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A novel and universal dual-channel signal amplification aptasensing platform for ultrasensitive and rapid detection of cardiac biomarkers based on the mutual regulation of bimetallic organic framework and silver nanoclusters

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2025-02-16 DOI: 10.1016/j.talanta.2025.127745

Pengfei Fan , Qianji Li , Zhengduo Zhang , Shanhong Ni , Ping Jiang , Shuhong Sun , Lei Li

Cardiac troponin I (cTnI) is a key biomarker for diagnosing myocardial infarction caused by myocardial injury. The accurate and rapid monitoring of ultralow levels of cTnI is crucial for early diagnosis and risk warning of myocardial injury. Herein, a novel dual-channel signal amplification aptasensor for cTnI detection was developed utilizing the mutual regulation of bimetallic organic framework (MOFs) and silver nanoclusters (AgNCs) with the assistance of catalytic hairpin assembly (CHA). Rationally designed triple-helix molecular switch (THMS) and two hairpin probes (HP1 and HP2) containing AgNCs and a guanine-rich DNA sequence could be adsorbed onto the surface of bimetallic Cu, Mo-MOFs, enhancing the catalytic activity and reducing the fluorescence signal. The target cTnI specifically binds to the aptamer in the THMS, releasing the signal transduction probe which triggers CHA to desorb HP1–AgNCs and HP2, thereby restoring the fluorescence and decreasing the catalytic activity as well as initiating cycling. This enables dual-channel fluorescence and colorimetric detection of cTnI. The linear fluorescence and colorimetric response ranges were 0.001–20 ng/mL with LOD of 0.48 pg/mL and 0.001–10 ng/mL with LOD of 0.69 pg/mL, respectively. The aptasensor significantly increases the detection sensitivity and reduces the time required for cTnI detection in human serum, with excellent anti-interference capability. Moreover, the aptasensor shows promise for the construction of universal dual-channel aptasensors for multiple targets by altering the aptamer in THMS.

{"title":"A novel and universal dual-channel signal amplification aptasensing platform for ultrasensitive and rapid detection of cardiac biomarkers based on the mutual regulation of bimetallic organic framework and silver nanoclusters","authors":"Pengfei Fan , Qianji Li , Zhengduo Zhang , Shanhong Ni , Ping Jiang , Shuhong Sun , Lei Li","doi":"10.1016/j.talanta.2025.127745","DOIUrl":"10.1016/j.talanta.2025.127745","url":null,"abstract":"<div><div>Cardiac troponin I (cTnI) is a key biomarker for diagnosing myocardial infarction caused by myocardial injury. The accurate and rapid monitoring of ultralow levels of cTnI is crucial for early diagnosis and risk warning of myocardial injury. Herein, a novel dual-channel signal amplification aptasensor for cTnI detection was developed utilizing the mutual regulation of bimetallic organic framework (MOFs) and silver nanoclusters (AgNCs) with the assistance of catalytic hairpin assembly (CHA). Rationally designed triple-helix molecular switch (THMS) and two hairpin probes (HP1 and HP2) containing AgNCs and a guanine-rich DNA sequence could be adsorbed onto the surface of bimetallic Cu, Mo-MOFs, enhancing the catalytic activity and reducing the fluorescence signal. The target cTnI specifically binds to the aptamer in the THMS, releasing the signal transduction probe which triggers CHA to desorb HP1–AgNCs and HP2, thereby restoring the fluorescence and decreasing the catalytic activity as well as initiating cycling. This enables dual-channel fluorescence and colorimetric detection of cTnI. The linear fluorescence and colorimetric response ranges were 0.001–20 ng/mL with LOD of 0.48 pg/mL and 0.001–10 ng/mL with LOD of 0.69 pg/mL, respectively. The aptasensor significantly increases the detection sensitivity and reduces the time required for cTnI detection in human serum, with excellent anti-interference capability. Moreover, the aptasensor shows promise for the construction of universal dual-channel aptasensors for multiple targets by altering the aptamer in THMS.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127745"},"PeriodicalIF":5.6,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isotope tracing-assisted chip-based solid-phase extraction mass spectrometry for monitoring metabolic changes and vitamin D3 regulation in cells

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2025-02-15 DOI: 10.1016/j.talanta.2025.127754

Ning Xu , Haifeng Lin , Xiaodan Ding , Peilong Wang , Jin-Ming Lin

Cellular metabolism is a dynamic and essential process, with alterations in metabolic pathways serving as hallmark features of cancer. In this study, we developed a chip-based solid-phase extraction mass spectrometry (Chip-SPE-MS) platform for high-sensitivity, high-throughput analysis of cellular metabolites and real-time tracking of metabolic fluxes. The system achieved detection limits ranging from 0.10 to 9.43 μmol/mL for various amino acids and organic acids, with excellent linearity (r ≥ 0.992). By incorporating isotope tracing, the platform enabled derivatization-free, real-time monitoring of ¹³C-labeled metabolites, such as lactic acid. Our analysis revealed significant metabolic differences between normal (L02) and cancerous (HepG2, HCT116) cells, including enhanced glycolytic activity and elevated lactate production in cancer cells. Furthermore, treatment with 1,25-dihydroxyvitamin D3 was shown to suppress glucose uptake and modulate metabolic activity in HCT116 cells, highlighting the regulatory effects of vitamin D3 on cancer metabolism. This study not only provides novel insights into the metabolic reprogramming associated with cancer but also demonstrates the potential of the Chip-SPE-MS platform as a powerful tool for real-time monitoring of dynamic metabolic processes. The findings have broad implications for cancer therapy and the study of metabolic diseases.

{"title":"Isotope tracing-assisted chip-based solid-phase extraction mass spectrometry for monitoring metabolic changes and vitamin D3 regulation in cells","authors":"Ning Xu , Haifeng Lin , Xiaodan Ding , Peilong Wang , Jin-Ming Lin","doi":"10.1016/j.talanta.2025.127754","DOIUrl":"10.1016/j.talanta.2025.127754","url":null,"abstract":"<div><div>Cellular metabolism is a dynamic and essential process, with alterations in metabolic pathways serving as hallmark features of cancer. In this study, we developed a chip-based solid-phase extraction mass spectrometry (Chip-SPE-MS) platform for high-sensitivity, high-throughput analysis of cellular metabolites and real-time tracking of metabolic fluxes. The system achieved detection limits ranging from 0.10 to 9.43 μmol/mL for various amino acids and organic acids, with excellent linearity (r ≥ 0.992). By incorporating isotope tracing, the platform enabled derivatization-free, real-time monitoring of <sup>13</sup>C-labeled metabolites, such as lactic acid. Our analysis revealed significant metabolic differences between normal (L02) and cancerous (HepG2, HCT116) cells, including enhanced glycolytic activity and elevated lactate production in cancer cells. Furthermore, treatment with 1,25-dihydroxyvitamin D3 was shown to suppress glucose uptake and modulate metabolic activity in HCT116 cells, highlighting the regulatory effects of vitamin D3 on cancer metabolism. This study not only provides novel insights into the metabolic reprogramming associated with cancer but also demonstrates the potential of the Chip-SPE-MS platform as a powerful tool for real-time monitoring of dynamic metabolic processes. The findings have broad implications for cancer therapy and the study of metabolic diseases.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127754"},"PeriodicalIF":5.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A lanthanide metal organic frameworks ratiometric fluorescence sensor for visual detection of anthrax biomarker

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2025-02-15 DOI: 10.1016/j.talanta.2025.127753

Hao Zhang , Jvxin Hao , Runnan Wang , Fanxu Meng

A novel dual-emission fluorescence probe, Eu-TDA-DHTP, was developed based on a ratiometric fluorescence-organic frameworks utilizing Eu³⁺, 2,5-Thiophenedicarboxylic acid, and 2,5-dihydroxyterephthalic acid for the ratiometric and fluorescent visual sensing of the anthrax biomarker 2,6-pyridine dicarboxylic acid (DPA). The experiments demonstrated that this material can effectively enhance dual emission for DPA detection, enabling clear visualization. The limit of detection for DPA detection using Eu-TDA-DHTP is 1.9 μmol/L in fluorometric mode and 5 μmol/L in colorimetric mode. Additionally, the preparation of the Eu-TDA-DHTP@PVP composite matrix membrane and the development of a smartphone-assisted visual assay have enhanced the convenience of DPA detection in serum and urine samples. This study presents a sensitive, efficient, and user-friendly platform for the visual monitoring of DPA, an anthrax biomarker, in real samples.

引用次数: 0

Ultrafast on-site adulteration detection and quantification in Asian black truffle using smartphone-based computer vision

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2025-02-14 DOI: 10.1016/j.talanta.2025.127743

Xiao-Zhi Wang , De-Huan Yang , Zhan-Peng Yan , Xu-Dong You , Xiao-Yue Yin , Yao Chen , Tong Wang , Hai-Long Wu , Ru-Qin Yu

Asian black truffle Tuber sinense (BT) is a premium edible fungus with medicinal value, but it is often prone to adulteration. This study aims to develop a fast, non-destructive, automatic, and intelligent method for identifying BT. A novel lightweight convolutional neural network model incorporates knowledge distillation (FastBTNet) to improve model efficiency on smartphones while maintaining higher performance. The well-trained model coupled with a fast object location technique was further employed for the absolute quantification of adulteration in BT. Results showed that FastBTNet achieved 99.0 % classification accuracy, 8.5 % root mean squared error in predicting adulteration levels, and 5.3 s for predicting 1024 samples. Additionally, Grad-CAM was used to investigate the models’ recognition mechanism, and this strategy received a perfect score in the greenness assessment. These methods were deployed in a smartphone app, “Truffle Identifier,” which enables ultrafast on-site identification of a batch of samples and assists in predicting adulteration levels.

{"title":"Ultrafast on-site adulteration detection and quantification in Asian black truffle using smartphone-based computer vision","authors":"Xiao-Zhi Wang , De-Huan Yang , Zhan-Peng Yan , Xu-Dong You , Xiao-Yue Yin , Yao Chen , Tong Wang , Hai-Long Wu , Ru-Qin Yu","doi":"10.1016/j.talanta.2025.127743","DOIUrl":"10.1016/j.talanta.2025.127743","url":null,"abstract":"<div><div>Asian black truffle <em>Tuber sinense</em> (BT) is a premium edible fungus with medicinal value, but it is often prone to adulteration. This study aims to develop a fast, non-destructive, automatic, and intelligent method for identifying BT. A novel lightweight convolutional neural network model incorporates knowledge distillation (FastBTNet) to improve model efficiency on smartphones while maintaining higher performance. The well-trained model coupled with a fast object location technique was further employed for the absolute quantification of adulteration in BT. Results showed that FastBTNet achieved 99.0 % classification accuracy, 8.5 % root mean squared error in predicting adulteration levels, and 5.3 s for predicting 1024 samples. Additionally, Grad-CAM was used to investigate the models’ recognition mechanism, and this strategy received a perfect score in the greenness assessment. These methods were deployed in a smartphone app, “<em>Truffle Identifier</em>,” which enables ultrafast on-site identification of a batch of samples and assists in predicting adulteration levels.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127743"},"PeriodicalIF":5.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An automated liquid-liquid extraction platform for high-throughput sample preparation of urinary phthalate metabolites in human biomonitoring

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2025-02-14 DOI: 10.1016/j.talanta.2025.127740

Yangxu Luo , Rong He , Lisong Zhang , Pengzhe Qin , Zhijun Bai , Rongfei Peng , Hui He , Lei Tan

Automated sample preparation reduces variation caused by human factors and improves efficiency, throughput, and reliability, making it especially important in large-scale epidemiological biomonitoring applications. In this study, we demonstrated an automated liquid-liquid extraction platform that streamlines sample preparation for human biomonitoring of urinary phthalate metabolites. This platform integrates temperature-controlled enzymatic hydrolysis, adds extraction solvents, conducts shaking extraction, performs centrifugal separation, and transfers liquids. We optimized extraction solvents for liquid-liquid extraction of urinary phthalate metabolites and compared the extraction efficiency between manual and automated methods. The analytical performance of the platform was validated and compared with those obtained by manual liquid-liquid extraction and solid-phase extraction methods. We applied the automated liquid-liquid platform for determining urinary phthalate metabolites in the human biomonitoring of 232 health participants and evaluated their association with oxidative stress levels. Urinary phthalate metabolite concentrations showed a clear declining trend with increasing age. Males had significantly higher total urinary concentrations of phthalate metabolites than females. Monobutyl phthalate was the dominant metabolite in urine samples, followed by mono-isobutyl phthalate and monoethyl phthalate, with minor gender differences observed among individual metabolites. Trend tests and Bayesian Kernel Machine Regression analysis showed a significant positive association between urinary phthalate metabolites and the oxidative stress markers 8-hydroxyguanosine and 8-hydroxy-2′-deoxyguanosine, and monobutyl phthalate was identified as the most significant metabolite for the elevated 8-hydroxy-2′-deoxyguanosine levels. The automated liquid-liquid extraction platform exhibited high efficiency and reliability in preparing urinary samples for phthalate metabolite analysis, showing great promise in large-scale sample preparation of human biomonitoring applications.

{"title":"An automated liquid-liquid extraction platform for high-throughput sample preparation of urinary phthalate metabolites in human biomonitoring","authors":"Yangxu Luo , Rong He , Lisong Zhang , Pengzhe Qin , Zhijun Bai , Rongfei Peng , Hui He , Lei Tan","doi":"10.1016/j.talanta.2025.127740","DOIUrl":"10.1016/j.talanta.2025.127740","url":null,"abstract":"<div><div>Automated sample preparation reduces variation caused by human factors and improves efficiency, throughput, and reliability, making it especially important in large-scale epidemiological biomonitoring applications. In this study, we demonstrated an automated liquid-liquid extraction platform that streamlines sample preparation for human biomonitoring of urinary phthalate metabolites. This platform integrates temperature-controlled enzymatic hydrolysis, adds extraction solvents, conducts shaking extraction, performs centrifugal separation, and transfers liquids. We optimized extraction solvents for liquid-liquid extraction of urinary phthalate metabolites and compared the extraction efficiency between manual and automated methods. The analytical performance of the platform was validated and compared with those obtained by manual liquid-liquid extraction and solid-phase extraction methods. We applied the automated liquid-liquid platform for determining urinary phthalate metabolites in the human biomonitoring of 232 health participants and evaluated their association with oxidative stress levels. Urinary phthalate metabolite concentrations showed a clear declining trend with increasing age. Males had significantly higher total urinary concentrations of phthalate metabolites than females. Monobutyl phthalate was the dominant metabolite in urine samples, followed by mono-isobutyl phthalate and monoethyl phthalate, with minor gender differences observed among individual metabolites. Trend tests and Bayesian Kernel Machine Regression analysis showed a significant positive association between urinary phthalate metabolites and the oxidative stress markers 8-hydroxyguanosine and 8-hydroxy-2′-deoxyguanosine, and monobutyl phthalate was identified as the most significant metabolite for the elevated 8-hydroxy-2′-deoxyguanosine levels. The automated liquid-liquid extraction platform exhibited high efficiency and reliability in preparing urinary samples for phthalate metabolite analysis, showing great promise in large-scale sample preparation of human biomonitoring applications.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127740"},"PeriodicalIF":5.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a dual-sensing colorimetric probe for total antioxidant capacity measurement using iron(III)-o-phenanthroline reagent

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2025-02-14 DOI: 10.1016/j.talanta.2025.127751

Ervanur Elmas , Furkan Burak Şen , Mustafa Bener , Reşat Apak

In this study, a dual-mode sensing system was developed for the determination of total antioxidant capacity (TAC) using the Fe(III)-phenanthroline (Fe(III)-phen) reagent. The first detection mechanism of the system is based on the reduction of the Fe(III)-phen reagent by antioxidants, leading to the formation of the orange-red Fe(II)-phen chelate, which is quantified by the absorbance change at 510 nm. The second mechanism exploits the oxidase-like activity of the Fe(III)-phen complex. This complex generates superoxide anion radicals that oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue-colored oxidized TMB (ox-TMB) charge-transfer complex. In the presence of antioxidants, this reaction is inhibited, resulting in a decrease in ox-TMB formation, and the absorbance change at 652 nm correlates with the TAC of the tested sample. The proposed system was successfully applied to standard antioxidants, synthetic antioxidant mixtures, and real food extracts, demonstrating its applicability and sensitivity for TAC analysis. The linear equation of the calibration graphs obtained for different trolox (TR) concentrations were found to be A₅₁₀ = 0.0221C_TR + 0.0223 (A: absorbance and C: concentration in μM) and ΔA = 0.0301C_TR + 0.0583 (ΔA: the difference of absorbance resulting from decreasing ox-TMB formation in the presence of TR, and C: concentration in μM) for the reduction-based Fe(III)-phen method and the TMB-based Fe(III)-phen method, respectively. The limits of detection (LOD) for the reduction based Fe(III)-phen method and the TMB-based Fe(III)-phen method were found to be 0.45 and 0.87 μM, respectively, for trolox. The LOD was calculated using the equation; LOD = 3 s_bl/m (s_bl: standard deviation of a blank, m: slope of the calibration line). This study presents an innovative approach by utilizing the same probe, Fe(III)-phen, through two distinct mechanisms for the simple, rapid, and sensitive determination of TAC.

{"title":"Development of a dual-sensing colorimetric probe for total antioxidant capacity measurement using iron(III)-o-phenanthroline reagent","authors":"Ervanur Elmas , Furkan Burak Şen , Mustafa Bener , Reşat Apak","doi":"10.1016/j.talanta.2025.127751","DOIUrl":"10.1016/j.talanta.2025.127751","url":null,"abstract":"<div><div>In this study, a dual-mode sensing system was developed for the determination of total antioxidant capacity (TAC) using the Fe(III)-phenanthroline (Fe(III)-phen) reagent. The first detection mechanism of the system is based on the reduction of the Fe(III)-phen reagent by antioxidants, leading to the formation of the orange-red Fe(II)-phen chelate, which is quantified by the absorbance change at 510 nm. The second mechanism exploits the oxidase-like activity of the Fe(III)-phen complex. This complex generates superoxide anion radicals that oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue-colored oxidized TMB (ox-TMB) charge-transfer complex. In the presence of antioxidants, this reaction is inhibited, resulting in a decrease in ox-TMB formation, and the absorbance change at 652 nm correlates with the TAC of the tested sample. The proposed system was successfully applied to standard antioxidants, synthetic antioxidant mixtures, and real food extracts, demonstrating its applicability and sensitivity for TAC analysis. The linear equation of the calibration graphs obtained for different trolox (TR) concentrations were found to be A<sub>510</sub> = 0.0221C<sub>TR</sub> + 0.0223 (A: absorbance and C: concentration in μM) and ΔA = 0.0301C<sub>TR</sub> + 0.0583 (ΔA: the difference of absorbance resulting from decreasing ox-TMB formation in the presence of TR, and C: concentration in μM) for the reduction-based Fe(III)-phen method and the TMB-based Fe(III)-phen method, respectively. The limits of detection (LOD) for the reduction based Fe(III)-phen method and the TMB-based Fe(III)-phen method were found to be 0.45 and 0.87 μM, respectively, for trolox. The LOD was calculated using the equation; LOD = 3 s<sub>bl</sub>/<em>m</em> (s<sub>bl</sub>: standard deviation of a blank, <em>m</em>: slope of the calibration line). This study presents an innovative approach by utilizing the same probe, Fe(III)-phen, through two distinct mechanisms for the simple, rapid, and sensitive determination of TAC.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127751"},"PeriodicalIF":5.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of nitroreductase-responsive near-infrared composite nanoprobe and its application in tumor hypoxia imaging

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2025-02-14 DOI: 10.1016/j.talanta.2025.127750

Ying Chen , Guanglian Zhou , Yitong Li , Shuxian Liu , Qianru Xu , Xiumei Liu

Hypoxia is an important feature in the development of solid tumors. Nitroreductase (NTR) is closely related to the level of hypoxia, thus NTR-responsive fluorescent probes with high selectivity and sensitivity will help to evaluate the degree of hypoxia and guide personalized treatment. In this study, a near-infrared (NIR) NTR-activated composite nanoprobe 780-pNBC@MP-B was developed for tumor hypoxia imaging. The nanoprobe has a mesoporous organosilicon nanostructure functionalized with PEG for in vivo long circulation, and encapsulates a NIR molecular probe 780-pNBC for NTR imaging, which can exhibit responsive degradation to glutathione (GSH) attributed to the disulfide bond in the nanocarrier structure. Benefiting from the hydrophilic nanocarrier, 780-pNBC@MP-B with good water dispersibility and photostability was successfully applied to visualize NTR in hypoxic cells and tumor-bearing mice. Therefore, this work provides a new tool for tumor hypoxia detection and expands the new application of mesoporous silica in the field of enzyme probes.

{"title":"Construction of nitroreductase-responsive near-infrared composite nanoprobe and its application in tumor hypoxia imaging","authors":"Ying Chen , Guanglian Zhou , Yitong Li , Shuxian Liu , Qianru Xu , Xiumei Liu","doi":"10.1016/j.talanta.2025.127750","DOIUrl":"10.1016/j.talanta.2025.127750","url":null,"abstract":"<div><div>Hypoxia is an important feature in the development of solid tumors. Nitroreductase (NTR) is closely related to the level of hypoxia, thus NTR-responsive fluorescent probes with high selectivity and sensitivity will help to evaluate the degree of hypoxia and guide personalized treatment. In this study, a near-infrared (NIR) NTR-activated composite nanoprobe 780-pNBC@MP-B was developed for tumor hypoxia imaging. The nanoprobe has a mesoporous organosilicon nanostructure functionalized with PEG for in vivo long circulation, and encapsulates a NIR molecular probe 780-pNBC for NTR imaging, which can exhibit responsive degradation to glutathione (GSH) attributed to the disulfide bond in the nanocarrier structure. Benefiting from the hydrophilic nanocarrier, 780-pNBC@MP-B with good water dispersibility and photostability was successfully applied to visualize NTR in hypoxic cells and tumor-bearing mice. Therefore, this work provides a new tool for tumor hypoxia detection and expands the new application of mesoporous silica in the field of enzyme probes.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"289 ","pages":"Article 127750"},"PeriodicalIF":5.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the promotion mechanism of the surface plasmon resonance aptasensor based on ultrathin MBene nanosheets embedded with aptamer-templated silver nanoclusters for aptasensing exosomal marker

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2025-02-14 DOI: 10.1016/j.talanta.2025.127716

Qiaojuan Jia , Meiruo Yang , Yihan He , Kaige Chen , Chuanpan Guo , Shuai Zhang , Minghua Wang , Zhihong Zhang , Linghao He

The sensitive and reliable detection of exosomes is crucial for the early diagnosis and prognosis of various diseases. This work presents a label-free surface plasmon resonance (SPR) aptasensor based on two-dimensional (2D) ultrathin cobalt boride nanosheets embedded with aptamer-templated silver nanoclusters (Apt/AgNCs@CoB) for the sensitive detection of the exosomal marker CD63. The Apt/AgNCs@CoB Schottky junction exhibited a high light absorption, which enhanced the coupling with SPR and promoted the electric field at the interface. This heterojunction facilitated strong charge transfer at the SPR sensing interface and amplified the SPR signal due to the localized SPR of the silver nanoclusters. Furthermore, the integration of aptamer strands in the Apt/AgNCs@CoB nanohybrid ensured the stable anchorage of probe molecules and high affinity for the target analyte. This integration simplified the aptasensor construction through a one-step modification of the SPR gold chip, which led to the improved sensitivity and direct detection of CD63. The proposed SPR aptasensor achieved an ultralow detection limit of 0.15 fg mL⁻¹ while maintaining an outstanding selectivity for CD63 detection. In addition, the developed SPR aptasensor demonstrated recoveries ranging from 92.6 % to 98.4 % during CD63 detection in human serum samples. This 2D Apt/AgNCs@CoB nanohybrid-based SPR aptasensor offers a novel and highly sensitive sensing platform for the detection of exosomal markers, with important potential applications in cancer diagnosis.

{"title":"Insight into the promotion mechanism of the surface plasmon resonance aptasensor based on ultrathin MBene nanosheets embedded with aptamer-templated silver nanoclusters for aptasensing exosomal marker","authors":"Qiaojuan Jia , Meiruo Yang , Yihan He , Kaige Chen , Chuanpan Guo , Shuai Zhang , Minghua Wang , Zhihong Zhang , Linghao He","doi":"10.1016/j.talanta.2025.127716","DOIUrl":"10.1016/j.talanta.2025.127716","url":null,"abstract":"<div><div>The sensitive and reliable detection of exosomes is crucial for the early diagnosis and prognosis of various diseases. This work presents a label-free surface plasmon resonance (SPR) aptasensor based on two-dimensional (2D) ultrathin cobalt boride nanosheets embedded with aptamer-templated silver nanoclusters (Apt/AgNCs@CoB) for the sensitive detection of the exosomal marker CD63. The Apt/AgNCs@CoB Schottky junction exhibited a high light absorption, which enhanced the coupling with SPR and promoted the electric field at the interface. This heterojunction facilitated strong charge transfer at the SPR sensing interface and amplified the SPR signal due to the localized SPR of the silver nanoclusters. Furthermore, the integration of aptamer strands in the Apt/AgNCs@CoB nanohybrid ensured the stable anchorage of probe molecules and high affinity for the target analyte. This integration simplified the aptasensor construction through a one-step modification of the SPR gold chip, which led to the improved sensitivity and direct detection of CD63. The proposed SPR aptasensor achieved an ultralow detection limit of 0.15 fg mL<sup>−1</sup> while maintaining an outstanding selectivity for CD63 detection. In addition, the developed SPR aptasensor demonstrated recoveries ranging from 92.6 % to 98.4 % during CD63 detection in human serum samples. This 2D Apt/AgNCs@CoB nanohybrid-based SPR aptasensor offers a novel and highly sensitive sensing platform for the detection of exosomal markers, with important potential applications in cancer diagnosis.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"288 ","pages":"Article 127716"},"PeriodicalIF":5.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D composite SERS substrate constructed by Au–Ag core-satellite NPs and polystyrene sphere for ultrasensitive ratiometric Raman detection of cotinine

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2025-02-14 DOI: 10.1016/j.talanta.2025.127742

Gao-Feng Wu, Jian Zhu , Guo-Jun Weng, Jian-Jun Li, Jun-Wu Zhao

To enhance the efficacy of Surface-enhanced Raman spectroscopy (SERS), noble metal nanoparticles (NMNPs) can be organized onto three-dimensional (3D) hierarchical nanostructures. This study involved constructing a 3D solid SERS substrate by layering Au–Ag core-satellite nanoparticle monolayer films (MF) on a polystyrene sphere (PS) array, termed Au–Ag core-satellite NPs-MF-PS. Optimizing the PS size was essential to maximize SERS substrate activity, with the 500 nm PS providing the best SERS enhancement factor of 1.03 × 10⁷ a two-fold increase over Au–Ag core-satellite NPs MF alone. Additionally, cotinine detection was improved by using a ratio between the target molecules and internal Raman signals from the SERS substrate. Compared to conventional methods relying on the target molecule's Raman signal, this ratiometric SERS method expanded the detection range from 10⁻⁸ - 10⁻¹ M to 10⁻⁹ - 10⁻¹ M and reduced the detection limit from 3.67 × 10⁻⁹ M to 1.68 × 10⁻¹⁰ M. This approach represents a novel direction in creating ultra-sensitive SERS platforms with broad applications, suggesting that ratiometric SERS could further promote SERS technology advancements.

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引用次数: 0

Inert-remodeling strategy to build bimetal-confined nitrogen-doped carbon nanozyme for colorimetric-chemiluminescent imaging dual-mode cascade enzyme sensing

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta

Pub Date : 2025-02-13 DOI: 10.1016/j.talanta.2025.127698

Kai Liu , Yuru Wang , Haibing Zhu , Feng Shi , Zijun Lai , Yan Long , Chuanli Ren , Juan Li , Zhanjun Yang

The metal-organic frameworks (MOFs)-derived nanozymes in air atmosphere have gained great attention in biosensing fields. Nevertheless, this derivative pattern may result in the destabilization of the MOF framework and the aggregation of active sites, consequently diminishing its catalytic activity. Herein, we reported an inert-remodeling strategy to build bimetal-confined nitrogen-doped carbon nanozyme for dual-mode cascade enzyme biosensing. The strategy was easily achieved by pyrolysis of MOFs (CoNi-ZIF-67 as model) precursor in argon atmosphere, leading to the formation of CoNi bimetallic nanoparticles uniformly confined nitrogen-doped carbon (CoNi–CN) nanozyme. This derivative nanozyme exhibits significantly enhanced peroxidase (POD)-like activity, which is 4 times higher than that of NiCo₂O₄ nanozyme (CoNi-ZIF-67 derivative in air atmosphere) and 54 times higher than that of CoNi-ZIF-67 precursor. The excellent POD-like activity of CoNi–CN nanozyme is ascribed to the following facts: i) integrate structure with uniformly dispersed CoNi bimetal active sites; ii) confinement effect of CoNi bimetal encapsulated in CN architecture. Integrating with glucose oxidase (GOx) to prepare cascade enzyme of CoNi–CN@GOx, colorimetric-chemiluminescent imaging sensor based on CoNi–CN@GOx cascade system was developed for glucose detection. Glucose was assayed in wide linear ranges of 0.08–15 mM (colorimetric) and 0.1–30 mM (CL imaging). This research provides a promising inert-remodeling strategy to construct high-performance nanozyme for dual mode biosensing applications.

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引用次数: 0

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