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ISOLATION AND IDENTIFICATION OF PARASITIC NEMATODES (CUCULLNUS LONGICOLLIS) THAT INFECTS (MULLUS SURMULETUS) FISH IN THE MARINE WATERS OF SIRTE-LIBYA 分离和鉴定利比亚苏尔特海水中感染鱼类的寄生线虫(长颈线虫)
Pub Date : 2022-03-01 DOI: 10.47832/minarcongress4-1
Ahmed A. ALMASHAY
Due to the important geographical location that characterized the city of Sirte-Libya, and given the importance of fish wealth in this region and the negative impact that may occur as a result of infecting fish with parasites, and the lack of research studies on this subject in this region, so this research study was conducted to contribute to the to clarify this issue. A number of (70) fish specimens were collected from Sirte - Libyan coast facing Mediterranean Sea from January 2021 to March 2021 , and the measurements were made on them all marine fish examined externally in the laboratory, after that they were dissected by the scientific methods used and examined internally, as well as the digestive system was examined and conducted on parasites extracted by all the processes related to study. The discovered parasitic worms were carefully extracted, laboratory operations were conducted on them, and they were examined and photographed under a normal light microscope as well as an electron microscope. The results showed that (2) of these fish were infected with nematode worms of the species Cucullanus longicollis , which were extracted from the large intestines of these fish. This parasite was recorded for the first time in the marine waters of the city of Sirte-Libya.The general shape of the parasite has been described, supported by pictures. This research aims to identify the parasitic nematodes that infect Mullus surmuletus fish and to know the extent of environmental pollution in the study area and the rate of fish infestation, as well as to clarify and studying the general form of these parasitic worms under study
由于苏尔特-利比亚市具有重要的地理位置,并且考虑到该地区鱼类财富的重要性以及鱼类感染寄生虫可能产生的负面影响,以及该地区缺乏关于这一主题的研究,因此进行本研究是为了有助于澄清这一问题。从2021年1月至2021年3月,在利比亚苏尔特海岸面向地中海收集了许多(70)种鱼类标本,并对它们进行了测量。所有的海鱼都在实验室进行了外部检查,然后用科学的方法对它们进行了解剖和内部检查,并对消化系统进行了检查,并对所有与研究相关的过程中提取的寄生虫进行了检查。对发现的寄生虫进行了仔细的提取,进行了实验室操作,并在普通光学显微镜和电子显微镜下进行了检查和拍照。结果表明:(2)这些鱼感染了从这些鱼的大肠中提取的长颈Cucullanus线虫。这种寄生虫首次在利比亚苏尔特市的海水中被记录下来。寄生虫的一般形状已经被描述,并有图片支持。本研究旨在鉴定感染毛刺鱼的寄生线虫,了解研究区环境污染程度和鱼类侵染率,并对研究区寄生线虫的一般形态进行厘清和研究
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引用次数: 0
DETECTION OF PREVALENCE OF INTESTINAL PARASITES IN FRESHWATER FISH LIZA ABU FROM EUPHRATES RIVER, AL-DIWANIYAH CITY – IRAQ 伊拉克al-diwaniyah市幼发拉底河淡水鱼liza ABU肠道寄生虫流行率的检测
Pub Date : 2022-03-01 DOI: 10.47832/minarcongress4-8
I. Abbas MARHOON, Marwa Sami ALWAN
A 176 samples of freshwater fish Liza abu were fishing from Euphrates river in the AlDiwaniyah city – Iraq , to investigate of some intestinal parasite , identify the rate of infection with this parasites and then determining the relationship between temperature and prevalence of parasitic infection rates , for the period between October 2020 and March 2021. All fish examined by using laboratory methods to detected intestinal parasitic infection. The results revealed that the total infection rate of prevalence of intestinal parasites in Liza abu was 75.56%, and the results recorded presence of five different species of parasites were isolated from freshwater fish which are as follows: Three species belonging to two classes of protozoan : Sporozoa (Cryptosporidium sp. and Eimeria sp.) and one species belonged to Ciliata which is Tetrahymena sp. with an infection rate 55.68% , 35.79% and 17.61% respectively. Two species belong to parasitic helminthes : Eustrongylides sp.(Nematodes) with an infection rate 22.73%, and the other species is related to Neoechinorhynchus sp. (Acanthocephalans) with an infection rate of 44.88%. Also results showed a significant difference in infection rates during the months of the study, the highest percentage of infection was appeared during March and February which reached 93.94% and 91.67% respectively, while the infection rate decreased in January (45.0%)
在2020年10月至2021年3月期间,从伊拉克阿尔迪瓦尼耶市的幼发拉底河捕捞了176条淡水鱼样本Liza abu,以调查一些肠道寄生虫,确定这种寄生虫的感染率,然后确定温度与寄生虫感染率之间的关系。所有鱼均采用实验室方法检测肠道寄生虫感染。结果表明,鲤科鱼类肠道寄生虫总感染率为75.56%,从淡水鱼中分离出5种寄生虫,分别为孢子虫(隐孢子虫和艾美耳虫)和纤毛虫(四膜虫)2纲3种,感染率分别为55.68%、35.79%和17.61%。2种属于寄生蠕虫:Eustrongylides sp.(Nematodes),感染率为22.73%;另一种与Neoechinorhynchus sp.(棘头目)有亲缘关系,感染率为44.88%。各月份的感染率也有显著差异,3月和2月感染率最高,分别为93.94%和91.67%,1月感染率下降,为45.0%。
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引用次数: 0
DETERMINATION OF BIOFILM FORMATION GENES USING PCR TECHNIQUE FOR STAPH. SPP. ISOLATIONS FROM WOUND AND BURN INFECTIONS IN BAQUBA CITY 葡萄球菌生物膜形成基因的PCR检测。巴古拜市伤口和烧伤感染的隔离
Pub Date : 2022-03-01 DOI: 10.47832/minarcongress4-17
R. Ibrahim, Hussain K.K.AL-DULAIMY, Izdehar M. JASIM
The bacteria Staphylococcus aureus has been discovered to be a major source of community and hospital-acquired infections. The production of ica-dependent biofilms is critical in the persistence of infections in hospitalized patients. Between November 2017 &April 2018, the current study was conducted at Teaching Baquba Hospital's Bacteriology Laboratory in Baquba City and the laboratory of microbiology and polymerase chain reaction (PCR )unit in the Biology Department / College of Science/ Diyala University (2018). Materials and methods: We obtained 13(17.3%) Staph.aureus isolates from 100 clinical specimens (burns, wounds, urine, and blood) after identified them. Following by employed Congo Red Agar(CRA) and tissue culture plate method (TCP)to detect Biofilm development in isolates, as well as a PCR assay and particular primers to determine the presence of the icaA &icaD genes. The results showed ica A/D were found in 69 % (9/13) of cases, icaA gene is present at 7 (53.8%) and the icaD gene at 2(15 .3%) in Staph.aureus isolates. CRA method found biofilm generation in 6 (46%) of thirteen Staph. aureus isolates, while TCP detected biofilm creation in 10 (76%) isolates. When phenotypic approaches compared to the detection of the icaA and icaD genes, only 5 (71%) of the icaA genes were found to be positive by TCP, while only 2 (1% ) of the icaD genes were found to be positive by TCP. In short: The findings show the significance of S. aureus' virulence factors in clinical samples for the icaA and icaD genes and the phenotypic biofilm formation variety. The creation of in vitro slime using the CRA approach is not necessarily consistent even when the icaA and icaD genes exist. Although certain isolates lack the genes icaA & icaD, the ability to generate biofilms highlights the importance of the further gene research, and the absence of the icaA and icaD genes, the capability from certain isolates to create biopolymes emphasises the need for continuous genetic study into icas caused by variations in the number of genes associated with biofilms. When comparing phenotypic techniques, TCP is still the best tool for the screening of biofilms. The aim of this research though is that the biofilm forming potential should be actually linked to the presence of icaA and icaD genes in S. aureus isolates
金黄色葡萄球菌已被发现是社区和医院获得性感染的主要来源。ica依赖性生物膜的产生对于住院患者感染的持续存在至关重要。本研究于2017年11月至2018年4月在巴古巴市巴古巴医院细菌学实验室和迪亚拉大学生物系/理学院/微生物学和聚合酶链反应(PCR)单元实验室(2018年)进行。材料和方法:获得13株(17.3%)葡萄球菌。经鉴定的100份临床标本(烧伤、伤口、尿液和血液)中分离出金黄色葡萄球菌。随后采用刚果红琼脂(CRA)和组织培养平板法(TCP)检测分离物的生物膜发育,并采用PCR试验和特定引物确定icaA和icad基因的存在。结果显示,葡萄球菌中有69%(9/13)的病例存在icaA /D基因,有7例(53.8%)存在icaA基因,有2例(15.3%)存在icaD基因。葡萄球菌分离株。CRA法发现13株葡萄球菌中有6株(46%)产生生物膜。而TCP在10株(76%)分离物中检测到生物膜形成。与icaA和icaD基因的检测相比,只有5个(71%)icaA基因被TCP检测为阳性,而只有2个(1%)icaD基因被TCP检测为阳性。总之:研究结果表明临床样品中金黄色葡萄球菌毒力因子对icaA和icaD基因及表型生物膜形成变化的意义。即使存在icaA和icaD基因,使用CRA方法产生体外黏液也不一定一致。虽然某些分离株缺乏icaA和icaD基因,但产生生物膜的能力突出了进一步基因研究的重要性,而icaA和icaD基因的缺失,某些分离株产生生物聚合物的能力强调了对由与生物膜相关的基因数量变化引起的icas进行持续遗传研究的必要性。当比较表型技术时,TCP仍然是筛选生物膜的最佳工具。这项研究的目的是,生物膜形成的潜力实际上应该与金黄色葡萄球菌分离株中icaA和icaD基因的存在有关
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引用次数: 0
DETERMINATION OF THE ACTUAL INTENSITY BY CORRECTION OF THE EMISSION SPECTRUM LINES OF HEAVY METALS CONTAINED IN CRUDE OIL USING LASER INDUCED PLASMA –TECHNIQUE 用激光诱导等离子体技术校正原油中重金属发射谱线测定实际强度
Pub Date : 2022-03-01 DOI: 10.47832/minarcongress4-6
Ahmed Ali AKBAR, Bilal Ahmed HBEEB, Abdul khalk FUZEE
Laser induced plasma spectroscopy (LIPS) is a novel technique for elemental analysis based on laser-generated plasma. In this technique, laser pulses are applied for ablation of the sample, resulting in the vaporization and ionization of sample in hot plasma which is finally analyzed by the spectrometer. The elements are identified by their unique spectral signatures. The plasma was produced using the fundamental harmonic (1064 nm) of the Nd: YAG laser and the emission spectra were recorded at 3.3 µsec. detector gate delay to study the capabilities of LIPS as a rapid tool for material analysis. LIPS method was developed for elemental analysis of the crude oil. Because of the special features of crude oil as a liquid organic material which makes the analysis process difficult and inaccurate. This difficulty is concentrated due to the effect of the self- (absorption and stimulation), so the properties of the spectral analysis lines of the laser induced plasma spectrum (LIPS) are severely affected, and thus affecting the evaluation process accuracy. In this effort, there was an ability to determine the qualitative evaluation of the various heavy metals in a crude oil sample. For the large number of these metals, three minerals (Fe, Ni and V) were identified for the purpose of clarifying the method, After determining the optimal experimental conditions to improve the sensitivity of the developed LIPS system through the optimal selection of the most important parameters. Here, an analysis method based on global reference line selection was proposed for optimal estimation of the analytic lines properties of the plasma spectrum (actual intensity Ic , optimum temperature Te , highest stability score Ss and without self-strain fs), to improve the analysis accuracy of a crude oil sample using LIPS - technical. The optimum stability score (OSS) method was used to estimate the plasma temperature based on the calculation results from the Boltzmann diagram. Furthermore, the internal reference line for each specific mineral was considered while the correction was made for the analytical lines
激光诱导等离子体光谱(LIPS)是一种基于激光等离子体的元素分析新技术。该技术利用激光脉冲对样品进行烧蚀,使样品在热等离子体中汽化和电离,最后用光谱仪对样品进行分析。这些元素通过其独特的光谱特征来识别。利用Nd: YAG激光器的基频(1064 nm)产生等离子体,并记录了3.3µsec的发射光谱。研究了LIPS作为材料分析快速工具的能力。建立了用于原油元素分析的LIPS方法。由于原油作为液态有机物的特殊特性,使得分析过程困难且不准确。这一困难主要集中在激光诱导等离子体光谱(LIPS)的自吸收和激发效应,严重影响了光谱分析线的性能,从而影响了评价过程的准确性。在这项工作中,有能力确定对原油样品中各种重金属的定性评价。在确定了最佳实验条件后,通过对最重要参数的优化选择,提高了所开发的LIPS系统的灵敏度。为了提高LIPS -技术对原油样品的分析精度,提出了一种基于全局参考谱线选择的分析方法,对等离子体光谱分析谱线特性(实际强度Ic、最适温度Te、最高稳定性评分Ss和无自应变fs)进行最优估计。根据玻尔兹曼图的计算结果,采用最优稳定性评分(OSS)法估计等离子体温度。此外,在对分析线进行校正时,还考虑了每种特定矿物的内部参考线
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引用次数: 0
DETECTION OF SOME VIRULENCE FACTORS AND ANTIBIOTICS RESISTANCE OF KLEBSIELLA PNEUMONIAE 肺炎克雷伯菌部分毒力因子及抗生素耐药性检测
Pub Date : 2022-03-01 DOI: 10.47832/minarcongress4-9
Fatima Rammadan ABDUL, Ihsan Ali RAHEEEM, Alaa Laebi ABDULLAH, Batool Abd Al Ameer BAQER
Background: Infections of Klebsiella pneumoniae can include; diarrhea, septicemia, pneumonia, urinary tract infection and infections of soft tissues. Many factors are donated to K. pneumoniae pathogenicity particularly production of enzymes and formation of biofilm. Objective: find the relationship between the resistance of K. pneumoniae bacteria to antibiotics of quinolones and their ability to produce enzymes of beta lactamase. Materials and Methods: The Study included isolation and identification of (51) isolate of K. pneumoniae and (94) isolates of other bacteria from different clinical sources in some Baghdad hospitals. Results: The isolation and diagnosis of (51) isolates of K. pneumoniae from infection of urinary tract were 49.1%, infection of wounds were 31.3% and infection of burns were19.6%. All bacterial isolates were identified by the biochemical, cultural and microbial characteristics and confirmed by Api E20 System. I showed of β-lactamase test of Klebsiella pneumoniae revealed that (35) 68.6% isolates were positive. While 16 (31.4%) isolates were able to produce urease. Four groups of quinolones were tested by done the sensitivity test of isolates and results revealed the following percentage of resistant to Norfloxacin, Ofloxacin and ciprofloxacin consequently were (50.1%), (44.5%), (39.4%). whereas, the lower percent of resistant to Levofloxacin was (26.8%). In contrast, the βlactamase positive K. pneumoniae exhibited a high resistance in compare to isolates that negative for β-lactamase. The minimum inhibitory range concentrations of ciprofloxacin were arranged between (4-512 µgml). From isolates that resistant to Ciprofloxacin, the DNA plasmid was determined. Single plasmid bands were included in two isolates with same size and other isolates were confined free plasmid
背景:肺炎克雷伯菌感染包括;腹泻、败血症、肺炎、尿路感染和软组织感染。许多因素与肺炎克雷伯菌的致病性有关,特别是酶的产生和生物膜的形成。目的:探讨肺炎克雷伯菌对喹诺酮类抗生素的耐药性与其产生内酰胺酶的能力之间的关系。材料与方法:本研究分离鉴定了巴格达一些医院不同临床来源的51株肺炎克雷伯菌和94株其他细菌。结果:51株肺炎克雷伯菌从尿路感染、伤口感染和烧伤感染中分离和诊断的比例分别为49.1%、31.3%和19.6%。所有分离菌株经生化、培养和微生物学特性鉴定,并经Api E20系统鉴定。结果显示肺炎克雷伯菌β-内酰胺酶检测结果为(35)68.6%。16株(31.4%)菌株能产生脲酶。对4组喹诺酮类药物进行敏感性试验,对诺氟沙星、氧氟沙星和环丙沙星的耐药率分别为(50.1%)、(44.5%)、(39.4%)。而左氧氟沙星耐药率较低,为26.8%。与β-内酰胺酶阴性的肺炎克雷伯菌相比,β-内酰胺酶阳性的肺炎克雷伯菌表现出较高的耐药性。环丙沙星最小抑菌范围为(4 ~ 512µgml)。从对环丙沙星耐药的分离株中测定DNA质粒。两株大小相同的分离株均为单质粒带,其余分离株均为受限的游离质粒
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引用次数: 0
USE OF BEET ROOT FOR CULTURING GRAM NEGATIVE BACTERIA IN LABORATORY 甜菜根在实验室培养革兰氏阴性菌的应用
Pub Date : 2022-03-01 DOI: 10.47832/minarcongress4-2
Rasha Mohamed Sajet AL-OQAILI, Huda Zuheir Majeed, Firas Mohammed Sajet AL-OQAILI, Abdalkader Saeed LATIF
Microbes were grown in the laboratory for different purposes, by supporting their needs for growth .Nutrient Agar is a universal medium used for growing a wide spectrum of bacteria. The needing to make a medium which could replace the used commercial media by using materials which is available at the local market and cheap ,especially using these media at scientific search laboratories cost so much due to high needs and use. This led to think about using grains and plant roots as a substitute materials in order to prepare new media to cultivate bacteria. This study use Beet root to prepare a medium to replace the traditional ready media which used to cultivate bacteria. Detection the growth of Gram negative bacteria on Beet root Agar and Broth was done , preparation of Differential Beet Root media by adding lactose, bile salts and neutral red stain. The Differential Beet root media efficacy in growth of some Gram negative and positive pathogenic bacteria werenot significant at 0.05, if compared with the Nutrient and MacConkey media . Differentiation between lactose fermenter and non-lactose fermenter was done on differential Beet root media. It had the ability to inhibit the growth of Staphylococcus aureus in comparison with other culture media at 0.05 level. This study showed the possibility of using available , cheap and simple materials as a medium which could replace the traditional ready media , after adding nourishing and mineral salt materials which was convenient for bacteria without any change in metabolism or the morphology
微生物在实验室中培养有不同的目的,通过支持他们的生长需要。营养琼脂是一种通用的培养基,用于培养各种各样的细菌。需要使用在当地市场上可以获得的廉价材料来制造一种可以取代使用的商业媒体的媒体,特别是在科学搜索实验室使用这些媒体,由于需求和使用的高,成本很高。这导致人们想到用谷物和植物根作为替代材料,以制备新的培养基来培养细菌。本研究以甜菜根为培养基,替代传统培养基培养细菌。对甜菜根琼脂和肉汤上革兰氏阴性菌的生长情况进行检测,并通过添加乳糖、胆盐和中性红染色制备甜菜根培养基。与营养液和麦康基培养基相比,甜菜根培养基对部分革兰氏阴性和阳性致病菌的生长效果均不显著(0.05)。在不同的甜菜根培养基上进行了乳糖发酵剂和非乳糖发酵剂的鉴别。与其他培养基相比,其抑制金黄色葡萄球菌生长的能力在0.05水平上。本研究表明,在不改变细菌代谢和形态的前提下,在培养基中加入有利于细菌生长的营养盐和矿物盐等材料,可以用现有的、廉价的、简单的材料代替传统的现成培养基
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引用次数: 0
PREPARATION AND CHARACTERIZATION OF GRAPHENE OXIDE COMPLEXES FOR TRIAZOLE SCHIFF BASES 三唑席夫碱氧化石墨烯配合物的制备与表征
Pub Date : 2022-03-01 DOI: 10.47832/minarcongress4-16
Ghazwan Hassan ABDEL WAHAB, Shaima Hatem ABDULLAH, Marwan Hasan ALI, Amenah Ibrahim ALI
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引用次数: 0
ESSENTIALLY SEMIMALL QUASI-DEDEKIND MODULES AND ANTI-HOPFIAN MODULES 本质上是半小拟dedekind模和反hopfian模
Pub Date : 2022-03-01 DOI: 10.47832/minarcongress4-36
Mukdad Qaess HUSSAIN, Rehab Noori SHALLAN, Zahraa jawad KADHIM
Let V be a ring with identity and S be a unitary left Module over V. An 𝐑-Module S is essentially semismall quasi-Dedekind (ESSQD) whether Hom(S/H,S) = 0 H es S. A ring V is ESSQD if V is an ESSQD V-Module. An V -Module S is anti-hopfian if S is nonsimple and all nonzero factor Modules of S are isomorphic to S; that is for all , S Y  S . In this paper we study the relationship between ESSQD with anti-hopfian Modules and continuous Modules. We also give some examples to illustrate these relationships.
设V是一个恒等环,S是V上的一个酉左模,当hm (S/H,S) = 0 H时,𝐑-Module S本质上是一个半小拟dedekind (ESSQD),如果V是一个ESSQD V模,则环V是ESSQD。如果S是非简单的且S的所有非零因子模都同构于S,则V -模S是反hopfian的;这是给所有人的。本文研究了具有反hopfian模和连续模的ESSQD之间的关系。我们还会给出一些例子来说明这些关系。
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引用次数: 0
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