Pub Date : 2018-04-01DOI: 10.15446/REV.COLOMB.QUIM.V47N1.62240
Iván Mauricio Huérfano Barco, J. A. G. Dallos
Because of the importance of developing methodologies that allow agricultural residues analysis, a rapid screening qualitative method for the determination of pesticides residues in fruits and vegetables was validated. The methodology was based on the European QuEChERS extraction method with an additional cleaning step by gel permeation chromatography (GPC), which helped to reduce the number of matrix components in the final extract. The analysis was carried out by gas chromatography coupled to mass spectrometry with a single quadrupole analyzer. The methodology was appropriate for the qualitative analysis of 31 pesticides at their respective maximum residue limits. Consistent results were obtained with respect to a quantitative routine methodology in the analysis of real samples, hence the methodology was proven to be a good alternative for the fast analysis of these contaminants in fruits and vegetables.
{"title":"Método cualitativo rápido (screening) para la detección de residuos de plaguicidas en frutas y hortalizas","authors":"Iván Mauricio Huérfano Barco, J. A. G. Dallos","doi":"10.15446/REV.COLOMB.QUIM.V47N1.62240","DOIUrl":"https://doi.org/10.15446/REV.COLOMB.QUIM.V47N1.62240","url":null,"abstract":"Because of the importance of developing methodologies that allow agricultural residues analysis, a rapid screening qualitative method for the determination of pesticides residues in fruits and vegetables was validated. The methodology was based on the European QuEChERS extraction method with an additional cleaning step by gel permeation chromatography (GPC), which helped to reduce the number of matrix components in the final extract. The analysis was carried out by gas chromatography coupled to mass spectrometry with a single quadrupole analyzer. The methodology was appropriate for the qualitative analysis of 31 pesticides at their respective maximum residue limits. Consistent results were obtained with respect to a quantitative routine methodology in the analysis of real samples, hence the methodology was proven to be a good alternative for the fast analysis of these contaminants in fruits and vegetables.","PeriodicalId":43662,"journal":{"name":"Revista Colombiana de Quimica","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15446/REV.COLOMB.QUIM.V47N1.62240","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41830368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.15446/REV.COLOMB.QUIM.V47N1.63859
Edgar Andres Avendaño Florez, Elianna Castillo Serna, Diana Cristina Sinuco León
In the global migration test for plastic packaging intended for fatty foods, glyceryl triheptadecanoate (GTM) is used as the internal standard for the quantification of fatty acid methyl esters (FAMEs) by gas chromatography. In this work, ethyl 3-phenyl propanoate (EFP) and tetradecanoic acid (AM) were evaluated as alternative internal standards. The use of (AM) presented linear behavior (r > 0.99 and %Sb < 5%), good intermediate precision (HORRATr = 0.9), and accuracy (%R = 100.6%). In contrast, the evaluation of (EFP) demonstrated that this is not an adequate internal standard for the quantification of FAMEs. In addition, it was statistically evaluated that there are no significant differences in the calculation of the global migration from FAMEs quantified using GTM or AM as internal standards, but the use of EFP presents significant differences.
{"title":"Ensayo de migración global en empaques para alimentos: evaluación de patrones internos alternativos","authors":"Edgar Andres Avendaño Florez, Elianna Castillo Serna, Diana Cristina Sinuco León","doi":"10.15446/REV.COLOMB.QUIM.V47N1.63859","DOIUrl":"https://doi.org/10.15446/REV.COLOMB.QUIM.V47N1.63859","url":null,"abstract":"In the global migration test for plastic packaging intended for fatty foods, glyceryl triheptadecanoate (GTM) is used as the internal standard for the quantification of fatty acid methyl esters (FAMEs) by gas chromatography. In this work, ethyl 3-phenyl propanoate (EFP) and tetradecanoic acid (AM) were evaluated as alternative internal standards. The use of (AM) presented linear behavior (r > 0.99 and %Sb < 5%), good intermediate precision (HORRATr = 0.9), and accuracy (%R = 100.6%). In contrast, the evaluation of (EFP) demonstrated that this is not an adequate internal standard for the quantification of FAMEs. In addition, it was statistically evaluated that there are no significant differences in the calculation of the global migration from FAMEs quantified using GTM or AM as internal standards, but the use of EFP presents significant differences.","PeriodicalId":43662,"journal":{"name":"Revista Colombiana de Quimica","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15446/REV.COLOMB.QUIM.V47N1.63859","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67056604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.15446/REV.COLOMB.QUIM.V47N1.65375
M. Huamán
A method to obtain ursolic acid (UA) in an easy, fast, and economical way was proposed, having as raw material the medicinal plant Clinopodium revolutum from Peru. This plant is known and marketed as flor de arena or te indio. The selective recrystallization technique used was efficient due to UA crystals were obtained without the need for chromatographic purification methods neither the use of toxic solvents. The purity determined by HPLC is greater than 95%. Thus, the flor de arena becomes an important biosource of this compound.
提出了一种以秘鲁药用植物羊角草为原料，简便、快速、经济地制备熊果酸的方法。这种植物被称为flor de arena或the indio。所采用的选择性重结晶技术是高效的，因为UA晶体不需要色谱纯化方法，也不需要使用有毒溶剂。HPLC法测定其纯度大于95%。因此，地面成为该化合物的重要生物来源。
{"title":"Preparative method to obtain ursolic acid from Clinopodium revolutum","authors":"M. Huamán","doi":"10.15446/REV.COLOMB.QUIM.V47N1.65375","DOIUrl":"https://doi.org/10.15446/REV.COLOMB.QUIM.V47N1.65375","url":null,"abstract":"A method to obtain ursolic acid (UA) in an easy, fast, and economical way was proposed, having as raw material the medicinal plant Clinopodium revolutum from Peru. This plant is known and marketed as flor de arena or te indio. The selective recrystallization technique used was efficient due to UA crystals were obtained without the need for chromatographic purification methods neither the use of toxic solvents. The purity determined by HPLC is greater than 95%. Thus, the flor de arena becomes an important biosource of this compound.","PeriodicalId":43662,"journal":{"name":"Revista Colombiana de Quimica","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67057090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.15446/REV.COLOMB.QUIM.V47N1.63976
Christian Díaz Oviedo, Ariel Rodolfo Quevedo Pastor
La tiramina y la N-benciltiramina reaccionan con formaldehido para formar azaciclofanos por medio de condensaciones tipo Mannich aromaticas y reaccionan con aldehidos no enolizables para formar las respectivas bases de Schiff. En este articulo se presenta la sintesis inesperada de N-bencil-N-formiltiramina y N-bencil-N-metiltiramina por medio de reacciones de transamidacion y de transamidacion-reduccion de N-benciltiramina con N,N-dimetilformamida. Para explicar el curso de la reaccion se propuso un mecanismo que involucra la formilacion de N-benciltiramina y posterior reduccion de Leuckart-Wallach inducida por acido formico generado in situ.
{"title":"Transamidación y transamidación-reducción de N-benciltiramina con DMF","authors":"Christian Díaz Oviedo, Ariel Rodolfo Quevedo Pastor","doi":"10.15446/REV.COLOMB.QUIM.V47N1.63976","DOIUrl":"https://doi.org/10.15446/REV.COLOMB.QUIM.V47N1.63976","url":null,"abstract":"La tiramina y la N-benciltiramina reaccionan con formaldehido para formar azaciclofanos por medio de condensaciones tipo Mannich aromaticas y reaccionan con aldehidos no enolizables para formar las respectivas bases de Schiff. En este articulo se presenta la sintesis inesperada de N-bencil-N-formiltiramina y N-bencil-N-metiltiramina por medio de reacciones de transamidacion y de transamidacion-reduccion de N-benciltiramina con N,N-dimetilformamida. Para explicar el curso de la reaccion se propuso un mecanismo que involucra la formilacion de N-benciltiramina y posterior reduccion de Leuckart-Wallach inducida por acido formico generado in situ.","PeriodicalId":43662,"journal":{"name":"Revista Colombiana de Quimica","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15446/REV.COLOMB.QUIM.V47N1.63976","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67056175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.15446/REV.COLOMB.QUIM.V47N1.66281
César A. Sierra, Brian Castro Agudelo, Cristian Ochoa‐Puentes, William Rodríguez-Córdoba, A. Reiber
The synthesis of new terpyridine (Tpy) derivatives has been subject of extensive research due to its potential as functional materials for solar energy conversion, among other applications. In this contribution, the 4-([2,2':6',2''-terpyridin]-4'-yl)phenol (TpyOH) has been synthesized, characterized and studied through several methods, including X-ray crystallography and computational approaches. Single crystal X-ray structure analysis shows that TpyOH is essentially planar, with dihedral angles of about 5.03° between the central pyridinyl and the phenolic ring, and also 6.05 and 12.2° in the terpyridine moiety. In the crystal, molecules are linked by intermolecular hydrogen bonds and through π-π stacking interactions. Using a time-dependent density functional theory approach and taking into account bulk solvent effects, the absorption and fluorescence spectra of TpyOH were investigated and compared. The TD-DFT S0 → Sn and S1 → S0 transition energies are in good agreement with experimental results. The frontier molecular orbitals analysis showed that the low-energy absorption band has an intraligand charge transfer character (ICT), while the high-energy band is a common feature of π-π* transitions of the Tpy moiety. The S1→S0 emission transition also has an ICT character, with a 90% contribution from the HOMO→LUMO transitions.
{"title":"Synthesis, characterization, X-ray crystal structure and DFT calculations of 4-([2,2':6',2''-terpyridin]- 4'-yl)phenol","authors":"César A. Sierra, Brian Castro Agudelo, Cristian Ochoa‐Puentes, William Rodríguez-Córdoba, A. Reiber","doi":"10.15446/REV.COLOMB.QUIM.V47N1.66281","DOIUrl":"https://doi.org/10.15446/REV.COLOMB.QUIM.V47N1.66281","url":null,"abstract":"The synthesis of new terpyridine (Tpy) derivatives has been subject of extensive research due to its potential as functional materials for solar energy conversion, among other applications. In this contribution, the 4-([2,2':6',2''-terpyridin]-4'-yl)phenol (TpyOH) has been synthesized, characterized and studied through several methods, including X-ray crystallography and computational approaches. Single crystal X-ray structure analysis shows that TpyOH is essentially planar, with dihedral angles of about 5.03° between the central pyridinyl and the phenolic ring, and also 6.05 and 12.2° in the terpyridine moiety. In the crystal, molecules are linked by intermolecular hydrogen bonds and through π-π stacking interactions. Using a time-dependent density functional theory approach and taking into account bulk solvent effects, the absorption and fluorescence spectra of TpyOH were investigated and compared. The TD-DFT S0 → Sn and S1 → S0 transition energies are in good agreement with experimental results. The frontier molecular orbitals analysis showed that the low-energy absorption band has an intraligand charge transfer character (ICT), while the high-energy band is a common feature of π-π* transitions of the Tpy moiety. The S1→S0 emission transition also has an ICT character, with a 90% contribution from the HOMO→LUMO transitions.","PeriodicalId":43662,"journal":{"name":"Revista Colombiana de Quimica","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67056655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.15446/REV.COLOMB.QUIM.V47N1.64699
Oscar Felipe Arbeláez Pérez, Víctor Ignacio López Ríos, Aída Luz Villa Holguín, Adriana Patricia Villegas Quiceno
Bimetallic Cu-Ni catalysts were synthesized with molar ratio 2-1 in cylindrical activated carbon pellets of different diameters (0.2 cm, 0.3 cm, and 0.4 cm) and length (0.4 cm and 0.6 cm). They were evaluated in the water gas shift reaction at 330 °C and atmospheric pressure. An experimental design was developed to evaluate the effect of variables, diameter and length of the pellets, over carbon monoxide conversion. The importance of the main factors and their interactions were examined by analysis of variance (ANOVA). The results showed that both diameter and length of the pellets had significant effect on carbon monoxide conversion. The results of the catalytic activity indicated that up to 96% CO conversion was obtained at the optimum characteristic of the pellets, diameter of 0.2 cm and length of 0.6 cm.
{"title":"Diseño experimental para determinar el efecto del tamaño del pellet de catalizadores bimetálicos Cu-Ni en la reacción de desplazamiento de agua","authors":"Oscar Felipe Arbeláez Pérez, Víctor Ignacio López Ríos, Aída Luz Villa Holguín, Adriana Patricia Villegas Quiceno","doi":"10.15446/REV.COLOMB.QUIM.V47N1.64699","DOIUrl":"https://doi.org/10.15446/REV.COLOMB.QUIM.V47N1.64699","url":null,"abstract":"Bimetallic Cu-Ni catalysts were synthesized with molar ratio 2-1 in cylindrical activated carbon pellets of different diameters (0.2 cm, 0.3 cm, and 0.4 cm) and length (0.4 cm and 0.6 cm). They were evaluated in the water gas shift reaction at 330 °C and atmospheric pressure. An experimental design was developed to evaluate the effect of variables, diameter and length of the pellets, over carbon monoxide conversion. The importance of the main factors and their interactions were examined by analysis of variance (ANOVA). The results showed that both diameter and length of the pellets had significant effect on carbon monoxide conversion. The results of the catalytic activity indicated that up to 96% CO conversion was obtained at the optimum characteristic of the pellets, diameter of 0.2 cm and length of 0.6 cm.","PeriodicalId":43662,"journal":{"name":"Revista Colombiana de Quimica","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15446/REV.COLOMB.QUIM.V47N1.64699","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67057033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-01DOI: 10.15446/REV.COLOMB.QUIM.V46N3.62386
L. Román, F. Castro, D. Maurtua, C. Condori, Darwin Vivas, A. E. Bianchi, F. Paraguay-Delgado, J. Solís, M. M. Gómez
Using a prototype reactor, CuO nanoparticles (NPs) were synthetized through the precipitation method, starting from CuSO2·5H2O and Cu(CH3COO)2·H2O. The obtained NPs were characterized by XDR, FT-IR, SEM, and TEM. The antimicrobial activity of the NPs was determined by the plate diffusion method, placing 20 mg of NPs onto four nosocomial strains obtained from north Lima national hospital Intensive-Care Unit (Staphylococcus epidermidis, Aerococcus viridans, Ochrobactrum anthropic, and Micrococcus lylae). NPs characterization revealed that those synthetized from acetate (CuO–Acet) shown pure CuO phase, while those synthetized from sulphate CuO–Sulf shown two phases where CuO was the predominant one, having more than 84%. The crystal domains for CuO–Acet and CuO–Sulf were 15 and 19 nm, respectively. The inhibition halos for the studied strains were larger for CuO–Sulf NPs than CuO–Acet NPs, only Ochrobactrum anthropi displayed similar inhibition halos for both types of NPs.
{"title":"Nanopartículas de CuO y su propiedad antimicrobiana en cepas intrahospitalarias","authors":"L. Román, F. Castro, D. Maurtua, C. Condori, Darwin Vivas, A. E. Bianchi, F. Paraguay-Delgado, J. Solís, M. M. Gómez","doi":"10.15446/REV.COLOMB.QUIM.V46N3.62386","DOIUrl":"https://doi.org/10.15446/REV.COLOMB.QUIM.V46N3.62386","url":null,"abstract":"Using a prototype reactor, CuO nanoparticles (NPs) were synthetized through the precipitation method, starting from CuSO2·5H2O and Cu(CH3COO)2·H2O. The obtained NPs were characterized by XDR, FT-IR, SEM, and TEM. The antimicrobial activity of the NPs was determined by the plate diffusion method, placing 20 mg of NPs onto four nosocomial strains obtained from north Lima national hospital Intensive-Care Unit (Staphylococcus epidermidis, Aerococcus viridans, Ochrobactrum anthropic, and Micrococcus lylae). NPs characterization revealed that those synthetized from acetate (CuO–Acet) shown pure CuO phase, while those synthetized from sulphate CuO–Sulf shown two phases where CuO was the predominant one, having more than 84%. The crystal domains for CuO–Acet and CuO–Sulf were 15 and 19 nm, respectively. The inhibition halos for the studied strains were larger for CuO–Sulf NPs than CuO–Acet NPs, only Ochrobactrum anthropi displayed similar inhibition halos for both types of NPs.","PeriodicalId":43662,"journal":{"name":"Revista Colombiana de Quimica","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41543703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-01DOI: 10.15446/REV.COLOMB.QUIM.V46N3.63492
C. Clavijo, Nicolás Forero Baena, Marializ Magaly Ramírez Hernández
Recombinant proteins have become useful tools in biochemistry research. During their production, however, inclusion bodies (IB) appear, on the one hand, due to the high expression rate from the recombinant plasmids, which have high efficiency promoters, and, on the other hand, intrinsic characteristics of the expressed protein. Furhtermore, the nicotinamide/nicotinate mononucleotide adenilyl transferase (NMNAT) is a central protein in NAD(H)+ biosynthesis, an essential cofactor in cell metabolism, and in protozoon parasite has been studied. To study the NMNAT protein of these parasites, their recombinant version in E. coli has been expressed, getting a great quantity of IB as a by-product. To increase the solubility of the protein, the coding sequence of the NMNAT enzyme of Plasmodium falciparum was cloned in different expression plasmids which were subsequently transformed into E. coli BL21(DE3) expression strain. The solubility of the recombinant proteins was assessed and the one with the highest presence in the soluble fraction was subsequently purified and its enzyme activity was determined. The recombinant protein with a MBP (maltose-binding protein) tag showed an increased solubility and purity.
{"title":"Diseño y producción de diversas proteínas fusión de la nicotinamida/nicotinato mononucleótido adenilil transferasa (NMNAT) de Plasmodium falciparum","authors":"C. Clavijo, Nicolás Forero Baena, Marializ Magaly Ramírez Hernández","doi":"10.15446/REV.COLOMB.QUIM.V46N3.63492","DOIUrl":"https://doi.org/10.15446/REV.COLOMB.QUIM.V46N3.63492","url":null,"abstract":"Recombinant proteins have become useful tools in biochemistry research. During their production, however, inclusion bodies (IB) appear, on the one hand, due to the high expression rate from the recombinant plasmids, which have high efficiency promoters, and, on the other hand, intrinsic characteristics of the expressed protein. Furhtermore, the nicotinamide/nicotinate mononucleotide adenilyl transferase (NMNAT) is a central protein in NAD(H)+ biosynthesis, an essential cofactor in cell metabolism, and in protozoon parasite has been studied. To study the NMNAT protein of these parasites, their recombinant version in E. coli has been expressed, getting a great quantity of IB as a by-product. To increase the solubility of the protein, the coding sequence of the NMNAT enzyme of Plasmodium falciparum was cloned in different expression plasmids which were subsequently transformed into E. coli BL21(DE3) expression strain. The solubility of the recombinant proteins was assessed and the one with the highest presence in the soluble fraction was subsequently purified and its enzyme activity was determined. The recombinant protein with a MBP (maltose-binding protein) tag showed an increased solubility and purity.","PeriodicalId":43662,"journal":{"name":"Revista Colombiana de Quimica","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44286460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-01DOI: 10.15446/REV.COLOMB.QUIM.V46N3.61980
Melissa Barrera Tomas, Gloria Eva Tomas Chota, Patricia Sheen Cortavarría, Patricia Fuentes Bonilla, Miguel Angel Inocente Camones, J. Contreras
Compound usnic acid (1), isolated from lichen Evernia prunastri (Cajamarca-Peru) and the synthesis and characterization of its acyl-hydrazone (2), from the condensation reaction between usnic acid and isoniazid in an ethanol solution under reflux, giving an overall yield of 95%, were evaluated. Both compounds were evaluated and compared with isoniazid according to its anti-Mycobacterium tuberculosis activity based on the tetrazolium microplate assay (TEMA). Compound 1 had MIC (minimal inhibitory concentration) value of 16.0 μg/mL in each test of H37Rv (susceptible type), TB DM 97 (resistant wild type) and MDR DM 1098 (multi drug resistances type) strains. In similar tests, compound 2 MIC values were 2.0, 64.0 and 64.0 μg/mL respectively.
{"title":"Synthesis of acyl-hydrazone from usnic acid and isoniazid and its anti-Mycobacterium tuberculosis activity","authors":"Melissa Barrera Tomas, Gloria Eva Tomas Chota, Patricia Sheen Cortavarría, Patricia Fuentes Bonilla, Miguel Angel Inocente Camones, J. Contreras","doi":"10.15446/REV.COLOMB.QUIM.V46N3.61980","DOIUrl":"https://doi.org/10.15446/REV.COLOMB.QUIM.V46N3.61980","url":null,"abstract":"Compound usnic acid (1), isolated from lichen Evernia prunastri (Cajamarca-Peru) and the synthesis and characterization of its acyl-hydrazone (2), from the condensation reaction between usnic acid and isoniazid in an ethanol solution under reflux, giving an overall yield of 95%, were evaluated. Both compounds were evaluated and compared with isoniazid according to its anti-Mycobacterium tuberculosis activity based on the tetrazolium microplate assay (TEMA). Compound 1 had MIC (minimal inhibitory concentration) value of 16.0 μg/mL in each test of H37Rv (susceptible type), TB DM 97 (resistant wild type) and MDR DM 1098 (multi drug resistances type) strains. In similar tests, compound 2 MIC values were 2.0, 64.0 and 64.0 μg/mL respectively.","PeriodicalId":43662,"journal":{"name":"Revista Colombiana de Quimica","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15446/REV.COLOMB.QUIM.V46N3.61980","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46973972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-01DOI: 10.15446/REV.COLOMB.QUIM.V46N3.62963
L. T. Albarracin, O. J. Patino, J. Guzman, Neelu Begum, Timothy D McHugh, L. Cuca, M. Ávila
La tuberculosis causa miles de muertes a nivel mundial y que, actualmente, los farmacos usados no son suficientes y en ocasiones son obsoletos para su tratamiento, se hace necesaria la busqueda de nuevos compuestos que ayuden a combatirla. Por tanto, se evaluo la actividad antituberculosis de los alcaloides ocoxilonina (1), ocoteina (2), dicentrina (3) y 1,2-metilendioxi-3, 10,11-trimetoxiaporfina (4), aislados de la madera de Ocotea discolor. Las estructuras fueron identificadas por medio del analisis de los datos espectroscopicos de resonancia magnetica nuclear (NMR 1D – 1H, 13C, 2D – COSY, HSQC y HMBC), espectros de masas y comparacion con datos de la literatura. Todos los compuestos aislados demostraron actividad antituberculosa, con un rango de variacion en la concentracion minima inhibitoria entre 140 y 310 μM, siendo la ocoteina (2) la mas activo contra la cepa virulenta de Mycobacterium tuberculosis H37Rv.
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