null,"abstract":"<div><div>In-situ stable Mg isotope analysis of olivine, the most common mineral in igneous and metamorphic rocks, provides critical insights into their formation and timescales. However, accurate correction of significant matrix effects is hampered by the lack of olivine reference materials with forsterite (Fo) content below 80. More importantly, current techniques using secondary ion mass spectrometry (SIMS) or laser ablation-multi collector-ICP-MS (LA-MC-ICP-MS) are insufficient to decipher geological processes occurring at fine scales (<10 μm). Here, we report potential olivine reference materials with Fo contents ranging from nearly 0 to 90.8, covering a wide range and displaying homogeneous compositions within individual samples. These reference materials were developed through high-temperature experiments and the collection of extraterrestrial meteorites. Using these materials, we established a method for Mg isotope analysis on a CAMECA NanoSIMS 50L, for the first time achieving a high spatial resolution of ∼1 μm with a precision of 0.7–0.8 ‰ (1SD). We found that the matrix effect is best modelled by a BiHill equation with the <sup>24</sup>Mg/(<sup>24</sup>Mg + <sup>56</sup>Fe) ratio. Simultaneous detection of <sup>24</sup>Mg–<sup>26</sup>Mg–<sup>28</sup>Si–<sup>56</sup>Fe by NanoSIMS enable us to calibrate the matrix effect online for olivine Mg-isotope analysis. This method was successfully applied to Chang'e−5 (CE5) lunar chemically-zoned olivine crystals, revealing substantial variation of δ<sup>26</sup>Mg (>4 ‰) on a micron scale (<100 μm).</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"289 ","pages":"Article 127733"},"PeriodicalIF":5.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-12DOI: 10.1016/j.talanta.2025.127703
Izabela I. Rzeznicka , Achmad Syarif Hidayat , Hideyuki Horino , Baatshwana Caroline Ditlhakanyane , Venecio U. Ultra Jr.
Africa has made significant progress in healthcare metrics over the past two decades. However, providing access and quality of healthcare to people living in rural regions remains a challenge. In this respect, so-called mobile health (mHealth) and smartphone-enabled diagnostics methods could be of great use in providing services, both necessary and novel, to rural populations. This review provides a basic background for understanding various concepts behind smartphone-enabled methods and summarizes existing examples in the fields of medical diagnostics and environmental monitoring. The aim is to engage young African students to contribute to the field but also to provide decision makers with ideas on low-cost solutions for rural healthcare.
{"title":"Smartphone-enabled medical diagnostics and environmental monitoring for rural Africa","authors":"Izabela I. Rzeznicka , Achmad Syarif Hidayat , Hideyuki Horino , Baatshwana Caroline Ditlhakanyane , Venecio U. Ultra Jr.","doi":"10.1016/j.talanta.2025.127703","DOIUrl":"10.1016/j.talanta.2025.127703","url":null,"abstract":"<div><div>Africa has made significant progress in healthcare metrics over the past two decades. However, providing access and quality of healthcare to people living in rural regions remains a challenge. In this respect, so-called mobile health (mHealth) and smartphone-enabled diagnostics methods could be of great use in providing services, both necessary and novel, to rural populations. This review provides a basic background for understanding various concepts behind smartphone-enabled methods and summarizes existing examples in the fields of medical diagnostics and environmental monitoring. The aim is to engage young African students to contribute to the field but also to provide decision makers with ideas on low-cost solutions for rural healthcare.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127703"},"PeriodicalIF":5.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-12DOI: 10.1016/j.talanta.2025.127735
Lianyu Bao , Fei Zhou , Ye Liu , Botian Wang , Rang Chu , Qingmao Zhang , Hongcheng Wang
Monitoring chemical reaction processes is of great significance in the study of the reaction mechanisms, and the optimization or control of reaction conditions. In this work, we demonstrate a novel monitoring method of chemical reactions using fiber SERS probes. The high-performance fiber SERS probes are prepared by the laser-induced evaporation self-assembly method (LIESAM), where lots of Au-nanorod clusters are deposited on the fiber facet for providing large SERS enhancement factor and good hot electron catalytic property. The remote and in-situ monitoring of chemical reactions is achieved through simply dipping the probes into the reaction solution. Taking the classic reduction reaction from 4-nitrothiophenol (4-NTP) to 4-aminothiophenol (4-ATP) by sodium borohydride (NaBH4) as an example, the time-resolved SERS spectra collected by probes clearly reflect the reduction procedure of the disappearance of 4-NTP, the formation of intermediate product 4,4′-dimercaptoazobenzene (4,4′-DMAB) and the emergence of the final product 4-ATP. In addition, a new Raman peak at 1366 cm−1 is observed when the 4-NTP is reduced in an H2 atmosphere generated by the hydrolysis of NaBH4, corresponding the formation of a new intermediate product 4-nitrosothiophenol (4-NSTP). Also, the monitoring of the oxidation reaction from 4-ATP to 4,4′-DMAB is demonstrated using fiber SERS probes. Considering that all the SERS spectra are acquired with a portable Raman spectrometer and the fiber has a very low transmission loss, this work provides a good platform for remote, on-site and in situ monitoring of chemical reactions in liquid environments and thus finds important applications in scientific research and industrial scenarios.
{"title":"Remote and in-situ monitoring of plasmon-induced catalysis reaction by fiber SERS probes","authors":"Lianyu Bao , Fei Zhou , Ye Liu , Botian Wang , Rang Chu , Qingmao Zhang , Hongcheng Wang","doi":"10.1016/j.talanta.2025.127735","DOIUrl":"10.1016/j.talanta.2025.127735","url":null,"abstract":"<div><div>Monitoring chemical reaction processes is of great significance in the study of the reaction mechanisms, and the optimization or control of reaction conditions. In this work, we demonstrate a novel monitoring method of chemical reactions using fiber SERS probes. The high-performance fiber SERS probes are prepared by the laser-induced evaporation self-assembly method (LIESAM), where lots of Au-nanorod clusters are deposited on the fiber facet for providing large SERS enhancement factor and good hot electron catalytic property. The remote and <em>in-situ</em> monitoring of chemical reactions is achieved through simply dipping the probes into the reaction solution. Taking the classic reduction reaction from 4-nitrothiophenol (4-NTP) to 4-aminothiophenol (4-ATP) by sodium borohydride (NaBH<sub>4</sub>) as an example, the time-resolved SERS spectra collected by probes clearly reflect the reduction procedure of the disappearance of 4-NTP, the formation of intermediate product 4,4′-dimercaptoazobenzene (4,4′-DMAB) and the emergence of the final product 4-ATP. In addition, a new Raman peak at 1366 cm<sup>−1</sup> is observed when the 4-NTP is reduced in an H<sub>2</sub> atmosphere generated by the hydrolysis of NaBH<sub>4</sub>, corresponding the formation of a new intermediate product 4-nitrosothiophenol (4-NSTP). Also, the monitoring of the oxidation reaction from 4-ATP to 4,4′-DMAB is demonstrated using fiber SERS probes. Considering that all the SERS spectra are acquired with a portable Raman spectrometer and the fiber has a very low transmission loss, this work provides a good platform for remote, on-site and <em>in situ</em> monitoring of chemical reactions in liquid environments and thus finds important applications in scientific research and industrial scenarios.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127735"},"PeriodicalIF":5.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Regular monitoring of serum antibody levels is crucial for preventing interference with therapeutic effectiveness and reducing the risk of toxicity. To address this, a CRISPR/Cas12a sensing system with circular CRISPR RNAs (CcrRNAs) is described for highly sensitive detection of anti-digoxin (Anti-Dig) antibodies in human serum. In this work, the topology structure of CcrRNAs effectively suppresses the function of linear crRNAs (LcrRNAs), making them unable to regulate the cis-/trans-cleavage activity of the Cas12a system. Therefore, a low-background is obtained in the absence of targets. The target Anti-Dig antibodies trigger the assembly of the complete multicomponent nucleic acid enzyme (MNAzyme) with active enzyme activity, which can transform CcrRNAs into LcrRNAs. The LcrRNAs further recover the trans-cleavage activity of the CRISPR/Cas12a system, which can degrade single-stranded reporter DNA to generate a significantly enhanced fluorescent signal. This method enables sensitive detection of Anti-Dig antibodies as low as 15 pM within 60 min and exhibits a linear detection range of 25 pM–50 nM. It also exhibits excellent selectivity against non-target antibodies and has been successfully validated in diluted serum samples, achieving a recovery rate ranging from 96.16 % to 103.08 %. This novel CRISPR/Cas12a sensing system with CcrRNA represents a powerful and efficient tool for detecting low-abundance biomarkers in complex biological samples.
{"title":"Low-background CRISPR/Cas12a sensing system with circular CRISPR RNA for amplified fluorescent detection of antibody in human serum","authors":"Qian Xiang , Fang Guo , Daxiu Li , Fengfeng Xu , Wenjiao Zhou","doi":"10.1016/j.talanta.2025.127730","DOIUrl":"10.1016/j.talanta.2025.127730","url":null,"abstract":"<div><div>Regular monitoring of serum antibody levels is crucial for preventing interference with therapeutic effectiveness and reducing the risk of toxicity. To address this, a CRISPR/Cas12a sensing system with circular CRISPR RNAs (<sup>C</sup>crRNAs) is described for highly sensitive detection of anti-digoxin (Anti-Dig) antibodies in human serum. In this work, the topology structure of <sup>C</sup>crRNAs effectively suppresses the function of linear crRNAs (<sup>L</sup>crRNAs), making them unable to regulate the <em>cis</em>-/<em>trans</em>-cleavage activity of the Cas12a system. Therefore, a low-background is obtained in the absence of targets. The target Anti-Dig antibodies trigger the assembly of the complete multicomponent nucleic acid enzyme (MNAzyme) with active enzyme activity, which can transform <sup>C</sup>crRNAs into <sup>L</sup>crRNAs. The <sup>L</sup>crRNAs further recover the <em>trans</em>-cleavage activity of the CRISPR/Cas12a system, which can degrade single-stranded reporter DNA to generate a significantly enhanced fluorescent signal. This method enables sensitive detection of Anti-Dig antibodies as low as 15 pM within 60 min and exhibits a linear detection range of 25 pM–50 nM. It also exhibits excellent selectivity against non-target antibodies and has been successfully validated in diluted serum samples, achieving a recovery rate ranging from 96.16 % to 103.08 %. This novel CRISPR/Cas12a sensing system with <sup>C</sup>crRNA represents a powerful and efficient tool for detecting low-abundance biomarkers in complex biological samples.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127730"},"PeriodicalIF":5.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-12DOI: 10.1016/j.talanta.2025.127707
Yuanhang Wang , Yuwei Tian , Cong Liu , Zhixing Gao , Zhenlin Hu , Hongyu He , Daqing Yuan , Zhao Wang
To ensure the safe operation of pressurized water reactors, the real-time and high-sensitive monitoring of Li and Zn in primary cooling water is required. Laser-induced breakdown spectroscopy (LIBS) is a real-time analytical method with great application potential for elemental monitoring in primary cooling water. However, when LIBS directly detects liquids, plasma quenching will reduce the detection sensitivity. In this work, capillary effect-enhanced LIBS (CE-LIBS) was proposed to improve the detection sensitivity of elements in aqueous solutions. Titanium foam substrates were used to enrich the solutes in the solutions. The signal enhancement mechanisms of the method were analyzed. The results showed that the solutes were enriched on the surface of the titanium foam substrate due to the capillary effect. The excitation temperature and electron density of the laser-induced plasma were both increased by the titanium foam substrate, thus increasing the plasma emission intensity. The limits of detection of Li and Zn were 0.41 and 3.83 ng/mL by using CE-LIBS, which can fully meet the requirements of Li and Zn monitoring in primary cooling water. The practicability of the method was demonstrated by analyzing simulated primary cooling water samples, and the recovery values of Li and Zn were in the range of 92–105 % and 96–102 %, respectively. The CE-LIBS does not require additional treatment of the substrate, and the detection process is simple and fast. Results indicated that the CE-LIBS method has broad application prospects in the real-time and high-sensitivity detection of elements in aqueous samples.
{"title":"High-sensitive detection of Li and Zn in aqueous solutions using capillary effect-enhanced laser-induced breakdown spectroscopy","authors":"Yuanhang Wang , Yuwei Tian , Cong Liu , Zhixing Gao , Zhenlin Hu , Hongyu He , Daqing Yuan , Zhao Wang","doi":"10.1016/j.talanta.2025.127707","DOIUrl":"10.1016/j.talanta.2025.127707","url":null,"abstract":"<div><div>To ensure the safe operation of pressurized water reactors, the real-time and high-sensitive monitoring of Li and Zn in primary cooling water is required. Laser-induced breakdown spectroscopy (LIBS) is a real-time analytical method with great application potential for elemental monitoring in primary cooling water. However, when LIBS directly detects liquids, plasma quenching will reduce the detection sensitivity. In this work, capillary effect-enhanced LIBS (CE-LIBS) was proposed to improve the detection sensitivity of elements in aqueous solutions. Titanium foam substrates were used to enrich the solutes in the solutions. The signal enhancement mechanisms of the method were analyzed. The results showed that the solutes were enriched on the surface of the titanium foam substrate due to the capillary effect. The excitation temperature and electron density of the laser-induced plasma were both increased by the titanium foam substrate, thus increasing the plasma emission intensity. The limits of detection of Li and Zn were 0.41 and 3.83 ng/mL by using CE-LIBS, which can fully meet the requirements of Li and Zn monitoring in primary cooling water. The practicability of the method was demonstrated by analyzing simulated primary cooling water samples, and the recovery values of Li and Zn were in the range of 92–105 % and 96–102 %, respectively. The CE-LIBS does not require additional treatment of the substrate, and the detection process is simple and fast. Results indicated that the CE-LIBS method has broad application prospects in the real-time and high-sensitivity detection of elements in aqueous samples.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127707"},"PeriodicalIF":5.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xylazine is a widely employed veterinary anesthetic that, in recent years, has also been used as a recreational drug, leading to several fatal and non-fatal intoxications. We present herein a comprehensive investigation of the electrochemical behavior of xylazine, followed by the quantification of this target analyte. Specifically, it has been found that xylazine oxidizes irreversibly on a glassy carbon electrode in ethanol/lithium perchlorate media, with an EC mechanism; the number of electrons involved in the process is equal to two. The oxidation pathway has been suggested and supported by a thorough electrochemical investigation, in which the behavior of the analyte has been compared with other model compounds.
A quantitative method was then proposed, employing differential pulse voltammetry (DPV) for the determination of xylazine in real samples. The analytical figures of merits were computed from the calibration curves obtained by analyzing standard solutions of xylazine in ethanol/lithium perchlorate medium. A LOQ of 0.2 μg mL−1 and a dynamic range up to 150 μg mL−1 were achieved. The method was then applied to samples simulating "street tablets” and urine from drug consumers. The selectivity of the method, as well as the reproducibility of the results, were investigated by analyzing the interferences between common excipients and human urine components. For urine samples, a Florisil-based clean-up and preconcentration step by solid phase extraction (SPE) were carried out. The SPE strategy was optimized by a Design of Experiments (DoE) approach, whereby an enrichment factor of up to 25 was obtained.
Recoveries from 87 % to 108 % were achieved on all examined samples, suggesting the good potential of the method for its application in clinical and forensic chemistry.
{"title":"New psychoactive drugs: A comprehensive electrochemical characterization and differential pulse voltammetric analysis of xylazine","authors":"Alessandra Bonanni , Giancarla Alberti , Protti Stefano , Teresa Franceschini , Daniele Merli","doi":"10.1016/j.talanta.2025.127710","DOIUrl":"10.1016/j.talanta.2025.127710","url":null,"abstract":"<div><div>Xylazine is a widely employed veterinary anesthetic that, in recent years, has also been used as a recreational drug, leading to several fatal and non-fatal intoxications. We present herein a comprehensive investigation of the electrochemical behavior of xylazine, followed by the quantification of this target analyte. Specifically, it has been found that xylazine oxidizes irreversibly on a glassy carbon electrode in ethanol/lithium perchlorate media, with an EC mechanism; the number of electrons involved in the process is equal to two. The oxidation pathway has been suggested and supported by a thorough electrochemical investigation, in which the behavior of the analyte has been compared with other model compounds.</div><div>A quantitative method was then proposed, employing differential pulse voltammetry (DPV) for the determination of xylazine in real samples. The analytical figures of merits were computed from the calibration curves obtained by analyzing standard solutions of xylazine in ethanol/lithium perchlorate medium. A LOQ of 0.2 μg mL<sup>−1</sup> and a dynamic range up to 150 μg mL<sup>−1</sup> were achieved. The method was then applied to samples simulating \"street tablets” and urine from drug consumers. The selectivity of the method, as well as the reproducibility of the results, were investigated by analyzing the interferences between common excipients and human urine components. For urine samples, a Florisil-based clean-up and preconcentration step by solid phase extraction (SPE) were carried out. The SPE strategy was optimized by a Design of Experiments (DoE) approach, whereby an enrichment factor of up to 25 was obtained.</div><div>Recoveries from 87 % to 108 % were achieved on all examined samples, suggesting the good potential of the method for its application in clinical and forensic chemistry.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127710"},"PeriodicalIF":5.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号