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Possible dependence of sternum non-metric features on sex, age, and stature from a forensic viewpoint: a study in an Iranian population 从法医角度看胸骨非度量特征与性别、年龄和身材的可能关系:对伊朗人口的研究
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2023-12-19 DOI: 10.1080/00085030.2023.2285618
M. Ghorbanlou, Fatemeh Moradi, Hamidreza Asgari
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引用次数: 0
Background and persistence of fibers on vehicle seat belts 汽车安全带上纤维的背景和持久性
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2023-11-21 DOI: 10.1080/00085030.2023.2281006
Marie-Christine Bolduc, André Tremblay, Cyril Muehlethaler
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引用次数: 0
Intra- and inter-rater reliability of a manual codification system for footwear impressions: first lessons learned from the development of a footwear database for forensic intelligence purposes 鞋印手工编纂系统在内部和内部的可靠性:从为法医情报目的而发展鞋类数据库所吸取的初步教训
Q4 MEDICINE, LEGAL Pub Date : 2023-11-06 DOI: 10.1080/00085030.2023.2278911
Vincent Mousseau, Maralee Tapps, Romain Volery, Jean Brazeau
AbstractTo generate forensic intelligence from footwear impressions and link crime scenes, most law enforcement agencies and forensic laboratories rely on a manual codification system based on pattern recognition and classification by human analysts. However, although they are commonly used in practice, to date we still know little about the reliability of such systems. Taking advantage of the development of a footwear database for forensic intelligence purposes at the Laboratoire de sciences judiciaires et de médecine légale in Quebec (Canada), this study aims to make a preliminary assessment of the intra- and inter-rater reliability (i.e., the level of repeatability over time and the level of consensus between analysts) of the proposed codification system. To do so, three forensic intelligence analysts classified a set of 27 crime scene impressions and test impressions at two different times (two weeks apart). Percent agreement, Cohen’s Kappa, and Light’s Kappa were then calculated. Results show that two out of three analysts have reached an almost perfect level of intra-rater agreement, while the other have achieved a substantial level of intra-agreement, and that all analysts have reached a substantial level of inter-rater agreement. Findings suggest that, although a few patterns may have lower levels of agreement, overall, the developed codification system presents a satisfactory level of reliability. This preliminary study thus suggests that contrary to what advocates of fully automated systems may sometimes imply, manual codification of footwear impressions may be fairly appropriate for intelligence purposes. It calls for further evaluative research in the field.RÉSUMÉPour générer du renseignement forensique à partir des traces de chaussure et ainsi insérer la scène de crime unique dans une série criminelle, la plupart des corps policiers ont recours à un système de codification manuelle basé sur la reconnaissance et la classification de certaines formes ou motifs par des analystes formés en la matière. Bien que ces systèmes soient couramment utilisés dans la pratique quotidienne, peu d’études ont jusqu’ici tenté de cerner leur fiabilité. Profitant du développement du service de profilage de traces et d’empreintes de chaussure à des fins de renseignement criminalistique au Laboratoire de sciences judiciaires et de médecine légale au Québec (Canada), la présente étude cherche à réaliser une évaluation préliminaire de la fiabilité intra- et inter-juges (c.-à-d. le niveau de répétabilité dans le temps et le niveau de consensus entre les analystes) du système de codification manuelle élaborée. Pour ce faire, trois membres du service de renseignement criminalistique du Laboratoire ont codifié à deux reprises, à deux semaines d’intervalle, le même ensemble de 27 traces et empreintes de chaussure. Le pourcentage d’accord, le Kappa de Cohen et le Kappa de Light ont ensuite été calculés à partir des données recueillies. Les résultats révèlent que d
3 .作者承认,3名参与者的样本是一个相当小的样本,但在进行研究时,这3名分析人员是法医情报处唯一负责鞋印分析的从业人员,因为法医情报处的其他成员专门从事毒理学和毒品情报虽然他们不进行传统的法医鞋类检查和比较,但他们都接受了鞋印和轮胎胎面证据科学工作组(SWGTREAD)建议的最低限度的教育[引文40]Cohen’s Kappa和Light’s Kappa不能用恒定的模式(即所有编码条目的“0”)来计算。必须有最小的变化,以观察评级员何时识别模式,何时不识别模式,并评估评级员内部的可靠性,以最大限度地提高编纂系统的模式。这就解释了为什么为内部可靠性测试(有20个跟踪和打印)计算了比内部可靠性测试(有27个跟踪和打印)更多的度量这七张照片是根据它们在日常工作中的初始分类选出的因此,每个图案的字母数字代码(参见结果和附录)如下:括号前的字母和数字对应于图案,而括号内的字母(P, A或T)对应于观察到图案的外底部分。本研究没有从公共、商业或非营利部门的资助机构获得任何特定的资助。
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引用次数: 0
A fatal motor vehicle collision involving multiple novel psychoactive substances 一起涉及多种新型精神活性物质的致命机动车碰撞
Q4 MEDICINE, LEGAL Pub Date : 2023-11-06 DOI: 10.1080/00085030.2023.2267860
Michael Fagiola
AbstractNovel psychoactive substances comprise a number of chemically diverse substances that have emerged in the illicit market over the past decade and continue to be readily available. These compounds have been considered “legal alternatives” for typical drugs of abuse and often share similar pharmacological profiles. Presented herein is a case from the United States of a 29-year-old male with no known past medical history that was suspected of impaired driving while traveling at a high rate of speed. The vehicle stopped when the decedent lost control and collided with a guardrail. The decedent was ejected and found lying near the guardrail with extensive blunt force trauma and was pronounced deceased at the scene. Initial screening of the decedent’s cardiac blood revealed 3-methoxy-PCP, butylone, delorazepam, and N-ethylpentylone. Further testing provided by an outside reference laboratory additionally confirmed 3-fluorophenmetrazine, diclazepam, etizolam, and fluoroamphetamine. Testing of scene evidence revealed the presence of 2-fluoro-deschloroketamine, butylone, diclazepam, etizolam, and N-ethylpentylone. The cause of death was attributed to an internal hemorrhage due to a laceration of the aortic root with multiple skeletal fractures resulting from blunt force trauma. The manner of death was accidental. The compounds detected in this case may have played a significant role in facilitating this fatal crash. This report documents a unique instance of poly-NPS use, and a discussion on the analytical and interpretive considerations commonly encountered when analyzing NPS is also presented.RésuméLes nouvelles substances psychoactives comprennent un certain nombre de substances chimiquement diverses qui sont apparues sur le marché illicite au cours de la dernière décennie et qui continuent d’être facilement disponibles. Ces composés ont été considérés comme des “alternatives légales” aux drogues typiques d’abus et partagent souvent des profils pharmacologiques similaires. Nous présentons ici le cas, aux États-Unis, d’un homme de 29 ans sans antécédents médicaux connus, soupçonné de conduite sous influence alors qu’il roulait à grande vitesse. Le véhicule s’est arrêté lorsque le défunt a perdu le contrôle et est entré en collision avec une glissière de sécurité. Le défunt a été éjecté et retrouvé allongé près de la glissière de sécurité, souffrant d’un important traumatisme contondant, et son décès a été constaté sur place. L’analyse initiale du sang cardiaque du défunt a révélé la présence de 3-méthoxy-PCP, de butylone, de délorazépam et de N-éthylpentylone. D’autres tests effectués par un laboratoire externe ont confirmé la présence de 3-fluorophénmétrazine, de diclazépam, d’étizolam et de fluoroamphétamine. L’analyse des prélèvements recueillis sur scène a révélé la présence de 2-fluoro-deschlorocétamine, de butylone, de diclazépam, d’étizolam et de N-éthylpentylone. La cause du décès a été attribuée à une hémorragie interne due à une lacéra
摘要新型精神活性物质包括许多化学上不同的物质,这些物质在过去十年中出现在非法市场上,并且仍然很容易获得。这些化合物被认为是典型滥用药物的“合法替代品”,通常具有相似的药理特征。本文报告一例来自美国的29岁男性,既往病史不详,被怀疑在高速行驶时驾驶受损。当死者失去控制并撞上护栏时,车辆停了下来。死者被弹射出来,被发现躺在护栏附近,身上有大面积的钝器外伤,并在现场被宣布死亡。初步筛选死者的心脏血液发现3-甲氧基pcp,丁酮,地洛西泮和n -乙基戊酮。外部参考实验室提供的进一步检测进一步证实了3-氟苯甲曲嗪、二氮安定、依替唑仑和氟安非他明。对现场证据的检测显示存在2-氟氯氯胺酮、丁酮、二氮西泮、乙唑仑和n -乙基戊酮。死因是钝器外伤造成主动脉根部撕裂并伴有多处骨骼骨折造成的内出血。死亡方式是意外。在这起案件中检测到的化合物可能在促成这起致命事故中发挥了重要作用。本报告记录了多NPS使用的一个独特实例,并讨论了分析NPS时通常遇到的分析和解释考虑因素。在某些名称的物质混合物中,有各种各样的化学物质,例如,在某些名称的物质混合物中,有各种各样的化学物质,例如,在某些名称的物质混合物中,有各种各样的化学物质,例如,在某些名称的物质混合物中,有各种各样的化学物质。综上所述,合成的和合成的都认为,合成的和合成的都认为,合成的和合成的都认为,合成的和合成的都认为是“替代的”。常识presentons ici cas,在美国,一个人德29 ans sans祖先medicaux有名,soupconne de conduite那么只要有了格兰德维特斯roulait真空的影响。Le vachidule s 'est arrêté lorsque Le dachidule a perdu Le contrôle et est entrentre en collision avec une glisis re de sachidule 。danci.911 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.917 - anci.924。我分析了初始值du sang cardiaque du danci.achique du danci.acdique du danci.acdique和danci.acdique。D ' autres测试了有效的薪金薪金,并在实验室外部确认了3-氟型薪金薪金、2 -氟型薪金薪金、3-氟型薪金薪金和4 -氟型薪金薪金薪金的薪金薪金。分析了2-氟-去氯胺、丁酮、二氯胺、去氯胺和N-甲基戊酮的合成和合成。a cause du danci.9cha.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com.com。Le mode de dsamicous est accidental。综上所列,所有的人都有自己的职业生涯和职业生涯,但重要的人都有自己的职业生涯。该协议文件提出了多元nps的独特应用,并对多元nps在全球范围内的应用效果进行了讨论。关键词:新型精神活性物质;损害驾驶;法医毒理学;死后多物质使用;mots类;新物质;精神活性;其他信息资金作者报告没有与本文所述工作相关的资金。
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引用次数: 0
Individual age estimation using pulp-to-tooth area ratio in single-rooted teeth 单根牙牙髓牙面积比估算个体年龄
Q4 MEDICINE, LEGAL Pub Date : 2023-10-30 DOI: 10.1080/00085030.2023.2276562
Matthieu Trottier, Scott Keenan, Scott I. Fairgrieve
AbstractDental age estimation can play a crucial role in forensic investigations as it can assist authorities in the identification of living and deceased individuals. Of the various age estimation methods based on odontology, pulp-to-tooth ratios measured through radiography have been a popular choice due to their less invasive nature. Pulp-to-tooth area ratios were assessed in 12 permanent single rooted teeth (maxillary and mandibular, left and right central incisors, lateral incisors, and canines) using clinical cone beam computed tomographs. A total of 227 teeth were analyzed from 66 subjects, 33 males and 33 females. Pulp-to-tooth area ratios were measured in the coronal, sagittal and axial perspectives. Additionally, the difference in pulp-to-tooth area ratio between the entire pulp cavity area of the tooth, and the pulp cavity area exclusively above the cementoenamel junction were compared. Bilateral symmetry was confirmed between the teeth from the right and left side (p = 0.9405). All correlations exceeded 0.6, with the highest correlations observed in maxillary teeth (R=-0.836), the teeth from females (R=-0.830), and central and lateral incisors exclusively (R=-0.802). The standard error of estimates from the linear regression models varied between ±10.11 and 14.98 years. This study confirmed that pulp-to-tooth ratios are a sound technique to estimate age.RÉSUMÉL’estimation d’âge dentaire peut jouer un rôle important dans les enquêtes médicolégales, car elle peut aider les autorités à identifier des individus vivants et décédés. Parmi les maintes méthodes d’estimation d’âge pour la dentition, le ratio de pulpe-à-dent mesuré à partir d’interprétations radiographiques demeure favorable à cause de la technique moins invasive. Le ratio de pulpe-à-dent fut analysé dans 12 dents permanentes à racine unique (incisive centrale et latérale, et la canine, de la maxillaire et mandibulaire et de la droite et gauche). Un total de 227 dents fut analysé parmi 66 sujets, dont 33 hommes et 33 femmes. Le ratio de pulpe-à-dent fut mesuré dans le plan coronal, sagittal et axial. De plus, la différence en ratio de pulpe-à-dent fut comparée entre la pulpe complète de la dent et la pulpe supérieure à la jonction amélo-cémentaire. La symétrie bilatérale fut confirmée entre les dents de la droite et de la gauche (p = 0,9405). Toutes les corrélations ont surpassé 0,6 avec la plus haute corrélation notée dans les dents maxillaires (R=-0,836), les dents des femmes (R=-0,830), et les incisives centrales et latérales uniquement (R=-0,802). L'erreur type d’estimation des modèles de régression linéaire a varié entre ±10,11 et 14,98 années. Cette étude confirme que les ratios de pulpe-à-dent présentent une technique propice à estimer l’âge.Keywords: Forensic scienceForensic odontologyAge estimationPulp-to-tooth ratioSecondary dentinCBCTMOTS CLÉS: Sciences medicolégalesodontologie medicolégalesestimation d’âgeratio de pulpe-à-dentdentine secondairetomographie volumique
摘要牙齿年龄估计在法医调查中起着至关重要的作用,因为它可以帮助当局识别活着和死去的人。在基于牙科学的各种年龄估计方法中,通过x线摄影测量牙髓与牙齿的比例由于其侵入性较小而成为一种流行的选择。采用临床锥束计算机断层扫描技术评估12颗永久单根牙(上颌和下颌、左右中门牙、侧门牙和犬齿)的牙髓-牙面积比。共分析了66名受试者(33名男性和33名女性)的227颗牙齿。在冠状面、矢状面和轴向面测量牙髓与牙齿的面积比。此外,我们还比较了牙齿的整个牙髓腔面积和牙髓-牙釉质交界处以上的牙髓腔面积的牙髓-牙髓面积比的差异。左右侧牙对称(p = 0.9405)。相关性均大于0.6,其中上颌牙相关性最高(R=-0.836),女性牙相关性最高(R=-0.830),中门牙和侧门牙相关性最高(R=-0.802)。线性回归模型估计的标准误差在±10.11 ~ 14.98年之间。这项研究证实,牙髓与牙齿的比例是一个可靠的技术来估计年龄。RÉSUMÉL ' estimationd ' ge dentaire peut jouer un rôle重要的dans les enquêtes msamdicolsamgales, car elle peut aider les autoritsamuys, identifier des individual viants et dsamued.com。Parmi les maintes msamthodes d ' estimate d ' ge pour la dentition, le ratio de pulpe-à-dent mesur partir d ' interpraciments radigrapques demeure有利,因为de la technology运动具有侵入性。Le ratio de pulpe-à-dent fut analysisdans 12 dent permanentes racine unique(锐利的centrale et lat薪金,et la canine, de la maxillaire et mandibulaire and de la droite et gauche)。共有227名学生参与了66项研究,其中33名是男性,33名是女性。Le ratio de pulpe-à-dent fut measurements . dans Le plan冠状、矢状及轴状。另外,la difference en ratio De pulmente -à-dent fut compaceme entre la pulpe complante De la dent et la pulpe supsamriue eure la jontion amsamlo - csammentaire。对称的双侧的双侧的横向的和横向的(p = 0,9405)。其中,较低的薪金薪金超过了平均薪金的0.6倍,加上较高的薪金薪金,如薪金薪金、薪金薪金、薪金薪金(R=- 0.836)、薪金薪金、薪金薪金(R=- 0.830)、薪金薪金和薪金薪金(R=- 0.0802)。L’erreur d’型估计:对 (10、11)和(14、98)个 (14、98)个。Cette 技术估算器(estimer l ' ge)。关键词:法医scienceForensic odontologyAge estimationPulp-to-tooth ratioSecondary dentinCBCTMOTS cl:科学medicolegalesodontologie medicolegalesestimation d 'ageratio de pulpe-a-dentdentine secondairetomographie volumique一faisceau conique statementNo披露潜在的利益冲突是报告的作者。其他信息资金作者报告没有与本文所述工作相关的资金。
{"title":"Individual age estimation using pulp-to-tooth area ratio in single-rooted teeth","authors":"Matthieu Trottier, Scott Keenan, Scott I. Fairgrieve","doi":"10.1080/00085030.2023.2276562","DOIUrl":"https://doi.org/10.1080/00085030.2023.2276562","url":null,"abstract":"AbstractDental age estimation can play a crucial role in forensic investigations as it can assist authorities in the identification of living and deceased individuals. Of the various age estimation methods based on odontology, pulp-to-tooth ratios measured through radiography have been a popular choice due to their less invasive nature. Pulp-to-tooth area ratios were assessed in 12 permanent single rooted teeth (maxillary and mandibular, left and right central incisors, lateral incisors, and canines) using clinical cone beam computed tomographs. A total of 227 teeth were analyzed from 66 subjects, 33 males and 33 females. Pulp-to-tooth area ratios were measured in the coronal, sagittal and axial perspectives. Additionally, the difference in pulp-to-tooth area ratio between the entire pulp cavity area of the tooth, and the pulp cavity area exclusively above the cementoenamel junction were compared. Bilateral symmetry was confirmed between the teeth from the right and left side (p = 0.9405). All correlations exceeded 0.6, with the highest correlations observed in maxillary teeth (R=-0.836), the teeth from females (R=-0.830), and central and lateral incisors exclusively (R=-0.802). The standard error of estimates from the linear regression models varied between ±10.11 and 14.98 years. This study confirmed that pulp-to-tooth ratios are a sound technique to estimate age.RÉSUMÉL’estimation d’âge dentaire peut jouer un rôle important dans les enquêtes médicolégales, car elle peut aider les autorités à identifier des individus vivants et décédés. Parmi les maintes méthodes d’estimation d’âge pour la dentition, le ratio de pulpe-à-dent mesuré à partir d’interprétations radiographiques demeure favorable à cause de la technique moins invasive. Le ratio de pulpe-à-dent fut analysé dans 12 dents permanentes à racine unique (incisive centrale et latérale, et la canine, de la maxillaire et mandibulaire et de la droite et gauche). Un total de 227 dents fut analysé parmi 66 sujets, dont 33 hommes et 33 femmes. Le ratio de pulpe-à-dent fut mesuré dans le plan coronal, sagittal et axial. De plus, la différence en ratio de pulpe-à-dent fut comparée entre la pulpe complète de la dent et la pulpe supérieure à la jonction amélo-cémentaire. La symétrie bilatérale fut confirmée entre les dents de la droite et de la gauche (p = 0,9405). Toutes les corrélations ont surpassé 0,6 avec la plus haute corrélation notée dans les dents maxillaires (R=-0,836), les dents des femmes (R=-0,830), et les incisives centrales et latérales uniquement (R=-0,802). L'erreur type d’estimation des modèles de régression linéaire a varié entre ±10,11 et 14,98 années. Cette étude confirme que les ratios de pulpe-à-dent présentent une technique propice à estimer l’âge.Keywords: Forensic scienceForensic odontologyAge estimationPulp-to-tooth ratioSecondary dentinCBCTMOTS CLÉS: Sciences medicolégalesodontologie medicolégalesestimation d’âgeratio de pulpe-à-dentdentine secondairetomographie volumique ","PeriodicalId":44383,"journal":{"name":"Canadian Society of Forensic Science Journal","volume":"175 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136067898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A preliminary study evaluating the relationship between force and incised trauma on pig rib bones 力与猪肋骨切口创伤关系的初步研究
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2023-09-08 DOI: 10.1080/00085030.2023.2255410
Cathy Ngọc Hân Tran, Eugene Liscio
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引用次数: 0
A fluorescent enhancement method for footwear impressions in ice melt products 一种用于冰融化产品鞋类压痕的荧光增强方法
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2023-08-31 DOI: 10.1080/00085030.2023.2240667
A. Thompson, Zahra M. Linsky, Lindsey A. Welch
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引用次数: 0
Cadaver transport in large river systems: winter case study in the South Saskatchewan River 大型河流系统中的尸体运输:南萨斯喀彻温河冬季案例研究
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2023-08-01 DOI: 10.1080/00085030.2023.2243015
Iain D. Phillips, Ernie Walker
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引用次数: 0
Evaluation of the Intoxilyzer® 9000 evidential breath alcohol testing instrument Intoxilyzer®9000呼气酒精测试仪的评估
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2023-07-26 DOI: 10.1080/00085030.2023.2230012
I. Bugyra, B. Cahill, T. L. Martin
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引用次数: 0
Simultaneous screening and quantitation of stimulant drugs in dried blood spot (DBS) samples using ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) 超高效液相色谱四极杆飞行时间质谱(UPLC-QTOF-MS)同时筛选和定量干血斑(DBS)样品中的兴奋剂
IF 0.4 Q4 MEDICINE, LEGAL Pub Date : 2023-06-12 DOI: 10.1080/00085030.2023.2171022
K. Unger, J. Watterson
Abstract Dried blood spots (DBS) have seen recent development as an alternative to whole blood samples for drug testing. The ease of use of DBS makes the technique suitable and attractive for drug testing applications. Quantitation of drug concentrations in the ng/mL range can be achieved from DBS samples using 10 to 20 µL of blood. The establishment of drug per se limits for drivers requires timely methods of sampling blood for accurate drug concentration measurements. Method validation for the analysis of forensically relevant stimulant drugs and their metabolites in DBS samples by ultra-performance liquid chromatography-quadrupole time-of-flight high-resolution mass spectrometry (UPLC-QTOF-MS) is presented. DBS blood samples were prepared by spiking 20 µL of blood-containing analytes ranging from 10 to 1000 ng/mL onto Whatman® 903 Protein Saver cards. Analytes were extracted from whole DBS samples punched from protein saver cards by sonication in water followed by PRiME® MCX μElution solid phase extraction. An LOQ of 10 ng/mL was achieved for all analytes. Precision (0.09% to 14.67%), bias (–17.15% to 13.91%) and matrix effects (–18.7% to 23.4%) were suitable for validation in 10/14 analytes. DBS drug stability varied by analyte. DBS sampling may assist in overcoming forensic challenges associated with blood sampling, and accurate drug quantitation even at low concentrations is possible. The results of this work suggest that DBS microsampling is suitable for drug-impaired driving cases in jurisdictions where per se limits for drugs exist, particularly for zero-tolerance drug limits. Abstrait Les gouttes de sang séché (DBS) ont vu le développement récent comme une alternative aux échantillons de sang total pour le dépistage des drogues. La facilité d’utilisation de DBS rend la technique appropriée et attrayante pour les applications de dépistage de drogues. La quantification des concentrations de médicament dans la gamme ng/mL peut être obtenue à partir d’échantillons DBS en utilisant 10 à 20 μL de sang. L’établissement de limites de drogue en soi pour les conducteurs nécessite des méthodes opportunes d’échantillonnage du sang pour des mesures précises de la concentration de drogue. La validation de la méthode pour l’analyse de médicaments stimulants pertinents sur le plan médico-légal et de leurs métabolites dans des échantillons de DBS par chromatographie liquide ultra-performante-spectrométrie de masse à temps de vol quadripolaire à haute résolution (UPLC-QTOF-MS) est présentée. Des échantillons de sang DBS ont été préparés en dopant 20 μL d’analytes contenant du sang allant de 10 à 1000 ng/mL sur des cartes Whatman® 903 Protein Saver. Les analytes ont été extraits d’échantillons entiers de DBS perforés à partir de cartes économes en protéines par sonication dans l’eau suivie d’une extraction en phase solide PRiME® MCX μElution. Une LOQ de 10 ng/mL a été atteinte pour tous les analytes. La précision (0,09 % à 14,67 %), le biais (–17,15 % à 1
摘要干血点(DBS)最近被视为药物检测全血样本的替代品。DBS的易用性使该技术适用于药物测试应用并具有吸引力。使用10至20µL血液的DBS样品可实现ng/mL范围内药物浓度的定量。建立驾驶员的药物本身限制需要及时取样血液以进行准确的药物浓度测量。介绍了通过超高效液相色谱-四极飞行时间高分辨率质谱(UPLC-QTOF-MS)分析DBS样品中法医相关兴奋剂及其代谢物的方法验证。DBS血液样本是通过在Whatman上喷洒20µl含血分析物制备的,范围为10至1000 ng/ml® 903张蛋白质保护卡。分析物是从整个DBS样品中提取的,通过水超声从蛋白质保存卡中冲出,随后是PRIME® MCXμ洗脱固相萃取。所有分析物均达到10 ng/ml的LOQ。精度(0.09%至14.67%)、偏差(17.15%至13.91%)和矩阵效应(18.7%至23.4%)适用于10/14分析物的验证。DBS药物稳定性因分析物而异。DBS采样有助于克服与血液采样相关的法医挑战,即使在低浓度下也可能进行精确的药物定量。这项工作的结果表明,DBS微安培适用于存在药物限制的司法管辖区的药物损害驾驶案例,特别是零耐受药物限制。摘要:干血滴(DBS)已被视为药物检测全血样本的替代品。DBS的易用性使该技术适用于药物检测应用并具有吸引力。可使用10至20μl血液从DBS样品中获得ng/ml范围内药物浓度的定量。为驾驶员设定药物限制本身需要及时的血液取样方法,以准确测量药物浓度。介绍了通过高效液相色谱-高分辨率四极飞行时间质谱(UPLC-QTOF-MS)分析DBS样品中法医相关刺激药物及其代谢物的方法验证。通过在Whatman卡上掺杂20μl含有10至1000 ng/ml血液的分析物来制备DBS血液样本。® 903蛋白质保护剂。分析物通过在水中超声处理,然后进行高级固相提取,从蛋白质经济卡中的穿孔DBS的整个样品中提取。® MCXμ洗脱。所有分析物的LOQ均达到10ng/ml。准确度(0.09%至14.67%)、偏差(-17.15%至13.91%)和矩阵效应(-18.7%至23.4%)适用于10/14分析物的验证。药物DBS的稳定性因分析物而异。DBS取样有助于克服与血液取样相关的法医挑战,即使在低浓度下也可以精确定量药物。这项工作的结果表明,DBS微观抽样适用于药物本身存在限制的司法管辖区的药物损害驾驶案例,特别是零容忍度限制。
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引用次数: 0
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Canadian Society of Forensic Science Journal
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