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The use of fungi in forensic science, a brief overview 真菌在法医学中的应用,简要概述
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2021-01-02 DOI: 10.1080/00085030.2020.1869390
M. C. Tranchida, S. Pelizza, L. Elíades
Abstract Fungi are increasingly being used in criminal investigations as a source of data for crime scene and can be proposed as a forensic tool. There are very few forensic investigators trained in this discipline, which justifies the small amount of published data and most justice professionals are not aware of the contribution of fungi to these investigations. A wide variety of techniques derived from biology are used by forensic investigators to study the changes in the corpse and in the ground where the corpse decomposition occurs in order to solve a crime or find clandestine burials. We review the data available on the use of mycological evidence in criminal cases and as proofs before the justice. The first uses of fungi as evidence were restricted to cases associated with poisonous or psychotropic species. Currently, the objective of forensic mycology is to date the post-mortem and post-burial intervals using information from the fungal community found on the corpse surface or in the environment associated with a clandestine burial. The use of fungi as a forensic tool in different parts of the world has aroused the interest of forensic investigators, such as the members of the different Law Enforcements who deal with solving criminal cases.
摘要真菌越来越多地被用于刑事调查,作为犯罪现场的数据来源,并可被提议作为一种法医工具。很少有受过这一学科培训的法医调查员,这证明了公布的少量数据是合理的,大多数司法专业人员都不知道真菌对这些调查的贡献。法医调查人员使用来自生物学的各种技术来研究尸体和尸体分解发生地的变化,以破案或寻找秘密埋葬处。我们审查了在刑事案件中使用真菌学证据以及作为司法证据的可用数据。真菌作为证据的首次使用仅限于与有毒或精神物种有关的案件。目前,法医真菌学的目标是利用尸体表面或与秘密埋葬有关的环境中发现的真菌群落的信息,确定死后和埋葬后的间隔时间。真菌作为法医工具在世界各地的使用引起了法医调查人员的兴趣,例如负责破获刑事案件的不同执法部门的成员。
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引用次数: 2
Validity of Willems age estimation method in children’s & adolescents’ of Dakshina Kannada Region, India Willems年龄估计方法在印度达克什那邦邦儿童和青少年中的有效性
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2021-01-02 DOI: 10.1080/00085030.2020.1858588
N. Garg, Abhinav Kathuria, Srikant N, Nandita Kp, Shweta Yellapurkar, N. Jose, J. Ahmed, V. Kulkarni
Abstract Willems gender-specific and non-specific maturity scores derive the age of the individual with acceptable error margins in the Belgian Population. This needs to be validated in various subpopulations to enable its use in age estimation. Our aim was to test the validity of both Willems’s I & II maturity scores to estimate dental age in children and adolescents (aged 5–20 yrs) in Dakshina Kannada population in India. Seven teeth of mandibular left quadrant from 800 Orthopantomograms (Male:Female::372:428) of individuals aged 5–20 years were staged using Demirjian’s method and substituted by the Willems maturity scores to derive the age. The chronological and the predicted age of the individuals were correlated to derive the mean absolute error of age estimation in male, female and total. The mean absolute error of estimation of age in males, females, and total of Willems I and II techniques were 1.11, 1.04, 1.07, 1.15, 1.04 and 1.09 years respectively. The gender nonspecific scores predominantly underestimated the age. The Willems I technique showed an underestimation of age in females and over-estimation of age in males (p < 0.001). The present study validates Willems method of age estimation in Dakshina Kannada population, showing a low error rate of ∼1 year.
摘要Willems性别特异性和非特异性成熟度评分得出了比利时人群中具有可接受误差范围的个体的年龄。这需要在不同的亚群中进行验证,以使其能够用于年龄估计。我们的目的是测试Willems I和II成熟度评分的有效性,以估计印度Dakshina Kannada人口中儿童和青少年(5-20岁)的牙科年龄。800个5-20岁个体的正颌图(男:女:372:428)中下颌左象限的7颗牙齿 使用Demirjian的方法对年进行分期,并用Willems成熟度分数代替,得出年龄。将个体的时间和预测年龄相关,得出男性、女性和总年龄估计的平均绝对误差。Willems I和II技术中男性、女性和总年龄估计的平均绝对误差分别为1.11、1.04、1.07、1.15、1.04和1.09 年。性别特异性评分主要低估了年龄。Willems I技术显示女性低估了年龄,男性高估了年龄(p < 0.001)。本研究验证了在Dakshina-Kannada人群中进行年龄估计的Willems方法,显示出~1的低错误率 年
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引用次数: 0
Decomposition process and arthropod succession on pig carcasses in Quebec (Canada) 加拿大魁北克地区猪尸体的分解过程及节肢动物演替
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2020-10-06 DOI: 10.1080/00085030.2020.1820799
Julie-Éléonore Maisonhaute, S. Forbes
Abstract Necrophagous arthropods are essential ecosystem decomposers and are important indicator species in the discipline of forensic entomology. Studies around the world have evaluated the succession of arthropods on animal carcasses, but no published data are available for the province of Quebec (Canada). The objective of this study was to evaluate the diversity of arthropods associated with pig carcasses in Quebec, and to determine the colonization sequence of the different families/species throughout the decomposition process. Three pig carcasses were deposited in a small woodland area in Trois-Rivières, in mid-June 2019. The decomposition process and the arthropod community were studied until the beginning of November (first snowfall) by visual observation, and sample collection on the carcasses, using an entomological net and pitfall traps. Five species of blow fly (Diptera: Calliphoridae) were found to lay eggs on the carcasses: Calliphora livida, C. vomitoria, Lucilia illustris, Phormia regina, and Protophormia terraenovae. Eggs/larvae of the first three species were recorded during the first week only (fresh stage-beginning of active decay), while the other two species were recorded throughout the decomposition process until the dry remains stage. Coleoptera were observed from day 0 to day 141 (end of experiment). Some families/species were associated with early stages of decomposition (e.g. Necrophila americana, Silphidae), while others were only found during the dry remains stage (e.g. Carnicops pumilio, Histeridae).
尸食性节肢动物是重要的生态系统分解者,是法医昆虫学领域的重要指示种。世界各地的研究已经评估了动物尸体上节肢动物的演代,但没有公布的魁北克省(加拿大)的数据。本研究的目的是评估魁北克省猪尸体相关节肢动物的多样性,并确定在整个分解过程中不同科/种的定殖顺序。2019年6月中旬,在trois - rivi的一小片林地中发现了三具猪尸体。在11月初(初雪)前,采用目测法,利用昆虫网和陷阱对尸体取样,研究了分解过程和节肢动物群落。在尸体上产卵的有5种蝇类(双翅目:蝇科),分别是:liliphora livida、C. vomitoria、Lucilia illustris、Phormia regina和protophoria terraenovae。前三种的卵/幼虫仅在第一周(新鲜阶段-主动腐烂开始)记录,而其他两种的卵/幼虫则在整个分解过程中记录,直到干残阶段。从第0天至第141天(试验结束)观察鞘翅目。一些科/种与分解的早期阶段有关(例如Necrophila americana, Silphidae),而其他科/种仅在干燥遗骸阶段发现(例如Carnicops pumilio, Histeridae)。
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引用次数: 8
Spitz and fisher’s medicolegal investigation of death: Guidelines for the application of pathology to crime investigation, 5th ed Spitz和fisher的死亡法医学调查:病理学在犯罪调查中的应用指南,第5版
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2020-10-01 DOI: 10.1080/10401334.2020.1838147
G. J. Davis
A confession: I wanted to like this text more than I did. I first encountered this book in its second edition while training in forensic pathology in the 80 s, and it was formative in my understand...
忏悔:我比以前更喜欢这篇文章。我第一次看到这本书是在80年代接受法医病理学培训时出版的第二版 s、 在我的理解中,它是形成性的。。。
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引用次数: 11
Effect of decontamination agents following biological contamination on fingermarks, footwear, documents and DNA 生物污染后去污剂对指纹、鞋类、文件和DNA的影响
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2020-10-01 DOI: 10.1080/00085030.2020.1834755
Della A. Wilkinson, S. Holowachuk, C. Corbett, K. Antonation, Lisa Rostek, A. Wotherspoon, K. Toole, Nathan B. Unsworth, J. Coumbaros, V. Rastogi, Brad Donais, Jackie Osmond, C. Baxter
Abstract Chemical, biological, radiological, nuclear and explosive (CBRNE) response teams are responsible for mitigating and investigating events such as the distribution of anthrax letters throughout the US Postal Service in 2001. One consideration will be the examination of contaminated physical evidence to identify those responsible. Decontamination agents are often used to inactivate or neutralize chemical and/or biological warfare agents so that once-contaminated objects can be handled safely. The objective of this project was to determine the impact of different decontamination agents on physical evidence including DNA, documents, fingermark and footwear evidence. In Phase 1, vaporous hydrogen peroxide (VHP), gamma irradiation, dry fogging, chlorine dioxide, MODEC MDF-500, Bioxy-S, ozone and formaldehyde were tested. Promising decontamination agents were selected for Phase 2 when physical evidence was exposed to Bacillus thuringiensis (Bt), an anthrax surrogate, prior to decontamination. VHP, gamma irradiation, dry fogging, formaldehyde and chlorine dioxide were tested in Phase 2. Results indicate that several forensic analyses were impacted. VHP and gamma irradiation were used across different laboratories with inconsistent results observed for some forensic protocols. Further investigation indicated that these results could be explained by variation in contamination protocols between laboratories. Les équipes d'intervention chimique, biologique, radiologique, nucléaire et explosive (CBRNE) sont chargées d'atténuer et d'enquêter sur des événements tels que la distribution de lettres d'anthrax dans l'ensemble du service postal américain en 2001. Une considération sera l'examen des preuves physiques contaminées pour identifier les responsables. Les agents de décontamination sont souvent utilisés pour inactiver ou neutraliser les agents de guerre chimiques et/ou biologiques afin que des objets autrefois contaminés puissent être manipulés en toute sécurité. L'objectif de ce projet était de déterminer l'impact de différents agents de décontamination sur les preuves physiques, y compris l'ADN, les documents, les traces de doigt et les chaussures. En phase 1, le peroxyde d'hydrogène vaporeux (VHP), l'irradiation gamma, la brumisation sèche, le dioxyde de chlore, le MODEC MDF-500, le Bioxy-S, l'ozone et le formaldéhyde ont été testés. Des agents de décontamination prometteurs ont été sélectionnés pour la phase 2 lorsque des preuves physiques ont été exposées à Bacillus thuringiensis (Bt), un substitut de l'anthrax, avant la décontamination. Le VHP, l'irradiation gamma, la brumisation sèche, le formaldéhyde et le dioxyde de chlore ont été testés à la phase 2. Les résultats indiquent que plusieurs analyses médico-légales ont été touchées. VHP et l'irradiation gamma ont été utilisée dans différents laboratoires avec des résultats incohérents observés pour certains protocoles médico-légaux. Une enquête plus approfondie a indiqué que ces résultats pour
摘要化学、生物、放射、核和爆炸物(CBRNE)响应小组负责缓解和调查2001年美国邮政局炭疽信件分发等事件。一个考虑因素是审查受污染的物理证据,以确定责任人。去污剂通常用于灭活或中和化学和/或生物战剂,以便安全处理曾经受污染的物体。该项目的目的是确定不同的去污剂对物理证据的影响,包括DNA、文件、指纹和鞋类证据。在第一阶段,测试了蒸汽过氧化氢(VHP)、γ辐射、干雾、二氧化氯、MODEC MDF-500、Bioxy-S、臭氧和甲醛。当物理证据暴露于除虫前炭疽替代物苏云金芽孢杆菌(Bt)时,为第2阶段选择了有前景的除虫剂。在第2阶段测试了VHP、γ辐射、干雾、甲醛和二氧化氯。结果表明若干法医分析受到影响。VHP和伽马辐射在不同的实验室使用,在一些法医方案中观察到不一致的结果。进一步调查表明,这些结果可以通过实验室之间污染协议的变化来解释。化学、生物、放射性、核和爆炸(CBRNE)响应小组负责减轻和调查2001年美国邮政局炭疽信件分发等事件。一个考虑因素将是审查受污染的物理证据,以确定责任人。去污剂通常用于灭活或中和化学和/或生物战剂,以便安全处理以前受污染的物体。该项目的目的是确定不同去污剂对物理证据的影响,包括DNA、文件、指纹和鞋子。在第一阶段,测试了蒸汽过氧化氢(VHP)、伽马辐射、干雾、二氧化氯、Modec MDF-500、Bioxy-S、臭氧和甲醛。当物理证据在去污前暴露于炭疽替代品苏云金芽孢杆菌(Bt)时,为第2阶段选择了有前景的去污剂。在第2阶段测试了HPV、γ辐射、干雾、甲醛和二氧化氯。结果表明,一些法医分析受到影响。VHP和伽马辐射已用于不同的实验室,在某些法医方案中观察到不一致的结果。进一步调查表明,这些结果可能是由于实验室之间污染方案的差异。
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引用次数: 4
Development of environmentally insulted fingermarks on nonporous forensically relevant substrates with conformal columnar thin films 共形柱状薄膜在无孔司法相关基底上的环境侮辱性指纹的开发
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2020-08-11 DOI: 10.1080/00085030.2020.1799658
N. Nagachar, T. Tiedge, A. Lakhtakia, M. N. McCormick, R. Roy
Abstract A systematic study was undertaken on the influence of environmental insult on the efficacy of developing a latent fingermark on a nonporous substrate by the deposition of a conformal nanoscale columnar thin film (CTF) on it. Four warm-weather and one cold-weather environmental conditions were chosen as representative for Pennsylvania. Three durations of environmental insult were selected: a day, seven days, and 30 days. Fingermarks were collected from one male donor and one female donor on five different types of substrates. The evaporant material for the deposition of a CTF on a specific fingermark sample was specifically chosen based on a prior study. Photographs of every fingermark were graded for quality after collection, after enduring the chosen environmental insult for a particular duration, and after CTF development. The results of the study on 750 fingermark samples indicate that environmental insult does not always degrade and can even improve the quality of fingermarks, and CTF deposition thereafter may considerably enhance the insulted fingermarks for visualization.
摘要系统地研究了环境损伤对在无孔基底上沉积共形纳米柱状薄膜(CTF)形成潜在指纹的效果的影响。选择宾夕法尼亚州的四个温暖天气和一个寒冷天气的环境条件作为代表。选择了三种环境损伤持续时间:一天、七天和30天 天。在五种不同类型的基质上从一名男性供体和一名女性供体身上采集指纹。用于在特定指纹样本上沉积CTF的蒸发材料是基于先前的研究专门选择的。每个指纹的照片在收集后、在特定时间内忍受选定的环境侮辱后以及CTF开发后,都会根据质量进行分级。对750个指纹样本的研究结果表明,环境损伤并不总是会退化,甚至可以提高指纹的质量,此后的CTF沉积可以显著增强受损指纹的可视化效果。
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引用次数: 6
Examining growth rates of Scopulariopsis brevicaulis on decomposing porcine remains 研究猪尸体分解后短茎细螺旋藻的生长速度
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2020-07-02 DOI: 10.1080/00085030.2020.1803030
A. Braga, W. Knaap, R. Summerbell
Abstract The purpose of this study was to examine growth rates for the filamentous fungus Scopulariopsis brevicaulis on decomposing porcine remains in relation to three controlled temperatures. This study was performed by inoculating porcine tissue samples retrieved from the same source. These samples were placed into three controlled environments to examine changes over a month. This study was intended to develop a photographic and quantifiable chronology of fungal growth that can be used as a reference by investigative personnel interested in utilizing mycological evidence in death investigations. Providing this novel information to the inadequately studied discipline of forensic mycology is intended to help establish the credibility of this field. These efforts will aid in interpreting post-mortem intervals for cadavers experiencing fungal growth in controlled indoor environments.
摘要本研究旨在研究3种控制温度下,短杆状真菌在分解猪尸体上的生长速率。本研究是通过接种同一来源的猪组织样本进行的。这些样本被放置在三个受控环境中,以检查一个月内的变化。本研究旨在开发一种真菌生长的摄影和可量化的年表,可作为对在死亡调查中利用真菌学证据感兴趣的调查人员的参考。向法医真菌学这一研究不足的学科提供这一新颖的信息,旨在帮助建立这一领域的可信度。这些努力将有助于解释在受控室内环境中经历真菌生长的尸体的死后间隔。
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引用次数: 0
First observation of burnt vertebrate carrion scavenging by black-billed magpies (Pica hudsonia (Sabine)) highlights the need to evaluate all possible scavengers at a site 首次观察到黑嘴喜鹊(Pica hudsonia (Sabine))啄食烧焦的脊椎动物腐肉,突出了评估一个地点所有可能的食腐动物的必要性
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2020-05-31 DOI: 10.1080/00085030.2020.1756117
Trevor I. Stamper, L. Pharr, G. Anderson, Carleen Gondor, Alex C Dedmon, R. Kimsey
Abstract We describe how an unusual facultative scavenger, the black-billed magpie (Pica hudsonia (Sabine)), took advantage of the compromised nature of burnt skin to scavenge porcine remains with extensive, and hitherto unreported, efficiency. Scavenging appears to be minimally reported for this species. We serendipitously made these observations while conducting a workshop on forensic entomology in Missoula, Montana, in 2015. We also illustrate how excluding larger vertebrate scavengers may reveal and highlight the taphonomic effects of smaller facultative scavengers not so excluded. Such observations could be important to forensic investigators in wildlife or homicide investigations of burnt remains.
摘要我们描述了一种不寻常的兼性清除剂,黑嘴喜鹊(Pica hudsonia(Sabine)),如何利用烧伤皮肤的受损特性,以广泛而迄今未报道的效率清除猪的遗骸。这种物种似乎很少有清除的报道。2015年,我们在蒙大拿州米苏拉举办法医昆虫学研讨会时偶然发现了这些发现。我们还说明了将较大的脊椎动物清除剂排除在外可能会揭示和强调未被排除在外的较小的兼性清除剂的taphonomic效应。这些观察结果对野生动物或焚烧遗骸凶杀案的法医调查人员来说可能很重要。
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引用次数: 1
Le transfert différentiel des fibres de coton et de polyester lors d’agressions au couteau 棉花和聚酯纤维在刀攻击时的差异转移
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2020-05-25 DOI: 10.1080/00085030.2020.1767855
Lilian Skokan, Catherine Daigle, A. Tremblay, Cyril Muehlethaler
Abstract In Canada, the knife is amongst the stabbing weapons most frequently used in the commission of violent crimes, particularly homicides. Although under-exploited, the search for fibres resulting from contact between a textile material and a receiver surface can provide relevant information on the activity at their origin. Although cotton-polyester blended textiles are among the most commonly produced worldwide, no research seems to focus on studying the proportions of fibres transferred to the knife blade during such an attack which constitutes an important lack of information for practitioners in the field. This study involved the construction of a stabbing simulation device that performed perforations under controlled parameters (e.g. force, speed and orientation of perforation) with the use of two kitchen knives with straight and serrated blades. Simulations were conducted on a set of four blended textiles to further investigate the transfer of cotton and polyester fibres and the correlation to donor textile characteristics. The transfer mainly lies on the properties of the fibres. Since cotton fibres are shorter, less resistant and less elastic than polyester fibres, they are favoured during transfer and can be found in greater proportion. Finally, the study provides some guidelines, including the addition of a scraping step, for the recovery of fibres from knife blades.
摘要在加拿大,刀是暴力犯罪,特别是凶杀案中最常用的刺伤武器之一。尽管开发不足,但对纺织材料和接收器表面之间接触产生的纤维的搜索可以提供有关其来源处活性的相关信息。尽管棉-聚酯混纺纺织品是世界上最常见的纺织品之一,但似乎没有任何研究集中在研究在这种袭击中转移到刀片上的纤维比例上,这对该领域的从业者来说是一个重要的信息缺乏。这项研究涉及一种刺入模拟装置的构建,该装置在受控参数(如穿孔的力、速度和方向)下使用两把带直刀片和锯齿刀片的菜刀进行穿孔。对一组四种混纺织物进行了模拟,以进一步研究棉花和聚酯纤维的转移以及与供体织物特性的相关性。转移主要取决于纤维的性能。由于棉纤维比聚酯纤维更短、阻力更小、弹性更小,因此在转移过程中,棉纤维更受欢迎,而且比例更大。最后,该研究为从刀片中回收纤维提供了一些指导,包括增加刮擦步骤。
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引用次数: 1
Evidential breath testing for alcohol, Parliament, the science and the courts (Part 2) 酒精呼气证据测试、议会、科学和法院(第二部分)
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2020-04-02 DOI: 10.1080/00085030.2020.1757586
G. Yost
Abstract A previous article in this journal traced the evolution of the law from the first amendment to the Criminal Code related to breath testing for alcohol in 1951 to the Supreme Court of Canada’s decision in November 2012 on the constitutionality of the 2008 Criminal Code amendments that eliminated the “two beer” defence. This article discusses the legal, scientific and parliamentary response to that decision leading up to the 2018 decision of the Supreme Court of Canada on whether maintenance records could constitute evidence that the approved instrument had malfunctioned.
摘要本杂志之前的一篇文章追溯了法律的演变,从1951年与酒精呼气测试有关的《刑法》第一修正案到2012年11月加拿大最高法院关于2008年《刑法》修正案合宪性的裁决,该修正案取消了“两杯啤酒”辩护。这篇文章讨论了法律、科学和议会对该决定的回应,该决定导致加拿大最高法院在2018年就维护记录是否可以构成批准的仪器出现故障的证据作出裁决。
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引用次数: 0
期刊
Canadian Society of Forensic Science Journal
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