IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-01-12 DOI: 10.1016/j.talanta.2026.129396

Yuhang Chen , Bill Boggess , Junyan Yang , Nicholas E. Manicke , Merlin L. Bruening

Erythropoietin (EPO) is a glycoprotein hormone used to treat chronic anemia. Glycans at the three N-glycosylation sites strongly influence EPO's stability and bioactivity, so accurate quantitation of EPO and analysis of its glycosylation patterns are critical during production and subsequent processing. This work demonstrates a rapid workflow for EPO capture, quantitation, and glycan profiling. Newly developed porous membranes containing affinity peptides selectively capture EPO from cell culture supernatants. In a 96-well-plate format, captured EPO was labeled with a fluorescent antibody to enable quantitation in a 10-min assay with an average coefficient of variation of 12 %. Elution of captured EPO from a 2-cm glass-fiber membrane combined with tryptic membranes enabled efficient purification and digestion for liquid chromatography–tandem mass spectrometry (LC–MS/MS) glycan analysis. Without purification, conventional LC-MS/MS identification of EPO N-glycans in Chinese hamster ovary (CHO) cell supernatant was not possible. The rapid in-membrane digestion yielded glycan profiles comparable to those obtained from overnight in-solution digestion. Thus, the membrane-based assays provide a novel approach for rapid EPO quantitation and facilitating glycan identification.

促红细胞生成素（EPO）是一种用于治疗慢性贫血的糖蛋白激素。三个n -糖基化位点的聚糖强烈影响EPO的稳定性和生物活性，因此准确定量EPO和分析其糖基化模式在生产和后续加工过程中至关重要。这项工作展示了EPO捕获、定量和聚糖分析的快速工作流程。新开发的含有亲和肽的多孔膜选择性地从细胞培养上清中捕获EPO。在96孔板格式中，捕获的EPO用荧光抗体标记，以便在10分钟的分析中定量，平均变异系数为12%。将捕获的EPO从2厘米的玻璃纤维膜和色氨酸膜结合中洗脱，实现高效的纯化和消化，用于液相色谱-串联质谱（LC-MS /MS）聚糖分析。未经纯化，无法对中国仓鼠卵巢（CHO）细胞上清液中EPO n -聚糖进行常规LC-MS/MS鉴定。快速膜内消化产生的聚糖谱与隔夜溶液内消化获得的聚糖谱相当。因此，基于膜的检测为快速定量EPO和促进聚糖鉴定提供了一种新的方法。

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引用次数: 0

Research on rapid diagnosis of human brucellosis based on serumer transform infrared spectroscopy combined with machine learning algorithms 基于血清变换红外光谱结合机器学习算法的人类布鲁氏菌病快速诊断研究

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-01-12 DOI: 10.1016/j.talanta.2026.129385

Ziyi Fang , Quan Wang , Xiangxiang Zheng , Hui Zhao , Yiwei Gong , Xiaojuan Bi , Renyong Lin , Guodong Lü

Human brucellosis is highly detrimental, with the potential to harm various organ systems and result in long-term health issues. Brucellosis is treatable in its early stages, but becomes challenging to cure in later stages and can be fatal. Thus, early diagnosis is essential for managing the disease and minimizing complications. Traditional brucellosis detection techniques suffer from being slow and inaccurate, highlighting the need for a quicker and more reliable method. This study aimed to investigate the potential of utilizing dry serum Fourier transform infrared (FTIR) spectroscopy (absorbance/attenuated total reflectance (ATR) spectrum) in conjunction with machine learning algorithms to effectively differentiate between individuals with brucellosis and healthy control subjects. In the 1700-1500 cm-1 range, the two spectra exhibit peaks that were opposite. Among the six spectral regions examined, all regions exhibits a notably robust discriminatory capability when analyzed using support vector machine (SVM)-linear, SVM-radial basis function (RBF), principal component analysis-linear discriminant analysis (PCA-LDA), decision trees (DT), k-nearest neighbors (KNN) methodologies, and Partial Least Squares Discriminant Analysis (PLS-DA). The PLS-DA algorithm, when used with the 3080–2800 cm⁻¹ region, achieved the highest classification accuracy. The serum FTIR (absorbance), paired with this algorithm, reaches 99.62 ± 0.52 %, surpassing the 98.48 ± 1.72 % accuracy of the ATR spectrum. According to this study, combining serum FTIR spectroscopy with the PLS-DA algorithm shows promising clinical applications for detecting human brucellosis.

人类布鲁氏菌病非常有害，有可能损害各种器官系统并导致长期健康问题。布鲁氏菌病在早期阶段是可以治疗的，但在后期阶段变得难以治愈，并且可能致命。因此，早期诊断对于控制疾病和减少并发症至关重要。传统的布鲁氏菌病检测技术存在缓慢和不准确的问题，这突出表明需要一种更快和更可靠的方法。本研究旨在探讨利用干血清傅里叶变换红外（FTIR）光谱（吸光度/衰减全反射（ATR）光谱）结合机器学习算法有效区分布鲁氏菌病患者和健康对照者的潜力。在1700 ~ 1500 cm-1范围内，两种光谱呈现相反的峰。在研究的六个光谱区域中，当使用支持向量机(SVM)-线性、支持向量机-径向基函数（RBF）、主成分分析-线性判别分析（PCA-LDA）、决策树（DT）、k近邻（KNN）方法和偏最小二乘判别分析（PLS-DA）进行分析时，所有区域都表现出显著的鲁棒性。PLS-DA算法在3080 ~ 2800 cm−1区域使用时，分类精度最高。与该算法配对的血清FTIR（吸光度）准确度达到99.62±0.52%，超过了ATR光谱的98.48±1.72%。根据本研究，将血清FTIR光谱与PLS-DA算法相结合，在检测人类布鲁氏菌病方面具有良好的临床应用前景。

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引用次数: 0

Rapid identification of Polygonatum kingianum processed by nine steaming and nine drying based on FT-NIR and ATR-FTIR combined with deep learning 基于FT-NIR和ATR-FTIR结合深度学习的九蒸九干黄精快速鉴别

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-01-11 DOI: 10.1016/j.talanta.2026.129390

Hui Ma , Shaobing Yang , Yuanzhong Wang

The nine-steaming and nine drying process is the traditional preparation method for Polygonatum sibiricum, involving repeated steaming and drying nine times to optimize its dual medicinal and edible value, making it the preferred technique. Though costly, unscrupulous merchants cut corners by reducing production steps to lower costs, yet the differences are nearly undetectable to the naked eye. Therefore, it is urgently necessary to establish a rapid and accurate identification method. This study employed a combination of Fourier transform near-infrared (FT-NIR) spectroscopy and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy with deep learning to identify nine processed samples of nine steaming and nine drying of Polygonatum kingianum. The results demonstrated that the residual neural network (ResNet) model based on synchronous two-dimensional correlation spectroscopy (2DCOS) images exhibited stable performance and high accuracy. Both the test set and training set achieved an accurate rate of 100 %. Subsequently, to explore the critical factors in different treated samples, we employed principal component analysis (PCA) to interpret the spectral variables and identified that the key variables were related to carbohydrates. Therefore, we analyzed the sugar metabolites of nine treatments using gas chromatography-mass spectrometry (GC-MS) and identified d-Xylulose, d-Ribose, d-Arabinose, and l-Rhamnose as the key metabolites distinguishing the ninth steaming of Polygonatum kingianum. Ultimately, the rapid identification of Polygonatum kingianum processed by the traditional “nine steaming and nine drying” method based on FTIR and deep learning was achieved, and its identification features were analyzed using metabolomics.

九蒸九干工艺是黄精的传统制备方法，反复蒸九干，优化其药用和食用双重价值，是黄精的首选工艺。尽管成本高昂，不择手段的商人通过减少生产步骤来降低成本，但差异几乎是肉眼无法察觉的。因此，迫切需要建立一种快速准确的鉴定方法。本研究采用傅里叶变换近红外（FT-NIR）光谱和衰减全反射傅里叶变换红外（ATR-FTIR）光谱结合深度学习技术，对黄精九蒸九干的9个加工样品进行了鉴定。结果表明，基于同步二维相关光谱（2DCOS）图像的残差神经网络（ResNet）模型具有稳定的性能和较高的精度。测试集和训练集的准确率都达到了100%。随后，为了探索不同处理样品的关键因素，我们采用主成分分析（PCA）对光谱变量进行解释，发现关键变量与碳水化合物有关。因此，我们采用气相色谱-质谱（GC-MS）分析了9个处理的糖代谢产物，鉴定出d-木糖糖、d-核糖、d-阿拉伯糖和l-鼠李糖是区分黄精九蒸的关键代谢产物。最终，实现了基于FTIR和深度学习的传统“九蒸九干”方法加工金黄精的快速鉴定，并利用代谢组学分析其鉴定特征。

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引用次数: 0

Validated method for overcoming nitroaromatic thermolability in unmodified GC/MS using molecular isotope dilution mass spectrometry 用分子同位素稀释质谱法在未修饰的GC/MS中克服硝基芳香性的验证方法

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-01-10 DOI: 10.1016/j.talanta.2026.129369

Andrew J. Boggess , George S. King , Thomas L. White

Nitroaromatic explosives are frequently detected in soils, sediments, and other environmental samples. Accurate GC/MS quantitation of these thermally labile compounds is a challenge for laboratories that cannot install cold-injection hardware or alter standard instrument configurations (e.g., radiological, hazardous-waste, and many forensic labs). This work presents a validated molecular isotope dilution (m-IDMS) method that both corrects for thermally induced degradation and accounts for deuterium isotope effects on fragmentation by leveraging diverse methodologies and standardized instrumentation for 2-nitrotoluene (2-NT), 2,6-dinitrotoluene (2,6-DNT), and 2,4,6-trinitrotoluene (2,4,6-TNT). Method validation incorporated laboratory prepared spikes, certified reference materials (CRM), and third-party proficiency tests. The CRM and proficiency tests returned acceptable values for all reported nitroaromatic compounds in soil, overlapping with the certified values (95 % CI). Validation achieved parts-per-billion limits of detection, linearity across 3 orders-of-magnitude, 7.8 % (95 % CI) relative mean uncertainty, and no statistically significant bias across the linear range. Compared to calibration curve quantitation, m-IDMS improved sensitivity and uncertainty by 7x and 3x (respectively), expanded the linear range by two orders of magnitude, and decreased mean percent error across the linear range by 2.5x. An isotope effect of 6.46 ± 1.1 (95 % CI) was observed and accounted for in the M+ fragmentation of deuterated 2,6-dinitrotoluene. This work presents an efficient, optimized, and validated method to accurately quantitate thermolabile analytes in constrained operational environments without instrument modification.

硝基芳香炸药经常在土壤、沉积物和其他环境样品中检测到。对于不能安装冷注射硬件或改变标准仪器配置的实验室（例如，放射性、危险废物和许多法医实验室）来说，对这些热不稳定化合物进行准确的GC/MS定量是一个挑战。本研究提出了一种经过验证的分子同位素稀释（m-IDMS）方法，该方法利用不同的方法和标准化仪器对2-硝基甲苯（2- nt）、2,6-二硝基甲苯（2,6- dnt）和2,4,6-三硝基甲苯（2,4,6- tnt）进行了校正，并解释了氘同位素对碎片化的影响。方法验证包括实验室制备的峰值、认证标准物质（CRM）和第三方能力测试。CRM和熟练度测试返回土壤中所有报告的硝基芳香族化合物的可接受值，与认证值重叠（95% CI）。验证达到了十亿分之一的检测限，线性度超过3个数量级，相对平均不确定性为7.8% (95% CI)，并且在线性范围内没有统计学上显著的偏差。与校准曲线定量相比，m-IDMS的灵敏度和不确定度分别提高了7倍和3倍，线性范围扩大了2个数量级，线性范围内的平均百分比误差降低了2.5倍。在氘化2,6-二硝基甲苯的M+碎裂中观察到并解释了6.46±1.1 （95% CI）的同位素效应。这项工作提出了一种有效的、优化的、经过验证的方法，可以在受限的操作环境中准确地定量热稳定性分析物，而无需修改仪器。

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引用次数: 0

Electrochemical biosensor microchip platform for rapid whole-blood chloride ions quantification in CKD point-of-care monitoring 电化学生物传感器微芯片平台快速全血氯离子定量CKD护理点监测

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-01-10 DOI: 10.1016/j.talanta.2025.129320

Chia-Chun Lee , Song-Yu Lu , Cheng-Xue Yu , Chin-Chung Tseng , Kuan-Hsun Huang , Lung-Ming Fu

Chloride ion imbalance serves as a sensitive indicator of fluid status and disease progression throughout chronic kidney dysfunction, but conventional measurements remain confined to centralized analyzers that slow clinical decision-making. An electrochemical microchip platform now enables direct quantification from only 16 μL of whole blood in approximately 2 min, without centrifugation or external pumping. During lateral transport across the paper matrix, blood cells are progressively removed, forming a stable serum zone prior to reaching the sensing interface. Within this clarified region, a silver-modified screen-printed carbon electrode produces a well-defined linear sweep voltammetry signal. Two calibration intervals provide strong linearity across physiologic and pathological ranges (1–40 mM, R² = 0.9907; 40–120 mM, R² = 0.9974). Measurements from chronic kidney disease specimens closely match indirect ion-selective electrode readings, with recoveries of 103.6 % for serum (n = 50) and 104.6 % for whole blood (n = 10). The disposable format reduces testing burden, supports near-patient electrolyte evaluation, and establishes a practical platform for future AI-assisted longitudinal renal monitoring.

氯离子失衡是慢性肾功能障碍中液体状态和疾病进展的敏感指标，但传统的测量仍然局限于集中的分析仪，这减慢了临床决策。电化学微芯片平台现在可以在大约2分钟内从16 μL的全血中直接定量，而无需离心或外部泵送。在通过纸基质的横向运输过程中，血细胞逐渐被移除，在到达传感界面之前形成一个稳定的血清区。在这个澄清的区域内，银修饰的丝网印刷碳电极产生一个明确的线性扫描伏安信号。两个校准间隔在生理和病理范围内提供强线性（1-40 mM, R2 = 0.9907; 40-120 mM, R2 = 0.9974）。慢性肾脏疾病标本的测量结果与间接离子选择电极读数密切匹配，血清（n = 50）的回收率为103.6%，全血（n = 10）的回收率为104.6%。一次性的形式减少了测试负担，支持近患者电解质评估，并为未来人工智能辅助纵向肾脏监测建立了实用平台。

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引用次数: 0

Enhanced electrochemical detection of quercetin in food samples using α-Fe2O3@700 nanoparticles modified screen printed carbon electrode α-Fe2O3@700纳米粒子修饰网印碳电极增强食品中槲皮素的电化学检测

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-01-09 DOI: 10.1016/j.talanta.2026.129357

S. Lokeswara Reddy , Raheel Akram , Songporn Thipprasert , Jaroon Jakmunee

Quercetin (Que), a potent bioactive flavonoid widely found in fruits and vegetables, is renowned for its strong antioxidant, anti-inflammatory, and antiviral properties. Accurate quantification of Que is crucial for evaluating nutritional and therapeutic benefits. In this study, a highly sensitive and selective electrochemical sensor was developed using screen-printed carbon electrodes (SPCEs) modified with calcined hematite nanoparticles (α-Fe₂O₃@700 NPs). The α-Fe₂O₃ NPs, synthesized via a simple co-precipitation method followed by calcination at 700 °C, exhibited a rice grain-like morphology that enhanced electron transport and catalytic activity. The α-Fe₂O₃@700 NPs/SPCE displayed a distinct oxidation peak (Ep) at −0.046 V and a low charge transfer resistance of 573.6 Ω/cm², confirming efficient electron transfer. The sensor achieved a broad linear range (100 nM - 1.5 mM) with a low detection limit of 12.9 nM and excellent reproducibility (RSD = 3.0 %). Recovery studies in real food samples yielded satisfactory results (89.20–104.26 %), and the sensor retained 97.3 % of its initial response after 30 days, indicating high stability. These results demonstrate that α-Fe₂O₃@700 NPs enable a robust and reliable platform for sensitive quercetin detection in complex food matrices.

槲皮素（Que）是一种广泛存在于水果和蔬菜中的强效生物活性类黄酮，以其强大的抗氧化、抗炎和抗病毒特性而闻名。准确量化Que对于评估营养和治疗益处至关重要。在这项研究中，利用煅烧的赤铁矿纳米粒子（α-Fe2O3@700 NPs）修饰的丝网印刷碳电极（spce）开发了一种高灵敏度和选择性的电化学传感器。α-Fe2O3纳米粒子通过简单共沉淀法合成，然后在700℃下煅烧，呈现出大米颗粒状的形貌，增强了电子传递和催化活性。α-Fe2O3@700 NPs/SPCE在−0.046 V时表现出明显的氧化峰（Ep）和低电荷转移电阻（573.6 Ω/cm2），证实了有效的电子转移。该传感器线性范围宽（100 nM ~ 1.5 mM），检出限低（12.9 nM），重现性好（RSD = 3.0%）。在实际食品样品中的回收率研究获得了令人满意的结果（89.20 - 104.26%），并且传感器在30天后保留了97.3%的初始响应，表明高稳定性。这些结果表明，α-Fe2O3@700 NPs为复杂食品基质中槲皮素的敏感检测提供了一个稳健可靠的平台。

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引用次数: 0

Amperometric ion-selective nanoelectrodes for bioanalytical sensing and imaging 用于生物分析传感和成像的安培离子选择纳米电极

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-01-09 DOI: 10.1016/j.talanta.2026.129380

Jiyeon Kim , Shigeru Amemiya

This review is focused on the recent applications of amperometric ion-selective nanoelectrodes as emerging electrochemical methods for bioanalytical sensing and imaging. The amperometric nanoelectrodes offer advantages over the potentiometric counterparts toward unprecedented in-vitro and in-vivo ion analysis of biological systems. The amperometric nanoelectrodes serve not only as electrochemical ion sensors based on highly selective ionophores but also as the tips of scanning electrochemical microscopy (SECM) to enable ion imaging with a spatial resolution of down to 30 nm. Moreover, the high biocompatibility and simple fabrication of ion-selective nanopipets and micropipets are attractive for in vivo bioanalysis. Specifically, we will introduce the principle of amperometric ion-selective nanoelectrodes as well as nanoscale SECM for bioanalytical sensing and imaging, respectively. Applications of amperometric ion-selective nanoelectrodes are exemplified by nanoscale SECM imaging of molecular transport at the single nuclear pore complex and chemical interactions among single bacterial cells. The powerful sensing applications of amperometric ion-selective nanoelectrodes are illustrated for the detection of carbonate generated by bacterial cells as well as both in-vitro and in-vivo detection of neurotransmitter acetylcholine.

本文综述了安培离子选择纳米电极作为生物分析传感和成像的新兴电化学方法的最新应用。在生物系统的体外和体内离子分析方面，安培纳米电极比电位电极具有前所未有的优势。安培纳米电极不仅可以作为基于高选择性离子载体的电化学离子传感器，还可以作为扫描电化学显微镜（SECM）的尖端，使空间分辨率低至30纳米的离子成像成为可能。此外，离子选择纳米管和微管的高生物相容性和简单的制造对体内生物分析具有吸引力。具体来说，我们将分别介绍用于生物分析传感和成像的安培离子选择纳米电极和纳米级SECM的原理。安培离子选择纳米电极的应用通过单核孔复合体的分子运输和单个细菌细胞之间的化学相互作用的纳米级SECM成像来举例说明。安培离子选择纳米电极在检测细菌细胞产生的碳酸盐以及体外和体内检测神经递质乙酰胆碱方面的强大传感应用。

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引用次数: 0

Silver-needle-based solid phase microextraction coupled with constant flow desorption ionization for obtaining quasi-molecular ions of 1,2-Bis(2,4,6-tribromophenoxy) ethane: Enabling accurate mass spectrometry identification 基于银针的固相微萃取与恒流解吸电离相结合，用于获得1,2-二（2,4,6-三溴苯氧基）乙烷的准分子离子：实现准确的质谱鉴定。

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-01-09 DOI: 10.1016/j.talanta.2026.129384

Shuo Gao , Xiaoxuan Han , Xiaoxu Huo , XiaoFeng Dong , Chunguang Liu , Yong Tian

Accurate mass spectrometric analysis of the emerging pollutant 1,2-bis(2,4,6-tribromophenoxy) ethane (BTBPE) remains significantly challenging due to the difficulty in generating its molecular or quasi-molecular ions. This study developed a novel technique termed silver-needle-based solid-phase microextraction constant flow desorption ionization mass spectrometry (silver-needle-based SPME-CFDI-MS) for the highly efficient ionization and detection of BTBPE. By utilizing the silver needle as both a solid-phase microextraction fiber and a corona discharge electrode, this method enables preconcentration of BTBPE from water samples, followed by its desorption, ionization, and detection via CFDI-MS. During the CFDI process, corona discharge on the silver needle produces Ag⁺, while the flowing solvent desorbs BTBPE from its surface, resulting in the formation of BTBPE quasi-molecular ions ([BTBPE + Ag]⁺). The characteristic mass and tandem mass spectra of BTBPE were subsequently acquired. Under optimized parameters, the limit of detection for BTBPE reached 21 ng L⁻¹, with a linear range from 0.10 to 3.5 μg L⁻¹ (R² > 0.9969). The intra-day (n = 8, c = 0.5 μg L⁻¹) and inter-day (n = 3 per day for 5 days, c = 1.0 μg L⁻¹) precision values were 9.2 % and 16.4 %, respectively. The method was successfully applied to detect trace BTBPE in real river water and soil samples, with spike recoveries of 80–120 % demonstrating its accuracy and practicality for environmental analysis.

由于难以生成其分子或准分子离子，对新出现的污染物1,2-二（2,4,6-三溴苯氧基）乙烷（BTBPE）进行精确的质谱分析仍然具有很大的挑战性。本研究开发了一种基于银针的固相微萃取恒流解吸电离质谱法（silver-needle-based SPME-CFDI-MS），用于高效电离和检测BTBPE。该方法利用银针作为固相微萃取纤维和电晕放电电极，实现了水样中BTBPE的预浓缩，然后进行解吸、电离和CFDI-MS检测。CFDI过程中，银针上的电晕放电产生Ag+，流动的溶剂将BTBPE从其表面解吸，形成BTBPE准分子离子（[BTBPE + Ag]+）。随后获得了BTBPE的特征质量和串联质谱。优化条件下，BTBPE的检出限为21 ng L-1，线性范围为0.10 ~ 3.5 μ L-1 （R2为0.9969）。日间（n = 8, c = 0.5 μg L-1）和日间（n = 3, d, c = 1.0 μg L-1）的精密度分别为9.2%和16.4%。该方法成功地应用于实际河流水体和土壤样品中痕量BTBPE的检测，峰值回收率为80 ~ 120%，证明了该方法在环境分析中的准确性和实用性。

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引用次数: 0

On the mechanism of Nile blue and Nile red interactions within the ion-selective membrane 离子选择膜内尼罗蓝和尼罗红相互作用机理的研究

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-01-09 DOI: 10.1016/j.talanta.2026.129389

Dorota Buczyńska, Krzysztof Maksymiuk, Agata Michalska

The Nile blue/Nile red system interactions within ion-selective membranes are investigated. A dye pair was introduced to the membrane spontaneously during contact of Nile blue aqueous solution, optionally containing Nile red. Both Nile red and Nile blue are accumulated in the membrane of typical composition, however Nile blue cations incorporation requires interaction with ion-exchanger anions: tetrakis[3,5-bis(trifluoromethyl)phenyl]borate. In the presence of Nile blue the fluorescence of Nile red is suppressed due to Fluorescence Resonant Energy Transfer (FRET) to Nile blue as well as reabsorption due to Inner Filter Effect (IFE). Because of the presence of ionophore in the membrane, Nile blue cations can be released from the membrane when primary cations bind to ionophore, as tested on model example of potassium ions. This results in fluorescence intensity increase of Nile red due to mitigation of above mentioned fluorescence suppression effects. It was observed that a ratiometric fluorimetric response, dependent on time due to diffusion limitations, increases with rising potassium ions concentration within the range from 10^−4.5 M to 10^−2.5 M, which can be explored for sensing purposes. This effect can be also used to study accumulation of preferred ions in the membrane, e.g. to follow its pretreatment. The significant contribution of both mechanisms: FRET and IFE in decrease of observed emission intensity is clearly confirmed by results of fluorescence-lifetime imaging microscopy (FLIM) studies.

研究了离子选择膜内尼罗蓝/尼罗红系统的相互作用。在接触尼罗河蓝水溶液（可选地含有尼罗河红）时，一种染料对被自发地引入到膜上。尼罗河红和尼罗河蓝都积聚在典型组成的膜中，但尼罗河蓝阳离子的掺入需要与离子交换阴离子：四[3,5-二（三氟甲基）苯基]硼酸盐相互作用。在尼罗蓝存在的情况下，尼罗红的荧光由于荧光共振能量转移（FRET）到尼罗蓝以及由于内过滤效应（IFE）的重吸收而被抑制。由于膜中存在电离层，当原生阳离子与电离层结合时，尼罗蓝阳离子可以从膜中释放出来，如钾离子的模型例子所测试的那样。这导致尼罗红的荧光强度增加，这是由于上述荧光抑制效应的缓解。在10−4.5 M至10−2.5 M范围内，由于扩散限制，比例荧光响应随钾离子浓度的增加而增加，这可以用于传感目的。该效应也可用于研究优选离子在膜中的积累，例如跟踪其预处理。荧光寿命成像显微镜（FLIM）的研究结果清楚地证实了FRET和IFE这两种机制对观测到的发射强度降低的重要贡献。

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引用次数: 0

Corrigendum to 'AgNP grafted ZnFe₂O₄ for enhanced chemicapacitive detection of atrazine' [Volume 301 (2026) 1-9/129297]. “AgNP接枝ZnFe2O4用于增强阿特拉津的化学电容检测”的勘误表[卷301(2026)1-9/129297]。

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2026-01-09 DOI: 10.1016/j.talanta.2025.129336

Pritymala Basumatary, Karen Das, Priyanka Kakoty

引用次数: 0

Talanta最新文献