Talanta最新文献

{"title":"Systematic investigation of analyte protectants to overcome matrix effects for gas chromatographic analysis of tobacco alkaloids and primary aromatic amines across diverse matrices","authors":"Yaqiong Qin ,&nbsp;Xiaoyu Wang ,&nbsp;Lining Pan ,&nbsp;Rana Tayyarah ,&nbsp;Zhiguo Wang ,&nbsp;Yu Liu ,&nbsp;Ran Wan ,&nbsp;Haijian Su ,&nbsp;Xuejie Li ,&nbsp;Li Chen ,&nbsp;Huapeng Cui","doi":"10.1016/j.talanta.2025.129343","DOIUrl":"10.1016/j.talanta.2025.129343","url":null,"abstract":"<div><div>The application of analyte protectants (APs) is recognized as an effective and practical strategy to enhance analyte signals in matrix-free solutions and compensate for matrix effects (MEs) in gas chromatography (GC) analysis. However, systematic investigations into promising APs for basic analytes and elucidation of the underlying molecular mechanisms remain limited. In this study, two classes of basic analytes with diverse polarity and basicity, including tobacco alkaloids (TAs) and primary aromatic amines (PAAs), were selected as target analytes. A total of 29 candidates covering a broad range of polarity, basicity, and volatility were systematically evaluated to identify the promising APs for TAs and PAAs. According to individual and average analyte peak enhancement factors, several polyamines, including diethylenetriamine, tris(2-aminoethyl)amine, spermine, pentaethylenehexamine, and the diamine 1,11-diaminoundecane, were identified as the potential APs. Corresponding combinations of these APs for TAs and PAAs at optimized concentrations were validated to significantly reduce the limits of quantification from 1.58 to 307.85 to 1.26–43.43 ng/mL in solvent and improve recovery rates from 92.3 to 352.1 to 80.3–111.5 % in complex matrices. Consistent signal responses across diverse matrices further highlight the developed APs effectively overcome the MEs, ensuring the accurate and robust determinations in practical applications. Furthermore, density functional theory (DFT) calculations revealed that the superior enhancement effects of these APs are primarily attributed to lower interaction energies and stronger hydrogen-bonding interactions with silanol groups in the GC system.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129343"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of an indirect competitive ELISA for cyenopyrafen detection using a novel monoclonal antibody","authors":"Tianyu Lan ,&nbsp;Jiexin Zheng ,&nbsp;Bo Xu ,&nbsp;Kerui Dan ,&nbsp;Chunlei Cao ,&nbsp;Feng Sun ,&nbsp;Qiyang Zhao ,&nbsp;Yue He ,&nbsp;Jing Li ,&nbsp;Yongliang Cui ,&nbsp;Yaohai Zhang","doi":"10.1016/j.talanta.2026.129416","DOIUrl":"10.1016/j.talanta.2026.129416","url":null,"abstract":"<div><div>Cyenopyrafen is an effective pyrazole acaricide used for mite control on various crops. This study generated high-affinity monoclonal antibodies specific to cyenopyrafen and developed a high-throughput indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) validated for sensitivity, specificity, and reproducibility. The ic-ELISA demonstrated an IC₅₀ of 32.75 ng/mL and a dynamic range of 2.33–293.19 ng/mL, with negligible cross-reactivity (&lt;0.1 %) with related acaricides. Recovery rates in citrus samples were 88.18 %–105.89 %. A strong correlation (R² &gt; 0.95) was detected between the results of ic-ELISA and UPLC-MS/MS, highlighting the assay's reliability. This immunoassay offers a reliable approach for the determination of cyenopyrafen in the tested citrus and apple samples, and may serve as a basis for broader applications with potential utility in regulatory frameworks.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129416"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conformation-gated dual enzyme activity in a hemoglobin-based gadolinium single-atom catalyst for adaptive biosensing","authors":"Yifan Xing,&nbsp;Lexian Wu,&nbsp;Yiting Xu,&nbsp;Haowen Huang","doi":"10.1016/j.talanta.2026.129420","DOIUrl":"10.1016/j.talanta.2026.129420","url":null,"abstract":"<div><div>Single-atom catalysts (SACs) are known for their exceptional catalytic efficiency but suffer from structural rigidity, limiting their adaptability in biological environments. Here, we present a hemoglobin-based gadolinium single-atom catalyst (Hb-Gd SAC), in which Gd atoms are site-specifically anchored adjacent to the native heme-Fe center via biomimetic coordination. Leveraging the intrinsic flexibility of the protein scaffold, the system exhibits pH- and conformation-gated switching between two distinct enzymatic modes: peroxidase-like (POD-like) activity under acidic conditions and laccase-like activity at neutral pH. These catalytic modes are mechanistically decoupled—global protein conformation governs POD activity, while localized Gd coordination drives laccase-like function. Allosteric regulation by tartaric acid further fine-tunes this behavior, significantly enhancing performance beyond that of natural horseradish peroxidase. This dual-mode catalysis supports programmable biosensing: POD mode enables detection of thiols and acetylcholinesterase activity, while the laccase mode selectively targets dopamine. Such multimodal detection is particularly relevant for neurodegenerative disease diagnostics, exemplified by Parkinson's disease, where simultaneous monitoring of oxidative stress, cholinergic dysfunction, and dopaminergic signaling is essential. Our findings introduce a reconfigurable SAC platform that couples atomic precision with biomolecular dynamics, advancing the frontier of intelligent catalysis and adaptive biosensing technologies.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129420"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: The 23rd International Conference on flow injection analysis and related techniques (ICFIA2024).","authors":"Chalermpong Saenjum, Kate Grudpan","doi":"10.1016/j.talanta.2026.129437","DOIUrl":"10.1016/j.talanta.2026.129437","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"129437"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146043614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complementary analysis of pristine, UV-aged and extracted microplastics using single particle ICP-MS and OF2i-Raman spectroscopy","authors":"Manuel Candussi ,&nbsp;Christian Neuper ,&nbsp;Raquel Gonzalez de Vega ,&nbsp;Matthias Elinkmann ,&nbsp;Harald Fitzek ,&nbsp;Christian Hill ,&nbsp;Patrizia Marie Schmidt ,&nbsp;Svenja Seiffert ,&nbsp;David Clases","doi":"10.1016/j.talanta.2025.129339","DOIUrl":"10.1016/j.talanta.2025.129339","url":null,"abstract":"<div><div>Microplastics are of growing concern due to their ubiquity and potential risks to ecosystems and human health. Their small size, chemical diversity, and coexistence with natural colloids make comprehensive analysis difficult. Here, we evaluate two emerging single particle techniques, single particle inductively coupled plasma-mass spectrometry (SP ICP-MS) and optofluidic force induction coupled with Raman spectroscopy (OF2i-Raman), for their ability to provide complementary information at single microplastic resolution. SP ICP-MS determined carbon mass per particle and enabled detection of degradation-induced trends in size and abundance, while OF2i-Raman identified polymer type and molecular changes via optical trapping and inelastic light scattering.</div><div>OF2i-Raman is a novel method in the field of microplastic research and was therefore first benchmarked by analysing mixed suspensions of polymethylmethacrylate (PMMA), polystyrene (PS), and polyamide-6 (PA-6), as well as PA-6 in a soil extract to assess selectivity under complex matrix conditions. Subsequently, both techniques were applied in parallel to study the UV-induced degradation of PA-6 and low-density polyethylene (LDPE) as relevant industrial polymers. SP ICP-MS detected longitudinal carbon loss and relative changes in particle sizes and numbers, while OF2i-Raman revealed polymer-specific structural alterations and detected spectral fingerprints even after extended irradiation.</div><div>Together, SP ICP-MS and OF2i-Raman link elemental mass with molecular identity, providing complementary insights into microplastic degradation. While SP ICP-MS remains confined to controlled laboratory experiments due to its limited selectivity and size range, OF2i-Raman extends the analytical window to complex matrices and mixed polymer systems.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129339"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated sample preparation systems for food analysis: A mini-review","authors":"Qiong Xue ,&nbsp;Chang Liu ,&nbsp;Xiao-Hua Zhou ,&nbsp;Dezhao Kong ,&nbsp;Jinghui Zhang ,&nbsp;Wei Shen ,&nbsp;Sheng Tang","doi":"10.1016/j.talanta.2026.129395","DOIUrl":"10.1016/j.talanta.2026.129395","url":null,"abstract":"<div><div>Sample preparation has emerged as an essential component in complex food matrix analysis. Automated systems integrate precise machine operation, cutting-edge sample extraction methodologies and sophisticated detection equipment, and have been proven to effectively improve the accuracy and detection efficiency in food analysis. This review provides a mini-overview of automated sample preparation methods currently employed in the field of food analysis. These primarily encompass automated solid-phase extraction methods, microfluidics, robotic systems and integration with detection equipment. In addition, the main challenges currently faced by automated sample preparation were discussed, and suggestions for future research directions were also proposed. This review aims to serve as a reference for researchers in the field and facilitate their work on automated sample preparation methods for food analysis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129395"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced 2-butanone sensing properties of CeO2/WO3 heterojunction with multilevel channels inherited from pollen biotemplate","authors":"Chuanxuan Zhou ,&nbsp;Shaowei Li ,&nbsp;Ziyi Zhang ,&nbsp;Dousheng Han ,&nbsp;Fuchao Yang","doi":"10.1016/j.talanta.2026.129436","DOIUrl":"10.1016/j.talanta.2026.129436","url":null,"abstract":"<div><div>The ultimate optimization of natural systems in terms of “structure-function-environment adaptation” can provide inspiration for the design of advanced materials. Considering the urgent demand for efficient detection of 2-butanone in industrial safety and environmental monitoring, and the limitations of existing WO₃-based gas sensors including low response intensity and poor selectivity, this research focuses on developing modified WO₃-based composite materials with enhanced gas sensing performance using natural biological templates. In this work, an immersion process combined with calcination technology was employed to successfully replace the biosensitive scaffolds on pollen grains with gas-sensitive CeO₂/WO₃ composites. This material exhibits outstanding response to 2-butanone at 185 °C, significantly surpassing the undoped sample while maintaining high selectivity. The outstanding sensing performance is attributed to its unique hierarchical structure and the synergistic effect of the CeO₂/WO₃ heterostructure. This study provides a new perspective for us to explore low-cost gas sensors with natural biological morphologies and opens up new possibilities for expanding the applications of WO₃-based composites.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129436"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146028063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ HF-etched MXene as a novel electrode modifier for sensitive detection of bisphenol A","authors":"Ana Agurto ,&nbsp;María Pilar da Silva ,&nbsp;Luis Vázquez ,&nbsp;María Dolores Petit-Domínguez ,&nbsp;Elena Casero ,&nbsp;Carmen Quintana ,&nbsp;Elias Blanco","doi":"10.1016/j.talanta.2026.129363","DOIUrl":"10.1016/j.talanta.2026.129363","url":null,"abstract":"<div><div>This study explores the use of MXene, a relatively recent class of 2D materials, as an innovative modifier of glassy carbon (GC) electrodes for the electrochemical detection of bisphenol A (BPA), a compound of significant environmental and health concern. MXene Ti₃C₂ was synthesized via in-situ hydrofluoric acid generation using a NaF/HCl mixture that is considered a milder synthetic route in comparison with ex-situ hydrofluoric acid, and safer because it avoids the handling and storage of commercial HF. Nanomaterials obtained at various stages of MXene synthesis were morphologically characterized using scanning electron microscopy. With respect to the bare GC, MXene improved the BPA electroanalysis due to the known MXene properties such as metallic conductivity. This enhanced response was further improved when MXene was exfoliated via a delamination process. Scan rate studies proved that the BPA mechanism was governed by a diffusion-controlled process. Once MXene concentration in aqueous suspensions used to modify the GC surface was optimized, pH and differential pulse parameters were adjusted. The optimal conditions were 0.1 M phosphate buffer at pH 7 as electrolyte, pulse amplitude of 60 mV and scan rate of 20 mV/s. Sensor performance evaluation yielded a detection limit of 0.11 μM. The applicability of the sensor was supported by the analysis of real water samples. Results were corroborated by ultra-high performance liquid chromatographic analysis, demonstrating the sensor's applicability and reliability.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129363"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-acetylcysteine-functionalised a multimodal HPLC stationary phase for broad-range separations","authors":"Hayriye Aral ,&nbsp;Tarık Aral ,&nbsp;Murat Sunkur ,&nbsp;Mehmet Çolak ,&nbsp;Mesut Bağcık","doi":"10.1016/j.talanta.2026.129397","DOIUrl":"10.1016/j.talanta.2026.129397","url":null,"abstract":"<div><div>In this study, a new multimodal HPLC stationary phase derived from N-acetyl-<span>l</span>-cysteine was synthesized and comprehensively evaluated across a broad polarity spectrum. In chromatographic stationary phase design, two fundamental criteria are particularly important: a facile, low-cost synthesis and broad applicability across diverse analyte classes. The present stationary phase fulfils both requirements, as it is prepared from a commercially available starting material via a simple single-step immobilisation onto silica, providing a practical and economical synthetic route alongside wide analytical utility. The molecular architecture of the ligand, incorporating a pH-responsive carboxyl group, a hydrogen-bond-donating and -accepting amide moiety, a polarisation-sensitive sulfur atom, and hydrophobic methyl and methylene units, creates a versatile interaction environment capable of mediating hydrophobic, polar, dispersive, and weak cation-exchange processes. This multifunctional design enables the stationary phase to adapt its selectivity according to analyte structure and mobile-phase conditions, supporting both reversed-phase-like and HILIC-like retention behaviour within a single chromatographic platform. The chromatographic performance of the NAC-derived stationary phase was systematically evaluated using a wide range of chemically and biologically relevant analytes. Under HILIC conditions, the column successfully resolved 7 of the 9 highly polar nucleobases and nucleosides. In reversed-phase mode, compounds with high hydrophobicity—including 6 alkyl benzenes, 4 polycyclic aromatic hydrocarbons, 6 Sudan dyes, 8 anilines, and 7 benzoic acid derivatives—were efficiently separated. Under the same reversed-phase conditions, the stationary phase successfully resolved 10 analytes from a twelve-analyte plant growth regulator mixture and 9 sulphonamides. In addition, a 6 herbicide was also effectively separated, highlighting the multimodal selectivity of the stationary phase. Retention trends were interpreted using analyte logD and pKa values, clarifying the combined contributions of hydrophobic, polar, and weak ion-exchange interactions to overall selectivity. The N-acetyl-<span>l</span>-cysteine-based stationary phase provides a finely balanced interaction profile, enabling high separation performance for analytes spanning from extreme polarity to pronounced hydrophobicity and demonstrating broad analytical utility.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129397"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-oxidation and proximity crosslinking of G-quadruplex in G-quadruplex /hemin peroxidase system","authors":"Xiaojuan Yang ,&nbsp;Jin Zhou ,&nbsp;Dihua Shangguan","doi":"10.1016/j.talanta.2025.129344","DOIUrl":"10.1016/j.talanta.2025.129344","url":null,"abstract":"<div><div>G-quadruplex (G4)/hemin peroxidase has emerged as a robust platform for signal amplification in biosensors. While offering distinct advantages over protein enzymes, its susceptibility to inactivation often results in low apparent catalytic activity, thereby limiting broader applications. To address this, we investigated the side reactions occurring during the catalytic process of G4/hemin DNAzyme in both N-acetyl-cysteine (NAC) and H₂O₂ systems. Notably, high-molecular-weight products were observed when the hemin/G4 ratio exceeded 10. Further analysis revealed that these products primarily resulted from the self-oxidation of G4 sequences or proximity cross-linking with hemin fractions, substrates, and coexisting DNA sequences. Our findings provide critical insights into the inactivation mechanism and offer guidance for enhancing the catalytic efficiency of G4-based biosensors.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"302 ","pages":"Article 129344"},"PeriodicalIF":6.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}