Pub Date : 2024-07-14DOI: 10.1016/j.talanta.2024.126568
Hang Gong, Ganping Cai, Chunyan Chen, Feng Chen, Changqun Cai
Although molecular imprinting technology has been widely used in the construction of virus sensors, it is still a great challenge to identify subtypes viruses specifically because of their high similarity in morphology, size and structure. Here, a monoclonal molecular imprinted polymers (MIPs) sensor for recognition of H5N1 is constructed to permit the accurate distinguishing of H5N1 from other influenza A virus (IAV) subtypes. Firstly, H5N1 are immobilized on magnetic microspheres to produce H5N1-MagNPs, then the high affinity nanogel H5N1-MIPs is prepared by solid phase imprinting technique. When H5N1-MIPs is combined with MagNP-H5N1, different concentrations of H5N1 are added for competitive substitution. The quantitative detection of H5N1 is realized by the change of fluorescence intensity of supernatant. As expected, the constructed sensor shows satisfactory selectivity, and can identify the target virus from highly similar IAV subtypes, such as H1N1, H7N9 and H9N2. The sensor was highly sensitive, with a detection limit of 0.58 fM, and a selectivity factor that is comparable to that of other small MIPs sensors is achieved. In addition, the proposed sensor is cheap, with a cost of only RMB 0.08 yuan. The proposed monoclonal sensor provides a new method for the specific recognition of designated virus subtype, which is expected to be used for large-scale screening and accurate treatment of infected people.
{"title":"Construction of a monoclonal molecular imprinted sensor with high affinity for specific recognition of influenza a virus subtype.","authors":"Hang Gong, Ganping Cai, Chunyan Chen, Feng Chen, Changqun Cai","doi":"10.1016/j.talanta.2024.126568","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126568","url":null,"abstract":"<p><p>Although molecular imprinting technology has been widely used in the construction of virus sensors, it is still a great challenge to identify subtypes viruses specifically because of their high similarity in morphology, size and structure. Here, a monoclonal molecular imprinted polymers (MIPs) sensor for recognition of H5N1 is constructed to permit the accurate distinguishing of H5N1 from other influenza A virus (IAV) subtypes. Firstly, H5N1 are immobilized on magnetic microspheres to produce H5N1-MagNPs, then the high affinity nanogel H5N1-MIPs is prepared by solid phase imprinting technique. When H5N1-MIPs is combined with MagNP-H5N1, different concentrations of H5N1 are added for competitive substitution. The quantitative detection of H5N1 is realized by the change of fluorescence intensity of supernatant. As expected, the constructed sensor shows satisfactory selectivity, and can identify the target virus from highly similar IAV subtypes, such as H1N1, H7N9 and H9N2. The sensor was highly sensitive, with a detection limit of 0.58 fM, and a selectivity factor that is comparable to that of other small MIPs sensors is achieved. In addition, the proposed sensor is cheap, with a cost of only RMB 0.08 yuan. The proposed monoclonal sensor provides a new method for the specific recognition of designated virus subtype, which is expected to be used for large-scale screening and accurate treatment of infected people.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-14DOI: 10.1016/j.talanta.2024.126564
Yuhan Chen, Nan Wang, Yuntai Lv, Chenyu Zhou, Qing Liang, Xingguang Su
In this work, a novel and sensitive fluorescence sensing system for alkaline phosphatase (ALP) was constructed using a bifunctional copper metal-organic framework (Cu@MOF) nanozyme, which had excellent oxidase-mimetic activity and fluorescence properties. Owing to the presence of 2-amino-1,4-benzenedicarboxylic acid (1,4-BDC-NH2) ligand, Cu@MOF displays excellent fluorescence performance at 444 nm. Additionally, Cu2+ endows the oxidase-like activity of Cu@MOF, which could trigger p-phenylenediamine (PPD) to be oxidized to a brown product (PPDox) and quench the photoluminescence of Cu@MOF through the inner filtration effect (IFE). As the preferential affinity of ATP for Cu2+, the catalytic activity of Cu@MOF was significantly reduced once ATP was added, thus PPD could not be oxidized and fluorescence was recovered. In the presence of ALP, ATP was hydrolyzed to adenosine and Pi, which allowed Cu@MOF to regain its catalytic activity and continued to catalyze the generation of PPDox. The fluorescence of Cu@MOF was therefore weakened once again. The ALP activity was directly proportional to the degree of decrease in fluorescence intensity. Thus, this novel fluorescence sensing strategy had a linear range of 0.5-60 U/L and the limit of detection was 0.14 U/L. The established sensing method could also be used to for ALP inhibitors screening, and achieved satisfactory results in determining the level of ALP activity in human serum.
本研究利用双功能铜金属有机框架(Cu@MOF)纳米酶构建了一种新型、灵敏的碱性磷酸酶(ALP)荧光传感系统,该纳米酶具有优异的氧化酶模拟活性和荧光特性。由于 2-氨基-1,4-苯二甲酸(1,4-BDC-NH2)配体的存在,Cu@MOF 在 444 纳米波长处显示出优异的荧光性能。此外,Cu2+ 还赋予了 Cu@MOF 类似氧化酶的活性,它可以引发对苯二胺(PPD)被氧化成棕色产物(PPDox),并通过内滤效应(IFE)淬灭 Cu@MOF 的光致发光。由于 ATP 对 Cu2+ 具有优先亲和力,一旦加入 ATP,Cu@MOF 的催化活性就会明显降低,从而使 PPD 无法被氧化而恢复荧光。在 ALP 的存在下,ATP 被水解为腺苷和 Pi,这使得 Cu@MOF 重新获得催化活性,继续催化 PPDox 的生成。因此,Cu@MOF 的荧光再次减弱。ALP 活性与荧光强度的降低程度成正比。因此,这种新型荧光传感策略的线性范围为 0.5-60 U/L,检测限为 0.14 U/L。所建立的传感方法也可用于 ALP 抑制剂的筛选,并在测定人血清中的 ALP 活性水平方面取得了令人满意的结果。
{"title":"Construction of the fluorescence sensing platform with a bifunctional Cu@MOF nanozyme for determination of alkaline phosphatase and its inhibitor.","authors":"Yuhan Chen, Nan Wang, Yuntai Lv, Chenyu Zhou, Qing Liang, Xingguang Su","doi":"10.1016/j.talanta.2024.126564","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126564","url":null,"abstract":"<p><p>In this work, a novel and sensitive fluorescence sensing system for alkaline phosphatase (ALP) was constructed using a bifunctional copper metal-organic framework (Cu@MOF) nanozyme, which had excellent oxidase-mimetic activity and fluorescence properties. Owing to the presence of 2-amino-1,4-benzenedicarboxylic acid (1,4-BDC-NH<sub>2</sub>) ligand, Cu@MOF displays excellent fluorescence performance at 444 nm. Additionally, Cu<sup>2+</sup> endows the oxidase-like activity of Cu@MOF, which could trigger p-phenylenediamine (PPD) to be oxidized to a brown product (PPDox) and quench the photoluminescence of Cu@MOF through the inner filtration effect (IFE). As the preferential affinity of ATP for Cu<sup>2+</sup>, the catalytic activity of Cu@MOF was significantly reduced once ATP was added, thus PPD could not be oxidized and fluorescence was recovered. In the presence of ALP, ATP was hydrolyzed to adenosine and Pi, which allowed Cu@MOF to regain its catalytic activity and continued to catalyze the generation of PPDox. The fluorescence of Cu@MOF was therefore weakened once again. The ALP activity was directly proportional to the degree of decrease in fluorescence intensity. Thus, this novel fluorescence sensing strategy had a linear range of 0.5-60 U/L and the limit of detection was 0.14 U/L. The established sensing method could also be used to for ALP inhibitors screening, and achieved satisfactory results in determining the level of ALP activity in human serum.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-14DOI: 10.1016/j.talanta.2024.126560
Valentin Mirceski, Milivoj Lovric
A novel form of differential voltammetry is proposed, developed through the implicit anodic and cathodic current components of the experimentally accessible conventional net current measured in a voltammetric experiment. By employing basic mathematical modelling of an electrode reaction of a dissolved redox couple at a conventional, macroscopic electrode within the framework of the Butler-Volmer electrode kinetic model, the implicit anodic and cathodic current components of the net conventional current are clearly defined and can be estimated. Consequently, a novel form of differential current, calculated as the difference between anodic and cathodic implicit current components associated with a single potential of the voltammetric experiment, can be established. This differential current demonstrates remarkable characteristics in terms of electrode kinetics and analytical performance, particularly in cases involving fast, seemingly electrochemically reversible electrode processes. It holds promise to be analytically superior to the best-known differential voltammetric techniques so far (e.g., square-wave voltammetry), as well as provides a means for estimating the rate constants of very fast, apparently reversible electrode processes at macroscopic electrodes under mild experimental conditions (i.e., studied at slow potential scan rates). The practical implication of the novel methodology is significant: a simple linear sweep voltammogram of a quasi-reversible electrode reaction with unknown electrode kinetic parameters can be readily transformed into the novel type of differential voltammogram through a convolution procedure of the conventional net current, paving a new way for studying electrode processes by voltammetry.
{"title":"Genuine differential voltammetry.","authors":"Valentin Mirceski, Milivoj Lovric","doi":"10.1016/j.talanta.2024.126560","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126560","url":null,"abstract":"<p><p>A novel form of differential voltammetry is proposed, developed through the implicit anodic and cathodic current components of the experimentally accessible conventional net current measured in a voltammetric experiment. By employing basic mathematical modelling of an electrode reaction of a dissolved redox couple at a conventional, macroscopic electrode within the framework of the Butler-Volmer electrode kinetic model, the implicit anodic and cathodic current components of the net conventional current are clearly defined and can be estimated. Consequently, a novel form of differential current, calculated as the difference between anodic and cathodic implicit current components associated with a single potential of the voltammetric experiment, can be established. This differential current demonstrates remarkable characteristics in terms of electrode kinetics and analytical performance, particularly in cases involving fast, seemingly electrochemically reversible electrode processes. It holds promise to be analytically superior to the best-known differential voltammetric techniques so far (e.g., square-wave voltammetry), as well as provides a means for estimating the rate constants of very fast, apparently reversible electrode processes at macroscopic electrodes under mild experimental conditions (i.e., studied at slow potential scan rates). The practical implication of the novel methodology is significant: a simple linear sweep voltammogram of a quasi-reversible electrode reaction with unknown electrode kinetic parameters can be readily transformed into the novel type of differential voltammogram through a convolution procedure of the conventional net current, paving a new way for studying electrode processes by voltammetry.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141764759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-14DOI: 10.1016/j.talanta.2024.126572
In the past few years, the COVID-19 pandemic, caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) seriously threatens global public health security due to its high contagiousness. It remains of vital importance to develop a rapid and sensitive assay for SARS-CoV-2. In this work, we proposed a sandwich-type assay based on poly(N-isopropylacrylamide) (PNIPAM), allowing efficient detection of the SARS-CoV-2 S1 protein in the homogeneous solution. Firstly, a direct sandwich-type assay was established with a linear range of 0.2–2 μg/mL and a limit of detection (LOD) of 0.11 μg/mL, which could realize rapid detection in about 1 h. Furthermore, the sandwich-type assay coupled with rolling circle amplification (RCA) obtained an increase in sensitivity of 5.9 × 104 folds with a wide linear range of 0.01 − 100 ng/mL and a LOD of 1.88 pg/mL. The average recoveries in unpretreated saliva were 90 %–113.0 %, indicating the potential of the developed method for application in practical samples. Given the high selectivity and sensitivity of the developed method, it has a significant potential for rapid and early detection of SARS-CoV-2.
{"title":"Ultra-sensitive detection of SARS-CoV-2 S1 protein by coupling rolling circle amplification with poly(N-isopropylacrylamide)-based sandwich-type assay","authors":"","doi":"10.1016/j.talanta.2024.126572","DOIUrl":"10.1016/j.talanta.2024.126572","url":null,"abstract":"<div><p>In the past few years, the COVID-19 pandemic, caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) seriously threatens global public health security due to its high contagiousness. It remains of vital importance to develop a rapid and sensitive assay for SARS-CoV-2. In this work, we proposed a sandwich-type assay based on poly(N-isopropylacrylamide) (PNIPAM), allowing efficient detection of the SARS-CoV-2 S1 protein in the homogeneous solution. Firstly, a direct sandwich-type assay was established with a linear range of 0.2–2 μg/mL and a limit of detection (LOD) of 0.11 μg/mL, which could realize rapid detection in about 1 h. Furthermore, the sandwich-type assay coupled with rolling circle amplification (RCA) obtained an increase in sensitivity of 5.9 × 10<sup>4</sup> folds with a wide linear range of 0.01 − 100 ng/mL and a LOD of 1.88 pg/mL. The average recoveries in unpretreated saliva were 90 %–113.0 %, indicating the potential of the developed method for application in practical samples. Given the high selectivity and sensitivity of the developed method, it has a significant potential for rapid and early detection of SARS-CoV-2.</p></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141711562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-14DOI: 10.1016/j.talanta.2024.126541
Swathy S, Sonia Sam, K Girish Kumar
A polyethyleneimine capped silver nanoclusters (PEI-AgNCs) based turn-off-on fluorescence sensor has been developed to determine glutathione (GSH) effectively. The fluorescence intensity of silver nanoclusters (AgNCs) has been quenched by Cu(II) and recovered by adding GSH. The quenching of fluorescence intensity of PEI-AgNCs by Cu(II) and recovery of the emission intensity of PEI-AgNCs after the addition of GSH is supposed to be ground state adduct formation. Due to the greater affinity of Cu(II) towards GSH compared to that to PEI-AgNCs, the defragmentation of PEI-AgNCs-Cu(II) adduct occurs after the addition of GSH to the solution, resulting in the recovery of emission intensity of PEI-AgNCs. Characterisation studies of the probe have been done using FT-IR spectroscopy, XPS analysis, XRD analysis, UV-visible and Fluorescence spectrophotometry, EDX spectroscopy and TEM analysis. Different experimental parameters were optimised. Under optimised analytical conditions, the sensor showed a wide linear range for the quantification of GSH from 1.00 × 10-4 M to 3.00 × 10-6 M with a detection limit (LOD) of 8.00 × 10-7 M. Selectivity and interference studies were done in the presence of different structurally similar and coexisting species of GSH in blood. The practical utility of the proposed sensor has been validated in artificial blood serum.
{"title":"Polyethyleneimine capped silver nanoclusters based turn-off-on fluorescence sensor for the determination of glutathione.","authors":"Swathy S, Sonia Sam, K Girish Kumar","doi":"10.1016/j.talanta.2024.126541","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126541","url":null,"abstract":"<p><p>A polyethyleneimine capped silver nanoclusters (PEI-AgNCs) based turn-off-on fluorescence sensor has been developed to determine glutathione (GSH) effectively. The fluorescence intensity of silver nanoclusters (AgNCs) has been quenched by Cu(II) and recovered by adding GSH. The quenching of fluorescence intensity of PEI-AgNCs by Cu(II) and recovery of the emission intensity of PEI-AgNCs after the addition of GSH is supposed to be ground state adduct formation. Due to the greater affinity of Cu(II) towards GSH compared to that to PEI-AgNCs, the defragmentation of PEI-AgNCs-Cu(II) adduct occurs after the addition of GSH to the solution, resulting in the recovery of emission intensity of PEI-AgNCs. Characterisation studies of the probe have been done using FT-IR spectroscopy, XPS analysis, XRD analysis, UV-visible and Fluorescence spectrophotometry, EDX spectroscopy and TEM analysis. Different experimental parameters were optimised. Under optimised analytical conditions, the sensor showed a wide linear range for the quantification of GSH from 1.00 × 10<sup>-4</sup> M to 3.00 × 10<sup>-6</sup> M with a detection limit (LOD) of 8.00 × 10<sup>-7</sup> M. Selectivity and interference studies were done in the presence of different structurally similar and coexisting species of GSH in blood. The practical utility of the proposed sensor has been validated in artificial blood serum.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rapid monitoring of trace antibiotics in the field in real time is essential for environment forewarning and human health. High sensitivity and real-time on-site quantitative monitoring of antibiotic residues can be accomplished by integrating portable sensors alongside fluorescent optics to construct an intelligent sensing platform that smoothly eliminates the instability of conventional detection methods. In this study, a ratiometric fluorescence sensor for the ultrasensitive detection of pefloxacin was built employing the photoinduced electron transfer (PET) mechanism from red Eu-MOFs to Mn2+-PEF complex. A visual color change results from the photoinduced electron transfer process from manganese ions to pefloxacin weakening the ligand metal charge transfer (LMCT) process in Eu-MOFs. This enables the ultrafast visible detection of pefloxacin and produces a transient shift in visual color with a detection limit as low as 15.4 nM. For the detection of pefloxacin in water, tomato, and raw pork samples, various sensing devices based on the developed fluorescent probes exhibit good practicability and accuracy. With the development of the ratiometric fluorescence sensing probe, it is now possible to quickly and quantitatively identify pefloxacin residues in the environment, offering a new method for ensuring the safety of food and people's health.
{"title":"Ion coordination and chelation in Eu-MOFs matrices: Ultrafast fluorescence visual quantification monitoring of antibiotic residues.","authors":"Yujie Guo, Lingfei Li, Shihao Xu, Maofeng Zhang, Changlong Jiang","doi":"10.1016/j.talanta.2024.126549","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126549","url":null,"abstract":"<p><p>Rapid monitoring of trace antibiotics in the field in real time is essential for environment forewarning and human health. High sensitivity and real-time on-site quantitative monitoring of antibiotic residues can be accomplished by integrating portable sensors alongside fluorescent optics to construct an intelligent sensing platform that smoothly eliminates the instability of conventional detection methods. In this study, a ratiometric fluorescence sensor for the ultrasensitive detection of pefloxacin was built employing the photoinduced electron transfer (PET) mechanism from red Eu-MOFs to Mn<sup>2+</sup>-PEF complex. A visual color change results from the photoinduced electron transfer process from manganese ions to pefloxacin weakening the ligand metal charge transfer (LMCT) process in Eu-MOFs. This enables the ultrafast visible detection of pefloxacin and produces a transient shift in visual color with a detection limit as low as 15.4 nM. For the detection of pefloxacin in water, tomato, and raw pork samples, various sensing devices based on the developed fluorescent probes exhibit good practicability and accuracy. With the development of the ratiometric fluorescence sensing probe, it is now possible to quickly and quantitatively identify pefloxacin residues in the environment, offering a new method for ensuring the safety of food and people's health.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-14DOI: 10.1016/j.talanta.2024.126571
We develop color-encoded multicompartmental hydrogel (MH) microspheres tailored for multiplexed bioassays using a drop-based microfluidic approach. Our method involves the creation of triple emulsion drops that feature thin sacrificial oil layers separating two prepolymer phases. This configuration leads to the formation of poly(ethylene glycol) (PEG) multi-compartmental core-shell microspheres through photopolymerization, followed by the removal of the thin oil layers. The core compartments stably incorporate pigments, ensuring their retention within the hydrogel network without leakage, which facilitates reliable color encoding across varying spatial positions. Additionally, we introduce small molecule fluorescent labeling into the chemically functionalized shell compartments, achieving consistent distribution of functional components without the core's contamination. Importantly, our integrated one-pot conjugation of these color-encoded microspheres with affinity peptides enables the highly sensitive and selective detection of influenza virus antigens using a fluorescence bioassay, resulting in an especially low detection limit of 0.18 nM and 0.66 nM for influenza virus H1N1 and H5N1 antigens, respectively. This approach not only highlights the potential of our microspheres in clinical diagnostics but also paves the way for their application in a wide range of multiplexed assays.
{"title":"Color-encoded multicompartmental hydrogel microspheres for multiplexed bioassays","authors":"","doi":"10.1016/j.talanta.2024.126571","DOIUrl":"10.1016/j.talanta.2024.126571","url":null,"abstract":"<div><p>We develop color-encoded multicompartmental hydrogel (MH) microspheres tailored for multiplexed bioassays using a drop-based microfluidic approach. Our method involves the creation of triple emulsion drops that feature thin sacrificial oil layers separating two prepolymer phases. This configuration leads to the formation of poly(ethylene glycol) (PEG) multi-compartmental core-shell microspheres through photopolymerization, followed by the removal of the thin oil layers. The core compartments stably incorporate pigments, ensuring their retention within the hydrogel network without leakage, which facilitates reliable color encoding across varying spatial positions. Additionally, we introduce small molecule fluorescent labeling into the chemically functionalized shell compartments, achieving consistent distribution of functional components without the core's contamination. Importantly, our integrated one-pot conjugation of these color-encoded microspheres with affinity peptides enables the highly sensitive and selective detection of influenza virus antigens using a fluorescence bioassay, resulting in an especially low detection limit of 0.18 nM and 0.66 nM for influenza virus H1N1 and H5N1 antigens, respectively. This approach not only highlights the potential of our microspheres in clinical diagnostics but also paves the way for their application in a wide range of multiplexed assays.</p></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141715432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-14DOI: 10.1016/j.talanta.2024.126570
The amplification strategies used for self-powered biosensor based on biofuel cell (BFC-SPB) need to be further developed. Because the currently developed strategies utilized the complicated hybridization of DNA or poorly readable current signal of capacitors for amplification, which limits the practical application in public health emergencies. Here, we present a facile chemical amplification strategy for BFC-SPB. The 5-min amplification was triggered by simply adding H2O2 solution dropwise to the sensing cathode after the formation of the immune sandwich. The Ag NP of immunoprobe were oxidized to Ag(I), which can be served as the electron acceptor of the cathode. The amount of immunoprobe was positively correlated with that of the antigen, resulting in corresponding and high concentration of Ag(I) after the amplification, which enhanced the ability of the cathode as the electron acceptor. Meanwhile the glucose oxidation reaction (GOR) was performed on the bioanode modified with glucose oxidase (GOx). After assembling the bioanode and sensing cathode, the open circuit voltage of the BFC-SPB, measured by digital multimeter, distinctly rised with the elevated concentration of the antigen. To demonstrate the proof of concept, immunoglobulin G (IgG), selecting as a model analyte, was sensitively detected using this method. Result indicated that the limit of detection was 4.4 fg mL−1 (0.03 amol mL−1) in the linear range of 1 pg mL−1-10 μg mL−1. This work initiates a brand-new way of chemical amplification strategy for BFC-SPB, and offers a promising platform for practical applications.
{"title":"Ultrasensitive self-powered biosensor with facile chemical signal amplification strategy using hydrogen peroxide-triggered silver oxidation reaction","authors":"","doi":"10.1016/j.talanta.2024.126570","DOIUrl":"10.1016/j.talanta.2024.126570","url":null,"abstract":"<div><p>The amplification strategies used for self-powered biosensor based on biofuel cell (BFC-SPB) need to be further developed. Because the currently developed strategies utilized the complicated hybridization of DNA or poorly readable current signal of capacitors for amplification, which limits the practical application in public health emergencies. Here, we present a facile chemical amplification strategy for BFC-SPB. The 5-min amplification was triggered by simply adding H<sub>2</sub>O<sub>2</sub> solution dropwise to the sensing cathode after the formation of the immune sandwich. The Ag NP of immunoprobe were oxidized to Ag(I), which can be served as the electron acceptor of the cathode. The amount of immunoprobe was positively correlated with that of the antigen, resulting in corresponding and high concentration of Ag(I) after the amplification, which enhanced the ability of the cathode as the electron acceptor. Meanwhile the glucose oxidation reaction (GOR) was performed on the bioanode modified with glucose oxidase (GOx). After assembling the bioanode and sensing cathode, the open circuit voltage of the BFC-SPB, measured by digital multimeter, distinctly rised with the elevated concentration of the antigen. To demonstrate the proof of concept, immunoglobulin G (IgG), selecting as a model analyte, was sensitively detected using this method. Result indicated that the limit of detection was 4.4 fg mL<sup>−1</sup> (0.03 amol mL<sup>−1</sup>) in the linear range of 1 pg mL<sup>−1</sup>-10 μg mL<sup>−1</sup>. This work initiates a brand-new way of chemical amplification strategy for BFC-SPB, and offers a promising platform for practical applications.</p></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-14DOI: 10.1016/j.talanta.2024.126565
Lihui Yuwen, Jie Ni, Jing Liang, Xinyu Liu, Zhilong Chen, Xiao Li, Huiming Lv, Jingjing Zhang, Chunyuan Song
Bacteria infections pose a serious threat to public health, and it is urgent to develop facile and accurate detection methods. To meet the important need, a potable and high-sensitive surface enhanced Raman scattering (SERS) biosensor based on aptamer recognition and catalytic hairpin assembly (CHA) signal amplification was proposed for point-of-care detection of Staphylococcus aureus (S. aureus). The SERS biosensor contains three parts: recognition probes, SERS sensing chip, and SERS tags. The feasibility of the strategy was verified by gel electrophoresis, and the one-step test route was optimized. The bacteria SERS biosensor has a good linear relationship ranging from 10 to 107 CFU mL-1 with high sensitivity low to 5 CFU mL-1, and shows excellent specificity, uniformity, and repeatability on S. aureus identification and enumeration, which can distinguish S. aureus from other bacteria. The SERS biosensor shows a good recovery rate (95.73 %-109.65 %) for testing S. aureus spiked in milk, and has good practicability for detecting S. aureus infected mouse wound, which provides a facile and reliable approach for detection of trace bacteria in the real samples.
{"title":"Portable SERS biosensor based on aptamer-assisted catalytic hairpin assembly signal amplification for ultrasensitive detection of Staphylococcus aureus.","authors":"Lihui Yuwen, Jie Ni, Jing Liang, Xinyu Liu, Zhilong Chen, Xiao Li, Huiming Lv, Jingjing Zhang, Chunyuan Song","doi":"10.1016/j.talanta.2024.126565","DOIUrl":"https://doi.org/10.1016/j.talanta.2024.126565","url":null,"abstract":"<p><p>Bacteria infections pose a serious threat to public health, and it is urgent to develop facile and accurate detection methods. To meet the important need, a potable and high-sensitive surface enhanced Raman scattering (SERS) biosensor based on aptamer recognition and catalytic hairpin assembly (CHA) signal amplification was proposed for point-of-care detection of Staphylococcus aureus (S. aureus). The SERS biosensor contains three parts: recognition probes, SERS sensing chip, and SERS tags. The feasibility of the strategy was verified by gel electrophoresis, and the one-step test route was optimized. The bacteria SERS biosensor has a good linear relationship ranging from 10 to 10<sup>7</sup> CFU mL<sup>-1</sup> with high sensitivity low to 5 CFU mL<sup>-1</sup>, and shows excellent specificity, uniformity, and repeatability on S. aureus identification and enumeration, which can distinguish S. aureus from other bacteria. The SERS biosensor shows a good recovery rate (95.73 %-109.65 %) for testing S. aureus spiked in milk, and has good practicability for detecting S. aureus infected mouse wound, which provides a facile and reliable approach for detection of trace bacteria in the real samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}